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Antibodies provide a sensitive indicator of proteins displayed by bacteria during sepsis. Because signals produced by infection are naturally amplified during the antibody response, host immunity can be used to identify biomarkers for proteins that are present at levels currently below detectable limits. We developed a microarray comprising approximately 70% of the 4066 proteins contained within the Yersinia pestis proteome to identify antibody biomarkers distinguishing plague from infections caused by other bacterial pathogens that may initially present similar clinical symptoms. We first examined rabbit antibodies produced against proteomes extracted from Y. pestis, Burkholderia mallei, Burkholderia cepecia, Burkholderia pseudomallei, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella flexneri, and Escherichia coli, all pathogenic Gram-negative bacteria. These antibodies enabled detection of shared cross-reactive proteins, fingerprint proteins common for two or more bacteria, and signature proteins specific to each pathogen. Recognition by rabbit and non-human primate antibodies involved less than 100 of the thousands of proteins present within the Y. pestis proteome. Further antigen binding patterns were revealed that could distinguish plague from anthrax, caused by the Gram-positive bacterium Bacillus anthracis, using sera from acutely infected or convalescent primates. Thus, our results demonstrate potential biomarkers that are either specific to one strain or common to several species of pathogenic bacteria.

In this study an industrial Saccharomyces cerevisiae yeast strain capable of fermenting ethanol from pretreated lignocellulosic material was engineered. Genes encoding cellulases (endoglucanase, exoglucanase and β-glucosidase) were integrated into the chromosomal ribosomal DNA and delta regions of a derivative of the K1-V1116 wine yeast strain. The engineered cellulolytic yeast produces ethanol in one step through simultaneous saccharification and fermentation of pretreated biomass without the addition of exogenously produced enzymes. When ethanol fermentation was performed with 10% dry weight of pretreated corn stover, the recombinant strain fermented 63% of the cellulose in 96 h and the ethanol titer reached 2.6% v/v. These results demonstrate that cellulolytic S. cerevisiae strains can be used as a platform for developing an economical advanced biofuel process.

Yogurt starter and probiotic bacteria have been reported to confer health benefits to the consumer; however, to confer these health benefits yogurt and probiotic bacteria should be live and present at the recommended concentration of 6 to 8 log cfu g-1. Cegemett® Fresh (Cognis Nutrition & Health, Monheim, Germany) is a plant extract that possesses antioxidant properties. This research was divided into two experiments. The objective of experiment-I was to investigate the effect of plant extract supplementation on the redox potential (Eh) and the viability of starter cultures (Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus) in nonfat yogurt. Five yogurt samples [non-supplemented, supplemented with 0.5 or 1.0% (w/v) plant extract, or supplemented with 0.014 or 0.028% (w/w) L-cysteine. HCl] were prepared, stored at 5°C for 50 days and analyzed weekly. L. bulgaricus counts in supplemented yogurts were> 6 log cfu mL-1 for additional 7 to 21 days compared with non-supplemented yogurt; however, S. thermophilus counts in all yogurts were> 6 log cfu mL-1 throughout the storage. Overall, Eh of plant extract supplemented yogurts were similar to non-supplemented yogurt during storage; therefore the improvement in L. bulgaricus viability cannot be attributed to the Eh alone. The objective of experiment-II was to investigate the effect of plant extract supplementation on the buffering ability of the yogurt mix, and on the viability of starter and probiotic (Bifidobacterium animalis ssp. animalis and Lactobacillus acidophilus) cultures in nonfat yogurt stored at 5°C for 50 days. Nine yogurt samples were prepared with 0.5% (w/v) plant extract, 0.25% (w/v) sodium acetate or no supplement, fermented with starter cultures and B. animalis, L. acidophilus or both probiotics, and analyzed weekly. The plant extract and sodium acetate supplemented yogurt mixes had greater buffering capacities compared with non-supplemented yogurt mix. L. bulgaricus and L. acidophilus counts in supplemented yogurts were> 6 log cfu mL-1 for additional 7 to 35 days compared with non-supplemented yogurts. S. thermophilus and B. animalis counts were not affected by supplementation. These results suggested that greater buffering capacity could improve the viability of L. bulgaricus and L. acidophilus in yogurt during storage.

More and more pharma and biotech companies are realising the value of taking on experienced personnel on a short-term basis, to do project work, to fill in while a permanent recruitment is made and to add value to an existing position. It is also becoming more common these days that younger executives are seeking out interim positions as a career, since they enjoy the new challenges and flexibility of the appointments. The experience that an interim manager can bring into a company, on a short-term assignment, cannot be over-estimated and more and more bio-pharma organisations are realising this.