404 results on '"Mice as laboratory animals -- Research"'
Search Results
2. Caloric restriction decreases survival of aged mice in response to primary influenza infection
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Gardner, Elizabeth M.
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Mice as laboratory animals -- Research ,Influenza -- Research ,Health ,Seniors - Abstract
Caloric restriction (CR) extends life span of healthy rodents compared to those fed ad libitum. Previous studies have shown positive effects of CR on the immune response of aged mice after influenza immunization. To extend these studies, a mouse model of CR was used to determine if CR could modulate primary responses of aged mice to influenza. Although CR delayed the age-related decrease in mitogen-induced lymphoproliferation of aged mice, in stark contrast, CR decreased survival, increased virus titers, and reduced natural killer cell activity in lungs of aged mice after primary influenza infection. Thus, CR has differential effects on immunity of aged mice, as general indices of immune response are maintained, but primary responses to influenza infection are impaired. This suggests that, although CR may positively affect many long-term parameters of aging, increased susceptibility after primary exposure of aged mice to virus, such as influenza, may not be correctable by CR.
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- 2005
3. Caveolin-3 knockout mice show increased adiposity and whole body insulin resistance, with ligand-induced insulin receptor instability in skeletal muscle
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Capozza, Franco, Combs, Terry P., Cohen, Alex W., Cho, You-Ree, Park, So-Young, Schubert, William, Williams, Terence M., Brasaemle, Dawn L., Jelicks, Linda A., Scherer, Philipp E., Kim, Jason K., and Lisanti, Michael P.
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Mice as laboratory animals -- Research ,Insulin resistance -- Research ,Insulin resistance -- Physiological aspects ,Caveolins -- Research ,Caveolins -- Physiological aspects ,Muscles -- Research ,Muscles -- Physiological aspects ,Biological sciences - Abstract
Caveolin-3 (Cav-3) is expressed predominantly in skeletal muscle fibers, where it drives caveolae formation at the muscle cell's plasma membrane. In vitro studies have suggested that Cav-3 may play a positive role in insulin signaling and energy metabolism. We directly address the in vivo metabolic consequences of genetic ablation of Cav-3 in mice as it relates to insulin action, glucose metabolism, and lipid homeostasis. At age 2 mo, Cav-3 null mice are significantly larger than wild-type mice, and display significant postprandial hyperinsulinemia, whole body insulin resistance, and whole body glucose intolerance. Studies using hyperinsulinemic-euglycemic clamps revealed that Cav-3 null mice exhibited 20% and 40% decreases in insulin-stimulated whole body glucose uptake and whole body glycogen synthesis, respectively. Whole body insulin resistance was mostly attributed to 20% and 40% decreases in insulin-stimulated glucose uptake and glucose metabolic flux in the skeletal muscle of Cav-3 null mice. In addition, insulin-mediated suppression of hepatic glucose production was significantly reduced in Cav-3 null mice, indicating hepatic insulin resistance. Insulin-stimulated glucose uptake in white adipose tissue, which does not express Cav-3, was decreased by ~70% in Cav-3 null mice, suggestive of an insulin-resistant state for this tissue. During fasting, Cav-3 null mice possess normal insulin receptor protein levels in their skeletal muscle. However, after 15 min of acute insulin stimulation, Cav-3 null mice show dramatically reduced levels of the insulin receptor protein, compared with wild-type mice treated identically. These results suggest that Cav-3 normally functions to increase the stability of the insulin receptor at the plasma membrane, preventing its rapid degradation, i.e., by blocking or slowing ligand-induced receptor downregulation. Thus our results demonstrate the importance of Cav-3 in regulating whole body glucose homeostasis in vivo and its possible role in the development of insulin resistance. These findings may have clinical implications for the early diagnosis and treatment of caveolinopathies. limb girdle muscular dystrophy; glucose intolerance; hyper-insulinemia; insulin receptor degradation
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- 2005
4. Dietary (n-3) polyunsaturated fatty acids do not affect the in vivo development and function of Listeria-specific [CD4.sup.+] and [CD8.sup.+] effector and memory/effector T cells in mice
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Irons, Robert, Pinge-Filho, Phileno, and Fritsche, Kevin L.
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T cells -- Research ,Mice as laboratory animals -- Research ,Fatty acids -- Research ,Food/cooking/nutrition - Abstract
We previously reported that in a mouse model, a diet high in (n-3) PUFA diminishes host survival following an infection from Listeria monocytogenes, a gram-positive bacterial pathogen. In this study we investigated the impact of (n-3) PUFA on the adaptive immune response to L. monocytogenes. BALB/c mice were fed experimental diets either devoid of or rich in (n-3) PUFA from fish oil for 4 wk and then infected with [10.sup.6] actA-deficient L. monocytogenes. At 7 and 35 d postchallenge, effector and memory/effector T cells in the spleen were enumerated by flow cytometry. Surprisingly, the number of Listeria-specific [CD4.sup.+] and [CD8.sup.+] effector and memory/effector T cells in the spleen was not affected by (n-3) PUFA. Also, the effector cells derived from mice fed either diet were equally capable of conferring protective immunity upon adoptive transfer to naive recipients. Despite our previous data, which demonstrated that (n-3) PUFA profoundly impaired host resistance to L. monocytogenes, pathogen-specific T cell responses were not substantially affected by dietary (n-3) PUFA. KEY WORDS: * (-3) PUFA * fatty acid * infection * Listeria * T lymphocytes * mice
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- 2005
5. Dietary genistein improves survival and reduces expression of osteopontin in the prostate of transgenic mice with prostatic adenocarcinoma (TRAMP)
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Mentor-Marcel, Roycelynn, Lamartiniere, Coral A., Eltoum, Isam A., Greenberg, Norman M., and Elgavish, Ada
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Prostate cancer -- Research ,Mice as laboratory animals -- Research ,Isoflavones -- Research ,Food/cooking/nutrition - Abstract
Studies in vitro suggest that osteopontin (OPN), an extracellular matrix protein secreted by macrophages infiltrating prostate tumors, and by tumor cells, may have a role in the transition from clinically insignificant tumors to metastatic prostate cancer (PC). Latent PC occurs at equal rates in Western and Asian men, but the incidence of advanced PC is many-fold higher in Western men. Our earlier studies in TRAnsgenic Mouse Prostate adenocarcinoma (TRAMP) mice showed that genistein, an isoflavone found in soybeans, lowered the incidence of advanced PC. This suggested that lower intake of dietary soy may be one possible cause for higher incidence of advanced PC in Western men. The objective of the present study was to test the hypothesis that genistein may exert its preventive effect by inhibiting OPN expression. From 5 to 28 wk of age, 80, 68, and 30 TRAMP mice were fed AIN-76A diet containing 0, 250, or 500 mg genistein/kg body weight, respectively. Organ weights were measured. The steady-state level of OPN mRNA was evaluated by RT-PCR in a longitudinal study in 74 TRAMP and 32 nontransgenic litter mates (NTM). Administration of 250 and 500 mg genistein/kg AIN-76A improved survival (P = 0.008 and P = 0.005, respectively) and reduced mean weight of prostates with poorly differentiated cancer (PD) (P < 0.001), as well as the mean weight of periaortic lymph nodes (LN), although the latter was not significant. OPN was upregulated 10-fold in PD compared with prostates with a lower pathological score from TRAMP or NTM of any age (P = 0.003). OPN mRNA levels in the dorsolateral prostate and metastasis to LN were significantly correlated (r = 0.643; P = 0.00006). Genistein had a dose-dependent, significant inhibitory effect on OPN transcript levels in prostates displaying advanced prostate cancer (PD; score 6; P = 0.05). Studies are consistent with the possibility that dietary genistein may delay the progression from benign to malignant tumors by inhibiting OPN expression. KEY WORDS: * genistein * prostate * osteopontin * TRAMP * cancer progression
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- 2005
6. Marginal maternal biotin deficiency in CD-1 mice reduces fetal mass of biotin-dependent carboxylases
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Sealey, Wendy M., Stratton, Shawna L., Mock, Donald M., and Hansen, Deborah K.
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Mice as laboratory animals -- Research ,Birth defects -- Research ,Biotin -- Research ,Vitamin deficiency -- Research ,Vitamin deficiency -- Risk factors ,Food/cooking/nutrition - Abstract
Marginal maternal biotin deficiency reduces hepatic activity of biotin-dependent carboxylases and causes high rates of fetal birth defects in mice. We tested the hypothesis that the decreased carboxylase activity observed in deficient dams and their offspring is mediated by decreased abundance of biotinylated carboxylases, decreased expression of their mRNAs, or both. During gestation, CD-1 mice were fed a diet that induced biotin deficiency or a biotin-sufficient diet. On gestational d 17, gravid uteri were removed, and each live fetus was examined grossly for defects. The expected high incidence of cleft palate (83%) in offspring was observed. In maternal and fetal liver, acetyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and [beta]-methylcrotonyl-CoA carboxylase abundances were determined by Western blotting; the content of mRNAs for most of these enzymes and holocarboxylase synthetase was determined by real-time RT-PCR. Biotin deficiency significantly reduced the abundance of the carboxylases in maternal and fetal liver; neither the content of mRNAs for the carboxylases nor holocarboxylase synthetase changed. This study provides evidence that the decrease in carboxylase activities is attributable to a decrease in the abundance of biotinylated carboxylases; further, this effect is more severe in fetuses than dams. KEY WORDS: * biotin * biotin-dependent carboxylases * CD-1 mice * mRNA
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- 2005
7. Neuronal migration in the murine rostral migratory stream requires serum response factor
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Alberti, Siegfried, Krause, Sven M., Kretz, Oliver, Philippar, Ulrike, Lemberger, Thomas, Casanova, Emilio, Wiebel, Franziska F., Schwarz, Heinz, Frotscher, Michael, Schutz, Gunther, and Nordheim, Alfred
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Central nervous system -- Research ,Brain -- Research ,Cytoskeleton -- Research ,Mice as laboratory animals -- Research ,Science and technology - Abstract
The central nervous system is fundamentally dependent on guided cell migration, both during development and in adulthood. We report an absolute requirement of the transcription factor serum response factor (SRF) for neuronal migration in the mouse forebrain. Conditional, late-prenatal deletion of Srf causes neurons to accumulate ectopically at the subventricular zone (SVZ), a prime neurogenic region in the brain. SRF-deficient ceils of the SVZ exhibit impaired tangential chain migration along the rostral migratory stream into the olfactory bulb. SVZ explants display retarded chain migration in vitro. Regarding target genes, SRF deficiency impairs expression of the [beta]-actin and gelsolin genes, accompanied by reduced cytoskeletal actin fiber density. At the posttranslational level, cofilin, a key regulator of actin dynamics, displays dramatically elevated inhibitory phosphorylation at Ser-3. Our studies indicate that SRF-controlled gene expression directs both the structure and dynamics of the actin microfilament, thereby determining cell-autonomous neuronal migration. actin cytoskeleton | cofilin | transcription
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- 2005
8. Strain-dependent differences in responses to exercise training in inbred and hybrid mice
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Massett, Michael P. and Berk, Bradford C.
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Mice as laboratory animals -- Research ,Mice as laboratory animals -- Physiological aspects ,Exercise -- Research ,Exercise -- Physiological aspects ,Biological sciences - Abstract
The aim of this study was to characterize the response to exercise training in several mouse strains and estimate the genetic contribution to phenotypic variation in the responses to exercise training. Male mice from three inbred strains [C57B1/6J (BL6), FVB/NJ (FVB), and Balb/cJ (Balb/c)] and three hybrid [F.sub.1] strains [CB6F1/J (CB6 = female Balb/c x male BL6), B6F [F.sub.1] (female BL6 x male FVB), and FB6 [F.sub.1] (female FVB x male BL6)] completed an exercise performance test before and after a 4-wk treadmill running program. Distance was used as the primary estimate of endurance exercise performance. FVB mice showed the greatest response to training, with five- to sevenfold greater increases in distance run compared with BL6 and Balb/c strains. Specifically, BL6, FVB, and Balb/c strains increased distance by 33, 172, and 23%, respectively. A similar pattern of changes across strains was observed for run time (17, 87, and 11%) and work (99, 287, and 57%). As a group, [F.sub.1] hybrid mice derived from BL6 and FVB strains showed an intermediate response to training (61%). However, further analysis indicated that training responses in FB6 [F.sub.1] mice (80%) were ~2.5-fold greater than responses in B6F [F.sub.1] mice (33%, P = 0.08). A similar pattern of changes between FB6 and B6F [F.sub.1] mice was observed for run time (44.5 and 17%) and work (141 and 59%). These data demonstrate that there are large strain-dependent differences in training responses among inbred mouse strains, suggesting that genetic background contributes significantly to adaptation to exercise. Furthermore, the contrasting responses in B6F and FB6 [F.sub.1] strains show that a maternal component strongly influences strain-dependent differences in training responses. treadmill running; hybrid mouse strains; broad-sense heritability
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- 2005
9. Protective effect of hawthorn fruit on murine experimental colitis
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Fujisawa, Min, Oguchi, Katsuji, and Yamaura, Taku
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Mice as laboratory animals -- Research ,Hawthorns -- Research ,Colitis -- Care and treatment ,Colitis -- Research ,Health - Published
- 2005
10. Genotype-environment interactions in mouse behavior: a way out of the problem
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Kafkafi, Neri, Benjamini, Yoav, Sakov, Anat, Elmer, Greg I., and Golani, Ilan
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Mice as laboratory animals -- Research ,Mice as laboratory animals -- Genetic aspects ,Mice as laboratory animals -- Behavior ,Behavior genetics -- Research ,Behavior genetics -- Practice ,Science and technology - Abstract
In behavior genetics, behavioral patterns of mouse genotypes, such as inbred strains, crosses, and knockouts, are characterized and compared to associate them with particular gene loci. Such genotype differences, however, are usually established in single-laboratory experiments, and questions have been raised regarding the replicability of the results in other laboratories. A recent multilaboratory experiment found significant laboratory effects and genotype x laboratory interactions even after rigorous standardization, raising the concern that results are idiosyncratic to a particular laboratory. This finding may be regarded by some critics as a serious shortcoming in behavior genetics. A different strategy is offered here: (i) recognize that even after investing much effort in identifying and eliminating causes for laboratory differences, genotype x laboratory interaction is an unavoidable fact of life. (ii) Incorporate this understanding into the statistical analysis of multilaboratory experiments using the mixed model. Such a statistical approach sets a higher benchmark for finding significant genotype differences. (iii) Develop behavioral assays and endpoints that are able to discriminate genetic differences even over the background of the interaction. (iv) Use the publicly available multilaboratory results in single-laboratory experiments. We use software-based strategy for exploring exploration (SEE) to analyze the open-field behavior in eight genotypes across three laboratories. Our results demonstrate that replicable behavioral measures can be practically established. Even though we address the replicability problem in behavioral genetics, our strategy is also applicable in other areas where concern about replicability has been raised. across-laboratory replicability | mixed-model ANOVA | open-field behavior
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- 2005
11. Inactivation of tensin3 in mice results in growth retardation and postnatal lethality
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Chiang, Ming-Ko, Liao, Yi-Chun, Kuwabara, Yasuko, and Lo, Su Hao
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Growth -- Research ,Growth -- Genetic aspects ,Proteins -- Research ,Mice as laboratory animals -- Research ,Developmental biology -- Research ,Biological sciences - Abstract
Tensin family is a group of focal adhesion proteins that interact with integrins, actin, and phosphotyrosine-containing proteins. To explore the in vivo functions of a new member of the family, tensin3, we have generated mutant mice with a disrupted tensin3 gene. Inactivation of tensin3 resulted in growth retardation and postnatal lethality in one third of the homozygous mutants. Histological analysis of those mutants showed incomplete development of the small intestine, lung, and bone. Villus formation in the small intestine was affected and cells migrated slower in the runt mutants. Their lungs also displayed enlarged air space suggesting defects in alveogenesis. In addition, the resting zone was thicker and fewer proliferating cells were present in the growth plates of tensin[3.sup.-/-] tibiae. These observations indicate that tensin3 is essential for normal development and functions of the small intestine, lung, and bone. These phenotypes of the runt tensin[3.sup.-/-] mice are similar to some clinical features of Silver-Russell syndrome (SRS) which is a genetically inherited defect. About 10% of SRS cases have been linked to abnormality in chromosome 7p11.2-13, where human tensin3 gene is located, suggesting a potential link between tensin3 and SRS. Keywords: Tensin; Focal adhesion; Growth retardation; Postnatal lethality; Alveogenesis; Growth plates; Silver-Russell syndrome
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- 2005
12. Pregnane X receptor prevents hepatorenal toxicity from cholesterol metabolites
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Sonoda, Junichiro, Chong, Ling Wa, Downes, Michael, Barish, Grant D., Coulter, Sally, Liddle, Christopher, Lee, Chih-Hao, and Evans, Ronald M.
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Metabolites -- Research ,Mice as laboratory animals -- Research ,Cholesterol metabolism -- Research ,Cardiovascular diseases -- Causes of ,Science and technology - Abstract
Efficient detoxification and clearance of cholesterol metabolites such as oxysterols, bile alcohols, and bile acids are critical for survival because they can promote liver and cardiovascular disease. We report here that loss of the nuclear xenobiotic receptor PXR (pregnane X receptor), a regulator of enterohepatic drug metabolism and clearance, results in an unexpected acute lethality associated with signs of severe hepatorenal failure when mice are fed with a diet that elicits accumulation of cholesterol and its metabolites. Induction of a distinct drug clearance program by a high-affinity ligand for the related nuclear receptor, the constitutive androstane receptor, does not overcome the lethality, indicating the unique requirement of PXR for detoxification. We propose that the PXR signaling pathway protects the body from toxic dietary cholesterol metabolites, and, by extension, PXR ligands may ameliorate human diseases such as cholestatic liver diseases and the associating acute renal failure.
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- 2005
13. Strain-specified characteristics of mouse synthetic prions
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Legname, Giuseppe, Nguyen, Hoang-Oanh B., Baskakov, Ilia V., Cohen, Fred E., DeArmond, Stephen J., and Prusiner, Stanley B.
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Prions -- Research ,Mice as laboratory animals -- Research ,Nervous system -- Degeneration ,Nervous system -- Research ,Science and technology - Abstract
Synthetic prions were produced in our laboratory by using recombinant mouse prion protein (MoPrP) composed of residues 89-230. The first mouse synthetic prion strain (MoSP1) was inoculated into transgenic (Tg) 9949 mice expressing N-terminally truncated MoPrP([DELTA]23-88) and WT FVB mice expressing full-length MoPrP. On first and second passage in Tg9949 mice, MoSP1 prions caused disease in 516 [+ or -] 27 and 258 [+ or -] 25 days, respectively; numerous, large vacuoles were found in the brainstem and gray matter of the cerebellum. MoSP1 prions passaged in Tg9949 mice were inoculated into FVB mice; on first and second passage, the FVB mice exhibited incubation times of 154 [+ or -] 4 and 130 [+ or -] 3 days, respectively. In FVB mice, vacuolation was less intense but more widely distributed, with numerous lesions in the hippocampus and cerebellar white matter. This constellation of widespread neuropathologic changes was similar to that found in FVB mice inoculated with Rocky Mountain Laboratory (RML) prions, a strain derived from a sheep with scrapie. Conformational stability studies showed that the half-maximal GdnHCl ([Gdn.sub.1/2]) concentration for denaturation of MoSP1 prions passaged in Tg9949 mice was [approximately equal to] 4.2 M; passage in FVB mice reduced the [Gdn.sub.1/2] value to [approximately equal to] 1.7 M. RML prions passaged in either Tg9949 or FVB mice exhibited [Gdn.sub.1/2] values of [approximately equal to]1.8 M. The incubation times, neuropathological lesion profiles, and [Gdn.sub.1/2] values indicate that MoSP1 prions differ from RML and many other prion strains derived from sheep with scrapie and cattle with bovine spongiform encephalopathy. neurodegeneration
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- 2005
14. Parkin-deficient mice are not a robust model of parkinsonism
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Perez, Francisco A. and Palmiter, Richard D.
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Parkinson's disease -- Causes of ,Parkinson's disease -- Research ,Mice as laboratory animals -- Research ,Animal mutation -- Research ,Genetic research ,Science and technology - Abstract
Mutations in the human parkin gene cause autosomal recessive juvenile parkinsonism, a heritable form of Parkinson's disease (PD). To determine whether mutations in the mouse parkin gene (Park2) also result in a parkinsonian phenotype, we generated mice with a targeted deletion of parkin exon 2. Using an extensive behavioral screen, we evaluated neurological function, motor ability, emotionality, learning, and memory in aged Parkin-deficient mice. The behavioral profile of Parkin-deficient mice on a B6;129S4 genetic background was strikingly similar to that of control mice, and most differences were not reproducible by using coisogenic mice on a 129S4 genetic background. Moreover, catecholamine levels in the striatum, olfactory bulb, and spinal cord of Parkin-deficient mice were normal. In contrast to previous studies using independently generated Parkin-deficient mice, we found no evidence for nigrostriatal, cognitive, or noradrenergic dysfunction. Understanding why Parkin-deficient mice do not exhibit robust signs of parkinsonism could advance knowledge and treatment of PD. mouse behavior | dopamine | norepinephrine | gene knockout | Parkinson
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- 2005
15. A targeted mutation in Cacng4 exacerbates spike-wave seizures in stargazer (Cacng2) mice
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Letts, Verity A., Mahaffey, Connie L., Beyer, Barbara, and Frankel, Wayne N.
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Mice as laboratory animals -- Research ,Calcium channels -- Research ,Animal mutation -- Research ,Science and technology - Abstract
The voltage-dependent calcium channel [gamma]4 subunit protein, CACNG4, is closely related to the [gamma]2 subunit, CACNG2. Both are expressed primarily in the brain and share 53% amino acid identity. The Cacng2 gene is disrupted in the stargazer mouse, with its distinctive phenotype including ataxia, frequent absence seizure episodes, and head elevation. A disruption within Cacng4 was engineered to assess its particular function. The homozygous Cacng4-targeted mutant mouse appeared normal with no ataxic gait or absence seizures, suggesting that other members of the T subunit family might functionally compensate for the absence of CACNG4. To test this hypothesis, the targeted Cacng4 mutation was combined with alleles of Cacng2. Absence seizures were observed in combination with the stargazer 3J mutation, which itself does not have seizures, and increased seizure activity was observed in combination with the waggler allele. Furthermore, within the corticothalamic loop, where absence seizures arise, CACNG4 expression is restricted to the thalamus. Our studies show that the CACNG4 protein has seizure suppressing activity, but this effect is revealed only when CACNG2 expression is also compromised, suggesting that CACNG subunits have in vivo overlapping functions. stargazer mutants | absence epilepsy | [gamma]4 expression
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- 2005
16. Overexpression of PCSK9 accelerates the degradation of the LDLR in a post-endoplasmic reticulum compartment
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Maxwell, Kara N., Fisher, Edward A., and Breslow, Jan L.
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Proteases -- Research ,Low density lipoprotein receptors -- Research ,Mice as laboratory animals -- Research ,Hypercholesterolemia -- Research ,Science and technology - Abstract
Proprotein convertase subtilisin kexin 9 (PCSK9) is a member of the subtilisin serine protease family with an important role in cholesterol metabolism. PCSK9 expression is regulated by dietary cholesterol in mice and cellular sterol levels in cell culture via the sterol regulatory element binding protein transcription factors, and mutations in PCSK9 are associated with a form of autosomal dominant hypercholesterolemia. Overexpression of PCSK9 in mice leads to increased total and low-density lipoprotein (LDL) cholesterol levels because of a decrease in hepatic LDL receptor (LDLR) protein with normal mRNA levels. To study the mechanism, PCSK9 was overexpressed in human hepatoma cells, HepG2, by adenovirus. Overexpression of PCSK9 in HepG2 cells caused a decrease in whole-cell and cell-surface LDLR levels. PCSK9 overexpression had no effect on LDLR synthesis but caused a dramatic increase in the degradation of the mature LDLR and a lesser increase in the degradation of the precursor LDLR. In contrast, overexpression of a catalytically inactive mutant PCSK9 prevented the degradation of the mature LDLR; whereas increased degradation of the precursor LDLR still occurred. The PCSK9-induced degradation of the LDLR was not affected by inhibitors of the proteasome, lysosomal cysteine proteases, aspartic acid proteases, or metalloproteases. The PCSK9induced degradation of the LDLR was shown to require transport out of the endoplasmic reticulum. These results indicate that overexpression of PCSK9 induces the degradation of the LDLR by a nonproteasomal mechanism in a post-endoplasmic reticulum compartment. autosomal dominant hypercholesterolemia | Narc-1 | proprotein convertase subtilisin kexin 9 | low-density lipoprotein receptor
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- 2005
17. Nuclear receptors constitutive androstane receptor and pregnane X receptor ameliorate cholestatic liver injury
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Stedman, Catherine A.M., Liddle, Cristopher, Coulter, Sally A., Sonoda, Junichiro, Alvarez, Jacqueline G.A., Moore, David D., Evans, Ronald M., and Downes, Michael
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Cholestasis -- Research ,Cholestasis -- Care and treatment ,Jaundice, Obstructive -- Research ,Jaundice, Obstructive -- Care and treatment ,Bile acids -- Research ,Liver diseases -- Research ,Liver diseases -- Causes of ,Mice as laboratory animals -- Research ,Science and technology - Abstract
Cholestasis is associated with accumulation of bile acids and lipids, and liver injury. The constitutive androstane receptor (CAR) and pregnane X receptor (PXR) are xenobiotic nuclear receptors that coordinate protective hepatic responses to potentially toxic stimuli, including bile acids. We investigated the role of these receptors in the regulation of bile acid and lipid metabolism in a bile duct ligation (BDL) model of cholestasis applied to receptor knockout mice. Hepatic damage from bile acid accumulation was increased in both CAR knockout (CARKO) and PXR knockout mice, but bile acid concentrations were lower in CARKO mice. High-density lipoprotein (HDL) cholesterol was elevated in CARKO mice, and serum total cholesterol increased less in CARKO or PXR knockout mice than WT mice after BDL. Gene expression analysis of the BDL knockout animals demonstrated that, in response to cholestasis, PXR and CAR both repressed and induced the specific hepatic membrane transporters Oatp-c (organic anion transporting polypeptide C) and Oatp2 ([Na.sup.+]-dependent organic anion transporter 2), respectively. Induction of the xenobiotic transporter multidrug resistance protein 1 in cholestasis was independent of either PXR or CAR, in contrast to the known pattern of induction of multidrug resistance protein 1 by xenobiotics. These results demonstrate that CAR and PXR influence cholesterol metabolism and bile acid synthesis, as well as multiple detoxification pathways, and suggest their potential role as therapeutic targets for the treatment of cholestasis and lipid disorders. bile acid | cholestasis | high-density lipoprotein | cholesterol
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- 2005
18. Liver receptor homolog 1 contributes to intestinal tumor formation through effects on cell cycle and inflammation
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Schoonjans, Kristina, Dubuquoy, Laurent, Mebis, Joseph, Fayard, Elisabeth, Wendling, Olivia, Haby, Celine, Geboes, Karel, and Auwerx, Johan
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Mice as laboratory animals -- Research ,Colorectal diseases -- Research ,Colon cancer -- Research ,Gastrointestinal diseases -- Research ,Cell cycle ,Science and technology - Abstract
Liver receptor homolog 1 (LRH-1) is an orphan nuclear receptor that synergizes with [beta]-catenin/T cell factor 4 signaling to stimulate intestinal crypt cell renewal. We evaluated here the impact of haploinsufficiency of LRH-1 on intestinal tumorigenesis by using two independent mouse models of human colon tumorigenesis. Haploinsufficiency of LRH-1 blunts intestinal tumorigenesis in the [Apc.sup.Min/+] mice, a genetic model of intestinal cancer. Likewise, [Lrh-1.sup.+/-] mice are protected against the formation of aberrant crypt foci in the colon of mice exposed to the carcinogen azoxymethane. LRH-1 gene expression is reduced in tumors that express elevated levels of the proinflammatory cytokine TNF-[alpha] Reciprocally, decreased LRH-1 expression in [Lrh-1.sup.+/-] mice attenuates TNF-[alpha] expression. Compared with normal human colon, expression and subcellular localization of LRH-1 is significantly altered in neoplastic colon. In combination, these data suggest a role of LRH-1 in the initiation of intestinal tumorigenesis both by affecting cell cycle control as well as through its impact on inflammatory pathways. [beta]-catenin | colon cancer | nuclear receptors
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- 2005
19. Mapping quantitative trait loci for anxiety in chromosome substitution strains of mice
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Singer, Jonathan B., Hill, Annie E., Nadeau, Joseph H., and Lander, Eric S.
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Mice as laboratory animals -- Research ,Quantitative trait loci ,Genetic variation ,Chromosomes ,Genetic research ,Biological sciences - Abstract
Anxious behavior in the mouse is a complex quantitative phenotype that varies widely among inbred mouse strains. We examined a panel of chromosome substitution strains bearing individual A/J chromosomes in an otherwise C57BL/6J background in open-field and light-dark transition tests. Our results confirmed previous reports of quantitative trait loci (QTL) on chromosomes 1, 4, and 15 and identified novel loci on chromosomes 6 and 17. The studies were replicated in two separate laboratories. Systematic differences in the overall activity level were found between the two facilities, but the presence of the QTL was confirmed in both laboratories. We also identified specific effects on open-field defecation and center avoidance and distinguished them from overall open-field activity.
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- 2005
20. Genetic A[T.sub.1A] receptor deficiency attenuates cold-induced hypertension
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Sun, Zhongjie, Wang, Xiuqing, Wood, Charles E., and Cade, J. Robert
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Mice as laboratory animals -- Research ,Hypertrophy -- Research ,Blood pressure -- Research ,Biological sciences - Abstract
The aim of this study was to test our hypotheses that A[T.sub.1A] receptors play a role in the pathogenesis of cold-induced hypertension (CIH) and in the cold-induced increase in drinking responses to ANG II. Two groups of wild-type (WT) and two groups of A[T.sub.1A] receptor gene knockout (A[T.sub.1A]-KO) mice were used (6/ group). Blood pressures (BP) of the four groups were similar during the control period at room temperature (25[degrees]C). After the control period, one group of WT and one group of A[T.sub.1A]-KO mice were exposed to cold (5[degrees]C), while the remaining groups were kept at 25[degrees]C. BP of the cold-exposed WT group elevated significantly within 1 wk of exposure to cold and increased gradually to a maximum level by week 5. However, there was only a slight increase in BP of the cold-exposed A[T.sub.1A]-KO group. The maximal cold-induced increase in BP ([DELTA]BP) is significantly less in A[T.sub.1A]-KO group (11 [+ or -] 3 mmHg) than in WT group (49 [+ or -] 6 mmHg), indicating that A[T.sub.1A] receptor deficiency attenuates cold-induced elevation of BP. Interestingly, both WT and A[T.sub.1A]-KO mice developed cardiac and renal hypertrophy to the same extent. A[T.sub.1A]-KO caused a significant increase in urine and plasma levels of nitric oxide (NO), indicating that the renin-angiotensin system inhibits NO formation probably via A[T.sub.1A] receptors. Cold exposure inhibited endothelial NO synthase protein expressions and decreased urine and plasma levels of NO, which may be mediated partially by A[T.sub.1A] receptors. A[T.sub.1A]-KO completely abolished the cold-induced increase in drinking responses to ANG II. We conclude that 1) A[T.sub.1A] receptors play an essential role in the pathogenesis of CIH but not cardiac hypertrophy; 2) the role of A[T.sub.1A] receptors in CIH may be mediated partially by its inhibitory effect on the NO system; and 3) cold-induced increase in drinking response to ANG II is mediated by A[T.sub.1A] receptors. blood pressure; cardiac hypertrophy; mice; nitric oxide; endothelial nitric oxide synthase
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- 2005
21. Requirement for serum response factor for skeletal muscle growth and maturation revealed by tissue-specific gene deletion in mice
- Author
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Li, Shijie, Czubryt, Michael P., McAnally, John, Bassel-Duby, Rhonda, Richardson, James A., Wiebel, Franziska F., Nordheim, Alfred, and Olson, Eric N.
- Subjects
Muscle diseases -- Research ,Mice as laboratory animals -- Research ,Science and technology - Abstract
Serum response factor (SRF) controls the transcription of muscle genes by recruiting a variety of partner proteins, including members of the myocardin family of transcriptional coactivators. Mice lacking SRF fail to form mesoderm and die before gastrulation, precluding an analysis of the roles of SRF in muscle tissues. To investigate the functions of SRF in skeletal muscle development, we conditionally deleted the Srf gene in mice by skeletal muscle-specific expression of Cre recombinase. In mice lacking skeletal muscle SRF expression, muscle fibers formed, but failed to undergo hypertrophic growth after birth. Consequently, mutant mice died during the perinatal period from severe skeletal muscle hypoplasia. The myopathic phenotype of these mutant mice resembled that of mice expressing a dominant negative mutant of a myocardin family member in skeletal muscle. These findings reveal an essential role for the partnership of SRF and myocardin-related transcription factors in the control of skeletal muscle growth and maturation in vivo. hypertrophy | myocardin-related transcription factor | myofiber | myopathy
- Published
- 2005
22. Expression profile of a human inducible nitric oxide synthase promoter reporter in transgenic mice during endotoxemia
- Author
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Yu, Zhiyuan, Xia, Xuefeng, and Kone, Bruce C.
- Subjects
Mice as laboratory animals -- Research ,Bacteremia -- Research ,Septic shock ,Polysaccharides ,Nitric oxide ,Acute renal failure ,Biological sciences - Abstract
Inducible nitric oxide synthase (iNOS) is involved in many physiological and pathophysiological processes, including septic shock and acute kidney failure. Little is known about transcriptional regulation of the human iNOS gene in vivo under basal conditions or in sepsis. Accordingly, we developed transgenic mice carrying an insertional human iNOS promoter-reporter gene construct. In these mice, the proximal 8.3 kb of the human iNOS 5'-flanking region controls expression of the reporter gene of enhanced green fluorescent protein (EGFP). Patterns of human iNOS promoter/EGFP transgene expression in tissues were examined by fluorescence microscopy and immunoblotting. Endogenous murine iNOS was basally undetectable in kidney, intestine, spleen, heart, lung, liver, stomach, or brain. In contrast, EGFP from the transgene was basally expressed in kidney, brain, and spleen, but not the other tissues of the transgenic mice. Bacterial lipopolysaccharide induced endogenous iNOS expression in kidney, intestine, spleen, lung, liver, stomach, and heart, but not brain. In contrast, human iNOS promoter/EGFP transgene expression was induced above basal levels only in intestine, spleen, brain, stomach, and lung. Within kidney, human iNOS promoter/EGFP fluorescence was detected most prominently in proximal tubules of the outer cortex and collecting ducts and colocalized with endogenous mouse iNOS. Within the collecting duct, both endogenous iNOS and the human iNOS promoter/EGFP transgene were expressed in cells lacking aquaporin-2 immunoreactivity, consistent with expression in intercalated cells. Although it remains possible that essential regulatory elements reside in remote locations of the gene, our data concerning this 8.3-kb region provide the first in vivo evidence suggesting differential transcriptional control of the human iNOS gene in these organs and marked differences in transcriptional regulatory regions between the murine and human genes. green fluorescent protein: gene transcription: lipopolysaccharide: kidney
- Published
- 2005
23. Enhancer-dependent inhibition of mouse renin transcription by inflammatory cytokines
- Author
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Pan, Li, Wang, Yanping, Jones, Craig A., Glenn, Sean T., Baumann, Heinz, and Gross, Kenneth W.
- Subjects
Mice as laboratory animals -- Research ,Renin ,Cytokines ,Biological sciences - Abstract
Inflammatory cytokines have been shown to inhibit renin gene expression in the kidney in vivo and the kidney tumor-derived As4.1 cell line. In this report, we show that cytokines oncostatin M (OSM), IL-6, and IL-113 inhibit transcriptional activity associated with 4.1 kb of the mouse renin 5'-flanking sequence in As4.1 cells. The 242-bp enhancer (-2866 to -2625 bp) is sufficient to mediate the observed inhibitory effects. Sequences within the enhancer required for inhibition by each of these cytokines have been determined by deletional and mutational analysis. Results indicate that a 39-bp region (CEC) containing a cAMP-responsive element, an E-box, and a steroid receptor-binding site, previously identified as the most critical elements for enhancer activity, is sufficient for the inhibition induced by IL-1[beta]. However, mutation of each of the three component sites does not abolish the inhibition by IL-1[beta], suggesting that the target(s) of cytokine action may not be the transcription factors binding directly to these sites. This CEC region is also critical, but not sufficient, for the inhibition mediated by OSM and IL-6. These data suggest that the direct target of the associated cytokines may be coactivators interacting with transcription factors binding at the enhancer. Finally, we show that OSM treatment caused a 17-fold increase in promoter activity when only 2,625 bp of the Ren-[1.sup.c] flanking sequence were tested, in which the enhancer is not present. Three regions including -2625 to - 1217 bp, the HOX*PBX binding site at -60 bp, and -59 to +6 bp have been found to contribute to this induction. As4.1; oncostatin M; IL-6; IL-1[beta]
- Published
- 2005
24. A novel pathological role of p53 in kidney development revealed by gene-environment interactions
- Author
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Fan, Hao, Harrell, Jessica R., Dipp, Susana, Saifudeen, Zubaida, and El-Dahr, Samir S.
- Subjects
Neuropeptides -- Research ,Mice as laboratory animals -- Research ,Histones ,Bradykinin ,Biological sciences - Abstract
Gene-environment interactions are implicated in congenital human disorders. Accordingly, there is a pressing need to develop animal models of human disease, which are the product of defined gene-environment interactions. Previously, our laboratory demonstrated that gestational salt stress of bradykinin [B.sub.2] receptor ([B.sub.2]R)-null mice induces renal dysgenesis and early death of the offspring (E1-Dahr SS, Harrison-Bernard LM, Dipp S, Yosipiv IV, and Meleg-Smith S. Physiol Genomics 3: 121-131, 2000). In contrast, salt-stressed [B.sub.2]R +/+ or +/- littermates have normal development. The present study investigates the mechanisms underlying the susceptibility of B2R-null mice to renal dysgenesis. Proteomic and conventional Western blot screens identified E-cadherin among the differentially repressed proteins in [B.sub.2]R-/- kidneys, whereas the checkpoint kinase Chkl and its substrate P-[Ser.sup.20] p53 were induced. We tested the hypothesis that p53 mediates repression of E-cadherin gene expression and is causally linked to the renal dysgenesis. Genetic crosses between [B.sub.2]R -/- and p53+/- mice revealed that germline reduction of p53 gene dosage rescues [B.sub.2]R-/- mice from renal dysgenesis and restores kidney E-cadherin gene expression. Furthermore, [gamma]-irradiation induces repression of E-cadherin gene expression in p53+/+ but not -/- cells. In transient transfection assays, p53 repressed human E-cadherin promoter-driven reporter activity, whereas a mutant p53, which cannot bind DNA, did not. Functional promoter analysis indicated the presence of a p53-responsive element in exon 1, which partially mediates p53-induced repression. Chromatin immunoprecipitation assays revealed that p53 inhibits histone acetylation of the E-cadherin promoter. Treatment with a histone deacetylase inhibitor reversed both p53-mediated promoter repression and deacetylation. In conclusion, this study demonstrates that gene-environment interactions cooperate to induce congenital defects through p53 activation. bradykinin [B.sub.2] receptor: knockout mice; checkpoint kinase; histone acetylation
- Published
- 2005
25. The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice
- Author
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Nagashima, Kei, Matsue, Kenta, Konishi, Masahiro, Iidaka, Chisato, Miyazaki, Koyomi, Ishida, Norio, and Kanosue, Kazuyuki
- Subjects
Mice as laboratory animals -- Research ,Circadian rhythms -- Effect of chemicals on ,Metabolism ,Body temperature ,Biological rhythms ,Biological sciences - Abstract
The criptochrome genes (Cry1 and Cry2) are involved in the molecular mechanism that controls the circadian clock, and mice lacking these genes ([Cry1.sup.-/-]/ [Cry2.sup.-/-]) are behaviorally arrhythmic. It has been speculated that the circadian clock modulates the characteristics of thermoregulation, resulting in body temperature ([T.sub.b]) rhythm. However, there is no direct evidence proving this speculation. We show here that Tb and heat production in [Cry1.sup.-/-]/[Cry2.sup.-/-] mice are arrhythmic under constant darkness. In contrast, both rhythms occur under a light-dark cycle and/or periodical food restriction linked with spontaneous activity and/or eating, although they are not robust as those in wild-type mice. The relationship between heat production and [T.sub.b] in [Cry1.sup.-/-]/[Cry2.sup.-/-] mice is linear and identical under any conditions, indicating that their [T.sub.b] rhythm is determined by heat production rhythm associated with activity and eating. However, [T.sub.b] in wild-type mice is maintained at a relatively higher level in the active phase than the inactive phase regardless of the heat production level. These results indicate that the thermoregulatory responses are modulated according to the circadian phase, and the Cry genes are involved in this mechanism. circadian clock; thermoregulation; metabolism
- Published
- 2005
26. Dietary copper deficiency reduces iron absorption and duodenal enterocyte hephaestin protein in male and female rats
- Author
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Reeves, Philip G., DeMars, Lana C.S., Johnson, W. Thomas, and Lukaski, Henry C.
- Subjects
Copper in the body -- Health aspects ,Iron in the body -- Health aspects ,Mice as laboratory animals -- Research ,Food/cooking/nutrition - Abstract
The mechanism for reduced Fe absorption in Cu deficiency is unknown, but may involve the intestinal Cu-dependent ferroxidase, Hephaestin (Hp). A 2 x 2 factorial experiment was designed to include Cu-deficient (CUD) and Cu-adequate (CuA) male and female rats. Weanling rats of both sexes were randomly divided into 2 groups each and fed an AIN-93G diet with tow (< 0.3 mg/kg; CuD) or adequate Cu (5.0 mg/kg; CuA). After 19 d, rats were fed 1.0 g each of their respective diets labeled with [sup.59]Fe. Retained [sup.59]Fe was monitored by whole-body counting for 12 d. Then, rats were killed for [sup.59]Fe and Fe measurements in blood and various organs. Duodenal enterocytes were isolated for Western blot analysis of Hp. Signs of Cu and Fe deficiency were evident in both sexes. CuD male rats absorbed 60% as much Fe as CuA male rats (P < 0.001), whereas CuD female rats absorbed 70% (P < 0.001) as much as CuA females, with no difference between the sexes. Hp protein in enterocytes of CuD rats of both sexes was only 35% of that in CuA rats. The biological half-life of [sup.59]Fe in CuD rats was only 50% (P < 0.001) of that in CuA rats, suggesting that Fe turnover was faster in CuD rats than CuA rats. Serum, spleen, and kidney Fe were lower (P < 0.001) in CuD rats than in CuA rats. Duodenal mucosa and liver Fe were higher (P < 0.01) in CuD male rats than CuA rats. Duodenal Fe but not liver Fe was higher in CuD female rats than CuA rats. Liver Fe was much higher ( KEY WORDS: * copper deficiency * iron * absorption * biological half-life * rats
- Published
- 2005
27. Metallothionein induction is not involved in cadmium accumulation in the duodenum of mice and rats fed diets containing high-cadmium rice or sunflower kernels and a marginal supply of zinc, iron, and calcium
- Author
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Reeves, Philip G., Chaney, Rufus L., Simmons, Robert W., and Cherian, M. George
- Subjects
Diet therapy -- Research ,Metallothionein -- Health aspects ,Mice as laboratory animals -- Research ,Cadmium -- Health aspects ,Food/cooking/nutrition - Abstract
Rats fed diets with cadmium (Cd) concentrations similar to that found in human diets, and nutritionally marginal with respect to iron (Fe), zinc (Zn), and calcium (Ca) retained 10 times more Cd in the duodenum than rats fed adequate mineral diets. In the current study, 2 experiments were performed to determine the role of intestinal metallothionein (MT) in the accumulation of duodenal Cd, and to determine whether endogenous rice grain Cd is as available as Cd exogenously incorporated into the grain. In Expt. 1, wild-type and MT-null mice were fed 40% rice diets containing marginal or adequate amounts of Fe, Zn, and Ca, and 240 [micro]g Cd/kg. Duodenal Cd was 10 times higher in both wild-type and MT-null mice regardless of their mineral status. In Expt. 2, one group of rats was fed 40% rice diets in which Cd was incorporated into the rice during growth and maturation, and another group was fed 40% rice diets in which Cd was incorporated into the rice during cooking. Each group also was fed either marginal or adequate amounts of Zn, Fe, and Ca. After 5 wk, rats were given a single meal labeled with [sup.109]Cd, and the amount of label retained after 7 d was determined by whole-body counting. Rats with marginal mineral status retained 10 times more [sup.109]Cd than those with adequate status; however, there was no difference between rats fed endogenous or exogenous Cd rice. Although duodenal Cd concentration was 8 times higher in the marginally fed rats, MT concentration was unchanged. These 2 experiments indicate that MT induction is not involved in duodenal Cd accumulation in animals with marginal dietary status of Fe, Zn, and Ca. In addition, they support the hypothesis that marginal deficiencies of Fe, Zn, and Ca, commonly found in certain human populations subsisting on rice-based diets, play an important role in increasing the risk of dietary Cd exposure. KEY WORDS: * cadmium * metallothionein * MT-null mice * rats * rice
- Published
- 2005
28. Dietary conjugated linoleic acid alleviates nonalcoholic fatty liver disease in Zucker (fa/fa) rats
- Author
-
Nagao, Koji, Inoue, Nao, Wang, Yu-Ming, Shirouchi, Bungo, and Yanagita, Teruyoshi
- Subjects
Mice as laboratory animals -- Research ,Liver diseases -- Diet therapy ,Linoleic acids -- Health aspects ,Food/cooking/nutrition - Abstract
Nonalcoholic fatty liver disease (NAFLD) is the preferred term to describe the spectrum of liver damage ranging from hepatic steatosis to steatohepatitis, liver fibrosis, and cirrhosis, and it is emerging as the most common liver disease in industrialized countries. Thus, the discovery of food components that would ameliorate NAFLD is of interest. Conjugated linoleic acid (CLA), a mixture of positional and geometric isomers of linoleic acid, has attracted considerable attention because of its potentially beneficial biological effects both in vitro and in vivo. We tested whether dietary CLA protects Zucker (fa/fa) rats from hepatic injury. After 8 wk of feeding, hepatomegaly, hepatic triglyceride (TG) accumulation, and elevated hepatic injury markers in plasma were markedly alleviated in CLA-fed Zucker rats compared with linoleic acid-fed (control) rats. These effects were attributed in part to the enhanced hepatic activities of carnitine palmitoyltransferase, a key enzyme of fatty acid [beta]-oxidation, and microsomal TG transfer protein, an important factor for lipoprotein secretion due to the CLA diet. We previously reported that the severe hyperinsulinemia in control Zucker rats was attenuated in CLA-fed rats due to an enhanced level of plasma adiponectin, which improves insulin sensitivity. In the present study, the adiponectin concentration was increased and the mRNA expression of tumor necrosis factor-[alpha], an inflammatory cytokine, was markedly suppressed in the liver of CLA-fed Zucker rats. We speculate that the enhanced level of liver adiponectin may prevent the development and progression of NAFLD in CLA-fed Zucker rats. KEY WORDS: * conjugated linoleic acid * nonalcoholic fatty liver disease * adiponectin * tumor necrosis factor-[alpha] * Zucker (fa/fa) rats
- Published
- 2005
29. RHAMM, a receptor for hyaluronan-mediated motility, compensates for CD44 in inflamed CD44-knockout mice: a different interpretation of redundancy
- Author
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Nedvetzki, Shlomo, Gonen, Erez, Assayag, Nathalie, Reich, Reuven, Williams, Richard O., Thurmond, Robin L., Huang, Jing-Feng, Neudecker, Birgit A., Wang, Fu-Shang, Turley, Eva A., and Naor, David
- Subjects
Mice as laboratory animals -- Research ,Genes -- Research ,Hyaluronic acid ,Science and technology - Abstract
We report here that joint inflammation in collagen-induced arthritis is more aggravated in CD44-knockout mice than in WT mice, and we provide evidence for molecular redundancy as a causal factor. Furthermore, we show that under the inflammatory cascade, RHAMM (receptor for hyaluronan-mediated motility), a hyaluronan receptor distinct from CD44, compensates for the loss of CD44 in binding hyaluronic acid, supporting cell migration, up-regulating genes involved with inflammation (as assessed by microarrays containing 13,000 cDNA clones), and exacerbating collagen-induced arthritis. Interestingly, we further found that the compensation for loss of the CD44 gene does not occur because of enhanced expression of the redundant gene (RHAMM), but rather because the loss of CD44 allows increased accumulation of the hyaluronic acid substrate, with which both CD44 and RHAMM engage, thus enabling augmented signaling through RHAMM. This model enlightens several aspects of molecular redundancy, which is widely discussed in many scientific circles, but the processes are still ill defined.
- Published
- 2004
30. Effects of aqueous extracts of 'betel quid' and its constituents on testosterone production by dispersed mouse interstitial cells
- Author
-
Nai-Yen Jack Yang, Khrishna Kaphle, Pei-Hwa Wang, De-Shien Jong, Leang-Shin Wu, and Jen-Hsou Lin
- Subjects
Medicine, Chinese -- Research ,Testosterone -- Research ,Materia medica, Vegetable -- Research ,Materia medica, Vegetable -- Health aspects ,Plant extracts -- Research ,Plant extracts -- Health aspects ,Mice as laboratory animals -- Research ,Medicine, Botanic -- Research ,Medicine, Herbal -- Research ,Chewing gum -- Health aspects ,Chewing gum -- Research ,Health - Published
- 2004
31. Anatomic and energetic correlates of divergent selection for basal metabolic rate in laboratory mice
- Author
-
Ksiazek, Aneta, Konarzewski, Marek, and Lapo, Iwona B.
- Subjects
Basal metabolism -- Research ,Basal metabolism -- Physiological aspects ,Mice as laboratory animals -- Research ,Mice as laboratory animals -- Physiological aspects ,Biological sciences ,Zoology and wildlife conservation - Published
- 2004
32. Inhibition of hedgehog signaling protects adult mice from diet-induced weight gain
- Author
-
Buhman, Kimberly K., Wang, Li Chun, Tang, Yuzhu, Swietlicki, Elzbieta A., Kennedy, Susan, Xie, Yan, Liu, Zhong-Yi, Burkly, Linda C., Levin, Marc S., Rubin, Deborah C., and Davidson, Nicholas O.
- Subjects
Mice as laboratory animals -- Food and nutrition ,Mice as laboratory animals -- Health aspects ,Mice as laboratory animals -- Research ,Food/cooking/nutrition - Abstract
Hedgehog (Hh) signaling plays an important role in embryonic development of many tissues, including the gastrointestinal tract. Sonic Hh-and Indian Hh-deficient mice die before or soon after birth, precluding further study of this signaling pathway in the mature intestine. Maternal transfer of inactivating monoclonal antibodies to Hh proteins (anti-Hh moAb) during late stages of embryogenesis or to early postnatal mice produced intestinal villous abnormalities, progressive runting, and severe malabsorption of dietary fat. In the present study, we sought to determine the effect of inhibiting Hh signaling on weight gain and lipid absorption in adult mice. Anti-Hh moAb was administered to adult Balb/c mice fed either a low-fat, nonpurified diet or a high-fat, semipurified diet, and to adult ob/ob mice fed the low-fat, nonpurified diet. Weight gain was significantly inhibited by anti-Hh moAb treatment in Balb/C mice fed the high-fat, but not the low-fat diet and in ob/ob mice. Further analysis of adult Balb/c mice fed the high-fat diet demonstrated that although total lipid absorption was normal, the rate of triglyceride absorption was significantly delayed in mice treated with anti-Hh moAb and they had significantly increased fecal FFA excretion. Hepatic steatosis, found in high-fat fed Balb/c mice treated with the control moAb, was abrogated by anti-Hh moAb administration. These findings point to a potential role for Hh signaling pathways in diet-induced abnormalities of lipid metabolism. J. Nutr. 134: 2979-2984, 2004. KEY WORDS: * hedgehog signaling * intestinal triglyceride absorption * hepatic steatosis
- Published
- 2004
33. Mice deficient in methylenetetrahydrofolate reductase exhibit tissue-specific distribution of folates
- Author
-
Ghandour, Haifa, Chen, Zhoutao, Selhub, Jacob, and Rozen, Rima
- Subjects
Mice as laboratory animals -- Food and nutrition ,Mice as laboratory animals -- Research ,Food/cooking/nutrition - Abstract
Methylenetetrahydrofolate reductase (MTHFR) catalyzes the synthesis of 5-methyltetrahydrofolate (5-methylTHF), which is used for homocysteine remethylation to methionine, the precursor of S-adenosylmethionine (SAM). Impairment of MTHFR will increase homocysteine levels and compromise SAM-dependent methylation reactions. Mild MTHFR deficiency is common in many populations due to a polymorphism at bp 677. To assess how impaired MTHFR activity affects folate metabolism in various tissues in vivo, we used affinity/HPLC with electrochemical detection to analyze the distribution of folates in plasma, liver, and brain of Mthfr-deficient mice. The most pronounced difference in total folate was observed in plasma. In Mthfr -/- mice, plasma total folate levels, were ~25% of those in wild-type (Mthfr +/+) mice. Only 40% of plasma folate in Mthfr -/- mice was comprised of 5-methylTHF, compared with at least 80% in the other 2 genotype groups. In liver and brain, there were no differences in total folate. However, the proportion of 5-methylTHF in both tissues was again markedly reduced in mice with the Mthfr -/- genotype. In this genotype group, 5-methylTHF is likely derived from the diet. Our study demonstrated reduced total circulatory folate and altered distribution of folate derivatives in liver and brain in Mthfr deficiency. Decreased methylfolates and increased nonmethylfolates would affect the flux of one-carbon units between methylation reactions and nucleotide synthesis. This altered flux has implications for several common disorders, including cancer and vascular disease. J. Nutr. 134: 2975-2978, 2004. KEY WORDS: * folate distribution * methyltetrahydrofolate * formylated-folates * MTHFR deficiency
- Published
- 2004
34. The [Na.sup.+]-[K.sup.+]-ATPase [[alpha].sub.2]-subunit isoform modulates contractility in the perinatal mouse diaphragm
- Author
-
Radzyukevich, Tatiana L., Moseley, Amy E., Shelly, Daniel A., Redden, Gregory A., Behbehani, Michael M., Lingrel, Jerry B., Paul, Richard J., and Heiny, Judith A.
- Subjects
Physiology -- Research ,Mice as laboratory animals -- Research ,Biological sciences - Abstract
The [Na.sup.+]-[K.sup.+]-ATPase [[alpha].sub.2]-subunit isoform modulates contractility in the perinatal mouse diaphragm. Am J Physiol Cell Physiol 287: C1300-C1310, 2004. First published July 14, 2004: doi:10.1152/ajpcell.00231.2004.--This study uses genetically altered mice to examine the contribution of the [Na.sup.+]-[K.sup.+]-ATPase [[alpha].sub.2] catalytic subunit to resting potential, excitability, and contractility of the perinatal diaphragm. The [[alpha].sub.2] protein is reduced by 38% in [[alpha].sub.2]-heterozygous and absent in [[alpha].sub.2]-knockout mice, and [[alpha].sub.1]-isoform is upregulated 1.9-fold in [[alpha].sub.2]-knockout. Resting potentials are depolarized by 0.8-4.0 mV in heterozygous and knockout mice. Action potential threshold, overshoot, and duration are normal. Spontaneous firing, a developmental function, is impaired in knockout diaphragm, but this does not compromise its ability to fire evoked action potential trains, the dominant mode of activation near birth. Maximum tetanic force, rate of activation, force-frequency and force-voltage relationships, and onset and magnitude of fatigue are not changed. The major phenotypic consequence of reduced [[alpha].sub.2] content is that relaxation from contraction is 1.7-fold faster. This finding reveals a distinct cellular role of the [[alpha].sub.2]-isoform at a step after membrane excitation, which cannot be restored simply by increasing [[alpha].sub.1] content. [Na.sup.+]/[Ca.sup.2+] exchanger expression decreases in parallel with [[alpha].sub.2]-isoform, suggesting that [Ca.sup.2+] extrusion is affected by the altered [[alpha].sub.2] genotype. There are no major compensatory changes in expression of sarcoplasmic reticulum [Ca.sup.2+]-ATPase, phospholamban, or plasma membrane [Ca.sup.2+]-ATPase. These results demonstrate that the [Na.sup.+]- [K.sup.+]-ATPase [[alpha].sub.1]-isoform alone is able to maintain equilibrium [K.sup.+] and [Na.sup.+] gradients and to substitute for [[alpha].sub.2]-isoform in most cellular functions related to excitability and force. They further indicate that the [[alpha].sub.2]-isoform contributes significantly less at rest than expected from its proportional content but can modulate contractility during muscle contraction. [Na.sup.+]-[K.sup.+]-ATPase [[alpha].sub.2] catalytic subunit; heterozygous mice; knockout mice; resting potential
- Published
- 2004
35. Myelin proteolipid protein-specific [CD4.sup.+][CD25.sup.+] regulatory cells mediate genetic resistance to experimental autoimmune encephalomyelitis
- Author
-
Reddy, Jayagopala, Illes, Zsolt, Zhang, Xingmin, Encinas, Jeffrey, Pyrdol, Jason, Nicholson, Lindsay, Sobel, Raymond A., Wucherpfennig, Kai W., and Kuchroo, Vijay K.
- Subjects
Myelin proteins -- Influence ,Myelin proteins -- Research ,Mice as laboratory animals -- Research ,Encephalomyelitis -- Research ,Encephalomyelitis -- Causes of ,Science and technology - Abstract
SJL mice are highly susceptible to experimental autoimmune encephalomyelitis (EAE) induced with myelin proteolipid protein (PLP) peptide 139-151, whereas H-2 congenic B10.S mice are resistant. Immunodominance and susceptibility to EAE are associated with a high precursor frequency of PLP 139-151-specific T cells in the naive repertoire of SJL mice. To understand the mechanism of EAE resistance in B10.S mice, we determined the precursor frequency of PLP 139-151-reactive T cells in both strains by using I[A.sup.s]/PLP 139-151 tetramers. SJL and B10.S mice had similar frequencies of tetramer-reactive T cells in the naive peripheral repertoire. However, in SJL mice, the majority of PLP 139-151 tetramer-positive cells were in the [CD4.sup.+][CD25.sup.-] population, whereas there were more tetramer-positive cells in the [CD4.sup.+][CD25.sup.+] population of B10.S mice. Depletion of [CD4.sup.+][CD25.sup.+] cells in vivo facilitated the expansion of pip 139-151-reactive cells with production of T helper 1 cytokines in EAE-resistant B10.S mice. Furthermore, anti-CD25 Ab treatment before immunization resulted in EAE induction in these otherwise resistant mice. These data indicate an important role for autoantigen-specific [CD4.sup.+][CD25.sup.+] cells in genetic resistance to autoimmunity.
- Published
- 2004
36. Mutation rate and predicted phenotypic target sizes in ethylnitrosourea-treated mice
- Author
-
Concepcion, Dorothy, Seburn, Kevin L., Wen, Gen, Frankel, Wayne N., and Hamilton, Bruce A.
- Subjects
Mice as laboratory animals -- Research ,Genetic research ,Gene mutations ,Biological sciences - Abstract
Chemical mutagenesis of the mouse is ongoing in several centers around the world, with varying estimates of mutation rate and number of sites mutable to phenotype. To address these questions, we sequenced ~9.6 Mb of DNA from [G.sub.1] progeny of ethylnitrosourea-treated mice in a large, broad-spectrum screen. We identified 10 mutations at eight unique sites, including six nonsynonymous coding substitutions. This calibrates the nucleotide mutation rate for two mutagenesis centers, implies significance criteria for positional cloning efforts, and provides working estimates of effective genetic target sizes for selected phenotypes.
- Published
- 2004
37. The effects of aging and treadmill running on soleus and gastrocnemius muscle morphology in the senescence-accelerated mouse (SAMP1)
- Author
-
Sakakima, Harutoshi, Yoshida, Yoshihiro, Suzuki, Shusaku, and Morimoto, Norio
- Subjects
Mice as laboratory animals -- Research ,Exercise -- Influence ,Aging -- Influence ,Extremities, Lower -- Muscles ,Extremities, Lower -- Physiological aspects ,Leg -- Muscles ,Leg -- Physiological aspects ,Health ,Seniors - Abstract
We investigated the effects of aging on the soleus and gastrocnemius muscles in male SAMP1 (senescence-accelerated mouse prone 1). Body mass, muscle wet weight, fiber size, and the percent of type II fibers declined from 50 weeks of age. Voluntary motor behavior also significantly declined with age. Furthermore, we examined the effects of high (twice daily) and low (once daily) frequency treadmill running, for 6 weeks at 5 days per week, beginning when the mice were 50 weeks old. Muscle fiber size for the high frequency running significantly increased. Pathological fiber alterations in these mice were increased by running, especially by high frequency running. This suggests that age-related muscle morphological changes in SAMP1 occurs from 50 weeks of age, and that the decline in voluntary motor behavior is an important factor in aging muscle atrophy. In addition, high frequency running is more beneficial for aged muscle hypertrophy. This model is useful for studying the acceleration of the aging process in skeletal muscle of the SAM.
- Published
- 2004
38. Multiple roles for Nodal in the epiblast of the mouse embryo in the establishment of anterior-posterior patterning
- Author
-
Lu, Cindy C. and Robertson, Elizabeth J.
- Subjects
Mice as laboratory animals -- Research ,Cell research ,Biological sciences - Abstract
The TGF[beta] family member Nodal has been shown to be involved in a variety of processes in development, including early axis formation. Here, we use a conditional gene inactivation strategy to show a specific requirement for Nodal in the epiblast. Complete inactivation of the Nodal locus in the epiblast using the Sox2-Cre deleter strain results in a failure to establish global anterior-posterior patterning, a phenotype that resembles the Nodal null phenotype. By contrast, mosaic inactivation of Nodal in the epiblast using the Mox2-Cre (MORE) deleter strain affects formation of the anterior mesendoderm and subsequent anterior neureetoderm patterning. Furthermore, ES cell chimera experiments indicate that Nodal-deficient ES cells preferentially populate the anterior compartment of the epiblast, suggesting that cell mixing in the epiblast is not random and that Nodal signaling mediates a novel anterior-posterior cell-sorting process within the epiblast before gastrulation. Keywords: Nodal; Mouse; Embryo; Epiblast; Anterior-posterior; Gastrulation; Cell mixing; Cell sorting; AVE; Anterior mesendoderm
- Published
- 2004
39. Disruption of growth hormone receptor gene causes diminished pancreatic islet size and increased insulin sensitivity in mice
- Author
-
Liu, Jun-Li, Coschigano, Karen T., Robertson, Katie, Lipsett, Mark, Guo, Yubin, Kopchick, John J., Kumar, Ujendra, and Liu, Ye Lauren
- Subjects
Mice as laboratory animals -- Research ,Glucose tolerance tests ,Growth ,Somatotropin ,Biological sciences - Abstract
Growth hormone, acting through its receptor (GHR), plays an important role in carbohydrate metabolism and in promoting postnatal growth. GHR gene-deficient (GH[R.sup.-/-]) mice exhibit severe growth retardation and proportionate dwarfism. To assess the physiological relevance of growth hormone actions, GH[R.sup.-/-] mice were used to investigate their phenotype in glucose metabolism and pancreatic islet function. Adult GH[R.sup.-/-] mice exhibited significant reductions in the levels of blood glucose and insulin, as well as insulin mRNA accumulation. Immunohistochemical analysis of pancreatic sections revealed normal distribution of the islets despite a significantly smaller size. The average size of the islets found in GH[R.sup.-/-] mice was only one-third of that in wild-type littermates. Total [beta]-cell mass was reduced 4.5-fold in GH[R.sup.-/-] mice, significantly more than their body size reduction. This reduction in pancreatic islet mass appears to be related to decreases in proliferation and cell growth. GH[R.sup.-/-] mice were different from the human Laron syndrome in serum insulin level, insulin responsiveness, and obesity. We conclude that growth hormone signaling is essential for maintaining pancreatic islet size, stimulating islet hormone production, and maintaining normal insulin sensitivity and glucose homeostasis. glucose homeostasis; immunohistochemistry; Laron syndrome; insulin tolerance test
- Published
- 2004
40. Effect of Th1 Cytokines on Acid Secretion in Pharmacologically Characterised Mouse Gastric Glands
- Author
-
Padol, I. T. and Hunt, R. H.
- Subjects
Pylorus -- Causes of ,Gastritis -- Causes of ,Helicobacter pylori -- Complications and side effects ,Mice as laboratory animals -- Research ,Health - Published
- 2004
41. Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis
- Author
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Stevens, Mark P., Haque, Ashraful, Atkins, Timothy, Hill, Jim, Wood, Michael W., Easton, Anna, Nelson, Michelle, Underwood-Fowler, Cindy, Titball, Richard W., Bancroft, Gregory J., and Galyov, Edouard E.
- Subjects
Mice as laboratory animals -- Research ,Pseudomonas infections -- Care and treatment ,Pseudomonas -- Research ,Biological sciences - Abstract
Melioidosis is a severe infectious disease of animals and humans caused by the Gram-negative intracellular pathogen Burkholderia pseudomallei. An Inv/Mxi-Spa-like type III protein secretion apparatus, encoded by the B. pseudomallei bsa locus, facilitates bacterial invasion of epithelial cells, escape from endocytic vesicles and intracellular survival. This study investigated the role of the Bsa type III secretion system in the pathogenesis of melioidosis in murine models. B. pseudomallei bipD mutants, lacking a component of the translocation apparatus, were found to be significantly attenuated following intraperitoneal or intranasal challenge of BALB/c mice. Furthermore, a bipD mutant was attenuated in C57BL/6 IL-12 p40-/- mice, which are highly susceptible to B. pseudomallei infection. Mutation of bipD impaired bacterial replication in the liver and spleen of BALB/c mice in the early stages of infection. B. pseudomallei mutants lacking either the type III secreted guanine nucleotide exchange factor BopE or the putative effectors BopA or BopB exhibited varying degrees of attenuation, with mutations in bopA and bopB causing a significant delay in median time to death. This indicates that bsa-encoded type III secreted proteins may act in concert to determine the outcome of B. pseudomallei infection in mice. Mice inoculated with the B. pseudomallei bipD mutant were partially protected against subsequent challenge with wild-type B. pseudomallei. However, immunization of mice with purified BipD protein was not protective.
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- 2004
42. Phenotypic variation resulting from a deficiency of epidermal growth factor receptor in mice is caused by extensive genetic heterogeneity that can be genetically and molecularly partitioned
- Author
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Strunk, Karen E., Amann, Vicky, and Threadgill, David W.
- Subjects
Mice as laboratory animals -- Research ,Genetic research ,Epidermal growth factor ,Biological sciences - Abstract
The timing of lethality caused by homozygosity for a null allele of the epidermal growth factor receptor [Egfr.sup.tm1Mag] in mice is strongly dependent on genetic background. Initial attempts to genetically map background modifiers using Swiss-derived, outbred CD-1 mice were unsuccessful. To investigate the genetic architecture contributing to survival of [Egfr.sup.tm1Mag] homozygous embryos, the genetic variability segregating within the outbred population was partitioned by surveying viability of [Egfr.sup.tm1Mag] mutants using intercrosses between 129S6/SvEvTAC-[Egfr.sup.tm1Mag] and nine Swiss-derived, inbred strains: ALR/LtJ, ALS/LtJ, APN, APS, ICR/HaRos, NOD/LtJ, NON/LtJ, SJL/J, and SWR/J. The observations showed that these strains support varying levels of survival of [Egfr.sup.tm1Mag] homozygous embryos, suggesting that genetic heterogeneity within the CD-1 stock contributed to the original lack of [Egfr.sup.tm1Mag] modifier detection. Simlar to the Swiss-derived intercrosses, nine congenic strains, derived from 129S6/SvEvTAC, AKR/J, APN, BALB/cJ, BTBR-[T.sup.+] tf/tf C3H/HeJ, C57BL/6J, DBA/2J, and FVB/NJ inbred backgrounds, also supported varying levels of survival of [Egfr.sup.tm1Mag] mutants. By intercrossing the congenic lines to create hybrid [F.sub.1] embryos, different genetic backgrounds were found to have complementary modifiers. Analysis of the congenic lines argues against heterosis of outbred backgrounds contributing to [Egfr.sup.tm1Mag] phenotypic variability. A detailed analysis of the crosses suggests that modifiers function at three distinct stages of development. One class of modifiers supports survival of [Egfr.sup.tm1Mag] homozygous embryos to mid-gestation, another class supports development through the mid-gestation transition from yolk-sac to placental-derived nutrient sources, and a third class supports survival through later stages of gestation. Data from microarray analysis using RNA from wild-type and [Egfr.sup.tm1Mag] mutant placentas support the existence of extensive genetic heterogeneity and suggest that it can be molecularly partitioned. This method should be generally useful to partition heterogeneity contributing to other complex traits.
- Published
- 2004
43. In vivo interaction between mitochondria carrying mtDNAs from different mouse species
- Author
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Sato, Akitsugu, Nakada, Kazuto, Shitara, Hiroshi, Yonekawa, Hiromichi, and Hayashi, Jun-Ichi
- Subjects
Mice as laboratory animals -- Research ,Mitochondrial DNA ,Biological sciences - Abstract
Mitochondrial disease model mice, mitomice, were created using zygotes of B6mtspr strain mice carrying mitochondrial DNA (mtDNA) from Mus spretus as recipients of exogenous mitochondria carrying wild-type and a deletion mutant mtDNA ([DELTA]mtDNA) of M. musculus domesticus. In these experiments, mtDNAs from different mouse species were used for identification of exo- and endogenous wild-type mtDNAs in the mitomice. Results showed transmission of exogenous [DELTA]mtDNA, but not exogenous wild-type mtDNA, of M. m. domesticus to following generations through the female germ line. Complete elimination of exogenous wild-type mtDNA would be due to stochastic segregation, whereas transmission of exogenous [DELTA]mtDNA would be due to its smaller size leading to a propagational advantage. Tissues in mitomice of the [F.sub.3] generation carrying exogenous [DELTA]mtDNA showed protection from respiration defects until AmtDNA accumulated predominantly. This protection from expression of mitochondrial dysfunction was attained with the help of endogenous wild-type mtDNA of M. spretus, since mitomice did not possess exogenous wild-type mtDNA of M. m. domesticus. These observations provide unambiguous evidence for the presence of interaction between exogenous mitochondria carrying [DELTA]mtDNA and endogenous mitochondria carrying M. spretus wild-type mtDNA.
- Published
- 2004
44. Effects of obesity on the relationship of leptin mRNA expression and adipocyte size in anatomically distinct fat depots in mice
- Author
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Guo, Kai-Ying, Halo, Patricia, Leibel, Rudolph L., and Zhang, Yiying
- Subjects
Tumor necrosis factor -- Research ,Leptin -- Research ,Mice as laboratory animals -- Research ,Mice as laboratory animals -- Physiological aspects ,Biological sciences - Abstract
In support of leptin's physiological role as humoral signal of fat mass, we have shown that adipocyte volume is a predominant determinant of leptin mRNA levels in anatomically distinct fat depots in lean young mice in the postabsorptive state. In this report, we investigated how obesity may affect the relationship between leptin mRNA levels and adipocyte volume in anatomically distinct fat depots in mice with genetic ([Lep.sub.ob]/[Lep.sub.ob] and [A.sup.y/+), diet-induced, and aging-related obesity. In all of the obese mice examined, tissue leptin mRNA levels relative to the average adipocyte volume were lower in the perigonadal and/or retroperitoneal than in the inguinal fat depots and were lower than those of the lean young mice in the perigonadal fat depot. A close, positive correlation between leptin mRNA level and adipocyte volume was present from small to hypertrophic adipocytes within each perigonadal and inguinal fat pad in the obese mice, but the slopes of the regression lines relating leptin mRNA level to adipocyte volume were significantly lower in the perigonadal than in the inguinal fat pads of the same mice. These results suggest that obesity per se is associated with a decreased leptin gene expression per unit of fat mass in mice and that the positive correlation between leptin mRNA level and adipocyte volume is an intrinsic property of adipocytes that is not disrupted by adipocyte hypertrophy in obese mice. size fractionation; glucocorticoid receptor; tumor necrosis factor-[alpha]
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- 2004
45. Sleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes
- Author
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Shiromani, Priyattam J., Xu, Man, Winston, Elizabeth M., Shiromani, Samara N., Gerashchenko, Dmitry, and Weaver, David R.
- Subjects
Genetic regulation -- Research ,Mice as laboratory animals -- Research ,Mice as laboratory animals -- Physiological aspects ,Sleep-wake cycle -- Research ,Biological sciences - Abstract
In mammals, sleep is regulated by circadian and homeostatic mechanisms. The circadian component, residing in the suprachiasmatic nucleus (SCN), regulates the timing of sleep, whereas homeostatic factors determine the amount of sleep. It is believed that these two processes regulating sleep are independent because sleep amount is unchanged after SCN lesions. However, because such lesions necessarily damage neuronal connectivity, it is preferable to investigate this question in a genetic model that overcomes the confounding influence of circadian rhythmicity. Mice with disruption of both mouse Period genes (mPer)l and mPer2 have a robust diurnal sleep-wake rhythm in an entrained light-dark cycle but lose rhythmicity in a free-run condition. Here, we examine the role of the roper genes on the rhythmic and homeostatic regulation of sleep. In entrained conditions, when averaged over the 24-h period, there were no significant differences in waking, slow-wave sleep (SWS), or rapid eye movement (REM) sleep between mPer1, mPer2, mPer3, mPer1-mPer2 double-mutant, and wild-type mice. The mice were then kept awake for 6 h (light period 6-12), and the mPer mutants exhibited increased sleep drive, indicating an intact sleep homeostatic response in the absence of the mPer genes. In free-run conditions (constant darkness), the mPer1-mPer2 double mutants became arrhythmic, but they continued to maintain their sleep levels even after 36 days in free-running conditions. Although mPer1 and mPer2 represent key elements of the molecular clock in the SCN, they are not required for homeostatic regulation of the daily amounts of waking, SWS, or REM sleep. circadian rhythmicity; rapid eye movement sleep
- Published
- 2004
46. Recent advances in alcoholic liver disease: II. Minireview: molecular mechanisms of alcoholic fatty liver
- Author
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You, Min and Crabb, David W.
- Subjects
Mice as laboratory animals -- Research ,Alcohol, Denatured -- Influence ,Alcohol, Denatured -- Adverse and side effects ,Alcohol -- Influence ,Alcohol -- Adverse and side effects ,Biological sciences - Abstract
Alcohol has long been thought to cause fatty liver by way of altered NADH/NA[D.sup.+] redox potential in the liver, which, in turn, inhibits fatty acid oxidation and the activity of tricarboxylic acid cycle reactions. More recent studies indicate that additional effects of ethanol both impair fat oxidation and stimulate lipogenesis. Ethanol interferes with DNA binding and transcription-activating properties of peroxisome proliferator-activated receptor-[alpha] (PPAR[alpha]), as demonstrated with cultured cells and in ethanol-fed mice. Treatment of ethanol-fed mice with a PPAR[alpha] agonist can reverse fatty liver even in the face of continued ethanol consumption. Ethanol also activated sterol regulatory element binding protein 1, inducing a battery of lipogenic enzymes. These effects may be due in pan to inhibition of AMP-dependent protein kinase, reduction in plasma adiponectin, or increased levels of TNF-[alpha] in the liver. The understanding of these ethanol effects provides new therapeutic targets to reverse alcoholic fatty liver. triglyceride; lipid; transcription factor; ethanol
- Published
- 2004
47. Differential regulation of fatty acid trapping in mouse adipose tissue and muscle by ASP
- Author
-
Faraj, May and Cianflone, Katherine
- Subjects
Proteins -- Influence ,Mice as laboratory animals -- Research ,Acylation ,Biological sciences - Abstract
Acylation-stimulating protein (ASP) is a lipogenic hormone secreted by white adipose tissue (WAT). Male C3 knockout (KO; [C3.sup.-/-]) ASP-deficient mice have delayed postprandial triglyceride (TG) clearance and reduced WAT mass. The objective of this study was to examine the mechanism(s) by which ASP deficiency induces differences in postprandial TG clearance and body composition in male KO mice. Except for increased [sup.3]H-labeled nonesterified fatty acid (NEFA) trapping in brown adipose tissue (BAT) of KO mice (P = 0.02), there were no intrinsic tissue differences between wild-type (WT) and KO mice in [sup.3]H-NEFA or [[sup.14]C]glucose oxidation, TG synthesis or lipolysis in WAT, muscle, or liver. There were no differences in WAT or skeletal muscle hydrolysis, uptake, and storage of [[sup.3]H]triolein substrate [in situ lipoprotein lipase (LPL) activity]. ASP, however, increased in situ LPL activity in WAT (+64.8%, P = 0.02) but decreased it in muscle (-35.0%, P = 0.0002). In addition, after prelabeling WAT with [[sup.3]H]oleate and [[sup.14]C]glucose, ASP increased [sup.3]H-lipid retention, [[sup.3]H]TG synthesis, and [[sup.3]H]TG-to-[[sup.14]C]TG ratio, whereas it decreased [sup.3]H-NEFA release, indicating increased NEFA trapping in WAT. Conversely, in muscle, ASP induced effects opposite to those in WAT and increased lipolysis, indicating reduced NEFA trapping within muscle by ASP (P < 0.05 for all parameters). In conclusion, novel data in this study suggest that 1) there is little intrinsic difference between KO and WT tissue in the parameters examined and 2) ASP differentially regulates in situ LPL activity and NEFA trapping in WAT and skeletal muscle, which may promote optimal insulin sensitivity in vivo. acylation-stimulating protein; C3adesArg; fatty acid; triglyceride; lipoprotein lipase; brown adipose tissue
- Published
- 2004
48. Sim1 gene dosage modulates the homeostatic feeding response to increased dietary fat in mice
- Author
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Holder, J. Lloyd, Jr., Zhang, Ling, Kublaoui, Bassil M., DiLeone, Ralph J., Oz, Orhan K., Bair, Chi Horng, Lee, Ying-Hue, and Zinn, Andrew R.
- Subjects
Mice as laboratory animals -- Research ,Hyperphagia -- Influence ,Diet -- Influence ,Biological sciences - Abstract
Haploinsufficiency of the transcription factor gene Sim1 has been previously implicated in hyperphagic obesity in humans and mice. To investigate the relation between Sim1 dosage and hyperphagia, we generated sim1-knockout mice and studied their growth and feeding behavior. Heterozygous mice weaned on standard chow consumed 14% more food per day than controls and developed obesity, hyperinsulinemia, and hyperleptinemia. The sim1 heterozygous mice were also significantly longer than controls. Heterozygous animals had modestly increased feeding efficiency, suggesting reduced energy expenditure, but voluntary wheel-running activity did not differ significantly between the two groups. We studied the effect of dietary fat on the feeding behavior of heterozygous sim1 mutant mice. The tempo and severity of weight gain were much greater in animals weaned on a high-fat diet. When acutely challenged with increased dietary fat, sim1 heterozygous mice weaned on the chow diet markedly increased their food consumption and caloric intake, whereas control mice reduced the mass of food they consumed and maintained approximately isocaloric intake. In wild-type adult mice, we detected Sim1 expression in the paraventricular and supraoptic nuclei, as previously reported in neonates, as well as in the amygdala and lateral hypothalamus, all regions implicated in feeding behavior. Our results indicate that Sim1 gene dosage modulates the homeostatic feeding response to increased dietary fat and likely plays a physiological role in the regulation of energy balance. hypothalamus; transcription factor; feeding behavior
- Published
- 2004
49. Long-term combined beneficial effects of physical training and metabolic treatment on atherosclerosis in hypercholesterolemic mice
- Author
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Napoli, Claudio, Williams-Ignarro, Sharon, de Nigris, Filomena, Lerman, Lilach O., Rossi, Loredana, Guarino, Carmen, Mansueto, Gelsomina, Di Tuoro, Francesco, Pignalosa, Orlando, De Rosa, Gaetano, Sica, Vincenzo, and Ignarro, Louis J.
- Subjects
Lipoprotein A -- Research ,Atherosclerosis -- Research ,Atherosclerosis -- Care and treatment ,Mice as laboratory animals -- Research ,Science and technology - Abstract
The pathogenic mechanisms by which physical exercise influences atherosclerotic lesion formation remain poorly understood. Because vigorous physical training increases oxidative stress, this study tested the hypothesis that graduated and moderate physical exercise together with metabolic intervention (L-arginine and antioxidants) may contribute to increased vascular protection. Exercise training in mite was induced by graduated swimming. In hypercholesterolemic male mice on an atherogenic high-cholesterol diet, graduated and moderate exercise lowered plasma cholesterol and decreased atherosclerotic lesions compared with sedentary control mice. Antioxidants (1.0% vitamin E added to the chow and 0.05% vitamin C added to the drinking water) and L-arginine (6% in drinking water) supplementation to exercising hypercholesterolemic mite further and synergistically reduced atherosclerosis compared with untreated exercised mice. Arterial oxidation-specific epitopes and systemic oxidative stress were reduced by metabolic intervention. Graduated chronic exercise elicited an increase in production of nitric oxide through increased endothelial nitric oxide synthase expression and ameliorated scavenger activities. Thus, metabolic intervention with L-arginine and antioxidants together with graduated and moderate exercise training reduce atherosclerotic lesion formation. catalase | nitric oxide synthase | vitamin E | oxidative stress | low-density lipoprotein
- Published
- 2004
50. Lethal impairment of cholinergic neurotransmission in hemicholinium-3-sensitive choline transporter knockout mice
- Author
-
Ferguson, Shawn M., Bazalakova, Mihaela, Savchenko, Valentina, Tapia, Juan Carlos, Wright, Jane, and Blakely, Randy D.
- Subjects
Cholinergic mechanisms -- Research ,Mice as laboratory animals -- Research ,Neuromuscular blocking agents -- Research ,Acetylcholine -- Research ,Science and technology - Abstract
Presynaptic acetylcholine (ACh) synthesis and release is thought to be sustained by a hemicholinium-3-sensitive choline transporter (CHT). We disrupted the murine CHT gene and examined CHT-/and +/- animals for evidence of impaired cholinergic neurotransmission. Although morphologically normal at birth, CHT-/- mice become immobile, breathe irregularly, appear cyanotic, and die within an hour. Hemicholinium-3-sensitive choline uptake and subsequent ACh synthesis are specifically lost in CHT-/- mouse brains. Moreover, we observe a time-dependent loss of spontaneous and evoked responses at CHT-/- neuromuscular junctions. Consistent with deficits in synaptic ACh availability, we also observe developmental alterations in neuromuscular junction morphology reminiscent of changes in mutants lacking ACh synthesis. Adult CHT+/- mice overcome reductions in CHT protein levels and sustain choline uptake activity at wild-type levels through posttranslational mechanisms. Our results demonstrate that CHT is an essential and regulated presynaptic component of cholinergic signaling and indicate that CHT warrants consideration as a candidate gene for disorders characterized by cholinergic hypofunction.
- Published
- 2004
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