Search

Secondary structure of tRNA His and observed hisR mutations. This file (.pdf) contains a figure showing the secondary structure of tRNA His and observed hisR mutations aligned with tRNA numbering convention. (PDF 272 kb)

Genome duplication amplification events observed in cysK, carA, and ptsI experiments. This file (.pdf) contains a figure showing the read depth coverage (y-axis) plotted against the genome position (x-axis) for flask 1 population samples for A. cysK, B. carA, C. and ptsI experiments. (PDF 316 kb)

FBA simulations for genes listed in Table 1. This file (.pdf) contains a table of simulations demonstrating the same predicted behavior in both the iJO1366 model corresponding to the MG1655 genotype and a modified iJO1366 model corresponding to the BW25113 genotype.(PDF 79 kb)

Growth curves of five false positive replicate strains (ptsI, serB, proA, proB, and carA) in separate subplots are displayed. This file (.pdf) contains a figure that is an extension of Figure 1B, as the same curves for these cases are displayed; however, specific labeling of each curve with their associated experiment (exp.) number is provided in the legend of each subplot. The experiment number corresponds to those numbers listed in Table 2. (PDF 183 kb)

Contingency table examples showing the likelihood of mutational convergence. This file (.pdf) contains a figure of contingency table examples showing the likelihood of mutational convergence between two samples. p-values calculated from Fisherâ s exact test are reported for four examples. (PDF 97 kb)

All FP KO strains considered in this study. This file (.pdf) contains a table listing all false positive KO strains considered for extended growth tests. Additional information regarding proposed conditional essentiality is also provided as well as a comparison to a previous study [22]. (PDF 67 kb)

Mutations observed in starting strains grown in LB rich medium. This file (.pdf) contains a table listing mutations identified in the PCR-confirmed Keio strains that were used in the extended growth tests on minimal medium. (PDF 49 kb)