Search

Abstract Despite the end of the global Coronavirus Disease 2019 (COVID-19) pandemic, the risk factors for COVID-19 severity continue to be a pivotal area of research. Specifically, studying the impact of the genomic diversity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) on COVID-19 severity is crucial for predicting severe outcomes. Therefore, this study aimed to investigate the impact of the SARS-CoV-2 genome sequence, genotype, patient age, gender, and vaccination status on the severity of COVID-19, and to develop accurate and robust prediction models. The training set (n = 12,038), primary testing set (n = 4,006), and secondary testing set (n = 2,845) consist of SARS-CoV-2 genome sequences with patient information, which were obtained from Global Initiative on Sharing all Individual Data (GISAID) spanning over four years. Four machine learning methods were employed to construct prediction models. By extracting SARS-CoV-2 genomic features, optimizing model parameters, and integrating models, this study improved the prediction accuracy. Furthermore, Shapley Additive exPlanes (SHAP) was applied to analyze the interpretability of the model and to identify risk factors, providing insights for the management of severe cases. The proposed ensemble model achieved an F-score of 88.842% and an Area Under the Curve (AUC) of 0.956 on the global testing dataset. In addition to factors such as patient age, gender, and vaccination status, over 40 amino acid site mutation characteristics were identified to have a significant impact on the severity of COVID-19. This work has the potential to facilitate the early identification of COVID-19 patients with high risks of severe illness, thus effectively reducing the rates of severe cases and mortality.

Abstract Letermovir (LTV) prophylaxis is effective in reducing the incidence of clinically significant cytomegalovirus (CMV) infection (cs CMVi) after allogeneic haematopoietic stem cell transplantation (allo-HSCT). Since our centre began administering LTV prophylaxis in June 2022, we have observed a certain increase in the incidence of Epstein–Barr virus (EBV) reactivation after haploidentical HSCT. We retrospectively analysed 230 consecutive patients who underwent haploidentical HSCT with rabbit anti-thymocyte globulin (ATG) from October 2022 to June 2023. The LTV group included 133 patients who received LTV prophylaxis, and the control group included 97 patients who did not receive LTV prophylaxis. At 1 year after HSCT, EBV reactivation was observed in 36 patients (27%) in the LTV group and 13 patients (13%) in the control group (p = 0.012). All patients with EBV reactivation had EBV-DNAemia, and one patient in each group developed EBV-associated posttransplantation lymphoproliferative disorder (PTLD). The proportion of patients with low EBV-DNA loads (> 5 × 102 to

Abstract Rural preschool education is an integral part of rural society, and improving the efficiency system of evaluation of rural preschool education resource allocation is an important strategy for the implementation of the rural revitalization. This paper uses an input-oriented three-stage DEA model to analyze the efficiency of rural preschool education resource allocation in 30 provinces in China from 2012 to 2020. The results show that external factors such as the level of urbanization, birth rate, and the scale of kindergarten impacts the efficiency of rural preschool education resource allocation significantly. Without regard to the influence of environmental and random factors, the overall trend of the average efficiency of rural preschool education resource allocation in China has improved, showing a regional pattern of “central > eastern > western.” Therefore, based on the relevant policies, this paper puts forward rational suggestions for the improvement of rural preschool resource allocation efficiency in China from the perspectives of human, financial and material resources.

Abstract Blinatumomab has emerged as a promising component of first-line therapy for acute B-cell precursor lymphoblastic leukemia (BCP-ALL), bolstering treatment efficacy. To mitigate CD19 selection pressure and reduce the incidence of blinatumomab-associated toxicities, pre-treatment chemotherapy is recommended before administering blinatumomab. From September 2022 to December 2023, we conducted a single-arm, multicenter, phase 2 trial (NCT05557110) in newly diagnosed Philadelphia chromosome-negative BCP-ALL (Ph-negative BCP-ALL) patients. Participants received induction treatment with reduced-dose chemotherapy (RDC), comprising idarubicin, vindesine, and dexamethasone over 7 days, followed by 2 weeks of blinatumomab. Those failing to achieve composite complete remission (CRc) received an additional 2 weeks of blinatumomab. The primary endpoint was the CRc rate post initial induction treatment. Of the 35 enrolled patients, 33 (94%) achieved CRc after 2 weeks of blinatumomab, with 30 (86%) achieving measurable residual disease (MRD) negativity. Two patients extended blinatumomab to 4 weeks. With either 2 or 4 weeks of blinatumomab treatment, all patients achieved CR (35/35) and 89% (31/35) were MRD negativity. The median time to CR was 22 days. Immune effector cell-associated neurotoxicity syndrome was limited (14%, all grade 1). Non-hematological adverse events of grade 3 or higher included pneumonia (17%), sepsis (6%), and cytokine release syndrome (9%). With a median follow-up of 11.5 months, estimated 1-year overall survival and 1-year progression-free survival rates were 97.1% and 82.2%, respectively. These findings affirm that RDC followed by blinatumomab is an effective and well-tolerated induction regimen for newly diagnosed Ph-negative BCP-ALL, supporting a shift towards less intensive and more targeted therapeutic approaches. Trial registration: https://www.clinicaltrials.Gov . Identifier NCT05557110.

ABSTRACT: Yogurt usually contains 5% to 7% sugar and 3% to 5% lactose. As β-galactosidases can hydrolyze lactose and improve sweetness, they have the potential to produce lactose-free (LF) and no-sugar-added (NSA) yogurt. In this study, the β-galactosidase AoBgal35A from Aspergillus oryzae was engineered by site-saturation mutagenesis. Results of 19 variants of T955 residue showed that the lactose hydrolysis rate of T955R-AoBgal35A was up to 90.7%, which is much higher than the 78.5% of the wild type. Moreover, the optimal pH of T955R-AoBgal35A was shifted from pH 4.5 to pH 5.5, and the optimal temperature decreased from 60°C to 50°C. The mutant T955R-AoBgal35A was successfully expressed in Komagataella pastoris, which produced extracellularly 4,528 U/mL of β-galactosidase activity. The mutant T955R-AoBgal35A was used to produce LF yogurt. The Streptococcus thermophilus count of LF yogurt increased from 7.9 to 9.5 log cfu/g, which is significantly higher than that of the control group (8.9 log cfu/g). The residual lactose content of LF yogurt was 0.13%, meeting the requirements of the national standard in China for the “lactose-free” label (

This is an article in the field of mining engineering. Li-Be-Nb-Ta are not only the important highly-demanded rare metals, but also key minerals in emerging strategic industries. They have both commonality and individuality in mineralization mechanism and R&D with new progress. This article aims to comprehensively and accurately measure and evaluate the global resource and reserve data of Li-Be-Nb-Ta, and timely reflect new developments in exploration and development and technological progress. The multi-cycle metallogenic theory of granite pegmatite has made important breakthrough in exploration, the technical output of lithium deposits in South American salt lakes is approaching, the potential for lithium extraction and storage increase in clay rocks and geothermal brines is increasing, the alkaline-peralkaline volcanic intrusive pegmatite type Nb-Ta mineralization has been highlighted in the Americas and the Qinghai-Xizang region, and the exploration of Li-Be deposits in mainland China has made important breakthroughs. The construction of the resource base for Nb-Ta-REE in northwest Hubei can learn from the progress and experience of enterprise level exploration and testing such as Avalon Rare Metals Company in North America. Based on the analysis of 1396 lithium beryllium niobium tantalum deposits and typical cases in the world, this article proposes that multi stage thermal events and multi cycle magmatic differentiation and melting may be the key conditions for large to super large deposits.

Abstract. Objectives. This study aims to not only investigate the prevalence of social alienation among elderly patients undergoing radical prostatectomy for prostate cancer but also identify the contributing factors. Materials and methods. A total of 245 elderly patients diagnosed with prostate cancer and undergoing radical prostatectomy at a tertiary care general hospital in Jinan were included in this study. To assess the patients, several questionnaires were used. These included the General Situation Questionnaire, General Alienation Scale, Social Impact Scale, Modified Memorial Anxiety Scale for Prostate Cancer, and Perceived Social Support Scale. Pearson correlation analysis was conducted to examine the relationships between variables, whereas multiple linear regression was used to identify the factors influencing social alienation among patients who underwent radical prostatectomy. Results. Patients who underwent radical prostatectomy had a mean total score of 44.13 ± 7.24 on the Social Alienation Scale. The results of the Pearson correlation analysis indicated that social alienation showed an inverse association with social support (r = −0.627, p < 0.05) and positive associations with age, disease stigma, and anxiety (r = 0.325, 0.575, 0.421, all p’s < 0.01) among patients who underwent radical prostatectomy. The findings from multiple linear regression analysis demonstrated that educational level, age, urinary incontinence, disease stigma, anxiety, and social support significantly influenced social alienation among elderly patients who underwent radical prostatectomy (p < 0.05). Conclusions. Elderly patients who undergo radical prostatectomy often experience social alienation. This study found that social alienation was associated with factors such as educational level, age, urinary incontinence, social support, anxiety, and disease stigma. Consequently, healthcare providers should actively monitor the degree of social alienation in elderly patients after radical prostatectomy and provide suitable psychological care to facilitate positive social reintegration and alleviate their feelings of social alienation.

Abstract Severe cytokine release syndrome (sCRS) and immune effector cell‐associated neurotoxicity syndrome (ICANS) have limited the widespread use of chimeric antigen receptor T (CAR T)‐cell therapy. We designed a novel anti‐CD19 CAR (ssCART‐19) with a small hairpin RNA (shRNA) element to silence the interleukin‐6 (IL‐6) gene, hypothesizing it could reduce sCRS and ICANS by alleviating monocyte activation and proinflammatory cytokine release. In a post hoc analysis of two clinical trials, we compared ssCART‐19 with common CAR T‐cells (cCART‐19) in relapsed/refractory B‐cell acute lymphoblastic leukemia (r/r B‐ALL). Among 87 patients, 47 received ssCART‐19 and 40 received cCART‐19. Grade ≥3 CRS occurred in 14.89% (7/47) of the ssCART‐19 group versus 37.5% (15/40) in the cCART‐19 group (p = 0.036). ICANS occurred in 4.26% (2/47) of the ssCART‐19 group (all grade 1) compared to 15% (2/40) of the cCART‐19 group. Patients in the ssCART‐19 group showed comparable rates of treatment response (calculated with rates of complete remission and incomplete hematological recovery) were 91.49% (43/47) for ssCART‐19 and 85% (34/40) for cCART‐19 (p = 0.999). With a median follow‐up of 21.9 months, cumulative nonrelapse mortality was 10.4% for ssCART‐19 and 13.6% for cCART‐19 (p = 0.33). Median overall survival was 37.17 months for ssCART‐19 and 32.93 months for cCART‐19 (p = 0.40). Median progression‐free survival was 24.17 months for ssCART‐19 and 9.33 months for cCART‐19 (p = 0.23). These data support the safety and efficacy of ssCART‐19 for r/r B‐ALL, suggesting its potential as a promising therapy.

PurposeThe overall survival of patients with pancreatic cancer is extremely low. We aimed to establish machine learning (ML) based model to accurately predict three-year survival and prognosis of pancreatic cancer patients.MethodsWe analyzed pancreatic cancer patients from the Surveillance, Epidemiology, and End Results (SEER) database between 2000 and 2021. Univariate and multivariate logistic analysis were employed to select variables. Recursive Feature Elimination (RFE) method based on 6 ML algorithms was utilized in feature selection. To construct predictive model, 13 ML algorithms were evaluated by area under the curve (AUC), area under precision-recall curve (PRAUC), accuracy, sensitivity, specificity, precision, cross-entropy, Brier scores and Balanced Accuracy (bacc) and F Beta Score (fbeta). An optimal ML model was constructed to predict three-year survival, and the predictive results were explained by SHapley Additive exPlanations (SHAP) framework. Meanwhile, 101 ML algorithm combinations were developed to select the best model with highest C-index to predict prognosis of pancreatic cancer patients.ResultsA total of 20,064 pancreatic cancer patients from SEER database was consecutively enrolled. We utilized eight clinical variables to establish prediction model for three-year survival. CatBoost model was selected as the best prediction model, and AUC was 0.932 [0.924, 0.939], 0.899 [0.873, 0.934] and 0.826 [0.735, 0.919] in training, internal test and external test sets, with 0.839 [0.831, 0.847] accuracy, 0.872 [0.858, 0.887] sensitivity, 0.803 [0.784, 0.825] specificity and 0.832 [0.821, 0.853] precision. Surgery type had the greatest effects on three-year survival according to SHAP results. For prognosis prediction, “RSF+GBM” algorithm was the best prognostic model with C-index of 0.774, 0.722 and 0.674 in training, internal test and external test sets.ConclusionsOur ML models demonstrate excellent accuracy and reliability, offering more precise personalized prognostic prediction to pancreatic cancer patients.

Abstract Tyrosine kinase inhibitors (TKIs) have revolutionized Philadelphia chromosome‐positive (Ph+) acute lymphoblastic leukemia (ALL) treatment. The combination of blinatumomab and a TKI in the frontline setting has shown the safety and efficacy of the chemotherapy‐free treatment approach in patients with Ph + ALL. This retrospective analysis included 19 patients with Ph + ALL and Ph‐like ALL treated with the combination of blinatumomab and a TKI. Of the 14 newly diagnosed patients, the overall response, complete remission (CR), and molecular response (CMR) rates after one cycle of blinatumomab were 100% (10/10), 90% (9/10), and 57% (8/14), respectively. Of the five relapsed patients, the CR and CMR rates were 50% (2/4) and 40% (2/5). Blinatumomab in combination with TKIs is safe and effective and hence this combination therapy could be a viable therapeutic option in front‐line treatment of patients with Ph + ALL.

IntroductionHigh engagement in physical education (PE) could effectively develop students’ motor competence and promote physical activity, which was significantly important for students’ physical and mental health. Researches had shown that motivation was an important factor in explaining students’ learning engagement, and variety-support as the fourth independent psychological need was a potential factor influencing students’ learning motivation. However, there was a lack of empirical research evidence on the effect of perceived variety-support on middle school students’ learning engagement in PE and the influencing mechanisms. This study aimed to investigate the direct effect of perceived variety-support on learning engagement in PE and the mediating effect of motivation in PE on the relationship.MethodsA cross-sectional study was conducted and 587 middle school students from Liaoning province filled the paper-and-pencil questionnaires adopting perceived variety-support in PE scale (PVSPES), utrecht work engagement scale-student (UWES-S), and perceived locus of causality in PE scale, which had been proved to have good reliability and validity (294 boys and 293 girls, Mage=13.47 ± 0.94).ResultsThe results showed three variables were significantly positively correlated with each other (r = 0.323-0.562 p

Abstract Background The eukaryotic-bacterial symbiotic system plays an important role in various physiological, developmental, and evolutionary processes. However, our current understanding is largely limited to multicellular eukaryotes without adequate consideration of diverse unicellular protists, including ciliates. Results To investigate the bacterial profiles associated with unicellular organisms, we collected 246 ciliate samples spanning the entire Ciliophora phylum and conducted single-cell based metagenome sequencing. This effort has yielded the most extensive collection of bacteria linked to unicellular protists to date. From this dataset, we identified 883 bacterial species capable of cohabiting with ciliates, unveiling the genomes of 116 novel bacterial cohabitants along with 7 novel archaeal cohabitants. Highlighting the intimate relationship between ciliates and their cohabitants, our study unveiled that over 90% of ciliates coexist with bacteria, with individual hosts fostering symbiotic relationships with multiple bacteria concurrently, resulting in the observation of seven distinct symbiotic patterns among bacteria. Our exploration of symbiotic mechanisms revealed the impact of host digestion on the intracellular diversity of cohabitants. Additionally, we identified the presence of eukaryotic-like proteins in bacteria as a potential contributing factor to their resistance against host digestion, thereby expanding their potential host range. Conclusions As the first large-scale analysis of prokaryotic associations with ciliate protists, this study provides a valuable resource for future research on eukaryotic-bacterial symbioses. Video Abstract

Abstract Background Predation is a fundamental mechanism for organisms to acquire energy, and various species have evolved diverse tools to enhance their hunting abilities. Among protozoan predators, raptorial Haptorian ciliates are particularly fascinating as they possess offensive extrusomes known as toxicysts, which are rapidly discharged upon prey contact. However, our understanding of the genetic processes and specific toxins involved in toxicyst formation and discharge is still limited. Results In this study, we investigated the predation strategies and subcellular structures of seven Haptoria ciliate species and obtained their genome sequences using single-cell sequencing technology. Comparative genomic analysis revealed distinct gene duplications related to membrane transport proteins and hydrolytic enzymes in Haptoria, which play a crucial role in the production and discharge of toxicysts. Transcriptomic analysis further confirmed the abundant expression of genes related to membrane transporters and cellular toxins in Haptoria compared to Trichostomatia. Notably, polyketide synthases (PKS) and l-amino acid oxidases (LAAO) were identified as potentially toxin genes that underwent extensive duplication events in Haptoria. Conclusions Our results shed light on the evolutionary and genomic adaptations of Haptorian ciliates for their predation strategies in evolution and provide insights into their toxic mechanisms.

Abstract As the predominant pest of alfalfa, Odontothrips loti Haliday causes great damages over the major alfalfa-growing regions of China. The characteristics of strong mobility and fecundity make them develop rapidly in the field and hard to be controlled. There is a shortage of bioinformation and limited genomic resources available of O. loti for us to develop novel pest management strategies. In this study, we constructed a chromosome-level reference genome assembly of O. loti with a genome size of 346.59 Mb and scaffold N50 length of 18.52 Mb, anchored onto 16 chromosomes and contained 20128 genes, of which 93.59% were functionally annotated. The results of 99.20% complete insecta_odb10 genes in BUSCO analysis, 91.11% short reads mapped to the ref-genome, and the consistent tendency among the thrips in the distribution of gene length reflects the quality of genome. Our study provided the first report of genome for the genus Odontothrips, which offers a genomic resource for further investigations on evolution and molecular biology of O. loti, contributing to pest management.

Abstract Background Aquaporins (AQPs) facilitate water diffusion across biological membranes and are involved in all phases of growth and development. Small and basic intrinsic proteins (SIPs) belong to the fourth subfamily of the plant AQPs. Although SIPs are widely present in higher plants, reports on SIPs are limited. Rice is one of the major food crops in the world, and water use is an important factor affecting rice growth and development; therefore, this study aimed to provide information relevant to the function and environmental response of the rice SIP gene family. Results The rice (Oryza sativa L. japonica) genome encodes two SIP-like genes, OsSIP1 and OsSIP2, whose products are predominantly located in the endoplasmic reticulum (ER) membrane but transient localization to the plasma membrane is not excluded. Heterologous expression in a yeast aquaglyceroporin-mutant fps1Δ showed that both OsSIP1 and OsSIP2 made the cell more sensitive to KCl, sorbitol and H2O2, indicating facilitated permeation of water and hydrogen peroxide. In addition, the yeast cells expressing OsSIP2 were unable to efflux the toxic methylamine taken up by the endogenous MEP permeases, but OsSIP1 showed subtle permeability to methylamine, suggesting that OsSIP1 may have a wider conducting pore than OsSIP2. Expression profiling in different rice tissues or organs revealed that OsSIP1 was expressed in all tissues tested, whereas OsSIP2 was preferentially expressed in anthers and weakly expressed in other tissues. Consistent with this, histochemical staining of tissues expressing the promoter-β-glucuronidase fusion genes revealed their tissue-specific expression profile. In rice seedlings, both OsSIPs were upregulated to varied levels under different stress conditions, including osmotic shock, high salinity, unfavorable temperature, redox challenge and pathogen attack, as well as by hormonal treatments such as GA, ABA, MeJA, SA. However, a reduced expression of both OsSIPs was observed under dehydration treatment. Conclusions Our results suggest that SIP-like aquaporins are not restricted to the ER membrane and are likely to be involved in unique membrane functions in substrate transport, growth and development, and environmental response.

Objective This study aimed to evaluate the effects of 2 endoscopic spine surgeries on the biomechanical properties of normal and osteoporotic spines. Methods Based on computed tomography images of a healthy adult volunteer, 6 finite element models were created. After validating the normal intact model, a concentrated force of 400 N and a moment of 7.5 Nm were exerted on the upper surface of L3 to simulate 6 physiological activities of the spine. Five types of indices were used to assess the biomechanical properties of the 6 models, range of motion (ROM), maximum displacement value, intervertebral disc stress, maximum stress value, and articular protrusion stress, and by combining them with finite element stress cloud. Results In normal and osteoporotic spines, there was no meaningful change in ROM or disc stress in the 2 surgical models for the 6 motion states. Model N1 (osteoporotic percutaneous transforaminal endoscopic discectomy model) showed a decrease in maximum displacement value of 20.28% in right lateral bending. Model M2 (unilateral biportal endoscopic model) increased maximum displacement values of 16.88% and 17.82% during left and right lateral bending, respectively. The maximum stress value of L4–5 increased by 11.72% for model M2 during left rotation. In addition, using the same surgical approach, ROM, maximum displacement values, disc stress, and maximum stress values were more significant in the osteoporotic model than in the normal model. Conclusion In both normal and osteoporotic spines, both surgical approaches were less disruptive to the physiologic structure of the spine. Furthermore, using the same endoscopic spine surgery, normal spine biomechanical properties are superior to osteoporotic spines.

Abstract Objective To enhance the detection, management and monitoring of Chinese children afflicted with sitosterolemia by examining the physical characteristics and genetic makeup of pediatric patients. Methods In this group, 26 children were diagnosed with sitosterolemia, 24 of whom underwent genetic analysis. Patient family medical history, physical symptoms, tests for liver function, lipid levels, standard blood tests, phytosterol levels, cardiac/carotid artery ultrasounds, fundus examinations, and treatment were collected. Results The majority (19, 73.1%) of the 26 patients exhibited xanthomas as the most prevalent manifestation. The second most common symptoms were joint pain (7, 26.9%) and stunted growth (4, 15.4%). Among the 24 (92.3%) patients whose genetics were analyzed, 16 (66.7%) harbored ABCG5 variants (type 2 sitosterolemia), and nearly one-third (8, 33.3%) harbored ABCG8 variants (type 1 sitosterolemia). Additionally, the most common pathogenic ABCG5 variant was c.1166G > A (p.Arg389His), which was found in 10 patients (66.7%). Further analysis did not indicate any significant differences in pathological traits among those carrying ABCG5 and ABCG8 variations (P > 0.05). Interestingly, there was a greater abundance of nonsense variations in ABCG5 than in ABCG8 (P = 0.09), and a greater frequency of splicing variations in ABCG8 than ABCG5 (P = 0.01). Following a change in diet or a combination of ezetimibe, the levels of cholesterol and low-density lipoprotein were markedly decreased compared to the levels reported before treatment. Conclusion Sitosterolemia should be considered for individuals presenting with xanthomas and increased cholesterol levels. Phytosterol testing and genetic analysis are important for early detection. Managing one’s diet and taking ezetimibe can well control blood lipids.

This study intends to determine the elements for adopting corporate social responsibility (CSR) practices that would boost the organization's performance (OP) either directly or indirectly in Pakistan's oil and gas sectors. This research demonstrates how companies may improve their performance by participating in various CSR efforts, for example, companies can encourage their workers by providing them with information about CSR practices, and they can support multiple facilitators of CSR integration to enhance OP.

The functional backfilling in mining achieves synergistic co-mining of deep-earth deposits-geothermal, and the development of functional filling materials with suitable phase change parameters and excellent physicochemical properties is imminent. This study aims to investigate the optimal composition of phase change energy storage backfill material based on MicroPCM. To address the issues of reduced compressive strength and specific heat capacity of MicroPCM-backfill, silicon carbide (SiC) is introduced to prepare MPSC-backfill. Box-Behnken Design (BBD) is proposed to design and analyze according to four factors (ash-sand ratio, slurry concentration, MicroPCM, SiC), three levels (compressive strength, thermal conductivity, specific heat capacity) and three center points. Based on the Response Surface Methodology, the multivariate quadratic polynomial model was used to perform multiple linear regression and binomial fitting analysis to explore the optimal ratio. Combined with SEM-EDS (Scanning Electron Microscope with Energy-Dispersive X-ray Spectroscopy) analysis, the following conclusions were drawn. SiC exhibits an overall enhancement of the compressive strength of the backfill, and the thermal conductivity tends to increase almost linearly. The specific heat capacity grows in a small amount of silicon carbide doping, but excessive amounts lead to a decrease, with an overall limited impact. The addition of SiC does not affect the phase change ability of MicroPCM. And within a certain range of added quantities, it optimizes specific heat capacity, thermal conductivity, and compressive strength. Experimental validation based on the obtained optimal composition shows errors within a 5% margin, indicating the response model's good accuracy and predictability. High and low-temperature cyclic tests confirm the long-term thermal energy storage stability of MPSC-backfill during phase change cycles.

This paper summarized the nursing management of an elderly long-term bedridden patient with purple urine bag syndrome. Given the association between purple urine bag syndrome and urinary tract infection, nurses had enhanced the interventions on catheter maintenance and urinary tract infection prevention. Meanwhile, comprehensive nursing interventions including diet guidance, constipation prevention and psychological care were carried out to relieve the clinical symptoms and negative emotions, and improve treatment adherence and recovery of the patient. (本文总结 1例高龄长期卧床男性患者并发紫色尿袋综合征(PUBS)的护理经验。PUBS的发生与尿路感染有关, 护理人员应加强导管维护和相关尿路感染护理, 同时给予饮食指导、便秘管理和心理护理, 改善患者便秘症状, 缓解负性情绪, 提高治疗依从性, 加快患者康复。)

Abstract Background Prader-Willi syndrome (PWS) is a rare genetic disease often associated with bone problems, mainly scoliosis and hip dysplasia (HD). This study aimed to analyze the clinical characteristics of orthopedic deformities in patients with PWS. Methods A retrospective study was conducted on 175 patients up to March 2023. The Cobb angle(CA) of the spine, the alpha angle of the hip joint, and the acetabular index (AI) were measured. This study aimed to evaluate the relationship between demographic parameters and bone deformities. Results Scoliosis was found in 66 patients (43.7%), including 52 (78.8%) with mild scoliosis, 10 (15.2%) with moderate scoliosis, and 4 (6.1%) with severe scoliosis. Only seven patients received orthopedic treatment (10.6%). The median age of scoliosis was 4.5 years old, and the prevalence of scoliosis increased rapidly at the age of 5 years and adolescence. The mean CA in this study increased gradually with age. HD was found in 47 patients (38.2%), and 6 patients received orthopedic treatment (12.7%). The median age at HD was 1.8 years old. The mean AI of the study population decreased with age. The prevalence of HD treated with recombinant human growth hormone (rhGH) was low. No significant differences were observed in sex, genotype, body mass index (BMI), obesity rate, or onset of scoliosis and HD. Conclusion The prevalence of scoliosis and HD was higher in patients with PWS. The onset age and developmental trends of the different skeletal malformations were different. Early diagnosis and treatment are important for the prognosis and treatment of orthopedic diseases in patients with PWS.

Abstract Objective To illustrate the status of all cancer clinical trials and characterize clinical trial enrollment disparities in the most common cancer. Methods Clinical trial data were extracted from ClinicalTrials.gov website. All searched clinical trials were included in the current status analysis of clinical trials on cancer. Among all the clinical trials, only trials addressing single disease sites of breast, prostate, colorectal, or lung (BPCRL) cancer were included in the age disparities analysis. The difference in median age (DMA) between the trial participant median age and the population-based disease-site-specific median age was calculated for each trial. Results A total of 7747 clinical trials were included in the current status analysis of clinical trials on cancer. The number of registered trials had been increasing from 2008 to 2021 (AAPC = 50.60, 95% CI 36.60, 66.00, P

The oil and gas exploration of the Middle and Lower Cambrian in the Tarim Basin reveals widely distributed source rocks with the Yuertusi Formation being recognized as high-quality source rocks that are distributed in a rather small range. The Xiaoerbulake Formation that is right under the Yuertusi Formation has also been eyed as potential high-quality source rocks and is studied through analyses focusing on the stratigraphic development, the abundance, type, and maturity of organic matter, and the paleoproductivity of a dark-colored algae dolomite within the formation. The results show that the dolomite is rich in organic matter of mainly types I and II kerogens. Although reached the high mature to over-mature stage, the dolomite was deposited in an anoxic sedimentary environment featuring a high paleoproductivity level and a high organic carbon burial efficiency, quite favorable for the development of high-quality source rocks. The study provides material evidence to the Middle-Lower Cambrian subsalt source rock-reservoir-caprock combination model for the Tarim Basin.

Abstract Background Blinatumomab early‐line treatment in B‐cell precursor acute lymphoblastic leukemia (B‐ALL) might improve clinical outcomes. Methods We conducted a retrospective real‐world cohort analysis in 20 newly diagnosed B‐ALL patients who received reduced‐dose chemotherapy (idarubicin, vindesine, and dexamethasone) for 1–3 weeks, followed by blinatumomab for 1–4 weeks as an induction therapy. Results At the end of the induction therapy, a complete remission rate of 100% was achieved; 17 (85%) patients were minimal residual disease (MRD) negative (

Abstract Background Gastric cardia adenocarcinoma (GCA) is classified as Siewert type II adenocarcinoma at the esophagogastric junction in Western countries. The majority of GCA patients do not exhibit early warning symptoms, leading to over 90% of diagnoses at an advanced stage, resulting in a grim prognosis, with less than a 20% 5‐year survival rate. Method Metabolic features of 276 GCA and 588 healthy controls were characterized through a widely‐targeted metabolomics by UPLC‐MS/MS analysis. This study encompasses a joint pathway analysis utilizing identified metabolites, survival analysis in both early and advanced stages, as well as high and negative and low expression of HER2 immunohistochemistry staining. Machine learning techniques and Cox regression models were employed to construct a diagnostic panel. Results A total of 25 differential metabolites were consistently identified in both discovery and validation sets based on criteria of p

Digital online application is a commonly used method for professional title evaluation in colleges and universities. With the rapid development of the mobile Internet of Things, the fastest way for users to access the evaluation system is through mobile devices. However, this also poses security challenges to the traditional professional title evaluation system. Therefore, this paper proposes a decentralized and security-enhanced digital title evaluation system in mobile IoT. Since the system uses blockchain to implement a decentralized review process, the transparency and fairness of the review process are guaranteed. At the same time, the review data and reviewer information in the system are encrypted and stored in the distributed network to ensure the security and non-tamperability of the data. All review records are recorded on the blockchain, and anyone can view and verify the legitimacy of the review results. Additionally, the system incorporates security enhancement mechanisms such as identity verification, smart contracts, and auditing functions to improve the credibility of the evaluation process and prevent cheating under mobile IoT. Experiments have shown that this system has important application value in the field of professional title review in universities, which can improve review efficiency, reduce human intervention, and provide strong support for the credibility of review results.

The aim of this study was to investigate the changes in the physical properties and gelation mechanism of xylooligosaccharides (XOS)-milk system during heat treatment. Firstly, the preparation conditions of XOS-milk gels were optimized using sensory evaluation. At the optimal XOS concentration, the changes in pH, color, gel strength, water-holding capacity, and rheological parameters of the XOS-milk system were studied during heat treatment. The gelation mechanism was analyzed by using solubility, particle size, and polyacrylamide gel electrophoresis analysis. The results showed that the XOS-milk gel was evaluated to be the highest sensory score at an XOS addition level of 5% (w/v) for 6 h of heat treatment. The pH of the XOS-milk system decreased with the increase of heating time (pH5.40 at 8 h), and the gel strength increased (144.4 g at 8 h). The system exhibited solid-like properties (G'>G'') after heat treatment for 2 h. The water-holding capacity initially increased (53.1% at 6 h) and then decreased (49.1% at 8 h) with prolonged heat treatment. Particle size and polyacrylamide gel electrophoresis analysis showed that milk proteins aggregated after heat treatment. The formation of gel network structure was observed using the scanning electron microscope (SEM). The solubility analysis showed that ionic bonds were the main driving force for protein aggregation and gel network formation, which contributed from 46.8% to 66.3% in samples of different heating time, while hydrophobic interactions and hydrogen bonds displayed the less contribution. As the heat treatment being prolonged, the total contribution of non-covalent bonds such as ionic bonds gradually decreased, which may be related to the formation of Maillard-related covalent bonds. Therefore, XOS promoted the formation of the heat-induced milk gel and its properties might have the potential to be used for the development of new milk products.

Abstract Novel prophylactic drugs and vaccination strategies for protection against influenza virus should induce specific effector T-cell immune responses in pulmonary airways and peripheral lymphoid organs. Designing approaches that promote T-cell-mediated responses and memory T-cell differentiation would strengthen host resistance to respiratory infectious diseases. The results of this study showed that pulmonary delivery of MENK via intranasal administration reduced viral titres, upregulated opioid receptor MOR and DOR, increased the proportions of T-cell subsets including CD8+ T cells, CD8+ TEM cells, NP/PA-effector CD8+ TEM cells in bronchoalveolar lavage fluid and lungs, and CD4+/CD8+ TCM cells in lymph nodes to protect mice against influenza viral challenge. Furthermore, we demonstrated that, on the 10th day of infection, the proportions of CD4+ TM and CD8+ TM cells were significantly increased, which meant that a stable TCM and TEM lineage was established in the early stage of influenza infection. Collectively, our data suggested that MENK administered intranasally, similar to the route of natural infection by influenza A virus, could exert antiviral activity through upregulating T-cell-mediated adaptive immune responses against influenza virus.

Polarization influences on the performance of multi-wavelength Brillouin Er3+-doped fiber laser are investigated by adjusting the polarization controller (PC) in the fiber loop mirror (FLM), where the linear laser cavity is composed of a fiber-tailed mirror and an FLM, and the stimulated Brillouin scattering (SBS) and the Er3+-doped fiber amplification (EDFA) simultaneously serve as the cavity gain. We realized 1–7 Brillouin laser lines by increasing the 980 nm pump power. For the first-order Brillouin laser, the signal–noise ratio (SNR) and optical intensity present a sinusoidal envelope; the conversion efficiency changes significantly from 0.56465 dBm/mw to 0.44975 dBm/mw by adjusting the ring’s angle in the PC; the first-order SBS thresholds are 20.4 mw, 36.1 mw and 28.5 mw at different angles θ2 = 36°, 276° and 300°, respectively; flatness between the two Brillouin lasers change obviously from 2.863 dB to 41.801 dB with different ring angles; the second-order Brillouin laser is suppressed and disappears finally at Δθ2 = −64° to −84° and 106°~136° angle variation. For the fifth-order Brillouin laser, the highest-order Brillouin laser line is seriously suppressed until it disappears at some angle variations similarly. The powers and wavelength stabilities for one-, three- and seven-wavelength Brillouin fiber lasers were measured in 1 h, and the obtained Er3+-doped multi-wavelength Brillouin fiber laser (MWBFL) worked stably during that time, but the stabilities become worse with higher SBS orders.

The quality of pump pulse in few-mode-fiber Brillouin optical time domain reflectometry (FMF-BOTDR) is vital for the spontaneous Brillouin scattering of modes LP01 and LP11 because it is the comprehensive effect of the main laser linewidth and pulse width, which is firstly discussed as we know. Numerical and experimental analysis are made for the amplitude and linewidth distribution, corresponding to the signal–noise ratio (SNR) and frequency resolution in BOTDR, respectively. Simulation shows the linewidths and peak values of Brillouin scattering have the same tendency for the LP01 mode and LP11 mode when the laser linewidth is less than 1 MHz but decreases slowly until they are the same when the laser linewidth is wider than 1 MHz. With the pulse width widening, the Brillouin linewidths for LP01 and LP11 modes both decrease sharply, almost to the natural linewidth of fiber 41 MHz and 35 MHz. Experimental results show that the amplitude distribution for the LP01 mode is always larger than for the LP11 mode if the main laser has the same linewidth and the frequency fluctuation is at least 2 MHz with the fiber laser and LP11 mode. The above results could provide improved sensing resolution for FMF-BOTDR sensing system.

Objective To study the expression of apoptosis related factors in the apoptosis pathway of death receptor in the pancreas of mice diabetic models. Methods The mice were divided into control group and model group. They were injected alloxan intraperitoneally twice (120 mg/kg,80 mg/kg). Blood glucose was measured on the third and seventh days after the model was established; Pancreatic tissue was taken for HE observation, TUNEL was used to detect the apoptosis rate, and RT-qPCR was used to detect the mRNA expression of tumor necrosis factor receptor 1 (TNFR1), Fas-related death domain (FADD), caspase-8 and caspase-3. Results Compared with the control group, pancreatic acinar cells in the model group showed granular degeneration, capillary congestion, decreased islet volume and the number of cells in islets. Compared with the control group, the apoptosis rate of pancreatic cells in the model group was significantly increased (P＜0.01). In addition, compared with the control group, the expression of Tnfr1, Fadd, caspase-8 and caspase-3 mRNA in the pancreas of the model group increased significantly or highly significantly on the third and seventh days (P＜0.05 or P＜0.01). Conclusions Alloxan could promote the over-expression of Tnfr1, Fadd, caspase-8 and caspase-3 mRNA in pancreatic tissue of diabetes model mice, and then induce apoptosis of pancreatic cells.

ObjectiveArgatroban is a highly promising drug for the treatment of acute ischemic stroke (AIS), but there is currently insufficient strong evidence regarding the efficacy and safety of using Argatroban in the treatment of AIS. Therefore, we conducted a systematic review and meta-analysis to evaluate the effectiveness and safety of Argatroban in the treatment of AIS.MethodsArticles on PubMed, Embase and the Cochrane Library databases were searched from these websites’ inceptions to 2th February 2023. Randomized controlled trials and observational studies on Argatroban therapy for acute ischemic stroke were included. Meta-analyses were conducted using a random-effects model.ResultsFourteen studies involving 10,315 patients were included in the meta-analysis. The results showed a significant reduction in the rate of early neurological deterioration (END) in the Argatroban group compared with the control group (OR = 0.47, 95% CI: 0.31–0.73, I2 = 15.17%). The rates of adverse events were no significant difference between the two groups (ICH: OR = 1.02, 95% CI: 0.68–1.51, I2 = 0.00%; major extracranial bleeding: OR = 1.22, 95% CI: 1.01–1.48, I2 = 0.00%; mortality: OR = 1.16, 95% CI: 0.84–1.59, I2 = 0.00%). However, the rates of mRS score of 0–1 (OR = 1.38, 95% CI: 0.71–2.67, I2 = 77.56%) and mRS score of 0–2 (OR = 1.18, 95% CI: 0.98–1.42, I2 = 0.00%) during the 90 days did not significantly improved in the Argatroban group. Subgroup analyses showed that the rate of END (OR = 0.41, 95% CI: 0.26–0.65, I2 = 2.77%) and mRS score of 0–2 (OR = 1.38, 95% CI: 1.06–1.81, I2 = 0.00%) had significantly improved when the intervention group adopted Argatroban plus Antiplatelet.ConclusionArgatroban can improve neurological deterioration, with a low incidence of adverse events such as bleeding and death, and general analysis showed no improvement in mRS. However, subgroup analysis suggests that compared to mono-antiplatelet therapy, combination therapy of Argatroban combined with antiplatelet therapy significantly reduced the incidence of END and improved mRS scores. After using Argatroban, there was no increase in the risk and mortality of intracranial hemorrhage and other bleeding sites.

BackgroundActual motor competence (AMC) and perceived motor competence (PMC), as determinants of children’s active participation in physical activity (PA), were important for children’s healthy development. The correlation between the two had been confirmed. To further understand this relationship, this study investigated the current status of AMC, PMC, and PA in Chinese school-age children, used a person-centered approach to reveal the characteristics of the development of motor competence (MC) in Chinese school-age children and the differences in the level of PA among different MC profiles of children.Materials and methodsA total of 532 children (age: M = 9.37, SD = 1.80 years-old) from grades 1 to 6 participated in this cross-sectional study (male, n = 284, 53.4%; female, n = 248, 46.6%). The Test of Gross Motor Development-3 (TGMD-3) was used to measure children’s AMC, the Pictorial Scale of Perceived Movement Skill Competence (PMSC) to measure children’s PMC, and the revised Chinese version of Physical Activity Questionnaire for Older Children (PAQ-C) to assess children’s PA levels.ResultsThere were some gender differences in AMC but no significant gender differences in PMC and PA in children. AMC and PA levels increased as the children aged, while PMC showed some decline. Cluster analysis identified four groups of children with different MC profiles. Two groups of children had corresponding AMC and PMC levels (the “high-high” cluster, N = 200, 38.91%; the “low-low” cluster, N = 63, 12.26%), and the other two groups were inconsistent in AMC and PMC (the “high-low” cluster, N = 100, 19.46%; the “low-high” cluster, N = 151, 29.38%). Significant differences in PA levels were found between children with different MC profiles. The “high-high” cluster children had the highest PA levels, whereas the “low-low” cluster children demonstrated the lowest PA levels.ConclusionAMC, PMC, and PA in Chinese school-age children were consistent with the pattern of child growth and development. Children with high AMC and high PMC usually had high levels of PA. Therefore, it was recommended to seize the best opportunity to intervene with children, and family, school, and community should synergize to help children improve AMC and PMC, and then actively participate in PA.

Abstract CD19 chimeric antigen receptor (CAR) T-cell therapy has shown great success against B-cell acute lymphoblastic leukemia (B-ALL). Tandem and sequential CD19/CD22 dual-target CAR T-cell therapies have been developed to reduce the possibility of CD19-negative relapse; however, the superior strategy is still uncertain. This study screened 219 patients with relapsed/refractory B-ALL who were enrolled in clinical trials of either CD19 (NCT03919240) or CD19/CD22 CAR T-cell therapy (NCT03614858). The complete remission (CR) rates in the single CD19, tandem CD19/CD22, and sequential CD19/CD22 groups were 83.0% (122/147), 98.0% (50/51), and 95.2% (20/21), respectively (single CD19 vs. tandem CD19/CD22, P = 0.006). Patients with high-risk factors achieved a higher rate of CR in the tandem CD19/CD22 group than in the single CD19 group (100.0% vs. 82.4%, P = 0.017). Tandem CD19/CD22 CAR T-cell therapy was one of the significant favorable factors in the multivariate analysis of the CR rate. The incidence of adverse events was similar among the three groups. Multivariable analysis in CR patients showed that a low frequency of relapse, a low tumor burden, minimal residual disease-negative CR and bridging to transplantation were independently associated with better leukemia-free survival. Our findings suggested that tandem CD19/CD22 CAR T-cell therapy obtains a better response than CD19 CAR T-cell therapy and a similar response to sequential CD19/CD22 CAR T-cell therapy.

Highlights Kevlar polyanionic chains induced the formation of highly ordered porous thermoplastic polyurethane foams with adjustable pore sizes. A tiny amount of Ti3C2Tx MXene was performed as reducing agent for the electroless deposition of copper nanoparticles. The conductive foam reinforced by aramid nanofibers exhibited excellent piezoresistive sensing and electromagnetic interference shielding performance.

Abstract Background Although the aberrant activation of NOTCH1 pathway causes a malignant progression of renal cell carcinoma (RCC), the precise molecular mechanisms behind the potential action of pro-oncogenic NOTCH1/HES1 axis remain elusive. Here, we examined the role of tumor suppressive miR-138–2 in the regulation of NOTCH1-HES1-mediated promotion of RCC. Methods This study employed bioinformatics, xenotransplant mouse models, ChIP assay, luciferase reporter assay, functional experiments, real-time PCR and Western blot analysis to explore the mechanisms of miR-138–2 in the regulation of NOTCH1-HES1-mediated promotion of RCC, and further explored miR-138–2-containing combination treatment strategies. Results There existed a positive correlation between down-regulation of miR-138 and the aberrant augmentation of NOTCH1/HES1 regulatory axis. Mechanistically, HES1 directly bound to miR-138–2 promoter region and thereby attenuated the transcription of miR-138-5p as well as miR-138–2-3p. Further analysis revealed that miR-138-5p as well as miR-138–2-3p synergistically impairs pro-oncogenic NOTCH1 pathway through the direct targeting of APH1A, MAML1 and NOTCH1. Conclusions Collectively, our current study strongly suggests that miR-138–2 acts as a novel epigenetic regulator of pro-oncogenic NOTCH1 pathway, and that the potential feedback regulatory loop composed of HES1, miR-138–2 and NOTCH1 contributes to the malignant development of RCC. From the clinical point of view, this feedback regulatory loop might be a promising therapeutic target to treat the patients with RCC.

Objective: Myomas of the uterus are the most common benign tumors of the female reproductive system. This article presents a detailed review of the suturing methods and ancillary techniques for uterine incision used in transumbilical single-port laparoscopic myomectomy in order to provide a reference for beginners and a research direction for the future development of this surgery. Mechanism: The authors reviewed credible search engines and literature databases (such as PubMed) for the period 2000–2023, extracted published data and content, and summarized the collected information. This was combined with the authors own surgical experience to provide a detailed description of the suture methods and ancillary techniques for uterine incision used in transumbilical single-port laparoscopic myomectomy. Findings in Brief: This review provides a comprehensive understanding of the suture methods and ancillary techniques for uterine incision commonly used in transumbilical single-port laparoscopic myomectomy. The advantages and limitations of different methods are also recognized. Conclusions: Suture methods for uterine incision in transumbilical single-port laparoscopic myomectomy include: simple interrupted suture, figure-of-eight suture, interrupted mattress suture, simple continuous suture and baseball type suture. Ancillary techniques for uterine incision in transumbilical single-port laparoscopic myomectomy include: needle hook assisted method, suspended line method, one-handed operation method and the modified operative method.

In the culture of crustaceans, most species show sexual dimorphism. Monosex culture is an effective approach to achieve high yield and economic value, especially for decapods of high value. Previous studies have developed some sex control strategies such as manual segregation, manipulation of male androgenic gland and knockdown of the male sexual differentiation switch gene encoding insulin-like androgenic gland hormone (IAG) in decapods. However, these methods could not generate hereditable changes. Genetic manipulation to achieve sex reversal individuals is absent up to now. In the present study, the gene encoding IAG (EcIAG) was identified in the ridgetail white prawn Exopalaemon carinicauda. Sequence analysis showed that EcIAG encoded conserved amino acid structure like IAGs in other decapod species. CRISPR/Cas9-mediated genome editing technology was used to knock out EcIAG. Two sgRNAs targeting the second exon of EcIAG were designed and microinjected into the prawn zygotes or the embryos at the first cleavage with commercial Cas9 protein. EcIAG in three genetic males was knocked out in both chromosome sets, which successfully generated sex reversal and phenotypic female characters. The results suggest that CRISPR/Cas9-mediated genome editing technology is an effective way to develop sex manipulation technology and contribute to monosex aquaculture in crustaceans.

Background Chronic obstructive pulmonary disease (COPD) is considered related to chronic systemic inflammation. Renin angiotensin system (RAS) inhibitor, exerting an anti-inflammatory action in many systems, has been demonstrated relevant to the pathogenesis of COPD. However, the association between RAS inhibitor use and prognosis of patients with COPD remains controversial. Therefore, we conducted a meta-analysis and systematic review to summarise current evidence.Material and methods Databases, including Medline, Embase, Web of Science and Cochran Library, were searched for eligible studies by the end of 30 September 2022. Observational studies or randomised controlled trials (RCTs) that investigated the association of RAS inhibitor use with prognosis of COPD (mortality or risk of acute exacerbation) were selected. The Newcastle-Ottawa Scale was used for quality assessment of observational studies, while the Cochrane risk-of-bias tool was used to assess the quality of RCTs. Statistical analyses were performed using Stata V.15. We selected relative risk (RR) with 95% CI as the effect measure. Heterogeneity was assessed by I-squared (I2) statistics. The funnel plot was used for visual assessment of publication bias.Results A total of 20 studies with 5 51 649 subjects were included in the meta-analysis. The overall analysis indicated that RAS inhibitor use decreased the risk of death in patients with COPD (RR: 0.69, 95% CI: 0.61 to 0.78). Subgroup analyses were conducted according to comorbidities, race and type of RAS inhibitors, and the results kept consistent. However, in the pooled analysis of prospective studies, RAS inhibitor use did not significantly decrease the mortality (RR: 0.89, 95% CI: 0.78 to 1.02). Additionally, the risk of exacerbations of COPD did not decrease in patients who were prescribed RAS inhibitors (RR: 0.99, 95% CI: 0.80 to 1.23). The funnel plot indicated significant publication bias.Conclusion RAS inhibitor use seemed to be associated with a reduction of mortality in patients with COPD. However, the available evidence is weak due to potential biases from retrospective studies and the heterogeneity across included studies.

This study examines the impact of “workplace environment and reward systems on motivation and employee performance.” It also examines the mediating role of motivation and moderating role of communication in the telecom sector of Karachi, Pakistan. Based on the self-administered questionnaire, we collected 418 responses from the telecom sector employees. This research is quantitative, and the approach is deductive. Using Smart PLS for analysis, we found workplace environment and reward systems affect motivation. The workplace environment and reward system promote employee performance. Motivation has a mediating effect on (i) the workplace environment and employees’ performance and (ii) the reward system and employee performance. The study did not find support for the moderating effect of internal communication on motivation and employee performance.

Objectives Detailed analysis targeting B cell subgroups was considered crucial in monitoring autoimmune diseases and treatment responses. Thus, precisely describing the phenotypes of B cell differentiation and their variation in primary Sjögren’s syndrome (pSS) is particularly needed. Methods To characterize the proportions and absolute counts of B cell subsets, peripheral blood from 114 healthy adults of China (age range: 19–73 years) and 55 patients with pSS were performed by flow cytometry and CD19, CD20, CD24, CD27, CD38 and IgD were used as surface markers to identify B cell mature process. Age- and gender-stratified analyses were then carried out to improve the interpretation of B cell subsets. Results The assessments from healthy adults showed that the proportion of naive B cells presented a significant increase with age. A reversal trend was noted that the percentage of B10 decreased markedly with age. In addition, analysis based on gender showed that the relative percentage and number of naive B cells were higher in females than in males whereas the proportions of switched memory B cells and B10 cells were decreased in female. Patients with pSS exhibited a significant expansion in naïve B cells and unswitched memory B cells, accompanied with decreased switched memory B cells and B10 cells, which were identified to be associated with autoantibody production. Conclusions Our study presented a reliable analysis by flow cytometry to cover the principal B cell subtypes. These different stages of B lymphocytes may have implications for evaluating the activation of pSS and other autoimmune diseases and treatment efficacy.KEY MESSAGES B cell subsets play a pivotal role in the pathogenesis of primary Sjögren’s syndrome (pSS) and other autoimmune diseases. A practical and accurate flow cytometry method to profile B cell phenotypes in peripheral blood of healthy adults is especially essential. Additionally, we presented reliable reference ranges for B cell subsets in regards to the local population. Age- and gender-related analyses are available to better understand their influence in immune status and treatment outcome. The distribution of B-cell subsets is found substantially altered in patients with pSS, bringing novel avenues for pSS research in the future.

ObjectivesIn this study, we compared the cost-effectiveness comparison of the active surveillance (AS) and early surgery (ES) approaches for papillary thyroid microcarcinoma (PTMC) from the perspective of the Chinese healthcare system.MethodsWe performed a cost-effectiveness analysis using a Markov model of PTMC we developed to evaluate the incremental cost-effectiveness ratio of AS and ES. Our reference case was of a 40-year-old woman diagnosed with unifocal (

Micro-organic contaminants in water have the characteristics of relatively low concentration and degradation, given their stable structures. Ecological balance and human metabolism can be severely affected by their accumulation in the food chain. Besides, extensive human activities and industrialization have accelerated their transmission and accumulation in the environment. So there is an urgent need to treat micro-organic contaminants in water. This review focused on the methods and mechanisms of removing micro-organic contaminants, including biodegradation, advanced oxidation, adsorption, and membrane separation. Notably, the application of membrane separation and its integrated technology in removing micro-organic contaminants, which is less covered in the previous studies. This review also introduced the previous study of developing modified ultrafiltration membranes for simultaneously removing both large- and small-molecule contaminants. These modified ultrafiltration membranes can be applied to remove micro-organic contaminants and are expected to broaden the application of ultrafiltration.

Abstract This study prospectively compared the efficacy and safety between matched related donor-hematopoietic stem cell transplantation (MRD-HSCT) (n = 108) and immunosuppressive therapy (IST) plus eltrombopag (EPAG) (IST + EPAG) (n = 104) to determine whether MRD-HSCT was still superior as a front-line treatment for patients with severe aplastic anemia (SAA). Compared with IST + EPAG group, patients in the MRD-HSCT achieved faster transfusion independence, absolute neutrophil count ≥ 1.0 × 109/L (P

Abstract Background This study compared the survival outcomes of different surgical approaches to determine the optimal approach for gastric cardia adenocarcinoma (GCA) and aimed to standardize the surgical treatment guidelines for GCA. Methods A total of 7103 patients with GCA were enrolled from our previously established gastric cardia and esophageal carcinoma databases. In our database, when the epicenter of the tumor was at or within 2 cm distally from the esophagogastric junction, the adenocarcinoma was considered to originate from the cardia and was considered a Siewert type 2 cancer. The main criteria for the enrolled patients included treatment with radical surgery, no radio- or chemotherapy before the operation, and detailed clinicopathological information. Follow-up was mainly performed by telephone or through home interviews. According to the medical records, the surgical approaches included transthoracic, thoracoabdominal, and transabdominal approaches. Kaplan–Meier and Cox proportional hazards regression models were applied to correlate the surgical approach with survival in patients with GCA. Results There were marked differences in age and tumor stage among the patients who underwent the three surgical approaches (P