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This investigation was aimed to assess and evaluate the effect of frying process with three different oils (corn, olive and margarine) on fatty acids and lipid classes’ composition of clam and their impact on the nutritional value and tissues quality. The results indicated that the contents of saturated, monounsaturated and polyunsaturated fatty acids varied significantly in fried clams and frying oil. Our data demonstrated that all lipid classes’ contents increased significantly after all frying processes. It was concluded that the frying process decreased the n-3/n-6 and DHA+EPA and increased the levels of atherogenic; thrombogenic indices. Additionally, these culinary processes promoted lipid peroxidation through the enhancement of TBARs, PV and FFA. These changes were more significant and intensive after frying with margarine and corn oils followed by virgin olive oil. These results suggest that clam fried with olive oil can be classified as highly nutritive in regard to its lipid content, major fatty acid profile; and nutritional value.

In order to increase the low-temperature resistance of Nile tilapia, the purpose of this study was to determine the potential effect of ω3 fatty acid incorporation in Oreochromis niloticus diet. To perform this, two experimental diets containing soybean oil (D1) and cod liver oil (D2) have been supplied to juvenile tilapia for 30 days. According to our results, similar improvements in the two diets have been recorded for growth performance of O. niloticus including the final body mass, specific growth rate, and feed conversion ratio. Our results showed that fish fed with diet D2 promoted high polyunsaturated fatty acids mainly n-3 series (PUFA (n-3)) percent, highlighting the increased levels of docosahexaenoic (DHA) and eicosapentaenoic (EPA) as well as the activation of their conversion enzyme ratios D5D and D6D desaturases. The second objective was to assess the effect of the two experimental diets on low water temperature tolerance. This was done by exposing juvenile fish at the end of the first experiment to 16, 14, 12, 10, and 8 °C for 12 h, 24 h, and 48 h. The sub-lethal LT

The effect of storage time (5, 10 and 15 days) and temperature (− 20 °C and + 4 °C) on the biochemical composition of the Tunisian mussel (Mytilus galloprovincialis) was evaluated by investigating changes in proximate composition, fatty acids, minerals and nutritional quality indices. Lipid and protein degradations were also evaluated through several markers of oxidative damage. Results showed a significant decrease in the biochemical compounds in mussel tissues after both refrigeration and freezing processes (p

Biochemical composition and fatty acid profile of raw Ark shells (RA) were compared to Ark shells submitted to three different cooking methods (BA: baking in the oven; PF: pan-frying in butter and MW: cooking in a microwave). Moisture (%) was significantly higher in RA (79.66) with respect to PF (65.09), BA (48.63) and MW (47.02). Protein (mg/g of flesh) decreased significantly from 18.62 in RA to 15.40 in MW, 13.76 in PF and 13.33 in BA. However, lipids significantly increased in MW (43.32 mg/g of flesh) and PF (63.63 mg/g of flesh) with respect to RA (35.05 mg/g of flesh). Pan-frying affected considerably triacylglycerol (TAG) and the fatty acid composition (FA) of Ark shell flesh. The most changes occurred in saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acid fractions after this process. The n-3 PUFA decreased significantly from RA (16.40 mg/g dry weight) to PF (10.02 mg/g DW). While, the opposite trend was observed for n-6 PUFA, revealing that this cooking method had considerable effects on the nutritional characteristics of this edible shellfish. The analysis of lipid peroxidation markers such as thiobarbituric acid reactive substances, free fatty acid and peroxide value confirmed that both heat treatment and time of cooking caused lipid degradation, which had been more accentuated during pan-frying treatment. For the populations who consume Ark shells occasionally or frequently, baking and microwave cooking could be then considered as wiser and healthier cooking methods since they conserve better the nutritional value of this marine product. The present study will be of practical value from a health perspective for Mediterranean populations.

The aim of this study was to analyze the biochemical alterations in the gills of Venus verrucosa under exposure for 6 days to three doses of lambda-cyhalothrin (λ-cyh) (100, 250, and 500 µg L−1). Malondialdehyde, lipid hydroperoxide, and hydrogen peroxide levels in the gills of treated groups increased. λ-Cyh exposure significantly increased the protein carbonyl and reduced glutathione levels in the gills of all treated groups. Moreover, the activities of superoxide dismutase and glutathione peroxidase were increased. In our study, the polyunsaturated fatty acid (FA), omega-6, eicosapentaenoic acid (C20:5n-3), and docosahexaenoic acid (C22:6n-3) were increased in the treated groups. A significant decrease in the saturated FAs, omega-3, and arachidonic acid (C20:4n-6) levels was observed. The content of monounsaturated FA was changed in the groups treated with 100 and 250 µg L−1of λ-cyh. As a corollary, desaturase and elongase activities were significantly increased. Our study provides evidence of the underlying toxic mechanism of λ-cyh and its capacity to create oxidative stress and revealed that FA profiling is a new approach for elucidating the λ-cyh toxicity.

Lead (Pb) is one of the most toxic heavy metals that affect the physiological status of aquatic organisms. The present investigation evaluated the possible toxic effect of lead chloride (PbCl2) on ...

Zinc is one of the important essential trace minerals to human health due to its antioxidant properties. The present study was conducted to elucidate its potential protective role against maneb-induced nephrotoxicity. For this purpose, animals were randomly divided into four groups of six each. Mice of group I (negative controls) have received daily 0.5 ml of distilled water, a solvent of maneb. Mice of group II (MB) have received 30 mg/kg bw of maneb daily by intraperitoneal way. Mice of group III (MB + Zn) have received the same dose of maneb as group II, along with ZnSO4 (30 mg/kg bw) daily. Mice of group IV (Zn), considered as positive controls, have received the same dose of ZnSO4 as group III daily. Our results revealed that ZnSO4 co-administration to maneb-treated mice decreased kidney levels of malondialdehyde, hydrogen peroxide, protein carbonyls, and advanced oxidation protein products; the levels of non-enzymatic antioxidants like vitamin C, glutathione, and metallothionein. It recovered the alteration of antioxidant enzyme activities (catalase, superoxide dismutase, and glutathione peroxidase) and attenuated DNA fragmentation. Furthermore, this essential trace element was also able to alleviate kidney biomarkers’ alterations by lowering plasma levels of creatinine, urea, uric acid, and lactate dehydrogenase. In addition, the histopathological changes induced by maneb were improved following zinc administration. Our results indicated that zinc might be beneficial against maneb-induced renal oxidative damage in mice.

The present work aims to determine and compare the tolerance to different stress environmental conditions in two crabs’ species the native Carcinus aestuarii and the invasive Portunus segnis. In this study, C.aestuarii appeared more intolerant to environmental stressor as shown by the important changes occurred on the proximate, fatty acids and their essential compounds comparing to P.segnis. Our results revealed accumulation of metallic trace elements, macromolecular damages and antioxidant defense systems perturbations mostly in C.aesuarii gills and muscles than P.segnis, thus highlighting the importance of a multi-markers approach to assess the urban pollution in coastal ecosystems. According to this study, fatty acid profiles and oxidative stress biomarker examination of both crabs indicate that the different environmental stresses significantly influence the tolerance of C.aestuarii compared to the invasive P.segnis which may provide physiological advantages for the achievement of their invasion in novel ecosystems.

Narcissus tazetta (Amaryllidaceae) is a medicinal plant, widely used for cut flowers and potted ornamental plant in Tunisia flora. The current study evaluated the phenolic composition and antioxidant properties of its flower extracts and investigated its potential protective activity against cadmium chloride (CdCl2)-induced hepatotoxicity in mice. Adult Swiss mice were exposed either to CdCl2 (0.16 mg/kg bw) or to Narcissus tazetta extract (100 or mg/kg bw) or to their combination. Cadmium chloride and Narcissus tazetta flower extracts were given by intraperitoneal injection and gavage respectively for eight consecutive days. Results indicated that ethanolic flower extracts contained high levels of total phenolic and flavonoid contents along with a strong total antioxidant and 2,2-Diphenyl-1-picrylhydrazyl free radical scavenging activities. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis identified eight phenolic compounds, including rutin, Kaempferol glycosides and chlorogenic acids. The extract also exhibited marked hepato-protective effects against CdCl2 toxicity by reducing hepatic levels of malondialdehyde, advanced oxidation protein products, hydrogen peroxide, metallothioneins and DNA degradation. Additionally, co-administration of Narcissus tazetta flower extracts lowered the plasma activities of transaminases, gamma glutamyl transpeptidase and lactate dehydrogenase, and increased hepatic levels of reduced glutathione, non-protein thiols, vitamin C, and catalase activity. The hepatoprotective effects of the extract were demonstrated by histopathological improvement of liver disorders. The current study provided ethnopharmacological application of Narcissus tazetta flower extracts against CdCl2 induced oxidative stress, suggesting its chemoprevention role of its phenolic compounds as a natural antioxidant.

The present study aimed to evaluate the biochemical status of Venerupis decussata collected seasonally from two Tunisian coastline lagoons (Ghar El Melh and Boughrara) submitted to different anthropogenic levels. Trace elements were significantly higher in clams from Boughrara, especially during the warm season. Fatty acids, proximate compositions, and nutritional value of both clam populations were higher, appearing to be the best for human consumption principally during the spring season. The principal component analysis was determined, including phospholipids and reserve lipids, revealing a great variation with the exogenous and endogenous factors. Generally, clams from Boughrara had a significant alteration in biochemical composition principally due to the anthropogenic levels and physicochemical fluctuation of this lagoon. Our results reflected the healthy benefit of clam consumption and the use of energy reserves and essential fatty acids as a great biomarker to assess ecological risk in lagoons and other coastal ecosystems.

Mercury is one of the most harmful pollutant that threat marine biota. This study assessed the Hg impact on the fatty acid (FA) composition and the antioxidant statues in Holothuria forskali body wall tissue. Specimens were exposed to HgCl2 graded doses (40, 80 and 160 μg L−1) for 96 h. A decrease in linoleic, arachidonic and eicosapentaenoic acid levels and an increase of docosahexaenoic acid were mainly observed at the nominal tested dose. The exposure to the upper dose promoted oxidative stress with an increase of malondialdehyde, hydrogen peroxide, advanced oxidation protein product, glutathione and non-protein thiols levels. Moreover, a decrease in catalase and an increase in superoxide dismutase and glutathione peroxidase activities were observed. Yet, an increase of the metallothionein level was registered in all treated groups. This study confirmed the Hg toxicity on the redox statue of H. forskali and highlighted the usefulness of the FA composition as an early sensitive bioindicators.

Mercury (Hg) is among the most deleterious contaminant in the aquatic environment and presents a serious risk to humans and ecosystems. This study evaluated the effects of Hg on oxidative stress bi...

Narcissus tazetta (Amaryllidaceae) is a medicinal plant widely used for cut flowers and potted ornamental plant in Tunisia flora. The current study evaluated the phenolic composition and antioxidant properties of its flower extracts and investigated its potential protective activity against cadmium chloride (CdCl

3 Figuras.-- 5 Tablas, Narcissus tazetta (Amaryllidaceae) is a medicinal plant widely used for cut flowers and potted ornamental plant in Tunisia flora. The current study evaluated the phenolic composition and antioxidant properties of its flower extracts and investigated its potential protective activity against cadmium chloride (CdCl)–induced hepatotoxicity in mice. Mice were divided into six groups of six each: group 1, serving as negative controls, received by intraperitoneal way only distilled water; group 2 received by intraperitoneal way CdCl (0.16 mg/kg bw); groups 3 and 4 received CdCl at the same dose of group 2 and 100 or 200 mg/kg bw of Narcissus tazetta flower extracts via oral route; groups 5 and 6, serving as positive controls, received only Narcissus tazetta flower extracts. Polyphenolic compounds of the extract were analyzed by colorimetric and high-performance liquid chromatography-mass spectrometry (HPLC-MS) methods. Total antioxidant activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging potential of the extract were estimated using colorimetric method. Results indicated that ethanolic flower extract contained high levels of total phenolic and flavonoid along with a strong total antioxidant and DPPH free radical scavenging activities. HPLC-MS analysis identified eight phenolic compounds, including rutin, kaempferol glycosides, and chlorogenic acids. The extract also exhibited marked hepatoprotective effects against CdCl toxicity by reducing hepatic levels of malondialdehyde, advanced oxidation protein products, hydrogen peroxide, metallothioneins, and DNA degradation. Additionally, co-administration of Narcissus tazetta flower extracts lowered the plasma activities of transaminases, gamma glutamyl transpeptidase, and lactate dehydrogenase and increased hepatic levels of reduced glutathione, nonprotein thiols, vitamin C, and catalase activity. The hepatoprotective effects of the extract were demonstrated by histopathological improvement of liver disorders. The current study provided ethnopharmacological application of Narcissus tazetta flower extracts against CdCl-induced oxidative stress suggesting its chemoprevention role of its phenolic compounds as a natural antioxidant., This work received financial assistance from the Ministry of Higher Education and Scientific Research of Tunisia

Coastal lagoons are among the most vulnerable ecosystems as they are often exposed to different anthropogenic activities. The Polychaetes, which are dominant components in macrobenthic community, are particularly exposed to contamination. The current study was designed to assess and compare the sensitivity of different polychaetes species towards urban pollution. To do this, three polychaete species: Perinereis cultrifera, Diopatra neapolitana, and Marphysa sanguinea, were collected from the Tunis South Lagoon during summer 2013. A set of biomarkers indicative of genotoxicity (DNA damage), biotransformation, and oxidative stress (glutathione S-transferase, GST) as well as immune response (cyclooxygenase activity (COX), lysozyme activity, and nitric oxide level (NOx)), was used. The results revealed that D. neapolitana and P. cultrifera exhibited higher genetic alteration and GST activity and more prominent immune response when compared with M. sanguinea. These findings denote of the higher sensitivity of D. neapolitana and P. cultrifera to urban pollution and suggest their possible use in environmental biomonitoring programs.

In this study, the potential hazardous impacts of the technical grade glyphosate acid (GLY) and its commercial formulation roundup (RD®) were evaluated for the first time on holothurians. To do this, redox status, fatty acid (FA) profile, and histopathology aspects were assessed in the respiratory tree tissue of the sea cucumber Holothuria forskali following short-term exposure (96 h) to a series of concentrations (10, 100 and 1000 μg L−1) of GLY and RD® (glyphosate acid equivalent). Our results showed that both GLY and RD® promoted oxidative stress highlighted with an increase of hydrogen peroxide (H2O2), malondialdehyde (MDA), lipid peroxides (LOOH) and advanced oxidation protein product (AOPP) levels in all treated groups. In addition, both glyphosate forms were found to perturb the FA composition. However, changes in saturated (SFA) and polyunsaturated (PUFA) including some essential FA (LA, ARA, EPA and DHA) revealed differential compensatory/adaptive processes in H. forskali depending on the treatment. GLY and RD® were also found to modulate the enzymatic (glutathione S-transferases, glutathione peroxidase and catalase) and non-enzymatic (reduced glutathione and ascorbic acid) antioxidant defense status. Taken together, our results revealed that the commercial formulation produced more pronounced effects on H. forskali respiratory tree than the pure form. This finding was further confirmed by the histological observations.

The present study assessed the consequences of environmental changes on the biochemical quality and the physiological condition of the whole body and others organs Mactra stultorum collected from the north coast of Tunisia. Significant variations in the biochemical components, PE and CI have shown between seasons. The depletion of glycogen in all tissues was recorded during spawning periods (late spring and summer). Lipid accumulation in gonad–visceral mass during the early and late gametogenesis stages was associated with the increase in CI and GSI. However, decreases in lipid and protein contents were recorded in all the tested organs during summer when the animals are in the spawning period, suggesting that clams could accumulate proteins and lipids. Regarding the environmental factors, a negative and a significant correlation was recorded between glycogen, lipid contents and water temperature. Based on these results and as M. stultorum is appreciated by the consumers, we suggest that the harvesting of M. stultorum should be concentrated on February to June when the whole body was characterized by an important amount of the biochemical composition when the seafood is at its highest nutritive value as compared to August to January. Because of its high nutritive value especially it’s richness by some important and essentials fatty acids, M. stultorum seems to be considered as an important commercial species in many countries and in Tunisia particularly. We suggest that harvesting of M. stultorum should be concentrated on the period when the seafood is at its highest nutritive value. survivability rates from malignancy. The greatest variations in the mortality ratios existed within the European countries.

Coastal transitional waters are exposed to many anthropogenic threats. This study aims to assess the trace metals' pollution status of transitional waters by evaluating its biological effects in the clam Venerupis decussata. Among the studied sites along the Tunisian littoral, South Tunis and Boughrara were the most impacted, since clams from these two lagoons presented significant differences in: (i) trace metal contents, (ii) in-cell hydrogen peroxide, (iii) enzymatic and non-enzymatic defenses, (iv) damage to lipids and proteins, and (v) protein post-translational modifications. These changes related to evident histopathological traits. PCA showed a clear separation between the digestive gland and gills tissues and illustrated an impact gradient in Tunisian coastal lagoons. Water temperature was revealed as an added natural stressor that, when concurring with high pollution, may jeopardize an ecosystem's health and contribute to the accumulation of hazardous metals in organisms., This study was supported by the Spanish Ministry of Economy and Competitiveness -CTM2016-75908-R-, by the Andalusian Plan of Research, Development, and Innovation, and by the University of Cordoba “Plan Propio” program to group BIO187 (Spain). Safa Bejaoui stay in Cordoba was funded by the Tunisian government, Ministry of Higher Education and Scientific Research, University of Tunis EL Manar, Laboratory of Ecology, Biology and Physiology of Aquatic Organisms -EBPAO, LR18ES41- (Tunisia). Pedro M. Costa acknowledges Fundação para a Ciência e Tecnologia -FCT-MCES- for the grant IF/00265/2015 and for the support to UCIBIO, Applied Molecular Biosciences Unit -UID/Multi/04378/2019- (Portugal).

Acrylamide (ACR) is among the most deleterious pollutants in the environment and presents a serious risk to humans and ecosystems. The purpose of this study was to assess its effects when administered at different concentrations (5, 10 and 20 mg L–1) to evaluate antioxidant status in the gills of Mactra stultorum. Our results showed, after five days of treat­ment, an increase in malondialdehyde (MDA), lipid hydroperoxides (LOOH), advanced oxidation protein products (AOPP), reduced glutathione (GSH), ascorbic acid (Vit C) and metallothionein (MDA) levels in gills of treated clams compared with controls. Moreover, an increase in superoxide dismutase (SOD) and a significant decrease in glutathione peroxidase (GPx) activities were also observed. Acrylamide induced neurotoxicity, as evidenced by the inhibition of acetylcholinesterase (AChE) activity in a dose-dependent manner. Overall, our results indicated that oxidative stress may be considered one of the mechanisms behind acrylamide toxicity in bivalves, although the subject requires more research.

Monthly variations of fatty acid composition of the two fractions polar lipids (PL) and neutral lipids (NL) in the adductor muscle of cupped oyster Crassostrea gigas, were studied from May 2005 until June 2006 in the Bizert lagoon, related to environmental parameters and reproductive events. C. gigas showed a clear cycle of energy storage and utilization in the site of study. Total PL and NL content varied significantly during the year and showed a negative and significant correlation with the gametogenic condition index which can be explained by transfer of fatty acids from the adductor muscle to gonads to support the gametogenic cycle. Nevertheless, PL and NL have no significant correlations either with temperature or chlorophyll a registered in the site of study. The adductor muscle proved to be less influenced by chlorophyll a richness of the farming environment. The dominance of total polyunsaturated fatty acids (PUFAs) over the saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in both PL and NL fractions was established throughout the period of study. Oysters analyzed were characterized by high levels of PUFAs n−3 and high n−3/n−6 PUFAs ratio in both PL and NL fractions. PUFAs n−3 and SFAs of PL and PUFAs and SFAs of NL, showed a negative and significant correlation between them. In fact, low temperature values produce generally an increase in the PUFAs percentages in order to maintain the fluidity of cell membranes. We deduce that annual variations of fatty acids content in PL and NL fractions of the adductor muscle of C. gigas seem to be in relation with the reproductive events. According to our results, the adductor muscle tissues were relatively poor in fatty acids (maximum values (% of dry weight) of PL and NL respectively were 2.04 ± 0.33 and 1.75 ± 0.9). Lipids fluctuations were not pronounced all over the period of study in the site of sampling due to low effects of the environment richness and phases of the sexual cycle which aspires the adductor muscle contents of fatty acids to support other organs in the gametogenic process. But this organ arrives despite its difficulties to maintain a high level of n−3/n−6 PUFA and having a good nutritional quality.

Bivalves are known for their high nutritional value and are considered a healthy food for human consumption. The effect of different cooking methods (steaming, boiling, grilling, and frying) on pro...

The aim of this work was to investigate the influence of two different drying methods, traditionally by sun and controlled temperatures of 45, 50, 60 and 70 °C and relative humidity of 20 and 30% on the chemical composition of Sepia officinalis. When compared to fresh samples, lipid content increased under both drying methods whereas protein levels significantly increased only under controlled conditions. Drying procedures increased the percentage of saturated fatty acids mainly in sun-dried samples and at high temperature (70 °C). A significant decrease in the amount of polyunsaturated fatty acids was detected in sun-dried samples. No significant differences were found between the levels of polyunsaturated fatty acids of fresh cuttlefish and those under controlled conditions. The highest value of malondialdehyde in mantles was observed in sun-dried samples, while in tentacles the highest level was seen at 70 °C. The highest level of advanced oxidation protein products was noted in traditionally sun-dried samples. The results obtained showed that 45–50 °C and 20–30% humidity conserved the value of proteins and lipids more than traditional drying. Results of this study may be suitable for developing a reliable industrial index.

The invasive Portunus segnis, an atlantic species, was recently recorded in Tunisian waters. Since its record in the Gulf of Gabes, no studies have been carried out about the biochemical analysis of this alien species. In this context, our study aims to evaluate the fatty acid profile, lipid classes (neutral and polar), and nutritional value of breast of this invasive species. Specimens sampled from the gulf of Gabes were found to be rich in protein and oil, with significant differences between sexes. The level of saturated, monounsaturated, and polyunsaturated fatty acids in neutral and polar showed a significant difference between sexes. Saturated fatty acids were significantly higher in male P. segnis compared to the female. Docosahexaenoic (DHA), eicosapentaenoic (EPA), and arachidonic (ARA) were the most predominate polyunsaturated fatty acids (PUFA) in all oil classes. Oleic (C18:1) and Palmitoleic (C16:1) were the most abundant in monounsaturated. Palmitic (C16:0) and Stearic (C18:0) were the major saturated fatty acids. Consequently, the breast of P. segnis was a valuable source of high oil quality: It is rich in essential fatty acids and has an important n-3/n-6 ratio in both male and female blue crab. Therefore, the atherogenicity and the thrombogenicity were also evaluated, showing that P. segnis is a shellfish of greater value for human consumption in Tunisia.

The aim of this study is to elucidate the toxicity induced by PbCl

The current study examined the concentrations of ten trace elements (TE) (nickel, chromium, cadmium, iron, zinc, manganese, aluminum, copper, selenium and lead) in the edible tissue of the Ark shell Arca noae (L. 1758) from a Mediterranean coastal lagoon, the Bizerte lagoon during 2013–2014. The analysis of several redox status biomarkers, metallothioneins (MTs), malondialdehyde (MDA), glutathione peroxidase (GPx), reduced glutathione (GSH) and acetylcholinesterase (AChE), was monitored as a response to TE bioaccumulation and environmental parameters variability. Significant differences (p

The flesh of the Pinctada radiata pearl oyster from coastal Tunisia is considered as a high source of n-3 and n-6 and its shell nacre layer is a promising osteogenic biomaterial. Fatty acid (FA) analysis showed that the major components found in total FA (TFA) were 14:0, 16:0, and 18:0 saturated FA (SFA); 16:1, 18:1, and 20:1 monoenoic FA; 20:4n-6 (ARA), 22:5n-3 (DPA). Characteristically high levels of 20:5n-3 (EPA) and 22:6n-3 (DHA) (6.53-89.75 mg/100 g TFA) polyunsaturated FA (PUFA) were found, respectively, in the TFA of nacre and flesh. Evaluated the effects in vitro of lipids extracted from nacre (Ln) and from flesh (Lc) of P. radiata on growth and the differentiation of osteoblasts. Cytotoxicity tests (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide [MTT] and lactic acid dehydrogenase c [LDH]) demonstrated that both extracts are nontoxic. Alizarin Red staining was used in an osteoblast differentiation model using the osteoblast MC3T3-E1 cell line. It showed that the FA of both extracts induced osteoblast differentiation leading to mineralization. Reverse transcription-polymerase chain reaction (RT-PCR) showed a significantly higher expression of osteocalcin (Bglap) and runt-related transcription (Runx2) in MC3T3-E1 cells in the presence of Ln. No difference of osteopontin (Spp1) and Collagen type I (Col1a1) genes compared to the control was observed. In conclusion, these results supported, obtained from our in vitro experimental model used, the interest/potential of lipids extracted from nacre and P. radiata flesh to stimulate bone formation.

In this study the Polychaeta Marphysa sanguinea, was tested to investigate the impact of metal pollution on the environmental state of a coastal Mediterranean lagoon, Tunis Lagoon (Tunisia). A multi-biomarker approach comprising glutathione-stransferase, cyclooxygenase, lysozyme activity, and lipid class composition of the Polychaeta was employed on a seasonal basis in the present investigation. The multivariate statistical approach (principal component analysis and Pearson correlation) clearly demonstrated different spatial patterns in biomarker values and lipid class concentrations. The phospholipids were the dominant lipid class in M. sanguinea, with the highest value found at the control station. The impact of pollution was most clearly observed on the main storage lipid class, triacylglycerol, which was lowest in the most impacted sites. Our work suggests that M. sanguinea can be used in warmer Mediterranean costal habitats as a sentinel species of contaminated ecosystems.

The aim of the present work was to study the effect of season on phospholipids and triacylglycerols (TAG) of mantle and tentacles of female and male wild Sepia officinalis. The identified phospholipids were phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and phosphatidylinositol (PtdIns), and PtdEtn was the major fraction. Results showed apparent seasonal variation of phospholipid content, particularly with female samples. Fatty acid composition of phospholipid classes showed a differentiation much more in the proportions than in the diversity of fatty acids. Results showed that the major saturated fatty acids were 16:0 and 18:0, the major monounsaturated fatty acids were 18:1 and 20:l, and the major polyunsaturated fatty acids were docosahexaenoic acid (22:6n-3) (DHA) and eicosapentaenoic acid (20:5n-3) (EPA). The results relative to TAG demonstrated significant variations. Principal component analysis confirmed the seasonal and sexual effects. This study could be appropriate for the improvement of consistent monitoring of phospholipid and TAG accumulation in cephalopod, which might be important for both physiological studies and food industries.

Acrylamide (ACR), a ubiquitous agent, has various chemical and industrial applications, and it is found in backed or fried carbohydrate-rich food. It has been related to multiple toxicological effects, and it causes high cytotoxicity through oxidative stress. The present study aimed to investigate the potential effect of ACR toxicity administered at different concentrations (5, 10, and 20 mg/L), during 5 days, in order to evaluate the fatty acid (FA) composition and redox state in the digestive gland of Mactra corallina. The results showed, in ACR-treated clams, a significant increase in malondialdehyde, hydrogen peroxide, protein carbonyl, and metallothionein levels, as well as an alteration of the enzymatic (superoxide dismutase, glutathione peroxidase, and catalase) and non-enzymatic (reduced glutathione and ascorbic acid) antioxidant status. However, acetylcholinesterase activity was inhibited in a concentration-dependent manner. In our experiment, the n-3 (Omega-3) and n-6 (Omega-6) polyunsaturated fatty acid levels were significantly changed in all ACR-treated groups. A decrease in eicosapentaenoic acid (C20:5n-3, EPA) and docosahexaenoic acid (C22:6n-3, DHA) was observed in 10-mg/L and 20-mg/L ACR-treated groups. Nevertheless, arachidonic acid (C20:4n-6, ARA) and its precursor linoleic acid (C18:2n-6, LA) were increased. Besides oxidative stress parameters, FA composition may be an additional tool for assessing ACR contamination.

The objective of the present work is to evaluate the possible toxic effect engendered by graded doses of lead chloride (PbCl 2 ) on Mactra stultorum mantle and adductor muscles through a battery of biomarkers responses. M. stultorum were divided into 4 groups and exposed to three concentrations of PbCl 2 (D1:1mg/L, D2: 2.5 mg/L and D3: 5 mg/L) with control during five days. Our findings showed decreases of lipid contents in both organs following PbCl 2 exposure, while, proteins declined only in the adductor muscles of the treated M. stultorum. During our experiment, the PbCl 2 exposure induced the levels of metallothionein (MTs), malondialdehyde (MDA) and advanced oxidation protein products (AOPP) in both organs as compared to the control. These biomarkers responses are distinctly different between mantle and adductor muscles.

Mercury chloride (HgCl2) is a highly toxic compound which provokes a wide range of complicated disorders. The present work aimed to investigate the potential effects of HgCl2 toxicity on the redox state, fatty acids composition and histomorphological aspect of Cerastoderma edule (C. edule) gills and digestive gland. To this end, C. edule were exposed to HgCl2 graded concentrations (1, 10 and 100 µg.L−1) for 6 days. Results showed that HgCl2 exposure caused a significant increase in the levels of hydrogen peroxidase (H2O2) and malondialdehyde (MDA). Fatty acids (FA) analysis in the treated gills and digestive glands indicated an increase in saturated and monounsaturated fatty acids and a decrease in polyunsaturated fatty acids, mainly eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. However, the levels of arachidonic (ARA) and linoleic (C18:2n-6) acids were enhanced especially at the sharpest concentration (100 µg/L). In our investigation, we noticed a significant alteration in the estimated desaturases and elongases’ activities in the treated gills as compared to the digestive glands. A concentration-dependent increase in the enzymatic (superoxide dismutase, glutathione peroxidase and catalase) and non-enzymatic (metallothionein, reduced glutathione and vitamin C) antioxidants was also observed in all treated C. edule. The histopathological changes detected in HgCl2-treated gills and digestive glands varied in a concentration-dependent manner. Overall, this work presented novel evidences about the mechanisms of HgCl2-induced toxicity in bivalves.

Hexavalent chromium (chromium (VI)), a highly toxic heavy metal, is a common pollutant of aquatic ecosystems. The present study aimed to elucidate the potential toxic effects of chromium (VI) on oxidative stress biomarkers and fatty acids profile in the gills and digestive gland of Venus verrucosa, an ecologically and economically important bivalve species. Three doses of chromium (VI) (1, 10 and 100 μg.L−1) were chosen for V. verrucosa exposure during 7 days under controlled conditions. A significant increase in the levels of malondialdehyde, lipid hydroperoxides and hydrogen peroxide was observed in the gills and digestive gland of chromium (VI)-exposed V. verrucosa as compared to the control group. Furthermore, an induction of enzymatic antioxidant activities (superoxide dismutase, glutathione peroxidase and glutathione S-transferase) and an enhancement of non-enzymatic antioxidant levels (non-protein thiols, glutathione and vitamin C) were marked. An alteration of fatty acids composition was also noted following chromium (VI) exposure. The obtained results highlighted the importance of assessing oxidative damage biomarkers and fatty acids profile in the study of chromium (VI)-induced toxicity in V. verrucosa.

Lambda-cyhalothrin (λ-cyh), a synthetic pyrethroid type II, is one of the most toxic xenobiotics to aquatic organisms, which leads to a number of complex toxicological syndromes. The present study aimed to assess the adverse effects of λ-cyh, on fatty acid (FA) composition, redox status, and the histopathology aspect of Venus verrucosa digestive gland under an exposure to a series of concentrations (100, 250 and 500 µg/L). An increase of saturated fatty acid (SFAs) levels and a decrease of polyunsaturated fatty acids (PUFAs) content, mainly eicosapentaenoic (C20: 5n − 3, EPA) and docosahexaenoic (C22: 6n − 3, DHA) acids, were observed in λ-cyh-treated digestive gland. Moreover, our data indicated an alteration in arachidonic acid (C20:4n − 6, ARA) level. The exposure to the highest dose of λ-cyh promoted oxidative stress with an increase of malondialdehyde (MDA), advanced oxidation protein product (AOPP), metallothionein (MT) and hydrogen peroxide (H2O2) levels. A significant decrease in enzymatic (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) and non-enzymatic (reduced glutathione (GSH), vitamin C and non-protein-SH (NPSH)) antioxidants was showed in treated groups. Moreover, acetylcholinesterase (AChE) activity was inhibited with the increase in λ-cyh concentration. Our biochemicals results have been confirmed by the histopathological observations. Overall, our results indicated the toxic effect of λ-cyh on the redox status of V. verrucosa digestive gland and highlighted the usefulness of determining FA composition in the better understanding of λ-cyh toxicity.

The goal of this study was to evaluate the chemical composition and the fatty acid profile of mantle and tentacle of male and female Sepia officinalis, sampled at four seasons from the Mediterranean sea of Tunisia. S. officinalis were found to be rich in glycogen, protein and oil, and significant differences were observed between samples. The level of saturated fatty acid and unsaturated fatty acid showed significant variability among sex and during seasons. DHA and EPA, as polyunsaturated fatty acids, were the most abundant in all samples (14.8–27.8 % and 10.4–18.3 %, respectively). Oleic acid was the most abundant monounsaturated fatty acids (1.63–4.52 %). Σn3 and Σn6 was remarkably different between seasons and among sex. This study could be suitable for the development of reliable guide of fatty acid accumulation in cephalopod.

The effects of artisanal and controlled oven drying methods on the biochemical composition of the body wall of the sea cucumber, Holothuria forskali were investigated. Five combinations of temperature (°C) and relative humidity (%) levels were tested (50°C, 20%, 60°C, 25%; 60°C, 15%, 60°C, 20% and 70°C, 20%). The results demonstrate that the treatment (60°C and 20%) yields an improvement of the nutritional quality of the initial product. Improvements were particularly noted in terms of the amount of total proteins and the polyunsaturated fatty acids level in the dry body wall of H. forskali.

The effects of solar and experimental drying on the protein concentration and total fatty acid (TFA) content of the muscles of Atherina boyeri (sand smelt) were studied. The percentage of protein was 18.3% for fresh fish and 14.2% for sun-dried fish, while TFA content was 4.9 g/100g and 0.5 g/100g. After the drying experiment, the percentage of protein was 23% and the fatty acid (FA) content was 2.8 g/100g. The results show that natural or experimental drying favors saturated fatty acids. The n-3 polyunsaturated fatty acids (PUFAs) are less sensitive to heat than the n-6 PUFAs. From a nutritional point of view, it seems that the drying conditions, where parameters are T = 50˚C, moisture = 30% and air speed = 2 m/s, would be the most beneficial for the preservation of sand smelt.

The effects of the starvation trial on the biochemical composition and the fatty acid dynamics in the triacylglycerol fraction of the digestive gland, gonads and adductor muscle of the scallop Flexopecten glaber were assessed. Results show that three weeks of food deprivation induce depletion of carbohydrates and a significant decrease in proteins and lipids. The noteworthy patterns recorded for the various classes of lipids were the increase of the amount of phosphatidylethanolamine against a strong decline of mono-diacylglycerols, triacylglycerol and phosphatidylserine classes in gonads. These results reflect the ability of Flexopecten glaber to remodel endogenous lipid classes in order to avoid the gonads deterioration. In the starved specimens, severe declines of the n-3 polyunsaturated fatty acid group were recorded in the triacylglycerol fraction of digestive gland and adductor muscle against the increase of this group in gonads. These results confirm the role of triacylglycerol as a polyunsaturated fatty acids reservoir and pointed out to their mobilization from storage organs to the developing gonads during the food shortage trial. Examination of fatty acid data revealed that food deprivation lead Flexopecten glaber to invest in saving and accumulation of highly unsaturated fatty acids in gonads. This applies mainly to the arachidonic acid (20:4n-6) and the docosahexaenoic acid (22:6n-3).

The objective of this study was to compare the composition of fatty acids in the garfish ( Belone belone ) white muscle removed from parasitized and non parasitized specimens. The chemical analysis results revealed low contents of total fatty acids for both parasitized and non-parasitized specimens. Their values, less than 2 g/100 g of fresh muscle, allowed the classification of the garfish as a lean fish. High ratios of saturated fatty acids were found in the garfish muscle reaching 58.4% of total fatty acids. These fatty acids were represented mainly by lauric, miristic and palmitic at a level of 50.3%. As a lean fish, garfish contains 16% polyunsaturated fatty acids (n-3). Two major fatty acids are docosahexaenoic and eicosapentaenoic with respective percentages of 9 and 1.17% of total fatty acids. The parasitized garfish showed increase in their fatty acids, mainly in pentadecanoic, pentadecenoic, docosahexaenoic and arachidonic acids and decreases in saturated acids especially lauric, miristic and palmitic. This drop is correlated with a very significant increase in PUFA from 16 to 26% of total fatty acids. In order to obtain 0.5 g/day of EPA + DHA, the amount of garfish required is 641 g of non-parasitized and 436 g parasitized fish. Key words: Garfish, blood-sucking, parasite, parasitized fish, fatty acid analysis, Tunisia.

The seasonal changes in biochemical composition of the edible bivalveArca noaeharvested from a Mediterranean coastal lagoon (Bizerte lagoon, Tunisia) were investigated from October 2013 to September 2014. Potential food sources and nutritional quality indices (NQI) were determined by analyzing the fatty acid profiles of their tissues during an annual reproductive cycle. Results showed thatA. noaehad moisture (73.8–82%) and protein (24.1–58.6% dry weight) as major components, followed by lipid (10.4–28.8% dry weight) and glycogen (4.05–14.6% dry weight).A. noaeaccumulated lipid and glycogen for gonadal development during both maturation periods (late autumn/late spring–summer) to be used during spawning periods (winter/late summer–early autumn). However, proteins were mainly used to support reproductive allocation and played an important role on the energetic maintenance. Lipid and glycogen were found to be significantly related to temperature, salinity and chlorophylla(p 0.05). An increase of condition index (CI) was observed during spring and early summer when both temperature and food supply increased. The total fatty acid (TFA) profile of Ark shells was dominated by polyunsaturated fatty acids (PUFA) (33.8–49.6%), followed by saturated (SFA) (29.1–43.1%) and monounsaturated fatty acids (MUFA) (20.77–28.3%).A. noaesoft tissues were also rich in macro-minerals (Na, Ca and Mg). The analysis of fatty acid trophic markers revealed that the diet ofA. noaeduring the year of study was based on mixture food mainly on phytoplankton followed by zooplankton and other sources (bacteria, seagrass and macroalgae). Furthermore, the nutritional quality indices have revealed thatA. noaeis an excellent source of nutrients and a healthy food for human consumption. These data are useful to the conservation of natural stocks ofA. noaeand the development of sustainable aquaculture production of this shellfish species in the Bizerte lagoon.

Concentrations of polychlorinated biphenyls (PCBs) were determinated in the muscle tissues of mullets ( Liza aurata ) caught from two coastal marine areas in Tunis bay, “Raoued” and “Rades”. Twenty congeners were detected in all the fish samples analyzed, among which were 4 congeners recommended by the European Union for assessing pollution and 4 “dioxin-like” congeners. Residue levels of total PCBs ranged from 45 to 194 ng/g wet wt in the mullet from “Rades” and from 43 to 65 ng/g w wt in specimens from “Raoued”. Hexachlorobiphenyls were predominant in fishes caught from both “Raoued” and “Rades” areas, accounting for 43% and 40%, respectively. The individual PCB profiles were dominated by congeners 153 in mullet from “Raoued” and PCB 200 in fishes from “Rades”. The levels of TEQs of “dioxin-like” congeners were 3.4 pg/g w wt for fishes from “Raoued” and 2.5 pg/g w wt for specimens from “Rades”.

A partial sequence of mitochondrial cytochrome c oxidase subunit I (COI) was used as a genetic marker for a genetic diversity and phylogenetic analysis (DNA barcoding) of two Mactridae species, Mactra corallina and Eastonia rugosa, collected from the Tunisian coast. These Mactridae species could be distinguished by DNA barcoding techniques and they will be considered as monophyletic clades with the Neighbor-Joining (NJ) tree. The genetic structure detected that E. rugosa presents three haplotypes with a high frequency of HER1 (0.89). However, M. corralina shared 14 haplotypes. The haplotypic diversity (H) was equal to 0.205 and 0.954, respectively, for E. rugosa and M. corallina. While the nucleotide diversity (π) was higher for M. corallina (π=0.0818), the mismatch distribution showed a unimodal curve for E. rugosa (a recent sudden demographic expansion) and a multimodal distribution for M. corallina (size stability).

Muscle and gonad fatty acid compositions from the Pectinids: Pecten jacobaeus , Chlamys varia and Flexopecten glaber , from the Tunisian coast, were analysed. The recorded main fatty acid components in both organs for the three species were C16:0, C18:0, C18:1, C20:5 ω 3 and C22:6 ω 3. Meanwhile, high proportions of EPA and DHA were recorded in the muscle of C. varia (10.1–20.4%), P. jacobaeus (7.68–14.9%), whereas the highest rates (8.98–7.78%) were recorded in gonad for F. glaber . Highest ω 3/ ω 6 ratios were recorded in muscle and gonad of C. varia (9.01 and 7.72, respectively). In P. jacobaeus , the muscle and the gonad had similar nutritional values. Muscle of C. varia had a better fatty acid composition than had the gonad, whilst the gonad value was higher than the muscle in the case of F. glaber .

Changes in body mass and water content (WC), lipids, and fatty acid (FA) composition of muscle were determined in European eels (Anguilla anguilla) in the 'yellow' stage (sexually immature). The animals were held in either freshwater (FW) or seawater (SW) and deprived of food for four weeks. During this period, body mass decreased steadily. This decrease was accompanied by reduced muscle WC and total lipid in both SW and FW. 'Yellow' eels appeared to use lipids in muscle to provide metabolic and osmoregulatory energy. However, they maintained relative constancy in the FA profiles, so that total FA, saturated, monounsaturated, and polyunsaturated FA, were similar between salinities during short-term starvation., This study was supported by a financial aid (grant) from the Agencia Española de Cooperación International (AECI)