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629 results on '"Mezey E"'

6. Atto 465 derivative is a nuclear stain with unique excitation and emission spectra for multiplex immunofluorescence histochemistry

Author

Mezey E, Hogden Ct, Jacqueline W. Mays, Costa-da-Silva Ac, and Joshua Dodge

Subjects
In situ, medicine.diagnostic_test, Chemistry, Microscopy, medicine, Biophysics, Multiplex, Immunofluorescence, Fluorescence, Stain, Visible spectrum, Staining
Abstract

The architecture of a biologic response is inextricably linked with the tissue architecture of the target site. Multiplex immunofluorescence (mIF) is an effective technique for the maximal visualization of multiple target proteins in situ. This powerful tool is limited by the spectral overlap separation of the currently available synthetic fluorescent dyes. The fluorescence excitation wavelengths ranging between 405nm and 488nm are rarely used in mIF imaging and serve as a logical additional slot for a fluorescent probe. In the present study, we demonstrate that Atto 465-pentafluoroaniline (Atto 465-p), a derivative of the fluorescent dye Atto 465, can serve as a nuclear stain in the violet-blue region of the visible spectrum. This opens the 405nm channel, traditionally used for nuclear stain, for detection of another experimental target. This increases the flexibility of the mIF panel and, with appropriate staining and microscopy, enables the quantitative analysis of at least six targets in one tissue section.

Published
2021

12. Optical observations on milli-, micro-, and nanosecond timescales

Author

Dravins, D., Lindegren, L., Mezey, E., Araki, H., editor, Brézin, E., editor, Ehlers, J., editor, Frisch, U., editor, Hepp, K., editor, Jaffe, R. L., editor, Kippenhahn, R., editor, Weidenmüller, H. A., editor, Wess, J., editor, Zittartz, J., editor, Beiglböck, W., editor, Greiner, Jochen, editor, Duerbeck, Hilmar W., editor, and Gershberg, Roald E., editor

Published
1995
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13. Preeclampsia is Associated with Sex-Specific Transcriptional and Proteomic Changes in Fetal Erythroid Cells

Author

Masoumi, Z, Maes, GE, Herten, K, Cortes-Calabuig, A, Alattar, AG, Hanson, E, Erlandsson, L, Mezey, E, Magnusson, M, Vermeesch, JR, Familari, M, Hansson, SR, Masoumi, Z, Maes, GE, Herten, K, Cortes-Calabuig, A, Alattar, AG, Hanson, E, Erlandsson, L, Mezey, E, Magnusson, M, Vermeesch, JR, Familari, M, and Hansson, SR

Abstract

Preeclampsia (PE) has been associated with placental dysfunction, resulting in fetal hypoxia, accelerated erythropoiesis, and increased erythroblast count in the umbilical cord blood (UCB). Although the detailed effects remain unknown, placental dysfunction can also cause inflammation, nutritional, and oxidative stress in the fetus that can affect erythropoiesis. Here, we compared the expression of surface adhesion molecules and the erythroid differentiation capacity of UCB hematopoietic stem/progenitor cells (HSPCs), UCB erythroid profiles along with the transcriptome and proteome of these cells between male and female fetuses from PE and normotensive pregnancies. While no significant differences were observed in UCB HSPC migration/homing and in vitro erythroid colony differentiation, the UCB HSPC transcriptome and the proteomic profile of the in vitro differentiated erythroid cells differed between PE vs. normotensive samples. Accordingly, despite the absence of significant differences in the UCB erythroid populations in male or female fetuses from PE or normotensive pregnancies, transcriptional changes were observed during erythropoiesis, particularly affecting male fetuses. Pathway analysis suggested deregulation in the mammalian target of rapamycin complex 1/AMP-activated protein kinase (mTORC1/AMPK) signaling pathways controlling cell cycle, differentiation, and protein synthesis. These results associate PE with transcriptional and proteomic changes in fetal HSPCs and erythroid cells that may underlie the higher erythroblast count in the UCB in PE.

Published
2019

24. Mice lacking D5 dopamine receptors have increased sympathetic tone and are hypertensive.

Author

Jose P.A., Sibley D.R., Hollon T.R., Bek M.J., Lachowicz J.E., Ariano M.A., Mezey E., Ramachandran R., Wersinger S.R., Soares-da-Silva P., Liu Z.F., Grinberg A., Drago J., Young III W.S., Westphal H., Jose P.A., Sibley D.R., Hollon T.R., Bek M.J., Lachowicz J.E., Ariano M.A., Mezey E., Ramachandran R., Wersinger S.R., Soares-da-Silva P., Liu Z.F., Grinberg A., Drago J., Young III W.S., and Westphal H.

Abstract

Dopamine is an important transmitter in the CNS and PNS, critically regulating numerous neuropsychiatric and physiological functions. These actions of dopamine are mediated by five distinct receptor subtypes. Of these receptors, probably the least understood in terms of physiological functions is the D5 receptor subtype. To better understand the role of the D5 dopamine receptor (DAR) in normal physiology and behavior, we have now used gene-targeting technology to create mice that lack this receptor subtype. We find that the D5 receptor-deficient mice are viable and fertile and appear to develop normally. No compensatory alterations in other dopamine receptor subtypes were observed. We find, however, that the mutant mice develop hypertension and exhibit significantly elevated blood pressure (BP) by 3 months of age. This hypertension appears to be caused by increased sympathetic tone, primarily attributable to a CNS defect. Our data further suggest that this defect involves an oxytocin-dependent sensitization of V1 vasopressin and non-NMDA glutamatergic receptor-mediated pathways, potentially within the medulla, leading to increased sympathetic outflow. These results indicate that D5 dopamine receptors modulate neuronal pathways regulating blood pressure responses and may provide new insights into mechanisms for some forms of essential hypertension in humans, a disease that afflicts up to 25% of the aged adult population in industrialized societies.

Published
2003

32. B-adrenergic mechanism of insulin-induced adrenocorticotropin release from the anterior pituitary

Author

Mezey, E., Reisine, T.D., Brownstein, M.J., Palkovits, M., and Axelrod, J.

Subjects
Insulin -- Physiological aspects -- Research, ACTH -- Physiological aspects -- Research, Science and technology, Physiological aspects, Research
Abstract

Insulin is vital in maintaining the normal metabolic state of the body. It is released from the pancreas and reduces circulating glucose by enhancing the uptake of glucose into cells. [...]

Published
1984

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