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11 results on '"Meulenbroeks, Chantal"'

5. Basophil-Derived Amphiregulin Is Essential for UVB Irradiation-Induced Immune Suppression

Author

Meulenbroeks, Chantal, van Weelden, Huib, Schwartz, Christian, Voehringer, David, Redegeld, Frank A M, Rutten, Victor P M G, Willemse, Ton, Sijts, Alice J A M, Zaiss, Dietmar M W, Sub Immunopharmacology, LS Immunologie, Beheer, Pharmacology, CSCA AVM, Advances in Veterinary Medicine, I&I RATIA-SIB, and dI&I RA-I&I I&I

Abstract

UVB irradiation (290-320 nm) is used to treat skin diseases like psoriasis and atopic dermatitis, and is known to suppress contact hypersensitivity (CHS) reactions in mouse models. Regulatory T cells (Treg cells) have been shown to be responsible for this UVB-induced suppression of CHS. The epidermal growth factor (EGF)-like growth factor amphiregulin (AREG) engages EGFR on Treg cells and, in different disease models, it was shown that mast cell-derived AREG is essential for optimal Treg cell function in vivo. Here we determined whether AREG has a role in UVB-induced, Treg cell-mediated suppression of CHS reactions in the skin. Our data show that AREG is essential for UVB-induced CHS suppression. In contrast to the general assumption, however, mast cells were dispensable for UVB-induced immune suppression, whereas basophil-derived AREG was essential. These data reveal, to our knowledge, a previously unreported function for basophils in the homeostasis of immune responses in the skin. Basophils thus fulfill a dual function: they contribute to the initiation of effective type 2 immune responses and, by enhancing the suppressive capacity of local Treg cell populations, also to local immune regulation in the skin.Journal of Investigative Dermatology advance online publication, 11 September 2014; (2014) 0, 000-000. doi:10.1038/jid.2014.329.

Published
2015

10. Enhanced Inflammatory Potential of CD4+ T-Cells That Lack Proteasome Immunosubunit Expression, in a T-Cell Transfer-Based Colitis Model.

Author

Rasid, Orhan, Meulenbroeks, Chantal, Gröne, Andrea, Zaiss, Dietmar, and Sijts, Alice

Subjects
*BIODEGRADATION, *INFLAMMATION, *CD4 antigen, *T cells, *PROTEASOMES, *COLITIS, *CYTOKINES, *IMMUNOLOGY
Abstract

Proteasomes play a fundamental role in intracellular protein degradation and therewith regulate a variety of cellular processes. Exposure of cells to (pro)inflammatory cytokines upregulates the expression of three inducible catalytic proteasome subunits, the immunosubunits, which incorporate into newly assembled proteasome complexes and alter the catalytic activity of the cellular proteasome population. Single gene-deficient mice lacking one of the three immunosubunits are resistant to dextran sulfate sodium (DSS)-induced colitis development and, likewise, inhibition of one single immunosubunit protects mice against the development of DSS-induced colitis. The observed diminished disease susceptibility has been attributed to altered cytokine production and CD4+ T-cell differentiation in the absence of immunosubunits. To further test whether the catalytic activity conferred by immunosubunits plays an essential role in CD4+ T-cell function and to distinguish between the role of immunosubunits in effector T-cells versus inflamed tissue, we used a T-cell transfer-induced colitis model. Naïve wt or immunosubunit-deficient CD4+ T-cells were adoptively transferred into RAG1−/− and immunosubunit-deficient RAG1−/− mice and colitis development was determined six weeks later. While immunosubunit expression in recipient mice had no effect on colitis development, transferred immunosubunit-deficient T- cells were more potent in inducing colitis and produced more proinflammatory IL17 than wt T-cells. Taken together, our data show that modifications in proteasome-mediated proteolysis in T-cells, conferred by lack of immunosubunit incorporation, do not attenuate but enhance CD4+ T-cell-induced inflammation. [ABSTRACT FROM AUTHOR]

Published
2014
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11. Basophil-Derived Amphiregulin Is Essential for UVB Irradiation–Induced Immune Suppression

Author

Meulenbroeks, Chantal, van Weelden, Huib, Schwartz, Christian, Voehringer, David, Redegeld, Frank A M, Rutten, Victor P M G, Willemse, Ton, Sijts, Alice J A M, Zaiss, Dietmar M W, Sub Immunopharmacology, LS Immunologie, Beheer, Pharmacology, CSCA AVM, Advances in Veterinary Medicine, I&I RATIA-SIB, and dI&I RA-I&I I&I

Subjects
Male, EGF Family of Proteins, medicine.medical_treatment, chemical and pharmacologic phenomena, Dermatology, Basophil, Biology, Dermatitis, Contact, Amphiregulin, T-Lymphocytes, Regulatory, Ultraviolet therapy, Biochemistry, Immune system, Epidermal growth factor, Psoriasis, Immune Tolerance, medicine, Animals, Mast Cells, skin and connective tissue diseases, Molecular Biology, Mice, Knockout, integumentary system, Growth factor, hemic and immune systems, Cell Biology, medicine.disease, Basophils, Mice, Inbred C57BL, Disease Models, Animal, medicine.anatomical_structure, Immunology, Female, Ultraviolet Therapy, Bone marrow
Abstract

UVB irradiation (290-320 nm) is used to treat skin diseases like psoriasis and atopic dermatitis, and is known to suppress contact hypersensitivity (CHS) reactions in mouse models. Regulatory T cells (Treg cells) have been shown to be responsible for this UVB-induced suppression of CHS. The epidermal growth factor (EGF)-like growth factor amphiregulin (AREG) engages EGFR on Treg cells and, in different disease models, it was shown that mast cell-derived AREG is essential for optimal Treg cell function in vivo. Here we determined whether AREG has a role in UVB-induced, Treg cell-mediated suppression of CHS reactions in the skin. Our data show that AREG is essential for UVB-induced CHS suppression. In contrast to the general assumption, however, mast cells were dispensable for UVB-induced immune suppression, whereas basophil-derived AREG was essential. These data reveal, to our knowledge, a previously unreported function for basophils in the homeostasis of immune responses in the skin. Basophils thus fulfill a dual function: they contribute to the initiation of effective type 2 immune responses and, by enhancing the suppressive capacity of local Treg cell populations, also to local immune regulation in the skin.Journal of Investigative Dermatology advance online publication, 11 September 2014; (2014) 0, 000-000. doi:10.1038/jid.2014.329.

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