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950 results on '"Mestroni, Luisa"'

2. Abstract 4147845: Valsartan and Cardiac Remodeling in Early-Stage Hypertrophic Cardiomyopathy: The VANISH Trial Cardiac Magnetic Resonance Substudy

Author

Ostrominski, John, Claggett, Brian, Jerosch-Herold, Michael, Axelsson, Anna, Day, Sharlene, Russell, Mark, Zahka, Kenneth, Pereira, Alexandre, Colan, Steven, Murphy, Anne, Canter, Charles, Bach, Richard, Wheeler, Matthew, Rossano, Joseph, Owens, Anjali, Mestroni, Luisa, Taylor, Matthew, Patel, Amit, Wilmot, Ivan, Soslow, Jonathan, Becker, Jason, Lakdawala, Neal, Bundgaard, Henning, Vargas, Jose, and Ho, Carolyn

Published
2024
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3. Abstract 4142400: Clinical implication and potential role of immunosuppression for myocarditis episodes in patients with desmoplakin cardiomyopathy: hope from the DSP-ERADOS Network?

Author

Gasperetti, Alessio, Peretto, Giovanni, Muller, Steven, Laredo, mikael, Carrick, Richard, Asatryan, Babken, Protonotarios, Alexandros, Murray, Brittney, Scheel, Paul, Pilichou, Kalliopi, Syrris, Petros, Jason, Max, Medo, Kristen, Rossi, Valentina, Saguner, Ardan, Hylind, Robyn, Abrams, Dominic, Cadrin-Tourigny, Julia, olivotto, iacopo, Biagini, Elena, Cox, Moniek, CHARRON, Philippe, Ware, James, Cal, Leonardo, smith, eric, Ingles, Jodie, Ader, Flavie, Peters, Stacey, russo, jeremy, Zentner, Dominica, Bahr, Eric, Carruth, Eric, Haggerty, Chris, Parikh, Victoria, Taylor, Matthew, Mestroni, Luisa, Sinagra, Gianfranco, Wilde, Arthur, Gandjbakhch, Estelle, Te Riele, Anneline, Van Tintelen, Peter, Elliott, Perry, Adamo, Luigi, James, Cynthia, Merlo, Marco, Lakdawala, Neal, Bauce, Barbara, Helms, Adam, Calkins, Hugh, and Gilotra, Nisha

Published
2024
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6. Abstract 4137272: The Impact of Genotype in Dilated and Arrhythmogenic Cardiomyopathy: Insights from the SHaRE Registry

Author

Padalia, Kishan, Slaven, Sarah, Medo, Kristen, Graw, Sharon, Claggett, Brian, Bundgaard, Henning, Helms, Adam, Heymans, Stephane, Ho, Carolyn, Khan, Sadiya, Lakdawala, Neal, Olivotto, Iacopo, Owens, Anjali, Prasad, Sanjay, Axelsson, Anna, Reza, Nosheen, Shore, Supriya, sinagra, Gianfranco, Tayal, Upasana, Verdonschot, Job, Wheeler, Matthew, Taylor, Matthew, Merlo, Marco, Fornaro, Alessandra, Parikh, Victoria, Wilsbacher, Lisa, and Mestroni, Luisa

Published
2024
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7. A Systematic Analysis of the Clinical Outcome Associated with Multiple Reclassified Desmosomal Gene Variants in Arrhythmogenic Right Ventricular Cardiomyopathy Patients

Author

Nagyova, Emilia, Hoorntje, Edgar T., te Rijdt, Wouter P., Bosman, Laurens P., Syrris, Petros, Protonotarios, Alexandros, Elliott, Perry M., Tsatsopoulou, Adalena, Mestroni, Luisa, Taylor, Matthew R. G., Sinagra, Gianfranco, Merlo, Marco, Wada, Yuko, Horie, Minoru, Mogensen, Jens, Christensen, Alex H., Gerull, Brenda, Song, Lei, Yao, Yan, Fan, Siyang, Saguner, Ardan M., Duru, Firat, Koskenvuo, Juha W., Cruz Marino, Tania, Tichnell, Crystal, Judge, Daniel P., Dooijes, Dennis, Lekanne Deprez, Ronald H., Basso, Cristina, Pilichou, Kalliopi, Bauce, Barbara, Wilde, Arthur A. M., Charron, Philippe, Fressart, Véronique, van der Heijden, Jeroen F., van den Berg, Maarten P., Asselbergs, Folkert W., James, Cynthia A., Jongbloed, Jan D. H., Harakalova, Magdalena, and van Tintelen, J. Peter

Published
2023
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11. A genetic association study of circulating coagulation factor VIII and von Willebrand factor levels

Author

Abe, Namiko, Abecasis, Gonçalo, Aguet, Francois, Albert, Christine, Almasy, Laura, Alonso, Alvaro, Ament, Seth, Anderson, Peter, Anugu, Pramod, Applebaum-Bowden, Deborah, Ardlie, Kristin, Arking, Dan, Arnett, Donna K, Ashley-Koch, Allison, Aslibekyan, Stella, Assimes, Tim, Auer, Paul, Avramopoulos, Dimitrios, Ayas, Najib, Balasubramanian, Adithya, Barnard, John, Barnes, Kathleen, Barr, R. Graham, Barron-Casella, Emily, Barwick, Lucas, Beaty, Terri, Beck, Gerald, Becker, Diane, Becker, Lewis, Beer, Rebecca, Beitelshees, Amber, Benjamin, Emelia, Benos, Takis, Bezerra, Marcos, Bielak, Larry, Bis, Joshua, Blackwell, Thomas, Blangero, John, Blue, Nathan, Boerwinkle, Eric, Bowden, Donald W., Bowler, Russell, Brody, Jennifer, Broeckel, Ulrich, Broome, Jai, Brown, Deborah, Bunting, Karen, Burchard, Esteban, Bustamante, Carlos, Buth, Erin, Cade, Brian, Cardwell, Jonathan, Carey, Vincent, Carrier, Julie, Carson, April P., Carty, Cara, Casaburi, Richard, Casas Romero, Juan P, Casella, James, Castaldi, Peter, Chaffin, Mark, Chang, Christy, Chang, Yi-Cheng, Chasman, Daniel, Chavan, Sameer, Chen, Bo-Juen, Chen, Wei-Min, Ida Chen, Yii-Der, Cho, Michael, Choi, Seung Hoan, Chuang, Lee-Ming, Chung, Mina, Chung, Ren-Hua, Clish, Clary, Comhair, Suzy, Conomos, Matthew, Cornell, Elaine, Correa, Adolfo, Crandall, Carolyn, Crapo, James, Cupples, L. Adrienne, Curran, Joanne, Curtis, Jeffrey, Custer, Brian, Damcott, Coleen, Darbar, Dawood, David, Sean, Davis, Colleen, Daya, Michelle, de Andrade, Mariza, de las Fuentes, Lisa, de Vries, Paul, DeBaun, Michael, Deka, Ranjan, DeMeo, Dawn, Devine, Scott, Dinh, Huyen, Doddapaneni, Harsha, Duan, Qing, Dugan-Perez, Shannon, Duggirala, Ravi, Durda, Jon Peter, Dutcher, Susan K., Eaton, Charles, Ekunwe, Lynette, El Boueiz, Adel, Ellinor, Patrick, Emery, Leslie, Erzurum, Serpil, Farber, Charles, Farek, Jesse, Fingerlin, Tasha, Flickinger, Matthew, Fornage, Myriam, Franceschini, Nora, Frazar, Chris, Fu, Mao, Fullerton, Stephanie M., Fulton, Lucinda, Gabriel, Stacey, Gan, Weiniu, Gao, Shanshan, Gao, Yan, Gass, Margery, Geiger, Heather, Gelb, Bruce, Geraci, Mark, Germer, Soren, Gerszten, Robert, Ghosh, Auyon, Gibbs, Richard, Gignoux, Chris, Gladwin, Mark, Glahn, David, Gogarten, Stephanie, Gong, Da-Wei, Goring, Harald, Graw, Sharon, Gray, Kathryn J., Grine, Daniel, Gross, Colin, Gu, C. Charles, Guan, Yue, Guo, Xiuqing, Gupta, Namrata, Haessler, Jeff, Hall, Michael, Han, Yi, Hanly, Patrick, Harris, Daniel, Hawley, Nicola L., He, Jiang, Heavner, Ben, Heckbert, Susan, Hernandez, Ryan, Herrington, David, Hersh, Craig, Hidalgo, Bertha, Hixson, James, Hobbs, Brian, Hokanson, John, Hong, Elliott, Hoth, Karin, Hsiung, Chao (Agnes), Hu, Jianhong, Hung, Yi-Jen, Huston, Haley, Hwu, Chii Min, Irvin, Marguerite Ryan, Jackson, Rebecca, Jain, Deepti, Jaquish, Cashell, Johnsen, Jill, Johnson, Andrew, Johnson, Craig, Johnston, Rich, Jones, Kimberly, Kang, Hyun Min, Kaplan, Robert, Kardia, Sharon, Kelly, Shannon, Kenny, Eimear, Kessler, Michael, Khan, Alyna, Khan, Ziad, Kim, Wonji, Kimoff, John, Kinney, Greg, Konkle, Barbara, Kooperberg, Charles, Kramer, Holly, Lange, Christoph, Lange, Ethan, Lange, Leslie, Laurie, Cathy, Laurie, Cecelia, LeBoff, Meryl, Lee, Jiwon, Lee, Sandra, Lee, Wen-Jane, LeFaive, Jonathon, Levine, David, Levy, Dan, Lewis, Joshua, Li, Xiaohui, Li, Yun, Lin, Henry, Lin, Honghuang, Lin, Xihong, Liu, Simin, Liu, Yongmei, Liu, Yu, Loos, Ruth J. F., Lubitz, Steven, Lunetta, Kathryn, Luo, James, Magalang, Ulysses, Mahaney, Michael, Make, Barry, Manichaikul, Ani, Manning, Alisa, Manson, JoAnn, Martin, Lisa, Marton, Melissa, Mathai, Susan, Mathias, Rasika, May, Susanne, McArdle, Patrick, McDonald, Merry-Lynn, McFarland, Sean, McGarvey, Stephen, McGoldrick, Daniel, McHugh, Caitlin, McNeil, Becky, Mei, Hao, Meigs, James, Menon, Vipin, Mestroni, Luisa, Metcalf, Ginger, Meyers, Deborah A, Mignot, Emmanuel, Mikulla, Julie, Min, Nancy, Minear, Mollie, Minster, Ryan L, Mitchell, Braxton D., Moll, Matt, Momin, Zeineen, Montasser, May E., Montgomery, Courtney, Muzny, Donna, Mychaleckyj, Josyf C, Nadkarni, Girish, Naik, Rakhi, Naseri, Take, Natarajan, Pradeep, Nekhai, Sergei, Nelson, Sarah C., Neltner, Bonnie, Nessner, Caitlin, Nickerson, Deborah, Nkechinyere, Osuji, North, Kari, O'Connell, Jeff, O'Connor, Tim, Ochs-Balcom, Heather, Okwuonu, Geoffrey, Pack, Allan, Paik, David T., Palmer, Nicholette, Pankow, James, Papanicolaou, George, Parker, Cora, Peloso, Gina, Peralta, Juan Manuel, Perez, Marco, Perry, James, Peters, Ulrike, Peyser, Patricia, Phillips, Lawrence S, Pleiness, Jacob, Pollin, Toni, Post, Wendy, Becker, Julia Powers, Boorgula, Meher Preethi, Preuss, Michael, Psaty, Bruce, Qasba, Pankaj, Qiao, Dandi, Qin, Zhaohui, Rafaels, Nicholas, Raffield, Laura, Rajendran, Mahitha, Ramachandran, Vasan S., Rao, D. C., Rasmussen-Torvik, Laura, Ratan, Aakrosh, Redline, Susan, Reed, Robert, Reeves, Catherine, Regan, Elizabeth, Reiner, Alex, Reupena, Muagututi‘a Sefuiva, Rice, Ken, Rich, Stephen, Robillard, Rebecca, Robine, Nicolas, Roden, Dan, Roselli, Carolina, Rotter, Jerome, Ruczinski, Ingo, Runnels, Alexi, Russell, Pamela, Ruuska, Sarah, Ryan, Kathleen, Sabino, Ester Cerdeira, Saleheen, Danish, Salimi, Shabnam, Salvi, Sejal, Salzberg, Steven, Sandow, Kevin, Sankaran, Vijay G., Santibanez, Jireh, Schwander, Karen, Schwartz, David, Sciurba, Frank, Seidman, Christine, Seidman, Jonathan, Sériès, Frédéric, Sheehan, Vivien, Sherman, Stephanie L., Shetty, Amol, Shetty, Aniket, Hui-Heng Sheu, Wayne, Shoemaker, M. Benjamin, Silver, Brian, Silverman, Edwin, Skomro, Robert, Smith, Albert Vernon, Smith, Jennifer, Smith, Josh, Smith, Nicholas, Smith, Tanja, Smoller, Sylvia, Snively, Beverly, Snyder, Michael, Sofer, Tamar, Sotoodehnia, Nona, Stilp, Adrienne M., Storm, Garrett, Streeten, Elizabeth, Su, Jessica Lasky, Sung, Yun Ju, Sylvia, Jody, Szpiro, Adam, Taliun, Daniel, Tang, Hua, Taub, Margaret, Taylor, Kent D., Taylor, Matthew, Taylor, Simeon, Telen, Marilyn, Thornton, Timothy A., Threlkeld, Machiko, Tinker, Lesley, Tirschwell, David, Tishkoff, Sarah, Tiwari, Hemant, Tong, Catherine, Tracy, Russell, Tsai, Michael, Vaidya, Dhananjay, Van Den Berg, David, VandeHaar, Peter, Vrieze, Scott, Walker, Tarik, Wallace, Robert, Walts, Avram, Wang, Fei Fei, Wang, Heming, Wang, Jiongming, Watson, Karol, Watt, Jennifer, Weeks, Daniel E., Weinstock, Joshua, Weir, Bruce, Weiss, Scott T, Weng, Lu-Chen, Wessel, Jennifer, Willer, Cristen, Williams, Kayleen, Williams, L. Keoki, Wilson, Carla, Wilson, James, Winterkorn, Lara, Wong, Quenna, Wu, Joseph, Xu, Huichun, Yanek, Lisa, Yang, Ivana, Yu, Ketian, Zekavat, Seyedeh Maryam, Zhang, Yingze, Zhao, Snow Xueyan, Zhao, Wei, Zhu, Xiaofeng, Ziv, Elad, Zody, Michael, Zoellner, Sebastian, Lindstrom, Sara, Wang, Lu, Smith, Erin N., Gordon, William, van Hylckama Vlieg, Astrid, Brody, Jennifer A., Pattee, Jack W., Haessler, Jeffrey, Brumpton, Ben M., Chasman, Daniel I., Suchon, Pierre, Chen, Ming-Huei, Turman, Constance, Germain, Marine, Wiggins, Kerri L., MacDonald, James, Braekkan, Sigrid K., Armasu, Sebastian M., Pankratz, Nathan, Jackson, Rabecca D., Nielsen, Jonas B., Giulianini, Franco, Puurunen, Marja K., Ibrahim, Manal, Heckbert, Susan R., Bammler, Theo K., Frazer, Kelly A., McCauley, Bryan M., Taylor, Kent, Pankow, James S., Reiner, Alexander P., Gabrielsen, Maiken E., Deleuze, Jean-François, O'Donnell, Chris J., Kim, Jihye, McKnight, Barbara, Kraft, Peter, Hansen, John-Bjarne, Rosendaal, Frits R., Heit, John A., Psaty, Bruce M., Tang, Weihong, Hveem, Kristian, Ridker, Paul M., Morange, Pierre-Emmanuel, Johnson, Andrew D., Kabrhel, Christopher, AlexandreTrégouët, David, Smith, Nicholas L., de Vries, Paul S., Reventun, Paula, Brown, Michael R., Heath, Adam S., Huffman, Jennifer E., Le, Ngoc-Quynh, Bebo, Allison, Temprano-Sagrera, Gerard, Raffield, Laura M., Ozel, Ayse Bilge, Thibord, Florian, Lewis, Joshua P., Rodriguez, Benjamin A. T., Polasek, Ozren, Yanek, Lisa R., Carrasquilla, German D., Marioni, Riccardo E., Kleber, Marcus E., Trégouët, David-Alexandre, Yao, Jie, Li-Gao, Ruifang, Joshi, Peter K., Trompet, Stella, Martinez-Perez, Angel, Ghanbari, Mohsen, Howard, Tom E., Reiner, Alex P., Arvanitis, Marios, Ryan, Kathleen A., Bartz, Traci M., Rudan, Igor, Faraday, Nauder, Linneberg, Allan, Davies, Gail, Delgado, Graciela E., Klaric, Lucija, Noordam, Raymond, van Rooij, Frank, Curran, Joanne E., Wheeler, Marsha M., Osburn, William O., O'Connell, Jeffrey R., Beswick, Andrew, Kolcic, Ivana, Souto, Juan Carlos, Becker, Lewis C., Hansen, Torben, Doyle, Margaret F., Harris, Sarah E., Moissl, Angela P., Rich, Stephen S., Campbell, Harry, Stott, David J., Soria, Jose Manuel, de Maat, Moniek P. M., Brody, Lawrence C., Auer, Paul L., Ben-Shlomo, Yoav, Hayward, Caroline, Mathias, Rasika A., Kilpeläinen, Tuomas O., Lange, Leslie A., Cox, Simon R., März, Winfried, Rotter, Jerome I., Mook-Kanamori, Dennis O., Wilson, James F., van der Harst, Pim, Jukema, J. Wouter, Ikram, M. Arfan, Desch, Karl C., Sabater-Lleal, Maria, Lowenstein, Charles J., and Morrison, Alanna C.

Published
2024
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12. Long-Term Arrhythmic Follow-Up and Risk Stratification of Patients With Desmoplakin-Associated Arrhythmogenic Right Ventricular Cardiomyopathy

Author

Gasperetti, Alessio, Carrick, Richard, Protonotarios, Alexandros, Laredo, Mikael, van der Schaaf, Iris, Syrris, Petros, Murray, Brittney, Tichnell, Crystal, Cappelletto, Chiara, Gigli, Marta, Medo, Kristen, Crabtree, Peter, Saguner, Ardan M., Duru, Firat, Hylind, Robyn, Abrams, Dominic, Lakdawala, Neal K., Massie, Charles, Cadrin-Tourigny, Julia, Targetti, Mattia, Olivotto, Iacopo, Graziosi, Maddalena, Cox, Moniek, Biagini, Elena, Charron, Philippe, Casella, Michela, Tondo, Claudio, Yazdani, Momina, Ware, James S., Prasad, Sanjay, Calò, Leonardo, Smith, Eric, Helms, Adam, Hespe, Sophie, Ingles, Jodie, Tandri, Harikrishna, Ader, Flavie, Mestroni, Luisa, Wilde, Arthur, Merlo, Marco, Gandjbakhch, Estelle, Calkins, Hugh, te Riele, Anneline S.J.M., Peter van Tintelen, J., Elliot, Perry, and James, Cynthia A.

Published
2024
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14. Epigenome-wide DNA methylation association study of circulating IgE levels identifies novel targets for asthma

Author

Abe, Namiko, Abecasis, Gonçalo, Aguet, Francois, Albert, Christine, Almasy, Laura, Alonso, Alvaro, Ament, Seth, Anderson, Peter, Anugu, Pramod, Applebaum-Bowden, Deborah, Ardlie, Kristin, Arking, Dan, Arnett, Donna K., Ashley-Koch, Allison, Aslibekyan, Stella, Assimes, Tim, Auer, Paul, Avramopoulos, Dimitrios, Ayas, Najib, Balasubramanian, Adithya, Barnard, John, Barnes, Kathleen, Barr, R. Graham, Barron-Casella, Emily, Barwick, Lucas, Beaty, Terri, Beck, Gerald, Becker, Diane, Becker, Lewis, Beer, Rebecca, Beitelshees, Amber, Benjamin, Emelia, Benos, Takis, Bezerra, Marcos, Bielak, Larry, Bis, Joshua, Blackwell, Thomas, Blangero, John, Blue, Nathan, Boerwinkle, Eric, Bowden, Donald W., Bowler, Russell, Brody, Jennifer, Broeckel, Ulrich, Broome, Jai, Brown, Deborah, Bunting, Karen, Burchard, Esteban, Bustamante, Carlos, Buth, Erin, Cade, Brian, Cardwell, Jonathan, Carey, Vincent, Carrier, Julie, Carson, April P., Carty, Cara, Casaburi, Richard, Casas Romero, Juan P., Casella, James, Castaldi, Peter, Chaffin, Mark, Chang, Christy, Chang, Yi-Cheng, Chasman, Daniel, Chavan, Sameer, Chen, Bo-Juen, Chen, Wei-Min, Ida Chen, Yii-Der, Cho, Michael, Choi, Seung Hoan, Chuang, Lee-Ming, Chung, Mina, Chung, Ren-Hua, Clish, Clary, Comhair, Suzy, Conomos, Matthew, Cornell, Elaine, Correa, Adolfo, Crandall, Carolyn, Crapo, James, Cupples, L. Adrienne, Curran, Joanne, Curtis, Jeffrey, Custer, Brian, Damcott, Coleen, Darbar, Dawood, David, Sean, Davis, Colleen, Daya, Michelle, de Andrade, Mariza, de las Fuentes, Lisa, de Vries, Paul, DeBaun, Michael, Deka, Ranjan, DeMeo, Dawn, Devine, Scott, Dinh, Huyen, Doddapaneni, Harsha, Duan, Qing, Dugan-Perez, Shannon, Duggirala, Ravi, Durda, Jon Peter, Dutcher, Susan K., Eaton, Charles, Ekunwe, Lynette, El Boueiz, Adel, Ellinor, Patrick, Emery, Leslie, Erzurum, Serpil, Farber, Charles, Farek, Jesse, Fingerlin, Tasha, Flickinger, Matthew, Fornage, Myriam, Franceschini, Nora, Frazar, Chris, Fu, Mao, Fullerton, Stephanie M., Fulton, Lucinda, Gabriel, Stacey, Gan, Weiniu, Gao, Shanshan, Gao, Yan, Gass, Margery, Geiger, Heather, Gelb, Bruce, Geraci, Mark, Germer, Soren, Gerszten, Robert, Ghosh, Auyon, Gibbs, Richard, Gignoux, Chris, Gladwin, Mark, Glahn, David, Gogarten, Stephanie, Gong, Da-Wei, Goring, Harald, Graw, Sharon, Gray, Kathryn J., Grine, Daniel, Gross, Colin, Gu, C. Charles, Guan, Yue, Guo, Xiuqing, Gupta, Namrata, Haessler, Jeff, Hall, Michael, Han, Yi, Hanly, Patrick, Harris, Daniel, Hawley, Nicola L., He, Jiang, Heavner, Ben, Heckbert, Susan, Hernandez, Ryan, Herrington, David, Hersh, Craig, Hidalgo, Bertha, Hixson, James, Hobbs, Brian, Hokanson, John, Hong, Elliott, Hoth, Karin, Hsiung, Chao (Agnes), Hu, Jianhong, Hung, Yi-Jen, Huston, Haley, Hwu, Chii Min, Irvin, Marguerite Ryan, Jackson, Rebecca, Jain, Deepti, Jaquish, Cashell, Johnsen, Jill, Johnson, Andrew, Johnson, Craig, Johnston, Rich, Jones, Kimberly, Kang, Hyun Min, Kaplan, Robert, Kardia, Sharon, Kelly, Shannon, Kenny, Eimear, Kessler, Michael, Khan, Alyna, Khan, Ziad, Kim, Wonji, Kimoff, John, Kinney, Greg, Konkle, Barbara, Kooperberg, Charles, Kramer, Holly, Lange, Christoph, Lange, Ethan, Lange, Leslie, Laurie, Cathy, Laurie, Cecelia, LeBoff, Meryl, Lee, Jiwon, Lee, Sandra, Lee, Wen-Jane, LeFaive, Jonathon, Levine, David, Levy, Daniel, Lewis, Joshua, Li, Xiaohui, Li, Yun, Lin, Henry, Lin, Honghuang, Lin, Xihong, Liu, Simin, Liu, Yongmei, Liu, Yu, Loos, Ruth J.F., Lubitz, Steven, Lunetta, Kathryn, Luo, James, Magalang, Ulysses, Mahaney, Michael, Make, Barry, Manichaikul, Ani, Manning, Alisa, Manson, JoAnn, Martin, Lisa, Marton, Melissa, Mathai, Susan, Mathias, Rasika, May, Susanne, McArdle, Patrick, McDonald, Merry-Lynn, McFarland, Sean, McGarvey, Stephen, McGoldrick, Daniel, McHugh, Caitlin, McNeil, Becky, Mei, Hao, Meigs, James, Menon, Vipin, Mestroni, Luisa, Metcalf, Ginger, Meyers, Deborah A., Mignot, Emmanuel, Mikulla, Julie, Min, Nancy, Minear, Mollie, Minster, Ryan L., Mitchell, Braxton D., Moll, Matt, Momin, Zeineen, Montasser, May E., Montgomery, Courtney, Muzny, Donna, Mychaleckyj, Josyf C., Nadkarni, Girish, Naik, Rakhi, Naseri, Take, Natarajan, Pradeep, Nekhai, Sergei, Nelson, Sarah C., Neltner, Bonnie, Nessner, Caitlin, Nickerson, Deborah, Nkechinyere, Osuji, North, Kari, O'Connell, Jeff, O'Connor, Tim, Ochs-Balcom, Heather, Okwuonu, Geoffrey, Pack, Allan, Paik, David T., Palmer, Nicholette, Pankow, James, Papanicolaou, George, Parker, Cora, Peloso, Gina, Peralta, Juan Manuel, Perez, Marco, Perry, James, Peters, Ulrike, Peyser, Patricia, Phillips, Lawrence S., Pleiness, Jacob, Pollin, Toni, Post, Wendy, Powers Becker, Julia, Preethi Boorgula, Meher, Preuss, Michael, Psaty, Bruce, Qasba, Pankaj, Qiao, Dandi, Qin, Zhaohui, Rafaels, Nicholas, Raffield, Laura, Rajendran, Mahitha, Ramachandran, Vasan S., Rao, D.C., Rasmussen-Torvik, Laura, Ratan, Aakrosh, Redline, Susan, Reed, Robert, Reeves, Catherine, Regan, Elizabeth, Reiner, Alex, Reupena, Muagututi‘a Sefuiva, Rice, Ken, Rich, Stephen, Robillard, Rebecca, Robine, Nicolas, Roden, Dan, Roselli, Carolina, Rotter, Jerome, Ruczinski, Ingo, Runnels, Alexi, Russell, Pamela, Ruuska, Sarah, Ryan, Kathleen, Sabino, Ester Cerdeira, Saleheen, Danish, Salimi, Shabnam, Salvi, Sejal, Salzberg, Steven, Sandow, Kevin, Sankaran, Vijay G., Santibanez, Jireh, Schwander, Karen, Schwartz, David, Sciurba, Frank, Seidman, Christine, Seidman, Jonathan, Sériès, Frédéric, Sheehan, Vivien, Sherman, Stephanie L., Shetty, Amol, Shetty, Aniket, Sheu, Wayne Hui-Heng, Shoemaker, M. Benjamin, Silver, Brian, Silverman, Edwin, Skomro, Robert, Smith, Albert Vernon, Smith, Jennifer, Smith, Josh, Smith, Nicholas, Smith, Tanja, Smoller, Sylvia, Snively, Beverly, Snyder, Michael, Sofer, Tamar, Sotoodehnia, Nona, Stilp, Adrienne M., Storm, Garrett, Streeten, Elizabeth, Su, Jessica Lasky, Sung, Yun Ju, Sylvia, Jody, Szpiro, Adam, Taliun, Daniel, Tang, Hua, Taub, Margaret, Taylor, Kent, Taylor, Matthew, Taylor, Simeon, Telen, Marilyn, Thornton, Timothy A., Threlkeld, Machiko, Tinker, Lesley, Tirschwell, David, Tishkoff, Sarah, Tiwari, Hemant, Tong, Catherine, Tracy, Russell, Tsai, Michael, Vaidya, Dhananjay, Van Den Berg, David, VandeHaar, Peter, Vrieze, Scott, Walker, Tarik, Wallace, Robert, Walts, Avram, Wang, Fei Fei, Wang, Heming, Wang, Jiongming, Watson, Karol, Watt, Jennifer, Weeks, Daniel E., Weinstock, Joshua, Weir, Bruce, Weiss, Scott T., Weng, Lu-Chen, Wessel, Jennifer, Willer, Cristen, Williams, Kayleen, Williams, L. Keoki, Williams, Scott, Wilson, Carla, Wilson, James, Winterkorn, Lara, Wong, Quenna, Wu, Baojun, Wu, Joseph, Xu, Huichun, Yanek, Lisa, Yang, Ivana, Yu, Ketian, Zekavat, Seyedeh Maryam, Zhang, Yingze, Zhao, Snow Xueyan, Zhao, Wei, Zhu, Xiaofeng, Ziv, Elad, Zody, Michael, Zoellner, Sebastian, Recto, Kathryn, Kachroo, Priyadarshini, Huan, Tianxiao, Lee, Gha Young, Bui, Helena, Lee, Dong Heon, Gereige, Jessica, Yao, Chen, Hwang, Shih-Jen, Joehanes, Roby, O’Connor, George T., and DeMeo, Dawn L.

Published
2023
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15. Multicenter clinical and functional evidence reclassifies a recurrent noncanonical filamin C splice-altering variant

Author

O’Neill, Matthew J., Chen, Suet Nee, Rumping, Lynne, Johnson, Renee, van Slegtenhorst, Marjon, Glazer, Andrew M., Yang, Tao, Solus, Joseph F., Laudeman, Julie, Mitchell, Devyn W., Vanags, Loren R., Kroncke, Brett M., Anderson, Katherine, Gao, Shanshan, Verdonschot, Job A.J., Brunner, Han, Hellebrekers, Debby, Taylor, Matthew R.G., Roden, Dan M., Wessels, Marja W., Lekanne Dit Deprez, Ronald H., Fatkin, Diane, Mestroni, Luisa, and Shoemaker, M. Benjamin

Published
2023
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16. Abstract 16898: Myocardial Recovery Associated Gene Cdcp1 Expression Attenuates Cardiac Fibrosis in a Pressure Over-Load Mouse Model

Author

Wang, Min, Vyas, Hridyanshu, Roos, Carolyn, Huang, Runqing, Liu, Duan, Sahni, Jyotan, McNamara, Dennis M, Barlera, Simona, Pileggi, Silvana, Mestroni, Luisa, Merlo, Marco, Sinagra, Gianfranco, Pinet, Florence, Chaloupka, Anna, Krejci, Jan, de GROOTE, Pascal, Weinshilboum, Richard, Miller, Jordan D, and Pereira, Naveen L

Published
2023
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18. Abstract 14249: Sex and Gene Based Differences in Age Related Penetrance of Dilated and Arrhythmogenic Cardiomyopathy

Author

Bart, Nicole K, Stroeks, Sophie, Khan, Sadiya S, Reza, Nosheen, Olivotto, Iacopo, Vissing, Christoffer, Sinnette, Corine, Owens, Anjali T, Fornaro, Alessandra, Mestroni, Luisa, Taylor, Matthew, Helms, Adam S, Prasad, Sanjay K, Saberi, Sara, Shore, Supriya, Verdonschot, Job, sinagra, Gianfranco, Merlo, Marco, Gigli, Marta, Wilsbacher, Lisa D, Heymans, Stephane, Wheeler, Matthew, Ashley, Euan A, Dal Ferro, Matteo, Paldino, Alessia, Day, Sharlene, de Feria, Alejandro, Stevenson, Lynne W, Ho, Carolyn, Claggett, Brian, Ware, James S, Parikh, Victoria N, Tayal, Upasana, and Lakdawala, Neal K

Published
2023
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20. Abstract 12135: Quality of Life in Early-Stage Hypertrophic Cardiomyopathy: A Secondary Analysis of the VANISH Trial

Author

Ireland, Catherine G, Day, Sharlene, Axelsson, Anna, Russell, Mark W, Zahka, Kenneth G, Pereira, Alexandre, Canter, Charles E, Bach, Richard G, Wheeler, Matthew T, Rossano, Joseph W, Owens, Anjali T, Bundgaard, Henning, Mestroni, Luisa, Taylor, Matthew R, Amit, Patel R, Wilmot, Ivan, Soslow, Jonathan H, Becker, Jason R, Orav, Endel J, and Ho, Carolyn Y

Published
2023
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21. Myocardial Injury and Altered Gene Expression Associated With SARS-CoV-2 Infection or mRNA Vaccination

Author

Altman, Natasha L., Berning, Amber A., Saxon, Cara E., Adamek, Kylie E., Wagner, Jessica A., Slavov, Dobromir, Quaife, Robert A., Gill, Edward A., Minobe, Wayne A., Jonas, Eric R., Carroll, Ian A., Huebler, Sophia P., Raines, Joshua, Messenger, John C., Ambardekar, Amrut V., Mestroni, Luisa, Rosenberg, Rachel M., Rove, Jessica, Campbell, Thomas B., and Bristow, Michael R.

Published
2023
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22. Altered microtubule structure, hemichannel localization and beating activity in cardiomyocytes expressing pathologic nuclear lamin A/C.

Author

Borin, Daniele, Peña, Brisa, Chen, Suet Nee, Long, Carlin S, Taylor, Matthew RG, Mestroni, Luisa, and Sbaizero, Orfeo

Subjects
Atomic force microscopy, Beating, Biological sciences, Biomechanics, Cardiology, Cardiomyocytes, Cardiomyopathy, Cx43, Cytoskeleton, Lamin A/C, Mechanical property, Membrane, Heart Disease, Bioengineering, Cardiovascular, 2.1 Biological and endogenous factors
Abstract

Given the clinical effect of laminopathies, understanding lamin mechanical properties will benefit the treatment of heart failure. Here we report a mechano-dynamic study of LMNA mutations in neonatal rat ventricular myocytes (NRVM) using single cell spectroscopy with Atomic Force Microscopy (AFM) and measured changes in beating force, frequency and contractile amplitude of selected mutant-expressing cells within cell clusters. Furthermore, since beat-to-beat variations can provide clues on the origin of arrhythmias, we analyzed the beating rate variability using a time-domain method which provides a Poincaré plot. Data were further correlated to cell phenotypes. Immunofluorescence and calcium imaging analysis showed that mutant lamin changed NRVMs beating force and frequency. Additionally, we noted an altered microtubule network organization with shorter filament length, and defective hemichannel membrane localization (Connexin 43). These data highlight the interconnection between nucleoskeleton, cytoskeleton and sarcolemmal structures, and the transcellular consequences of mutant lamin protein in the pathogenesis of the cardiac laminopathies.

Published
2020

23. Ankyrin-B dysfunction predisposes to arrhythmogenic cardiomyopathy and is amenable to therapy

Author

Roberts, Jason D, Murphy, Nathaniel P, Hamilton, Robert M, Lubbers, Ellen R, James, Cynthia A, Kline, Crystal F, Gollob, Michael H, Krahn, Andrew D, Sturm, Amy C, Musa, Hassan, El-Refaey, Mona, Koenig, Sara, Aneq, Meriam Åström, Hoorntje, Edgar T, Graw, Sharon L, Davies, Robert W, Rafiq, Muhammad Arshad, Koopmann, Tamara T, Aafaqi, Shabana, Fatah, Meena, Chiasson, David A, Taylor, Matthew RG, Simmons, Samantha L, Han, Mei, van Opbergen, Chantal JM, Wold, Loren E, Sinagra, Gianfranco, Mittal, Kirti, Tichnell, Crystal, Murray, Brittney, Codima, Alberto, Nazer, Babak, Nguyen, Duy T, Marcus, Frank I, Sobriera, Nara, Lodder, Elisabeth M, van den Berg, Maarten P, Spears, Danna A, Robinson, John F, Ursell, Philip C, Green, Anna K, Skanes, Allan C, Tang, Anthony S, Gardner, Martin J, Hegele, Robert A, van Veen, Toon AB, Wilde, Arthur AM, Healey, Jeff S, Janssen, Paul ML, Mestroni, Luisa, van Tintelen, J Peter, Calkins, Hugh, Judge, Daniel P, Hund, Thomas J, Scheinman, Melvin M, and Mohler, Peter J

Subjects
Heart Disease, Cardiovascular, Clinical Research, Rare Diseases, 2.1 Biological and endogenous factors, Aetiology, Animals, Ankyrins, Arrhythmogenic Right Ventricular Dysplasia, Disease Models, Animal, Female, Humans, Indoles, Male, Maleimides, Mice, Mice, Knockout, Myocardium, Wnt Signaling Pathway, beta Catenin, Arrhythmias, Cardiology, Cardiovascular disease, Cell Biology, Genetic diseases, Medical and Health Sciences, Immunology
Abstract

Arrhythmogenic cardiomyopathy (ACM) is an inherited arrhythmia syndrome characterized by severe structural and electrical cardiac phenotypes, including myocardial fibrofatty replacement and sudden cardiac death. Clinical management of ACM is largely palliative, owing to an absence of therapies that target its underlying pathophysiology, which stems partially from our limited insight into the condition. Following identification of deceased ACM probands possessing ANK2 rare variants and evidence of ankyrin-B loss of function on cardiac tissue analysis, an ANK2 mouse model was found to develop dramatic structural abnormalities reflective of human ACM, including biventricular dilation, reduced ejection fraction, cardiac fibrosis, and premature death. Desmosomal structure and function appeared preserved in diseased human and murine specimens in the presence of markedly abnormal β-catenin expression and patterning, leading to identification of a previously unknown interaction between ankyrin-B and β-catenin. A pharmacological activator of the WNT/β-catenin pathway, SB-216763, successfully prevented and partially reversed the murine ACM phenotypes. Our findings introduce what we believe to be a new pathway for ACM, a role of ankyrin-B in cardiac structure and signaling, a molecular link between ankyrin-B and β-catenin, and evidence for targeted activation of the WNT/β-catenin pathway as a potential treatment for this disease.

Published
2019

28. Biomechanical defects and rescue of cardiomyocytes expressing pathologic nuclear lamins

Author

Laurini, Erik, Martinelli, Valentina, Lanzicher, Thomas, Puzzi, Luca, Borin, Daniele, Chen, Suet Nee, Long, Carlin S, Lee, Patrice, Mestroni, Luisa, Taylor, Matthew RG, Sbaizero, Orfeo, and Pricl, Sabrina

Subjects
Biomedical and Clinical Sciences, Cardiovascular Medicine and Haematology, Cardiovascular, Genetics, Bioengineering, Heart Disease, 5.1 Pharmaceuticals, Development of treatments and therapeutic interventions, Aetiology, 2.1 Biological and endogenous factors, Animals, Animals, Newborn, Biomechanical Phenomena, Cardiomyopathies, Cells, Cultured, Elastic Modulus, Fluorescent Antibody Technique, Genetic Predisposition to Disease, Humans, Lamin Type A, Microscopy, Atomic Force, Molecular Dynamics Simulation, Mutation, Myocardial Contraction, Myocytes, Cardiac, Phenotype, Protein Conformation, alpha-Helical, Protein Kinase Inhibitors, Rats, Structure-Activity Relationship, p38 Mitogen-Activated Protein Kinases, Cardiorespiratory Medicine and Haematology, Cardiovascular System & Hematology, Cardiovascular medicine and haematology
Abstract

AimsGiven the clinical impact of LMNA cardiomyopathies, understanding lamin function will fulfill a clinical need and will lead to advancement in the treatment of heart failure. A multidisciplinary approach combining cell biology, atomic force microscopy (AFM), and molecular modeling was used to analyse the biomechanical properties of human lamin A/C gene (LMNA) mutations (E161K, D192G, N195K) using an in vitro neonatal rat ventricular myocyte model.Methods and resultsThe severity of biomechanical defects due to the three LMNA mutations correlated with the severity of the clinical phenotype. AFM and molecular modeling identified distinctive biomechanical and structural changes, with increasing severity from E161K to N195K and D192G, respectively. Additionally, the biomechanical defects were rescued with a p38 MAPK inhibitor.ConclusionsAFM and molecular modeling were able to quantify distinct biomechanical and structural defects in LMNA mutations E161K, D192G, and N195K and correlate the defects with clinical phenotypic severity. Improvements in cellular biomechanical phenotype was demonstrated and may represent a mechanism of action for p38 MAPK inhibition therapy that is now being used in human clinical trials to treat laminopathies.

Published
2018

29. An International Longitudinal Natural History Study of Danon Disease Patients: Unique Cardiac Trajectories Identified Based on Sex and Heart Failure Outcomes

Author

Hong, Kimberly N., primary, Eshraghian, Emily, additional, Khedro, Tarek, additional, Argirò, Alessia, additional, Attias, Jennifer, additional, Storm, Garrett, additional, Tsotras, Melina, additional, Bloks, Tanner, additional, Jackson, Isaiah, additional, Ahmad, Elijah, additional, Graw, Sharon, additional, Mestroni, Luisa, additional, Bui, Quan M., additional, Schwartz, Jonathan, additional, Turner, Stuart, additional, Adler, Eric D., additional, and Taylor, Matthew, additional

Published
2024
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33. The genetic architecture of Plakophilin 2 cardiomyopathy

Author

Dries, Annika M., Kirillova, Anna, Reuter, Chloe M., Garcia, John, Zouk, Hana, Hawley, Megan, Murray, Brittney, Tichnell, Crystal, Pilichou, Kalliopi, Protonotarios, Alexandros, Medeiros-Domingo, Argelia, Kelly, Melissa A., Baras, Aris, Ingles, Jodie, Semsarian, Christopher, Bauce, Barbara, Celeghin, Rudy, Basso, Cristina, Jongbloed, Jan D.H., Nussbaum, Robert L., Funke, Birgit, Cerrone, Marina, Mestroni, Luisa, Taylor, Matthew R.G., Sinagra, Gianfranco, Merlo, Marco, Saguner, Ardan M., Elliott, Perry M., Syrris, Petros, van Tintelen, J. Peter, James, Cynthia A., Haggerty, Christopher M., and Parikh, Victoria N.

Published
2021
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35. Right precordial-directed electrocardiographical markers identify arrhythmogenic right ventricular cardiomyopathy in the absence of conventional depolarization or repolarization abnormalities.

Author

Cortez, Daniel, Svensson, Anneli, Carlson, Jonas, Graw, Sharon, Sharma, Nandita, Brun, Francesca, Spezzacatene, Anita, Mestroni, Luisa, and Platonov, Pyotr

Subjects
Arrhythmogenic right ventricular cardiomyopathy, Cascade screening, ECG, Vectorcardiography, Adult, Arrhythmogenic Right Ventricular Dysplasia, Cohort Studies, Cross-Sectional Studies, Electrocardiography, Female, Humans, Internationality, Male, Middle Aged, Retrospective Studies, Young Adult
Abstract

BACKGROUND: Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) carries a risk of sudden death. We aimed to assess whether vectorcardiographic (VCG) parameters directed toward the right heart and a measured angle of the S-wave would help differentiate ARVD/C with otherwise normal electrocardiograms from controls. METHODS: Task Force 2010 definite ARVD/C criteria were met for all patients. Those who did not fulfill Task Force depolarization or repolarization criteria (-ECG) were compared with age and gender-matched control subjects. Electrocardiogram measures of a 3-dimentional spatial QRS-T angle, a right-precordial-directed orthogonal QRS-T (RPD) angle, a root mean square of the right sided depolarizing forces (RtRMS-QRS), QRS duration (QRSd) and the corrected QT interval (QTc), and a measured angle including the upslope and downslope of the S-wave (S-wave angle) were assessed. RESULTS: Definite ARVD/C was present in 155 patients by 2010 Task Force criteria (41.7 ± 17.6 years, 65.2% male). -ECG ARVD/C patients (66 patients) were compared to 66 control patients (41.7 ± 17.6 years, 65.2% male). All parameters tested except the QRSd and QTc significantly differentiated -ECG ARVD/C from control patients (p

Published
2017

36. Injectable Carbon Nanotube-Functionalized Reverse Thermal Gel Promotes Cardiomyocytes Survival and Maturation

Author

Peña, Brisa, Bosi, Susanna, Aguado, Brian A, Borin, Daniele, Farnsworth, Nikki L, Dobrinskikh, Evgenia, Rowland, Teisha J, Martinelli, Valentina, Jeong, Mark, Taylor, Matthew RG, Long, Carlin S, Shandas, Robin, Sbaizero, Orfeo, Prato, Maurizio, Anseth, Kristi S, Park, Daewon, and Mestroni, Luisa

Subjects
Engineering, Nanotechnology, Biomedical Engineering, Bioengineering, Cardiovascular, Regenerative Medicine, Heart Disease, Animals, Gelatin, Myocytes, Cardiac, Nanotubes, Carbon, Rats, Tissue Engineering, Tissue Scaffolds, carbon nanotube, cardiac tissue engineering, hybrid-biomimetic hydrogel, injectable polymer, reverse thermal gel, Chemical Sciences, Nanoscience & Nanotechnology, Chemical sciences, Physical sciences
Abstract

The ability of the adult heart to regenerate cardiomyocytes (CMs) lost after injury is limited, generating interest in developing efficient cell-based transplantation therapies. Rigid carbon nanotubes (CNTs) scaffolds have been used to improve CMs viability, proliferation, and maturation, but they require undesirable invasive surgeries for implantation. To overcome this limitation, we developed an injectable reverse thermal gel (RTG) functionalized with CNTs (RTG-CNT) that transitions from a solution at room temperature to a three-dimensional (3D) gel-based matrix shortly after reaching body temperature. Here we show experimental evidence that this 3D RTG-CNT system supports long-term CMs survival, promotes CMs alignment and proliferation, and improves CMs function when compared with traditional two-dimensional gelatin controls and 3D plain RTG system without CNTs. Therefore, our injectable RTG-CNT system could potentially be used as a minimally invasive tool for cardiac tissue engineering efforts.

Published
2017

37. Quality of Life and Exercise Capacity in Early Stage and Subclinical Hypertrophic Cardiomyopathy: A Secondary Analysis of the VANISH Trial.

Author

Ireland, Catherine G., Burstein, Danielle S., Day, Sharlene M., Raja, Anna Axelsson, Russell, Mark W., Zahka, Kenneth G., Pereira, Alexandre, Canter, Charles E., Bach, Richard G., Wheeler, Matthew T., Rossano, Joseph W., Owens, Anjali T., Bundgaard, Henning, Mestroni, Luisa, Taylor, Matthew R. G., Patel, Amit R., Wilmot, Ivan, Soslow, Jonathan H., Becker, Jason R., and Giverts, Ilya

Abstract

BACKGROUND: The health-related quality of life (HRQOL) and cardiopulmonary exercise testing (CPET) performance of individuals with subclinical and early stage hypertrophic cardiomyopathy (HCM) have not been systematically studied. Improved understanding will inform the natural history of HCM and factors influencing well-being. METHODS: VANISH trial (Valsartan for Attenuating Disease Evolution in Early Sarcomeric HCM) participants with early stage sarcomeric HCM (primary analysis cohort) and subclinical HCM (sarcomere variant without left ventricular hypertrophy comprising the exploratory cohort) who completed baseline and year 2 HRQOL assessment via the pediatric quality of life inventory and CPET were studied. Metrics correlating with baseline HRQOL and CPET performance were identified. The impact of valsartan treatment on these measures was analyzed in the early stage cohort. RESULTS: Two hundred participants were included: 166 with early stage HCM (mean age, 23±10 years; 40% female; 97% White; and 92% New York Heart Association class I) and 34 subclinical sarcomere variant carriers (mean age, 16±5 years; 50% female; and 100% White). Baseline HRQOL was good in both cohorts, although slightly better in subclinical HCM (composite pediatric quality of life score 84.6±10.6 versus 90.2±9.8; P=0.005). Both cohorts demonstrated mildly reduced functional status (mean percent predicted peak oxygen uptake 73±16 versus 78±12 mL/kg per minute; P=0.18). Percent predicted peak oxygen uptake and peak oxygen pulse correlated with HRQOL. Valsartan improved physical HRQOL in early stage HCM (adjusted mean change in pediatric quality of life score +4.1 versus placebo; P=0.01) but did not significantly impact CPET performance. CONCLUSIONS: Functional capacity can be impaired in young, healthy people with early stage HCM, despite New York Heart Association class I status and good HRQOL. Peak oxygen uptake was similarly decreased in subclinical HCM despite normal left ventricular wall thickness and excellent HRQOL. Valsartan improved physical pediatric quality of life scores but did not significantly impact CPET performance. Further studies are needed for validation and to understand how to improve patient experience. [ABSTRACT FROM AUTHOR]

Published
2024
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41. Biomimetic Polymers for Cardiac Tissue Engineering

Author

Peña, Brisa, Martinelli, Valentina, Jeong, Mark, Bosi, Susanna, Lapasin, Romano, Taylor, Matthew RG, Long, Carlin S, Shandas, Robin, Park, Daewon, and Mestroni, Luisa

Subjects
Regenerative Medicine, Heart Disease, Bioengineering, Cardiovascular, Biotechnology, 5.2 Cellular and gene therapies, Development of treatments and therapeutic interventions, Animals, Animals, Newborn, Biomimetics, Cells, Cultured, Embryonic Stem Cells, Laminin, Lysine, Male, Myocytes, Cardiac, Polymers, Rats, Rats, Sprague-Dawley, Tissue Engineering, Chemical Sciences, Biological Sciences, Engineering
Abstract

Heart failure is a morbid disorder characterized by progressive cardiomyocyte (CM) dysfunction and death. Interest in cell-based therapies is growing, but sustainability of injected CMs remains a challenge. To mitigate this, we developed an injectable biomimetic Reverse Thermal Gel (RTG) specifically engineered to support long-term CM survival. This RTG biopolymer provided a solution-based delivery vehicle of CMs, which transitioned to a gel-based matrix shortly after reaching body temperature. In this study we tested the suitability of this biopolymer to sustain CM viability. The RTG was biomolecule-functionalized with poly-l-lysine or laminin. Neonatal rat ventricular myocytes (NRVM) and adult rat ventricular myocytes (ARVM) were cultured in plain-RTG and biomolecule-functionalized-RTG both under 3-dimensional (3D) conditions. Traditional 2D biomolecule-coated dishes were used as controls. We found that the RTG-lysine stimulated NRVM to spread and form heart-like functional syncytia. Regarding cell contraction, in both RTG and RTG-lysine, beating cells were recorded after 21 days. Additionally, more than 50% (p value < 0.05; n = 5) viable ARVMs, characterized by a well-defined cardiac phenotype represented by sarcomeric cross-striations, were found in the RTG-laminin after 8 days. These results exhibit the tremendous potential of a minimally invasive CM transplantation through our designed RTG-cell therapy platform.

Published
2016

42. A novel tool for arrhythmic risk stratification in desmoplakin gene variant carriers

Author

Electrofysiologie, Genetica Sectie Genoomdiagnostiek, Circulatory Health, Genetica Groep Van Tintelen, Child Health, Team Medisch, Carrick, Richard T, Gasperetti, Alessio, Protonotarios, Alexandros, Murray, Brittney, Laredo, Mikael, van der Schaaf, Iris, Dooijes, Dennis, Syrris, Petros, Cannie, Douglas, Tichnell, Crystal, Gilotra, Nisha A, Cappelletto, Chiara, Medo, Kristen, Saguner, Ardan M, Duru, Firat, Hylind, Robyn J, Abrams, Dominic J, Lakdawala, Neal K, Cadrin-Tourigny, Julia, Targetti, Mattia, Olivotto, Iacopo, Graziosi, Maddalena, Cox, Moniek, Biagini, Elena, Charron, Philippe, Compagnucci, Paolo, Casella, Michela, Conte, Giulio, Tondo, Claudio, Yazdani, Momina, Ware, James S, Prasad, Sanjay K, Calò, Leonardo, Smith, Eric D, Helms, Adam S, Hespe, Sophie, Ingles, Jodie, Tandri, Harikrishna, Ader, Flavie, Peretto, Giovanni, Peters, Stacey, Horton, Ari, Yao, Jessica, Schulze-Bahr, Eric, Dittman, Sven, Carruth, Eric D, Young, Katelyn, Qureshi, Maria, Haggerty, Chris, Parikh, Victoria N, Taylor, Matthew, Mestroni, Luisa, Wilde, Arthur, Sinagra, Gianfranco, Merlo, Marco, Gandjbakhch, Estelle, van Tintelen, J Peter, Te Riele, Anneline S J M, Elliot, Perry, Calkins, Hugh, Wu, Katherine C, James, Cynthia A, Electrofysiologie, Genetica Sectie Genoomdiagnostiek, Circulatory Health, Genetica Groep Van Tintelen, Child Health, Team Medisch, Carrick, Richard T, Gasperetti, Alessio, Protonotarios, Alexandros, Murray, Brittney, Laredo, Mikael, van der Schaaf, Iris, Dooijes, Dennis, Syrris, Petros, Cannie, Douglas, Tichnell, Crystal, Gilotra, Nisha A, Cappelletto, Chiara, Medo, Kristen, Saguner, Ardan M, Duru, Firat, Hylind, Robyn J, Abrams, Dominic J, Lakdawala, Neal K, Cadrin-Tourigny, Julia, Targetti, Mattia, Olivotto, Iacopo, Graziosi, Maddalena, Cox, Moniek, Biagini, Elena, Charron, Philippe, Compagnucci, Paolo, Casella, Michela, Conte, Giulio, Tondo, Claudio, Yazdani, Momina, Ware, James S, Prasad, Sanjay K, Calò, Leonardo, Smith, Eric D, Helms, Adam S, Hespe, Sophie, Ingles, Jodie, Tandri, Harikrishna, Ader, Flavie, Peretto, Giovanni, Peters, Stacey, Horton, Ari, Yao, Jessica, Schulze-Bahr, Eric, Dittman, Sven, Carruth, Eric D, Young, Katelyn, Qureshi, Maria, Haggerty, Chris, Parikh, Victoria N, Taylor, Matthew, Mestroni, Luisa, Wilde, Arthur, Sinagra, Gianfranco, Merlo, Marco, Gandjbakhch, Estelle, van Tintelen, J Peter, Te Riele, Anneline S J M, Elliot, Perry, Calkins, Hugh, Wu, Katherine C, and James, Cynthia A

Published
2024

43. Long-Term Arrhythmic Follow-Up and Risk Stratification of Patients With Desmoplakin-Associated Arrhythmogenic Right Ventricular Cardiomyopathy

Author

Team Onderzoek, Circulatory Health, Genetica, Genetica Groep Van Tintelen, Cancer, Child Health, Gasperetti, Alessio, Carrick, Richard, Protonotarios, Alexandros, Laredo, Mikael, van der Schaaf, Iris, Syrris, Petros, Murray, Brittney, Tichnell, Crystal, Cappelletto, Chiara, Gigli, Marta, Medo, Kristen, Crabtree, Peter, Saguner, Ardan M., Duru, Firat, Hylind, Robyn, Abrams, Dominic, Lakdawala, Neal K., Massie, Charles, Cadrin-Tourigny, Julia, Targetti, Mattia, Olivotto, Iacopo, Graziosi, Maddalena, Cox, Moniek, Biagini, Elena, Charron, Philippe, Casella, Michela, Tondo, Claudio, Yazdani, Momina, Ware, James S., Prasad, Sanjay, Calò, Leonardo, Smith, Eric, Helms, Adam, Hespe, Sophie, Ingles, Jodie, Tandri, Harikrishna, Ader, Flavie, Mestroni, Luisa, Wilde, Arthur, Merlo, Marco, Gandjbakhch, Estelle, Calkins, Hugh, te Riele, Anneline S.J.M., Peter van Tintelen, J., Elliot, Perry, James, Cynthia A., Team Onderzoek, Circulatory Health, Genetica, Genetica Groep Van Tintelen, Cancer, Child Health, Gasperetti, Alessio, Carrick, Richard, Protonotarios, Alexandros, Laredo, Mikael, van der Schaaf, Iris, Syrris, Petros, Murray, Brittney, Tichnell, Crystal, Cappelletto, Chiara, Gigli, Marta, Medo, Kristen, Crabtree, Peter, Saguner, Ardan M., Duru, Firat, Hylind, Robyn, Abrams, Dominic, Lakdawala, Neal K., Massie, Charles, Cadrin-Tourigny, Julia, Targetti, Mattia, Olivotto, Iacopo, Graziosi, Maddalena, Cox, Moniek, Biagini, Elena, Charron, Philippe, Casella, Michela, Tondo, Claudio, Yazdani, Momina, Ware, James S., Prasad, Sanjay, Calò, Leonardo, Smith, Eric, Helms, Adam, Hespe, Sophie, Ingles, Jodie, Tandri, Harikrishna, Ader, Flavie, Mestroni, Luisa, Wilde, Arthur, Merlo, Marco, Gandjbakhch, Estelle, Calkins, Hugh, te Riele, Anneline S.J.M., Peter van Tintelen, J., Elliot, Perry, and James, Cynthia A.

Published
2024

46. AFM single-cell force spectroscopy links altered nuclear and cytoskeletal mechanics to defective cell adhesion in cardiac myocytes with a nuclear lamin mutation

Author

Lanzicher, Thomas, Martinelli, Valentina, Long, Carlin S, Del Favero, Giorgia, Puzzi, Luca, Borelli, Massimo, Mestroni, Luisa, Taylor, Matthew RG, and Sbaizero, Orfeo

Subjects
Medical Physiology, Biomedical and Clinical Sciences, Heart Disease, Bioengineering, Cardiovascular, Genetics, Aetiology, 2.1 Biological and endogenous factors, Animals, Animals, Newborn, Biomechanical Phenomena, Cell Adhesion, Cell Nucleus, Cell Shape, Cytoskeleton, Elastic Modulus, Humans, Lamin Type A, Microscopy, Atomic Force, Models, Biological, Mutant Proteins, Mutation, Myocytes, Cardiac, Rats, Wistar, Spectrum Analysis, Tubulin, Viscosity, AFM, cardiomyopathy, cardiomyocytes, cell physiology, lamin A/C, relaxation force test, Young Modulus
Abstract

Previous investigations suggested that lamin A/C gene (LMNA) mutations, which cause a variety of human diseases including muscular dystrophies and cardiomyopathies, alter the nuclear mechanical properties. We hypothesized that biomechanical changes may extend beyond the nucleus.

Published
2015

47. The Cardiomyopathy Lamin A/C D192G Mutation Disrupts Whole-Cell Biomechanics in Cardiomyocytes as Measured by Atomic Force Microscopy Loading-Unloading Curve Analysis.

Author

Lanzicher, Thomas, Martinelli, Valentina, Puzzi, Luca, Del Favero, Giorgia, Codan, Barbara, Long, Carlin S, Mestroni, Luisa, Taylor, Matthew RG, and Sbaizero, Orfeo

Subjects
Cells, Cultured, Cell Nucleus, Myocytes, Cardiac, Animals, Humans, Rats, Cardiomyopathies, Cytochalasin D, Lamin Type A, Microscopy, Atomic Force, Cell Adhesion, Mutagenesis, Elastic Modulus, Cells, Cultured, Myocytes, Cardiac, Microscopy, Atomic Force
Abstract

Atomic force microscopy (AFM) cell loading/unloading curves were used to provide comprehensive insights into biomechanical behavior of cardiomyocytes carrying the lamin A/C (LMNA) D192G mutation known to cause defective nuclear wall, myopathy and severe cardiomyopathy. Our results suggested that the LMNA D192G mutation increased maximum nuclear deformation load, nuclear stiffness and fragility as compared to controls. Furthermore, there seems to be a connection between this lamin nuclear mutation and cell adhesion behavior since LMNA D192G cardiomyocytes displayed loss of AFM probe-to-cell membrane adhesion. We believe that this loss of adhesion involves the cytoskeletal architecture since our microscopic analyses highlighted that mutant LMNA may also lead to a morphological alteration in the cytoskeleton. Furthermore, chemical disruption of the actin cytoskeleton by cytochalasin D in control cardiomyocytes mirrored the alterations in the mechanical properties seen in mutant cells, suggesting a defect in the connection between the nucleoskeleton, cytoskeleton and cell adhesion molecules in cells expressing the mutant protein. These data add to our understanding of potential mechanisms responsible for this fatal cardiomyopathy, and show that the biomechanical effects of mutant lamin extend beyond nuclear mechanics to include interference of whole-cell biomechanical properties.

Published
2015

48. 2019 HRS expert consensus statement on evaluation, risk stratification, and management of arrhythmogenic cardiomyopathy: Executive summary

Author

Towbin, Jeffrey A., McKenna, William J., Abrams, Dominic J., Ackerman, Michael J., Calkins, Hugh, Darrieux, Francisco C.C., Daubert, James P., de Chillou, Christian, DePasquale, Eugene C., Desai, Milind Y., Estes, N.A. Mark, III, Hua, Wei, Indik, Julia H., Ingles, Jodie, James, Cynthia A., John, Roy M., Judge, Daniel P., Keegan, Roberto, Krahn, Andrew D., Link, Mark S., Marcus, Frank I., McLeod, Christopher J., Mestroni, Luisa, Priori, Silvia G., Saffitz, Jeffrey E., Sanatani, Shubhayan, Shimizu, Wataru, van Tintelen, J. Peter, Wilde, Arthur A.M., and Zareba, Wojciech

Published
2019
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