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4. Monitoring Biochemical Changes of Neuroblastoma Cells in Early Stages After X-Ray Exposure by Using Fourier-Transform Infrared Spectroscopy.

Author

Esposito, Rosario, Portaccio, Marianna, Meschini, Roberta, Delfino, Ines, and Lepore, Maria

Abstract

X-ray radiation treatments are largely adopted in radiotherapy, and Fourier-transform infrared microspectroscopy (μ-FTIR) has already been demonstrated to be a useful instrument for monitoring radiotherapy effects. Previous works in this field have focused on studying the changes occurring in cells when they are fixed immediately after the irradiation or 24 and 48 h later. In the present paper, changes occurring in SH-SY5Y neuroblastoma cells in the first hours after the irradiation are examined to obtain information on the processes taking place in this not-yet-investigated time window by using μ-FTIR. For this purpose, cell samples were fixed immediately after X-ray exposure, and 2 and 4 h after irradiation and investigated along with unexposed cells. Different data analysis procedures were implemented to estimate the changes in lipid, protein, and DNA spectral contributions. The present investigation on the effects of X-ray in the first hours after the exposure is helpful for better describing the processes occurring in this time window that offer the possibility of a timely check on the efficacy of X-ray treatments and can potentially be applied for planning personalized treatment as required by the most advanced medical therapy. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Young transgenic hMTH1 mice are protected against dietary fat‐induced metabolic stress—implications for enhanced longevity

Author

Marcon, Francesca, primary, Meschini, Roberta, additional, Iorio, Egidio, additional, Palleschi, Simonetta, additional, De Luca, Gabriele, additional, Siniscalchi, Ester, additional, Conti, Luigi, additional, Chirico, Mattea, additional, Pisanu, Maria Elena, additional, De Battistis, Francesca, additional, Rossi, Barbara, additional, Minoprio, Anna, additional, Giuliani, Alessandro, additional, Karran, Peter, additional, and Bignami, Margherita, additional

Published
2022
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19. RENEB Inter-Laboratory comparison 2017 : limits and pitfalls of ILCs

Author

Gregoire, Eric, Barquinero, Joan Francesc, Gruel, Gaetan, Benadjaoud, Mohamedamine, Martinez, Juan S., Beinke, Christina, Balajee, Adayabalam, Beukes, Philip, Blakely, William F., Dominguez, Inmaculada, Duy, Pham Ngoc, Gil, Octávia Monteiro, Güçlü, Inci, Guogyte, Kamile, Hadjidekova, Savina Petrova, Hadjidekova, Valeria, Hande, Prakash, Jang, Seongjae, Lumniczky, Katalin, Meschini, Roberta, Milic, Mirta, Montoro, Alegria, Moquet, Jayne, Moreno, Mercedes, Norton, Farrah N., Oestreicher, Ursula, Pajic, Jelena, Sabatier, Laure, Sommer, Sylwester, Testa, Antonella, Terzoudi, Georgia, Valente, Marco, Venkatachalam, Perumal, Vral, Anne, Wilkins, Ruth C., Wojcik, Andrzej, Zafiropoulos, Demetre, Kulka, Ulrike, Gregoire, Eric, Barquinero, Joan Francesc, Gruel, Gaetan, Benadjaoud, Mohamedamine, Martinez, Juan S., Beinke, Christina, Balajee, Adayabalam, Beukes, Philip, Blakely, William F., Dominguez, Inmaculada, Duy, Pham Ngoc, Gil, Octávia Monteiro, Güçlü, Inci, Guogyte, Kamile, Hadjidekova, Savina Petrova, Hadjidekova, Valeria, Hande, Prakash, Jang, Seongjae, Lumniczky, Katalin, Meschini, Roberta, Milic, Mirta, Montoro, Alegria, Moquet, Jayne, Moreno, Mercedes, Norton, Farrah N., Oestreicher, Ursula, Pajic, Jelena, Sabatier, Laure, Sommer, Sylwester, Testa, Antonella, Terzoudi, Georgia, Valente, Marco, Venkatachalam, Perumal, Vral, Anne, Wilkins, Ruth C., Wojcik, Andrzej, Zafiropoulos, Demetre, and Kulka, Ulrike

Abstract

Purpose: In case of a mass-casualty radiological event, there would be a need for networking to overcome surge limitations and to quickly obtain homogeneous results (reported aberration frequencies or estimated doses) among biodosimetry laboratories. These results must be consistent within such network. Inter-laboratory comparisons (ILCs) are widely accepted to achieve this homogeneity. At the European level, a great effort has been made to harmonize biological dosimetry laboratories, notably during the MULTIBIODOSE and RENEB projects. In order to continue the harmonization efforts, the RENEB consortium launched this intercomparison which is larger than the RENEB network, as it involves 38 laboratories from 21 countries. In this ILC all steps of the process were monitored, from blood shipment to dose estimation. This exercise also aimed to evaluate the statistical tools used to compare laboratory performance. Materials and methods: Blood samples were irradiated at three different doses, 1.8, 0.4 and 0 Gy (samples A, C and B) with 4-MV X-rays at 0.5 Gy min−1, and sent to the participant laboratories. Each laboratory was requested to blindly analyze 500 cells per sample and to report the observed frequency of dicentric chromosomes per metaphase and the corresponding estimated dose. Results: This ILC demonstrates that blood samples can be successfully distributed among laboratories worldwide to perform biological dosimetry in case of a mass casualty event. Having achieved a substantial harmonization in multiple areas among the RENEB laboratories issues were identified with the available statistical tools, which are not capable to advantageously exploit the richness of results of a large ILCs. Even though Z- and U-tests are accepted methods for biodosimetry ILCs, setting the number of analyzed metaphases to 500 and establishing a tests’ common threshold for all studied doses is inappropriate for evaluating laboratory performance. Another problem highlighted by this ILC

Published
2021
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22. RENEB Inter-Laboratory comparison 2017: limits and pitfalls of ILCs

Author

Gregoire, Eric, primary, Barquinero, Joan Francesc, additional, Gruel, Gaetan, additional, Benadjaoud, Mohamedamine, additional, Martinez, Juan S., additional, Beinke, Christina, additional, Balajee, Adayabalam, additional, Beukes, Philip, additional, Blakely, William F., additional, Dominguez, Inmaculada, additional, Duy, Pham Ngoc, additional, Gil, Octávia Monteiro, additional, Güçlü, Inci, additional, Guogyte, Kamile, additional, Hadjidekova, Savina Petrova, additional, Hadjidekova, Valeria, additional, Hande, Prakash, additional, Jang, Seongjae, additional, Lumniczky, Katalin, additional, Meschini, Roberta, additional, Milic, Mirta, additional, Montoro, Alegria, additional, Moquet, Jayne, additional, Moreno, Mercedes, additional, Norton, Farrah N., additional, Oestreicher, Ursula, additional, Pajic, Jelena, additional, Sabatier, Laure, additional, Sommer, Sylwester, additional, Testa, Antonella, additional, Terzoudi, Georgia, additional, Valente, Marco, additional, Venkatachalam, Perumal, additional, Vral, Anne, additional, Wilkins, Ruth C., additional, Wojcik, Andrzej, additional, Zafiropoulos, Demetre, additional, and Kulka, Ulrike, additional

Published
2021
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23. mitochondrial poison carbonyl cyanide 3-chlorophenyl hydrazone (CCCP) induces aneugenic effects in primary human fibroblasts: a possible link between mitochondrial dysfunction and chromosomal loss.

Author

Marcon, Francesca, Battistis, Francesca De, Siniscalchi, Ester, Crebelli, Riccardo, and Meschini, Roberta

Subjects
CYANIDE poisoning, CELL morphology, MITOCHONDRIA, MITOCHONDRIAL DNA, CYTOGENETICS, CELL cycle, GENETIC toxicology
Abstract

An association between proper chromosome segregation and intact mitochondria has been extensively reported. This could be related to the effects on the progression of cell division of altered energy production, increased oxidative stress, and deregulated calcium homeostasis. However, evidence for a direct relationship is still lacking. The present study was aimed at investigating the possible effect of mitochondrial dysfunction on chromosomal instability as detected in primary human cells treated with the mitochondrial poison carbonyl cyanide 3-chlorophenyl hydrazone (CCCP). Chromosome instability was analyzed in anaphase and interphase cells to follow the fate of chromosome damage during the progression of mitosis and the subsequent cell cycle. Through the combination of cytogenetic approaches and molecular analyses, i.e. morphological cell analysis, formation and characterization of micronucleus content, Comet assay, and gene expression, it was demonstrated that the prevalent DNA damage associated with CCCP treatment was the induction of chromosome loss, while primary DNA damage was not detected. No alterations in the shape of anaphase cells were observed nor induction of multipolar spindles. The proper activation of mitotic checkpoint was maintained. A linear dose–response curve characterizing the CCCP effects suggested that multiple cellular targets could be affected by the CCCP-induced mitochondrial dysfunctions triggering aneuploidy. Conversely, a steep increase was induced by the positive control vinblastine, known to have tubulin as a unique target. In addition, the effect of CCCP on mitochondrial function was demonstrated by changes in mitochondrial DNA copy number and in the expression of genes involved in mitochondrial maintenance. Overall, these results indicate that the mitochondrial poison CCCP may induce aneugenic effects. [ABSTRACT FROM AUTHOR]

Published
2022
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26. DNA integrity and ecophysiological responses of Spanish populations of Ulmus glabra to increasing ozone levels.

Author

Dell'Orso, Ambra, Kuzminsky, Elena, Bermejo-Bermejo, Victoria, Ruiz-Checa, Raquel, Amo, Rocío Alonso-Del, and Meschini, Roberta

Subjects
OZONE, TROPOSPHERIC ozone, DNA, PLANT DNA, DNA damage
Abstract

Ulmus glabra is a deciduous tree with a wide distribution in the Eurosiberian region. The southernmost populations, in the Mediterranean area, are fragmented in mountain areas which act as a refugium. These small relict populations can act as sentinel of global change, including climate change and impacts of human activities such as air pollution. Besides, tropospheric ozone (O3) is an additional stress factor in the Mediterranean region affecting plant physiology and health. Moreover, oxidative stress caused by O3 could increase DNA damage in plants cells. U. glabra 4-year-old seedlings originated from a natural population growing in the Guadarrama mountain range (central Spain), were exposed in Open Top Chambers to four O3 treatments: charcoal filtered air, non-filtered air reproducing ambient levels, non-filtered air supplemented with 15 nl l−1 O3 and non- filtered air supplemented with 30 nl l−1 O3. Ozone effects on the DNA integrity through Comet assay were evaluated and eco-physiological responses were explored as well as. Comet assay showed a significant increase of DNA damage with increasing levels of O3 after only one-month exposure, when no eco-physiological symptoms of damage could be detected. Comet assay could thus be suggested as a predictive test to detect DNA damage induced in plants by other abiotic stresses as well as to identify tolerant and sensitive species or in preservation strategies of small relict populations. The discovery of a test for an early identification of stressed plants could be important to speed the selection of tolerant individuals for breeding programmes. [ABSTRACT FROM AUTHOR]

Published
2021
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29. Capabilities of the RENEB network for research and large scale radiological and nuclear emergency situations

Author

Monteiro Gil, Octavia, Pedro Vaz, Romm, Horst, Angelis, Cinzia, Antunes, Ana Catarina, Barquinero, Joan-Francesc, Beinke, Christina, Bortolin, Emanuela, Burbidge, Christopher Ian, Cucu, Alexandra, Della Monaca, Sara, Domene, Mercedes Moreno, Fattibene, Paola, Gregoire, Eric, Hadjidekova, Valeria, Kulka, Ulrike, Lindholm, Carita, Meschini, Roberta, M Kacher, Radhia, Moquet, Jayne, Oestreicher, Ursula, Palitti, Fabrizio, Pantelias, Gabriel, Montoro Pastor, Alegria, Popescu, Irina-Anca, Quattrini, Maria Cristina, Ricoul, Michelle, Rothkamm, Kai, Sabatier, Laure, Sebastia, Natividad, Sommer, Sylwester, Terzoudi, Georgia, Testa, Antonella, Trompier, Francois, Vral, Anne, Istituto Superiore di Sanita` (ISS), Bundeswehr Institute of Radiobiology, Universität Ulm - Ulm University [Ulm, Allemagne], Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Bundesamt für Strahlenschutz (BfS), Radiation and Nuclear Safety Authority [Helsinki] (STUK), University of Tuscia, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre for Radiation, Chemical and Environmental Hazards, Public Health England [London], Hospital Universitario y Politécnico La Fe, Agenzia Nazionale per le nuove Tecnologie, l’energia e lo sviluppo economico sostenibile (ENEA), Universiteit Gent = Ghent University [Belgium] (UGENT), Seventh Framework Programme, Istituto Superiore di Sanità (ISS), Bundesamt für Strahlenschutz - Federal Office for Radiation Protection (BfS), Università degli studi della Tuscia [Viterbo], Hospital Universitari i Politècnic La Fe = University and Polytechnic Hospital La Fe, Agenzia Nazionale per le nuove Tecnologie, l’energia e lo sviluppo economico sostenibile = Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), and Universiteit Gent = Ghent University (UGENT)

Subjects
BIODOSIMETRY RENEB, Safety Management, Biomedical Research, [SDV]Life Sciences [q-bio], questionnaire/survey, Radiobiology, Biology and Life Sciences, Biodosimetry, Disaster Planning, emergency preparedness, capacity of laboratory response, radiological accident/emergency, Europe, Radiation Protection, EUROPEAN NETWORK, Radiation Monitoring, Models, Organizational, Radioactive Hazard Release
Abstract

International audience; Purpose: To identify and assess, among the participants in the RENEB (Realizing the European Network of Biodosimetry) project, the emergency preparedness, response capabilities and resources that can be deployed in the event of a radiological or nuclear accident/incident affecting a large number of individuals. These capabilities include available biodosimetry techniques, infrastructure, human resources (existing trained staff), financial and organizational resources (including the role of national contact points and their articulation with other stakeholders in emergency response) as well as robust quality control/assurance systems. Materials and methods: A survey was prepared and sent to the RENEB partners in order to acquire information about the existing, operational techniques and infrastructure in the laboratories of the different RENEB countries and to assess the capacity of response in the event of radiological or nuclear accident involving mass casualties. The survey focused on several main areas: laboratory’s general information, country and staff involved in biological and physical dosimetry; retrospective assays used, the number of assays available per laboratory and other information related to biodosimetry and emergency preparedness. Following technical intercomparisons amongst RENEB members, an update of the survey was performed one year later concerning the staff and the available assays. Conclusions: The analysis of RENEB questionnaires allowed a detailed assessment of existing capacity of the RENEB network to respond to nuclear and radiological emergencies. This highlighted the key importance of international cooperation in order to guarantee an effective and timely response in the event of radiological or nuclear accidents involving a considerable number of casualties. The deployment of the scientific and technical capabilities existing within the RENEB network members seems mandatory, to help other countries with less or no capacity for biological or physical dosimetry, or countries overwhelmed in case of a radiological or nuclear accident involving a large number of individuals. © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Published
2017
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30. RENEB : running the European network of biological dosimetry and physical retrospective dosimetry

Author

Kulka, Ulrike, Abend, Michael, Ainsbury, Elizabeth, Badie, Christophe, Barquinero, Joan Francesc, Leonardo Barrios, Beinke, Christina, Bortolin, Emanuela, Cucu, Alexandra, Amicis, Andrea, Dominguez, Inmaculada, Fattibene, Paola, Frovig, Anne Marie, Gregoire, Eric, Guogyte, Kamile, Hadjidekova, Valeria, Jaworska, Alicja, Kriehuber, Ralf, Lindholm, Carita, Lloyd, David, Lumniczky, Katalin, Lyng, Fiona, Meschini, Roberta, Mortl, Simone, Della Monaca, Sara, Monteiro Gil, Octavia, Montoro, Alegria, Moquet, Jayne, Moreno, Mercedes, Oestreicher, Ursula, Palitti, Fabrizio, Pantelias, Gabriel, Patrono, Clarice, Piqueret-Stephan, Laure, Port, Matthias, Prieto, Maria Jesus, Quintens, Roel, Ricoul, Michelle, Romm, Horst, Roy, Laurence, Safrany, Geza, Sabatier, Laure, Sebastia, Natividad, Sommer, Sylwester, Terzoudi, Georgia, Testa, Antonella, Thierens, Hubert, Turai, Istvan, Trompier, Francois, Valente, Marco, Vaz, Pedro, Voisin, Philippe, Vral, Anne, Woda, Clemens, Zafiropoulos, Demetre, and Wojcik, Andrzej

Subjects
Biological dosimetry, BIODOSIMETRY RENEB, LABORATORIES, Safety Management, RADIOLOGICAL EMERGENCY, networking, Disaster Planning, emergency preparedness, physical retrospective dosimetry, REACTOR ACCIDENT, dose estimation, Radiation Protection, Radiation Monitoring, RADIATION EXPOSURE, Humans, Organizational Objectives, PREMATURELY CONDENSED CHROMOSOMES, Radiation Injuries, CASUALTIES, DICENTRIC CHROMOSOME ASSAY, CHERNOBYL, Biology and Life Sciences, TRIAGE, Radiation Exposure, Europe, Biological Assay, Emergencies, Radioactive Hazard Release, RENEB
Abstract

Purpose: A European network was initiated in 2012 by 23 partners from 16 European countries with the aim to significantly increase individualized dose reconstruction in case of large-scale radiological emergency scenarios. Results: The network was built on three complementary pillars: (1) an operational basis with seven biological and physical dosimetric assays in ready-to-use mode, (2) a basis for education, training and quality assurance, and (3) a basis for further network development regarding new techniques and members. Techniques for individual dose estimation based on biological samples and/or inert personalized devices as mobile phones or smart phones were optimized to support rapid categorization of many potential victims according to the received dose to the blood or personal devices. Communication and cross-border collaboration were also standardized. To assure long-term sustainability of the network, cooperation with national and international emergency preparedness organizations was initiated and links to radiation protection and research platforms have been developed. A legal framework, based on a Memorandum of Understanding, was established and signed by 27 organizations by the end of 2015. Conclusions: RENEB is a European Network of biological and physical-retrospective dosimetry, with the capacity and capability to perform large-scale rapid individualized dose estimation. Specialized to handle large numbers of samples, RENEB is able to contribute to radiological emergency preparedness and wider large-scale research projects.

Published
2017

31. Dose assessment intercomparisons within the RENEB network using G(0)-lymphocyte prematurely condensed chromosomes (PCC assay)

Author

Terzoudi, Georgia I, Pantelias, Gabriel, Darroudi, Firouz, Barszczewska, Katarzyna, Buraczewska, Iwona, Depuydt, Julie, Georgieva, Dimka, Hadjidekova, Valeria, Hatzi, Vasiliki I, Karachristou, Ioanna, Karakosta, Maria, Meschini, Roberta, M’Kacher, Radhia, Montoro, Alegria, Palitti, Fabrizio, Pantelias, Antonio, Pepe, Gaetano, Ricoul, Michelle, Sabatier, Laure, Sebastià, Natividad, Sommer, Sylwester, Vral, Anne, Zafiropoulos, Demetre, and Wojcik, Andrzej

Subjects
cell fusion, EXCHANGES, centromere/telomere PNA FISH, Biology and Life Sciences, Biodosimetry, IRRADIATED HUMAN-LYMPHOCYTES, PCC assay, PROBES, PERIPHERAL-BLOOD LYMPHOCYTES, FISH, TRIAGE BIODOSIMETRY, premature chromosome condensation, RADIATION, COMBINATION, BIOLOGICAL DOSIMETRY, FRAGMENTS
Abstract

Purpose: Dose assessment intercomparisons within the RENEB network were performed for triage biodosimetry analyzing G(0)-lymphocyte PCC for harmonization, standardization and optimization of the PCC assay. Materials and methods: Comparative analysis among different partners for dose assessment included shipment of PCC-slides and captured images to construct dose-response curves for up to 6 Gy gamma-rays. Accident simulation exercises were performed to assess the suitability of the PCC assay by detecting speed of analysis and minimum number of cells required for categorization of potentially exposed individuals. Results: Calibration data based on Giemsa-stained fragments in excess of 46 PCC were obtained by different partners using galleries of PCC images for each dose-point. Mean values derived from all scores yielded a linear dose-response with approximately 4 excess-fragments/cell/Gy. To unify scoring criteria, exercises were carried out using coded PCC-slides and/or coded irradiated blood samples. Analysis of samples received 24h post-exposure was successfully performed using Giemsa staining (1 excess-fragment/cell/Gy) or centromere/telomere FISH-staining for dicentrics. Conclusions: Dose assessments by RENEB partners using appropriate calibration curves were mostly in good agreement. The PCC assay is quick and reliable for whole- or partial-body triage biodosimetry by scoring excess-fragments or dicentrics in G(0)-lymphocytes. Particularly, analysis of Giemsa-stained excess PCC-fragments is simple, inexpensive and its automation could increase throughput and scoring objectivity of the PCC assay.

Published
2017

35. Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation

Author

Trompier, Francois, Baumann, Marion, Barrios, Lleonard, Gregoire, Eric, Abend, Michael, Ainsbury, Elizabeth, Barnard, Stephen, Barquinero, Joan Francesc, Antonio Bautista, Juan, Brzozowska, Beata, Perez-Calatayud, Jose, De Angelis, Cinzia, Dominguez, Inmaculada, Hadjidekova, Valeria, Kulka, Ulrike, Carlos Mateos, Juan, Meschini, Roberta, Gil, Octavia Monteiro, Moquet, Jayne, Oestreicher, Ursula, Montoro Pastor, Alegria, Quintens, Roel, Sebastia, Natividad, Sommer, Sylwester, Stoyanov, Orlin, Thierens, Hubert, Terzoudi, Georgia, Ignacio Villaescusa, Juan, Vral, Anne, Wojcik, Andrzej, Zafiropoulos, Demetre, Roy, Laurence, Trompier, Francois, Baumann, Marion, Barrios, Lleonard, Gregoire, Eric, Abend, Michael, Ainsbury, Elizabeth, Barnard, Stephen, Barquinero, Joan Francesc, Antonio Bautista, Juan, Brzozowska, Beata, Perez-Calatayud, Jose, De Angelis, Cinzia, Dominguez, Inmaculada, Hadjidekova, Valeria, Kulka, Ulrike, Carlos Mateos, Juan, Meschini, Roberta, Gil, Octavia Monteiro, Moquet, Jayne, Oestreicher, Ursula, Montoro Pastor, Alegria, Quintens, Roel, Sebastia, Natividad, Sommer, Sylwester, Stoyanov, Orlin, Thierens, Hubert, Terzoudi, Georgia, Ignacio Villaescusa, Juan, Vral, Anne, Wojcik, Andrzej, Zafiropoulos, Demetre, and Roy, Laurence

Abstract

Purpose: In the frame of the QA program of RENEB, an inter-laboratory comparison (ILC) of calibration sources used in biological dosimetry was achieved to investigate the influence of calibration practices and protocols on the results of the dose estimation performance as a first step to harmonization and standardization of dosimetry and irradiation practices in the European biological dosimetry network. Materials and methods: Delivered doses by irradiation facilities used by RENEB partners were determined with EPR/alanine dosimetry system. Dosimeters were irradiated in the same conditions as blood samples. A short survey was also performed to collect the information needed for the data analysis and evaluate the diversity of practices. Results: For most of partners the deviation of delivered dose from the targeted dose remains below 10%. Deviations larger than 10% were observed for five facilities out of 21. Origins of the largest discrepancies were identified. Correction actions were evaluated as satisfactory. The re-evaluation of some ILC results for the fluorescence in situ hybridization (FISH) and premature chromosome condensation (PCC) assays has been performed leading to an improvement of the overall performances. Conclusions: This work has shown the importance of dosimetry in radiobiology studies and the needs of harmonization, standardization in irradiation and dosimetry practices and educational training for biologists using ionizing radiation.

Published
2017
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36. RENEB accident simulation exercise

Author

Brzozowska, Beata, Ainsbury, Elizabeth, Baert, Annelot, Beaton-Green, Lindsay, Barrios, Leonardo, Francesc Barquinero, Joan, Bassinet, Celine, Beinke, Christina, Benedek, Anett, Beukes, Philip, Bortolin, Emanuela, Buraczewska, Iwona, Burbidge, Christopher, De Amicis, Andrea, De Angelis, Cinzia, Della Monaca, Sara, Depuydt, Julie, De Sanctis, Stefania, Dobos, Katalin, Domene, Mercedes Moreno, Dominguez, Inmaculada, Facco, Eva, Fattibene, Paola, Frenzel, Monika, Gil, Octavia Monteiro, Gonon, Geraldine, Gregoire, Eric, Gruel, Gaetan, Hadjidekova, Valeria, Hatzi, Vasiliki I., Hristova, Rositsa, Jaworska, Alicja, Kis, Eniko, Kowalska, Maria, Kulka, Ulrike, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Meschini, Roberta, Moertl, Simone, Moquet, Jayne, Noditi, Mihaela, Oestreicher, Ursula, Orta Vazquez, Manuel Luis, Palma, Valentina, Pantelias, Gabriel, Montoro Pastor, Alegria, Patrono, Clarice, Piqueret-Stephan, Laure, Quattrini, Maria Cristina, Regalbuto, Elisa, Ricoul, Michelle, Roch-Lefevre, Sandrine, Roy, Laurence, Sabatier, Laure, Sarchiapone, Lucia, Sebastia, Natividad, Sommer, Sylwester, Sun, Mingzhu, Suto, Yumiko, Terzoudi, Georgia, Trompier, Francois, Vral, Anne, Wilkins, Ruth, Zafiropoulos, Demetre, Wieser, Albrecht, Woda, Clemens, Wojcik, Andrzej, Brzozowska, Beata, Ainsbury, Elizabeth, Baert, Annelot, Beaton-Green, Lindsay, Barrios, Leonardo, Francesc Barquinero, Joan, Bassinet, Celine, Beinke, Christina, Benedek, Anett, Beukes, Philip, Bortolin, Emanuela, Buraczewska, Iwona, Burbidge, Christopher, De Amicis, Andrea, De Angelis, Cinzia, Della Monaca, Sara, Depuydt, Julie, De Sanctis, Stefania, Dobos, Katalin, Domene, Mercedes Moreno, Dominguez, Inmaculada, Facco, Eva, Fattibene, Paola, Frenzel, Monika, Gil, Octavia Monteiro, Gonon, Geraldine, Gregoire, Eric, Gruel, Gaetan, Hadjidekova, Valeria, Hatzi, Vasiliki I., Hristova, Rositsa, Jaworska, Alicja, Kis, Eniko, Kowalska, Maria, Kulka, Ulrike, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Meschini, Roberta, Moertl, Simone, Moquet, Jayne, Noditi, Mihaela, Oestreicher, Ursula, Orta Vazquez, Manuel Luis, Palma, Valentina, Pantelias, Gabriel, Montoro Pastor, Alegria, Patrono, Clarice, Piqueret-Stephan, Laure, Quattrini, Maria Cristina, Regalbuto, Elisa, Ricoul, Michelle, Roch-Lefevre, Sandrine, Roy, Laurence, Sabatier, Laure, Sarchiapone, Lucia, Sebastia, Natividad, Sommer, Sylwester, Sun, Mingzhu, Suto, Yumiko, Terzoudi, Georgia, Trompier, Francois, Vral, Anne, Wilkins, Ruth, Zafiropoulos, Demetre, Wieser, Albrecht, Woda, Clemens, and Wojcik, Andrzej

Abstract

Purpose: The RENEB accident exercise was carried out in order to train the RENEB participants in coordinating and managing potentially large data sets that would be generated in case of a major radiological event. Materials and methods: Each participant was offered the possibility to activate the network by sending an alerting email about a simulated radiation emergency. The same participant had to collect, compile and report capacity, triage categorization and exposure scenario results obtained from all other participants. The exercise was performed over 27 weeks and involved the network consisting of 28 institutes: 21 RENEB members, four candidates and three non-RENEB partners. Results: The duration of a single exercise never exceeded 10 days, while the response from the assisting laboratories never came later than within half a day. During each week of the exercise, around 4500 samples were reported by all service laboratories (SL) to be examined and 54 scenarios were coherently estimated by all laboratories (the standard deviation from the mean of all SL answers for a given scenario category and a set of data was not larger than 3 patient codes). Conclusions: Each participant received training in both the role of a reference laboratory (activating the network) and of a service laboratory (responding to an activation request). The procedures in the case of radiological event were successfully established and tested.

Published
2017
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37. Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans - joint RENEB and EURADOS inter-laboratory comparisons

Author

Ainsbury, Elizabeth, Badie, Christophe, Barnard, Stephen, Manning, Grainne, Moquet, Jayne, Abend, Michael, Antunes, Ana Catarina, Barrios, Lleonard, Bassinet, Celine, Beinke, Christina, Bortolin, Emanuela, Bossin, Lily, Bricknell, Clare, Brzoska, Kamil, Buraczewska, Iwona, Huertas Castano, Carlos, Cemusova, Zina, Christiansson, Maria, Mateos Cordero, Santiago, Coster, Guillaume, Della Monac, Sara, Desangles, Francois, Discher, Michael, Dominguez, Inmaculada, Doucha-Senf, Sven, Eakins, Jon, Fattibene, Paola, Filippi, Silvia, Frenzel, Monika, Georgieva, Dimka, Gregoire, Eric, Guogyte, Kamile, Hadjidekova, Valeria, Hadjiiska, Ljubomira, Hristova, Rositsa, Karakosta, Maria, Kis, Eniko, Kriehuber, Ralf, Lee, Jungil, Lloyd, David, Lumniczky, Katalin, Lyng, Fiona, Macaeva, Ellina, Majewski, Matthaeus, Vanda Martins, S., McKeever, Stephen W. S., Meade, Aidan, Medipally, Dinesh, Meschini, Roberta, M'kacher, Radhia, Gil, Octavia Monteiro, Montero, Alegria, Moreno, Mercedes, Noditi, Mihaela, Oestreicher, Ursula, Oskamp, Dominik, Palitti, Fabrizio, Palma, Valentina, Pantelias, Gabriel, Pateux, Jerome, Patrono, Clarice, Pepe, Gaetano, Port, Matthias, Jesus Prieto, Maria, Quattrini, Maria Cristina, Quintens, Roel, Ricoul, Michelle, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Sholom, Sergey, Sommer, Sylwester, Staynova, Albena, Strunz, Sonja, Terzoudi, Georgia, Testa, Antonella, Trompier, Francois, Valente, Marco, Van Hoey, Olivier, Veronese, Ivan, Wojcik, Andrzej, Woda, Clemens, Ainsbury, Elizabeth, Badie, Christophe, Barnard, Stephen, Manning, Grainne, Moquet, Jayne, Abend, Michael, Antunes, Ana Catarina, Barrios, Lleonard, Bassinet, Celine, Beinke, Christina, Bortolin, Emanuela, Bossin, Lily, Bricknell, Clare, Brzoska, Kamil, Buraczewska, Iwona, Huertas Castano, Carlos, Cemusova, Zina, Christiansson, Maria, Mateos Cordero, Santiago, Coster, Guillaume, Della Monac, Sara, Desangles, Francois, Discher, Michael, Dominguez, Inmaculada, Doucha-Senf, Sven, Eakins, Jon, Fattibene, Paola, Filippi, Silvia, Frenzel, Monika, Georgieva, Dimka, Gregoire, Eric, Guogyte, Kamile, Hadjidekova, Valeria, Hadjiiska, Ljubomira, Hristova, Rositsa, Karakosta, Maria, Kis, Eniko, Kriehuber, Ralf, Lee, Jungil, Lloyd, David, Lumniczky, Katalin, Lyng, Fiona, Macaeva, Ellina, Majewski, Matthaeus, Vanda Martins, S., McKeever, Stephen W. S., Meade, Aidan, Medipally, Dinesh, Meschini, Roberta, M'kacher, Radhia, Gil, Octavia Monteiro, Montero, Alegria, Moreno, Mercedes, Noditi, Mihaela, Oestreicher, Ursula, Oskamp, Dominik, Palitti, Fabrizio, Palma, Valentina, Pantelias, Gabriel, Pateux, Jerome, Patrono, Clarice, Pepe, Gaetano, Port, Matthias, Jesus Prieto, Maria, Quattrini, Maria Cristina, Quintens, Roel, Ricoul, Michelle, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Sholom, Sergey, Sommer, Sylwester, Staynova, Albena, Strunz, Sonja, Terzoudi, Georgia, Testa, Antonella, Trompier, Francois, Valente, Marco, Van Hoey, Olivier, Veronese, Ivan, Wojcik, Andrzej, and Woda, Clemens

Abstract

Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There is a requirement to test and/or validate these candidate techniques and inter-comparison exercises are a well-established method for such validation. Materials and methods: The authors present details of inter-comparisons of four such new methods: dicentric chromosome analysis including telomere and centromere staining; the gene expression assay carried out in whole blood; Raman spectroscopy on blood lymphocytes, and detection of radiation induced thermoluminescent signals in glass screens taken from mobile phones. Results: In general the results show good agreement between the laboratories and methods within the expected levels of uncertainty, and thus demonstrate that there is a lot of potential for each of the candidate techniques. Conclusions: Further work is required before the new methods can be included within the suite of reliable dosimetry methods for use by RENEB partners and others in routine and emergency response scenarios.

Published
2017
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38. Dose assessment intercomparisons within the RENEB network using G(0)-lymphocyte prematurely condensed chromosomes (PCC assay)

Author

Terzoudi, Georgia I., Pantelias, Gabriel, Darroudi, Firouz, Barszczewska, Katarzyna, Buraczewska, Iwona, Depuydt, Julie, Georgieva, Dimka, Hadjidekova, Valeria, Hatzi, Vasiliki I., Karachristou, Ioanna, Karakosta, Maria, Meschini, Roberta, M'Kacher, Radhia, Montoro, Alegria, Palitti, Fabrizio, Pantelias, Antonio, Pepe, Gaetano, Ricoul, Michelle, Sabatier, Laure, Sebastia, Natividad, Sommer, Sylwester, Vral, Anne, Zafiropoulos, Demetre, Wojcik, Andrzej, Terzoudi, Georgia I., Pantelias, Gabriel, Darroudi, Firouz, Barszczewska, Katarzyna, Buraczewska, Iwona, Depuydt, Julie, Georgieva, Dimka, Hadjidekova, Valeria, Hatzi, Vasiliki I., Karachristou, Ioanna, Karakosta, Maria, Meschini, Roberta, M'Kacher, Radhia, Montoro, Alegria, Palitti, Fabrizio, Pantelias, Antonio, Pepe, Gaetano, Ricoul, Michelle, Sabatier, Laure, Sebastia, Natividad, Sommer, Sylwester, Vral, Anne, Zafiropoulos, Demetre, and Wojcik, Andrzej

Abstract

Purpose: Dose assessment intercomparisons within the RENEB network were performed for triage biodosimetry analyzing G(0)-lymphocyte PCC for harmonization, standardization and optimization of the PCC assay. Materials and methods: Comparative analysis among different partners for dose assessment included shipment of PCC-slides and captured images to construct dose-response curves for up to 6 Gy gamma-rays. Accident simulation exercises were performed to assess the suitability of the PCC assay by detecting speed of analysis and minimum number of cells required for categorization of potentially exposed individuals. Results: Calibration data based on Giemsa-stained fragments in excess of 46 PCC were obtained by different partners using galleries of PCC images for each dose-point. Mean values derived from all scores yielded a linear dose-response with approximately 4 excess-fragments/cell/Gy. To unify scoring criteria, exercises were carried out using coded PCC-slides and/or coded irradiated blood samples. Analysis of samples received 24h post-exposure was successfully performed using Giemsa staining (1 excess-fragment/cell/Gy) or centromere/telomere FISH-staining for dicentrics. Conclusions: Dose assessments by RENEB partners using appropriate calibration curves were mostly in good agreement. The PCC assay is quick and reliable for whole- or partial-body triage biodosimetry by scoring excess-fragments or dicentrics in G(0)-lymphocytes. Particularly, analysis of Giemsa-stained excess PCC-fragments is simple, inexpensive and its automation could increase throughput and scoring objectivity of the PCC assay.

Published
2017
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39. RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)

Author

Oestreicher, Ursula, Samaga, Daniel, Ainsbury, Elizabeth, Antunes, Ana Catarina, Baeyens, Ans, Barrios, Leonardo, Beinke, Christina, Beukes, Philip, Blakely, William F., Cucu, Alexandra, De Amicis, Andrea, Depuydt, Julie, De Sanctis, Stefania, Di Giorgio, Marina, Dobos, Katalin, Dominguez, Inmaculada, Pham, Ngoc, Espinoza, Marco E., Flegal, Farrah N., Figel, Markus, Garcia, Omar, Gil, Octavia Monteiro, Gregoire, Eric, Guerrero-Carbajal, C., Guclu, Inci, Hadjidekova, Valeria, Hande, Prakash, Kulka, Ulrike, Lemon, Jennifer, Lindholm, Carita, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Maznyk, Nataliya, Meschini, Roberta, M'kacher, Radia, Montoro, Alegria, Moquet, Jayne, Moreno, Mercedes, Noditi, Mihaela, Pajic, Jelena, Radl, Analia, Ricoul, Michelle, Romm, Horst, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Slabbert, Jacobus, Sommer, Sylwester, Oliveira, Monica Stuck, Subramanian, Uma, Suto, Yumiko, Tran, Que, Testa, Antonella, Terzoudi, Georgia, Vral, Anne, Wilkins, Ruth, Yanti, LusiYanti, Zafiropoulos, Demetre, Wojcik, Andrzej, Oestreicher, Ursula, Samaga, Daniel, Ainsbury, Elizabeth, Antunes, Ana Catarina, Baeyens, Ans, Barrios, Leonardo, Beinke, Christina, Beukes, Philip, Blakely, William F., Cucu, Alexandra, De Amicis, Andrea, Depuydt, Julie, De Sanctis, Stefania, Di Giorgio, Marina, Dobos, Katalin, Dominguez, Inmaculada, Pham, Ngoc, Espinoza, Marco E., Flegal, Farrah N., Figel, Markus, Garcia, Omar, Gil, Octavia Monteiro, Gregoire, Eric, Guerrero-Carbajal, C., Guclu, Inci, Hadjidekova, Valeria, Hande, Prakash, Kulka, Ulrike, Lemon, Jennifer, Lindholm, Carita, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Maznyk, Nataliya, Meschini, Roberta, M'kacher, Radia, Montoro, Alegria, Moquet, Jayne, Moreno, Mercedes, Noditi, Mihaela, Pajic, Jelena, Radl, Analia, Ricoul, Michelle, Romm, Horst, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Slabbert, Jacobus, Sommer, Sylwester, Oliveira, Monica Stuck, Subramanian, Uma, Suto, Yumiko, Tran, Que, Testa, Antonella, Terzoudi, Georgia, Vral, Anne, Wilkins, Ruth, Yanti, LusiYanti, Zafiropoulos, Demetre, and Wojcik, Andrzej

Abstract

Purpose: Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing the European Network of Biodosimetry (RENEB)' to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network. Materials and methods: The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95% confidence intervals were submitted by the participants. Results: The shipment of blood samples to the partners in the European Community (EU) were performed successfully. Outside the EU unacceptable delays occurred. The results of the dose estimation demonstrate a very successful classification of the blood samples in medically relevant groups. In comparison to the 1st exercise the 2nd intercomparison showed an improvement in the accuracy of dose estimations especially for the high dose point. Conclusions: In case of a large-scale radiological incident, the pooling of ressources by networks can enhance the rapid classification of individuals in medically relevant treatment groups based on the DCA. The performance of the RENEB network as a whole has clearly benefited from harmonization processes and specific training activities for the network partners.

Published
2017
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42. Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay)

Author

Terzoudi, Georgia I., primary, Pantelias, Gabriel, additional, Darroudi, Firouz, additional, Barszczewska, Katarzyna, additional, Buraczewska, Iwona, additional, Depuydt, Julie, additional, Georgieva, Dimka, additional, Hadjidekova, Valeria, additional, Hatzi, Vasiliki I., additional, Karachristou, Ioanna, additional, Karakosta, Maria, additional, Meschini, Roberta, additional, M’Kacher, Radhia, additional, Montoro, Alegria, additional, Palitti, Fabrizio, additional, Pantelias, Antonio, additional, Pepe, Gaetano, additional, Ricoul, Michelle, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Vral, Anne, additional, Zafiropoulos, Demetre, additional, and Wojcik, Andrzej, additional

Published
2016
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43. RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)

Author

Oestreicher, Ursula, primary, Samaga, Daniel, additional, Ainsbury, Elizabeth, additional, Antunes, Ana Catarina, additional, Baeyens, Ans, additional, Barrios, Leonardo, additional, Beinke, Christina, additional, Beukes, Philip, additional, Blakely, William F., additional, Cucu, Alexandra, additional, De Amicis, Andrea, additional, Depuydt, Julie, additional, De Sanctis, Stefania, additional, Di Giorgio, Marina, additional, Dobos, Katalin, additional, Dominguez, Inmaculada, additional, Duy, Pham Ngoc, additional, Espinoza, Marco E., additional, Flegal, Farrah N., additional, Figel, Markus, additional, Garcia, Omar, additional, Monteiro Gil, Octávia, additional, Gregoire, Eric, additional, Guerrero-Carbajal, C., additional, Güçlü, İnci, additional, Hadjidekova, Valeria, additional, Hande, Prakash, additional, Kulka, Ulrike, additional, Lemon, Jennifer, additional, Lindholm, Carita, additional, Lista, Florigio, additional, Lumniczky, Katalin, additional, Martinez-Lopez, Wilner, additional, Maznyk, Nataliya, additional, Meschini, Roberta, additional, M’kacher, Radia, additional, Montoro, Alegria, additional, Moquet, Jayne, additional, Moreno, Mercedes, additional, Noditi, Mihaela, additional, Pajic, Jelena, additional, Radl, Analía, additional, Ricoul, Michelle, additional, Romm, Horst, additional, Roy, Laurence, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Slabbert, Jacobus, additional, Sommer, Sylwester, additional, Stuck Oliveira, Monica, additional, Subramanian, Uma, additional, Suto, Yumiko, additional, Que, Tran, additional, Testa, Antonella, additional, Terzoudi, Georgia, additional, Vral, Anne, additional, Wilkins, Ruth, additional, Yanti, LusiYanti, additional, Zafiropoulos, Demetre, additional, and Wojcik, Andrzej, additional

Published
2016
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44. Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation

Author

Trompier, François, primary, Baumann, Marion, additional, Barrios, Lleonard, additional, Gregoire, Eric, additional, Abend, Michael, additional, Ainsbury, Elizabeth, additional, Barnard, Stephen, additional, Barquinero, Joan Francesc, additional, Bautista, Juan Antonio, additional, Brzozowska, Beata, additional, Perez-Calatayud, Jose, additional, De Angelis, Cinzia, additional, Domínguez, Inmaculada, additional, Hadjidekova, Valeria, additional, Kulka, Ulrike, additional, Mateos, Juan Carlos, additional, Meschini, Roberta, additional, Monteiro Gil, Octávia, additional, Moquet, Jayne, additional, Oestreicher, Ursula, additional, Montoro Pastor, Alegria, additional, Quintens, Roel, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Stoyanov, Orlin, additional, Thierens, Hubert, additional, Terzoudi, Georgia, additional, Villaescusa, Juan Ignacio, additional, Vral, Anne, additional, Wojcik, Andrzej, additional, Zafiropoulos, Demetre, additional, and Roy, Laurence, additional

Published
2016
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45. RENEB – Running the European Network of biological dosimetry and physical retrospective dosimetry

Author

Kulka, Ulrike, primary, Abend, Michael, additional, Ainsbury, Elizabeth, additional, Badie, Christophe, additional, Barquinero, Joan Francesc, additional, Barrios, Lleonard, additional, Beinke, Christina, additional, Bortolin, Emanuela, additional, Cucu, Alexandra, additional, De Amicis, Andrea, additional, Domínguez, Inmaculada, additional, Fattibene, Paola, additional, Frøvig,, Anne Marie, additional, Gregoire, Eric, additional, Guogyte, Kamile, additional, Hadjidekova, Valeria, additional, Jaworska, Alicja, additional, Kriehuber, Ralf, additional, Lindholm, Carita, additional, Lloyd, David, additional, Lumniczky, Katalin, additional, Lyng, Fiona, additional, Meschini, Roberta, additional, Mörtl, Simone, additional, Della Monaca, Sara, additional, Monteiro Gil, Octávia, additional, Montoro, Alegria, additional, Moquet, Jayne, additional, Moreno, Mercedes, additional, Oestreicher, Ursula, additional, Palitti, Fabrizio, additional, Pantelias, Gabriel, additional, Patrono, Clarice, additional, Piqueret-Stephan, Laure, additional, Port, Matthias, additional, Prieto, María Jesus, additional, Quintens, Roel, additional, Ricoul, Michelle, additional, Romm, Horst, additional, Roy, Laurence, additional, Sáfrány, Géza, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Terzoudi, Georgia, additional, Testa, Antonella, additional, Thierens, Hubert, additional, Turai, Istvan, additional, Trompier, François, additional, Valente, Marco, additional, Vaz, Pedro, additional, Voisin, Philippe, additional, Vral, Anne, additional, Woda, Clemens, additional, Zafiropoulos, Demetre, additional, and Wojcik, Andrzej, additional

Published
2016
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46. Capabilities of the RENEB network for research and large scale radiological and nuclear emergency situations

Author

Monteiro Gil, Octávia, primary, Vaz, Pedro, additional, Romm, Horst, additional, De Angelis, Cinzia, additional, Antunes, Ana Catarina, additional, Barquinero, Joan-Francesc, additional, Beinke, Christina, additional, Bortolin, Emanuela, additional, Burbidge, Christopher Ian, additional, Cucu, Alexandra, additional, Della Monaca, Sara, additional, Domene, Mercedes Moreno, additional, Fattibene, Paola, additional, Gregoire, Eric, additional, Hadjidekova, Valeria, additional, Kulka, Ulrike, additional, Lindholm, Carita, additional, Meschini, Roberta, additional, M’Kacher, Radhia, additional, Moquet, Jayne, additional, Oestreicher, Ursula, additional, Palitti, Fabrizio, additional, Pantelias, Gabriel, additional, Montoro Pastor, Alegria, additional, Popescu, Irina-Anca, additional, Quattrini, Maria Cristina, additional, Ricoul, Michelle, additional, Rothkamm, Kai, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Terzoudi, Georgia, additional, Testa, Antonella, additional, Trompier, François, additional, and Vral, Anne, additional

Published
2016
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47. RENEB accident simulation exercise

Author

Brzozowska, Beata, primary, Ainsbury, Elizabeth, additional, Baert, Annelot, additional, Beaton-Green, Lindsay, additional, Barrios, Leonardo, additional, Barquinero, Joan Francesc, additional, Bassinet, Celine, additional, Beinke, Christina, additional, Benedek, Anett, additional, Beukes, Philip, additional, Bortolin, Emanuela, additional, Buraczewska, Iwona, additional, Burbidge, Christopher, additional, De Amicis, Andrea, additional, De Angelis, Cinzia, additional, Della Monaca, Sara, additional, Depuydt, Julie, additional, De Sanctis, Stefania, additional, Dobos, Katalin, additional, Domene, Mercedes Moreno, additional, Domínguez, Inmaculada, additional, Facco, Eva, additional, Fattibene, Paola, additional, Frenzel, Monika, additional, Monteiro Gil, Octávia, additional, Gonon, Géraldine, additional, Gregoire, Eric, additional, Gruel, Gaëtan, additional, Hadjidekova, Valeria, additional, Hatzi, Vasiliki I., additional, Hristova, Rositsa, additional, Jaworska, Alicja, additional, Kis, Enikő, additional, Kowalska, Maria, additional, Kulka, Ulrike, additional, Lista, Florigio, additional, Lumniczky, Katalin, additional, Martínez-López, Wilner, additional, Meschini, Roberta, additional, Moertl, Simone, additional, Moquet, Jayne, additional, Noditi, Mihaela, additional, Oestreicher, Ursula, additional, Orta Vázquez, Manuel Luis, additional, Palma, Valentina, additional, Pantelias, Gabriel, additional, Montoro Pastor, Alegria, additional, Patrono, Clarice, additional, Piqueret-Stephan, Laure, additional, Quattrini, Maria Cristina, additional, Regalbuto, Elisa, additional, Ricoul, Michelle, additional, Roch-Lefevre, Sandrine, additional, Roy, Laurence, additional, Sabatier, Laure, additional, Sarchiapone, Lucia, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Sun, Mingzhu, additional, Suto, Yumiko, additional, Terzoudi, Georgia, additional, Trompier, Francois, additional, Vral, Anne, additional, Wilkins, Ruth, additional, Zafiropoulos, Demetre, additional, Wieser, Albrecht, additional, Woda, Clemens, additional, and Wojcik, Andrzej, additional

Published
2016
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49. Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Author

Ainsbury, Elizabeth, primary, Badie, Christophe, additional, Barnard, Stephen, additional, Manning, Grainne, additional, Moquet, Jayne, additional, Abend, Michael, additional, Antunes, Ana Catarina, additional, Barrios, Lleonard, additional, Bassinet, Celine, additional, Beinke, Christina, additional, Bortolin, Emanuela, additional, Bossin, Lily, additional, Bricknell, Clare, additional, Brzoska, Kamil, additional, Buraczewska, Iwona, additional, Castaño, Carlos Huertas, additional, Čemusová, Zina, additional, Christiansson, Maria, additional, Cordero, Santiago Mateos, additional, Cosler, Guillaume, additional, Monaca, Sara Della, additional, Desangles, François, additional, Discher, Michael, additional, Dominguez, Inmaculada, additional, Doucha-Senf, Sven, additional, Eakins, Jon, additional, Fattibene, Paola, additional, Filippi, Silvia, additional, Frenzel, Monika, additional, Georgieva, Dimka, additional, Gregoire, Eric, additional, Guogyte, Kamile, additional, Hadjidekova, Valeria, additional, Hadjiiska, Ljubomira, additional, Hristova, Rositsa, additional, Karakosta, Maria, additional, Kis, Enikő, additional, Kriehuber, Ralf, additional, Lee, Jungil, additional, Lloyd, David, additional, Lumniczky, Katalin, additional, Lyng, Fiona, additional, Macaeva, Ellina, additional, Majewski, Matthaeus, additional, Vanda Martins, S., additional, McKeever, Stephen W.S., additional, Meade, Aidan, additional, Medipally, Dinesh, additional, Meschini, Roberta, additional, M’kacher, Radhia, additional, Gil, Octávia Monteiro, additional, Montero, Alegria, additional, Moreno, Mercedes, additional, Noditi, Mihaela, additional, Oestreicher, Ursula, additional, Oskamp, Dominik, additional, Palitti, Fabrizio, additional, Palma, Valentina, additional, Pantelias, Gabriel, additional, Pateux, Jerome, additional, Patrono, Clarice, additional, Pepe, Gaetano, additional, Port, Matthias, additional, Prieto, María Jesús, additional, Quattrini, Maria Cristina, additional, Quintens, Roel, additional, Ricoul, Michelle, additional, Roy, Laurence, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Sholom, Sergey, additional, Sommer, Sylwester, additional, Staynova, Albena, additional, Strunz, Sonja, additional, Terzoudi, Georgia, additional, Testa, Antonella, additional, Trompier, Francois, additional, Valente, Marco, additional, Hoey, Olivier Van, additional, Veronese, Ivan, additional, Wojcik, Andrzej, additional, and Woda, Clemens, additional

Published
2016
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50. Optimized conditions for the isolation of mesophyll protoplasts along the growing season from <italic>Arbutus unedo</italic> and their use in single cell gel electrophoresis.

Author

Choury, Zineb, Meschini, Roberta, Dell’Orso, Ambra, Fardusi, Most Jannatul, Mugnozza, Giuseppe Scarascia, and Kuzminsky, Elena

Abstract

Global warming is affecting Mediterranean ecosystems, in which Arbutus unedo represents an important species. Mesophyll protoplasts are convenient material to study plant DNA integrity, nevertheless their release from mature plants along the year has not been reported in sclerophyll species. In this respect, in the present study the chance to isolate protoplasts from leaf tissues of A. unedo in different seasons was investigated. The digestion was obtained using Macerozyme R10 (1%) and Cellulase Onozuka RS (2%), with 0.6 M of mannitol, incubated for 4 h at 25 °C in the dark. In spring, soft leaves of different ages were studied to identify the most suitable material and protoplast yields were significantly influenced, with the still expanding 4th leaf characterized by the highest amount. Protoplast release decreased during the growing season when leaves become partially hardened, while their obtainment was quite impossible in summer and winter, due to leaf hardening. In November, an increase of incubation time (16 h) and of Cellulase RS (4%) or mannitol concentration (0.8 M) was needed with chilled leaves from the field; conversely, a good source for protoplasts were the green soft leaves coming from detached and pruned branches placed in controlled environment at spring temperatures. The employment of polyvinylpyrrolidone-40 during isolation procedure is discussed. The isolated protoplasts were employed to assess the DNA integrity, by using single cell gel electrophoresis (SCGE). The X-irradiated nucleoids were significantly damaged confirming that the present approach could be used in the selection of elite material of A. unedo devoted to fruit plantation. [ABSTRACT FROM AUTHOR]

Published
2018
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