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Background: Food safety and food-borne infections are major subjects with global interest. Dairy products are considered as important sources for these infections. Aim: The present study was conducted to identify the occurrence and to genotype isolates of Staphylococcus spp. recovered from milk samples in Al-Diwaniyah City, Iraq. Methods: The current study included the collection of 50 milk samples purchased from local stores in the current city. These samples were subjected to bacterial cultivation and biochemical tests. Later, the growth was used to extract the genomic DNA that was exposed to PCR and partial gene sequencing that both targeted the 16S rRNA gene at a specific genetic piece. Results: The PCR results demonstrated the amplification of the genetic fragment of five genetic clusters for each of S. aureus (SAD11, SAD12, SAD13, SAD14 and SAD15), S. epidermidis (SED1, SED2, SED3, SED4 and SED5), and S.intermedius (SID1, SID2, SID3, SID4 and SID5). The PCR products were sent out to sequencing and reported that the current isolates were similar in their genetic content with global isolates at 95.34% to 97.59%, 96.21% to 97.57%, and 96.09% to 97.88%, respectively, of identity. Conclusion: The present findings show highly genetic variations among isolates of S. aureus, S. epidermidis, and S.intermedius recovered from milk samples, and these genotypes are found in different infection setting related to humans and animals, which may pose high risks to the humans and animals. [Open Vet J 2024; 14(7.000): 1689-1700]

Background: The Brief Illness Perception Questionnaire (B-IPQ) is one of the most widely used measures of emotional and cognitive representations of illness. The B-IPQ has been translated and adapted into many languages. However, the scale has not been translated into Iraqi Arabic in patients with type II diabetes. The main purpose of the present study was to investigate psychometric properties of the B-IPQ among Iraqi patients with type II diabetes. Methods: In this quantitative study, the Arabic Version of the B-IPQ was given to 192 Iraqi patients with type II diabetes. The participants were randomly selected from 5 hospitals and a diabetes society in Baghdad, Iraq. The participants included individuals diagnosed at least 1 year before this research. Using WINSTEPS computer program, the Rating Scale Model ‎(RSM) was employed as a polytomous extension of the Rasch model (RM) to evaluate the scale in terms of unidimensionality, local independence, item statistics, and rating structures. Results: The results indicated that the values of infit and oufit mean square (MNSQ) are within the ideal range of 0.60 and 1.40, suggesting that the items of the scale fit to the RM. The data was found to be unidimensional because the first factor explains 5.5% of the unexplained variance with an eigenvalue of 1.5 (< 2). The results also showed that items are locally independent, and both persons and items have high Rasch separation reliability indices. More importantly, the response options or categories of the scale work optimally because with the increase in category values, observed averages also increased. Conclusion: The overall findings showed that the Arabic version of the B-IPQ is a valid and reliable instrument and can be employed to assess illness perceptions among Iraqi patients with type II diabetes.

Background and Aim: Despite the importance of the global emergence of Vibrio parahaemolyticus infections worldwide, there has been scanty information on its occurrence in Malaysian seawaters and fish. This study aimed to determine the occurrence of V. parahaemolyticus isolates using polymerase chain reaction targeted at toxin operon gene, thermostable direct hemolysin (tdh), and tdh-related hemolysin genes and to determine antibiotic resistance pattern, genes, and plasmid profile of V. parahaemolyticus from Malaysian seawaters and fish. Materials and Methods: Samples were collected from four recreational beaches in Malaysia (Port Klang; Bachok; Port Dickson; and Mersing). Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and chromogenic Vibrio agar were used for isolation and identification. Colonies with yellow color on TCBS and green color on chromogenic vibrio (CV) agar were considered to be V. parahaemolyticus and they were subjected to biochemical tests. All V. parahaemolyticus isolates were further subjected to identification using seven specific gene markers. Results: Seventy-three Vibrio isolates were recovered. Only one gene tdh> from seawater isolates of Vibrio has high virulence gene percentage (95.23%). Two genes alkaline serine protease (asp) and (tdh) had high percentage of virulence (83.87% and 80.64%, respectively) from fish. Comparatively, fish isolates have a higher virulence percentage compared to seawater isolates. Only gene streptomycin resistance B (strB) from seawater had 100% of the resistance genes. All isolates were multi-antibiotic resistant. Seventeen antibiotic resistance patterns were observed. The isolates had plasmids of varying sizes ranging from 2.7 kb to 42.4 kb. Dendrogram based on antibiotic resistance patterns of V. parahaemolyticus isolates discriminated the isolates into three clusters. Conclusion: This study demonstrated the occurrence of pathogenic, multi-antibiotic-resistant V. parahaemolyticus strains in Malaysian coastal waters and fish, and this could constitute potential public health risks.