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This study was conducted on Klasztorne Małe Lake located in the city of Kartuzy. For about 100 years, the lake served as a receiver of domestic, industrial, and rain sewage. The lake was completely degraded. In addition, excessive sewage inflow caused the accumulation of dense pollution in the area of the lake's deepest part, which caused the formation of a monimolimnion layer. The bradymictic KML became a meromictic lake. After cutting off the inflow of pollutants, the lake was restored by the P inactivation method using coagulants PIX 111 and PAX 18. Before the restoration process of this lake, concentrations of nutrients in the bottom water layers achieved very high values of TN (total N) 65 mg/L and TP (total P) 30 mg/L. An average concentration of chlorophyll was 65 µg/L and visibility did not exceed 1 m. The method used brought very good results. In the entire volume of water, over 90% reduction in TP and a several-fold decrease in the content of TN were noted. The withdrawal of mineral P from the water limited the production processes in the lake, which was reflected in the reduction in chlorophyll amounts (19 µg/L) and the increase in water visibility (2 m). In addition, oxygen conditions in the lake improved. The application of coagulants caused a decrease in the density of the monimolimnion water, which was indicated by a significant decrease in water conductivity. However, the restoration method did not improve the water dynamics. [ABSTRACT FROM AUTHOR]

There are numerous deep coal mines in the Upper Silesian Coal Basin. Ensuring their proper exploitation requires constant drainage of the rock mass and the transfer of mine waters to rivers. Several technical solutions are used to prevent the adverse effects of saltwater discharge on the river ecosystem. One such solution is adapting the post-mining reservoirs into mine water settling basins. This article characterises two such facilities—the “Gliniok” and “Hubertus I” reservoirs. The physicochemical properties of their waters were analysed both when they served as settling basins and after their decommissioning. During their exploitation, the waters of the settling basins showed high salinity (>10 g/L). It was revealed that these basins turned into freshwater reservoirs very quickly after decommissioning. A sudden decrease in the electrolytic conductivity and the concentration of main cations and anions in the water was observed. The mixing processes also changed. The reservoirs were transformed from meromictic to polymictic. The processes that led to them turning into freshwater basins differed in the studied settling basins. The transformation of the Gliniok settling basin into a freshwater reservoir was a unique process, draining brines into the rock mass through cracks and crevices. The formation of cracks and crevices was a consequence of high-energy mining tremors. It is the first known case of this type in the world.

Introduction: Bioconvection, a phenomenon characterized by the collective upward swimming of motile microorganisms, has mainly been investigated within controlled laboratory settings, leaving a knowledge gap regarding its ecological implications in natural aquatic environments. This study aims to address this question by investigating the influence of bioconvection on the eco-physiology of the anoxygenic phototrophic sulfur bacteria community of meromictic Lake Cadagno. Methods: Here we comprehensively explore its effects by comparing the physicochemical profiles of the water column and the physiological traits of the main populations of the bacterial layer (BL). The search for eco-physiological effects of bioconvection involved a comparative analysis between two time points during the warm season, one featuring bioconvection (July) and the other without it (September). Results: A prominent distinction in the physicochemical profiles of the water column centers on light availability, which is significantly higher in July. This minimum threshold of light intensity is essential for sustaining the physiological CO2 fixation activity of Chromatium okenii, the microorganism responsible for bioconvection. Furthermore, the turbulence generated by bioconvection redistributes sulfides to the upper region of the BL and displaces other microorganisms from their optimal ecological niches. Conclusion: The findings underscore the influence of bioconvection on the physiology of C. okenii and demonstrate its functional role in improving its metabolic advantage over coexisting phototrophic sulfur bacteria. However, additional research is necessary to confirm these results and to unravel the multiscale processes activated by C. okenii’s motility mechanisms. [ABSTRACT FROM AUTHOR]

IntroductionBioconvection, a phenomenon characterized by the collective upward swimming of motile microorganisms, has mainly been investigated within controlled laboratory settings, leaving a knowledge gap regarding its ecological implications in natural aquatic environments. This study aims to address this question by investigating the influence of bioconvection on the eco-physiology of the anoxygenic phototrophic sulfur bacteria community of meromictic Lake Cadagno.MethodsHere we comprehensively explore its effects by comparing the physicochemical profiles of the water column and the physiological traits of the main populations of the bacterial layer (BL). The search for eco-physiological effects of bioconvection involved a comparative analysis between two time points during the warm season, one featuring bioconvection (July) and the other without it (September).ResultsA prominent distinction in the physicochemical profiles of the water column centers on light availability, which is significantly higher in July. This minimum threshold of light intensity is essential for sustaining the physiological CO2 fixation activity of Chromatium okenii, the microorganism responsible for bioconvection. Furthermore, the turbulence generated by bioconvection redistributes sulfides to the upper region of the BL and displaces other microorganisms from their optimal ecological niches.ConclusionThe findings underscore the influence of bioconvection on the physiology of C. okenii and demonstrate its functional role in improving its metabolic advantage over coexisting phototrophic sulfur bacteria. However, additional research is necessary to confirm these results and to unravel the multiscale processes activated by C. okenii’s motility mechanisms.

Lake Kislo-Sladkoe is a stratified water body partly isolated from the White Sea. Perennial meromixis in the lake irregularly alternates with mixing events. Taking into account that the protists of Arctic coastal stratified water bodies are understudied, we evaluated for the first time the vertical structure, species richness, and diversity of protists assigned to different taxonomic groups in Lake Kislo-Sladkoe using light, luminescent, and scanning electron microscopy. To test the research hypothesis that a mixing event affects the vertical stratification and species composition of protists in a stratified lake, we compared the protist communities of Lake Kislo-Sladkoe in two extremely different states: strong meromixis vs. full vertical mixing. A total of 97 morphologically distinct phototrophic, heterotrophic, and mixotrophic protists were revealed with the most diverse supertaxa SAR (59), Obazoa (9), and Excavates (14). The hidden diversity of protists (43 species) was a bit less than the active diversity (54 species). A taxonomic list and micrographs of cells for the observed protists are provided. The majority of species revealed are cosmopolitan or widespread in the northern sea waters. The vertical patterns of protist communities were absolutely different in 2018 and 2021. In July 2018, clearly distinct protist communities inhabited different layers of the lake. Bloom of cryptophyte Rhodomonas cf. baltica was detected in chemocline, whereas the maximum density of its grazers was observed in adjacent layers, mainly dinoflagellates Gymnodinium sp. and Scrippsiella trochoidea, as well as a ciliate Prorodon sp. In 2021 due to the recent mixing of lake and seawater, there were no distinct communities in the water column except the superficial 0–1 m layer of fresh water. [ABSTRACT FROM AUTHOR]

In Solotvino, in southwestern Ukraine (Transcarpathia), there is a large group of anthropogenic water reservoirs. Most of these developed in sinkholes formed by the flooding of salt mines and the activation of anthropogenic and karst processes. One reservoir, Solotvino No. 7, was the subject of detailed limnological (hydrographic and hydrochemical) studies. The reservoir has an area of 8493 m2, a maximum depth of 20.5 m, and holds Cl−–Na+ brines. The water in the near-surface layer is hyposaline (3–20 g/L), but periodically becomes mesosaline (20–50 g/L). Hypersaline waters with mineralization > 250 g/L are found below 3 m. The reservoir has three persistent distinct mixolimnion layers that clearly indicate their meromictic type: the surface layer, a chemocline (where the water chemistry changes), and a lower monimolimnion layer. The thermal properties of the reservoir deserve special attention. The water is heated during all seasons at the boundary between the chemocline and monimolimnion; the water temperature is 32 °C in winter and 54 °C in summer, despite the lack of volcanism. The water is heated by a physical phenomenon in the layer where the water density increases, which is a heliothermal process. Also noteworthy is that throughout the year, the oxygen profiles are positive and heterograde, with the water being up to 380% oxygen saturated. [ABSTRACT FROM AUTHOR]

The article presents data on the composition and abundance of algal flora in the coastal stratified Kislo-Sladkoe Lake (the Karelian coast of the White Sea) after its flushing with seawater in autumn. The seasonal dynamics and vertical structure of the phytoplankton were monitored from March 17 to October 3, 2019. We also studied ice flora on March 17 and April 1. The largest carbon biomass of ice algae (0.79 g C/m3) dominated by Nitzschia frigida was recorded in April. The integrated biomass of phytoplankton in the water column varied from 0.01 g C/m2 in March to 5.83 g C/m2 during spring bloom in late May (when it was dominated by Chaetoceros invisibilis) and remained at the level of 0.07 to 0.29 g C/m2 until October; heterotrophic forms replaced autotrophic algae during this period. A regression analysis has shown that the structure of the phytoplankton was influenced by length of daylight, water temperature, dissolved oxygen level, depth, pH, salinity, and illuminance. [ABSTRACT FROM AUTHOR]

McGuire HE, Dunnington DW, Loder AL, Spooner IS, Mallory ML, McLellan NR, Su, C-C. 2021. Evaluating the multidecadal response of historic seawater incursion events and salinity-induced meromixis at Laytons Lake, Nova Scotia, Canada. Lake Reserv Manage. 37:378–390. Long-term monitoring data from freshwater lakes can be challenging to retrieve yet are valuable for assessing lakes at risk of saltwater intrusion. We demonstrate the use of an applied paleolimnological method to identify the response of a freshwater lake to seawater incursion and salinity-induced meromixis by examining the geochemistry of lake sediments deposited over the past century. Laytons Lake is located 1.5 km from the Bay of Fundy coastline, and experienced documented seawater incursion events in 1944 and 1949 leading to a period of extended meromixis (>40 yr). Our limnological measurements indicate that meromixis no longer exists at Laytons Lake, and hypolimnetic phosphorus (P) concentrations and conductivity values have declined since 1982. Meromictic conditions, the establishment of the chemocline, and high sedimentation rates collectively preserve a geochemical record of seawater incursion, which was identified as a short-duration, positive chloride (Cl-) excursion followed by the precipitation of iron sulfide (FeS2). A decline in Cl- concentration after the incursion events in the paleorecord may represent the deterioration of the chemocline, and the reestablishment of thermal stratification and mixing. We suspect that seawater incursions can have a multidecadal impact on aquatic systems, and that bulk geochemical analysis of paleolimnological records is a useful, low-cost tool that can provide a long-term perspective on the limnological consequences of such events. These long-term data can inform the assessment of aquatic systems at risk of saltwater incursion due to sea-level rise. [ABSTRACT FROM AUTHOR]

Lake Kislo-Sladkoe is a stratified water body partly isolated from the White Sea. Perennial meromixis in the lake irregularly alternates with mixing events. Taking into account that the protists of Arctic coastal stratified water bodies are understudied, we evaluated for the first time the vertical structure, species richness, and diversity of protists assigned to different taxonomic groups in Lake Kislo-Sladkoe using light, luminescent, and scanning electron microscopy. To test the research hypothesis that a mixing event affects the vertical stratification and species composition of protists in a stratified lake, we compared the protist communities of Lake Kislo-Sladkoe in two extremely different states: strong meromixis vs. full vertical mixing. A total of 97 morphologically distinct phototrophic, heterotrophic, and mixotrophic protists were revealed with the most diverse supertaxa SAR (59), Obazoa (9), and Excavates (14). The hidden diversity of protists (43 species) was a bit less than the active diversity (54 species). A taxonomic list and micrographs of cells for the observed protists are provided. The majority of species revealed are cosmopolitan or widespread in the northern sea waters. The vertical patterns of protist communities were absolutely different in 2018 and 2021. In July 2018, clearly distinct protist communities inhabited different layers of the lake. Bloom of cryptophyte Rhodomonas cf. baltica was detected in chemocline, whereas the maximum density of its grazers was observed in adjacent layers, mainly dinoflagellates Gymnodinium sp. and Scrippsiella trochoidea, as well as a ciliate Prorodon sp. In 2021 due to the recent mixing of lake and seawater, there were no distinct communities in the water column except the superficial 0–1 m layer of fresh water.

Climate warming is causing changes in the physics of deep lakes, such as longer summer stratification, increased water column stability, reduced ice cover, and a shallower depth of winter overturns. An ultimate consequence of warming would be a transition to a different mixing regime. Here we investigate the role of physical, chemical, and biological feedback mechanisms that unfold during a shift in mixing regime, and whether these feedbacks could prompt and stabilise the new regime. Although climate, interannual temperature variation, and lake morphometry are the main determinants of a mixing regime, when climate change causes shifts in mixing regime, internal feedback mechanisms may gain in importance and modify lake ecosystem functioning.We review the role of these feedbacks in three mixing regime shifts: from polymictic to seasonally stratified, from dimictic to monomictic, and from holomictic to oligomictic or meromictic.Polymictic lakes of intermediate depth (c. 3–10 m mean depth) could experience seasonal stratification if a stratification event triggers phytoplankton blooms or dissolved organic matter release, reducing transparency and therefore further heating the surface layer. However, this feedback is only likely to have influence in small and clear lakes, it would be easily disturbed by weather conditions, and the resulting stratified state does not remain stable in the long term, as stratification is lost in winter.The ice‐albedo feedback might cause an accelerated shift from ice‐covered (dimictic) to ice‐free (monomictic) winters in sufficiently deep (mean depth 50 m or more) lakes, where temperature memory is carried over from one winter to the next. Nevertheless, there is an ongoing debate into whether this process can persist during natural weather variations and overcome self‐stabilising mechanisms such as thermal insulation by snow. The majority of studies suggest that a gradual transition from dimictic to monomictic is more likely than an abrupt transition.A shift from a holomictic to a meromictic regime can occur if anoxia is triggered by incomplete mixing and an increase in deep‐water density—through the accumulation of solutes—exceeds a density decrease by hypolimnetic warming. A shift to meromixis would strongly alter the biology of a lake and might be difficult to reverse. If solutes accumulate only minimally in the hypolimnion, an oligomictic regime is formed, in which years with complete and incomplete mixing alternate.Understanding the importance of feedback mechanisms and the role of biogeochemistry when lakes shift in mixing regime could lead to a better understanding of how climate change affects lake ecosystems. [ABSTRACT FROM AUTHOR]

Mixing dynamics in the bottom boundary layer (BBL) of lakes is of primary importance for mediating mass and heat fluxes across the upper sediment. In lakes with depth of several hundred meters, the BBL mixing is often suggested to be low; however, quantitative information from these depths is extremely rare. We assessed the mixing conditions in the BBL of Lake Iseo, a 256 m deep, meromictic Italian lake, where anoxia and accumulation of phosphorus is a major issue. High-resolution temperature and currents measurements demonstrated regular development of turbulence at 220 m depth, with dissipation rates of the turbulent kinetic energy up to 10−7 W kg−1, characteristic of a shear boundary layer. Analysis of temperature and oxygen dynamics revealed a direct link between the turbulence intensification in the BBL and the passage of a 90-h period basin-scale internal wave. This slow oscillatory motion was attributed to lake-wide internal waves of second vertical mode, whose effect in the upper part of the water column was much less profound. The wave passages were able to increase hypolimnetic velocities above 1 cm s−1, to produce thermal instability across the BBL and to significantly enhance turbulent mixing in the deepest waters. During periods of high velocity bursts, the chemical stratification in the BBL was effectively eroded while direct sediment resuspension was unlikely. The new results reveal the turbulent character of the bottom boundary mixing in deep lakes, highlighting the direct link to wind-driven motions and important effects on the water–sediment exchange of solutes. [ABSTRACT FROM AUTHOR]

Global spread of hypoxia and altered mixing regimes in freshwater systems is a growing major environmental concern. Climate change and human impact are expected to increasingly deteriorate aquatic ecosystems. The study of processes and drivers of such changes in the past provides a great asset for prevention and remediation in the future. We used a multi-proxy approach combining high-resolution Hyperspectral Imaging (HSI) pigment data, with specific HPLC chlorophylls and carotenoids to examine Holocene trophic state changes and anoxia evolution in meromictic Lake Jaczno, NE Poland. A redundancy analysis RDA including pollen-inferred vegetation cover, temperature and human impacts provides insight into specific conditions and drivers of changing trophic and redox states in the lake. Anoxic and sulfidic conditions established in Lake Jaczno after initial basin infilling 9500 years ago. Until 6700 cal BP, lake trophy was relatively low, water turbidity was high, and green sulfur bacteria (GSB) were abundant within the phototrophic community, suggesting a deep oxic-anoxic boundary and weak stratification. The period between 6700-500 cal BP is characterized by constantly increasing lake production and a gradual shift from GSB to purple sulfur bacteria (PSB), suggesting a shallower oxic-anoxic boundary and pronounced stratification. Yet, the presence of spheroidene and speroidenone in the sediments indicates intermittent anoxia. After 500 cal BP, increasing human impact, deforestation and intensive agriculture promoted lake eutrophication, with a shift to PSB dominance and establishment of permanent anoxia and meromixis. Our study unambiguously documents the legacy of human impact on processes determining eutrophication and anoxia. [ABSTRACT FROM AUTHOR]

Long-term measurements show that the sedimentation dynamics of okenone (a carotenoid of purple sulfur bacteria) reflects the abundance of purple sulfur bacteria, the hydrogen sulfide content, and the stratification stability in the closed saline meromictic Shira Lake (Southern Siberia, Russia). The profile of fossil okenone in bottom sediments has been compared with the data on the water surface level in the lake over the last 100 years. The peaks of okenone correspond to lake transgressions, and the drops in the okenone content coincide with periods of stable or decreasing water level. Our observations show that fossil okenone may be used as a qualitative proxy of meromictic conditions and as a quantitative proxy of the water level dynamics in closed stratified lakes. Therefore, okenone can be used for qualitative reconstructions of the water balance and climate humidity in arid zones. [ABSTRACT FROM AUTHOR]

Estuarine Pettaquamscutt River is a unique habitat 10 km in length with physical and biogeochemical characteristics analogous to a miniature fjord. Its meromictic upper basins are characterized by a permanent oxic–anoxic vertical gradient in which a well-oxygenated upper layer overlies a deeper, anoxic reservoir, with persistent blooms of phototrophic anoxygenic bacteria (Chromatium, Chlorobium) at the oxic–anoxic transition layer. A diverse assemblage of nanoplanktonic, centric diatoms (Cyclotella caspia, Thalassiosira pseudonana, cf. Cyclotella cryptica) dominated the seasonal phytoplankton cycle in the aerobic layer, similar to comparable meromictic habitats elsewhere. This assemblage of nano-centric diatoms appears to be trait-separated from other species clusters and is potentially useful as a functional group flora with ecophysiology diagnostic of marine estuarine rivers and meromictic habitat niche structure. The most conspicuous phytoplankton feature, however, was the year-round occurrence of the photoautotrophic euglenid Euglenaformis (Euglena) proxima, restricted in its upper water column distribution to the O2–H2S boundary layer where it formed a consortium with the photosynthetic green and purple sulfur bacteria community. The association of E. proxima with meromixis, H2S, and phototrophic anoxygenic bacteria is similar to that reported previously in a brackish Norwegian oyster poll and a brackish loch in Scotland. [ABSTRACT FROM AUTHOR]

In deep lakes, many investigations highlighted the existence of exclusive groups of bacteria adapted to deep oxygenated and hypoxic and anoxic hypolimnia. Nevertheless, the extent of bacterial strain diversity has been much less scrutinized. This aspect is essential for an unbiased estimation of genetic variation, biodiversity, and population structure, which are essential for studying important research questions such as biogeographical patterns, temporal and spatial variability and the environmental factors affecting this variability. This study investigated the bacterioplankton community in the epilimnetic layers and in the oxygenated and hypoxic/anoxic hypolimnia of five large and deep lakes located at the southern border of the Alps using high throughput sequencing (HTS) analyses (16S rDNA) and identification of amplicon sequence variants (ASVs) resolving reads differing by as little as one nucleotide. The study sites, which included two oligomictic (Garda and Como) and three meromictic lakes (Iseo, Lugano, and Idro) with maximum depths spanning from 124 to 410 m, were chosen among large lakes to represent an oxic-hypoxic gradient. The analyses showed the existence of several unique ASVs in the three layers of the five lakes. In the case of cyanobacteria, this confirmed previous analyses made at the level of strains or based on oligotyping methods. As expected, the communities in the hypoxic/anoxic monimolimnia showed a strong differentiation from the oxygenated layer, with the exclusive presence in single lakes of several unique ASVs. In the meromictic lakes, results supported the hypothesis that the formation of isolated monimolimnia sustained the development of highly diversified bacterial communities through ecological selection, leading to the establishment of distinctive biodiversity zones. The genera identified in these layers are well-known to activate a wide range of redox reactions at low O2 conditions. As inferred from 16S rDNA data, the highly diversified and coupled processes sustained by the monimolimnetic microbiota are essential ecosystem services that enhance mineralization of organic matter and formation of reduced compounds, and also abatement of undesirable greenhouse gasses.

The microbial ecosystem of the meromictic Lake Cadagno (Ticino, Swiss Alps) has been studied intensively in order to understand structure and functioning of the anoxygenic phototrophic sulfur bacteria community living in the chemocline. It has been found that the purple sulfur bacterium “Thiodictyon syntrophicum” strain Cad16T, belonging to the Chromatiaceae, fixes around 26% of all bulk inorganic carbon in the chemocline, both during day and night. With this study, we elucidated for the first time the mode of carbon fixation of str. Cad16T under micro-oxic conditions with a combination of long-term monitoring of key physicochemical parameters with CTD, 14C-incorporation experiments and quantitative proteomics using in-situ dialysis bag incubations of str. Cad16T cultures. Regular vertical CTD profiling during the study period in summer 2017 revealed that the chemocline sank from 12 to 14 m which was accompanied by a bloom of cyanobacteria and the subsequent oxygenation of the deeper water column. Sampling was performed both day and night. CO2 assimilation rates were higher during the light period compared to those in the dark, both in the chemocline population and in the incubated cultures. The relative change in the proteome between day and night (663 quantified proteins) comprised only 1% of all proteins encoded in str. Cad16T. Oxidative respiration pathways were upregulated at light, whereas stress-related mechanisms prevailed during the night. These results indicate that low light availability and the co-occurring oxygenation of the chemocline induced mixotrophic growth in str. Cad16T. Our study thereby helps to further understand the consequences micro-oxic conditions for phototrophic sulfur oxidizing bacteria. The complete proteome data have been deposited to the ProteomeXchange database with identifier PXD010641.

Lake restoration is a part of geoengineering, which is a useful tool for landscape management. The phosphorus inactivation method is one of the most popular lake restoration methods. Using chemical compounds for P binding is leading to the creation of sediment “active layer”, which should show higher P adsorption abilities, compared to non-modified sediment. Howewer, it provides rather little information, how long the modified sediment remains active, and whether it is effective in continuous P binding. Lake meromixis is not commonly observed phenomenon, and sediment located in monimolimnion area is subjected long term anoxia. Therefore, observation of “active layer” in a meromictic lake can give very important data about durability of restoration effects. The object of our study was meromictic Starodworskie Lake (5.57 ha, max. depth 24.5 m), located in Olsztyn Lakeland, Poland. In the past the analyzed lake was subjected to various restoration methods, and phosphorus inactivation method by alum use (1994–1995) was the last used treatment type. The mixing regime of this lake had changed from bradimictic (before and during restoration time) into durable meromictic (post-restoration period). The research made two decades after implementing of P inactivation showed the presence of “active” sediment layer 10–15 cm below sediment surface. This sediment layer showed much higher content of P bound to aluminum, compared to surficial sediment layer. P binding molar ratio was assessed and amounted to 16.1 straightly after restoration and 6.1 after 21 years. This fraction amounts were higher that the values noted before restoration (ca. 358% higher than in 1994) and during restoration (ca. 86% higher than in 1995), which was probably the effect of continuous phosphorus adsorption by “active layer” in post-restoration period.

In deep lakes, many investigations highlighted the existence of exclusive groups of bacteria adapted to deep oxygenated and hypoxic and anoxic hypolimnia. Nevertheless, the extent of bacterial strain diversity has been much less scrutinized. This aspect is essential for an unbiased estimation of genetic variation, biodiversity, and population structure, which are essential for studying important research questions such as biogeographical patterns, temporal and spatial variability and the environmental factors affecting this variability. This study investigated the bacterioplankton community in the epilimnetic layers and in the oxygenated and hypoxic/anoxic hypolimnia of five large and deep lakes located at the southern border of the Alps using high throughput sequencing (HTS) analyses (16S rDNA) and identification of amplicon sequence variants (ASVs) resolving reads differing by as little as one nucleotide. The study sites, which included two oligomictic (Garda and Como) and three meromictic lakes (Iseo, Lugano, and Idro) with maximum depths spanning from 124 to 410 m, were chosen among large lakes to represent an oxic-hypoxic gradient. The analyses showed the existence of several unique ASVs in the three layers of the five lakes. In the case of cyanobacteria, this confirmed previous analyses made at the level of strains or based on oligotyping methods. As expected, the communities in the hypoxic/anoxic monimolimnia showed a strong differentiation from the oxygenated layer, with the exclusive presence in single lakes of several unique ASVs. In the meromictic lakes, results supported the hypothesis that the formation of isolated monimolimnia sustained the development of highly diversified bacterial communities through ecological selection, leading to the establishment of distinctive biodiversity zones. The genera identified in these layers are well-known to activate a wide range of redox reactions at low O2 conditions. As inferred from 16S rDNA data, the highly diversified and coupled processes sustained by the monimolimnetic microbiota are essential ecosystem services that enhance mineralization of organic matter and formation of reduced compounds, and also abatement of undesirable greenhouse gasses. [ABSTRACT FROM AUTHOR]

The microbial ecosystem of the meromictic Lake Cadagno (Ticino, Swiss Alps) has been studied intensively in order to understand structure and functioning of the anoxygenic phototrophic sulfur bacteria community living in the chemocline. It has been found that the purple sulfur bacterium " Thiodictyon syntrophicum " strain Cad16T, belonging to the Chromatiaceae, fixes around 26% of all bulk inorganic carbon in the chemocline, both during day and night. With this study, we elucidated for the first time the mode of carbon fixation of str. Cad16T under micro-oxic conditions with a combination of long-term monitoring of key physicochemical parameters with CTD, 14C-incorporation experiments and quantitative proteomics using in-situ dialysis bag incubations of str. Cad16T cultures. Regular vertical CTD profiling during the study period in summer 2017 revealed that the chemocline sank from 12 to 14 m which was accompanied by a bloom of cyanobacteria and the subsequent oxygenation of the deeper water column. Sampling was performed both day and night. CO2 assimilation rates were higher during the light period compared to those in the dark, both in the chemocline population and in the incubated cultures. The relative change in the proteome between day and night (663 quantified proteins) comprised only 1% of all proteins encoded in str. Cad16T. Oxidative respiration pathways were upregulated at light, whereas stress-related mechanisms prevailed during the night. These results indicate that low light availability and the co-occurring oxygenation of the chemocline induced mixotrophic growth in str. Cad16T. Our study thereby helps to further understand the consequences micro-oxic conditions for phototrophic sulfur oxidizing bacteria. The complete proteome data have been deposited to the ProteomeXchange database with identifier PXD010641. [ABSTRACT FROM AUTHOR]

In Quaternary paleosciences, the rationale behind analogical inference presupposes that former processes can be explained by causes operating now, although their intensity and rates can vary through time. In this paper we synthesised the results of different modern analogue studies performed in a varved lake. We discuss their potential value to obtain best results from high resolution past records. Different biogeochemical contemporary processes revealed seasonality and year-to-year variability, e.g., calcite precipitation, lake oxygenation, production and deposition of pollen and phytoplankton growth. Fingerprints of the first two of these processes were clearly evidenced in the varve-sublayers and allow understanding related to past events. Pollen studies suggested the possibility of identifying and characterizing seasonal layers even in the absence of varves. Marker pigments in the water column were tightly associated with phytoplankton groups living today; most of them were identified in the sediment record as well. We observed that 50% of these marker pigments were destroyed between deposition and permanent burying. In another study, seasonality in the production/distribution of branched glycerol dialkyl glycerol tetraethers (brGDGTs) and derived temperature estimates were investigated in catchment soils and particles settling in the lake. The signatures of brGDGTs in depositional environments mainly were representative of stable conditions of soils in the catchment that last over decades; no brGDGTs seemed to be produced within the lake. The main contribution of this review is to show the advantages and limitations of a multiproxy modern-analogue approach in Lake Montcortès as a case study and proposing new working hypotheses for future research.

This paper reports a study of oxygen and redox conditions, trophic status, and phytoplankton community in the meromictic Lake Idro (Italy) from 2010 to 2014. The sequence of causes and effects of meromixis are also evaluated by comparing recent research with studies conducted from the late 1960s to the mid-1990s. In the last half century, Lake Idro was steadily meromictic due to solutes which accumulated in its deep waters, along with both dissolved nutrients and chemically reduced substances produced by the anaerobic microbial metabolism. These substances were retained in bottom waters and made unavailable to upper layers until stratification broke. Mixing episodes occurred in 2005-2006 altering stratification, and oxygen and nutrient distribution within the lake. The potential full overturn effects were also evaluated as potential oxygen consumption due to the oxidation of reduced substances to forecast possible oxygen exhaustion and collapse of biological communities. Finally, meromixis is discussed as a potential threat for deep perialpine lakes using Lake Idro as a reference to comparatively evaluate the present status and possible future trends. [ABSTRACT FROM AUTHOR]

Extreme environmental conditions are key factors in the formation of the structure and diversity of microbial communities. In meromictic ecosystems, extreme conditions and a stable stratification of physical, chemical and biological parameters lead to diversity and heterogeneity of microenvironments. Lake Doroninskoe is located in an extreme geographical area and differs from other known meromictic reservoirs of the world by a low level of illumination in the chemocline and a rare type of alkaline water in sedimentary rocks formed by evaporative concentration. To understand the key factors that shape the composition and structure of the microbial community, the macro- and micro-variations in space and time are of great importance. We investigated the short-term dynamics of the structure and diversity of microbial communities of the meromictic soda lake, Lake Doroninskoe, at day and night using highthroughput sequencing and bioinformatics. Metagenomic analysis of 16S rRNA gene amplicons showed that the microbial communities had a high taxonomic diversity both at day and night. Sixteen bacterial and three archaeal phyla were identified. Proteobacteria were dominant and comprised 75% during the day, increasing to 90% at night. Metabolically stable denitrifying bacteria that were able to use a variety of alternative electron acceptors and electron donors were prevalent in Lake Doroninskoe. They belonged to the families Enterobacteriaceae (class Gammaproteobacteria) and Alcaligenaceae (class Betaproteobacteria). Statistically significant differences between day and night microbial communities were found. During the day, the microbial community was the most diverse. We discuss the peculiarities of the underexplored shortterm dynamics of the structure and diversity of the microbial communities of the meromictic soda Lake Doroninskoe, and propose topics for prospective studies. [ABSTRACT FROM AUTHOR]

The seasonal dynamics of the vertical structure of small saline Lake Uchum, located in the steppe arid zone of the south of Siberia (Krasnoyarsk krai), has been studied in detail for the first time. This lake is a meromictic water body. We have revealed a heterogeneous vertical distribution of plankton organisms and a dense population of purple sulfuric bacteria in the redox zone. The taxonomic composition and seasonal dynamics of phyto- and zooplankton are described. Presumably, the meromixis of Lake Uchum is due to the inflow of fresh water to the surface of the saline water body during the rise of its level in the early 20th century, similarly to lakes Shira and Shunet located nearby. The processes of salt displacement into the solution during the formation of ice, as well as the precipitation of salts in the winter, also contribute to the maintenance of permanent stratification. The information on the current state of the lake can be useful for reconstructing the climate by bottom sediments, as well as for creating a model of water quality and investigating the therapeutic properties of lake mud. [ABSTRACT FROM AUTHOR]

Recent expansion of anoxia has become a global issue and there is potential for worsening under global warming. At the same time, obtaining proper long-term instrumental oxygen records is difficult, thus reducing the possibility of recording long-term changes in oxygen shifts that can be related with climate or human influence. Varved lake sediments provide the better time frame to study this phenomenon at high resolution. We tracked the oxic/anoxic shifts of the varved Lake Montcortès since 1500 CE, and tried to recognise anthropogenic and climatic influences combining biological and geochemical proxies. Four main scenarios emerged: 1) years with abrupt sediment inputs (A); 2) years with outstanding mixing and oxygenation of the water column (B); 3) years with strong stratification, anoxia, intense sulfur bacterial activity and increased biomass production (C); 4) years with stratification and anoxia, but relatively less biomass production (D). In line with current limnologic trends, high supra-annual variability in the occurrence of oxygenation events was observed. Interestingly, at least 45.3% of the years were mixing years and, like the meromictic ones, were mostly clustered into groups of consecutive years, thus alternating years of monomixis with years of meromixis. Most years of D belong to the period 1500–1820 CE, when human activities were the most intense. Most years of A belonged to the climatic unstable period of 1850–1899 CE. Years of B were irregularly distributed but were best represented in the period 1820–1849 CE. Most years of C belonged to the 20th century. More than 90% of the years with climatic instrumental records belonged to B and C. Current climate warming seems to be taking control over the oxygenation capacity of the lake, especially since the second half of the 20th century. Our results support recent findings related to hypoxia spreading at the global scale. [ABSTRACT FROM AUTHOR]

We analysed the spatial and temporal variability of benthic nitrogen fluxes and denitrification rates in a sub-alpine meromictic lake (Lake Idro, Italy), and compared in-lake nitrogen retention and loss with the net anthropogenic nitrogen inputs to the watershed. We hypothesized a low nitrogen retention and denitrification capacity due to meromixis. This results from nitrate supply from the epilimnion slowing down during stratification and oxygen deficiency inhibiting nitrification and promoting ammonium recycling and its accumulation. We also hypothesized a steep vertical gradient of sedimentary denitrification capacity, decreasing with depth and oxygen deficiency. These are important and understudied issues in inland waters, as climate change and direct anthropic pressures may increase the extent of meromixis. Nearshore sediments had high denitrification rates (87 mg m day) and efficiency (~ 100%), while in the monimolimnion denitrification was negligible. The littoral zone, covering 10% of the lake surface, contributed ~50% of total denitrification, while the monimolimnion, which covered 70% of the sediment surface, contributed to < 13% of total denitrification. The persistent and expanding meromixis of Lake Idro is expected to further decrease its nitrogen removal capacity (31% of the incoming nitrogen load) compared to what has been measured in other temperate lakes. Values up to 60% are generally reported for other such lakes. Results of this study are relevant as the combination of anthropogenic pressures, climate change and meromixis may threaten the nitrogen processing capacity of lakes. [ABSTRACT FROM AUTHOR]

Assemblages of dinoflagellate cysts in lakebed sediments across the deep, meromictic basin of Sluice Pond (Lynn, Massachusetts) were compared with measurements of water quality and sediment geochemistry and with testate amoeba and tintinnid assemblages. Lakebed sediments below oxygenated waters (DO >2 mg/L) contain diverse testate amoebae, but sparsePeridinium willei-dominated dinoflagellate cyst assemblages. Sediments collected under hypoxic conditions (DO < 2 mg/L) showed higher preservation of organic carbon and nitrogen, and these deep basin sediments are characterised by diverse dinoflagellate cyst assemblages, but sieved microfossil assemblages are typically dominated by the planktonicCodonella crateraand the pseudo-planktonicCucurbitella tricuspis. Strong inverse relationships of cysts ofPeridinium willei(R2= 0.81) andDifflugia oblongatests (R2= 0.7) with water depth are attributed to variations in bottom water oxygen concentrations. Both ecological and taphonomic factors must be considered when reconstructing palaeoenvironments from microfossil assemblages. [ABSTRACT FROM AUTHOR]

Saline Lake Shira (Southern Siberia, Russia) was meromictic through the observation period 2002–2015. During the under-ice periods of 2015 and 2016, complete mixing of the water column was recorded for the first time, and hydrogen sulphide temporarily disappeared from the water column of the lake; i.e. in those years the lake turned to holomixis. In the summer of 2015, a sharp increase in chlorophyll a , organic carbon, zooplankton, and phytoflagellates was observed in the lake, which was probably due to the release of nutrients from the monimolimnion. Purple sulfur bacteria completely disappeared from the lake after the first mixing in 2015, and did not reappear despite the restoration of meromixis in 2017. Thus, it was demonstrated that purple sulfur bacteria are sensitive to the weakening of the stratification of Lake Shira. Based on the data of the seasonal monitoring of temperature and salinity profiles over the period 2002–2017, it was presumed that the main cause of deep mixing in 2015 was the weakening of the salinity gradient due to strong wind impact and early ice retreat in the spring of 2014. In addition, it was shown that in previous years a significant contribution to the maintenance of meromixis was made by an additional influx of fresh water, which caused a rise in the lake level in the period 2002–2007. Thus, we identified a relationship between the stratification regime of the lake and the change in its level, which provides valuable information both for the forecast of water quality and for reconstruction of the Holocene climate humidity in this region of Southern Siberia from the sediment cores of Lake Shira. [ABSTRACT FROM AUTHOR]

Reliable gas measurements from supersaturated deep waters still remain a challenge. However, good information is mandatory to investigate the limnic carbon cycle, assess the endangerment through limnic eruptions and evaluate a potential source of exploitable energy. We addressed these three points in a heavily polluted mine pit lake in Germany. We quantified the ebullition of methane from deep waters and the sediment below. Exposed to continuous percolation of gas bubbles, the deep (monimolimnetic) water had accumulated high concentrations of gas: directly measured gas pressures indicated the proximity to spontaneous ebullition. Consequently, the possibility of a limnic eruption was assessed by initiating a self-sustained flow through a vertical pipe. Despite the high gas pressures, the flow was slow and the endangerment was considered low. A sampling strategy with bags was developed to achieve a reliable measurement of gas content and gas composition in the monimolimnion. As a result, directly measured gas pressures could be confirmed and were nearly exclusively attributed to methane and nitrogen. Contrary to lakes that had shown limnic eruptions, carbon dioxide played a much subordinate role, and hence the driving force for a violent outburst of gases was missing. Nevertheless the amount of dissolved methane was remarkably high. This investigation closes with some estimates of the commercial value of the deposit and limiting conditions for a possible exploitation. [ABSTRACT FROM AUTHOR]

We used monitoring data from Lake Lugano (Switzerland and Italy) to assess key ecosystem responses to three decades of nutrient management (1983–2014). We investigated whether reductions in external phosphorus loadings (L ext ) caused declines in lake phosphorus concentrations (P) and phytoplankton biomass (Chl a ), as assumed by the predictive models that underpinned the management plan. Additionally, we examined the hypothesis that deep lakes respond quickly to L ext reductions. During the study period, nutrient management reduced L ext by approximately a half. However, the effects of such reduction on P and Chl a were complex. Far from the scenarios predicted by classic nutrient-management approaches, the responses of P and Chl a did not only reflect changes in L ext , but also variation in internal P loadings (L int ) and food-web structure. In turn, L int varied depending on basin morphometry and climatic effects, whereas food-web structure varied due to apparently stochastic events of colonization and near-extinction of key species. Our results highlight the complexity of the trajectory of deep-lake ecosystems undergoing nutrient management. From an applied standpoint, they also suggest that [ i ] the recovery of warm monomictic lakes may be slower than expected due to the development of L int , and that [ ii ] classic P and Chl a models based on L ext may be useful in nutrient management programs only if their predictions are used as starting points within adaptive frameworks. [ABSTRACT FROM AUTHOR]

Lakes represent a considerable natural source of methane to the atmosphere compared to their small global surface area. Methanotrophs in sediments and in the water column largely control methane fluxes from these systems, yet the diversity, electron accepting capacity and nutrient requirements of these microorganisms have only been partially identified. Here we investigated the role of electron acceptors alternative to oxygen and sulfate in microbial methane oxidation at the oxycline and in anoxic waters of the ferruginous meromictic Lake La Cruz, Spain. Active methane turnover in a zone extending well below the oxycline was evidenced by stable carbon isotope-based rate measurements. We observed a strong methane oxidation potential throughout the anoxic water column, which did not vary substantially from that at the oxic/anoxic interface. Both in the redox-transition and anoxic zones, only aerobic methane-oxidizing bacteria were detected by fluorescence in situ hybridization and sequencing techniques, suggesting a close coupling of cryptic photosynthetic oxygen production and aerobic methane turnover. Additions of nitrate, nitrite and to a lesser degree iron and manganese oxides also stimulated bacterial methane consumption. We could not confirm a direct link between the reduction of these compounds and methane oxidation and we cannot exclude the contribution of unknown anaerobic methanotrophs. Nevertheless, our findings from Lake La Cruz support recent laboratory evidence that aerobic methanotrophs may be able to utilize alternative terminal electron acceptors under oxygen limitation.

AIM: chemical stratification and the patterns of light limitation and nutrients of two natural lakes, one shallow and the other one deep, were comparatively evaluated, both lakes located in the southeast Brazil. METHODS: pH, electrical conductivity, dissolved oxygen, total dissolved solids and nutrients were monthly collected during 5 consecutive years at the vertical profile of the two lakes. RESULTS: multivariate analysis indicated that the long thermal stratification period favored the occurrence of chemical stratification in the two lakes. However, in the deeper lake the stratified thermal profile with high hypolimnetic nutrient concentration, electrical conductivity, total dissolved solids and redox potential indicated that the mixing was not complete even during the annual circulation period, suggesting a slight meromixis and a high chemical stability at the hypolimnion. In the shallower lake, high light attenuation and high availability of nitrogen forms (mainly N-NH4) and phosphorus was observed along the water column, even during stratification. In the deeper lake, N and P co-limitation and low light attenuation coefficients were detected. CONCLUSION: thermal and chemical stratification patterns indicated that the Carioca lake is a shallow, turbid, nutrient rich, whereas the Dom Helvecio lake is a deep, clear, oligotrophic system with a tendency towards meromixis and the isolation of solutes in the hypolimnion. Consequently, meromixis was compared to a "hypolimnetic memory", which was defined, in the case of the deeper lake, as the maintenance of the chemical stratification along the years, during the lake thermal circulation period.OBJETIVO: a estratificação química e os padrões de limitação por luz e nutrientes foram avaliados comparativamente em dois lagos naturais, sendo um raso e o outro profundo no sudeste do Brasil. MÉTODOS: as amostragens de pH, condutividade elétrica, oxigênio dissolvido, sólidos totais dissolvidos e nutrientes foram realizadas mensalmente, durante cinco anos consecutivos no perfil vertical dos dois lagos. RESULTADOS: as análises multivariadas indicaram que o longo período de estratificação térmica favoreceu a ocorrência de estratificação química nos dois lagos. No entanto, no lago mais profundo, o perfil térmico estratificado, com elevadas concentrações hipolimnéticas de nutrientes, condutividade, sólidos totais dissolvidos e potencial redox indicaram que a mistura não foi completa mesmo durante o período de circulação anual, sugerindo uma leve meromixia e alta estabilidade química no hipolímnio. No lago mais raso, elevada atenuação de luz e alta disponibilidade das formas nitrogenadas (principalmente N-NH4) e fosfatadas foram observadas em toda a coluna d'água, mesmo durante a estratificação. No lago mais profundo, foram observadas co-limitação por N e P e baixas coeficientes de atenuação da luz. CONCLUSÃO: os padrões de estratificação térmica e química indicaram que a lagoa Carioca é um lago raso, de rápida circulação com águas túrbidas, rico em nutrientes, enquanto o lago Dom Helvécio é um lago profundo, de águas claras, oligotrófico, com tendência à meromixia e ao isolamento de solutos no hipolímnio. Nesse contexto, a meromixia foi comparada a uma "memória hipolimnética" que foi definida, no caso do lago mais profundo, como a persistência da estratificação química ao longo dos anos durante o período de sua circulação térmica.

Chemical characteristics of permanent stratification in cave pools (meromixis) may provide insight into the geochemical origin and evolution of cave pool waters. The objective of this study was to test the hypothesis that some pools in Lechuguilla Cave may be subject to ectogenic meromixis, where permanent chemical stratification is induced by input of relatively saline or fresh water from an external source. However, because organic C concentrations in Lechuguilla waters are low (typically < 1 mg L-1), biogenic meromixis resulting in O2(g)-depleted subsurface waters is not expected. Four pools at various depths below ground surface (0 m) were studied: (1) Lake Chandalar (-221 m), (2) Lake of the Blue Giants (LOBG) (- 277 m), (3) Lake Margaret (- 319 m), and (4) Lake of the White Roses (LOWR) (- 439 m). Water column profiles of temperature, pH, dissolved O2(g), and electrical conductivity (EC) were collected down to a maximum depth of 13.1 m using a multi-parameter sonde. Opposite pH gradients were observed at Lake Chandalar and LOBG, where pH at the surface (0.3 m) varied by ±0.20 units compared to the subsurface (> 0.9 m), and are probably the result of localized and transient atmospheric CO2(g) concentrations. At LOBG, an EC increase of 93 µS cm-1 at the 0.9-m depth suggests meromictic conditions which are ectogenic, possibly due to surface inflow of fresh water as drips or seepage into a pre-existing layer of higher salinity.

Lakes represent a considerable natural source of methane to the atmosphere compared to their small global surface area. Methanotrophs in sediments and in the water column largely control methane fluxes from these systems, yet the diversity, electron accepting capacity, and nutrient requirements of these microorganisms have only been partially identified. Here, we investigated the role of electron acceptors alternative to oxygen and sulfate in microbial methane oxidation at the oxycline and in anoxic waters of the ferruginous meromictic Lake La Cruz, Spain. Active methane turnover in a zone extending well below the oxycline was evidenced by stable carbon isotope-based rate measurements. We observed a strong methane oxidation potential throughout the anoxic water column, which did not vary substantially from that at the oxic/anoxic interface. Both in the redox-transition and anoxic zones, only aerobic methane-oxidizing bacteria (MOB) were detected by fluorescence in situ hybridization and sequencing techniques, suggesting a close coupling of cryptic photosynthetic oxygen production and aerobic methane turnover. Additions of nitrate, nitrite and to a lesser degree iron and manganese oxides also stimulated bacterial methane consumption. We could not confirm a direct link between the reduction of these compounds and methane oxidation and we cannot exclude the contribution of unknown anaerobic methanotrophs. Nevertheless, our findings from Lake La Cruz support recent laboratory evidence that aerobic methanotrophs may be able to utilize alternative terminal electron acceptors under oxygen limitation. [ABSTRACT FROM AUTHOR]

Meromictic soda and saline lakes are unique ecosystems characterized by the stability of physical, chemical and biological parameters, and they are distributed all over the world. Lakes located in regions with average annual negative air temperature are of particular interest because of the presence of two periods with intensive and dynamic processes: the so-called biological summer and the long ice season with the biological spring. Soda Lake Doroninskoe is located in Eastern Transbaikalia (51°14′N, 112°14′E) in the permafrost zone in an extreme continental climate, and is covered by ice for seven months per year. The structure and diversity of the microbial communities throughout the water column of the lake was studied by 16S rRNA gene amplicon metasequencing. Different species with specific functions were found to dominate at different depths. Metabolically flexible bacteria with a capacity to switch between anoxygenic photosynthesis and aerobic chemotrophic metabolism dominate in soda Lake Doroninskoe. [ABSTRACT FROM AUTHOR]

There are numerous anthropogenic water reservoirs in the vicinity of the Žulovská pahorkatina (northern Czech Republic). The vast majority of them have developed as a result of the flooding of the abandoned quarries of crystalline rocks (granites or granodiorites). The surface area of these water bodies is small and is <6000 m. These are, however, very deep reservoirs of up to 25 m. Permanent water stratification has been recorded in some of them; these are meromictic reservoirs. The development of the phenomenon of meromixis in the reservoirs that were studied was conditioned by the supply of organic matter from the catchment. The biochemical process of organic matter decomposition led to the release of ions, mainly NH. The accumulation of dissolved substances in the benthic zone initiated the meromixis phenomenon. Therefore, the water bodies that were tested can be classified as reservoirs of the biogenic type of meromixis. This article presents the stages of the evolution of a holomictic reservoir into a meromictic one. Three water bodies in which meromixis was recorded were selected for the study. The control object was an abandoned quarry reservoir with no meromixis. [ABSTRACT FROM AUTHOR]

The data on changes in the vertical distribution of free-living ciliates from a freshwater meromictic pond (city of Samara) within a year are presented. The specific character of the waterbody is that the taxonomic diversity of ciliates of pelagic plankton is mainly formed not by usual euplanktonic species, but by ciliates of highly specialized ecological groups such as mixotrophs, 'benthic migrants,' and sapropelic species. The ciliate fauna is similar to the fauna of thermally stratified lakes with anaerobic hypolimnion. The maximal abundance and biomass of ciliates during the year and their high absolute values are determined by the existence of stable gradients of abiotic factors and, in the first turn, concentrations of oxygen, hydrogen sulfide, and oxidation-reduction potential. [ABSTRACT FROM AUTHOR]

Saline runoff from application of road salt deicers has the potential to create persistent density gradients in urban freshwater lakes, resulting in significant changes to physical mixing and biogeochemical cycling. In this study, the seasonal influence of road salt influx on the geochemical cycling of a small, urban kettle lake is examined. Water column samples were collected approximately twice per month for fifteen months and analyzed for temperature, pH, conductivity, major ions, nutrients and redox-sensitive solutes. The lake water column was redox-stratified with high concentrations of dissolved Mn(II), Fe(II), ammonium, phosphate and, during some periods, sulfide and methane in the hypolimnion. Bottom water anoxia was persistent throughout the entire sampling period. Concentrations of sodium and chloride, which correlated strongly with conductivity, were over a hundred times greater compared with levels in nearby rural lakes. Conductivity, chloride and calculated density profiles indicated the presence of a pycnocline at ~ 9 m depth that persisted throughout the sampling period. The chemical data demonstrate that road salt input has caused the lake to become meromictic, with a complete lack of fall and spring turnover. [ABSTRACT FROM AUTHOR]