Search

Your search keyword '"Meresta, Anna"' showing total 8 results
Start Over Author "Meresta, Anna"
8 results on '"Meresta, Anna"'

1. Detection of antibiotic resistance genes in wastewater treatment plant – molecular and classical approach

Author

Ziembińska-Buczyńska Aleksandra, Felis Ewa, Folkert Justyna, Meresta Anna, Stawicka Dominika, Gnida Anna, and Surmacz-Górska Joanna

Subjects
DGGE, PCR, sulfamethoxazole, trimethoprim and erythromycin resistance, WWTP, Environmental protection, TD169-171.8
Abstract

Antibiotics are a group of substances potentially harmful to the environment. They can play a role in bacterial resistance transfer among pathogenic and non-pathogenic bacteria. In this experiment three representatives of medically important chemotherapeutics, confirmed to be present in high concentrations in wastewater treatment plants with HPLC analysis were used: erythromycin, sulfamethoxazole and trimethoprim. Erythromycin concentration in activated sludge was not higher than 20 ng L−1. N-acetylo-sulfamethoxazole concentration was 3349 ± 719 in winter and 2933 ± 429 ng L−1 in summer. Trimethoprim was present in wastewater at concentrations 400 ± 22 and 364 ± 60 ng L−1, respectively in winter and summer. Due to a wide variety of PCR-detectable resistance mechanisms towards these substances, the most common found in literature was chosen. For erythromycin: erm and mef genes, for sulfamethoxazole: sul1, sul2, sul3 genes, in the case of trimethoprim resistance dhfrA1 and dhfr14 were used in this study. The presence of resistance genes were analyzed in pure strains isolated from activated sludge and in the activated sludge sample itself. The research revealed that the value of minimal inhibitory concentration (MIC) did not correspond with the expected presence of more than one resistance mechanisms. Most of the isolates possessed only one of the genes responsible for a particular chemotherapeutic resistance. It was confirmed that it is possible to monitor the presence of resistance genes directly in activated sludge using PCR. Due to the limited isolates number used in the experiment these results should be regarded as preliminary.

Published
2015
Full Text
View/download PDF

2. Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

Author

Meresta, Anna, Folkert, Justyna, Gaber, Timo, Miksch, Korneliusz, Buttgereit, Frank, Detert, Jacqueline, Pischon, Nicole, and Gurzawska, Katarzyna

Subjects
Inflammation, nanocoatings, Osteoblasts, Real-Time Polymerase Chain Reaction, Mice, Inbred C57BL, Coated Materials, Biocompatible, Gene Expression Regulation, International Journal of Nanomedicine, Animals, Nanoparticles, Pectins, Porphyromonas gingivalis, Cell Shape, Cells, Cultured, Original Research, Rhamnogalacturonan-I, Cell Proliferation, Solanum tuberosum
Abstract

Anna Meresta,1 Justyna Folkert,1 Timo Gaber,2 Korneliusz Miksch,1 Frank Buttgereit,2 Jacqueline Detert,2 Nicole Pischon,3,* Katarzyna Gurzawska3,4,* 1Environmental Biotechnology Department, Faculty of Power and Environmental, Silesian University of Technology, Gliwice, Poland; 2Department of Rheumatology and Clinical Immunology, 3Department of Periodontology, Charité University Medicine, Berlin, Germany; 4Oral Surgery Department, The School of Dentistry, University of Birmingham, Birmingham, UK *These authors contributed equally tothis work Background: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.Methods: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.Results: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa.Conclusion: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders. Keywords: nanocoatings, Rhamnogalacturonan-I, Porphyromonas gingivalis, osteoblasts, inflammation

Published
2017

3. Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

Author

Meresta,Anna, Folkert,Justyna, Gaber,Timo, Miksch,Korneliusz, Buttgereit,Frank, Detert,Jacqueline, Pischon,Nicole, Gurzawska,Katarzyna, Meresta,Anna, Folkert,Justyna, Gaber,Timo, Miksch,Korneliusz, Buttgereit,Frank, Detert,Jacqueline, Pischon,Nicole, and Gurzawska,Katarzyna

Abstract

Anna Meresta,1 Justyna Folkert,1 Timo Gaber,2 Korneliusz Miksch,1 Frank Buttgereit,2 Jacqueline Detert,2 Nicole Pischon,3,* Katarzyna Gurzawska3,4,* 1Environmental Biotechnology Department, Faculty of Power and Environmental, Silesian University of Technology, Gliwice, Poland; 2Department of Rheumatology and Clinical Immunology, 3Department of Periodontology, Charité University Medicine, Berlin, Germany; 4Oral Surgery Department, The School of Dentistry, University of Birmingham, Birmingham, UK *These authors contributed equally to this work Background: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.Methods: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.Results: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregula

Published
2017

4. Nanocoating with plant-derived pectins activates osteoblast response in vitro

Author

Folkert,Justyna, Meresta,Anna, Gaber,Timo, Miksch,Korneliusz, Buttgereit,Frank, Detert,Jacqueline, Pischon,Nicole, Gurzawska,Katarzyna, Folkert,Justyna, Meresta,Anna, Gaber,Timo, Miksch,Korneliusz, Buttgereit,Frank, Detert,Jacqueline, Pischon,Nicole, and Gurzawska,Katarzyna

Abstract

J Folkert,1 A Meresta,1 T Gaber,2 K Miksch,1 F Buttgereit,2 J Detert,2 N Pischon,3,* K Gurzawska3,4,* 1Environmental Biotechnology Department, Faculty of Power and Environmental, Silesian University of Technology, Gliwice, Poland; 2Department of Rheumatology and Clinical Immunology, 3Department of Periodontology, Charité-Universitätsmedizin, Berlin, Germany; 4Department of Oral Surgery, The School of Dentistry, University of Birmingham, Birmingham, UK *These authors contributed equally to this work Abstract: A new strategy to improve osseointegration of implants is to stimulate adhesion of bone cells, bone matrix formation, and mineralization at the implant surface by modifying surface coating on the nanoscale level. Plant-derived pectins have been proposed as potential candidates for surface nanocoating of orthopedic and dental titanium implants due to 1) their osteogenic stimulation of osteoblasts to mineralize and 2) their ability to control pectin structural changes. The aim of this study was to evaluate in vitro the impact of the nanoscale plant-derived pectin Rhamnogalacturonan-I (RG-I) from potato on the osteogenic response of murine osteoblasts. RG-I from potato pulps was isolated, structurally modified, or left unmodified. Tissue culture plates were either coated with modified RG-I or unmodified RG-I or – as a control – left uncoated. The effect of nanocoating on mice osteoblast-like cells MC3T3-E1 and primary murine osteoblast with regard to proliferation, osteogenic response in terms of mineralization, and gene expression of Runt-related transcription factor 2 (Runx2), alkaline phosphate (Alpl), osteocalcin (Bglap), α-1 type I collagen (Col1a1), and receptor activator of NF-κB ligand (Rankl) were analyzed after 3, 7, 14, and 21 days, respectively. Nanocoating with pectin RG-Is increased proliferation and mineralization of MC3T3-E1 and primary osteoblast as compared to osteoblast

Published
2016

7. INFLUENCE OF THE DURATION OF FEEDING PHASE ON THE GENOTYPIC STRUCTURE OF BACTERIAL COMMUNITIES IN TWO SEQUENCING BATCH REACTORS TREATING REJECT WATER BY PARTIAL NITRITATION AND ANAMMOX.

Author

ZIEMBIŃSKA-BUCZYŃSKA, ALEKSANDRA, CEMA, GRZEGORZ, MERESTA, ANNA, and PŁONKA, LESŁAW

Subjects
BACTERIAL communities, BATCH reactors, SEQUENCING batch reactor process, GEL electrophoresis, GENOTYPES
Abstract

Microbial community and physiochemical processes were monitored by means of denaturing gradient gel electrophoresis (PCR-DGGE) in two sequencing batch reactors (SBRs) running for partial nitritationanammox and differing in the feeding phase durations (6 h 40 min for SBR1 and 40 min for SBR2). Both SBRs were treated with reject water with a high ammonia concentration (>600 mg NH4+/dm³) for over 370 days. The aim of the experiment was to present the influence of this parameter on total bacterial and anammox bacterial community structure. Molecular analysis revealed that a drastic decrease in influent ammonia concentration to the studied communities caused a change of genotypic structure in their composition. The difference in the reactors working scheme can be the reason for divergence in the community structure though having no drastic influence on its performance and biodiversity level. Feeding time has stronger influence on the genotypic composition of the total bacterial community than on anammox biocenosis. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

8. Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

Author

Meresta, Anna, Folkert, Justyna, Gaber, Timo, Miksch, Korneliusz, Buttgereit, Frank, Detert, Jacqueline, Pischon, Nicole, and Gurzawska, Katarzyna

Subjects
nanocoatings, inflammation, osteoblasts, 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit, Porphyromonas gingivalis, 3. Good health, Rhamnogalacturonan-I
Abstract

Background: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection. Methods: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined. Results: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa. Conclusion: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders.

Catalog

Books, media, physical & digital resources
See catalog results