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516 results on '"Merayo-Lloves, Jesús"'

5. Cell Surface Glycosaminoglycans as Receptors for Adhesion of Candida spp. to Corneal Cells

Author

Ordiales Helena, Alcalde Ignacio, Vázquez Fernando, Merayo-Lloves Jesús, Quirós Luis M., and Cueto Carla Martín

Subjects
fungal keratitis, cornea, glycosaminoglycan, heparan sulfate, chondroitin sulfate, Genetics, QH426-470, Microbiology, QR1-502
Abstract

The most common causal agents of fungal keratitis are yeasts of the Candida genus. Adhesion constitutes the first stage of pathogenesis. Previous studies have shown that glycosaminoglycans from the corneal cell surface play an essential role in bacterial keratitis, although little is known about their role in fungal infections. The objective of this work is to analyze the role that glycosaminoglycans (GAGs) play in the adhesion of fungi of the Candida genus to corneal epithelial cells. The participation of GAGs in the adhesion of fungi was studied through the specific inhibition of the synthesis of these molecules by enzymatic digestion using specific lyases and the silencing of various genes involved in heparan sulfate sulfation. The results seem to indicate that glycosaminoglycans act to some extent as receptors for this fungus, although there are differences between fungal species. Treatment with inhibitors partially reduced the adherence of fungal species. Digestion of cell surface heparan sulfate further reduced the adherence of Candida albicans and Candida glabrata compared to chondroitin sulfate, indicating that the binding is preferentially mediated by heparan sulfate. Degradation of both heparan sulfate and chondroitin sulfate produced similar effects on the adherence of Candida parapsilosis. However, adhesion of C. albicans hyphae is not dependent on GAGs, suggesting the expression of other adhesins and the recognition of other receptors present in corneal cells. Our results open the door to new strategies for stopping the adhesion of pathogenic fungi, and their subsequent invasion of the cornea; thus, reducing the probability of the keratitis development.

Published
2022
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27. The Binding of Pseudomonas aeruginosa to Cystic Fibrosis Bronchial Epithelial Model Cells Alters the Composition of the Exosomes They Produce Compared to Healthy Control Cells.

Author

Lozano-Iturbe, Víctor, Blanco-Agudín, Noelia, Vázquez-Espinosa, Emma, Fernández-Vega, Iván, Merayo-Lloves, Jesús, Vazquez, Fernando, Girón, Rosa M., and Quirós, Luis M.

Subjects
CYSTIC fibrosis, QUORUM sensing, PSEUDOMONAS aeruginosa, CELL communication, EXOSOMES, EPITHELIAL cells, EXTRACELLULAR matrix proteins
Abstract

Cystic fibrosis (CF) is a genetic disease that causes dehydration of the surface of the airways, increasing lung infections, most frequently caused by Pseudomonas aeruginosa. Exosomes are nanovesicles released by cells that play an essential role in intercellular communication, although their role during bacterial infections is not well understood. In this article, we analyze the alterations in exosomes produced by healthy bronchial epithelial and cystic fibrosis cell lines caused by the interaction with P. aeruginosa. The proteomic study detected alterations in 30% of the species analyzed. In healthy cells, they mainly involve proteins related to the extracellular matrix, cytoskeleton, and various catabolic enzymes. In CF, proteins related to the cytoskeleton and matrix, in addition to the proteasome. These differences could be related to the inflammatory response. A study of miRNAs detected alterations in 18% of the species analyzed. The prediction of their potential biological targets identified 7149 genes, regulated by up to 7 different miRNAs. The identification of their functions showed that they preferentially affected molecules involved in binding and catalytic activities, although with differences between cell types. In conclusion, this study shows differences in exosomes between CF and healthy cells that could be involved in the response to infection. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Altered Clock Gene Expression in Female APP/PS1 Mice and Aquaporin-Dependent Amyloid Accumulation in the Retina.

Author

Carrero, Laura, Antequera, Desireé, Alcalde, Ignacio, Megias, Diego, Ordoñez-Gutierrez, Lara, Gutierrez, Cristina, Merayo-Lloves, Jesús, Wandosell, Francisco, Municio, Cristina, and Carro, Eva

Subjects
MOLECULAR clock, CLOCK genes, SUPRACHIASMATIC nucleus, GENE expression, AMYLOID, RETINA
Abstract

Alzheimer's disease (AD), the most prevalent form of dementia, is a neurodegenerative disorder characterized by different pathological symptomatology, including disrupted circadian rhythm. The regulation of circadian rhythm depends on the light information that is projected from the retina to the suprachiasmatic nucleus in the hypothalamus. Studies of AD patients and AD transgenic mice have revealed AD retinal pathology, including amyloid-β (Aβ) accumulation that can directly interfere with the regulation of the circadian cycle. Although the cause of AD pathology is poorly understood, one of the main risk factors for AD is female gender. Here, we found that female APP/PS1 mice at 6- and 12-months old display severe circadian rhythm disturbances and retinal pathological hallmarks, including Aβ deposits in retinal layers. Since brain Aβ transport is facilitated by aquaporin (AQP)4, the expression of AQPs were also explored in APP/PS1 retina to investigate a potential correlation between retinal Aβ deposits and AQPs expression. Important reductions in AQP1, AQP4, and AQP5 were detected in the retinal tissue of these transgenic mice, mainly at 6-months of age. Taken together, our findings suggest that abnormal transport of Aβ, mediated by impaired AQPs expression, contributes to the retinal degeneration in the early stages of AD. [ABSTRACT FROM AUTHOR]

Published
2023
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30. PINK1 Immunoexpression Predicts Survival in Patients Undergoing Hepatic Resection for Colorectal Liver Metastases

Author

Celis-Pinto, Juan Carlos, primary, Fernández-Velasco, Adela Alonso, additional, Corte-Torres, María Daniela, additional, Santos-Juanes, Jorge, additional, Blanco-Agudín, Noelia, additional, Piña Batista, Kelvin Manuel, additional, Merayo-Lloves, Jesús, additional, Quirós, Luis M., additional, and Fernández-Vega, Iván, additional

Published
2023
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40. A Simple, Safe, and Effective Method for Preparing Autologous Bio-Based Fibrin Glue for Ophthalmic Use

Author

Fernández-Vega-Cueto, Luis, primary, Persinal-Medina, Mairobi, additional, Vázquez, Natalia, additional, Chacón, Manuel, additional, Alfonso-Bartolozzi, Belén, additional, Alonso-Alonso, Sergio, additional, Sánchez, Teresa, additional, Berisa-Prado, Silvia, additional, Martínez-López, Laura María, additional, Merayo-Lloves, Jesús, additional, and Meana, Álvaro, additional

Published
2022
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47. Small fiber neuropathy in the cornea of Covid-19 patients associated with the generation of ocular surface disease

Author

Gallar, Juana [0000-0002-3559-3649], Fernández-Vega Cueto, Luis [0000-0001-9197-5847], Alfonso, José F. [0000-0002-0373-8209], Quirós, Luis M. [0000-0003-3505-3762], Barros, Alberto, Queiruga-Piñeiro, Juan, Lozano-Sanroma, Javier, Alcalde, Javier, Gallar, Juana, Fernández-Vega Cueto, Luis, Alfonso, José F., Quirós, Luis M., Merayo-Lloves, Jesús, Gallar, Juana [0000-0002-3559-3649], Fernández-Vega Cueto, Luis [0000-0001-9197-5847], Alfonso, José F. [0000-0002-0373-8209], Quirós, Luis M. [0000-0003-3505-3762], Barros, Alberto, Queiruga-Piñeiro, Juan, Lozano-Sanroma, Javier, Alcalde, Javier, Gallar, Juana, Fernández-Vega Cueto, Luis, Alfonso, José F., Quirós, Luis M., and Merayo-Lloves, Jesús

Abstract

[Purpose]: To describe the association between Sars-CoV-2 infection and small fiber neuropathy in the cornea identified by in vivo corneal confocal microscopy., [Methods]: Twenty-three patients who had overcome COVID-19 were recruited to this observational retrospective study. Forty-six uninfected volunteers were also recruited and studied as a control group. All subjects were examined under in vivo confocal microscopy to obtain images of corneal subbasal nerve fibers in order to study the presence of neuroma-like structures, axonal beadings and dendritic cells. The Ocular Surface Disease Index (OSDI) questionnaire and Schirmer tear test were used as indicators of Dry Eye Disease (DED) and ocular surface pathology., [Results]: Twenty-one patients (91.31%) presented alterations of the corneal subbasal plexus and corneal tissue consistent with small fiber neuropathy. Images from healthy subjects did not indicate significant nerve fiber or corneal tissue damage. Eight patients reported increased sensations of ocular dryness after COVID-19 infection and had positive DED indicators. Beaded axons were found in 82.60% of cases, mainly in patients reporting ocular irritation symptoms. Neuroma-like images were found in 65.22% patients, more frequently in those with OSDI scores >13. Dendritic cells were found in 69.56% of patients and were more frequent in younger asymptomatic patients. The presence of morphological alterations in patients up to 10 months after recovering from Sars-CoV-2 infection points to the chronic nature of the neuropathy., [Conclusions]: Sars-CoV-2 infection may be inducing small fiber neuropathy in the ocular surface, sharing symptomatology and morphological landmarks with DED and diabetic neuropathy.

Published
2022

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