11 results on '"Mengya Si"'
Search Results
2. Biomarkers Reflecting The Destruction Of The Blood-Brain Barrier Are Valuable In Predicting The Risk Of Lymphomas With Central Nervous System Involvement
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Ping He, Xiao-yang Jiao, Qiaoxin Zhang, Li Li, Mengya Si, Zhiqiang Fan, and Wenjun Yu
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0301 basic medicine ,tight junction protein ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Central nervous system ,lymphoma ,Blood–brain barrier ,OncoTargets and Therapy ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Cerebrospinal fluid ,medicine ,Pharmacology (medical) ,Original Research ,Tight junction ,business.industry ,medicine.disease ,cytokines ,Lymphoma ,030104 developmental biology ,medicine.anatomical_structure ,Cytokine ,Oncology ,CNS metastasis ,030220 oncology & carcinogenesis ,Csf biomarkers ,business - Abstract
Wenjun Yu,1,* Mengya Si,2,* Li Li,3,* Ping He,4 Zhiqiang Fan,4 Qiaoxin Zhang,2 Xiaoyang Jiao4 1Department of Hematology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, People’s Republic of China; 2Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, People’s Republic of China; 3Obstetrics Department, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, People’s Republic of China; 4Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaoyang JiaoDepartment of Cell Biology and Genetics, Shantou University Medical College, 22 Xinling Road, Guangdong 515041, People’s Republic of ChinaEmail xyjiao@stu.edu.cnObjective: We aimed to identify the biomarkers in cerebrospinal fluid (CSF) that facilitate the diagnosis of lymphomas with central nervous system (CNS) involvement.Methods: Four cases of non-Hodgkin’s lymphoma (NHL) patients with/without CNS involvement were enrolled respectively, and non-CNS tumor patients (n=3) were selected to be the controls. Lab biomarkers, cytokines, and tight junction proteins (TJs) in CSF and serum were measured.Results: When comparing the CNS to non-CNS group, cytokine including MMP-9 (15.24 vs 0.36 ng/mL), CCL-2 (1922.04 vs 490.68 pg/mL), and sVCAM-1 (61.36 vs 9.00 pg/mL), TJs including OCLN (6.68 vs 2.59 pg/mL), and ZO-1 (710.04 vs 182.98 pg/mL) in CSF were significantly higher in lymphomas patients with CNS involvement than those without CNS involvement. However, serum biomarkers were not significantly elevated. Contrary to the major findings, all conventional biomarkers and MRI results showed no significant change.Conclusion: CSF biomarkers affecting BBB disruption are valuable in mirroring the risk of lymphoma CNS metastasis. Further study with a larger sample size is needed to verify these biomarkers in predicting lymphoma CNS involvement.Keywords: lymphoma, CNS metastasis, cytokines, tight junction protein
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- 2019
3. The Correlation of Serums CCL11, CCL17, CCL26, and CCL27 and Disease Severity in Patients with Urticaria
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Jinbo Zou, Tao Lu, Xiaoyang Jiao, Mengya Si, Kang Zeng, Shuping Zhang, and Ping He
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Adult ,Chemokine CCL11 ,Male ,0301 basic medicine ,Chemokine ,Article Subject ,Urticaria ,Clinical Biochemistry ,Pathogenesis ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,immune system diseases ,parasitic diseases ,Genetics ,Humans ,Medicine ,CCL17 ,Young adult ,skin and connective tissue diseases ,Molecular Biology ,CCL11 ,lcsh:R5-920 ,biology ,Chemokine CCL26 ,business.industry ,Chemokine CCL27 ,Biochemistry (medical) ,General Medicine ,respiratory system ,Middle Aged ,030104 developmental biology ,030228 respiratory system ,Chemokines, CC ,Cohort ,Immunology ,biology.protein ,Female ,CCL27 ,Chemokine CCL17 ,CCL26 ,lcsh:Medicine (General) ,business ,Biomarkers ,Research Article - Abstract
Background.Chemokines may be involved in the pathogenesis of urticaria, but their correlation with disease severity as well as eruption type is unclear.Objectives.The aim of this study was to explore the expression of chemokines in patients with urticaria. The association between disease severity and levels of chemokines was analysed.Materials and Methods.Serums CCL11, CCL17, CCL26, and CCL27, D-dimer, C-reactive protein, and total IgE were measured in 51 patients with urticaria and in 25 healthy control subjects.Results.Serums CCL11, CCL17, CCL26, and CCL27 were significantly higher in patients with urticaria than in the healthy controls (P<0.05). Serum CCL27 strongly correlated with urticarial disease severity. Serums CCL17, CCL26, and CCL27 significantly correlated with D-dimer, while innercorrelations were noted among the chemokines.Conclusion.Our findings reveal that chemokines participate in the pathogenesis of urticaria. Further study in larger cohort is needed to testify whether they could be the biomarkers for predicting the severity of urticaria.
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- 2016
4. The role of cytokines and chemokines in the microenvironment of the blood–brain barrier in leukemia central nervous system metastasis
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Xiaoyang Jiao, Huanzhu Chen, Ping He, Yazhen Li, Mengya Si, and Fang Jiang
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0301 basic medicine ,Chemokine ,medicine.medical_treatment ,Central nervous system ,U937 ,Matrix metalloproteinase ,Blood–brain barrier ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,hemic and lymphatic diseases ,medicine ,cytokine ,CNS leukemia ,Original Research ,biology ,business.industry ,chemokine ,medicine.disease ,IL-60 ,Leukemia ,Vascular endothelial growth factor A ,030104 developmental biology ,medicine.anatomical_structure ,Cytokine ,Oncology ,Cancer Management and Research ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,business - Abstract
Mengya Si,1 Xiaoyang Jiao,2 Yazhen Li,2 Huanzhu Chen,2 Ping He,2 Fang Jiang1 1The First Affiliated Hospital of Shantou University Medical College, 2Cell Biology and Genetics Department, Shantou University Medical College, Shantou, People’s Republic of China Aim: Central nervous system (CNS) metastasis is a major obstacle in the treatment of leukemia, and the underlying mechanisms of leukemia CNS metastasis are not fully understood. The present study is an investigation of the role of the CNS microenvironment in leukemia CNS metastasis.Methods: Analog blood–brain barrier (BBB) was set by coculturing human brain microvascular endothelial cells (HBMVECs) and leukemia cells (U937 and IL-60), as well as HBMVECs and sera from leukemia patients, in vitro. The permeability of the HBMVEC monolayer and the levels of tight junction proteins, cytokines and chemokines (C&Ckines) were measured.Results: The permeability of HBMVECs increased when cocultured with leukemia sera. The expression of C&Ckines was significantly upregulated in HBMVECs cocultured with leukemia sera or leukemia cells, compared to the normal sera (P
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- 2018
5. Viral etiology, clinical and laboratory features of adult hemophagocytic lymphohistiocytosis
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Mengya Si, Zhi-chen Fan, Jinghong Chen, Yazhen Li, Ping He, Xuehua Wang, Xiaolan Chang, Xiaoyang Jiao, and Qindong Xie
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Secondary Hemophagocytic Lymphohistiocytosis ,Hemophagocytic lymphohistiocytosis ,biology ,business.industry ,Aspartate transaminase ,Disease ,medicine.disease ,Virology ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Alanine transaminase ,030220 oncology & carcinogenesis ,Immunology ,biology.protein ,Etiology ,Medicine ,030212 general & internal medicine ,Young adult ,business ,Epstein–Barr virus infection - Abstract
Secondary hemophagocytic lymphohistiocytosis (SHLH) is a potentially fatal hyperinflammatory syndrome with a heterogeneous etiology and has nonspecific clinical and laboratory findings. The diagnosis and treatment of adult SHLH is challenging because the etiology of the disease is difficult to identify, and the majority of reported cases are pediatric patients. The aim of this study was to describe the etiology, clinical characteristics, and outcomes of adult SHLH. Fifty-four adult patients who fulfilled the criteria of SHLH were enrolled in the study. Viral etiology, blood biomarkers, and clinical manifestations of SHLH were analyzed in these patients. Twenty-four SHLH patients had viraemia, whereas 30 SHLH patients were secondary to other diseases. Epstein-Barr virus (EBV) was the most common virus that associated SHLH among all viruses studied. Severe SHLH patients with EBV-viraemia presented significantly high levels of ferritin, lactate dehydrogenase, aspartate transaminase (AST), and alanine transaminase (ALT). Positively relationships existed between EBV DNA titers and levels of AST and ALT (P
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- 2015
6. Circulating microRNAs as a biomarker to predict therapy efficacy in hepatitis C patients with different genotypes
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Zhicheng Fan, Xiaoyang Jiao, Huanzhu Chen, Ping He, Yazhen Li, Mengya Si, and Qiaoxin Zhang
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0301 basic medicine ,Adult ,Male ,Genotype ,Hepatitis C virus ,Hepacivirus ,medicine.disease_cause ,Exosomes ,Microbiology ,03 medical and health sciences ,Young Adult ,medicine ,MiR-122 ,Humans ,Circulating MicroRNA ,Aged ,Aged, 80 and over ,biology ,business.industry ,Coinfection ,Hepatitis C ,Middle Aged ,Viral Load ,biology.organism_classification ,medicine.disease ,MicroRNAs ,030104 developmental biology ,Infectious Diseases ,Treatment Outcome ,Immunology ,Biomarker (medicine) ,RNA, Viral ,Female ,business ,Viral load ,Biomarkers - Abstract
Hepatitis C virus (HCV) genotype exerts a major influence on therapeutic response; however, the underlying mechanisms remain unclear. The aim of the study is to investigate the circulating microRNAs as the biomarkers to predict the response to therapy in chronic hepatitisC patients (HepC) with different genotypes.HepC patients were separated into 4 groups by genotype, healthy individuals were enrolled as the control. microRNA-122 (miR-122), microRNA-155 (miR-155) and HCV RNA in serum and exosome were measured, associations between microRNAs, viral load and other conventional biomarkers were analyzed.Serum and exosomal HCV RNA in genotype 6a group was highest, followed by genotype 3a/2a, and in genotype 1b were the lowest. The significant correlations existed between exosomal HCV RNA and serum HCVRNA. MiR-122, both in serum (miR-122ser) and in exosome (miR-122exo), was higher in normal control than in HCV group. Specifically, miR-122exo were significantly higher in genotype 1b than other genotype groups (p 0.05). On the contrary, miR-155exowas significantly lower in genotype 1b than in other groups (p 0.05 for both). A strongly positive association was found between miR-122/155 and HCV viral load in patients with various genotypes. Higher miR-122ser at the start of therapy predicts a better outcome.Expression of miR-122/155 differ in each genotypes, miR-122ser could be independent factor affecting the therapy efficacy, which had higher diagnostic value in predicting HCV outcome.
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- 2017
7. The Role of Circulating Tight Junction Proteins in Evaluating Blood Brain Barrier Disruption following Intracranial Hemorrhage
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Qing-Dong Xie, Dong-Yang Huang, Ya-zhen Li, Mengya Si, Zhicheng Fan, Xiaoyang Jiao, Ping He, and Xiaolan Chang
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Article Subject ,Clinical Biochemistry ,Blood–brain barrier ,Occludin ,chemistry.chemical_compound ,Cerebrospinal fluid ,White blood cell ,Genetics ,medicine ,Humans ,cardiovascular diseases ,Child ,Molecular Biology ,lcsh:R5-920 ,Tight Junction Proteins ,Tight junction ,business.industry ,Biochemistry (medical) ,Case-control study ,Albumin ,General Medicine ,Middle Aged ,nervous system diseases ,Vascular endothelial growth factor ,medicine.anatomical_structure ,chemistry ,Blood-Brain Barrier ,Case-Control Studies ,Child, Preschool ,Female ,business ,lcsh:Medicine (General) ,Intracranial Hemorrhages ,Biomarkers ,Research Article - Abstract
Brain injury after intracranial hemorrhage (ICH) results in significant morbidity and mortality. Blood brain barrier (BBB) disruption is a hallmark of ICH-induced brain injury; however, data mirroring BBB disruption in human ICH are scarce. The aim of this study was to assess the significance of circulating biomarkers in evaluating BBB disruption after ICH. Twenty-two patients with ICH were recruited in this study. Concentrations of the tight junction proteins (TJs) Claudin-5 (CLDN5), Occludin (OCLN), and zonula occludens 1 (ZO-1) and vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were measured by using enzyme-linked immunosorbent assay in serum and cerebrospinal fluid (CSF) samples obtained from patients with ICH. The white blood cell (WBC) count in blood and CSF, albumin (ALB) levels in the CSF (ALBCSF), and the BBB ratio were significantly higher in the ICH than in controls (p<0.05). Significantly higher levels of CLDN5, OCLN, ZO-1, MMP-9, and VEGF in CSF were observed in the ICH group; these biomarkers were also positively associated with BBB ratio (p<0.05). Our data revealed that circulating TJs could be considered the potential biomarkers reflecting the integrity of the BBB in ICH.
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- 2015
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8. The prognostic significance of serum and cerebrospinal fluid MMP-9, CCL2 and sVCAM-1 in leukemia CNS metastasis
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Ya-zhen Li, Zhicheng Fan, Qing-Dong Xie, Mengya Si, Xiaoyang Jiao, and Xiao-Lan Chang
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Adult ,Male ,Cancer Research ,Chemokine ,Pathology ,medicine.medical_specialty ,Neurology ,Adolescent ,Central nervous system ,Vascular Cell Adhesion Molecule-1 ,Kaplan-Meier Estimate ,CCL2 ,Matrix metalloproteinase ,Gastroenterology ,Sensitivity and Specificity ,Metastasis ,Central Nervous System Neoplasms ,Young Adult ,Cerebrospinal fluid ,Risk Factors ,Internal medicine ,Biomarkers, Tumor ,Medicine ,Humans ,Chemokine CCL2 ,Principal Component Analysis ,Leukemia ,biology ,business.industry ,medicine.disease ,Prognosis ,medicine.anatomical_structure ,Oncology ,Matrix Metalloproteinase 9 ,ROC Curve ,biology.protein ,Female ,Neurology (clinical) ,business - Abstract
Metastasis to the central nervous system (CNS) is the primary obstacle in leukemia treatment. Matrix metalloproteinase-9 (MMP-9), chemokine ligand-2 (CCL2) and soluble vascular adhesion molecule-1 (sVCAM-1) play crucial roles in tumor cell adhesion, motivation and survival, but their roles in leukemia CNS metastasis remain to be elucidated. We investigated the prognostic significance of serum and cerebrospinal fluid (CSF) MMP-9, CCL2 and sVCAM-1 in leukemia patients to explore their potential as predictive biomarkers of the development of CNS leukemia (CNSL). MMP-9, CCL2 and sVCAM-1 were measured in paired CSF and serum samples collecting from 33 leukemia patients with or without CNS metastasis. Other risk factors related to CNSL prognosis were also analyzed. sVCAM-1Serum and CCL2Serum/CSF were significantly higher in the CNSL group than in the non-CNSL group and the controls (p0.05). MMP-9Serum was insignificantly lower in the CNSL group than in the non-CNSL group and the controls (p0.05). No differences were found for the sVCAM-1Serum, CCL2Serum, and MMP-9Serum levels between non-CNSL patients and controls (p0.05). MMP-9CSF was significantly higher in the CNSL group than both the non-CNSL and the control groups (p0.05). The indexes of sVCAM-1, CCL2, and MMP-9 in the CNSL group were lower than in the controls (p0.05). Positive correlations were determined between the MMP-9CSF and the ALBCSF/BBB value/WBCCSF, between sVCAM-1Serum and the WBCCSF/BBB value. Negative correlations existed between MMP-9Serum and the ALBCSF/BBB value/WBCCSF, and between the CCL2 index and ALBCSF. sVCAM-1Serum was positively associated with event-free survival (EFS), and patients with higher levels of ALBCSF, MMP-9CSF/Serum, CCL2CSF/Serum, and sVCAM-1CSF/Serum had shorter EFS. MMP-9CSF, CCL2CSF and sVCAM-1CSF are the first three principal components analyzed by cluster and principal component analysis. Our data suggest that MMP-9, CCL2 and sVCAM-1 in the CSF may be more potent than serum in predicting the possibility of leukemia metastatic CNS and the outcome of CNSL patients.
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- 2014
9. The soluble VEGF receptor 1 and 2 expression in cerebral spinal fluid as an indicator for leukemia central nervous system metastasis
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Yue-Ting Tang, Li Guo, Mengya Si, Fang Jiang, and Xiaoyang Jiao
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Neurology ,Adolescent ,Central nervous system ,Enzyme-Linked Immunosorbent Assay ,Kaplan-Meier Estimate ,Biology ,Disease-Free Survival ,Metastasis ,Central Nervous System Neoplasms ,Young Adult ,Cerebrospinal fluid ,Cell surface receptor ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,Child ,Leukemia ,Vascular Endothelial Growth Factor Receptor-1 ,Proportional hazards model ,medicine.disease ,Prognosis ,Vascular Endothelial Growth Factor Receptor-2 ,Vascular endothelial growth factor A ,Endocrinology ,medicine.anatomical_structure ,Oncology ,Child, Preschool ,Cancer research ,Female ,Neurology (clinical) - Abstract
Over-expression of vascular endothelial growth factor A (VEGF-A) is correlated with leukemia metastasis. VEGF-A acts by binding to its membrane receptors R1 and R2 present in soluble forms (sVEGFR1, sVEGFR2) with different functions. sVEGFR could inhibit VEGF-A bioactivities, associated with favorable prognosis in solid tumors. However, its role is obscure in central nervous system leukemia (CNSL). The aim of this study was to investigate sVEGFR1, R2 as biomarkers in CNSL. Paired cerebrospinal fluid (CSF) and serum samples were collected from 35 leukemia cases with or without CNS metastasis. Levels of sVEGFR1 and sVEGFR2 in both CSF (sVEGFR1CSF, sVEGFR2CSF) and serum (sVEGFR1Serum, sVEGFR2Serum) were detected by ELISA. Other risk factors related to CNSL prognosis were also analyzed. sVEGFRSerum levels were 2.54-fold (sVEGFR1) and 25.6-fold (sVEGFR2) higher than sVEGFRCSF in both leukemic groups. sVEGFR1CSF in CNSL were 33 % higher than in the non-CNSL, and the levels of sVEGFR2CSF and sVEGFR2Serum had the same trend. Elevated sVEGFR1CSF and sVEGFR2CSF is closely correlated with blood-brain barrier (BBB) values and WBCCSF that is an indicator of CNSL disease burden. Cox regression analysis showed that the sVEGFR2CSF had a positive effect on event-free survival. Our data suggest that sVEGFR2CSF may be more potent than sVEGFR1CSF in predicting the outcome of leukemia patients, the balance between sVEGFR2CSF and VEGF-ACSF levels might be crucial for the progression of CNSL.
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- 2012
10. Expression and significance of vascular endothelial growth factor A and C in leukemia central nervous system metastasis
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Yue-Ting Tang, Li Guo, Xiaoyang Jiao, Fang Jiang, and Mengya Si
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Adult ,Male ,Vascular Endothelial Growth Factor A ,Cancer Research ,Adolescent ,Central nervous system ,Vascular Endothelial Growth Factor C ,Blood–brain barrier ,Metastasis ,Pathogenesis ,Central Nervous System Neoplasms ,Young Adult ,Cerebrospinal fluid ,Neoplasms ,Medicine ,Humans ,Neoplasm Metastasis ,Child ,business.industry ,Case-control study ,Hematology ,Middle Aged ,medicine.disease ,Leukemia ,Vascular endothelial growth factor A ,medicine.anatomical_structure ,Oncology ,Blood-Brain Barrier ,Case-Control Studies ,Immunology ,Multivariate Analysis ,Female ,business - Abstract
Metastasis to the central nervous system (CNS) is an obstacle for leukemia treatment, the mechanisms of which remain to be elucidated. VEGF-A and VEGF-C are suspected to participate in this process. Paired of cerebrospinal fluid (CSF) and serum samples were collected from leukemia and control cases. Levels of VEGF-A and VEGF-C in both CSF (VEGF-ACSF, VEGF-CCSF) and serum (VEGF-ASerum, VEGF-CSerum) were detected by ELISA. Our data show that higher levels of VEGF-ACSF are closely related to CNS leukemia (CNSL), and VEGF-ACSF may be a better predictor than the other risk factors elucidating the pathogenesis and development of CNSL.
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- 2012
11. The Role of Circulating Tight Junction Proteins in Evaluating Blood Brain Barrier Disruption following Intracranial Hemorrhage.
- Author
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Xiaoyang Jiao, Ping He, Yazhen Li, Zhicheng Fan, Mengya Si, Qingdong Xie, Xiaolan Chang, and Dongyang Huang
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TIGHT junctions ,BLOOD-brain barrier ,BRAIN injuries ,INTRACRANIAL hematoma ,MATRIX metalloproteinases ,VASCULAR endothelial growth factors - Abstract
Brain injury after intracranial hemorrhage (ICH) results in significant morbidity and mortality. Blood brain barrier (BBB) disruption is a hallmark of ICH-induced brain injury; however, data mirroring BBB disruption in human ICH are scarce. The aim of this study was to assess the significance of circulating biomarkers in evaluating BBB disruption after ICH. Twenty-two patients with ICH were recruited in this study. Concentrations of the tight junction proteins (TJs) Claudin-5 (CLDN5), Occludin (OCLN), and zonula occludens 1 (ZO-1) and vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were measured by using enzyme-linked immunosorbent assay in serum and cerebrospinal fluid (CSF) samples obtained from patients with ICH. The white blood cell (WBC) count in blood and CSF, albumin (ALB) levels in the CSF (ALBCSF), and the BBB ratio were significantly higher in the ICH than in controls (p < 0.05). Significantly higher levels of CLDN5, OCLN, ZO-1, MMP-9, and VEGF in CSF were observed in the ICH group; these biomarkers were also positively associated with BBB ratio (p < 0.05). Our data revealed that circulating TJs could be considered the potential biomarkers reflecting the integrity of the BBB in ICH. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
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