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Drought stress limits wheat production around the world. Here, the authors report allelic variation of a WD40 encoding gene TaWD40-4B.1 contributes to wheat drought tolerance by interacting with canonical catalases as a chaperone to promote their activities to avoid ROS over accumulation.

Mixed-flow pumps have been extensively employed in daily life, improving their energy characteristics contribute to the reduction of energy consumption worldwide. In this study, to overcome the decrease of optimization upper limit caused by using a single type of parameter as the design parameter, a typical mixed-flow pump was chosen for study, and its impeller was parameterized by five geometric and eight hydrodynamic parameters. With head and efficiency as the constraint and optimization objective respectively, 27 schemes were constructed by the Taguchi method. The influence of design factors to the objective and constraint was analyzed based on range and regression analysis. The optimization mechanism was elucidated using the entropy production method. The result reveals that the geometric and hydrodynamic parameters have a significantly impact on the mixed-flow pump’s energy characteristics. The optimized model head is 12.43m, which meets the constraints, while the efficiency increases by 3.2%–88.51%. Therefore, considering both geometric and hydrodynamic parameters in the mixed-flow pump optimization is workable and necessary. This paper can provide practical instructions on the optimal design of different turbomachines.

In this study, a comparative analysis of the shutdown transitions of a full-flow pump and an axial-flow pump was carried out through numerical simulation and model tests. The UDF method was used to achieve control of the impeller rotational speed during shutdown. The results show that during the shutdown transition, the rate of decline of rotational speed, flow rate, and torque of the axial-flow pump are greater than those of the full-flow pump, so the axial-flow pump stops faster than the full-flow pump. The axial force of the axial-flow pump is significantly lower than that of the full-flow pump, and the maximum value of the radial force of the axial-flow pump is approximately 1.14 times that of the full-flow pump. Due to the influence of the clearance backflow vortex, the impeller inlet and outlet of the full-flow pump generate clearance backflow vortices in the near-wall area, resulting in the overall flow pattern of the impeller chamber being worse than that of the axial-flow pump and the hydraulic loss being greater than that of the axial-flow pump. The runaway speed and flow rate of the axial-flow pump are higher than those of the full-flow pump. Due to the influence of the clearance backflow, the range of the high entropy production rate at the suction side of the impeller of the full-flow pump is always larger than that of the axial-flow pump. The research results in this paper can provide theoretical support for the selection and operation of pumps in large low-head pumping stations.

Turbomachinery optimization based on the inverse design method (IDM) has been investigated in several previous studies, however, due to head constraints, most of these studies have adopted the constant impeller outlet angular momentum (IOAM) distribution, namely the free vortex design, which in turn reduces the optimization effect. To overcome these drawbacks, forced and compound vortex designs are proposed here by parameterizing the IOAM using a parabola, and the effects of different vortex designs on the optimization results of a mixed-flow pump impeller are investigated. First, a baseline mixed-flow pump is simulated and experimentally verified. Second, based on the IDM, the impeller is parameterized for the three vortex designs. Finally, it is optimized by artificial neural network and genetic algorithm to maximize the weighted efficiency at 0.8Qdes, 1.0Qdes and 1.2Qdes, and the results are analyzed. The results show that the weighted efficiency of the forced and compound vortex design is improved by 1.33% and 1.69% respectively compared to free vortex design. The internal flow analysis reveals that the improved efficiency of the compound vortex design can be attributed to the improved impeller-outlet flow regime. Finally, the energy characteristics of the preferred and baseline models are compared using the entropy production method.

Efficient antioxidant enzymatic system contributes to salt tolerance of plants via avoiding ROS over-accumulation. Peroxiredoxins are crucial components of the reactive oxygen species (ROS) scavenging machinery in plant cells, but whether they offer salt tolerance with potential for germplasm improvement has not been well addressed in wheat. In this work, we confirmed the role of a wheat 2-Cys peroxiredoxin gene TaBAS1 that was identified through the proteomic analysis. TaBAS1 overexpression enhanced the salt tolerance of wheat at both germination and seedling stages. TaBAS1 overexpression enhanced the tolerance to oxidative stress, promoted the activities of ROS scavenging enzymes, and reduced ROS accumulation under salt stress. TaBAS1 overexpression promoted the activity of ROS production associated NADPH oxidase, and the inhibition of NADPH oxidase activity abolished the role of TaBAS1 in salt and oxidative tolerance. Moreover, the inhibition of NADPH-thioredoxin reductase C activity erased the performance of TaBAS1 in the tolerance to salt and oxidative stress. The ectopic expression of TaBAS1 in Arabidopsis exhibited the same performance, showing the conserved role of 2-Cys peroxiredoxins in salt tolerance in plants. TaBAS1 overexpression enhanced the grain yield of wheat under salt stress but not the control condition, not imposing the trade-offs between yield and tolerance. Thus, TaBAS1 could be used for molecular breeding of wheat with superior salt tolerance.

The diploidization of polyploid genomes is accompanied by genomic variation, including synonymous nucleotide substitutions that may lead to synonymous codon usage bias (SCUB). SCUB can mirror the evolutionary specialization of plants, but its effect on the formation of polyploidies is not well documented. We explored this issue here with hexaploid wheat and its progenitors. Synonymous codons (SCs) ending in either cytosine (NNC) or guanidine (NNG) were more frequent than those ending in either adenosine (NNA) or thymine (NNT), and the preference for NNC/G codons followed the increase in genome ploidy. The ratios between NNC/G and NNA/T codons gradually decreased in genes with more introns, and the difference in these ratios between wheat and its progenitors diminished with increasing ploidy. SCUB frequencies were heterogeneous among exons, and the bias preferred to NNA/T in more internal exons, especially for genes with more exons; while the preference did not appear to associate with ploidy. The SCUB alteration of the progenitors was different during the formation of hexaploid wheat, so that SCUB was the homogeneous among A, B and D subgenomes. DNA methylation-mediated conversion from cytosine to thymine weakened following the increase of genome ploidy, coinciding with the stronger bias for NNC/G SCs in the genome as a function of ploidy, suggesting that SCUB contribute to the epigenetic variation in hexaploid wheat. The patterns in SCUB mirrored the formation of hexaploid wheat, which provides new insight into genome shock-induced genetic variation during polyploidization. SCs representing non-neutral synonymous mutations can be used for genetic dissection and improvement of agricultural traits of wheat and other polyploidies.

In this study, model tests and numerical simulations are conducted to study the bi-directional full-flow pump (BFFP). Firstly, the head, efficiency and shaft power of the BFFP are significantly higher in the positive operating condition than in the negative operating condition. When the unit operates in the positive direction, the clearance reflux flow rate, the flow uniformity and velocity-weighted average angle of the impeller inlet, and the intensity of pressure pulsation are significantly greater than those during the negative operation. When the pump unit is operating at low flow rates, the clearance reflux produces a significant disturbance to the impeller inlet main flow. Two vortices appear in the near-wall area of the clearance outlet (i.e., impeller inlet), and the range of vortices is larger in the positive operation than in the negative operation. Secondly, at low-flow and design-flow conditions, the total entropy production of the pump unit in the positive direction is greater than that in the negative direction. When at small- and design-flow rates, the amplitude of pressure pulsation in the positive direction is smaller than that in the negative direction. This study will contribute to the research and development of a full-flow pump.

As an effective measure to solve the dilemma of urban energy consumption and economic development, the new energy demonstration city (NEDC) policy in China could greatly promote the development of the new energy industry and urban economy. This study aims to explore how the NEDC policy effectively promotes the growth of urban land use efficiency (ULUE), an essential indicator of economic development, through the urban industrial structure. Based on the panel data of 285 Chinese cities during 2003–2019, this study took the NEDC policy as a quasi-natural experiment and employed the PSM-DID method and the mediating effect model to objectively evaluate its policy effects. We found that the NEDC policy could significantly promote the growth of the ULUE. Specifically, the ULUE has been significantly improved by 17.0%. The NEDC policy could also promote the ULUE indirectly through the mediating effect of industrial structure advancement (ISA), but the mediating effect of industrial structure rationalization (ISR) was not significant. Furthermore, the promotional effect of the NEDC policy on the ULUE has regional heterogeneity. Compared with eastern cities and high-innovation cities, central and western cities and medium-innovation and low-innovation cities can obtain much higher promotion effects. This study may provide some policy inspiration for policymakers to support low-corban and sustainable economic development and urban land use.

Although the importance of inclusion has been firmly supported by prior studies, the question of whether certain subgroups exist in the workplace whose members feel more or less included remains under-explored, limiting our understanding of how an inclusive workplace can be achieved. To address this gap, the current study conducted a latent profile analysis (LPA) to obtain evidence-based information regarding employees’ sense of inclusion in their organizations. Using a sample of 1,168 participants engaged in multiple industries in China, we identified three profiles of inclusion, with a largest proportion feeling a moderate level of inclusion (69.5%), a smaller proportion feeling a high level of inclusion (22.7%), and a tiny proportion feeling a low level of inclusion (7.8%). The three profiles differ significantly on key variables, demonstrating that the group feeling more included tends to be more aged and tenured, hold a higher educational degree, work in the high-tech sector, and come from a developed area. Such a group also shows more engagement in their work but less exhaustion, feels that they have more opportunities for development, and gains more support from colleagues and supervisors. Our findings point to the existence of subgroups of inclusion within the Chinese context and highlight the characteristics of these profiles, which in turn shed lights on how we can reach the goal of inclusion.

p62/SQSTM1 (sequestosome-1) is a key protein involved in multiple cellular bioprocesses including autophagy, nutrient sensing, cell growth, cell death, and survival. Therefore, it is implicated in human diseases such as obesity and cancer. Here, we show that the CUL5–ASB6 complex is a ubiquitin E3 ligase complex mediating p62 ubiquitination and degradation. Depletion of CUL5 or ASB6 induced p62 accumulation, and overexpression of ASB6 promoted ubiquitination and degradation of p62. Functionally, ASB6 overexpression can inhibit the proliferation of MEF and hepatocellular carcinoma cells by reducing p62 protein level, and impair the occurrence of autophagy. Overall, our study identified a new molecular mechanism regulating p62 stability, which may provide additional insights for understanding the delicate control of p62 and cell proliferation–autophagy control in physiological and pathological settings.

Asymmetric somatic hybridization is an efficient strategy for crop breeding by introducing exogenous chromatin fragments, which leads to whole genomic shock and local chromosomal shock that induces genome-wide genetic variation including indel (insertion and deletion) and nucleotide substitution. Nucleotide substitution causes synonymous codon usage bias (SCUB), an indicator of genomic mutation and natural selection. However, how asymmetric somatic hybridization affects SCUB has not been addressed. Here, we explored this issue by comparing expressed sequence tags of a common wheat cultivar and its asymmetric somatic hybrid line. Asymmetric somatic hybridization affected SCUB and promoted the bias to A- and T-ending synonymous codon (SCs). SCUB frequencies in chromosomes introgressed with exogenous fragments were comparable to those in chromosomes without exogenous fragments, showing that exogenous fragments had no local chromosomal effect. Asymmetric somatic hybridization affected SCUB frequencies in indel-flanking sequences more strongly than in non-flanking sequences, and this stronger effect was present in both chromosomes with and without exogenous fragments. DNA methylation-driven SCUB shift was more pronounced than other SC pairs. SCUB shift was similar among seven groups of allelic chromosomes as well as three sub-genomes. Our work demonstrates that the SCUB shift induced by asymmetric somatic hybridization is attributed to the whole genomic shock, and DNA methylation is a putative force of SCUB shift during asymmetric somatic hybridization. Asymmetric somatic hybridization provides an available method for deepening the nature of SCUB shift and genetic variation induced by genomic shock.

The performance of energy recovery turbine (ERT) directly determines the cost and energy consumption of reverse osmosis desalination. In order to study the performance and loss mechanisms of ERT under different conditions, the external characteristics and the losses of different components were quantitatively analyzed. The loss mechanisms of each component in the turbine were revealed through the comparative analysis of the internal flow field. The results show that the efficiency is 2.2% higher than that at the design speed when turbine runs at n = 22000 r/min. The impeller losses account for more than 67% of the total losses. The impeller loss is mainly observed at the leading edge. The vortex on the pressure side of the leading edge is caused by the impact effect, while the vortex on the suction side of the leading edge is caused by the flow separation. With the increase in the rotating speed, the loss caused by flow separation in impeller decreases obviously. The volute loss is mainly observed near the tongue, which is caused by the flow separation at the tongue. The design of the tongue is very important to the performance of the volute. The turbulent kinetic energy (TKE) and loss decrease with the increase in the rotating speed. The loss in the draft tube is mainly observed at the inlet core. With the increase in the rotating speed, the turbulence pulsation and the radial pressure fluctuation amplitude reduce. Therefore, the turbine can be operated at the design or slightly higher than the design rotating speed under the condition that both the hydraulic condition and the intensity are satisfied, which are conducive to the efficient utilization of energy.

Synonymous codon usage bias (SCUB) of both nuclear and organellar genes can mirror the evolutionary specialization of plants. The polyploidization process exposes the nucleus to genomic shock, a syndrome which promotes, among other genetic variants, SCUB. Its effect on organellar genes has not, however, been widely addressed. The present analysis targeted the chloroplast genomes of two leading polyploid crop species, namely cotton and bread wheat. The frequency of codons in the chloroplast genomes ending in either adenosine (NNA) or thymine (NNT) proved to be higher than those ending in either guanidine or cytosine (NNG or NNC), and this difference was conserved when comparisons were made between polyploid and diploid forms in both the cotton and wheat taxa. Preference for NNA/T codons was heterogeneous among genes with various numbers of introns and was also differential among the exons. SCUB patterns distinguished tetraploid cotton from its diploid progenitor species, as well as bread wheat from its diploid/tetraploid progenitor species, indicating that SCUB in the chloroplast genome partially mirrors the formation of polyploidies.

Abstract Background Asymmetric somatic hybridization is an efficient crop breeding approach by introducing several exogenous chromatin fragments, which leads to genomic shock and therefore induces genome-wide genetic variation. However, the fundamental question concerning the genetic variation such as whether it occurs randomly and suffers from selection pressure remains unknown. Results Here, we explored this issue by comparing expressed sequence tags of a common wheat cultivar and its asymmetric somatic hybrid line. Both nucleotide substitutions and indels (insertions and deletions) had lower frequencies in coding sequences than in un-translated regions. The frequencies of nucleotide substitutions and indels were both comparable between chromosomes with and without introgressed fragments. Nucleotide substitutions distributed unevenly and were preferential to indel-flanking sequences, and the frequency of nucleotide substitutions at 5′-flanking sequences of indels was obviously higher in chromosomes with introgressed fragments than in those without exogenous fragment. Nucleotide substitutions and indels both had various frequencies among seven groups of allelic chromosomes, and the frequencies of nucleotide substitutions were strongly negatively correlative to those of indels. Among three sets of genomes, the frequencies of nucleotide substitutions and indels were both heterogeneous, and the frequencies of nucleotide substitutions exhibited drastically positive correlation to those of indels. Conclusions Our work demonstrates that the genetic variation induced by asymmetric somatic hybridization is attributed to both whole genomic shock and local chromosomal shock, which is a predetermined and non-random genetic event being closely associated with selection pressure. Asymmetric somatic hybrids provide a worthwhile model to further investigate the nature of genomic shock induced genetic variation.

The development of social production and the agglomeration of the urban population have brought tremendous pressure to transportation infrastructure. However, the impacts of transportation development on urban land use systems have not been well investigated. Under the pressure of limited land resources, the impact of transportation infrastructure on urban land use efficiency (ULUE) is receiving increasing attention from scholars and needs to be explored. By collecting panel data from 30 regions in China from 2003 to 2018, in this study we constructed a spatial Durbin model and a panel threshold regression model to explore the spatial spillover effects and threshold effects of transportation infrastructure on ULUE. The most obvious findings emerging from this study are that (1) ULUE is not randomly distributed over different regions in China, but has an obvious positive spatial correlation; (2) transportation infrastructure has significant positive direct and spatial spillover effects on ULUE and the direct effects of transportation infrastructure (0.823) are significantly stronger than the spatial spillover effects (0.263); (3) the impact of transportation infrastructure on ULUE has a significant double threshold effect, and the threshold values are 4.520 and 6.429 respectively, and with the improvement of transportation infrastructure, its marginal effects on ULUE show a downward trend. This paper provides theoretical support for policymakers to achieve cross-regional cooperation on land use and transportation infrastructure construction and inspirations for sustainable development.

Through Silicon Via (TSV) technology is capable meeting effective, compact, high density, high integration, and high-performance requirements. In high-frequency applications, with the rapid development of 5G and millimeter-wave radar, the TSV interposer will become a competitive choice for radio frequency system-in-package (RF SIP) substrates. This paper presents a redundant TSV interconnect design for high resistivity Si interposers for millimeter-wave applications. To verify its feasibility, a set of test structures capable of working at millimeter waves are designed, which are composed of three pieces of CPW (coplanar waveguide) lines connected by single TSV, dual redundant TSV, and quad redundant TSV interconnects. First, HFSS software is used for modeling and simulation, then, a modified equivalent circuit model is established to analysis the effect of the redundant TSVs on the high-frequency transmission performance to solidify the HFSS based simulation. At the same time, a failure simulation was carried out and results prove that redundant TSV can still work normally at 44 GHz frequency when failure occurs. Using the developed TSV process, the sample is then fabricated and tested. Using L-2L de-embedding method to extract S-parameters of the TSV interconnection. The insertion loss of dual and quad redundant TSVs are 0.19 dB and 0.46 dB at 40 GHz, respectively.

The spanwise distribution of impeller exit circulation (SDIEC) has an important influence on the performance of the impeller. To quantitatively study the influence of SDIEC on optimization results, a comprehensive optimization system composed of the computational fluid dynamics, inverse design method, design of experiment, surrogate model, and optimization algorithm was used to optimize a mixed flow pump impeller in two different cases. In the first case, the influence of SDIEC was ignored, while in the second case, the influence of SDIEC was considered. The result shows that the optimization upper limit can be further improved when the influence of SDIEC is considered in the optimization process. The pump efficiency of the preferred optimized impeller F1 obtained in the first case at 1.2Qdes, 1.0Qdes, and 0.8Qdes are increased by 6.48%, 2.41%, and 0.06%, respectively, over the baseline model. Moreover, the pump efficiency of the preferred optimized impeller S2 obtained in the second case further increased by 0.76%, 1.24%, and 1.21%, respectively, over impeller F1. Furthermore, the influence of SDIEC on the performance of the mixed flow pump is clarified by a comparative analysis of the internal flow field.

Drug related cue-induced reactivity plays a significant role in maintaining drug use and relapse in addicted individuals. The activation of Dorsolateral striatum-Sensorimotor system (DLS-SM) has been suggested as an important route through which drug cues may induce automatic drug using behavior. The current study used fMRI to investigate the reactivity of heroin abstinent individuals to different types of cues, to clarify the characteristics of the cues that induce the activation of the sensorimotor area. Forty heroin-dependent abstinent individuals and 29 healthy subjects were recruited to perform the heroin cue-reactivity task during fMRI. The participants’ subjective craving and physical signs were evaluated before and after scanning. Whole-brain analysis showed that compared to drug use tool and drug cues, cues related to drug use action were more likely to activate posterior central gyrus, para-hippocampus, supra marginal gyrus, superior parietal lobule (SPL) and inferior parietal lobule (IPL). These areas are involved in motor preparation and output, indicating that the sensorimotor area is also an important neural basis of craving and automatic drug using behavior, and may mediate craving and drug seeking behavior. Our findings thus suggest that cues related to drug using action may induce automatic drug seeking behavior more than cues related only to the drug itself.

Synonymous codon usage bias (SCUB) is a common event that a non-uniform usage of codons often occurs in nearly all organisms. We previously found that SCUB is correlated with both intron number and exon position in the plant nuclear genome but not in the plastid genome; SCUB in both nuclear and plastid genome can mirror the evolutionary specialization. However, how about the rules in the mitochondrial genome has not been addressed. Here, we present an analysis of SCUB in the mitochondrial genome, based on 24 plant species ranging from algae to land plants. The frequencies of NNA and NNT (A- and T-ending codons) are higher than those of NNG and NNC, with the strongest preference in bryophytes and the weakest in land plants, suggesting an association between SCUB and plant evolution. The preference for NNA and NNT is more evident in genes harboring a greater number of introns in land plants, but the bias to NNA and NNT exhibits even among exons. The pattern of SCUB in the mitochondrial genome differs in some respects to that present in both the nuclear and plastid genomes.

Leaf type is an important trait that closely associates with crop yield. WRKY transcription factors exert diverse regulatory effects in plants, but their roles in the determination of leaf type have not been reported so far. In this work, we isolated a WRKY transcription factor gene TaWRKY71-1 from a wheat introgression line SR3, which has larger leaves, superior growth capacity and higher yield than its parent common wheat JN177. TaWRKY71-1 specifically expressed in leaves, and produced more mRNA in SR3 than in JN177. TaWRKY71-1 localized in the nucleus and had no transcriptional activation activity. TaWRKY71-1 overexpression in Arabidopsis resulted in hyponastic rosette leaves, and the hyponastic strength was closely correlative with the transcription level of the transgene. The spongy mesophyll cells at abaxial side of leaves were drastically compacted by TaWRKY71-1 overexpression. In TaWRKY71-1 overexpression Arabidopsis, the expression of IAMT1 that encodes a methyltransferase converting free indole-3-acetic acid (IAA) to methyl-IAA ester (MeIAA) to alter auxin homeostatic level was induced, and the induction level was dependent on the abundance of TaWRKY71-1 transcripts. Besides, several TCP genes that had found to be restricted by IAMT1 had lower expression levels as well. Our results suggest that TaWRKY71-1 causes hyponastic leaves through altering auxin homeostatic level by promoting the conversion of IAA to MeIAA.