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To investigate different irradiation doses of Se-enriched A.Auricularia polysaccharide (SAAP) in type 1 diabetes mellitus (T1DM) mice. In this study, extraction of polysaccharides from selenium-enriched fungus was carried out after 60Co-γ-ray irradiation treatment. The optimal extraction conditions of SAAP were optimized by response surface methodology. The optimal irradiation dose was selected for the study of in vivo hypoglycemia by comparing the hypoglycemic effect of SAAP under different irradiation doses. C57BL/6 T1DM mice model was established by injecting streptozotocin (STZ) to evaluate the effects of fasting blood glucose (FBG), oral glucose tolerance (OGTT), and SAAP on the glucolipid metabolism and the regulation of oxidative stress in T1DM mice. The results showed that after response surface optimization, the optimal extraction conditions for SAAP: Material-liquid ratio of 37.98:1 mL/g, extraction temperature of 97.94 ℃, extraction time of 3.42 h. The optimal parameters were as follows: liquid-material ratio of 38:1 mL/g, extraction temperature 98 ℃, extraction time of 3.5 h, and SAAP concentration of 0.73 mg/mL. SAAP could improve the symptoms of polydipsia, polyphagia and weight loss in T1DM mice (P

Abstract Jellyfish exhibit innovative swimming patterns that contribute to exploring the origins of animal locomotion. However, the genetic and cellular basis of these patterns remains unclear. Herein, we generated chromosome-level genome assemblies of two jellyfish species, Turritopsis rubra and Aurelia coerulea, which exhibit straight and free-swimming patterns, respectively. We observe positive selection of numerous genes involved in statolith formation, hair cell ciliogenesis, ciliary motility, and motor neuron function. The lineage-specific absence of otolith morphogenesis- and ciliary movement-related genes in T. rubra may be associated with homeostatic structural statocyst loss and straight swimming pattern. Notably, single-cell transcriptomic analyses covering key developmental stages reveal the enrichment of diapause-related genes in the cyst during reverse development, suggesting that the sustained diapause state favours the development of new polyps under favourable conditions. This study highlights the complex relationship between genetics, locomotion patterns and survival strategies in jellyfish, thereby providing valuable insights into the evolutionary lineages of movement and adaptation in the animal kingdom.

BackgroundWith the advancement of society, the cultivation of medical professionals equipped with solid theoretical knowledge, a strong sense of innovation, and critical thinking has become a crucial goal in the reform of medical higher education. Over recent years, the hybrid Problem-Based Learning (hPBL) model, a blend of Problem-Based Learning (PBL) and Lecture-Based Learning (LBL), has emerged as a novel approach in the medical education reform landscape of China. The application and efficacy of the hPBL model in medical experimental courses have piqued the interest of medical educators. The aim of this study was to appraise the application and effectiveness of the hPBL model in the experimental course of Medical Molecular Biology at Beihua University.MethodsUtilizing the “Rain Classroom” platform, students from the Preventive Medicine and Medical Imaging programs were allocated to either the hPBL or LBL method for their Medical Molecular Biology experimental courses. The hPBL model’s impact on students’ performance was evaluated across four domains: experimental theory, experimental operation, experimental report, and practical application. Questionnaires were employed to gauge students’ experiences and perceptions.ResultsThe results indicated that the final assessment scores of the hPBL group were significantly superior to those of the LBL group. Moreover, the hPBL model effectively amplified students’ self-learning capability, practical application skills, and communication competencies. Students expressed a high degree of satisfaction with this blended learning model.ConclusionThe hPBL model, which amalgamates PBL and LBL, has demonstrated its effectiveness in medical education. Its implementation in the experimental course of Medical Molecular Biology at Beihua University yielded positive outcomes, enhancing students’ performance and satisfaction levels. Consequently, it is recommended that the hPBL model be further promulgated in other medical experimental courses.

Porcine circovirus-associated diseases, caused by porcine circovirus type 2 (PCV2), are widespread and result in significant economic losses to the global swine industry. PCV2 can currently be divided into nine genotypes (PCV2a to PCV2i), with the currently dominant one being the PCV2d genotype. In this study, 2675 samples from 804 pig farms in 13 cities in Jiangsu Province, China, were collected between 2014 and 2021 and subjected to polymerase chain reaction analysis to investigate the frequency and genetic diversity of PCV2. The results showed that 41.42% (1108/2675) of samples tested positive for PCV2. The researchers further analyzed the genetic characteristics of 251 PCV2 strains and found that they belonged to the following four genotypes: PCV2a, PCV2b, PCV2d, and PCV2i. The dominant genotype was PCV2d, with a frequency of 49.80% (125/251). The detection rate of PCV2b was significantly higher than those of PCV2a and PCV2i, at 35.46% (89/251), 7.57% (19/251), and 7.17% (18/251), respectively. The percentage of different genotypes of PCV2 varied irregularly over time. We have further revealed the fingerprint of PCV2i genomic nucleotides for the first time. In conclusion, this study illustrates the high frequency and evolutionary features of PCV2 in Jiangsu Province over the past few years.

In recent years, ultra-large-scale offshore wind turbines have attracted widespread attention. However, accurately evaluating the motion responses of offshore wind turbines under extreme conditions, especially for semisubmersible floating off-shore wind turbines, is often challenging. In order to assess the operational behavior of wind turbines under wind and wave loads, this paper adopted a numerical analysis method to solve the motion responses under extreme conditions. It specifically examines the motion responses of the IEA 15 MW wind turbine in terms of surge, heave, and pitch direction, focusing on environmental loads that occur once every 50 years. The results show that the wind turbine can still operate normally under the Ultimate condition. However, the average amplitude increased by 7% in the pitch direction and decreased by 4% in the heave direction compared to the rated condition. Under extreme conditions (occurring once every 50 years), with the wind turbine parked, the average amplitude in the surge direction reduced by 33%, while the average amplitude in the pitch direction reduced by 106%. Thus, it is essential to pitch the blades and brake the generator in extreme environmental conditions to ensure the safety of the wind turbine.

Histo-blood group antigens (HBGAs) comprise a family of cell-surface carbohydrates that are considered norovirus-specific binding receptors or ligands. HBGA-like molecules have also been detected in oysters as common norovirus carriers, although the pathway involved in the synthesis of these molecules in oysters has yet to be elucidated. We isolated and identified a key gene involved in the synthesis of HBGA-like molecules, FUT1, from Crassostrea gigas, named CgFUT1. Real-time quantitative polymerase chain reaction analysis showed that CgFUT1 mRNA was expressed in the mantle, gill, muscle, labellum, and hepatopancreatic tissues of C. gigas, with the hepatopancreas exhibiting the highest expression level. A recombinant CgFUT1 protein with a molecular mass of 38.0 kDa was expressed in Escherichia coli using a prokaryotic expression vector. A eukaryotic expression plasmid was constructed and transfected into Chinese hamster ovary (CHO) cells. The expression of CgFUT1 and membrane localization of type H-2 HBGA-like molecules in CHO cells were detected using Western blotting and cellular immunofluorescence, respectively. This study indicated that CgFUT1, expressed in C. gigas tissues, can synthesize type H-2 HBGA-like molecules. This finding provides a new perspective for analyzing the source and synthetic pathway of HBGA-like molecules in oysters.

Sea ice surface temperature (IST) is an important indicator of environmental changes in the Arctic Ocean. In this study, the relative performance of four mainstream IST records, i.e. airborne IST, infrared radiometer measured IST (IR IST), longwave radiation derived IST (LWR IST), and snow and ice mass balance array buoy derived IST (Buoy IST), were evaluated against the MODIS IST product. Bias, standard deviation (STD), and root mean square error (RMSE) were used to evaluate the data quality. Results revealed that airborne IST had the best accuracy, which was 0.21 K colder than MODIS IST, with STD of 1.46 K and RMSE of 1.47 K. Ground-based ISTs were biased with each other but all warmer than the MODIS IST. The IR IST had the best overall accuracy (bias = 0.55 K; STD = 1.52 K; RMSE = 1.61 K), while the LWR IST was the noisiest measurement with the largest outlier data percent. Besides, co-located IR and LWR ISTs were more consistent than any type of evaluated IST against MODIS IST (correlation coefficient = 0.99). Airborne and IR ISTs are thus the premier choice for monitoring the rapidly changing Arctic sea ice, together with satellite observations.

Seahorses may have required adaptations to avoid immunological rejection of allogenic embryos by males. The authors show that a single substitution in tlx1 is associated with loss of the spleen in seahorses, and that across the clade, degree of male pregnancy is negatively correlated with immune gene repertoire complexity.

The globalization of island studies has resulted in a greater recognition by island studies scholars of the need for more regional-based island research to encompass the diversity of island knowledge and experiences. Island research in East Asia provides examples of both English and native language perspectives across four distinct socio-cultural contexts: Japan, South Korea, the Chinese mainland, and Taiwan. Historical literature and contemporary scholarly research provide a context for understanding: (1) how East Asian researchers engaged with island studies and islandness; and (2) how East Asian socio-economic development policies reflect understandings of islandness locally and across the region. We found similarities among the four East Asian regions, but also variations based on different domestic political perspectives. Given the late start of island research in East Asia, many topics await more detailed future examination.

Ocean antibiotics pose substantial risks to the adaptation and lifespan of marine organisms. Seahorses are unique owing to the occurrence of brood pouches, male pregnancy, and loss of gut-associated lymphatic tissues and spleen, which lead to increased sensitivity to environmental changes. This study evaluated the changes in microbial diversity and immune responses within the gut and brood pouch in the lined seahorse Hippocampus erectus under chronic exposure to environmental levels of triclosan (TCS) and sulfamethoxazole (SMX), which are common antibiotics in coastal regions. The results showed that microbial abundance and diversity within the gut and brood pouch of seahorses were significantly changed following antibiotics treatment, with the expression of core genes involved in immunity, metabolism, and circadian rhythm processes evidently regulated. Notably, the abundance of potential pathogens in brood pouches was considerably increased upon treatment with SMX. Transcriptome analysis revealed that the expression of toll-like receptors, c-type lectins, and inflammatory cytokine genes in brood pouches was significantly upregulated. Notably, some essential genes related to male pregnancy significantly varied after antibiotic treatment, implying potential effects on seahorse reproduction. This study provides insights into the physiological adaptation of marine animals to environmental changes resulting from human activity.

The circadian clock governs activity of many physiological processes, thereby playing a pivotal role in human health. Circadian disruption is closely associated with cancer development; in particular, recent discoveries have provided strong evidence supporting specific functions of different molecular clock components in either promoting or inhibiting tumorigenesis. This narrative review aims to summarize the existing data on molecular connections between the clock and cancer. These results along with future efforts pave the road to targeting the circadian clock as a novel pathway for therapeutic intervention. Given the implications of chrono-nutrition interventions such as time-restricted feeding in extending lifespan, chrono-nutrition may have preventive and therapeutic applications for individuals with and at-risk of age-related diseases including cancer.

Objectives: Vibrio parahaemolyticus is an international foodborne pathogen that has caused many human infection cases. The emergence of antimicrobial resistance (AMR) in V. parahaemolyticus strains can hinder the curative effect of antimicrobials. The aim of this study was therefore to investigate the whole genome sequence of V. parahaemolyticus Vp2015094 from seafood and to explore the genetic mechanisms of AMR. Methods: The whole genome sequencing was performed using PacBio combined with Illumina platform. The antimicrobial resistance genes (ARGs) were detected by Resistance Gene Identifier in the Comprehensive Antibiotic Resistance Database. The antimicrobial-resistant plasmid of Vp2015094 was compared with other similar plasmids from different sources. Results: Vibrio parahaemolyticus Vp2015094 contained two chromosomes and two plasmids. ARGs were identified on chromosome and plasmid pVp94-1. Plasmid pVp94-1 carried tetracycline resistance genes (tetB, tetM, tetR, tetC), aminoglycoside resistance genes (aph(3′')-Ib, aph(6)-Id), sulphonamide resistance genes (sul2), diaminopyrimidine resistance gene (dfrA6), fluoroquinolone resistance gene (qnrVC6), phenicol resistance gene (floR) and penam resistance gene (blaCARB-19), which were surrounded by transposase genes. Plasmid pVp94-1 was a conjugative plasmid with a high transfer frequency. Conclusion: Vibrio parahaemolyticus Vp2015094 exhibited multi-antimicrobial resistance that was mediated by chromosome and plasmid. Plasmid and transposon were speculated to be responsible for the dissemination of ARGs. This study provided an understanding of the genetic mechanisms of AMR of V. parahaemolyticus from seafood, which needs continued monitoring.

The characteristics of sea ice leads (SILs) in the Weddell Sea are an important basis for understanding the mechanism of the atmosphere–ocean system in the Southern Ocean. In this study, we derived the sea ice surface temperature (IST) of the Weddell Sea from MODIS thermal images and then generated a daily SIL map for 2015 and 2022 by utilizing the iterative threshold method on the optimised MOD35 cloud-masked IST. The results showed that SIL variations in the Weddell Sea presented remarkable seasonal characteristics. The trend of the SIL area exhibited an initial rise followed by a decline from January to December, characterised by lower values in spring and summer and higher values in fall and winter. SILs in the Weddell Sea were predominantly concentrated between 70~78°S and 60~30°W. The coastal spatial distribution density of the SILs exceeded that of offshore regions, peaking near the Antarctic Peninsula and then near Queen Maud Land. The SIL variation was mainly influenced by dynamical factors, and there were strong positive correlations between the wind field, ocean currents, and sea-ice motion.

Coal tar pitch (CTP) is a high-quality raw material for producing functional carbon materials owing to its high carbon yield and high degree of condensation. The rational structure regulation of CTP-derived carbon materials is paramount for their special application. Herein, a green template strategy is proposed to fabricate hierarchically porous carbon (HPC) and employ it as the anode material for lithium-ion batteries. It can be demonstrated that the mass ratio of the template (KHCO3) and carbon source (CTP) significantly influences the microstructure and electrochemical performances of HPC. HPC-3 synthesized by a mass ratio of 3:1 shows a coral-like lamellar nanostructure with high specific surface area, developed nanopores, and ample defects, enabling fast and high-flux lithium storage. Thus, the HPC-3 electrode achieves an excellent rate capacity of 219 mAh g−1 at 10 A g−1 and maintains a high discharge capacity of 660 mAh g−1 after 1400 cycles at 1 A g−1. This work takes a step towards the high-value-added and green utilization of CTP and offers a promising solution for the sustainable production of advanced carbon electrode materials.

The spatial structure of Bed and Breakfast (B&B) development plays a crucial role in promoting integrated urban–rural development. However, existing B&B research has predominantly focused on single large cities, neglecting to explore the spatial patterns of B&B development and their influencing factors from the perspective of urban–rural differences. To address this gap, we conducted a comprehensive case study in an all-for-one tourism demonstration area in Hainan Province, China. We adopt geospatial analysis methods and ridge regression models to investigate the characteristics of urban–rural disparities in B&B distribution and to identify the primary factors influencing their spatial arrangement. The research findings reveal valuable insights: (1) B&B establishments in the tourism demonstration area exhibit clustering with notable variations in clustering intensity between urban and rural regions; (2) essential factors affecting the spatial distribution of B&Bs include transportation accessibility, reliance on tourism attractions, B&B development infrastructure, and the availability of living services; (3) tourism resource dependence emerges as the most significant driving force behind B&B agglomerations in the tourism demonstration area; and (4) road network density, hotel service availability, and neighborhood residential density are three additional critical factors affecting B&B distribution, with their influence varying between urban and rural B&Bs. Based on these key findings, we propose development strategies for optimizing B&Bs’ spatial structure in the tourism demonstration area and outline a blueprint for fostering integrated urban–rural development.

Genome-wide occupancy of the master circadian clock transcription factor BMAL1::CLOCK varies across tissues and is reprogrammed in cancers, but how specificity is governed is not known. Here the authors show BMAL1::CLOCK in liver tissue is guided by chromatin accessibility remodeled by HNF4A, shedding new lights onto mechanisms of dysregulated circadian rhythms in hepatocarcinoma.

Diffusing fluid from methane seepage in cold seep field creates zones with physicochemical gradients and divergent ecosystems like the mussel beds and clam beds. Three species of brittle stars (Ophiuroidea) were discovered in the Haima cold seep fields, of which Ophiophthalmus serratus and Histampica haimaensis were found on top of or within mussel beds and clam beds, whereas Amphiura sp. was only collected from muds in the clam bed assemblage. Here, we evaluated the genetic signatures of micro-environmental adaptation of brittle stars to cold seep through the comparison of mitogenomes. This study provided two complete mitogenome sequences of O. serratus and Amphiura sp. and compared with those of H. haimaensis and other non-seep species. We found that the split events of the seep and non-seep species were as ancient as the Cretaceous period (∼148–98 Mya). O. serratus and H. haimaensis display rapid residue mutation and mitogenome rearrangements compared to their shallow or deep-sea relatives, in contrast, Amphiura sp. only show medium, regardless of nucleotide mutation rate or mitogenome rearrangement, which may correlate with their adaptation to one or two micro-ecosystems. Furthermore, we identified 10 positively selected residues in ND4 in the Amphiura sp. lineage, suggesting important roles of the dehydrogenase complex in Amphiura sp. adaptive to the cold seep environment. Our results shed light on the different evolutionary strategies during colonization in different micro-environments.

From the emotional perspective, this study explores how team leader’s conflict management styles affect team passion. A theoretical model is constructed which describes the mediating role of positive team emotional climate and the moderating impact of team emotional intelligence. We collect 101 teams paired data including 101 team leaders and 383 team members to test theoretical model. It is shown that leader’s cooperative conflict management style has a significant positive effect on both positive team emotional climate and team passion. Meanwhile, positive team emotional climate plays a mediating role between leader’s cooperative conflict management style and team passion. In addition, team emotional intelligence has a moderating effect between leader’s cooperative style and positive team emotional climate. This study not only provides a new perspective for follow-up research but also expands the research scope of impacts of conflict management styles. In addition, this study forms the underlying mechanism of team leader’s conflict management styles on team passion from the emotional perspective and investigates the mediating effect and moderating effect of emotional variable, which broadens the research on the mechanisms of conflict management styles on team outcomes to a certain extent.

A new seahorse genome and the re-sequenced genomes of 21 other species shed new light on the evolutionary origin and global dispersal routes of seahorses, and show that bony spines—a key adaptation against predation—probably evolved multiple times via independent substitutions in the bmp3 gene.

Viviparity has originated independently more than 150 times in vertebrates, while the male pregnancy only emerged in Syngnathidae fishes, such as seahorses. The typical male pregnancy seahorses have closed sophisticated brood pouch that act as both uterus and placenta, representing an excellent model system for studying the evolutionary process of paternal immune protection. Phylogenetic analysis indicated that the hampII gene family has multiple tandem duplicated genes and shows independent lineage-specific expansion in seahorses, and they had the highest ratio of nonsynonymous substitutions to synonymous substitutions (dN/dS) in the seahorse phylogenetic branch. The expression levels of hampIIs in the brood pouch placenta were significantly higher during pregnancy than non-pregnancy. Both LPS stimulation test in vivo and cytotoxicity test in vitro proved the immunological protection function of hampIIs against pathogen infection in seahorse. Besides, seahorse hampII peptides exhibit weaker antibacterial function, but stronger agglutination and free endotoxin inhibition. We assumed that the modified immunological function seemed to be a trade-off between the resistance to microbial attack and offspring protection. In brief, this study suggests that the rapid co-option of hampIIs contributes to the evolutionary adaption to paternal immune care during male pregnancy.

Objective To analyze the virulence genes and antimicrobial resistance of 140 Vibrio parahaemolyticus strains from shellfish collected from farms and markets. Methods Virulence genes and antimicrobial resistance genes were detected by PCR amplification method, and antimicrobial resistance was determined by disk diffusion test. Results None of the 140 strains carried tdh gene, and trh gene was detected in one strain. All strains were resistant to ampicillin, some strains were resistant to cefazolin, cefuroxime sodium, streptomycin, amikacin, sulphamethoxazole/trimethoprim, tetracycline and florfenicol. All strains were sensitive to cefepime, gentamicin, imipenem, meropenem, ofloxacin and doxycycline. Six strains showed multi-antimicrobial resistance to two or more categories of antimicrobials. The resistance genes strA and strB were detected in streptomycin resistant strains, tetA was detected in tetracycline resistant strains, sul2 was detected in sulphamethoxazole/trimethoprim resistant strains, and floR was detected in florfenicol resistant strain. Conclusion Most of Vibrio parahaemolyticus strains didn't carry virulence genes and showed antimicrobial resistance to a certain extent. Some strains had more than one antimicrobial resistance gene, which exhibited multi-antimicrobial resistance. These results indicated that enhancing the monitoring of pathogenicity and antimicrobial resistance of Vibrio parahaemolyticus in aquatic products should be continued.

A new species of grouper, Epinephelus tankahkeei sp. nov. is described from the South China Sea based on examination of morphological and molecular characteristics. This new species has been treated as, and is similar to, its congener E. chlorostigma. Epinephelus tankahkeei sp. nov. can be distinguished from E. chlorostigma by the following combination of characters: a convex anal fin; closer dark spots on the body; a lack of dark spots on the abdomen, cheek, and pectoral fin; the absence of a clear posterior white margin on the caudal fin. Molecular analyses of the mitochondrial COI sequence variation, genetic distances, and a phylogeny, all highly support E. tankahkeei sp. nov. as a distinct species. A key to E. tankahkeei sp. nov. and its most closely related species is provided.

Abstract Background and aims Colorectal cancer (CRC) is a major killer. Host immunity is important in tumorigenesis. Direct comparison among IL-36α, IL-36β and IL-36γ in the prognosis of CRC is unclear. Methods CRC tissue arrays were generated from colorectostomy samples with TNM stage, invasion depth and the demography of these patients (n = 185). Using immunohistochemistry/histopathology, IL-36α, IL-36β and IL-36γ were determined, in comparison to non-cancer tissues. Results A significant association was observed between colonic IL-36α, IL-36β or IL-36γ and the presence of cancer (with all P

Few fishes have evolved elevated body temperatures compared with ambient temperatures, and only in opah (Lampris spp) is the entire body affected. To understand the molecular basis of endothermy, we analyzed the opah genome and identified 23 genes with convergent amino acid substitutions across fish, birds, and mammals, including slc8b1, which encodes the mitochondrial Na+/Ca2+ exchanger and is essential for heart function and metabolic heat production. Among endothermic fishes, 44 convergent genes with suggestive metabolic functions were identified, such as glrx3, encoding a crucial protein for hemoglobin maturation. Numerous genes involved in the production and retention of metabolic heat were also found to be under positive selection. Analyses of opah's unique inner-heat-producing pectoral muscle layer (PMI), an evolutionary key innovation, revealed that many proteins were co-opted from dorsal swimming muscles for thermogenesis and oxidative phosphorylation. Thus, the opah genome provides valuable resources and opportunities to uncover the genetic basis of thermal adaptations in fish. Public summary: • Endothermy has evolved multiple times in fishes (teleosts and chondrichthyans) • Opah genome explaining genetic changes in heat production and the sensory and immune system • Convergent evolution of genes in endothermic vertebrate lineages was investigated • Analyses of the pectoral muscle of opah revealed numerous highly expressed genes for thermogenesis

Many small island destinations owe their spatial character to their entanglements with stakeholders involved in the arts. Space is the dynamic outcome of complex relational processes, which makes it impossible to identify a straightforward development path — including when it comes to the arts and tourism. Using assemblage thinking, we scrutinize the different translocal processes influencing art-based tourism activities on Bornholm, Denmark and Naoshima, Japan. On these islands, artists, investors, residents, destination managers, creative individuals, and government officials are all involved in networks and negotiations that form complex translocal assemblages of art and tourism. The craft-artists of Bornholm took advantage of regional development policies aimed at fostering rural tourism development, and subsequently established a destination known for quality professional craftart. On Naoshima, top-down corporate investments in large-scale art developments have clashed with local stakes in rural revitalization. These top-down projects have attracted creative in-migrants who have further turned Naoshima into a hybrid space. While Bornholm’s entanglement with the arts stems from the possibilities generated by its spatial evolution, Naoshima’s involvement with the arts first led to reterritorialization and then creative enhancement. Both islands are, thus, distinct loci of translocal art trajectories.

Background:BRCA1/2 mutations are closely related to high lifetime risk of breast cancer (BC). The objective of this study was to identify the genes, regulators, and immune-associated patterns underlying disease pathology in BC with BRCA1/2 somatic mutations and their associations with clinical traits.Methods: RNA sequencing data and clinical information from The Cancer Genome Atlas (TCGA; N = 36 BRCA1-mutant BC; N = 49 BRCA2-mutant BC; and N = 117 BRCA1/2-wild-type BC samples) were used for discovery, which included consensus network analysis, function enrichment, and analysis of hub genes; other TCGA data (N = 117 triple-negative BC) and two Gene Expression Omnibus database expression profiles were used as validation cohorts.Results: Consensus network analysis helped to identify specific co-expressed modules that showed positive correlations with tumor stage, number of positive lymph nodes, and margin status in BRCA1/2-mutant BC but lacking correlations in BRCA1/2-wild-type BC. Functional enrichment suggested potential mechanisms in BRCA1/2 carriers that could regulate the cell cycle, immune response, cellular metabolic processes, and cell migration, via enriched pathways including p53 and JAK–STAT signaling. Consensus network analysis identified the specific and common carcinogenic mechanisms involving BRCA mutations. Regulators cross-linking these modules include E2F or IRF transcription factor family, associated with cell cycle or immune response regulation module, respectively. Eight hub genes, including ISG15, BUB1, and TTK, were upregulated in several BRCA1/2-mutant BC datasets and showed prognostic value in BC. Furthermore, their genetic expression was related to higher levels of immune infiltration in BRCA1/2-mutant BC, which manifested as recruitment of T helper cells (Th1 cells), follicular helper T cells, and regulatory T cells, and T cell exhaustion. Moreover, important indicators for evaluation of BC immunotherapy, tumor mutational burden and neoantigen load also positively correlated with expression of some hub genes.Conclusion: We constructed a BRCA1/2 mutation-type-specific co-expressed gene network with related transcription factors and immune-associated patterns that could regulate and influence tumor metastasis and immune microenvironment, providing novel insights into the pathological process of this disease and the corresponding BRCA mutations.

This paper introduces a Patron Counting and Analysis (PCA) system that leverages Wi-Fi-connection data to monitor space utilization and analyze visitor patterns in academic libraries. The PCA system offers real-time crowding information to the public and a comprehensive visitor analysis dashboard for library administrators. The system's development was driven by the need for occupancy restrictions during the pandemic, ensuring a spacious environment for library visitors as well as balancing between efficient utilization and adhering to social distancing regulations. Traditional methods of patron behavior performance and library spatial analysis, such as manual head counting or card-swiping systems, often incur additional costs for labor, hardware installation, or software subscription. The PCA system, however, utilizes existing Wi-Fi-connection data, providing a cost-effective solution to represent patron demographics and spatial usage. Limitations may arise when patrons do not carry Wi-Fi-enabled devices or during periods of low Wi-Fi service functionality. Implemented in Node.js and integrated with Python Flask framework and related libraries, the PCA system was piloted at the King Library in Miami University, successfully demonstrating a high validity compared to manually collected data. It filters out noise and redundancy, visualizes the occupancy index meter in real time, and generates statistical reports by linking user IDs with demographic information. The PCA system's reliability was validated through manually head counting data collected at the King Library in Miami University, establishing it as a reliable tool for library space management and patron analysis.

Clownfish can be an excellent research model for investigating the socially-controlled sexual development of sequential hermaphrodite teleosts. However, the molecular cascades underlying the social cues that orchestrate the sexual development process remain poorly understood. Here, we performed a comparative transcriptomic analysis of gonads from females, males, and nonbreeders of Amphiprion clarkii, which constitute a complete social group, allowing us to investigate the molecular regulatory network under social control. Our analysis highlighted that the gonads of nonbreeders and males exhibited high similarities but were far from females, both in global transcriptomic profiles and histological characteristics, and identified numerous candidate genes involved in sexual development, some well-known and some novel. Significant upregulation of cyp19a1a, foxl2, nr5a1a, wnt4a, hsd3b7, and pgr in females provides strong evidence for the importance of steroidogenesis in ovarian development and maintenance, with cyp19a1a playing a central role. Amh and sox8 are two potential key factors that may regulate testicular tissue development in early and late stages, respectively, as they are expressed at higher levels in males than in females, but with slightly different expression timings. Unlike previous descriptions in other fishes, the unique expression pattern of dmrt1 in A. clarkii implied its potential function in both male and female gonads, and we speculated that it might play promoting roles in the early development of both testicular and ovarian tissues.

The low-level dilemma of farmers’ Socialized Agricultural Services (SAS) purchases is a realistic problem facing the construction of socialized service systems in China’s major grain-producing areas. Existing research lacks the necessary attention on the decision-making mechanism of farmers’ purchases of SAS, resulting in a large gap between theoretical research and real-world problems. Based on the data of 638 households in China’s main grain-producing areas, this paper empirically analyzes the influence mechanism of heterogeneous land scale on deviation between farmers’ willingness and behavior to purchase SAS, and discusses the heterogeneity of the influence mechanism under different production links. The findings indicate that the degree of divergence between farmers’ willingness to purchase SAS and their behavior is large. (1) There is a significant threshold effect of land scale on the degree of divergence between farmers’ willingness and behavior to purchase SAS, and the threshold value is ten mu. The increase in land scale will significantly enlarge the divergence between small-scale farmers’ purchase willingness and behavior. However, it will significantly reduce the divergence between large-scale farmers’ purchase willingness and behavior. (2) From the demand side, household income and health will significantly reduce the divergence among farmers of different business sizes. Family burden and land fragmentation are essential factors affecting the degree of divergence of small-scale farmers, while education and the value of their own machinery significantly affect the degree of divergence of large-scale farmers. From the supply side, whether there are local suppliers and their matching degree with farmers significantly reduces the divergence among farmers of different business scales. However, small-scale farmers are more sensitive to the price and quality of agricultural services, while large-scale farmers have higher requirements for the degree of regulation of supplying agents. (3) There is heterogeneity in the influence mechanisms of divergence between farmers’ willingness to purchase SAS and their behavior under different product segments. In addition, our findings highlight how to realize the transformation from declarative preference to explicit preference in promoting farmers’ purchases of SAS.

Perfluorooctanoic acid (PFOA), a persistent environmental contaminant, resists environmental degradation and bioaccumulates in food chains. Lots of literatures have proved that PFOA exposure could disrupt detoxifying function in a variety of organisms, however, it still remained poorly known about this in mollusk. Here, we examined physiological, transcriptomic, and metabolomic responses to PFOA in Mytilus edulis, a model organism frequently used in studies of aquatic pollution. We aimed to characterize PFOA-induced stress responses and detoxification mechanisms. PFOA exposure significantly altered antioxidant enzyme activity levels and the abundances of lipid peroxidation products. In addition, transcriptomic analysis indicated that several genes associated with oxidative stress and detoxication were differentially expressed after PFOA exposure. In combination, transcriptomic and metabolomic analyses showed that PFOA exposure disturbed several metabolic processes in M. edulis, including the lipid metabolism, amino acid metabolism, and carbohydrate metabolism etc. Molecular examination and enzymes assay of PFOA-exposed M. edulis after a 7-day depuration period still did not recover to control levels. The Pathway enrichment analysis proved that several pathways related to detoxification, such as c-Jun N-terminal kinase (JNK) and p38-dependent mitogen-activated protein kinase (MAPK) pathway, Peroxisome proliferator-activated receptor γ (PPARγ) pathway etc, were obviously affected. The present work verifies firstly PFOA disruption to molluscan detoxification and identifies the key pathways to understand the molecular mechanisms thereof. This study provides new insights into the detoxication disruption invoked in response to PFOA exposure in M. edulis.

Summary: Seahorses epitomize the exuberance of evolution. They have the unique characteristic of male pregnancy, which includes the carrying of many embryos in a brood pouch that incubates and nourishes the embryos, similar to the mammalian placenta. However, the regulatory networks underlying brood pouch formation and pregnancy remain largely unknown. In this study, comparative transcriptomic and metabolomic profiling on the lined seahorse Hippocampus erectus, with unformed, newly formed, and pregnant brood pouches identified a total of 141 and 2,533 differentially expressed genes together with 73 and 121 significantly differential metabolites related to brood pouch formation and pregnancy, respectively. Specifically, integrative omics analysis revealed that retinoic acid (RA) synthesis and signaling pathway played essential roles in the formation of the brood pouch and pregnancy. RA might function upstream of testosterone and progesterone, thereby directly influencing brood pouch formation by regulating the expression of fshr and cyp7a1. Our results also revealed that RA regulates antioxidant defenses, particularly during male pregnancy. Alternatively, pregnancy caused a consistent decrease in RA, canthaxanthin, astaxanthin, and glutathione synthetase, and an increase in susceptibility to oxidative stress, which may balance brood pouch development and reproduction in seahorses and pave the way to successful gestation.

Human BCA2/RNF115/Rabring7 (hBCA2) is a RING type E3 ubiquitin ligase with the ability of autoubiquitination or promoting protein ubiquitination. It also acts as a host restriction factor has BST2-dependent and BST2-independent antiviral activity to inhibit the release of HIV-1. In a previous study, we demonstrated that feline BCA2 (fBCA2) also has E3 ubiquitin ligase activity, although its antiviral mechanism remained unclear. In this study, we showed that fBCA2 can interact with feline BST2 (fBST2) and exhibits an fBST2-independent antiviral function, and the RING domain is necessary for the antiviral activity of fBCA2. fBCA2 could degrade HIV-1 Gag and restrict HIV-1 transcription to counteract HIV-1 but not promote the degradation of HIV-1 through lysosomal. Furthermore, for both fBCA2 and hBCA2, restricting viral transcription is the main anti-FIV mechanism compared to degradation of FIV Gag or promoting viral degradation. Consequently, transcriptional regulation of HIV or FIV by BCA2 should be the primary restriction mechanism, even though the degradation mechanism is different when BCA2 counteracts HIV or FIV. This may be due to BCA2 has a special preference in antiviral mechanism in the transmission of primate or non-primate retroviruses.

In this study, we retrieve an Arctic sea ice lead fraction from AMSR2 passive microwave data in winter from 2012 to 2020 based on an algorithm developed for AMSR-E data. The derived AMSR2 sea ice lead fraction is validated against MODIS images. The results show that the derived AMSR2 sea ice lead detects approximately 50% of the ice leads shown in the MODIS images, which is close to the amount of sea ice lead detected from the AMSR-E data from 2002 to 2011. Utilizing the retrievals from both the AMSR-E and AMSR2, our analysis shows no significant trend, but moderate interannual variation exists for the ice lead fraction in the Arctic basin scale over the past two decades. The maximum width and total length of sea ice lead show a significant decreasing trend for the whole Arctic, but the mean width does not exhibit a significant change over the studied period. In the Beaufort Sea the lead fraction varies from 2.06% to 12.35%, with a mean value of 5.72%. In the Greenland Sea the mean lead fraction over the studied period is 5.77%, and there is a significant increase in the lead fraction, with a rate of 0.13% per year. The maximum width in the Greenland Sea is substantially higher than that of other regions, and the mean width increases significantly.

Dome A is the summit of the Antarctic plateau, where the Chinese Kunlun inland station is located. Due to its unique location and high altitude, Dome A provides an important observatory site in analyzing global climate change. However, before the arrival of the Chinese Antarctic expedition in 2005, near-surface air temperatures had not been recorded in the region. In this study, we used meteorological parameters, such as ice surface temperature, radiation, wind speed, and cloud type, to build a reliable model for air temperature estimation. Three models (linear regression, random forest, and deep neural network) were developed based on various input datasets: seasonal factors, skin temperature, shortwave radiation, cloud type, longwave radiation from AVHRR-X products, and wind speed from MERRA-2 reanalysis data. In situ air temperatures from 2010 to 2015 were used for training, while 2005–2009 and 2016–2020 measurements were used for model validation. The results showed that random forest and deep neural network outperformed the linear regression model. In both methods, the 2005–2009 estimates (average bias = 0.86 °C and 1 °C) were more accurate than the 2016–2020 values (average bias = 1.04 °C and 1.26 °C). We conclude that the air temperature at Dome A can be accurately estimated (with an average bias less than 1.3 °C and RMSE around 3 °C) from meteorological parameters using random forest or a deep neural network.

Abstract In this paper, we consider the following nonlinear Schrödinger system involving the fractional Laplacian operator: {(−Δ)α2u+au=f(v),(−Δ)β2v+bv=g(u),on Ω⫅Rn, $$ \textstyle\begin{cases} (-\Delta)^{\frac{\alpha}{2}}u+au=f(v), \\ (-\Delta)^{\frac{\beta}{2}}v+bv=g(u), \end{cases}\displaystyle \quad \text{on } \Omega\subseteqq \mathbb{R}^{n}, $$ where a,b≥0 $a,b\geq0$. When Ω is the unit ball or Rn $\mathbb{R}^{n}$, we prove that the solutions (u,v) $(u,v)$ are radially symmetric and decreasing. When Ω is the parabolic domain on Rn $\mathbb{R}^{n}$, we prove that the solutions (u,v) $(u,v)$ are increasing. Furthermore, if Ω is the R+n $\mathbb{R}^{n}_{+}$, then we also derive the nonexistence of positive solutions to the system on the half-space. We assume that the nonlinear terms f, g and the solutions u, v satisfy some amenable conditions in different cases.

Abstract By decomposing functions, we establish some boundedness results for some rough singular integrals on the homogeneous Morrey-Herz spaces M K ˙ q , p ( ⋅ ) α ( ⋅ ) , λ ( R n ) $M\dot{K}_{q, p(\cdot)}^{\alpha(\cdot),\lambda}({\Bbb{ R}}^{n})$ , where the two main indices are variable. The corresponding results as regards their commutators are also considered.

Abstract In this note, we prove that the fractional integral operators from Herz spaces with variable exponent K ˙ p ( ⋅ ) , q α $\dot{K}^{\alpha}_{p(\cdot), q}$ to Lipschitz-type spaces are bounded provided p ( ⋅ ) $p(\cdot)$ is locally log-Hölder continuous and log-Hölder continuous at infinity.

Porcine circovirus-like virus P1 is an important pathogen of the current pig industry, the infection mechanism is not entirely clear. Wnt signaling pathway plays an important role in the growth of young animals and infection of some viruses. This study was designed to demonstrate the effects of P1 infection on the Wnt signaling pathway. In vivo experiments, we demonstrated the down-regulatory effects of P1 infection in piglets and mice on the downstream components expression levels of Wnt signaling pathway, and the effects of Wnt signaling pathway activation on the pathogenesis of P1. In vitro studies, we found P1 infection down-regulated protein level of β-catenin and mRNA level of mmp2, prevented the β-catenin from entering into nucleus, abolished the TCF/LEF promoter activity, proved that P1 could inhibit the activation of Wnt signaling pathway in vitro. Finally, we found that VP1 of P1 virus also had the inhibitory effects on Wnt signaling pathway in vitro, elucidated the mechanism of P1’s inhibitory effects on the Wnt signaling pathway and offered the possibility that the suppression of Wnt signaling pathway was involved in the post-weaning multisystemic wasting syndrome (PMWS), laying a foundation for elucidating the pathogenesis of P1.

In recent years, much attention has been paid to the behavior of passive microwave sea ice concentration (SIC) products for marginal ice zones. Based on the definition of ice edges from ship observations, we identified pseudo-ship observations (PSO) and generated PSO ice edges from twelve cloud-free moderate-resolution imaging spectroradiometer (MODIS) images. Two SIC products of the advanced microwave scanning radiometer 2 (AMSR2) were compared at the PSO ice edges: ARTIST (arctic radiation and turbulence interaction study) sea ice (ASI-SIC) and bootstrap (BST-SIC). The mean values of ASI-SIC pixels located at ice edges were 10.5% and 10.3% for the Arctic and the Antarctic, respectively, and are below the commonly applied 15% threshold, whereas the mean values of corresponding BST-SIC pixels were 23.6% and 27.3%, respectively. The mean values of both ASI-SIC and BST-SIC were lower in summer than in winter. The spatial gaps among the 15% ASI-SIC ice edge, the 15% BST-SIC ice edge and the PSO ice edge were mostly within 35 km, whereas the 15% ASI-SIC ice edge matched better with the PSO ice edge. Results also show that the ice edges were located in the thin ice region, with a mean ice thickness of around 5–8 cm. We conclude that the 15% threshold well determines the ice edge from passive microwave SIC in both the Arctic and the Antarctic.

BST2 (CD317, tetherin, HM1.24) is an interferon-inducible transmembrane protein which can directly inhibit the release of enveloped virus particles from infected cells, and its anti-viral activity is reported to be related to the specific topological arrangement of its four structural domains. The N-terminal cytoplasmic tail of feline BST2 (fBST2) is characterized by a shorter N-terminal region compared to those of other known homologs. In this study, we investigated the functional impact of modifying the cytoplasmic tail region of fBST2 and its molecular mechanism. The fBST2 protein with the addition of a peptide at the N-terminus retained anti-release activity against human immunodeficiency virus type-1 and pseudovirus based on feline immunodeficiency virus at a weaker level compared with the wild-type fBST2. However, the fBST2 protein with addition of a peptide internally in the ectodomain proximal to the GPI anchor still retained its anti-viral activity well. Notably, the N-glycosylation state and the cell surface level of the N-terminally modified variants were unlike those of the wild-type protein, while no difference was observed in their intracellular localizations. However, in contrast to human BST2, the wild-type fBST2 did not show the ability to activate NF-κB. Consistent with previous reports, our findings showed that adding a peptide in the cytoplasmic tail region of fBST2 may influence its anti-viral activity. The shorter N-terminal cytoplasmic region of fBST2 compared with human BST2 did not apparently affect its anti-viral activity, which is independent of its N-glycosylation and ability to activate NF-κB.

Groupers of the family Epinephelidae are a diverse and economically valuable group of reef fishes. To investigate the evolution of their mitochondrial genomes we characterized and compared these genomes among 22 species, 17 newly sequenced. Among these fishes we identified three distinct genome organizations, two of them never previously reported in vertebrates. In 19 of these species, mitochondrial genomes followed the typical vertebrate canonical organization with 13 protein-coding genes, 22 tRNAs, two rRNAs, and a non-coding control region. Differing from this, members of genus Variola have an extra tRNA-Ile between tRNA-Val and 16S rRNA. Evidence suggests that this evolved from tRNA-Val via a duplication event due to slipped strand mispairing during replication. Additionally, Cephalopholisargus has an extra tRNA-Asp in the midst of the control region, likely resulting from long-range duplication of the canonical tRNA-Asp through illicit priming of mitochondrial replication by tRNAs. Along with their gene contents, we characterized the regulatory elements of these mitochondrial genomes' control regions, including putative termination-associated sequences and conserved sequence blocks. Looking at the mitochondrial genomic constituents, rRNA and tRNA are the most conserved, followed by protein-coding genes, and non-coding regions are the most divergent. Divergence rates vary among the protein-coding genes, and the three cytochrome oxidase subunits (COI, II, III) are the most conserved, while NADH dehydrogenase subunit 6 (ND6) and the ATP synthase subunit 8 (ATP8) are the most divergent. We then tested the phylogenetic utility of this new mt genome data using 12 protein-coding genes of 48 species from the suborder Percoidei. From this, we provide further support for the elevation of the subfamily Epinephelinae to family Epinephelidae, the resurrection of the genus Hyporthodus, and the combination of the monotypic genera Anyperodon and Cromileptes to genus Epinephelus, and Aethaloperca to genus Cephalopholis.

By decomposing functions, we establish estimates for higher order commutators generated by fractional integral with BMO functions or the Lipschitz functions on the homogeneous Herz spaces with variable exponent. These estimates extend some known results in the literatures.

In response to DNA damage, the eukaryotic genome surveillance system activates a checkpoint kinase cascade. In the fission yeast Schizosaccharomyces pombe, checkpoint protein Crb2 is essential for DNA damage-induced activation of downstream effector kinase Chk1. The mechanism by which Crb2 mediates Chk1 activation is unknown. Here, we show that Crb2 recruits Chk1 to double-strand breaks (DSBs) through a direct physical interaction. A pair of conserved SQ/TQ motifs in Crb2, which are consensus phosphorylation sites of upstream kinase Rad3, is required for Chk1 recruitment and activation. Mutating both of these motifs renders Crb2 defective in activating Chk1. Tethering Crb2 and Chk1 together can rescue the SQ/TQ mutations, suggesting that the main function of these phosphorylation sites is promoting interactions between Crb2 and Chk1. A 19-amino-acid peptide containing these SQ/TQ motifs is sufficient for Chk1 binding in vitro when one of the motifs is phosphorylated. Remarkably, the same peptide, when tethered to DSBs by fusing with either recombination protein Rad22/Rad52 or multi-functional scaffolding protein Rad4/Cut5, can rescue the checkpoint defect of crb2Δ. The Rad22 fusion can even bypass the need for Rad9-Rad1-Hus1 (9-1-1) complex in checkpoint activation. These results suggest that the main role of Crb2 and 9-1-1 in DNA damage checkpoint signaling is recruiting Chk1 to sites of DNA lesions.

BACKGROUND: Cephalopoda are a class of Mollusca species found in all the world's oceans. They are an important model organism in neurobiology. Unfortunately, the lack of neuronal molecular sequences, such as ESTs, transcriptomic or genomic information, has limited the development of molecular neurobiology research in this unique model organism. RESULTS: With high-throughput Illumina Solexa sequencing technology, we have generated 59,859 high quality sequences from 12,918,391 paired-end reads. Using BLASTx/BLASTn, 12,227 contigs have blast hits in the Swissprot, NR protein database and NT nucleotide database with E-value cutoff 1e(-5). The comparison between the Octopus vulgaris central nervous system (CNS) library and the Aplysia californica/Lymnaea stagnalis CNS ESTs library yielded 5.93%/13.45% of O. vulgaris sequences with significant matches (1e(-5)) using BLASTn/tBLASTx. Meanwhile the hit percentage of the recently published Schistocerca gregaria, Tilapia or Hirudo medicinalis CNS library to the O. vulgaris CNS library is 21.03%-46.19%. We constructed the Phylogenetic tree using two genes related to CNS function, Synaptotagmin-7 and Synaptophysin. Lastly, we demonstrated that O. vulgaris may have a vertebrate-like Blood-Brain Barrier based on bioinformatic analysis. CONCLUSION: This study provides a mass of molecular information that will contribute to further molecular biology research on O. vulgaris. In our presentation of the first CNS transcriptome analysis of O. vulgaris, we hope to accelerate the study of functional molecular neurobiology and comparative evolutionary biology.