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141 results on '"Meng, Xiao-Lin"'

38. Biological characteristics of recombinant enhancin ofTrichoplusia nigranulovirus expressed inEscherichia coli

Author

Yuan Zhe-ming, Liu ShuSheng, and Meng Xiao-lin

Subjects
Gel electrophoresis, biology, Sodium, fungi, Nuclear Polyhedrosis Virus, chemistry.chemical_element, Ethylenediaminetetraacetic acid, Helicoverpa armigera, medicine.disease_cause, biology.organism_classification, Inclusion bodies, law.invention, chemistry.chemical_compound, Biochemistry, chemistry, law, medicine, Recombinant DNA, Agronomy and Crop Science, Escherichia coli, Biotechnology
Abstract

A recombinant enhancin fromTrichoplusia nigranulovirus, expressed inEscherichia coliand named P96, had been shown in a previous study to increase significantly the efficacy of bio-insecticides applied to insect pests. Here, several biological characteristics were clarified to provide further proof for the activities of P96. The inclusion bodies formed by P96 were quite stable when expressed inE. coli; however, they could be effectively dissolved in sodium carbonate (Na2CO3) solution or in the midgut digestive secretion of the fifth-instar larvae of the cotton bollworm,Helicoverpa armigera,in vitro. According to sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), the peritrophic membrane of the cotton bollworm was clearly decomposed by P96. With the increase of P96 concentration, the mortality of the cotton bollworm caused by nuclear polyhedrosis virus increased up to its saturation concentration, 6.76×105inclusion bodies/ml. The bioactivity of P96 was strongly inhibited by ethylenediaminetetraacetic acid (EDTA), which formed a complex compound with the metal ion (Zn2+) in the protein.

Published
2006

42. Expression of E1 gene of a hepatitis C virus inE. coli and protein purification

Author

Zhu Ying, Hu Min, Ye Chanying, Min Lei, XU Jin-ping, Gao Jin-rong, Wu Zhenhue, Meng Xiao-lin, and YE Lin-bai

Subjects
Multidisciplinary, Expression vector, biology, Chemistry, lac operon, biology.organism_classification, Molecular biology, law.invention, law, Complementary DNA, Protein purification, Protein A/G, biology.protein, Recombinant DNA, Antibody, Bacteria
Abstract

The cDNA containing full encoding region of E1 antigen of HCV was cloned into an expression plasmid pRSETHisB. The recombinant plasmid pRSETE1 was introduced into the BL21 (DE3) strain ofE. coli. The engineering bacteria harbouring the pRSETE1 was cultivated in 2YT medium at 37°C. When the Expression of E1 protein was induced by 1 mmol IPTG, the bacteria was killed and the number of living cell was droped down from 107 to 103 cell/mL one hour post induction. Suggest that E1 protein is poisoned toE. coli. However, the 26kD polypeptide of E1 fussion protein still synthesized in appropriate condition. The expression level was about 10% of total protein 4 h after inducing. The E1 protin was purified by Ni2+-NTA-Agarose column chromatography to homogeneous. The purified E1 protein was sensitive and specific in reaction with anti-HCV antibody in sera.

Published
1996

45. Gene cloning, expression, and characterization of a novel β-mannanase from the endophyte Paenibacillus sp. CH-3.

Author

Zhang, Jian‐Xin, Chen, Ze‐Tian, Meng, Xiao‐Lin, Mu, Guang‐Ya, Hu, Wen‐Bo, Zhao, Jie, and Nie, Guo‐Xing

Subjects
PAENIBACILLUS, BLACK locust, MOLECULAR cloning, ANAEROBIC bacteria, SEEDS
Abstract

An endophyte with high yield of β-mannanase, designated as Paenibacillus sp. CH-3, was isolated from black locust ( Robinia pseudoacacia) seeds. The β-mannanase gene ( manB) has been cloned, expressed, purified, and characterized. With an open reading frame of 984 bp, the manB gene encodes 327 amino acid residues, which belongs to GH family 5. The recombinant β-mannanase (ManB) has an apparent molecular weight of 50.4 kDa with an optimal activity level at pH 7.0 and 45 °C, and it is stable in the range of pH 4-9. By optimizing, the temperature, isopropyl β- d-thiogalactoside concentration, and induction time were 28 °C, 0.05 mM, and 12 H, respectively. The highest activity reached at 1,054.17 U/mL. In addition, ManB had Km values of 7.30, 8.69, and 27.17 mg/mL for locust bean gum (LBG), guar gum, and konjac glucomannan (KGM), respectively. Its catalytic efficiency ( kcat/ Km) was 176.31 mL/(mg·Sec) for LBG, 116.69 mL/(mg·Sec) for guar gum, and 115.49 mL/(mg·Sec) for KGM. The products of glucomannans, which were hydrolyzed by ManB, mainly contain mannobiose, mannotetrose, and a higher manno-oligosaccharide. The characteristics of ManB revealed that it could be exploited as an effective additive in the animal feed industry. [ABSTRACT FROM AUTHOR]

Published
2017
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46. Symbiotic bacteria associated with puffer fish Gastrophysus spadiceusand evaluation of their antimicrobial activities

Author

Wang, Yi-Huan, Salam, Nimaichand, Liu, Qing, Yang, Zi-Wen, Cao, Li-Xiang, Meng, Xiao-Lin, Nie, Guo-Xing, Ju, Jian-Hua, and Li, Wen-Jun

Abstract

The present study reports the diversity of culturable bacteria associated with the puffer fish Gastrophysus spadiceus. During the study, a total of 31 strains affiliated to the genera Pseudomonas, Janthinobacterium, Rahnella,and Psychrobacterwere isolated from liver, intestines, and flesh of G. spadiceus. These strains exhibited a diverse range of metabolites as indicated by the HPLC and TLC profiles of the chemical extracts of their fermentation products. Some of these crude extracts showed strong antimicrobial activities against pathogenic bacterial strains. In addition, few crude extracts exhibit insecticidal activity against Artemia salina.

Published
2017
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