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Your search keyword '"Meng, Shanyu"' showing total 17 results
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17 results on '"Meng, Shanyu"'

16. Effects of ricin on primary pulmonary alveolar macrophages

Author

Guo, Zhendong, primary, Wang, Zhongyi, additional, Meng, Shanyu, additional, Zhao, Zongzheng, additional, Zhang, Chunmao, additional, Fu, Yingying, additional, Li, Jiaming, additional, Nie, Xin, additional, Zhang, Cheng, additional, Liu, Linna, additional, Lu, Bing, additional, and Qian, Jun, additional

Published
2019
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17. Selective Killing of Shiga Toxin-Producing Escherichia coliwith Antibody-Conjugated Chitosan Nanoparticles in the Gastrointestinal Tract

Author

Ma, Zhengxin, Kang, Minyoung, Meng, Shanyu, Tong, Zhaohui, Yoon, Soon-Do, Jang, Yeongseon, and Jeong, K. Casey

Abstract

Shiga toxin-producing Escherichia coli(STEC) are critical foodborne pathogens, which cause serious human health issues, including hemolytic uremic syndrome. Illnesses caused by STEC lack effective treatments that target the elimination of these bacteria from the gastrointestinal tract without causing an adverse effect. Reducing this pathogen from a reservoir of STEC is an effective strategy, but the challenges remain due to the lack of efficient, selective antimicrobial agents. We developed specific antibody-conjugated chitosan nanoparticles (CNs) to selectively target and treat STEC in the gastrointestinal tract. Given the great broad-spectrum antimicrobial activity of CN, we conjugated antibodies to CN. Antibodies were raised and purified from egg yolks after immunization of hens with seven different O-side-chain antigens isolated from STEC (O26, O45, O103, O111, O121, O145, and O157). We prepared CN-immunoglobulin Y (IgY) conjugates by forming amide bonds at different ratios of CN:IgY (10:1, 10:2, and 10:4). The CN-IgY conjugated at a 10:2 ratio demonstrated significantly enhanced antimicrobial activity against E. coliO157:H7. Conjugates of CN and anti-STEC IgY antibodies killed corresponding STEC serotypes specifically and selectively, while showing no significant impact on nontargeted bacteria, including Salmonella entericaand Lactobacillus plantarum. The enhanced antimicrobial activity of CN-IgY against STEC was also confirmed in synthetic intestinal fluid, as well as an in vivo animal model of Caenorhabditis elegans. These results suggest that the CN-IgY conjugates have strong and specific antimicrobial activity and that they are also great candidates to eliminate pathogens selectively in the gastrointestinal tract without inhibiting beneficial bacteria.

Published
2020
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