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4. Jagged1-Notch1 Signaling Pathway Induces M1 Microglia to Disrupt the Barrier Function of Retinal Microvascular Endothelial Cells.

Author

Wu, Xiyu, Zhu, Haoxian, Liu, Junbin, Ouyang, Shuyi, Lyu, Zheng, Jin, Yeanqi, Chen, Xinyu, and Meng, Qianli

Subjects
CELL permeability, TIGHT junctions, VASCULAR endothelial cells, RNA interference, GENE expression
Abstract

Purpose: Microglia-related inflammation is closely linked to the pathogenesis of retinal diseases. The primary objective of this research was to investigate the impact and mechanism of M1 phenotype microglia on the barrier function of retina microvascular endothelial cells. Methods: Quantitative polymerase chain reactions and western blot techniques were utilized to analysis the mRNA and protein expressions of M1 and M2 markers of human microglial clone 3 cell line (HMC3), as well as the levels of Notch ligands and receptors under the intervention of lipopolysaccharide (LPS) or interleukin (IL)-4. ELISA was utilized to detect the pro-inflammatory and anti-inflammatory cytokines from HMC3 cells. The cellular tight junction and apoptosis of human retinal microvascular endothelial cells (HRMECs) were assessed by western blot and fluorescein isothiocyanate-dextran permeability assay. The inhibitors of Notch1 and RNA interference (RNAi) targeting Jagged1 were used to assess their contribution to the barrier function of vascular endothelial cells. Results: Inducible nitric oxide synthase (iNOS) and IL-1β were considerably elevated in LPS-treated HMC3, while CD206 and Arg-1 markedly elevated under IL-4 stimulation. The conditioned medium derived from LPS-treated HMC3 cells promoted permeability, diminished the expression of zonula occludens-1 and Occludin, and elevated the expression of Cleaved caspase-3 in HRMECs. RNAi targeting Jagged1 or Notch1 inhibitor could block M1 HMC3 polarization and maintain barrier function of HRMECs. Conclusion: Our findings suggest that Jagged1-Notch1 signaling pathway induces M1 microglial cells to disrupt the barrier function of HRMECs, which may lead to retinal diseases. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Prevalence and Pattern of Geographic Atrophy in Asia: The Asian Eye Epidemiology Consortium

Author

Saenmee, Achareeya, Cao, Kai, George, Ronnie, Kazakbaeva, Gyulli M., Khalimov, Timur A., Khanna, Rohit C., Kim, Hyun Woong, Kulothungan, Vaitheeswaran, Nangia, Prabhat, Mao, Feifei, Matsuda, Fumihiko, Meng, Qianli, Namba, Hiroyuki, Pokawattana, Nattapol, Oh, Jaeryung, Park, Sang Jun, Ravindran, Ravilla, Sharma, Tarun, Shin, Jae Pil, Surya, Janani, Takahashi, Ayako, Takebayashi, Toru, Tsujikawa, Akitaka, Vashist, Praveen, Wei, Wen Bin, Yang, Xiaohui, Yu, Seung-Young, Zainullin, Rinat M., Zhao, Pei Quan, Rim, Tyler Hyungtaek, Kawasaki, Ryo, Tham, Yih-Chung, Kang, Se Woong, Ruamviboonsuk, Paisan, Bikbov, Mukharram M., Miyake, Masahiro, Hao, Jie, Fletcher, Astrid, Sasaki, Mariko, Nangia, Vinay, Sabanayagam, Charumathi, Yu, Marco, Fujiwara, Kohta, Thapa, Raba, Wong, Ian Y., Kayama, Takamasa, Chen, Shih-Jen, Kuang, Tung-Mei, Yamashita, Hidetoshi, Sundaresan, Periasamy, Chan, Jonathan C., van Rens, G.H.M.B., Sonoda, Koh-Hei, Wang, Ya Xing, Panda-Jonas, Songhomitra, Harada, Sei, Kim, Ramasamy, Ganesan, Suganeswari, Raman, Rajiv, Yamashiro, Kenji, Gilmanshin, Timur R., Jenchitr, Watanee, Park, Kyu Hyung, Gemmy Cheung, Chui Ming, Wong, Tien Yin, Wang, Ningli, Jonas, Jost B., Chakravarthy, Usha, Cheng, Ching-Yu, and Yanagi, Yasuo

Published
2020
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8. Inhibition of Microglia from Releasing Proinflammatory Cytokines Via Translocator Protein Upregulation is Responsible for Neuroprotection of Sodium-Glucose Cotransporter 2 Inhibitor in Diabetic Retinopathy

Author

Guo, Tai, primary, Liu, Yue, additional, He, Mengxia, additional, Lian, Yuling, additional, Wu, Xiyu, additional, Zhao, Hangpeng, additional, Chen, Xiaohong, additional, Huang, Xiaofeng, additional, Fang, Dejia, additional, Li, Yuman, additional, Mo, Zhixin, additional, Meng, Qianli, additional, Hou, Ning, additional, and Cui, Ying, additional

Published
2024
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15. High HDL-C and high LDL-C are risk factors of pterygium in a population-based cross-sectional study in Southern China: the Dongguan Eye Study

Author

Zang, Siwen, primary, Chen, Yanlei, additional, Guo, Haike, additional, Zhang, Min, additional, Zhang, Guanrong, additional, Zhang, Lixin, additional, Zhang, Liang, additional, Liu, Qingyang, additional, Zeng, Jin, additional, Lo, Chun-Han, additional, Cui, Ying, additional, and Meng, Qianli, additional

Published
2022
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23. A multi-task deep-learning system for assessment of diabetic macular ischemia on optical coherence tomography angiography images

Author

Yang, Dawei, primary, Sun, Zihan, additional, Shi, Jian, additional, Ran, Anran, additional, Tang, Fangyao, additional, Tang, Ziqi, additional, Lok, Jerry, additional, Szeto, Simon, additional, Chan, Jason, additional, Yip, Fanny, additional, Zhang, Liang, additional, Meng, Qianli, additional, Rasmussen, Martin, additional, Grauslund, Jakob, additional, and Cheung, Carol Y., additional

Published
2021
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28. The Global-First Topological Definition of Perceptual Objects, and Its Neural Correlation in Anterior Temporal Lobe

Author

Chen, Lin, Zhou, Ke, Qian, Wenli, Meng, Qianli, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Zhong, Ning, editor, Callaghan, Vic, editor, Ghorbani, Ali A., editor, and Hu, Bin, editor

Published
2011
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30. Prevalence and Pattern of Geographic Atrophy in Asia

Author

Rim, Tyler Hyungtaek, primary, Kawasaki, Ryo, additional, Tham, Yih-Chung, additional, Kang, Se Woong, additional, Ruamviboonsuk, Paisan, additional, Bikbov, Mukharram M., additional, Miyake, Masahiro, additional, Hao, Jie, additional, Fletcher, Astrid, additional, Sasaki, Mariko, additional, Nangia, Vinay, additional, Sabanayagam, Charumathi, additional, Yu, Marco, additional, Fujiwara, Kohta, additional, Thapa, Raba, additional, Wong, Ian Y., additional, Kayama, Takamasa, additional, Chen, Shih-Jen, additional, Kuang, Tung-Mei, additional, Yamashita, Hidetoshi, additional, Sundaresan, Periasamy, additional, Chan, Jonathan C., additional, van Rens, G.H.M.B., additional, Sonoda, Koh-Hei, additional, Wang, Ya Xing, additional, Panda-Jonas, Songhomitra, additional, Harada, Sei, additional, Kim, Ramasamy, additional, Ganesan, Suganeswari, additional, Raman, Rajiv, additional, Yamashiro, Kenji, additional, Gilmanshin, Timur R., additional, Jenchitr, Watanee, additional, Park, Kyu Hyung, additional, Gemmy Cheung, Chui Ming, additional, Wong, Tien Yin, additional, Wang, Ningli, additional, Jonas, Jost B., additional, Chakravarthy, Usha, additional, Cheng, Ching-Yu, additional, Yanagi, Yasuo, additional, Saenmee, Achareeya, additional, Cao, Kai, additional, George, Ronnie, additional, Kazakbaeva, Gyulli M., additional, Khalimov, Timur A., additional, Khanna, Rohit C., additional, Kim, Hyun Woong, additional, Kulothungan, Vaitheeswaran, additional, Nangia, Prabhat, additional, Mao, Feifei, additional, Matsuda, Fumihiko, additional, Meng, Qianli, additional, Namba, Hiroyuki, additional, Pokawattana, Nattapol, additional, Oh, Jaeryung, additional, Park, Sang Jun, additional, Ravindran, Ravilla, additional, Sharma, Tarun, additional, Shin, Jae Pil, additional, Surya, Janani, additional, Takahashi, Ayako, additional, Takebayashi, Toru, additional, Tsujikawa, Akitaka, additional, Vashist, Praveen, additional, Wei, Wen Bin, additional, Yang, Xiaohui, additional, Yu, Seung-Young, additional, Zainullin, Rinat M., additional, and Zhao, Pei Quan, additional

Published
2020
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32. Is kidney function associated with primary open-angle glaucoma? Findings from the Asian Eye Epidemiology Consortium

Author

Tham, Yih-Chung, primary, Tao, Yijin, additional, Zhang, Liang, additional, Rim, Tyler Hyung Taek, additional, Thakur, Sahil, additional, Lim, Zhi Wei, additional, Chee, Miao Li, additional, Bikbov, Mukharram M, additional, Kazakbaeva, Gyulli M, additional, Wang, Ningli, additional, Cao, Kai, additional, Hao, Jie, additional, Nangia, Vinay, additional, Panda-Jonas, Songhomitra, additional, Wang, Ya Xing, additional, Wong, Ian Y, additional, Chan, Jonathan Cheuk Hung, additional, Meng, Qianli, additional, Sabanayagam, Charumathi, additional, Wong, Tien Y, additional, Jonas, Jost B, additional, Zhong, Hua, additional, and Cheng, Ching-Yu, additional

Published
2020
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33. Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia

Author

Meng, Qianli, Qin, Yaowu, Deshpande, Monika, Kashiwabuchi, Fabiana, Rodrigues, Murilo, Lu, Qiaozhi, Ren, Hui, Elisseeff, Jennifer H., Semenza, Gregg L., Montaner, Silvia V., and Sodhi, Akrit

Subjects
pterygia, Vascular Endothelial Growth Factor A, Digoxin, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Pterygium, Real-Time Polymerase Chain Reaction, Transfection, angiogenesis, Angiopoietin-Like Protein 4, Animals, Humans, RNA, Messenger, hypoxia-inducible factor, Cells, Cultured, vascular endothelial growth factor, Neovascularization, Pathologic, angiopoietin-like 4, Angiogenesis Modulating Agents, Epithelial Cells, Hypoxia-Inducible Factor 1, alpha Subunit, eye diseases, Phenotype, Gene Expression Regulation, RNA Interference, Anatomy and Pathology/Oncology, Endothelium, Vascular, Rabbits, Angiopoietins, Conjunctiva
Abstract

Purpose Disappointing results from clinical studies assessing the efficacy of therapies targeting vascular endothelial growth factor (VEGF) for the treatment of pterygia suggest that other angiogenic mediators may also play a role in its development. We therefore explore the relative contribution of VEGF, hypoxia-inducible factor (HIF)-1α (the transcription factor that regulates VEGF expression in ocular neovascular disease), and a second HIF-regulated mediator, angiopoietin-like 4 (ANGPTL4), to the angiogenic phenotype of pterygia. Methods Expression of HIF-1α, VEGF, and ANGPTL4 were examined in surgically excised pterygia, and in immortalized human (ih) and primary rabbit (pr) conjunctival epithelial cells (CjECs). Endothelial cell (EC) tubule formation assays using media conditioned by ihCjECs in the presence or absence of inducers/inhibitors of HIF-1 or RNA interference (RNAi) targeting VEGF, ANGPTL4, or both were used to assess their relative contribution to the angiogenic potential of these cells. Results HIF-1α and VEGF expression were detected in 6/6 surgically excised pterygia and localized to CjECs. Accumulation of HIF-1α in was confirmed in ihCjECs and prCjECs, including stratified prCjECs grown on collagen vitrigel, and resulted in expression of VEGF and the promotion of EC tubule formation; the latter effect was partially blocked using RNAi targeting VEGF mRNA expression. We demonstrate expression of a second HIF-regulated angiogenic mediator, ANGPTL4, in CjECs in culture and in surgically excised pterygia. RNAi targeting ANGPTL4 inhibited EC tubule formation and was additive to RNAi targeting VEGF. Conclusions Our results support the development of therapies targeting both ANGPTL4 and VEGF for the treatment of patients with pterygia.

Published
2017

38. Synthesis, Characterization, and Evaluation of Near-IR Boron Dipyrromethene Bioconjugates for Labeling of Adenocarcinomas by Selectively Targeting the Epidermal Growth Factor Receptor

Author

Kaufman, Nichole E. M., primary, Meng, Qianli, additional, Griffin, Kaitlin E., additional, Singh, Sitanshu S., additional, Dahal, Achyut, additional, Zhou, Zehua, additional, Fronczek, Frank R., additional, Mathis, J. Michael, additional, Jois, Seetharama D., additional, and Vicente, M. Graça H., additional

Published
2019
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41. A MULTITASK DEEP-LEARNING SYSTEM FOR ASSESSMENT OF DIABETIC MACULAR ISCHEMIA ON OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY IMAGES

Author

Yang, Dawei, Sun, Zihan, Shi, Jian, Ran, Anran, Tang, Fangyao, Tang, Ziqi, Lok, Jerry, Szeto, Simon, Chan, Jason, Yip, Fanny, Zhang, Liang, Meng, Qianli, Rasmussen, Martin, Grauslund, Jakob, and Cheung, Carol Y.

Abstract

Supplemental Digital Content is Available in the Text.We developed and tested a deep-learning system that achieved good performance in performing image quality assessment as well as classifying the presence or absence of diabetic macular ischemia in both superficial and deep capillary plexuses in two widely used optical coherence tomography angiography devices for primary validation and external testing.

Published
2022
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42. Programmed Death 1 (PD-1) is involved in the development of proliferative diabetic retinopathy by mediating activation-induced apoptosis

Author

Fang, Mengyuan, Meng, Qianli, Guo, Haike, Wang, Liya, Zhao, Zhaoxia, Zhang, Liang, Kuang, Jian, Cui, Ying, Mai, Liping, and Zhu, Jiening

Subjects
Adult, Male, Diabetic Retinopathy, endocrine system diseases, Programmed Cell Death 1 Receptor, Apoptosis, Middle Aged, Ligands, Lymphocyte Activation, Real-Time Polymerase Chain Reaction, eye diseases, Case-Control Studies, Humans, Female, Lymphocytes, RNA, Messenger, Research Article, Aged
Abstract

Purpose Recent studies revealed that immunological mechanisms play a prominent role in the pathogenesis of proliferative diabetic retinopathy (PDR). Given the importance of the immune response in PDR and the significance of the programmed death 1 (PD-1) pathway as an immune regulatory pathway, the aim of this study is to determine the expression and functional characteristics of the PD-1 pathway in peripheral blood lymphocytes from patients with PDR. Methods Peripheral blood lymphocytes were obtained from patients with PDR, age-matched patients with diabetes mellitus and no diabetic retinopathy (DM-NDR), and controls. The mRNA expression of PD-1 and its ligands were determined using real-time PCR. The frequencies of PD-1 and its ligands, activation-induced apoptosis, IFN-γ, and IL-4 were determined by flow cytometry. Results The PD-1 mRNA expression markedly decreased, while the frequency of PD-1+ cells increased in the PDR group compared with the DM-NDR and control groups. The expression of PD-ligand 1 (PD-L1) mRNA and PD-L1+ cells in the PDR group was lower than that in the other two groups. In the PDR group, the frequency of Annexin V+PI- and Annexin V+PI-PD-1+ cells increased, while the frequency of Annexin V+PI-PD-L1+ cells decreased. Although their expression was upregulated, the ratio of PD-1+ IFN-γ+ to PD-1+IL-4+ cells in the PDR group was not significantly different to that in the DM-NDR and control groups. Conclusions These results suggest that PD-1 is involved in the development of PDR by mediating activation-induced apoptosis.

Published
2015

47. PDCD1 genes may protect against extraocular manifestations in Chinese Han patients with Vogt-Koyanagi-Harada syndrome

Author

Meng, Qianli, Liu, Xiaoli, Yang, Peizeng, Hou, Shengping, Du, Liping, Zhou, Hongyan, and Kijlstra, Aize

Subjects
Adult, Male, China, Chi-Square Distribution, Programmed Cell Death 1 Receptor, HLA-DR Antigens, Polymorphism, Single Nucleotide, eye diseases, Linkage Disequilibrium, Gene Frequency, Haplotypes, Antigens, CD, Case-Control Studies, HLA-DR4 Antigen, Humans, Female, Genetic Predisposition to Disease, Apoptosis Regulatory Proteins, Uveomeningoencephalitic Syndrome, HLA-DRB4 Chains, Polymorphism, Restriction Fragment Length, Research Article
Abstract

Purpose To analyze the potential association of programmed cell death 1 (PDCD1) with Vogt-Koyanagi-Harada (VKH) syndrome in a Chinese Han population. Methods Three single nucleotide polymorphism (SNPs), PD-1.3G/A, PD-1.5C/T, and PD-1.6G/A, were genotyped in 247 VKH patients and 289 age-, sex-, and ethnically-matched healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The associations of genotypes and alleles with VKH syndrome were analyzed. Results All genotype distributions in healthy controls were in Hardy–Weinberg equilibrium. The genotype and allele frequencies of PD-1.3, PD-1.5, and PD-1.6 were not different between patients with VKH syndrome and healthy controls. No significant difference was observed according to the status of human leukocyte antigen (HLA)-DR4 and HLA-DRw53. Compared to the controls, lower frequencies of the PD-1.5C genotype and allele frequencies were observed in VKH patients with extraocular findings. Conclusions PD-1.3 and PD-1.6 polymorphisms are not associated with the susceptibility to VKH syndrome in the Chinese Han population. However, PD-1.5 may be negatively associated with the occurrence of extraocular manifestations of VKH syndrome.

Published
2009

49. Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection

Author

Meng, Qianli, Yang, Peizeng, Jin, Haoli, Rosenbaum, James T., Li, Bing, Zhang, Haining, Zhou, Hongyan, Huang, Xiangkun, and Planck, Stephen R.

Subjects
Mice, Inbred BALB C, Microscopy, Confocal, Staining and Labeling, Ovalbumin, Corneal Stroma, Epithelium, Corneal, Antigen-Presenting Cells, Fluorescent Antibody Technique, eye diseases, Antibodies, Injections, Cornea, Vitreous Body, Mice, Microscopy, Electron, Phenotype, Microscopy, Fluorescence, Animals, Female, Tissue Distribution, sense organs, Antigens, Research Article
Abstract

Purpose To study the phenotypes, distribution, and morphologies of different antigen-presenting cells (APCs) in the murine cornea. Methods Intravitreal injection of fluorescently tagged ovalbumin (OVA) or antibodies to MHC-II (I-Ad), F4/80, CD11c, B7-1, and B7-2 was performed to label cells in the murine cornea. Light and transmission electron microscopy were used to examine corneal histology. Intravital microscopy, epifluorescence microscopy, and confocal microscopy were used to evaluate the labeled cells. In vitro staining was performed to validate the in vivo staining and localize the labeled cells. Three-dimensional rotatable images were taken to evaluate relationships between two differently labeled cells. Results Histological examination revealed no observable change in the cornea following intravitreal injection. In vivo staining showed that OVA+ cells and cells positive for MHC-II, F4/80, CD11c, B7-1, or B7-2 were noted throughout the cornea with a decreasing density from limbus toward the central cornea. Two populations with distinct morphological features were identified among these APCs. Labeled cells were found beneath the epithelium or in the shallow stroma in the central and paracentral cornea, but in all layers in the peripheral cornea. A number of F4/80+ and CD11c+ cells were also positive for OVA, MHC-II, B7-1, or B7-2. Rotatable images showed a close contact between two differently labeled cells. Conclusions Intravitreal injection of labeled antibodies can be adapted to visualize labeled cells in the cornea. APCs with distinct morphologies, phenotypes, and distribution may contribute to the immunologically privileged feature of the cornea.

Published
2007

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