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Summary Meloidogyne enterolobii (Me) is an emerging root-knot nematode (RKN) and the most important worldwide for its high aggressiveness and increasing geographical distribution. In Mexico only in watermelon and tomato has been reported. Here we report the presence of this nematode in Jalapeño pepper collected in the state of Sinaloa. The morphological and morphometric characteristics of the second-stage juveniles and adult females were similar to those described for Me. DNA sequencing and alignment analysis (BLAST/GenBank) showed that the SCAR and rDNA IGS2 fragments had a 99 % of identity, with Me DNA sequences present in the database. The results obtained indicate that the RKN detected corresponds to M. enterolobii. To our knowledge, this is the first report of this nematode in chilli pepper in this region of Mexico, considered of major economic importance for the variety of vegetables grown and the extension of the area sown.

Made available in DSpace on 2019-10-06T15:54:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Root-knot nematode Meloidogyne mayaguensis Rammah & Hirschmann is a limiting factor in guava tree (Psidium guajava L.) production around the world. An alternative to address this problem is the use of resistant root-knot nematode rootstocks. However, the guava nursery system which is based on herbaceous stem cutting propagation needs to be modified as a rootstock produced via cutting must be grafted with nematode susceptible commercial cultivars. Thus, the objective of this study was to verify the feasibility of omega bench grafting in guava tree propagated by herbaceous stem cutting. Root-knot nematode tolerant ‘Tailandesa’ herbaceous stem cuttings was used as rootstock and ‘Paluma’ and ‘Pedro Sato’ as commercial cultivar grafts. It was observed 100% rooting, cutting survival, and leaf retention on rootstocks grafted with ‘Paluma’ and ‘Pedro Sato’. However, grafting take was not successful as the tissues presented fast oxidation, dehydration and graft death in both commercial cultivars. However, the immersion of the grafts into a solution containing 2.0% citric acid and 1.0% ascorbic acid controlled the oxidation process and resulted in 28.6% and 37.5% successful grafting in the combinations ‘Paluma’/‘Tailandesa’ and ‘Pedro Sato’/’Tailandesa’, respectively. Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP)

Holo- and paratypes of the root-knot nematodes Meloidogyne mayaguensis Rammah & Hirschmann, 1988 and M. enterolobii Yang & Eisenback, 1983 were morphometrically and morphologically compared. All observed female, male and second-stage juvenile morphometrical and morphological characters are identical for the two studied species. Additionally, contradictions between the original species descriptions were unravelled.The present study of holo- and paratypes confirms the taxonomical status of Meloidogyne mayaguensis as a junior synonym for M. enterolobii.

In the present work, we report for the first time the occurence of M. enterolobii infecting Capsicum annuumplants carrying the Me1 and Me3/Me7 genes under plastic house systems in Brasília-DF (Central Brazil).

Meloidogyne enterolobii (= Meloidogyne mayaguensis) : a case study of a root-knot nematode species Meloidogyne enterolobii is currently seen as one of the upcoming plant parasitic nematodes. The progress of this nematode, first reported in 1991 from South Africa, from nearly forgotten to quarantine organism, is discussed.

Etude de trois souches d'Arthrobotrys oligospora : Caractérisation biologique et effets sur Meloidogyne mayaguensis parasite de la tomate au Sénégal. Three strains (ORS 18690 S2, ORS 18691 S6 and ORS 18693 S5) of the nematophagous fungus Arthrobotrys oligospora have been isolated in Senegal for the first time. In vitro, two strains (ORS 18690 S2 and ORS 18693 S5) of them trapped 100 % and the other (ORS 18691 S5) 80 %> of 7-day-old juvenile Meloidogyne mayaguensis within 48h. Optimal growth occured at 25-30°C and at a pH 5.6, but salinity inhibited development. In order to test the ability of fungi to control M. mayaguensis in pots on tomato, the fungus was incorporated into compost blocks or in vermiculite before sowing or subsequent transplanting. In pot experiments both strains reduced nematode populations ans stimulated seedling growth. However, these effects were higher in compost blocks than in vermiculite.

Two assays were conducted to assess the influence of infective juveniles (IJs) of Heterorhabditis baujardi LPP7 on the embryogenesis and hatching of Meloidogyne mayaguensis. In the first assay, eggs were incubated in water alone or in the presence of infective juveniles, and completion of embryogenesis was evaluated 14 days later. In the second assay, unhatched second-stage juveniles were incubated in distilled water alone or in the presence of infective juveniles. Cumulative hatching was compared at various time intervals. Embryogenesis was not affected, whereas second-stage juveniles hatching was delayed probably because of the eggs permeability to noxious metabolites released by Photorhabdus luminescens, which is the bacterial symbiont of H. baujardi.

Conselho Nacional de Desenvolvimento Científico e Tecnológico Considering the importance of Meloidogyne enterolobii (= M. mayaguensis) to the culture of guava and the potential of Essential Oil of Mustard (EOM) on nematode control, was aimed to evaluate the efficiency of EOM on control of these nematodes in the field, on a orchard of plants uprooted and cut off at 0.3 m from soil level or in intact plants in production. An orchard of guava trees naturally infested with M. enterolobii, in Viçosa, MG, was selected. EOM was applied on soil at 100 μg/mL, by strips of cardboard in the rhizosphere of intact plants, or by irrigating the cultivation area with EOM diluted in water after the plants have been cut off or uprooted. Samples were collected over 140 days after the product application and evaluated on the number of J2 in soil and number of galls and eggs in roots of tomato plants in the bioassay. The J2 population in soil underwent rapid reduction, regardless of treatment, and was configured as an unreliable variable. By the bioassay, was obtained a reduction of 71%, 81% and 99% for number of galls and 60%, 80% and 83% for number of eggs at 45, 75 and 140 days after application, respectively, for plots treated with EOM, in uprooted or cutted plants. The terbuphos only reduced the number of galls during 45 days. The biological evaluation at 140 days revealed a general trend of population reduction, considering the number of galls and eggs. In intact plants, there was no control of nematodes, as the average variations of J2, galls and eggs did not differ significantly between treatments. In conclusion, the EOM was effective in controlling M. enterolobii at the field in the absence of the host. Considerando a importância de Meloidogyne enterolobii (= M. mayaguensis) para a cultura da goiabeira e o potencial do óleo essencial de mostarda (OEM) no controle de nematoides, objetivou-se avaliar a eficiência do OEM no controle do nematoide no campo, em pomares de plantas arrancadas, recepadas ou em produção. Selecionou-se um pomar de goiabeiras naturalmente infestado por M. enterolobii, em Viçosa, MG. O OEM foi aplicado no solo a 100 μg/mL, por meio de tiras de papelão na rizosfera de plantas intactas ou irrigando a área de cultivo com OEM diluído em água, após as plantas serem recepadas ou arrancadas. Em amostras coletadas ao longo de 140 dias após aplicação do produto, avaliou-se o número de J2 no solo e número de galhas e ovos em raízes de tomateiros, no teste biológico. Como a população de J2 no solo sofreu rápida redução, independente do tratamento, ela foi considerada uma variável pouco confiável para a avaliação. Pelo teste biológico, obteve-se uma redução de 71%, 81% e 99% para número de galhas e 60%, 80% e 83% para número de ovos aos 45, 75 e 140 dias após a aplicação, respectivamente, para as parcelas tratadas com OEM, em plantas arrancadas ou recepadas. O terbufós reduziu apenas o número de galhas aos 45 dias, o qual voltou a aumentar posteriormente. Aos 140 dias, houve tendência generalizada de redução da população, considerando-se o número de galhas e ovos. Nas plantas intactas não houve controle do nematoide, pois as médias das variações de J2, galhas e ovos não diferiram significativamente entre os tratamentos. Em conclusão, o OEM foi eficiente em controlar M. enterolobii no campo na ausência do hospedeiro.

Made available in DSpace on 2014-06-11T19:28:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-01-29Bitstream added on 2014-06-13T18:34:57Z : No. of bitstreams: 1 westerich_jn_me_botfca.pdf: 758227 bytes, checksum: a20725396920cbb320adf36fe0a8bc3b (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A espécie Meloidogyne mayaguensis vem causando grande preocupação por ser capaz de vencer a resistência de tomateiros portadores do gene Mi. O objetivo do trabalho foi estudar o ciclo biológico e a histopatologia de M. mayaguensis em tomateiros resistentes a meloidoginose e compará-los com os aspectos biológicos e histopatológicos de Meloidogyne javanica nas mesmas plantas. O delineamento experimental utilizado foi inteiramente casualizado, sendo para o estudo do ciclo biológico composto de vinte tratamentos (dois porta-enxertos de tomateiro, ‘Magnet’ e ‘Helper M’; duas espécies de nematoides das galhas, M. mayaguensis e M. javanica; e cinco épocas de coleta, 3 DAI, 10 DAI, 17 DAI, 24 DAI e 31 DAI), com cinco repetições. Para o estudo da histopatologia, composto por 12 tratamentos (dois porta-enxertos de tomateiro, ‘Magnet’ e ‘Helper M’; duas espécies de nematoides das galhas, M. mayaguensis e M. javanica; e três épocas de coleta, 10 DAI, 17 DAI e 24 DAI), com três repetições. As plantas foram inoculadas com 500 juvenis infectivos (J2) de M. mayaguensis ou M. javanica. As avaliações foram realizadas em cinco épocas de coleta. Em cada coleta, cinco plantas foram retiradas ao acaso e suas raízes separadas em duas porções. Uma das partes foi submetida à coloração com Fucsina Ácida e dissecada sob microscópio estereoscópico para a contagem dos diferentes estádios de desenvolvimento dos nematoides. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Na outra porção do sistema radicular, foram feitos cortes histológicos transversais com 8 μm de espessura, corados com Floxina B seguido de Azul de Toluidina 0,05%, para determinação dos números e áreas das células gigantes e dos sítios de alimentação presentes no cilindro vascular da raiz. Os dados obtidos foram submetidos... The species Meloidogyne mayaguensis is causing great concern for being able to overcome the resistance of tomatoes carrying the Mi gene. The objective of this work was to study the life cycle and the histopathological changes of M. mayaguensis in nematode-resistant tomatoes and compare them with biological and histopathological aspects of Meloidogyne javanica in the same plants. The experimental design was completely randomized, and to study the life cycle composed of twenty treatments (two varieties of tomato, 'Magnet' and 'Helper M', two species of nematodes, M. javanica and M. mayaguensis and five harvest dates, 3 DAI, 10 DAI 17 DAI 24 DAI and 31 DAI), with five replicates. To study the histopathology, composed of 12 treatments (two varieties of tomato, 'Magnet' and 'Helper M', two species of nematodes, M. javanica and M. mayaguensis and three harvest dates, 10 DAI, 17 DAI and 24 DAI), with three replications. The plants were inoculated with 500 infective juveniles (J2) of M. mayaguensis or M. javanica. The evaluations were conducted in five sampling times. At each harvest, five plants were taken at random and their roots separated into two parts. The first was stained with Acid Fuchsin and dissected under a stereomicroscope to count the different developmental stages of nematodes. The data were subjected to analysis of variance and means compared by Tukey test at 5% probability. In the another portion of the root system, 8 microns thick transversal sections were obtained, stained with Phloxine B followed by Toluidine Blue 0.05%, for determining the numbers and areas of giant cells and feeding sites in the vascular cylinder. The data were subjected to Multivariate Cluster Analysis and Principal Component Analysis. The results showed that the species M. mayaguensis was able to develop normally in the root-stocks, completing its cycle 24 days after inoculation (DAI)... (Complete abstract click electronic access below)

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:47:05Z No. of bitstreams: 1 S0100-204X2009000400014.pdf: 175490 bytes, checksum: acfceb46883a729a9606d01f47ee7aa6 (MD5) Made available in DSpace on 2013-08-22T18:47:05Z (GMT). No. of bitstreams: 1 S0100-204X2009000400014.pdf: 175490 bytes, checksum: acfceb46883a729a9606d01f47ee7aa6 (MD5) Previous issue date: 2009-04-01 Made available in DSpace on 2013-09-30T19:36:46Z (GMT). No. of bitstreams: 2 S0100-204X2009000400014.pdf: 175490 bytes, checksum: acfceb46883a729a9606d01f47ee7aa6 (MD5) S0100-204X2009000400014.pdf.txt: 13130 bytes, checksum: 034b260cf3c87b8ef233185a0d4b9012 (MD5) Previous issue date: 2009-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:16:25Z No. of bitstreams: 2 S0100-204X2009000400014.pdf: 175490 bytes, checksum: acfceb46883a729a9606d01f47ee7aa6 (MD5) S0100-204X2009000400014.pdf.txt: 13130 bytes, checksum: 034b260cf3c87b8ef233185a0d4b9012 (MD5) Made available in DSpace on 2014-05-20T13:16:25Z (GMT). No. of bitstreams: 2 S0100-204X2009000400014.pdf: 175490 bytes, checksum: acfceb46883a729a9606d01f47ee7aa6 (MD5) S0100-204X2009000400014.pdf.txt: 13130 bytes, checksum: 034b260cf3c87b8ef233185a0d4b9012 (MD5) Previous issue date: 2009-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O objetivo deste trabalho foi identificar espécies de Myrtaceae resistentes a Meloidogyne mayaguensis. Acessos de araçazeiros e goiabeiras da Coleção de Plantas Frutíferas Nativas e Exóticas da Unesp/FCAV e acessos de araçazeiro de fragmentos de matas nativas do Nordeste do Estado de São Paulo e Triângulo Mineiro foram testados quanto à resistência ao nematódeo. As mudas receberam 4.000 ovos e juvenis de segundo estádio de M. mayaguensis por planta e foram conduzidas em casa de vegetação. Aos 150 dias, os genótipos foram avaliados quanto à resistência ao nematódeo com base no fator de reprodução. Três acessos de Psidium e um de Eugenia foram resistentes a M. mayaguensis. The objective this work was to identify Myrtaceae species resistant to Meloidogyne mayaguensis. Araça and guava accessions obtained from the Collection of Native and Exotic Fruitful Plants of Unesp/FCAV, and accessions of araça plant obtained from native forest fragments located in the northeast region of the São Paulo state and the Triângulo Mineiro region, Minas Gerais state, Brazil, were tested for resistance to M. mayaguensis. The seedlings received 4,000 eggs and second stadium juveniles of M. mayaguensis each and were reared in a greenhouse. After 150 days, the genotypes were evaluated for resistance to the nematode based on the reproduction factor. Three Psidium spp. and one Eugenia accessions were resistant to M. mayaguensis. Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias

The root-knot nematode, Meloidogyne mayaguensis, causes serious economic loss to guava plantation in Brazil. Fungi associated with eggs of M. mayaguensis in Brazilian soil have not been studied yet, but this knowledge could form the basis for future field studies on biological control of this nematode. The egg parasitic fungi, Pochonia chlamydosporia var. chlamydosporia, P.chlamydosporia var. catenulata and Lecanicillium psalliotae, were isolated from M. mayaguensis eggs and analyzed based on their cultural and morphological characteristics. The optimum temperature for growth and spore production ranged between 24 and 28oC for P. chlamydosporia isolates. For the isolate of L. psalliotae, the best growth was at 30 o C. In solid state fermentation, P. chlamydosporia isolates produced between 3.5 and 5.2 x 106 chlamydospores per g of colonized substrate, whereas L. psalliotae isolate produced 8.5 x 108 conidia per g of colonized substrate. Future studies are required to clarify the potential of these fungi as biological control agents of M. mayaguensis.

Em 2001, Meloidogyne mayaguensis foi identificado como o agente causal de drástica destruição de goiabeiras irrigadas no Vale do São Francisco. Algumas espécies vegetais são conhecidas como hospedeiras desse nematóide, especialmente aquelas de interesse comercial. No entanto, pouco se conhece ainda acerca do comportamento de espécies de plantas daninhas. Em uma área cultivada com goiabeiras, no município de Petrolina - PE, galhas características da infecção de Meloidogyne foram observadas nas raízes de maxixe (Cucumis anguria), apaga-fogo (Alternanthera tenella), jitirana-cabeluda (Merremia aegyptia) e meloso-da-flor-roxal (Marsypianthes chamaedrys). Em todas as plantas, M. mayaguensis foi identificado segundo o fenótipo da isoenzima esterase (Est M2, Rm: 0,7 e 0,9). Esse fato demonstra que plantas daninhas podem constituir eficiente reservatório do nematoide em áreas cultivads. Dessa maneira, na adoção de medidas de controle como a rotação de culturas ou o alqueive, periodicamente, essas plantas invasoras devem ser eliminadas das áreas a serem manejadas. Made available in DSpace on 2015-10-23T03:15:24Z (GMT). No. of bitstreams: 1 ID37202.pdf: 385663 bytes, checksum: 2ca1ba58fb43ec2b4d5ba647a47b28bc (MD5) Previous issue date: 2008-03-18 Edição dos Resumos do XXVII Congresso Brasileiro de Nematologia, Goiânia, maio 2007.

The in vitro paralysis of second-stage juveniles of Meloidogyne incognita, M. javanica and M. mayaguensis by aqueous extracts of 15 West African Crotalaria species was analysed. A multivariate analysis distinguished four groups, based on their nematostatic activity: i) C. glaucoides extracts were not active; ii) the root extracts from C. goreensis, C. lathyroides and C. perrottetii were more active than the shoot extracts; iii) the shoot extracts from C. comosa and C. cylindrocarpa were more active than the root extracts; iv) both shoot and root extracts of C. atrorubens, C. barkae, C. grantiana, C. hyssopifolia, C. pallida, C. podocarpa, C. retusa, C. senegalensis and C. sphaerocarpa have nematostatic effects depending on either the Crotalaria species or plant tissue from which the extracts were derived. However, considering the greater biomass contribution of the leaves and stems compared to the roots when the plants are used as green manure, C. barkae, C. grantiana, C. pallida and C. podocarpa are the most efficient Crotalaria species, whatever the Meloidogyne species targeted.

Meloidogyne mayaguensis foi assinalada pela primeira vez no Brasil em Petrolina (PE) e Curaca e Manicoba (BA), causando danos em plantios comerciais de goiabeira. As plantas infestadas pelo nematoide apresentaram drastica reducao de crescimento, folhas pequenas e reducao de producao em volume e em quantidade. Plantas severamente atacadas pelo nematoide apresentaram sistema radicular mal desenvolvido, deformado por muitas galhas de dimensoes variadoa e desprovidos de raizes finas. O nematoide esta provavelmente sendo disseminado com mudas provenientes de viveiros infestados. M. mayaguensis foi identificada e caracterizada atraves do uso de fenotipos enzimaticos e caracteres morfologicos e morfometricos. Made available in DSpace on 2018-09-26T00:38:57Z (GMT). No. of bitstreams: 1 NematologiaBrasileirav.25n.2p.2232282001.pdf: 4530244 bytes, checksum: 651ead591c03e7a2bf8435a5fc0a277e (MD5) Previous issue date: 2002-06-05

Meloidogyne mayaguensis on Psidium guajava in Rio Grande doNorte This paper reports the occurrence of Meloidogynemayaguensis on Psidium guajava cv. Paluma in the State of RioGrande do Norte, Brazil. O Laboratorio de Agricultura Irrigada da Escola Superiorde Agricultura de Mossoro – ESAM, Rio Grande do Norte,recebeu, em junho de 2003, amostras de solo de rizosfera degoiabeira ( Psidium guajava L.) cv. Paluma e sistemas radicularesdeficientes constituidos por poucas raizes, finas e necrosadas,coletadas em pomar com aproximadamente 500 plantas, comsete anos de idade, situado no municipio de Touros–RN.Segundo o proprietario, as plantas doentes expressavamsintomas de amarelecimento e declinio, e distribuiam-se emreboleiras.Para diagnostico, amostras foram encaminhadas aoLaboratorio de Fitonematologia da Universidade Federal Ruralde Pernambuco; das quais aliquotas de solo de 300 cm 3 foramdestinadas ao processamento por flotacao centrifuga, segundoJenkins (1964) (Plant Disease Reporter, v.48, p.692, 1964), e 10g de raizes para trituracao em liquidificador, por 20 s, associadaa flotacao centrifuga. Nas amostras de solo e de raiz foramdetectadas 440 e 11.380 formas juvenis do segundo estadio,respectivamente, de