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A green deposition method of zinc oxide nanoparticles using coffee leaf extraction was successfully prepared. The use of these preparation techniques is accepted by many researchers because it is nonexpensive and simple and has no environmental impact during the operation. The determination and reduction of Zn ions to ZnO NPs were characterized by using a UV-visible spectroscope. The UV-visible spectroscopy result reveals that the large band gap energy is observed in the visible region at the wavelength of 300 nm. X-ray diffraction and SEM analysis confirm that the deposited nanoparticle is highly crystalline with (111), (222), and (100) planes and cubic shape structure. The coffee leaf extraction serves as a reducing agent for stability of the particle length, where its medicinal value outcome showed an important antibacteria of the pathogenic type which appeared on the wound. The present research deals with the green synthesis of ZnO NPs as well as its application in toxicity reduction.