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156 results on '"Meistertzheim, Anne‐Leila"'

8. Comparison of the macro-, meso- and microplastic pollution in French riverbanks and beaches using citizen science with schoolchildren

Author

Philip, Léna, primary, Picard, Maela Le, additional, Lavergne, Edouard, additional, Bourgain, Pascaline, additional, Sabard, Brigitte, additional, Troublé, Romain, additional, Meistertzheim, Anne-Leila, additional, Ludwig, Wolfgang, additional, Halle, Alexandra ter, additional, Lacroix, Camille, additional, and Ghiglione, Jean-Francois, additional

Published
2024
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12. Marine Biodegradation of Tailor-Made Polyhydroxyalkanoates (Pha) Influenced by the Chemical Structure and Associated Bacterial Communities

Author

Ghiglione, Jean-François, primary, Philip, Léna, additional, lemechko, pierre, additional, EYHERAGUIBEL, Boris, additional, Meistertzheim, Anne-Leila, additional, Mireille, Pujo-Pay, additional, conan, pascal, additional, Barbe, Valérie, additional, Bruzaud, Stéphane, additional, and Derippe, Gabrielle, additional

Published
2023
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13. Biodegradability under marine conditions of bio-based and petroleum-based polymers as substitutes of conventional microparticles

Author

Cheng, Jingguang, primary, Eyheraguibel, Boris, additional, Jacquin, Justine, additional, Pujo-Pay, Mireille, additional, Conan, Pascal, additional, Barbe, Valérie, additional, Hoypierres, Julia, additional, Deligey, Gaëlle, additional, Halle, Alexandra Ter, additional, Bruzaud, Stéphane, additional, Ghiglione, Jean-François, additional, and Meistertzheim, Anne-Leila, additional

Published
2022
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14. Evidence of coupled autotrophy and heterotrophy on plastic biofilms and its influence on surrounding seawater

Author

Conan, Pascal, primary, Philip, Léna, additional, Ortega-Retuerta, Eva, additional, Odobel, Charlène, additional, Duran, Clélia, additional, Pandin, Caroline, additional, Giraud, Carolane, additional, Meistertzheim, Anne-Leila, additional, Barbe, Valérie, additional, Ter Hall, Alexandra, additional, Pujo-Pay, Mireille, additional, and Ghiglione, Jean-François, additional

Published
2022
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16. Microbial biodegradation of polyhydroxyalkanoates in the marine environment

Author

Philip, Léna, Odobel, Charlene, Jacquin, Justine, Derippe, Gabrielle, Villar, Emilie, Barbe, Valérie, Bruzaud, Stéphane, Meistertzheim, Anne-Leila, and Ghiglione, Jean Francois

Subjects
polyhydroxyalkanoates, DNA, biodegradation, stable isotope probing, plastisphere
Abstract

Among actual bioplastics, polyhydroxyalkanoates (PHA) has raised great interest as a substitute to persistent petroleum based plastic materials. Naturally produced by numerous bacteria as a form of carbon storage, PHA show good signs of biodegradability in natural ecosystems. Here, we evaluate the influence of the chemical structure (eight formulations of short and medium-chain-length PHA) on the biodegradability of PHA in the marine environment. We used an original miniaturized experimental design that allowed transdisciplinary analyses (dissolved inorganic carbon production, oxygen consumption, heterotrophic bacterial activity, microbial biomass and diversity, molecular weight, production of oligomers and monomers). After 30 days of incubation with a natural community, we observed different signs of biodegradability for the eight PHA. In particular, higher microbial activity was monitored in short-chain-length (scl) sample than in medium-chain-length (mcl). To better understand scl-PHA biodegradation by marine microbes, radio-labeled 13C-PHB was incubated with natural communities for 90 days. Microbial activity and biomass were monitored, and DNA was extracted on days 5, 13, 30 and 90 of incubation. DNA-Stable isotope probing (DNA-SIP) was used in order to describe degrading and opportunist communities. Metabarcoding analyses highlighted taxonomic differences between 13C and 12C fractions, and metagenomic analyses allowed us to decipher the functional difference between the two fractions. Also see: https://micro2022.sciencesconf.org/427467/document, In MICRO 2022, Online Atlas Edition: Plastic Pollution from MACRO to nano

Published
2022
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17. New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach

Author

Meistertzheim, Anne-Leila, Calves, Isabelle, Roussel, Valerie, Van Wormhoudt, Alain, Laroche, Jean, Huchette, Sylvain, and Paillard, Christine

Subjects
Genetic markers -- Research, Abalones -- Genetic aspects, Vibrio infections -- Genetic aspects, Biological sciences
Abstract

Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. numerous episodic mass mortalities of the abalone Haliotis tuberculata have been [...]

Published
2014
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18. Complex genetic structure of a euryhaline marine fish in temporarily open/closed estuaries from the wider Gulf of Aden

Author

Lavergne, Edouard, Calves, Isabelle, Meistertzheim, Anne Leila, Charrier, Gregory, Zajonz, Uwe, and Laroche, Jean

Subjects
Gulf of Aden -- Environmental aspects, Fish populations -- Genetic aspects, Biological sciences
Abstract

Temporarily open/closed estuaries (TOCEs) are major ecosystems of the Indian Ocean coastal zones. Their functioning is tightly linked to climatic events such as monsoons and storms, and their mouth can close up for prolonged and variable periods of time, thus limiting their connectivity with the marine environment. Two types of genetic markers (i.e., mitochondrial cytochrome c oxidase I (COI) gene and microsatellites) were used to assess the genetic structure of 288 individuals of Terapon jarbua, a widely distributed fish species in the wider gulf of Aden. Firstly, the hypothesis of panmixia was tested. Then, alternative hypotheses were investigated to explain the population genetic structure of T. jarbua: could it be shaped by (1) regional biogeographic barriers (i.e., Socotra Island vs. mainland Yemen) and/or (2) the particular functioning of TOCEs in relation to the species life cycle and particular physical ocean parameters? On one hand, the polymorphism of the COI inferred (1) a high haplotype diversity and a reduced nucleotide diversity over the whole data set and (2) a 'star-like' shape of the haplotype network, thus suggesting a population expansion after local extinctions during the Pleistocene glaciations. On a second hand, the genotyping of eight microsatellites showed a significant genetic differentiation between T. jarbua populations in the wider Gulf of Aden ([F.sub.ST] = 0.035, p < 0.01), and thus, the panmixia hypothesis was rejected. Analyses of molecular variance results did not show any significant structure between Socotra Island and mainland Yemen and thus did not support the role of biogeographic barriers in structuring T. jarbua populations. Significant multi-locus deficits in heterozygotes at particular locations displaying high levels of [F.sub.IS] were recorded. It was suggested that a possible Wahlund effect took place in those TOCEs which could gather several cohorts of larvae stemming from different marine subpopulations over the sampled area. The present study emphasized the uniqueness of each TOCE as a potential reservoir of biodiversity and the urgent need for a better conservation program of those estuaries in the region, in order to avoid habitat fragmentation and permanent closure of those nursery areas by human activities., Introduction The functioning of temporarily open/closed estuaries (TOCEs) is tightly linked to climatic events such as monsoons in the Indian Ocean. In relation to specific physical conditions (ebb-tidal flow and [...]

Published
2014
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19. Bacterial Abundance, Diversity and Activity During Long-Term Colonization of Non-biodegradable and Biodegradable Plastics in Seawater

Author

Odobel, Charlene, primary, Dussud, Claire, additional, Philip, Lena, additional, Derippe, Gabrielle, additional, Lauters, Marion, additional, Eyheraguibel, Boris, additional, Burgaud, Gaëtan, additional, Ter Halle, Alexandra, additional, Meistertzheim, Anne-Leila, additional, Bruzaud, Stephane, additional, Barbe, Valerie, additional, and Ghiglione, Jean-Francois, additional

Published
2021
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20. Genetic structure of wild European populations of the invasive Pacific oyster Crassostrea gigas due to aquaculture practices

Author

Meistertzheim, Anne-Leila, Arnaud-Haond, Sophie, Boudry, Pierre, and Thebault, Marie-Therese

Subjects
Crassostrea -- Genetic aspects -- Distribution, Animal introduction -- Genetic aspects -- Distribution, Oysters -- Genetic aspects -- Distribution, Company distribution practices, Biological sciences
Abstract

As a result of aquaculture activities, Pacific oysters Crassostrea gigas (Thunberg, 1793) have invaded European coasts. Using seven microsatellites, we found virtually no genetic differentiation between natural populations throughout the European range (from the south of the Wadden Sea (the Netherlands) to the south of France) and French cultivated oysters. The genetic homogeneity of Pacific oyster samples appears to be the result of repeated transfers from same seed stocks made for aquaculture and, to a lesser extent, widespread dispersal due to specific biological traits of this species. The only genetic differentiation of Sylt population in the north of the Wadden Sea (Germany) suggests a stronger, persistent impact of ongoing supply of new genetic material from hatchery production, corresponding to seeds selection made by breeders. All of our genetic data highlighted the importance of aquaculture practices on the genetic structure of the keystone invader C. gigas in Europe., Introduction Marine ecosystems have always been subject to changes in species composition due to natural migration and can be favored by climatic variations. For several decades, the impact of human [...]

Published
2013
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21. Microbial Diversity and Activity During the Biodegradation in Seawater of Various Substitutes to Conventional Plastic Cotton Swab Sticks

Author

Jacquin, Justine, primary, Callac, Nolwenn, additional, Cheng, Jingguang, additional, Giraud, Carolane, additional, Gorand, Yonko, additional, Denoual, Clement, additional, Pujo-Pay, Mireille, additional, Conan, Pascal, additional, Meistertzheim, Anne-Leila, additional, Barbe, Valerie, additional, Bruzaud, Stéphane, additional, and Ghiglione, Jean-François, additional

Published
2021
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22. Relative Influence of Plastic Debris Size and Shape, Chemical Composition and Phytoplankton-Bacteria Interactions in Driving Seawater Plastisphere Abundance, Diversity and Activity

Author

Cheng, Jingguang, Jacquin, Justine, Conan, Pascal, Pujo-Pay, Mireille, Barbe, Valérie, George, Matthieu, Fabre, Pascale, Bruzaud, Stéphane, Ter Halle, Alexandra, Meistertzheim, Anne-Leila, Ghiglione, Jean-François, Laboratoire d'Océanographie Microbienne (LOMIC), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Dupuy de Lôme (IRDL), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Centre National de la Recherche Scientifique (CNRS), Interactions moléculaires et réactivité chimique et photochimique (IMRCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts (FERMAT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), SMODD - Systèmes Moléculaires Organisés et Développement Durable (SMODD), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie de Toulouse (ICT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées

Subjects
fungi, lcsh:QR1-502, colonization, Microbiology, lcsh:Microbiology, biofilm, microbial ecotoxicology, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, biofouling, plastic litter, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, plastisphere, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography, Original Research
Abstract

International audience; The thin film of life that inhabits all plastics in the oceans, so-called “plastisphere,” has multiple effects on the fate and impacts of plastic in the marine environment. Here, we aimed to evaluate the relative influence of the plastic size, shape, chemical composition, and environmental changes such as a phytoplankton bloom in shaping the plastisphere abundance, diversity and activity. Polyethylene (PE) and polylactide acid (PLA) together with glass controls in the forms of meso-debris (18 mm diameter) and large-microplastics (LMP; 3 mm diameter), as well as small-microplastics (SMP) of 100 μm diameter with spherical or irregular shapes were immerged in seawater during 2 months. Results of bacterial abundance (confocal microscopy) and diversity (16S rRNA Illumina sequencing) indicated that the three classical biofilm colonization phases (primo-colonization after 3 days; growing phase after 10 days; maturation phase after 30 days) were not influenced by the size and the shape of the materials, even when a diatom bloom (Pseudo-nitzschia sp.) occurred after the first month of incubation. However, plastic size and shape had an effect on bacterial activity (3H leucine incorporation). Bacterial communities associated with the material of 100 μm size fraction showed the highest activity compared to all other material sizes. A mature biofilm developed within 30 days on all material types, with higher bacterial abundance on the plastics compared to glass, and distinct bacterial assemblages were detected on each material type. The diatom bloom event had a great impact on the plastisphere of all materials, resulting in a drastic change in diversity and activity. Our results showed that the plastic size and shape had relatively low influence on the plastisphere abundance, diversity, and activity, as compared to the plastic composition or the presence of a phytoplankton bloom.

Published
2021

23. Microbial Diversity and Activity During the Biodegradation in Seawater of Various Substitutes to Conventional Plastic Cotton Swab Sticks

Author

Jacquin, Justine, Callac, Nolwenn, Cheng, Jingguang, Giraud, Carolane, Gorand, Yonko, Denoual, Clement, Pujo-pay, Mireille, Conan, Pascal, Meistertzheim, Anne-leila, Barbe, Valerie, Bruzaud, Stéphane, Ghiglione, Jean-françois, Jacquin, Justine, Callac, Nolwenn, Cheng, Jingguang, Giraud, Carolane, Gorand, Yonko, Denoual, Clement, Pujo-pay, Mireille, Conan, Pascal, Meistertzheim, Anne-leila, Barbe, Valerie, Bruzaud, Stéphane, and Ghiglione, Jean-françois

Abstract

The European Parliament recently approved a new law banning single-use plastic items for 2021 such as plastic plates, cutlery, straws, cotton swabs, and balloon sticks. Transition to a bioeconomy involves the substitution of these banned products with biodegradable materials. Several materials such as polylactic acid (PLA), polybutylene adipate terephthalate (PBAT), poly(butylene succinate) (PBS), polyhydroxybutyrate-valerate (PHBV), Bioplast, and Mater-Bi could be good candidates to substitute cotton swabs, but their biodegradability needs to be tested under marine conditions. In this study, we described the microbial life growing on these materials, and we evaluated their biodegradability in seawater, compared with controls made of non-biodegradable polypropylene (PP) or biodegradable cellulose. During the first 40 days in seawater, we detected clear changes in bacterial diversity (Illumina sequencing of 16S rRNA gene) and heterotrophic activity (incorporation of 3H-leucine) that coincided with the classic succession of initial colonization, growth, and maturation phases of a biofilm. Biodegradability of the cotton swab sticks was then tested during another 94 days under strict diet conditions with the different plastics as sole carbon source. The drastic decrease of the bacterial activity on PP, PLA, and PBS suggested no bacterial attack of these materials, whereas the bacterial activity in PBAT, Bioplast, Mater-Bi, and PHBV presented similar responses to the cellulose positive control. Interestingly, the different bacterial diversity trends observed for biodegradable vs. non-biodegradable plastics allowed to describe potential new candidates involved in the degradation of these materials under marine conditions. This better understanding of the bacterial diversity and activity dynamics during the colonization and biodegradation processes contributes to an expanding baseline to understand plastic biodegradation in marine conditions and provide a foundation for fu

Published
2021
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24. Diet shapes cold‐water corals bacterial communities

Author

Galand, Pierre E., primary, Remize, Marine, additional, Meistertzheim, Anne‐Leila, additional, Pruski, Audrey M., additional, Peru, Erwan, additional, Suhrhoff, Tim Jesper, additional, Le Bris, Nadine, additional, Vétion, Gilles, additional, and Lartaud, Franck, additional

Published
2019
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26. Out of the Mediterranean? Post‐glacial colonization pathways varied among cold‐water coral species

Author

Boavida, Joana, Becheler, Ronan, Choquet, Marvin, Frank, Norbert, Taviani, Marco, Bourillet, Jean-francois, Meistertzheim, Anne‐leila, Grehan, Anthony, Savini, Alessandra, Arnaud-haond, Sophie, Boavida, Joana, Becheler, Ronan, Choquet, Marvin, Frank, Norbert, Taviani, Marco, Bourillet, Jean-francois, Meistertzheim, Anne‐leila, Grehan, Anthony, Savini, Alessandra, and Arnaud-haond, Sophie

Abstract

Aim To infer cold‐water corals’ (CWC) post‐glacial phylogeography and assess the role of Mediterranean Sea glacial refugia as origins for the recolonization of the northeastern Atlantic Ocean. Location Northeastern Atlantic Ocean and Mediterranean Sea. Taxon Lophelia pertusa, Madrepora oculata. Methods We sampled CWC using remotely operated vehicles and one sediment core for coral and sediment dating. We characterized spatial genetic patterns (microsatellites and a nuclear gene fragment) using networks, clustering and measures of genetic differentiation. Results Inferences from microsatellite and sequence data were congruent, and showed a contrast between the two CWC species. Populations of L. pertusa present a dominant pioneer haplotype, local haplotype radiations and a majority of endemic variation in lower latitudes. Madrepora oculata populations are differentiated across the northeastern Atlantic and genetic lineages are poorly admixed even among neighbouring sites. Conclusions Our study shows contrasting post‐glacial colonization pathways for two key habitat‐forming species in the deep sea. The CWC L. pertusa has likely undertaken a long‐range (post‐glacial) recolonization of the northeastern Atlantic directly from refugia located along southern Europe (Mediterranean Sea or Gulf of Cadiz). In contrast, the stronger genetic differentiation of M. oculata populations mirrors the effects of long‐term isolation in multiple refugia. We suggest that the distinct and genetically divergent, refugial populations initiated the post‐glacial recolonization of the northeastern Atlantic margins, leading to a secondary contact in the northern range and reaching higher latitudes much later, in the late Holocene. This study highlights the need to disentangle the influences of present‐day dispersal and evolutionary processes on the distribution of genetic polymorphisms, to unravel the influence of past and future environmental changes on the connectivity of cosmopolitan deep‐sea

Published
2019
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30. Oxidative stress induced by glyphosate-based herbicide on freshwater turtles

Author

Héritier , Laurent, Duval , David, Galinier , Richard, Meistertzheim , Anne-Leila, Verneau , Olivier, Centre de Formation et de Recherche sur les Environnements Méditérranéens ( CEFREM ), Université de Perpignan Via Domitia ( UPVD ) -Centre National de la Recherche Scientifique ( CNRS ), Interactions Hôtes-Pathogènes-Environnements ( IHPE ), Université de Perpignan Via Domitia ( UPVD ) -Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre de Formation et de Recherche sur les Environnements Méditérranéens (CEFREM), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Interactions Hôtes-Pathogènes-Environnements (IHPE), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD)

Subjects
[ SDV.BID.EVO ] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Oxidative stress, Mauremys leprosa, Glyphosate Catalase, Superoxide dismutase, Gene expression, Enzyme activity, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, ComputingMilieux_MISCELLANEOUS, Trachemys scripta elegans
Abstract

International audience; Freshwater ecosystems face very strong anthropogenic pressures, among which overexploitation, habitat degradation, flow modification, species invasion, and water pollution lead to growing threats on biodiversity. Urbanization through wastewater treatment, industry through the release of inorganic and organic chemicals, and agriculture through the use of pesticides and herbicides are the main factors involved in water pollution. In France, more precisely in the Pyrenees-Orientales department, the poor quality of the watercourses is attributable overall to the use of glyphosate-based herbicides in agricultural activities. Because these chemicals can impact individuals, populations, and biodiversity, we investigated, under experimental conditions, the physiological response of animals facing abiotic contaminants. We selected as a model, juveniles of the freshwater turtle Trachemys scripta elegans. We measured the gene expression and activity of the catalase and superoxide dismutase enzymes as well as the levels of lipid peroxidation, which are all oxidative stress biomarkers, in turtles challenged with high concentrations of glyphosate-based herbicides, on the one hand, and with degraded waters collected from a local watercourse, on the other. We also measured the acetylcholinesterase activity across the same animals. We showed through variations in gene expression and enzyme activity that a glyphosate commercial formulation induced a stress in turtles. A similar outcome was obtained when turtles faced degraded waters. The results indicated that the poor quality of regional waters could be a real threat for animal health. Because turtles are globally less sensitive to contaminants than amphibians, which are lacking in the degraded waters of the Pyrenees-Orientales department, they could constitute an excellent model to follow the evolution of water quality through the study of oxidative stress biomarkers.

Published
2017

31. Colonization of Non-biodegradable and Biodegradable Plastics by Marine Microorganisms

Author

Dussud, Claire, Hudec, Cindy, George, Matthieu, Fabre, Pascale, Higgs, Perry, Bruzaud, Stephane, Delort, Anne-marie, Eyheraguibel, Boris, Meistertzheim, Anne-leila, Jacquin, Justine, Cheng, Jingguang, Callac, Nolwenn, Odobel, Charlene, Rabouille, Sophie, Ghiglione, Jean-frangois, Dussud, Claire, Hudec, Cindy, George, Matthieu, Fabre, Pascale, Higgs, Perry, Bruzaud, Stephane, Delort, Anne-marie, Eyheraguibel, Boris, Meistertzheim, Anne-leila, Jacquin, Justine, Cheng, Jingguang, Callac, Nolwenn, Odobel, Charlene, Rabouille, Sophie, and Ghiglione, Jean-frangois

Abstract

Plastics are ubiquitous in the oceans and constitute suitable matrices for bacterial attachment and growth. Understanding biofouling mechanisms is a key issue to assessing the ecological impacts and fate of plastics in marine environment. In this study, we investigated the different steps of plastic colonization of polyolefin-based plastics, on the first one hand, including conventional low-density polyethylene (PE), additivated PE with pro-oxidant (OXO), and artificially aged OXO (AA-OXO); and of a polyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), on the other hand. We combined measurements of physical surface properties of polymers (hydrophobicity and roughness) with microbiological characterization of the biofilm (cell counts, taxonomic composition, and heterotrophic activity) using a wide range of techniques, with some of them used for the first time on plastics. Our experimental setup using aquariums with natural circulating seawater during 6 weeks allowed us to characterize the successive phases of primo-colonization, growing, and maturation of the biofilms. We highlighted different trends between polymer types with distinct surface properties and composition, the biodegradable AA-OXO and PHBV presenting higher colonization by active and specific bacteria compared to non-biodegradable polymers (PE and OXO). Succession of bacterial population occurred during the three colonization phases, with hydrocarbonoclastic bacteria being highly abundant on all plastic types. This study brings original data that provide new insights on the colonization of non-biodegradable and biodegradable polymers by marine microorganisms.

Published
2018
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32. Diet shapes cold‐water corals bacterial communities.

Author

Galand, Pierre E., Remize, Marine, Meistertzheim, Anne‐Leila, Pruski, Audrey M., Peru, Erwan, Suhrhoff, Tim Jesper, Le Bris, Nadine, Vétion, Gilles, and Lartaud, Franck

Subjects
DEEP-sea corals, BACTERIAL communities, CORAL communities, LOPHELIA pertusa, ANIMAL nutrition
Abstract

Summary: Different cold‐water coral (CWC) species harbour distinct microbial communities and the community composition is thought to be linked to the ecological strategies of the host. Here we test whether diet shapes the composition of bacterial communities associated with CWC. We compared the microbiomes of two common CWC species in aquaria, Lophelia pertusa and Madrepora oculata, when they were either starved, or fed respectively with a carnivorous diet, two different herbivorous diets, or a mix of the 3. We targeted both the standing stock (16S rDNA) and the active fraction (16S rRNA) of the bacterial communities and showed that in both species, the corals' microbiome was specific to the given diet. A part of the microbiome remained, however, species‐specific, which indicates that the microbiome's plasticity is framed by the identity of the host. In addition, the storage lipid content of the coral tissue showed that different diets had different effects on the corals' metabolisms. The combined results suggest that L. pertusa may be preying preferentially on zooplankton while M. oculata may in addition use phytoplankton and detritus. The results cast a new light on coral microbiomes as they indicate that a portion of the CWC's bacterial community could represent a food influenced microbiome. [ABSTRACT FROM AUTHOR]

Published
2020
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36. Patterns of bacteria-host associations suggest different ecological strategies between two reef building cold-water coral species

Author

Meistertzheim, Anne. -leila, Lartaud, Franck, Arnaud-haond, Sophie, Kalenitchenko, Dimitri, Bessalam, Manon, Le Bris, Nadine, Galand, Pierre E., Meistertzheim, Anne. -leila, Lartaud, Franck, Arnaud-haond, Sophie, Kalenitchenko, Dimitri, Bessalam, Manon, Le Bris, Nadine, and Galand, Pierre E.

Abstract

Cold-water corals (CWC) are main ecosystem engineers of the deep sea, and their reefs constitute hot-spots of biodiversity. However, their ecology remains poorly understood, particularly, the nature of the holobiont formed by corals with their associated bacterial communities. Here, we analysed Madrepora oculata and Lophelia pertusa samples, collected from one location in a Mediterranean canyon in two different seasons (autumn and spring), in order to test for species specificity and temporal stability of the host-bacteria associations. The 16S rRNA sequencing revealed host-specific patterns of bacterial communities associated with L. pertusa and M. oculata, both in terms of community composition and diversity. All analyzed M. oculata polyps exhibited temporally and spatially similar bacterial communities dominated by haplotypes homologous to the known cnidarians-associated genus Endozoicomonas. In contrast, the bacterial communities associated with L. pertusa varied among polyps from the same colony, as well as among distinct colonies and between seasons. While the resilient consortium formed by M. oculata and its bacterial community fit the definition of holobiont, the versatility of the L. pertusa microbiome suggests that this association is more influenced by the environmental conditions or nutritional status. Our results thus highlight distinct host/microbes association strategies for these two closely related Scleractinians sharing the same habitat, suggesting distinct sensitivity to environmental change.

Published
2016
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37. Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas

Author

Meistertzheim, Anne-Leila, Centre de formation et de recherche sur l'environnement marin (CEFREM), Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and UnivBrestBU, AdminHAL

Subjects
Thermal limit, urogenital system, Reproduction, [SDV]Life Sciences [q-bio], fungi, Temperature, Gonad, Heat Shock Proteins, [SDV] Life Sciences [q-bio], Maturation stage, Crassostrea gigas, ELISA, Sex, Repartition area, Metallothioneins
Abstract

International audience; Heat Shock Protein 70 (HSP70) are generalist stress proteins expressed in response to numerous environmental stresses. However, the baselines of these constitutive proteins are still unknown in many organisms and in particular marine intertidal species such as the Pacific oyster, Crassostrea gigas. I demonstrate that levels of heat shock proteins (HSP) and other stress proteins (metallothioneins, MTs) quantified by ELISA, remained similar in gills, mantle and digestive gland between oysters inhabiting cold and hot sites. In contrast, endogenous HSPs and MTs levels in gonad changed significantly during gametogenesis. In female gonads, the constitutive form of HSP70 and the MTs increased from immature to mature stages (about more than 3-fold) and decreased after spawning. In male gonads, the same expression patterns were observed, whereas protein levels were lower and decreased once fully mature. I hypothesize that the high level of stress proteins in eggs may increase survival of oyster progeny

Published
2013

38. Population genetics of Terapon jarbua

Author

Lavergne, Edouard, Calves, Isabelle, Meistertzheim, Anne-Leila, Charrier, Grégory, Zajonz, Uwe, Laroche, Jean, Lavergne, Edouard, Zajonz, U., Van Damme, K., Lavergne, E., Setzkorn, K. and Jansen van Rensburg, J., LOEWE - Biodiversität und Klima Forschungszentrum (BiK-F), Tropical Marine Ecosystems Group, Senckenberg Forschungsinstitut und Naturmuseum, Ichthyology Section, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de formation et de recherche sur l'environnement marin (CEFREM), Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Marine Ecology, University of Gothenburg (GU), Zajonz, U., Van Damme, K., Lavergne, E., Setzkorn, K. and Jansen van Rensburg, and J.

Subjects
temporarily open closed estuary, [SDE.BE] Environmental Sciences/Biodiversity and Ecology, microsatellite, Yemen, cytochrome c oxidase subunit 1, population genetics, nursery, Socotra, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Terapon jarbua
Abstract

International audience; Few studies were conducted on fish genetic structure in the North-Western Indian Ocean, an area under strong climatic influence, characterized by the alternating monsoon seasons. The particular geography and climate of Soqotra Island is responsible of the presence of wadi and "Khor", which are often separated from the sea by gravelly, sandy berms and roads. Thus, in Soqotra the connectivity between brackish and marine water could be temporally limited. The aim of the present study is to better understand 1) the genetic structure of estuarine fishes on Soqotra and 2) the connectivity between estuarine and marine habitats. Such information could contribute to the first stage for future estuarine conservation plans around Soqotra Island. This study focuses on the grunter Terapon jarbua locally known "Habraham" on Soqotra Island. This species inhabits marine and brackish waters as adults and juveniles respectively, khors playing the role of nurseries. Although this fish is not of particular economical interest on Soqotra, its omnipresence in almost all khors of the region make it a suitable model species to study genetic structure and habitat connectivity. The population structure of T. jarbua is described by analysing sequence variations in a fragment of the mitochondrial gene Cytochrome c Oxidase subunit I (COI) and the genotyping of nine microsatellites in 288 individuals from 10 locations around Soqotra Island and the south coast of Yemen mainland. COI analyses stressed some genetic heterogeneity within this region, with three locations significantly different from the rest. Considering the variability of the microsatellites, this study detected a significant genetic differentiation between estuaries, where geographical distance was not a structuring factor for the populations. Indeed some close genetically related populations were separated from each other by several hundred kilometres while significantly different populations were separated by less than a few kilometres. This spatial structure might be the result of stochastic environmental variables (i.e.: currents, khor opening, mortality) on estuary recruitment, postlarvae settlement and juvenile survival. Thus some nurseries might be randomly dominated by particular subpopulations while others might be colonized by a possible larval pool stemming from different subpopulations. Those hypotheses were later confirmed by micro-chemical studies of the otoliths (earbones). The results demonstrate that it will be difficult to select just a few estuarine sites for conservation as their value in terms of its nursery function will vary in time and space according to the climatic conditions. It is rather recommended that basically all estuaries should enjoy at least a basic level of conservation management.

Published
2012

40. Fossil clams from a serpentinite-hosted sedimented vent field near the active smoker complex Rainbow, MAR, 36 degrees 13 ' N: Insight into the biogeography of vent fauna

Author

Lartaud, Franck, De Rafelis, Marc, Oliver, Graham, Krylova, Elena, Dyment, Jerome, Ildefonse, Benoit, Thibaud, Remy, Gente, Pascal, Hoise, Eva, Meistertzheim, Anne-leila, Fouquet, Yves, Gaill, Francoise, Le Bris, Nadine, Institut des Sciences de la Terre de Paris (iSTeP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO), National Museum of Wales - Cardiff, P.P. Shirshov Institute of Oceanology (SIO), Russian Academy of Sciences [Moscow] (RAS), Laboratoire de Géosciences Marines (LGM), Université Pierre et Marie Curie - Paris 6 (UPMC)-IPG PARIS-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Géosciences Montpellier, Université des Antilles et de la Guyane (UAG)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Naval Academy Research Institute, Domaines Océaniques (LDO), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Observatoire des Sciences de l'Univers-Institut d'écologie et environnement-Centre National de la Recherche Scientifique (CNRS), Laboratoire de géologie de l'ENS (LGENS), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Département des Géosciences - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Biologie et écologie tropicale et méditerranéenne [2007-2010] (BETM), Université de Perpignan Via Domitia (UPVD)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Systématique, adaptation, évolution (SAE), Laboratoire d'Ecogéochimie des environnements benthiques (LECOB), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Environnement Profond (LEP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche de l'Ecole Navale (IRENAV), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Arts et Métiers Sciences et Technologies, HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université de Perpignan Via Domitia (UPVD)-École Pratique des Hautes Études (EPHE), Etudes des Ecosystèmes Profonds (EEP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre National de la Recherche Scientifique (CNRS)-Institut d'écologie et environnement-Observatoire des Sciences de l'Univers-Université de Brest (UBO)-Institut national des sciences de l'Univers (INSU - CNRS), Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Perpignan Via Domitia (UPVD)

Subjects
Mid-Atlantic Ridge, Thyasiridae, carbon and oxygen isotopes, Vesicomyidae, ultramafic-hosted, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography, bivalve shells
Abstract

International audience; Hydrothermal circulation at ultramafic-hosted sites supports a large variety of high-and low-temperature hydrothermal vents and associated ecosystems. The discovery of abundant fossil vesicomyid and thyasirid shell accumulations at the ridge crest, approximately 2.5 km east of the active Rainbow vent field on the Mid-Atlantic Ridge (MAR, 36 degrees 13'N), increased our knowledge regarding the diversity of vent communities at slow spreading ridges. Bivalve molluscs of the family Vesicomyidae were represented by the genus Phreagena. Here we present the first record of this genus in the Atlantic Ocean. This second vesicomyid species known from the MAR, Phreagena sp., was found to be associated with a Thyasira species that is affiliated with T. southwardae (at the Logatchev vent field on the MAR) and with T. vulcolutre (in the Gulf of Cadiz). These two clams have close relationships with seep taxa along the continental margin, and were likely associated with sedimented vent fields. delta O-18 and delta C-13 analyses of the shells suggested that the burrowing bivalve Thyasira could incorporate isotopically light carbon, derived from the oxidation of methane in the sediment, while the signature of Phreagena sp. shells denoted a different carbonate source. C-14 dating of the shells denoted that the hydrothermal activity in the Rainbow area began at least similar to 25.5 kyr BP, which is similar to the model of the hydrothermal vent field distribution that was proposed for the Logatchev hydrothermal site. The results provide new insight regarding the diversity of chemosynthetic fauna on the MAR over geologic time. Ultramafic-hosted, on-axis sedimented vent fields extend the range of habitats for chemosynthetic communities, underlying the need to further explore the geology of these types of environments on slow-spreading ridges and to determine their role in the ecology of deep-sea vent communities.

Published
2010

42. Innate Immune Responses of a Scleractinian Coral to Vibriosis

Author

Vidal-dupiol, Jeremie, Ladriere, Ophelie, Destoumieux Garzon, Delphine, Sautiere, Pierre-eric, Meistertzheim, Anne-leila, Tambutte, Eric, Tambutte, Sylvie, Duval, David, Foure, Laurent, Adjeroud, Mehdi, Mitta, Guillaume, Vidal-dupiol, Jeremie, Ladriere, Ophelie, Destoumieux Garzon, Delphine, Sautiere, Pierre-eric, Meistertzheim, Anne-leila, Tambutte, Eric, Tambutte, Sylvie, Duval, David, Foure, Laurent, Adjeroud, Mehdi, and Mitta, Guillaume

Abstract

Scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are among the most diverse of all ecosystems. Over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographically widespread and a major threat to these ecosystems. Although coral immunity has been the subject of increasing study, the available information remains fragmentary, especially with respect to coral antimicrobial responses. In this study, we characterized damicornin from Pocillopora damicornis, the first scleractinian antimicrobial peptide (AMP) to be reported. We found that its precursor has a segmented organization comprising a signal peptide, an acidic proregion, and the C-terminal AMP. The 40-residue AMP is cationic, C-terminally amidated, and characterized by the presence of six cysteine molecules joined by three intramolecular disulfide bridges. Its cysteine array is common to another AMP and toxins from cnidarians; this suggests a common ancestor, as has been proposed for AMPs and toxins from arthropods. Damicornin was active in vitro against Gram-positive bacteria and the fungus Fusarium oxysporum. Damicornin expression was studied using a combination of immunohistochemistry, reverse phase HPLC, and quantitative RT-PCR. Our data show that damicornin is constitutively transcribed in ectodermal granular cells, where it is stored, and further released in response to nonpathogenic immune challenge. Damicornin gene expression was repressed by the coral pathogen Vibrio coralliilyticus. This is the first evidence of AMP gene repression in a host-Vibrio interaction.

Published
2011
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44. The high resolution melting analysis (HRM) as a molecular tool for monitoring parasites of the wildlife.

Author

HÉRITIER, LAURENT, VERNEAU, OLIVIER, BREUIL, GREGORY, and MEISTERTZHEIM, ANNE-LEILA

Subjects
WILDLIFE monitoring, ENDANGERED species, PET industry, ANIMAL health, INTERNATIONAL trade, GENETIC barcoding, HIGH resolution imaging
Abstract

In an interconnected world, the international pet trade on wild animals is becoming increasingly important. As a consequence, non-native parasite species are introduced, which affect the health of wildlife and contribute to the loss of biodiversity. Because the investigation of parasite diversity within vulnerable host species implies the molecular identification of large samples of parasite eggs, the sequencing of DNA barcodes is time-consuming and costly. Thereby, the objectives of our study were to apply the high resolution melting (HRM) approach for species determination from pools of parasite eggs. Molecular assays were validated on flatworm parasites (polystomes) infecting the Mediterranean pond turtle Mauremys leprosa and the invasive red-eared slider Trachemys scripta elegans in French natural environments. HRM analysis results indicated that double or multiple parasitic infections could be detected from wild animal populations. They also showed that the cycle of parasite eggs production was not regular over time and may depend on several factors, among which the ecological niche and the target species. Thereby, monitoring parasites from wild endangered animals implies periodic parasitological surveys to avoid false negative diagnostics, based solely on eggs production. [ABSTRACT FROM AUTHOR]

Published
2017
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45. Innate Immune Responses of a Scleractinian Coral to Vibriosis

Author

Vidal-Dupiol, Jeremie, primary, Ladrière, Ophélie, additional, Destoumieux-Garzón, Delphine, additional, Sautière, Pierre-Eric, additional, Meistertzheim, Anne-Leila, additional, Tambutté, Eric, additional, Tambutté, Sylvie, additional, Duval, David, additional, Fouré, Laurent, additional, Adjeroud, Mehdi, additional, and Mitta, Guillaume, additional

Published
2011
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50. Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions.

Author

Collin, Hélëne, Meistertzheim, Anne-Leila, David, Elise, Moraga, Dario, and Boutet, Isabelle

Subjects
*PACIFIC oysters, *PESTICIDE pollution, *EFFECT of water pollution on marine organisms, *IRON metabolism, *OXIDATIVE stress
Abstract

Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtractive hybridization technique was used to discover the main physiological function affected by a cocktail of three pesticides (lindane, metolachlor and carbofuran) in the Pacific oyster Crassostrea gigas. Two oyster populations exposed to different pollution levels in the wild were investigated. The pesticide concentrations used to induce stress were close to those found in the wild. In a time course experiment, the expression of three genes implicated in iron metabolism and oxidative stress as well as that of two ubiquitous stress proteins was examined. No clear regulation of gene or protein expression was found, potentially due to a low-dose effect. However, we detected a strong siteand organ-specific response to the pesticides. This study thus (1) provides insight into bivalve responses to pesticide pollution at the level of the transcriptome, which is the first level of response for organisms facing pollution, and (2) raises interesting questions concerning the importance of the sites and organs studied in the toxicogenomic field. [ABSTRACT FROM AUTHOR]

Published
2010
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