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The impact of SARS‐CoV‐2 infection shortly after vaccination on vaccine‐induced immunity is unknown, which is also one of the concerns for some vaccinees during the pandemic. Here, based on a cohort of individuals who encountered BA.5 infection within 8 days after receiving the fourth dose of a bivalent mRNA vaccine, preceded by three doses of inactivated vaccines, we show that booster mRNA vaccination provided 48% protection efficacy against symptomatic infections. At Day 7 postvaccination, the level of neutralizing antibodies (Nabs) against WT and BA.5 strains in the uninfected group trended higher than those in the symptomatic infection group. Moreover, there were greater variations in Nabs levels and a significant decrease in virus‐specific CD4+ T cell response observed in the symptomatic infection group. However, symptomatic BA.5 infection significantly increased Nab levels against XBB.1.9.1 and BA.5 (symptomatic > asymptomatic > uninfected group) at Day 10 and resulted in a more gradual decrease in Nabs against BA.5 compared to the uninfected group at Day 90. Our data suggest that BA.5 infection might hinder the early generation of Nabs and the recall of the CD4+ T cell response but strengthens the Nab and virus‐specific T cell response in the later phase. Our data confirmed that infection can enhance host immunity regardless of the short interval between vaccination and infection and alleviate concerns about infections shortly after vaccination, which provides valuable guidance for developing future vaccine administration strategies. [ABSTRACT FROM AUTHOR]

Background: Chronic obstructive pulmonary disease (COPD) is one of the diseases with high mortality and morbidity with complex pathogenesis. Airway remodeling is an unavoidable pathological characteristic. However, the molecular mechanisms of airway remodeling are incompletely defined. Methods: lncRNAs highly correlated with transforming growth factor beta 1(TGF–β1) expression were chosen, the lncRNA ENST00000440406 (named HSP90AB1 Assoicated LncRNA 1, HSALR1) was chosen for further functional experiments. Dual luciferase and ChIP assay were used to detect the upstream of HSALR1, transcriptome sequencing, Cck–8, Edu, cell proliferation, cell cycle assay, and WB detection of pathway levels confirmed the effect of HSALR1 on fibroblast proliferation and phosphorylation levels of related pathways. Mice was infected with adeno–associated virus (AAV) to express HSALR1 by intratracheal instillation under anesthesia and was exposure to cigarette smoke, then mouse lung function was performed and the pathological sections of lung tissues were analyzed. Results: Herein, lncRNA HSALR1 was identified as highly correlated with the TGF–β1 and mainly expressed in human lung fibroblasts. HSALR1 was induced by Smad3 and promoted fibroblasts proliferation. Mechanistically, it could directly bind to HSP90AB1 protein, and acted as a scaffold to stabilize the binding between Akt and HSP90AB1 to promote Akt phosphorylation. In vivo, mice expressed HSALR1 by AAV was exposure to cigarette smoke (CS) for COPD modeling. We found that lung function was worse and airway remodeling was more pronounced in HSLAR1 mice compare to wild type (WT) mice. Conclusion: Our results suggest that lncRNA HSALR1 binds to HSP90AB1 and Akt complex component, and enhances activity of the TGF–β1 smad3–independent pathway. This finding described here suggest that lncRNA can participate in COPD development, and HSLAR1 is a promising molecular target of COPD therapy. [ABSTRACT FROM AUTHOR]

Under different concentrations of cadmium treatments (Cd2+ concentration: 0, 0.6, 6.0, 12.0 mg/kg, prepared with CdCl2•2.5H2O), pot experiments were conducted to study the application of two calcium-containing substances (Ca2+ concentration: 0, 180, 360 mg/kg, prepared with calcium chloride CaCl2 and lime Ca(OH)2) on the yields of flavonoids and saponins of P. notoginseng. The results showed that the application of calcium chloride and lime could increase the biomass of the main root of P. notoginseng and significantly reduce the cadmium contents. Under 0 and 0.6 mg/kg cadmium treatment, the cadmium content in the main root of P. notoginseng did not exceed the standard value (Cd≤0.5 mg/kg1, GB/T 19086–2008). Under 0.6 mg/kg cadmium treatment, the application of calcium chloride increased the yield of flavonoids in the main root of P. notoginseng. The yield of flavonoids in the main root of P. notoginseng decreased under the treatment of 360 mg/kg Ca2+ lime and 0.6 mg/kg Cd2+. Under the treatments of 6.0 mg/kg and 12.0 mg/kg cadmium stress, calcium chloride and lime reduced the yield of flavonoids in P. notoginseng by 14.2%-35.2%. There was a significant positive logarithmic function correlation between flavonoid yield and ratio of Ca/Cd treatment concentration (P < 0.05). Under the treatment of 0.6 mg/kg Cd2+ with 180 mg/kg Ca2+ (calcium chloride), yields of Rg1, Rb1 and total saponins of P. notoginseng reached the highest and were increased by 114.9%, 323.0% and 172.4%, respectively, compared with no calcium chloride treatment. Under different Cd treatment levels and lime application, the yields of Rg1, Rb1 and total saponins of P. notoginseng decreased and the yield of R1 remained relatively stable. Under a certain concentration of cadmium treatment, appropriate calcium chloride and lime application could increase or maintain the biomass, saponin and flavonoid yields of the main root of P. notoginseng. Yields of R1, Rg1, Rb1 and SPN were influenced due to interaction of Cd2+ and Ca2+. In order to ensure the cadmium content of P. notoginseng less than the standard value and optimal yields of flavonoids and saponins, it could be recommended to apply calcium chloride or lime 1125 kg/hm (180 mg/kg Ca2+) under 0.6 mg/kg Cd2+ treatment. [ABSTRACT FROM AUTHOR]