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Acoustic communication among birds plays an important role in attracting mates and defending territories. For the successful transmission of songs, individuals of different species often avoid singing at the same time to reduce acoustic interference from background noise and overlapping signals from heterospecifics. Such behavioural acoustic niche partitioning may occur especially among closely related species due to their ecological similarities. In this study, we recorded bird sounds in a subtropical forest in China in May–June 2019 and detected seven cuckoo species. Extracting characteristics of the cuckoo calls, we found that only four of the 21 pairs of species overlapped in frequency range, and 19 pairs were classified accurately using a linear discriminant analysis classifier based on their features. The remaining two species pairs could be separated based on temporal or spatial distribution patterns. We also analysed the temporal distribution patterns and overlap time of the calls, finding that the seven species exhibit partitioning in at least one of three acoustic dimensions (site, frequency, activity time). We conclude that the seven sympatric cuckoo species were strongly partitioned in acoustic signal space and minimally masked each other's signals. [ABSTRACT FROM AUTHOR]