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8 results on '"Megumi Yuki"'

1. Nonobese mice with nonalcoholic steatohepatitis fed on a choline‐deficient, l‐amino acid‐defined, high‐fat diet exhibit alterations in signaling pathways

Author

Noriko Suzuki‐Kemuriyama, Akari Abe, Sae Nakane, Kinuko Uno, Shuji Ogawa, Atsushi Watanabe, Ryuhei Sano, Megumi Yuki, Katsuhiro Miyajima, and Dai Nakae

Subjects
nonalcoholic steatohepatitis, ‘nonobese’ NASH subtype, Rho GTPases signaling, Biology (General), QH301-705.5
Abstract

Nonalcoholic steatohepatitis (NASH) is often associated with obesity, but some patients develop NASH without obesity. The physiological processes by which nonobese patients develop NASH and cirrhosis have not yet been determined. Here, we analyzed the effects of dietary methionine content on NASH induced in mice fed on a choline‐deficient, methionine‐lowered, l‐amino acid‐defined high‐fat diet (CDAHFD). CDAHFD with insufficient methionine induced insulin sensitivity and enhanced NASH pathology, but without obesity. In contrast, CDAHFD with sufficient methionine induced steatosis, and unlike CDAHFD with insufficient methionine, also induced obesity and insulin resistance. Gene profile analysis revealed that the disease severity in CDAHFD may partially be due to upregulation of the Rho family GTPases pathway and mitochondrial and nuclear receptor signal dysfunction. The signaling factors/pathways detected in this study may assist in future study of NASH regulation, especially its ‘nonobese’ subtype.

Published
2021
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2. A trans fatty acid substitute enhanced development of liver proliferative lesions induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet

Author

Noriko Suzuki-Kemuriyama, Akari Abe, Kiniko Uno, Shuji Ogawa, Atsushi Watanabe, Ryuhei Sano, Megumi Yuki, Katsuhiro Miyajima, and Dai Nakae

Subjects
Nonalcoholic steatohepatitis, Trans fatty acid substitutes, Choline-deficient, Methionine-lowered, L-amino acid-defined, High-fat diet, Nutritional diseases. Deficiency diseases, RC620-627
Abstract

Abstract Background Nonalcoholic steatohepatitis (NASH) is a form of liver disease characterized by steatosis, necroinflammation, and fibrosis, resulting in cirrhosis and cancer. Efforts have focused on reducing the intake of trans fatty acids (TFAs) because of potential hazards to human health and the increased risk for NASH. However, the health benefits of reducing dietary TFAs have not been fully elucidated. Here, the effects of TFAs vs. a substitute on NASH induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet (CDAA-HF) were investigated. Methods Mice were fed CDAA-HF containing shortening with TFAs (CDAA-HF-T(+)), CDAA-HF containing shortening without TFAs (CDAA-HF-T(−)), or a control chow for 13 or 26 weeks. Results At week 13, NASH was induced in mice by feeding CDAA-HF-T(+) containing TFAs or CDAA-HF-T(−) containing no TFAs, but rather mostly saturated fatty acids (FAs), as evidenced by elevated serum transaminase activity and liver changes, including steatosis, inflammation, and fibrosis. CDAA-HF-T(−) induced a greater extent of hepatocellular apoptosis at week 13. At week 26, proliferative (preneoplastic and non-neoplastic) nodular lesions were more pronounced in mice fed CDAA-HF-T(−) than CDAA-HF-T(+). Conclusions Replacement of dietary TFAs with a substitute promoted the development of proliferation lesions in the liver of a mouse NASH model, at least under the present conditions. Attention should be paid regarding use of TFA substitutes in foods for human consumption, and a balance of FAs is likely more important than the particular types of FAs.

Published
2020
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4. Extract of Siraitia grosvenorii (Luo Han Guo) protects against hepatic fibrosis in mice on a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet without trans fatty acids

Author

Akari Abe, Kinuko Uno, Sae Nakane, Ryuhei Sano, Katsuhiro Miyajima, Atsushi Watanabe, Dai Nakae, Noriko Suzuki-Kemuriyama, Shuji Ogawa, and Megumi Yuki

Subjects
chemistry.chemical_classification, chemistry.chemical_compound, medicine.medical_specialty, Methionine, Endocrinology, chemistry, Internal medicine, medicine, Choline, High fat diet, Siraitia grosvenorii, Hepatic fibrosis, Amino acid
Published
2021

5. Identification of a

Author

Xiaofeng, Cai, Takaaki, Taguchi, Huili, Wang, Megumi, Yuki, Megumi, Tanaka, Kai, Gong, Jinghua, Xu, Yiming, Zhao, Koji, Ichinose, and Aiying, Li

Subjects
Models, Molecular, Bacterial Proteins, Genes, Bacterial, Multigene Family, Glycosyltransferases, Phylogeny, Streptomyces, Biosynthetic Pathways, Naphthoquinones
Published
2021

6. A trans fatty acid substitute enhanced development of liver proliferative lesions induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet

Author

Megumi Yuki, Shuji Ogawa, Akari Abe, Dai Nakae, Ryuhei Sano, Noriko Suzuki-Kemuriyama, Kiniko Uno, Katsuhiro Miyajima, and Atsushi Watanabe

Subjects
Male, 0301 basic medicine, Cirrhosis, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Apoptosis, Choline, Methionine-lowered, Mice, Liver disease, chemistry.chemical_compound, Methionine, 0302 clinical medicine, Endocrinology, Non-alcoholic Fatty Liver Disease, Amino Acids, Insulin-Like Growth Factor I, Phosphorylation, lcsh:RC620-627, chemistry.chemical_classification, Chemistry, Choline-deficient, L-amino acid-defined, NF-kappa B, Organ Size, Trans Fatty Acids, Choline Deficiency, lcsh:Nutritional diseases. Deficiency diseases, High-fat diet, Liver, 030211 gastroenterology & hepatology, Sulfotransferases, medicine.medical_specialty, Clinical chemistry, Diet, High-Fat, Transaminase, 03 medical and health sciences, Internal medicine, medicine, Trans fatty acid substitutes, Animals, Humans, Nonalcoholic steatohepatitis, Inflammation, Gene Expression Profiling, Research, Body Weight, Biochemistry (medical), Correction, Fatty acid, medicine.disease, Animal Feed, Soybean Oil, Fatty Liver, Mice, Inbred C57BL, Disease Models, Animal, 030104 developmental biology, RNA, Steatosis
Abstract

Background Nonalcoholic steatohepatitis (NASH) is a form of liver disease characterized by steatosis, necroinflammation, and fibrosis, resulting in cirrhosis and cancer. Efforts have focused on reducing the intake of trans fatty acids (TFAs) because of potential hazards to human health and the increased risk for NASH. However, the health benefits of reducing dietary TFAs have not been fully elucidated. Here, the effects of TFAs vs. a substitute on NASH induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet (CDAA-HF) were investigated. Methods Mice were fed CDAA-HF containing shortening with TFAs (CDAA-HF-T(+)), CDAA-HF containing shortening without TFAs (CDAA-HF-T(−)), or a control chow for 13 or 26 weeks. Results At week 13, NASH was induced in mice by feeding CDAA-HF-T(+) containing TFAs or CDAA-HF-T(−) containing no TFAs, but rather mostly saturated fatty acids (FAs), as evidenced by elevated serum transaminase activity and liver changes, including steatosis, inflammation, and fibrosis. CDAA-HF-T(−) induced a greater extent of hepatocellular apoptosis at week 13. At week 26, proliferative (preneoplastic and non-neoplastic) nodular lesions were more pronounced in mice fed CDAA-HF-T(−) than CDAA-HF-T(+). Conclusions Replacement of dietary TFAs with a substitute promoted the development of proliferation lesions in the liver of a mouse NASH model, at least under the present conditions. Attention should be paid regarding use of TFA substitutes in foods for human consumption, and a balance of FAs is likely more important than the particular types of FAs.

Published
2020

7. A Trans Fatty Acid Substitute Aggravates Nonalcoholic Steatohepatitis Induced in Mice by Feeding a Choline-Deficient, Methionine-Lowered, L-Amino Acid-Defined, High-Fat Diet

Author

Noriko Suzuki-Kemuriyama, Akari Abe, Kinuko Uno, Shuji Ogawa, Atsushi Watanabe, Ryuhei Sano, Megumi Yuki, Katsuhiro Miyajima, and Dai Nakae

Abstract

Background: Nonalcoholic steatohepatitis (NASH) is a form of liver disease characterized by steatosis, necroinflammation, and fibrosis, resulting in cirrhosis and cancer. Trans fatty acid (TFA) is hazardous for human health and a risk factor of NASH; thus, efforts have focused on reducing its intake. However, the health benefits of reducing dietary TFA are not fully elucidated. We investigated effects of TFA and its substitute on NASH induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet (CDAA-HF). Methods: Mice were fed CDAA-HF containing shortening with TFA (CDAA-HF-T(+)), CDAA-HF containing shortening with a TFA substitute (CDAA-HF-T(−)), or a control chow for 13/26 weeks. Results: CDAA-HF-T(+) contained TFA, whereas CDAA-HF-T(−) contained no TFA and much saturated fatty acids. CDAA-HF-T(+) and CDAA-HF-T(−) induced NASH in mice, evidenced by elevated serum transaminase activity and liver changes, including steatosis, inflammation, and fibrosis. CDAA-HF-T(−) induced more hepatocellular apoptosis and proliferative (preneoplastic and non-neoplastic) nodular lesions than CDAA-HF-T(+). Conclusions: Thus, replacement of dietary TFA with its substitute does not prevent but aggravates nutritionally induced NASH in mice, at least under the present conditions. Attention should be paid regarding future TFA substitute use in humans, and a fatty acid balance is likely more important than the particular types of fatty acids.

Published
2020

8. Leukocyte Emigration in Normal Calves and Calves with Leukocyte Adhesion Deficiency

Author

Megumi Yuki, Hidetoshi Higuchi, H. Noda, Hajime Nagahata, Hiromichi Ohtsuka, Takashi Kurosawa, Asako Masuyama, Hiroshi Sato, and Miyuki Masue

Subjects
Pathology, medicine.medical_specialty, Neutrophils, medicine.drug_class, Leukocyte-Adhesion Deficiency Syndrome, Cattle Diseases, Lumen (anatomy), Complement receptor, Monoclonal antibody, Leukocyte emigration, Cell Movement, In vivo, Cell Adhesion, medicine, Animals, Skin, Leukocyte adhesion deficiency, General Veterinary, medicine.diagnostic_test, business.industry, hemic and immune systems, Pneumonia, Flow Cytometry, medicine.disease, Extravasation, Receptors, Complement, Pulmonary Alveoli, Bronchoalveolar lavage, CD18 Antigens, Luminescent Measurements, Cattle, Female, business, Bronchoalveolar Lavage Fluid
Abstract

The emigration of leukocytes from calves with beta 2 integrin deficiency (BLAD) into bronchoalveolar spaces and scraped tissues was compared to that of normal calves. Polymorphonuclear neutrophils were found in bronchoalveolar lavage fluid from BLAD-affected calves showing chronic pneumonia. The neutrophils were complement receptor type 3 (CR3)-negative when characterized by flow cytometric analysis using anti-CD18 monoclonal antibody. Chemiluminescent response mediated by CR3 in neutrophils isolated from bronchoalveolar lavage fluid from BLAD-calves showed similar findings obtained from CR3-deficient neutrophils. Neutrophils from normal calves migrated into scraped tissue which was prepared in an upper gluteal surface area, whereas few leukocytes from calves with BLAD migrated to the scraped tissue, evaluated by skin window (Rebuck) method. These findings confirmed the extravasation of CR3-deficient leukocytes into bronchoalveolar lumen in BLAD calves, and demonstrated in vivo characteristics of extravasating property of normal and CR3-deficient neutrophils into scraped tissues.

Published
1997

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