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Mother nature furnishes various sources to synthesize nanomaterial’s with different geometry, size, and functionality. In this outline, we aimed to discuss the biological source-mediated fabrication of Ag NPs because of their easy handling, yields, and economical and non-toxicity. The literature reveals that different plant species, fungi, and bacteria can employ biosynthesis, enabling the fabrication of nanoparticles with different features, notably size, geometry, and morphology. The exact mechanisms have not been understood well, even though it is trusted that bio-sourced is responsible for this process. The method of synthesis can be influenced by pH, concentration, time, and biomass. The optimized biosynthesized AgNPs can employ in various domains like sensors, nanomedicine, environmental pollution etc., The main objective of the paper is to elaborate on the biosynthesized AgNPS in electrochemical sensing and its surface modifications. Furthermore, these electroanalytical techniques are to be used for real-time sampling to allow the selective detection of the target analyte.