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Your search keyword '"Medvedko, Alexey V."' showing total 7 results
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7 results on '"Medvedko, Alexey V."'

6. On the development of a new approach to the design of lanthanide-based materials for solution-processed OLEDs

Author

Kozlov, Makarii I., primary, Aslandukov, Andrey N., additional, Vashchenko, Andrey A., additional, Medvedko, Alexey V., additional, Aleksandrov, Alexey E., additional, Grzibovskis, Raitis, additional, Goloveshkin, Alexander S., additional, Lepnev, Leonid S., additional, Tameev, Alexey R., additional, Vembris, Aivars, additional, and Utochnikova, Valentina V., additional

Published
2019
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7. Chemiluminescent and Colorimetric Aptamer-Based Assays of Human α-Thrombin.

Author

Kurseev, Sergei A., Solovjev, Anton M., Neumann, Marina M., Medvedko, Alexey V., and Sakharov, Ivan Yu.

Subjects
COLORIMETRY, CHEMILUMINESCENCE, CHEMILUMINESCENCE assay, THROMBIN, DETECTION limit, FLUORESCEIN
Abstract

A microplate-based sandwich assay for the determination of α-human thrombin (HTb) was developed. Fluorescein-modified 29-mer thrombin binding aptamer (FAM-TBA29) and biotinylated 15-mer thrombin binding aptamer (Bio-TBA15) reacting with different exosites of HTb were used as the biorecognition components in the assay. FAM-TBA29 (capture aptamer) was immobilized using its interaction with anti-fluorescein antibody adsorbed on the microplate surface. As a sensitive signaling system, a combination of Bio-TBA15 and streptavidin-polyHRP conjugate was used. Under the optimized conditions, the detection limit for HTb was 1.4 nM; this value was the same in both the colorimetric and the chemiluminescent assays. The replacement of colorimetry for HRP measurement with chemiluminescence increased the assay sensitivity from 0.06 to 1.7 × 106 nM−1 that clearly demonstrated advantage of the latter approach. [ABSTRACT FROM AUTHOR]

Published
2020
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