5 results on '"Medina, Vanina Araceli"'
Search Results
2. Blood and oxidative stress parameters of oil-spill rehabilitated magallanic penguins
- Author
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Carabajal, Eliana, D'amico, Veronica Laura, Medina, Vanina Araceli, and Bertellotti, Nestor Marcelo
- Subjects
Otras Ciencias de la Salud ,purl.org/becyt/ford/3.3 [https] ,CIENCIAS MÉDICAS Y DE LA SALUD ,Parámetros hematológicos ,Pingüinos de Magallanes ,Ciencias de la Salud ,purl.org/becyt/ford/3 [https] ,Estrés oxidativo ,Oil pollution ,Hematological parameters ,Stress Oxidative ,Contaminación por petróleo ,Magellanic penguins - Abstract
Los pingüinos de Magallanes son entre las aves marinas, la especie más afectada por la contaminación con petróleo en Chubut y Santa Cruz. Una de las consecuencias adversas de la exposición a hidrocarburos y otros contaminantes es el aumento de los niveles celulares de especies reactivas del oxígeno o estrés oxidativo, considerados herramientas útiles como biomarcadores del impacto de la exposición a contaminantes químicos peligrosos. El objetivo de este trabajo fue evaluar parámetros hematológicos y marcadores de estrés oxidativo durante la rehabilitación de tres pingüinos empetrolados provenientes del Área Natural Protegida Punta Tombo, Chubut, Argentina. Se tomaron tres muestras de sangre por individuo: la primera muestra al arribo de los pingüinos al centro de rehabilitación, la segunda una semana después y una última muestra antes de ser liberados. Se obtuvieron la cantidad total de leucocitos, la razón heterófilos/linfocitos, el hematocrito y las concentraciones de glucosa y de proteínas totales. Se analizó la actividad de la enzima catalasa, responsable de la degradación del peróxido de hidrógeno, los niveles de tioles totales no proteicos y el daño a lípidos evaluando las especies reactivas al ácido tiobarbitúrico, como indicadores de estrés oxidativo. El estudio se complementó con la obtención del peso de los pingüinos. En general, los parámetros medidos, aumentaron o se mantuvieron constantes desde la primera toma de muestra hasta la última. Si bien algunas de las variables para cada pingüino se comportaron diferentes durante el tratamiento, en general se observó una tendencia a normalizarse hacia el momento de su liberación. Se concluye que los pingüinos se liberaron en buen estado físico luego de la rehabilitación. Magellanic penguins are among the most affected seabirds by oil contamination in Patagonia. Hydrocarbons and other pollutants cause an increase in the cellular levels of reactive oxygen species that lead oxidative stress and in this way, the evaluation of oxidative stress parameters could be useful tools as biomarkers to evaluate the exposure to hazardous chemical contaminants. The aim of the present work was to evaluate hematological parameters and oxidative stress biomarkers during the rehabilitation of three oil-spill penguins from Punta Tombo Natural Protected Area in Chubut, Argentina. Three blood samples were taken from each individual, the first sample was obtained at arrival of penguin to the rehabilitation center, the second one was the following one week and last sample was taken before animals were freed. Hematocrit, white blood cell count, heterophil/ lymphocyte ratio as a measure of stress, and concentrations of glucose and total proteins were determined. The thiobarbituric acid reactive species, a well-established method for monitoring lipid peroxidation, the activity of catalase enzyme (involved in the catabolism of hydrogen peroxide) and the thiol levels were evaluated as oxidative stress indicators. In general, the measured parameters remained constant or increased their values from the first to the last blood sampling. While some of the variables for each penguin behaved differently during treatment, generally they tended to normalize when penguins were released. We conclude that penguins were released in good physical condition after rehabilitation. Fil: Carabajal, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina Fil: D'amico, Veronica Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bertellotti, Nestor Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
- Published
- 2016
3. Diagnóstico y seguimiento de cáncer de piel no-melanoma utilizando 99mTc-MIBI : estudios en un modelo animal
- Author
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Salgueiro, María Jimena, Tesan, Fiorella Carla, Palmieri, Mónica Alejandra, Durán, Hebe, Medina, Vanina Araceli, Leonardi, Natalia, Goldman, Cinthia Gabriela, Boccio, Jose Ruben, and Zubillaga, Marcela Beatriz
- Subjects
Otras Ciencias de la Salud ,99mTc-MIBI ,purl.org/becyt/ford/3.3 [https] ,CIENCIAS MÉDICAS Y DE LA SALUD ,Ciencias de la Salud ,Cáncer de piel no-melanoma ,purl.org/becyt/ford/3 [https] ,Sencar - Abstract
El objetivo consistió en evaluar la utilidad del 99mTc-MIBI como marcador para diagnóstico y seguimiento de la progresión tumoral del NMSC en un modelo de carcinogénesis completa en ratones. Los animales en estudio fueron inyectados con 99mTc-MIBI a diferentes tiempos y eutanasiados. Se disecaron muestras de tumor y piel sana para evaluar la captación del radiofármaco y realizar el diagnóstico histológico. En animales con 22 semanas de progresión tumoral se observó una diferencia significativa en la captación del 99mTc-MIBI entre piel sana y NMSC. El protocolo que mejor se adapta al uso del 99mTc-MIBI como marcador para el diagnóstico y seguimiento de la progresión tumoral en ratones portadores de NMSC inducidos es la administración i.v de 1 mCi de 99mTc-MIBI con adquisición de datos a los 30 minutos post inyección. Se observó que a medida que los tumores progresan, la captación de 99mTc-MIBI disminuye respecto a la piel normal. The aim of the work was to evaluate the usefulness of 99mTc-MIBI as a tracer for the tumor diagnosis and progression of NMSC in a chemically induced model in mice. After administration of 99mTc-MIBI animals were sacrificed at different times. Samples of tumor and healthy skin were dissected in order to perform histological analysis and to evaluate 99mTc-MIBI uptake. Animals under 22 weeks of tumor evolution showed a statistically difference in 99mTc-MIBI uptake between healthy skin and NMSC. Our results showed that the better protocol for the study of the tumor diagnosis and progression of NMSC in mice is the administration of 1 mCi of 99mTc-MIBI and acquisition of images 30 minutes post injection. Results showed that, as tumor progresses, the uptake of 99mTc-MIBI is significantly lower than healthy skin. Fil: Salgueiro, María Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Tesan, Fiorella Carla. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Palmieri, Mónica Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Durán, Hebe. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leonardi, Natalia. Laboratorios BACON; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Goldman, Cinthia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Boccio, Jose Ruben. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Zubillaga, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
- Published
- 2011
4. Histamine receptors and cancer pharmacology
- Author
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Medina, Vanina Araceli and Rivera, Elena S.
- Subjects
MALIGNANCY ,HISTAMINE H4 RECEPTOR ,CIENCIAS MÉDICAS Y DE LA SALUD ,BREAST CANCER ,HISTAMINE ,CELL PROLIFERATION ,ANTICANCER DRUGS ,MELANOMA ,COLON CANCER ,Medicina Clínica ,CANCER PHARMACOLOGY ,HISTAMINE H4 RECEPTOR LIGANDS ,Oncología - Abstract
Considerable evidence has been collected indicating that histamine can modulate proliferation of different normal and malignant cells. High histamine biosynthesis and content together with histamine receptors have been reported in different human neoplasias including melanoma, colon and breast cancer, as well as in experimental tumours in which histamine has been postulated to behave as an important paracrine and autocrine regulator of proliferation. The discovery of the human histamine H(4) receptor in different tissues has contributed to our understanding of histamine role in numerous physiological and pathological conditions revealing novel functions for histamine and opening new perspectives in histamine pharmacology research. In the present review we aimed to briefly summarize current knowledge on histamine and histamine receptor involvement in cancer before focusing on some recent evidence supporting the novel role of histamine H(4) receptor in cancer progression representing a promising molecular target and avenue for cancer drug development. Fil: Medina, Vanina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Rivera, Elena S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
- Published
- 2010
5. Hepato and Cardiotoxicity of Chemotherapeutic Treatment Evaluated by Means of Small Animal Imaging.
- Author
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Tesan FC, Portillo MG, Martinel-Lamas D, Medina VA, Salgueiro MJ, and Zubillaga MB
- Subjects
- Animals, Antineoplastic Agents administration & dosage, Antineoplastic Agents pharmacology, Cardiotoxicity, Dose-Response Relationship, Drug, Doxorubicin administration & dosage, Doxorubicin pharmacology, Drug Screening Assays, Antitumor, Histamine administration & dosage, Histamine pharmacology, Male, Molecular Structure, Rats, Rats, Sprague-Dawley, Structure-Activity Relationship, Antineoplastic Agents adverse effects, Doxorubicin adverse effects, Heart drug effects, Histamine adverse effects, Liver drug effects
- Abstract
Background: Chemotherapy is one of the most common approaches for cancer treatment. Particularly Doxorubicin has been proven to be effective in the treatment of many soft and solid tumors for locally advanced and metastatic cancer. It is not easy to clinically evaluate the chemotoxic or chemoprotective effect of some drugs, even more when there is a subclinical toxicity., Objective: To determine the usefulness of the hepatobiliary, colloid and cardiac scintigraphies, employing99mTcdisida, 99mTc-phytate and 99mTc-sestamibi respectively, in the evaluation of the hepato and cardiotoxicity of two chemotherapeutic treatments assessed in rats., Method: Two groups were submitted to doxorubicin (DOX) treatment and one was co-administered with histamine (DOX+HIS). Static 99mTc-phytate and 99mTc-sestamibi scintigraphies as well as a dynamic 99mTc-disida study were performed in a small field of view gamma camera at: 0 weeks (control), 1 week and 2 weeks of treatment. Imagenological parameters were calculated: Liver/Bone Marrow ratio (L/BM), Heart/Background ratio (H/B) and time to the maximum (Tmax) for 99mTc-phytate, 99mTc-sestamibi and 99mTc-disida extraction, respectively., Results: Control (L/BM= 98±3; H/B=2.3±0.4; Tmax=8±3), DOX (L/BM: 85±3, 80±3; H/B, 3.5±0.5, 3.3±0.5 and Tmax 6±1, 4±1) for 1 and 2 weeks respectively and DOX+HIS (L/BM: 99±0.3, 98±1; H/B 2.9±0.5, 2.9±0.5 and Tmax, 8±2, 9±2) for 1 and 2 weeks, respectively. Histological analysis showed cardio and hepatotoxicity induced by doxorubicin., Conclusion: Imagenological parameters showed differences among treated and control groups and between both chemotherapy treatments. Thus, these radiopharmaceutical functional approaches were able to reflect heart and liver toxicity produced by doxorubicin., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
- Published
- 2017
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