112 results on '"Medici, Nicola"'
Search Results
2. Humoral response and safety of the third booster dose of BNT162b2 mRNA COVID-19 vaccine in patients with multiple sclerosis treated with ocrelizumab or fingolimod
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Capuano, Rocco, Altieri, Manuela, Conte, Miriana, Bisecco, Alvino, d’Ambrosio, Alessandro, Donnarumma, Giovanna, Grimaldi, Elena, Coppola, Nicola, Medici, Nicola, Galdiero, Massimiliano, Tedeschi, Gioacchino, and Gallo, Antonio
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- 2022
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3. Multiple Sclerosis: From the Application of Oligoclonal Bands to Novel Potential Biomarkers.
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Maglio, Grazia, D'Agostino, Marina, Caronte, Francesco Pio, Pezone, Luciano, Casamassimi, Amelia, Rienzo, Monica, Di Zazzo, Erika, Nappo, Carmela, Medici, Nicola, Molinari, Anna Maria, and Abbondanza, Ciro
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IMMUNOGLOBULIN light chains ,MULTIPLE sclerosis ,CEREBROSPINAL fluid examination ,CEREBROSPINAL fluid ,BIOMARKERS ,CENTRAL nervous system ,NATALIZUMAB - Abstract
Multiple sclerosis is a chronic immune-mediated disorder of the central nervous system with a high heterogeneity among patients. In the clinical setting, one of the main challenges is a proper and early diagnosis for the prediction of disease activity. Current diagnosis is based on the integration of clinical, imaging, and laboratory results, with the latter based on the presence of intrathecal IgG oligoclonal bands in the cerebrospinal fluid whose detection via isoelectric focusing followed by immunoblotting represents the gold standard. Intrathecal synthesis can also be evidenced by the measurement of kappa free light chains in the cerebrospinal fluid, which has reached similar diagnostic accuracy compared to that of oligoclonal bands in the identification of patients with multiple sclerosis; moreover, recent studies have also highlighted its value for early disease activity prediction. This strategy has significant advantages as compared to using oligoclonal band detection, even though some issues remain open. Here, we discuss the current methods applied for cerebrospinal fluid analysis to achieve the most accurate diagnosis and for follow-up and prognosis evaluation. In addition, we describe new promising biomarkers, currently under investigation, that could contribute both to a better diagnosis of multiple sclerosis and to its monitoring of the therapeutic treatment response. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Exosomes: Emerging Modulators of Pancreatic Cancer Drug Resistance
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Di Donato, Marzia, primary, Medici, Nicola, additional, Migliaccio, Antimo, additional, Castoria, Gabriella, additional, and Giovannelli, Pia, additional
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- 2023
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5. The Retinoblastoma-Interacting Zinc-Finger Protein RIZ Is a Downstream Effector of Estrogen Action
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Abbondanza, Ciro, Medici, Nicola, Nigro, Vincenzo, Rossi, Valentina, Gallo, Luigi, Piluso, Giulio, Belsito, Angela, Roscigno, Annarita, Bontempo, Paola, Puca, Annibale A., Molinari, Anna Maria, Moncharmont, Bruno, and Puca, Giovanni A.
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- 2000
6. Interaction of Vault Particles with Estrogen Receptor in the MCF-7 Breast Cancer Cell
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Abbondanza, Ciro, Rossi, Valentina, Roscigno, Annarita, Gallo, Luigi, Belsito, Angela, Piluso, Giulio, Medici, Nicola, Nigro, Vincenzo, Molinari, Anna Maria, Moncharmont, Bruno, and Puca, Giovanni A.
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- 1998
7. Estradiol Receptor of Calf Uterus: Interaction with Heparin-Agarose and Purification
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Molinari, Anna Maria, Medici, Nicola, Moncharmont, Bruno, and Puca, Giovanni Alfredo
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- 1977
8. Differentiation of Myeloid Cell Lines Correlates with a Selective Expression of RIZ Protein
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Gazzerro, Patrizia, Bontempo, Paola, Schiavone, Ettore M., Abbondanza, Ciro, Moncharmont, Bruno, Armetta, Ignazio, Medici, Nicola, Simone, Mariacarla De, Nola, Ernesto, Puca, Giovanni A., and Molinari, Anna Maria
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- 2001
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9. Modulation of RIZ gene expression is associated to estradiol control of MCF-7 breast cancer cell proliferation
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Gazzerro, Patrizia, Abbondanza, Ciro, DʼArcangelo, Andrea, Rossi, Mariangela, Medici, Nicola, Moncharmont, Bruno, and Puca, Giovanni Alfredo
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- 2006
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10. Breast cancer stem cells: the role of sex steroid receptors
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Giovannelli, Pia, primary, Donato, Marzia Di, additional, Galasso, Giovanni, additional, Zazzo, Erika Di, additional, Medici, Nicola, additional, Bilancio, Antonio, additional, Migliaccio, Antimo, additional, and Castoria, Gabriella, additional
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- 2019
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11. Purification of the Estrogen Receptor
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Bresciani, Francesco, primary, Medici, Nicola, additional, Abbondanza, Ciro, additional, Moncharmont, Bruno, additional, and Puca, Giovanni Alfredo, additional
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- 1990
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12. Immunità mediata da IgE e allergie
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Medici Nicola, Peter Paham, C. Abbondanza, A. Balsari, E. Carbone, V. Casolaro, G. Colonna Romano, R. De Palma, G. Ferlazzo, S. Formisano, A. Leonardi, F. Malisan, G. Matarese, N. Medici, F. Nicoletti, C. Pucillo, G. Ruggiero, P. Zanovello, and Medici, Nicola
- Abstract
Il testo si focalizza sul sistema immunitario umano, proponendo una sintesi dei concetti di immunologia relativi al funzionamento del sistema immunitario con un approccio comprensibile, aggiornato e immediato, ed è ricco di esempi clinici volti all'illustrazione dei temi affrontati.
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- 2016
13. Estrogen Induces Looping Between Tumor Suppressor RIZ Gene Promoter 2 with Exon 9a
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De Rosa, C, Di Zazzo, E, Todisco, E, Griffo, E, Spiniello, M, Ombra, M, Moncharmont, B, Perillo, B, MEDICI, Nicola, ABBONDANZA, Ciro, Società Italiana di Patologia e Medicina Traslazionale, American Society for Investigative Pathology, De Rosa, C, Di Zazzo, E, Todisco, E, Griffo, E, Spiniello, M, Ombra, M, Moncharmont, B, Medici, Nicola, Perillo, B, and Abbondanza, Ciro
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Riz2, Riz1, PRDM2, HMT8, epigenetic, DNA-Picked Chromatin, DPC, ESR1, MCF7 - Abstract
NTP1 Background: The dynamic intra- and inter-chromosomal links between specific loci contribute to the creation of cell type-specific gene expression profiles and to gene regulation during differentiation processes. Looping is implicated in bringing together far upstream or downstream regions with the gene promoter and body sites, and in establishing contacts between the 5' and 3' ends of genes, since 3' end-processing factors interact with components of the transcriptional machinery. The tumor suppressor PRDM2/RIZ gene plays a role in controlling cellular processes, such as cell cycle progression and regulation of development. The retinoblastoma proteinineracting zinc-finger gene (RIZ) is estrogen responsive and has two alternative promoters, the more downstream of which, promoter 2, is nearby to an EREsequence and is involved in estrogen receptor transcriptional activation. Methods: With the innovative DNA-Picked Chromatin (DPC) assay after timecourse of 17-ßestradiol (E2) induction of MCF-7 breast cancer cells, we highlight preferential interaction between hormone-responsive RIZ promoter and the polyadenylation sites. Gene expression analysis of induced cell RNA was performed with qRT-PCR assay. Results: Within 60’ of E2 treatment of cells, we have observed increased exon segments, exons 9a and 10 (alternative polyA site), linked to isolated promoter 2 and concomitant decrease of exon10 to RIZ promoter 1. The exon 9a shows a low association to RIZ promoter 1 without E2. qRT-PCR also demonstrated increased exon 9a-containing transcripts. Conclusions: The E2 remodels the chromatin architecture of PRDM2/RIZ gene locus to create a loop for the mRNA transcription with polyA-exon 9a, leading to the production of oncogenic variants.
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- 2012
14. Expression of PRDM1/BLIMP1 and PRDM2/RIZ in the activation of T CD4+ naïve limphocytes
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DE FELICE L, DE ROSA C, PACIFICO M, DI ZAZZO E, ABBONDANZA, Ciro, MONCHARMONT B, MATARESE G. AND PUCA G. A., MEDICI, Nicola, DE FELICE, L, DE ROSA, C, Pacifico, M, DI ZAZZO, E, Medici, Nicola, Abbondanza, Ciro, Moncharmont, B, and Matarese, G. AND PUCA G. A.
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- 2007
15. Molecular profile of sensitization in subjects with short occupational exposure to latex
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Lamberti, Monica, primary, Buonanno, Rosaria, additional, Ritonnaro, Chiara, additional, Giovane, Giancarlo, additional, Crispino, Vincenzo, additional, Feola, Antonia, additional, Medici, Nicola, additional, Sannolo, Nicola, additional, Di Carlo, Angelina, additional, and Di Domenico, Marina, additional
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- 2015
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16. Antibodies against Food Antigens in Patients with Autistic Spectrum Disorders
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de Magistris, Laura, primary, Picardi, Annarita, additional, Siniscalco, Dario, additional, Riccio, Maria Pia, additional, Sapone, Anna, additional, Cariello, Rita, additional, Abbadessa, Salvatore, additional, Medici, Nicola, additional, Lammers, Karen M., additional, Schiraldi, Chiara, additional, Iardino, Patrizia, additional, Marotta, Rosa, additional, Tolone, Carlo, additional, Fasano, Alessio, additional, Pascotto, Antonio, additional, and Bravaccio, Carmela, additional
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- 2013
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17. Identification of a functional estrogen-responsive enhancer element in the promoter 2 of PRDM2 gene in breast cancer cell lines
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Abbondanza, Ciro, primary, De Rosa, Caterina, additional, D'Arcangelo, Andrea, additional, Pacifico, Marianna, additional, Spizuoco, Clorinda, additional, Piluso, Giulio, additional, Di Zazzo, Erika, additional, Gazzerro, Patrizia, additional, Medici, Nicola, additional, Moncharmont, Bruno, additional, and Alfredo Puca, Giovanni, additional
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- 2011
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18. Highlighting chromosome loops in DNA-picked chromatin (DPC)
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Abbondanza, Ciro, primary, De Rosa, Caterina, additional, Ombra, Maria Neve, additional, Aceto, Fabiana, additional, Medici, Nicola, additional, Altucci, Lucia, additional, Moncharmont, Bruno, additional, Puca, Giovanni Alfredo, additional, Porcellini, Antonio, additional, Avvedimento, Enrico Vittorio, additional, and Perillo, Bruno, additional
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- 2011
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19. Comparative gene expression profiling reveals partially overlapping but distinct genomic actions of different antiestrogens in human breast cancer cells
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Scafoglio, Claudio, primary, Ambrosino, Concetta, additional, Cicatiello, Luigi, additional, Altucci, Lucia, additional, Ardovino, Mario, additional, Bontempo, Paola, additional, Medici, Nicola, additional, Molinari, Anna Maria, additional, Nebbioso, Angela, additional, Facchiano, Angelo, additional, Calogero, Raffaele A., additional, Elkon, Ran, additional, Menini, Nadia, additional, Ponzone, Riccardo, additional, Biglia, Nicoletta, additional, Sismondi, Piero, additional, Bortoli, Michele De, additional, and Weisz, Alessandro, additional
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- 2006
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20. The Zn-finger domain of RIZ protein promotes MCF-7 cell proliferation
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Rossi, Mariangela, primary, Abbondanza, Ciro, additional, D'Arcangelo, Andrea, additional, Gazzerro, Patrizia, additional, Medici, Nicola, additional, Moncharmont, Bruno, additional, and Puca, Giovanni Alfredo, additional
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- 2004
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21. Identification of a DNA Binding Protein Cooperating with Estrogen Receptor as RIZ (Retinoblastoma Interacting Zinc Finger Protein)
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Medici, Nicola, primary, Abbondanza, Ciro, additional, Nigro, Vincenzo, additional, Rossi, Valentina, additional, Piluso, Giulio, additional, Belsito, Angela, additional, Gallo, Luigi, additional, Roscigno, Annarita, additional, Bontempo, Paola, additional, Puca, Annibale A., additional, Molinari, Anna Maria, additional, Moncharmont, Bruno, additional, and Puca, Giovanni A., additional
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- 1999
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22. Prostaglandin E2Induction of Binding Activity to CRE and AP-2 Elements in Human T Lymphocytes
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Micali, Ada, primary, Medici, Nicola, additional, Sottile, Antonino, additional, Venza, Mario, additional, Venza, Isabella, additional, Nigro, Vincenzo, additional, Puca, Giovanni Alfredo, additional, and Teti, Diana, additional
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- 1996
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23. Transcriptional control by estradiol receptor
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Puca, Giovanni Alfredo, primary, Medici, Nicola, additional, Abbondanza, Ciro, additional, Armetta, Ignazio, additional, Nigro, Vincenzo, additional, Moncharmont, Bruno, additional, and Molinari, Anna Maria, additional
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- 1995
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24. A novel p53 mutant in human breast cancer revealed by multiple SSCP analysis
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Nigro, Vincenzo, primary, Napolitano, Massimo, additional, Abbondanza, Ciro, additional, Medici, Nicola, additional, Puca, Annibale Alessandro, additional, Schiavulli, Michele, additional, Armetta, Ignazio, additional, Moncharmont, Bruno, additional, Puca, Giovanni Alfredo, additional, and Molinari, Anna Maria, additional
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- 1994
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25. Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor
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Abbondanza, Ciro, primary, de Falco, Antonietta, additional, Nigro, Vincenzo, additional, Medici, Nicola, additional, Armetta, Ignazio, additional, Molinari, Anna Maria, additional, Moncharmont, Bruno, additional, and Puca, Giovanni A., additional
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- 1993
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26. Purified estrogen receptor enhances in vitro transcription
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Nigro, Vincenzo, primary, Molinari, Anna Maria, additional, Armetta, Ignazio, additional, de Falco, Antonietta, additional, Abbondanza, Ciro, additional, Medici, Nicola, additional, and Puca, Giovanni Alfredo, additional
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- 1992
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27. Identification of a functional estrogen-responsive enhancer element in the promoter 2 of PRDM2 gene in breast cancer cell lines.
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Abbondanza, Ciro, De Rosa, Caterina, D'Arcangelo, Andrea, Pacifico, Marianna, Spizuoco, Clorinda, Piluso, Giulio, Di Zazzo, Erika, Gazzerro, Patrizia, Medici, Nicola, Moncharmont, Bruno, and Alfredo Puca, Giovanni
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GENETICS of breast cancer ,ESTROGEN ,GENE enhancers ,PROMOTERS (Genetics) ,CELL lines ,CANCER cells ,RETINOBLASTOMA gene - Abstract
The retinoblastoma protein-interacting zinc-finger ( RIZ) gene, also known as PRDM2, encodes two protein products, RIZ1 and RIZ2, differing for the presence of a 202 aa domain, called PR domain, at the N-terminus of the RIZ1 molecule. While the histone H3 K9 methyltransferase activity of RIZ1 is associated with the negative control of cell proliferation, no information is currently available on either expression regulation of the RIZ2 form or on its biological activity. RIZ proteins act as ER co-activators and promote optimal estrogen response in female reproductive tissues. In estrogen-responsive cells, 17-β estradiol modulates RIZ gene expression producing a shift in the balanced expression of the two forms. Here, we demonstrate that an estrogen-responsive element (ERE) within the RIZ promoter 2 is regulated in a ligand-specific manner by ERα, through both the AF1 and AF2 domains. The pattern of ERα binding, histone H4 acetylation, and histone H3 cyclical methylation of lysine 9 was comparable to other estrogen-regulated promoters. Association of topoisomerase IIβ with the RIZ promoter 2 confirmed the transcriptional activation induced by estrogen. We hypothesize that RIZ2, acting as a negative regulator of RIZ1 function, mediates the proliferative effect of estrogen through regulation of survival and differentiation gene expression. J. Cell. Physiol. 227: 964-975, 2012. © 2011 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
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- 2012
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28. In VitroBinding of the Purified Hormone-Binding Subunit of the Estrogen Receptor to Oligonucleotides Containing Natural or Modified Sequences of an Estrogen-Responsive Element
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Medici, Nicola, primary, Nigro, Vincenzo, additional, Abbondanza, Ciro, additional, Moncharmont, Bruno, additional, Molinari, Anna Maria, additional, and Puca, Giovanni Alfredo, additional
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- 1991
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29. Properties of monomer and dimer forms of purified estrogen receptor
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Medici, Nicola, primary, Abbondanza, Ciro, additional, Nigro, Vincenzo, additional, Armetta, Ignazio, additional, de Falco, Antonietta, additional, Molinari, Anna M., additional, and Puca, Giovanni A., additional
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- 1991
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30. An aprotinin binding site localized in the hormone binding domain of the estrogen receptor from calf uterus
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Nigro, Vincenzo, primary, Medici, Nicola, additional, Abbondanza, Ciro, additional, Minucci, Saverio, additional, Moncharmont, Bruno, additional, Molinari, Anna Maria, additional, and Puca, Giovanni Alfredo, additional
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- 1990
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31. Identification of a Novel Sarcoglycan Gene at 5q33 Encoding a Sarcolemmal 35 kDa Glycoprotein.
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Nigro, Vincenzo, Piluso, Giulio, Belsito, Angela, Politano, Luisa, Puca, Annibale Alessandro, Papparella, Serenella, Rossi, Elena, Viglietto, Giuseppe, Esposito, Maria Grazia, Abbondanza, Ciro, Medici, Nicola, Molinari, Anna Maria, Nigro, Giovanni, and Puca, Giovanni Alfredo
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- 1996
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32. Metal binding sites of the estradiol receptor from calf uterus and their possible role in the regulation of receptor function
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Medici, Nicola, primary, Minucci, Saverio, additional, Nigro, Vincenzo, additional, Abbondanza, Ciro, additional, Armetta, Ignazio, additional, Molinari, Anna Maria, additional, and Puca, Giovanni Alfredo, additional
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- 1989
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33. Aprotinin inhibits the hormone binding of the estrogen receptor from calf uterus
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Nigro, Vincenzo, primary, Medici, Nicola, additional, Abbondanza, Ciro, additional, Minucci, Saverio, additional, Molinari, Anna Maria, additional, and Puca, Giovanni Alfredo, additional
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- 1989
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34. Particulate nature of the unoccupied uterine estrogen receptor
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Molinari, Anna Maria, primary, Medici, Nicola, additional, Armetta, Ignazio, additional, Nigro, Vincenzo, additional, Moncharmont, Bruno, additional, and Puca, Giovanni Alfredo, additional
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- 1985
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35. Exploring the putative role of PRDM1 and PRDM2 transcripts as mediators of T lymphocyte activation
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Erika Di Zazzo, Monica Rienzo, Amelia Casamassimi, Caterina De Rosa, Nicola Medici, Patrizia Gazzerro, Maurizio Bifulco, Ciro Abbondanza, Di Zazzo, Erika, Rienzo, Monica, Casamassimi, Amelia, De Rosa, Caterina, Medici, Nicola, Gazzerro, Patrizia, Bifulco, Maurizio, and Abbondanza, Ciro
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PRDM1/BLIMP1 ,PRDM2/RIZ ,T lymphocyte activation ,T lymphocyte commitment ,Transcription factors ,Transcription regulation ,General Medicine ,Transcription factor ,General Biochemistry, Genetics and Molecular Biology - Abstract
Background T cell activation and programming from their naïve/resting state, characterized by widespread modifications in chromatin accessibility triggering extensive changes in transcriptional programs, is orchestrated by several cytokines and transcription regulators. PRDM1 and PRDM2 encode for proteins with PR/SET and zinc finger domains that control several biological processes, including cell differentiation, through epigenetic regulation of gene expression. Different transcripts leading to main protein isoforms with (PR +) or without (PR-) the PR/SET domain have been described. Although many studies have established the critical PRDM1 role in hematopoietic cell differentiation, maintenance and/or function, the single transcript contribution has not been investigated before. Otherwise, very few evidence is currently available on PRDM2. Here, we aimed to analyze the role of PRDM1 and PRDM2 different transcripts as mediators of T lymphocyte activation. Methods We analyzed the transcription signature of the main variants from PRDM1 (BLIMP1a and BLIMP1b) and PRDM2 (RIZ1 and RIZ2) genes, in human T lymphocytes and Jurkat cells overexpressing PRDM2 cDNAs following activation through different signals. Results T lymphocyte activation induced an early increase of RIZ2 and RIZ1 followed by BLIMP1b increase and finally by BLIMP1a increase. The “first” and the “second” signals shifted the balance towards the PR- forms for both genes. Interestingly, the PI3K signaling pathway modulated the RIZ1/RIZ2 ratio in favor of RIZ1 while the balance versus RIZ2 was promoted by MAPK pathway. Cytokines mediating different Jak/Stat signaling pathways (third signal) early modulated the expression of PRDM1 and PRDM2 and the relationship of their different transcripts confirming the early increase of the PR- transcripts. Different responses of T cell subpopulations were also observed. Jurkat cells showed that the acute transient RIZ2 increase promoted the balancing of PRDM1 forms towards BLIMP1b. The stable forced expression of RIZ1 or RIZ2 induced a significant variation in the expression of key transcription factors involved in T lymphocyte differentiation. The BLIMP1a/b balance shifted in favor of BLIMP1a in RIZ1-overexpressing cells and of BLIMP1b in RIZ2-overexpressing cells. Conclusions This study provides the first characterization of PRDM2 in T-lymphocyte activation/differentiation and novel insights on PRDM1 and PRDM2 transcription regulation during initial activation phases.
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- 2023
36. Humoral response and safety of the third booster dose of BNT162b2 mRNA COVID-19 vaccine in patients with multiple sclerosis treated with ocrelizumab or fingolimod
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Rocco Capuano, Manuela Altieri, Miriana Conte, Alvino Bisecco, Alessandro d’Ambrosio, Giovanna Donnarumma, Elena Grimaldi, Nicola Coppola, Nicola Medici, Massimiliano Galdiero, Gioacchino Tedeschi, Antonio Gallo, Capuano, Rocco, Altieri, Manuela, Conte, Miriana, Bisecco, Alvino, D'Ambrosio, Alessandro, Donnarumma, Giovanna, Grimaldi, Elena, Coppola, Nicola, Medici, Nicola, Galdiero, Massimiliano, Tedeschi, Gioacchino, and Gallo, Antonio
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Booster dose ,Multiple Sclerosis ,COVID-19 Vaccines ,BNT162b2 mRNA vaccine ,Fingolimod Hydrochloride ,SARS-CoV-2 ,COVID-19 Vaccine ,COVID-19 ,Fingolimod ,Antibodies, Viral ,Neurology ,Immunoglobulin G ,Multiple Sclerosi ,Humans ,Neurology (clinical) ,Ocrelizumab ,RNA, Messenger ,BNT162 Vaccine ,Human - Abstract
Background The assessment of the safety and the humoral response to a third booster dose of the BNT162b2 mRNA COVID-19 vaccine is relevant in patients with Multiple Sclerosis (pwMS) treated with Ocrelizumab (OCR) or Fingolimod (FNG). Methods Serum samples were collected from Healthy controls (HCs) and pwMS treated with OCR or FNG at the following time-points: before the first of two vaccine doses (T0); 8 (T1), 16 (T2), 24 (T3) weeks after the first dose; within 8 weeks before (T0b) and after (T1b) the booster dose. IgG antibodies to SARS-CoV-2 trimeric spike protein (Anti-TSP IgG) were quantified and expressed as binding antibody units (BAU)/mL. Results 40 HCs, 28 pwMS on OCR and 19 on FNG were included. At T0b 12 (42.9%) pwMS on OCR and 6 (31.6%) on FNG were still positive while, at T1b 16 (57.14%) pwMS on OCR and 16 (84.2%) on FNG, passed the threshold of positivity. The increase of Anti-TSP IgG levels at T1b was higher for: (i) HCs with respect to OCR (p p = 0.032) groups; (ii) pwMS on FNG compared with pwMS on OCR (p Conclusions The third booster dose of BNT162b2 mRNA vaccine to OCR- and FNG-treated pwMS revives the humoral response, independently of any clinical variable, and manifests a good safety and tolerability profile.
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- 2022
37. Breast cancer stem cells: the role of sex steroid receptors
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Giovanni Galasso, Erika Di Zazzo, Gabriella Castoria, Nicola Medici, Antimo Migliaccio, Pia Giovannelli, Antonio Bilancio, Marzia Di Donato, Giovannelli, Pia, DI DONATO, Marzia, Giovanni, Galasso, Erika Di Zazzo, Medici, Nicola, Bilancio, Antonio, Migliaccio, Antimo, and Castoria, Gabriella
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Therapeutic implications ,0301 basic medicine ,Histology ,Review ,Biology ,03 medical and health sciences ,Breast cancer ,0302 clinical medicine ,Cancer stem cell ,Genetics ,medicine ,Molecular Biology ,Genetics (clinical) ,Estrogen receptor beta ,Sex steroid receptors ,Cancer stem cells ,Cell Biology ,Sex hormone receptor ,medicine.disease ,Androgen receptor ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,Steroids ,Stem cell ,Estrogen receptor alpha ,Hormone - Abstract
Breast cancer (BC) is the most common cancer among women, and current available therapies often have high success rates. Nevertheless, BC might acquire drug resistance and sometimes relapse. Current knowledge about the most aggressive forms of BC points to the role of specific cells with stem properties located within BC, the so-called "BC stem cells" (BCSCs). The role of BCSCs in cancer formation, growth, invasiveness, therapy resistance and tumor recurrence is becoming increasingly clear. The growth and metastatic properties of BCSCs are regulated by different pathways, which are only partially known. Sex steroid receptors (SSRs), which are involved in BC etiology and progression, promote BCSC proliferation, dedifferentiation and migration. However, in the literature, there is incomplete information about their roles. Particularly, there are contrasting conclusions about the expression and role of the classical BC hormonal biomarkers, such as estrogen receptor alpha (ERα), together with scant, albeit promising information concerning ER beta (ERβ) and androgen receptor (AR) properties that control different transduction pathways in BCSCs. In this review, we will discuss the role that SRs expressed in BCSCs play to BC progression and recurrence and how these findings have opened new therapeutic possibilities.
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- 2019
38. Immunità innata: le prime linee di difesa
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ABBONDANZA, Ciro, MEDICI N., Fujita, T, Stappenbeck,T, Tenner, AJ, Locati, Massimo, Mainiero, Fabrizio, Abbondanza, Ciro, Medici, Nicola, MURPHY KENNETH, and Medici, N.
- Abstract
Il testo Immunobiologia di Janeway è stato pensato per gli studenti dei corsi universitari, ma è così completo da essere adeguato anche come testo di riferimento per specializzandi e per gli stessi medici che praticano l’immunologia. Pur addentrandosi nel mondo della microbiologia, è chiaramente focalizzato sullo studio dell’immunologia e dei suoi principali eventi immunopatologici, quali le reazioni autoimmunitarie, le immunodeficienze, i trapianti e l’immunologia dei tumori. Questa nona edizione è stata completamente riorganizzata ed aggiornata e contiene più di cento immagini nuove. Nell’Appendice I, il Toolbox degli immunologi ha subito una totale rivisitazione, con l’aggiunta di nuove tecniche, tra cui il sistema CRISPR/Cas9 e tecniche di spettrometria di massa e di proteomica. Inoltre è stato creato un elenco di domande che serviranno agli studenti per riflettere attentamente e sintetizzare le conoscenze apprese in ogni capitolo e per preparare al meglio gli esami.
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- 2019
39. Immunobiologia di Janeway
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ciro abbondanza, nicola medici, Abbondanza, Ciro, and Medici, Nicola
- Abstract
Il testo Immunobiologia di Janeway è stato pensato per gli studenti dei corsi universitari, ma è così completo da essere adeguato anche come testo di riferimento per specializzandi e per gli stessi medici che praticano l’immunologia. Pur addentrandosi nel mondo della microbiologia, è chiaramente focalizzato sullo studio dell’immunologia e dei suoi principali eventi immunopatologici, quali le reazioni autoimmunitarie, le immunodeficienze, i trapianti e l’immunologia dei tumori. Questa nona edizione è stata completamente riorganizzata ed aggiornata e contiene più di cento immagini nuove. Nell’Appendice I, il Toolbox degli immunologi ha subito una totale rivisitazione, con l’aggiunta di nuove tecniche, tra cui il sistema CRISPR/Cas9 e tecniche di spettrometria di massa e di proteomica. Inoltre è stato creato un elenco di domande che serviranno agli studenti per riflettere attentamente e sintetizzare le conoscenze apprese in ogni capitolo e per preparare al meglio gli esami.
- Published
- 2019
40. Antibodies against Food Antigens in Patients with Autistic Spectrum Disorders
- Author
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Maria Pia Riccio, Dario Siniscalco, Patrizia Iardino, Salvatore Abbadessa, Carmela Bravaccio, Rosa Marotta, Karen M. Lammers, Alessio Fasano, Carlo Tolone, Antonio Pascotto, Annarita Picardi, Chiara Schiraldi, Laura de Magistris, Nicola Medici, Rita Cariello, Anna Sapone, Laura de, Magistri, Annarita, Picardi, Dario, Siniscalco, Maria Pia, Riccio, Anna, Sapone, Rita, Cariello, Salvatore, Abbadessa, Nicola, Medici, Karen M., Lammer, Chiara, Schiraldi, Patrizia, Iardino, Rosa, Marotta, Carlo, Tolone, Alessio, Fasano, Antonio, Pascotto, Bravaccio, Carmela, DE MAGISTRIS, Laura, Picardi, A, Siniscalco, D, Riccio, Mp, Sapone, A, Cariello, R, Abbadessa, Salvatore, Medici, Nicola, Lammers, Km, Schiraldi, Chiara, Iardino, P, Marotta, R, Tolone, Carlo, Fasano, A, Pascotto, Antonio, and Bravaccio, C.
- Subjects
Male ,Article Subject ,lcsh:Medicine ,Immunoglobulin E ,Antibodies ,Gliadin ,Permeability ,General Biochemistry, Genetics and Molecular Biology ,Immune system ,Antigen ,Casein ,medicine ,Humans ,Antigens ,Child ,chemistry.chemical_classification ,Intestinal permeability ,General Immunology and Microbiology ,biology ,lcsh:R ,nutritional and metabolic diseases ,General Medicine ,medicine.disease ,Gluten ,Immunoglobulin A ,Intestines ,Celiac Disease ,Haplotypes ,chemistry ,Child Development Disorders, Pervasive ,Food ,Immunoglobulin G ,Immunology ,biology.protein ,Female ,Antibody ,Research Article - Abstract
Purpose. Immune system of some autistic patients could be abnormally triggered by gluten/casein assumption. The prevalence of antibodies to gliadin and milk proteins in autistic children with paired/impaired intestinal permeability and under dietary regimen either regular or restricted is reported.Methods. 162 ASDs and 44 healthy children were investigated for intestinal permeability, tissue-transglutaminase (tTG), anti-endomysium antibodies (EMA)-IgA, and total mucosal IgA to exclude celiac disease; HLA-DQ2/-DQ8 haplotypes; total systemic antibodies (IgA, IgG, and IgE); specific systemic antibodies:α-gliadin (AGA-IgA and IgG), deamidated–gliadin-peptide (DGP-IgA and IgG), total specific gliadin IgG (all fractions:α,β,γ, andω),β-lactoglobulin IgG,α-lactalbumin IgG, casein IgG; and milk IgE, casein IgE, gluten IgE, -lactoglobulin IgE, andα-lactalbumin IgE.Results. AGA-IgG and DPG-IgG titers resulted to be higher in ASDs compared to controls and are only partially influenced by diet regimen. Casein IgG titers resulted to be more frequently and significantly higher in ASDs than in controls. Intestinal permeability was increased in 25.6% of ASDs compared to 2.3% of healthy children. Systemic antibodies production was not influenced by paired/impaired intestinal permeability.Conclusions. Immune system of a subgroup of ASDs is triggered by gluten and casein; this could be related either to AGA, DPG, and Casein IgG elevated production or to impaired intestinal barrier function.
- Published
- 2013
41. The Zn-finger domain of RIZ protein promotes MCF-7 cell proliferation
- Author
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Nicola Medici, Bruno Moncharmont, Patrizia Gazzerro, M. Rossi, Andrea D'Arcangelo, Ciro Abbondanza, Giovanni Alfredo Puca, Rossi, M., Abbondanza, Ciro, D'Arcangelo, A., Gazzerro, P., Medici, Nicola, Moncharmont, B., and Puca, G. A.
- Subjects
Cancer Research ,Recombinant Fusion Proteins ,Breast Neoplasms ,Biology ,Transfection ,Retinoblastoma-like protein 1 ,Gene product ,Cell proliferation ,Cyclin D1 ,Cell Line, Tumor ,Humans ,Cell Proliferation ,Cell growth ,Nuclear Proteins ,Zinc Fingers ,Histone-Lysine N-Methyltransferase ,Molecular biology ,Fusion protein ,Protein Structure, Tertiary ,DNA-Binding Proteins ,RING finger domain ,Oncology ,Cytoplasm ,Cell culture ,Retinoblastoma-interacting zinc-finger protein ,MCF-7 cell line ,RIZ ,Transcription Factors - Abstract
In order to understand the oncogenic properties of retinoblastoma-interacting zinc-finger (RIZ) gene products, we produced an MCF-7-derived cell line expressing a fusion protein containing the zinc-finger (aa 359–497) domain of RIZ protein (MCF-7/znf). The Zn-finger domain contains three of the eight putative Zn-finger motifs and is located in proximity of the E1A-like domain containing the Rb protein-binding motif. The MCF-7/znf cells showed a higher growth rate than the parental or the control cell lines, both in hormone-deprived conditions or upon estrogen stimulation. Furthermore, they were less sensitive to the growth inhibitory effect of anti-estrogens and showed a higher level of expression of cyclin D1 and A. The expressed Zn-finger domain recombinant product was localized in the nucleus and in the nucleoli and its expression modified the pattern of actin staining in the cytoplasm. In conclusion the presented results indicated that the Zn-finger domain could be endowed with the putative oncogenic activity of RIZ2 gene product.
- Published
- 2004
42. The retinoblastoma-interacting zinc-finger protein RIZ is a downstream effector of estrogen action
- Author
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Ciro Abbondanza, Nicola Medici, Vincenzo Nigro, Valentina Rossi, Luigi Gallo, Giulio Piluso, Angela Belsito, Annarita Roscigno, Paola Bontempo, Annibale A. Puca, Anna Maria Molinari, Bruno Moncharmont, Giovanni A. Puca, Abbondanza, Ciro, Medici, Nicola, Nigro, Vincenzo, Rossi, V, Gallo, L, Piluso, Giulio, Belsito, Angela, Roscigno, A, Bontempo, Paola, Puca, Aa, Molinari, Anna Maria, Moncharmont, B, and Puca, Ga
- Subjects
DNA-Binding Proteins ,Multidisciplinary ,Base Sequence ,Receptors, Estrogen ,Humans ,Nuclear Proteins ,Estrogens ,Zinc Fingers ,Histone-Lysine N-Methyltransferase ,Biological Sciences ,Cell Line ,DNA Primers ,Transcription Factors - Abstract
Co-immunoprecipitation experiments in cell extract from cultured cells or target tissues indicated that estrogen receptor was complexed with the retinoblastoma binding protein RIZ in a ligand-dependent manner. Mapping of interaction sites indicated that in both proteins the same regions and motifs responsible for the interaction of transcriptional co-activator and nuclear receptors were involved. In cultured cells, estradiol induced a redistribution of RIZ protein within the nucleus and in the cytoplasm. A similar effect was produced in vivo , in prepuberal rat endometrium, by administration of a physiological dose of estradiol. Therefore, RIZ protein could be a specific effector of estrogen action downstream of the hormone-receptor interaction, presumably involved in proliferation control.
- Published
- 2000
43. Prostaglandin E2Induction of Binding Activity to CRE and AP-2 Elements in Human T Lymphocytes
- Author
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Giovanni Alfredo Puca, Isabella Venza, Mario Venza, Diana Teti, Vincenzo Nigro, Antonino Sottile, Nicola Medici, Ada Micali, Micali, A, Medici, Nicola, Sottile, A, Venza, M, Venza, I, Nigro, Vincenzo, Puca, Ga, and Teti, D.
- Subjects
IBMX ,T-Lymphocytes ,Immunology ,Prostaglandin E2 ,CRE and AP-2 elements ,T lymphocytes ,Biology ,Transfection ,Dinoprostone ,Jurkat Cells ,chemistry.chemical_compound ,1-Methyl-3-isobutylxanthine ,Okadaic Acid ,Gene expression ,Cyclic AMP ,Humans ,Electrophoretic mobility shift assay ,Enzyme Inhibitors ,Phosphorylation ,Protein Phosphatase Inhibitor ,Cyclic AMP Response Element-Binding Protein ,Promoter Regions, Genetic ,Transcription factor ,Binding Sites ,Forskolin ,Colforsin ,DNA ,Okadaic acid ,Molecular biology ,DNA-Binding Proteins ,Enhancer Elements, Genetic ,Transcription Factor AP-2 ,chemistry ,Transcription Factors - Abstract
Prostaglandins of the E series are immunomodulatory agents which exert inhibitory as well as stimulatory effects on a variety of immune responses. Since it is known that PGE2is able to increase cAMP levels, we investigated whether it can affect gene expression through the activation of the transcription factors which bind enhancer elements in the promoter regions of cAMP-regulated genes. Using electrophoretic mobility shift assay, we demonstrated that a short treatment of human T lymphocytes with PGE2induces specific binding activity to CRE and AP-2, but not AP-1, DNA elements. Since the okadaic acid, a potent protein phosphatase inhibitor, prolongs the induction of the binding activity, phosphorylation events are likely to occur. This activity seems to be due to increased cAMP levels because forskolin and IBMX mimic the effects of PGE2. More interestingly, transfection experiments with CRE–CAT plasmide show that PGE2activates the transcription of a CRE-containing promoter. These data support the positive role for PGE2on some immune functions.
- Published
- 1996
44. Identification of a functional estrogen-responsive enhancer element in the promoter 2 of PRDM2 gene in breast cancer cell lines
- Author
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Giulio Piluso, Patrizia Gazzerro, Caterina De Rosa, Marianna Pacifico, Nicola Medici, Bruno Moncharmont, Ciro Abbondanza, Giovanni Alfredo Puca, Erika Di Zazzo, Clorinda Spizuoco, Andrea D'Arcangelo, Abbondanza, Ciro, De Rosa, C, D'Arcangelo, A, Pacifico, M, Spizuoco, C, Piluso, Giulio, Di Zazzo, E, Gazzerro, P, Medici, Nicola, Moncharmont, B, and Puca, Ga
- Subjects
Physiology ,Cell Survival ,Cellular differentiation ,Clinical Biochemistry ,Molecular Sequence Data ,Breast Neoplasms ,Biology ,Histone H4 ,Histone H3 ,Cell Line, Tumor ,Gene expression ,Chlorocebus aethiops ,Animals ,Humans ,Enhancer ,Promoter Regions, Genetic ,Transcription factor ,Regulation of gene expression ,Base Sequence ,Estradiol ,Nuclear Proteins ,Promoter ,Cell Differentiation ,Cell Biology ,Histone-Lysine N-Methyltransferase ,Molecular biology ,DNA-Binding Proteins ,Gene Expression Regulation, Neoplastic ,Enhancer Elements, Genetic ,COS Cells ,Female ,Transcription Factors - Abstract
""The retinoblastoma protein-interacting zinc-finger (RIZ) gene, also known as PRDM2, encodes two protein products, RIZ1 and RIZ2, differing for the presence of a 202 aa domain, called PR domain, at the N-terminus of the RIZ1 molecule. While the histone H3 K9 methyltransferase activity of RIZ1 is associated with the negative control of cell proliferation, no information is currently available on either expression regulation of the RIZ2 form or on its biological activity. RIZ proteins act as ER co-activators and promote optimal estrogen response in female reproductive tissues. In estrogen-responsive cells, 17-beta estradiol modulates RIZ gene expression producing a shift in the balanced expression of the two forms. Here, we demonstrate that an estrogen-responsive element (ERE) within the RIZ promoter 2 is regulated in a ligand-specific manner by ERa, through both the AF1 and AF2 domains. The pattern of ERa binding, histone H4 acetylation, and histone H3 cyclical methylation of lysine 9 was comparable to other estrogen-regulated promoters. Association of topoisomerase II beta with the RIZ promoter 2 confirmed the transcriptional activation induced by estrogen. We hypothesize that RIZ2, acting as a negative regulator of RIZ1 function, mediates the proliferative effect of estrogen through regulation of survival and differentiation gene expression. J. Cell. Physiol. 227: 964975, 2012. (C) 2011 Wiley Periodicals, Inc.""
- Published
- 2011
45. Highlighting chromosome loops in DNA-picked chromatin (DPC)
- Author
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Ciro Abbondanza, Fabiana Aceto, Nicola Medici, Bruno Perillo, Lucia Altucci, Giovanni Alfredo Puca, Maria Neve Ombra, Enrico V. Avvedimento, Bruno Moncharmont, Caterina De Rosa, Antonio Porcellini, Abbondanza, Ciro, De Rosa, C, Ombra, Mn, Aceto, F, Medici, Nicola, Altucci, Lucia, Moncharmontb, Puca, Ga, Porcellini, A, Avvedimento, Ev, Perillo, B., Abbondanza, C, Medici, N, Altucci, L, Moncharmont, B, Porcellini, Antonio, and Avvedimento, VITTORIO ENRICO
- Subjects
Proteomics ,Cancer Research ,Transcription, Genetic ,Computational biology ,Biology ,Response Elements ,chemistry.chemical_compound ,Transcription (biology) ,Cell Line, Tumor ,Gene expression ,Chromosomes, Human ,Humans ,DNA looping ,Molecular Biology ,Gene ,Genetics ,Histone Demethylases ,Estradiol ,epigenetics ,Chromatin ,Genes, bcl-2 ,nuclear architecture ,chemistry ,Nucleic Acid Conformation ,chromatin ,Female ,transcription ,DNA - Abstract
"Growing evidence supports the concept that dynamic intra-and inter-chromosomal links between specific loci contribute to the creation of cell type-specific gene expression profiles. Therefore, analysis of the establishment of peculiar functional correlations between sites, also distant on linear DNA, that govern the transcriptional process appears to be of fundamental relevance. We propose here an experimental approach showing that 17 beta-estradiol-induced transcription associates to formation of loops between the promoter and termination regions of hormone-responsive genes. This strategy reveals as a tool to be also suitably used, in conjunction with automated techniques, for an extensive analysis of sites shared by multiple genes for induced expression."
- Published
- 2011
46. In VitroBinding of the Purified Hormone-Binding Subunit of the Estrogen Receptor to Oligonucleotides Containing Natural or Modified Sequences of an Estrogen-Responsive Element
- Author
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Vincenzo Nigro, Ciro Abbondanza, Nicola Medici, Anna Maria Molinari, Bruno Moncharmont, Giovanni Alfredo Puca, Medici, Nicola, Nigro, Vincenzo, Abbondanza, Ciro, Moncharmont, B, Molinari, Anna Maria, and Puca, Ga
- Subjects
Protein subunit ,Response element ,Estrogen receptor ,In Vitro Techniques ,Biology ,Endocrinology ,Affinity chromatography ,Animals ,Electrophoretic mobility shift assay ,Molecular Biology ,Dyad symmetry ,Hormone response element ,Chromatography ,Binding Sites ,Base Sequence ,Estradiol ,Oligonucleotide ,General Medicine ,Molecular biology ,Gene Expression Regulation ,Receptors, Estrogen ,Biochemistry ,Cattle ,Electrophoresis, Polyacrylamide Gel ,Female ,hormones, hormone substitutes, and hormone antagonists - Abstract
Estrogen receptor (ER) was purified from calf uterus by immunoaffinity chromatography in the absence of the ligand. The purified ER consists of a mixture of monomer and homodimer forms of 67-kDa hormone-binding subunit (no 90-kDa heat shock protein is present). The purified ER was incubated with a 32P-labeled 61-basepair oligonucleotide containing the sequence of the estrogen response element (ERE) of the Xenopus laevis A2 vitellogenin gene. DNA mobility shift assays showed formation of specific complexes of the ERE containing oligonucleotide with ER, formation which did not require and was not affected by estradiol or antiestrogenic molecules. Both the monomer and the dimer were equally able to interact with the ERE-containing oligonucleotide. Sucrose gradient experiments showed that only the ER monomer is able to interact with an oligonucleotide in which a single mutation destroyed the dyad symmetry of ERE. Multiple symmetric mutations which did not alter the dyad symmetry of ERE nevertheless totally destroyed the ability of the oligonucleotide to form complexes with either the monomeric or dimeric form of ER. These results suggest that ER is able to bind to ERE independently of the presence of estradiol or other proteins and, therefore, that estradiol does not act by modulating the ability of ER to bind to ERE on DNA.
- Published
- 1991
47. Comparative gene expression profiling reveals partially overlapping but distinct genomic actions of different antiestrogens in human breast cancer cells
- Author
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Paola Bontempo, Concetta Ambrosino, Piero Sismondi, Lucia Altucci, Angelo Facchiano, Anna Maria Molinari, Angela Nebbioso, Nicoletta Biglia, Luigi Cicatiello, Nadia Menini, Alessandro Weisz, Riccardo Ponzone, Raffaele A. Calogero, Claudio Scafoglio, Ran Elkon, Michele De Bortoli, Nicola Medici, Mario Ardovino, Scafoglio, C, Ambrosino, C, Cicatiello, L, Altucci, Lucia, Ardovino, M, Bontempo, Paola, Medici, Nicola, Molinari, Anna Maria, Nebbioso, Angela, Facchiano, A, Calogero, Ra, Elkon, R, Menini, N, Ponzone, R, Biglia, N, Sismondi, P, Bortoli, Md, and Weisz, A.
- Subjects
DNA, Complementary ,Transcription, Genetic ,Breast Neoplasms ,Cell Line ,Tumor ,Computational Biology ,DNA ,Complementary ,Estrogen Antagonists ,Estrogens ,Gene Expression Profiling ,Genome ,Human ,Humans ,Mitosis ,Oligonucleotide Array Sequence Analysis ,Promoter Regions ,Genetic ,Transcription ,medicine.drug_class ,estrogen, breast cancer, tamoxifen, raloxifene, ICI 182,780, cDNA microarrays ,Estrogen receptor ,Biology ,Biochemistry ,Cell Line, Tumor ,medicine ,Promoter Regions, Genetic ,Molecular Biology ,Transcription factor ,Estrogen receptor beta ,Genetics ,Genome, Human ,Cell Biology ,Antiestrogen ,Cell biology ,Gene expression profiling ,Selective estrogen receptor modulator ,Estrogen ,Tamoxifen ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Antiestrogens used for breast cancer (BC) treatment differ among each other for the ability to affect estrogen receptor (ER) activity and thereby inhibit hormone-responsive cell functions and viability. We used high-density cDNA microarrays for a comprehensive definition of the gene pathways affected by 17beta-estradiol (E2), ICI 182,780 (ICI), 4OH-tamoxifen (Tamoxifen), and raloxifene (RAL) in ER-positive ZR-75.1 cells, a suitable model to investigate estrogen and antiestrogen actions in hormone-responsive BC. The expression of 601 genes was significantly affected by E2 in these cells; in silico analysis reveals that 86 among them include one or more potential ER binding site within or near the promoter and that the binding site signatures for E2F-1, NF-Y, and NRF-1 transcription factors are significantly enriched in the promoters of genes induced by estrogen treatment, while those for CAC-binding protein and LF-A1 in those repressed by the hormone, pointing to novel transcriptional effectors of secondary responses to estrogen in BC cells. Interestingly, expression of 176 E2-regulated mRNAs was unaffected by any of the antiestrogens tested, despite the fact that under the same conditions the transcriptional and cell cycle stimulatory activities of ER were inhibited. On the other hand, of 373 antiestrogen-responsive genes identified here, 52 were unresponsive to estrogen and 25% responded specifically to only one of the compounds tested, revealing non-overlapping and clearly distinguishable effects of the different antiestrogens in BC cells. As some of these differences reflect specificities of the mechanism of action of the antiestrogens tested, we propose to exploit this gene set for characterization of novel hormonal antagonists and selective estrogen receptor modulators (SERMs) and as a tool for testing new associations of antiestrogens, more effective against BC. (c) 2006 Wiley-Liss, Inc.
- Published
- 2006
48. Modulation of RIZ gene expression is associated to estradiol control of MCF-7 breast cancer cell proliferation
- Author
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Nicola Medici, Ciro Abbondanza, Bruno Moncharmont, Andrea D'Arcangelo, Patrizia Gazzerro, Giovanni Alfredo Puca, M. Rossi, Gazzerro, P., Abbondanza, Ciro, D'Arcangelo, A., Rossi, M., Medici, Nicola, Moncharmont, B., and Puca, G. A.
- Subjects
Chromatin Immunoprecipitation ,Transcription, Genetic ,Genes, myc ,Estrogen receptor ,Repressor ,Gene Expression ,Breast Neoplasms ,Biology ,Retinoblastoma Protein ,protein-interacting zinc-finger protein ,Gene expression ,Tumor Cells, Cultured ,Gene silencing ,Humans ,Gene Silencing ,RNA, Messenger ,Nuclear protein ,RNA, Small Interfering ,Cell Proliferation ,Regulation of gene expression ,MCF-7 cell ,Estradiol ,Reverse Transcriptase Polymerase Chain Reaction ,Nuclear Proteins ,Cell Biology ,Histone-Lysine N-Methyltransferase ,Gene regulation ,Molecular biology ,Chromatin ,DNA-Binding Proteins ,Receptors, Estrogen ,Female ,Chromatin immunoprecipitation ,RIZ ,Transcription Factors - Abstract
The retinoblastoma protein-interacting zinc-finger (RIZ) gene, a member of the nuclear protein methyltransferase superfamily, is characterized by the presence of the N-terminal PR domain. The RIZ gene encodes for two proteins, RIZ1 and RIZ2. While RIZ1 contains the PR (PRDI-BF1 and RIZ homologous) domain, RIZ2 lacks it. RIZ gene expression is altered in a variety of human cancers and RIZ1 is now considered to be a candidate tumor suppressor. Estradiol treatment of MCF-7 cells produced a selective decrease of RIZ1 transcript and an increase of total RIZ mRNA. Experiments of chromatin immunoprecipitation indicated that RIZ2 protein expression was controlled by estrogen receptor and RIZ1 had a direct repressor function on c-myc gene expression. To investigate the role of RIZ gene products as regulators of the proliferation/differentiation transition, we analyzed the effects of forced suppression of RIZ1 induced in MCF-7 cells by siRNA of the PR domain-containing form. Silencing of RIZ1 expression stimulated cell proliferation, similar to the effect of estradiol on these cells, associated with a transient increase of c-myc expression.
- Published
- 2005
49. Differentiation of myeloid cell lines correlates with a selective expression of RIZ protein
- Author
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Bruno Moncharmont, Paola Bontempo, Ignazio Armetta, M De Simone, Em Schiavone, Am Molinari, Nicola Medici, E. Nola, Patrizia Gazzerro, Ciro Abbondanza, Ga Puca, Gazzerro, P, Bontempo, Paola, Schiavone, Em, Abbondanza, Ciro, Moncharmont, B, Armetta, I, Medici, Nicola, DE SIMONE, M, Nola, Ernesto, Puca, Ga, and Molinari, Anna Maria
- Subjects
Immunoblotting ,Retinoic acid ,Antineoplastic Agents ,Tretinoin ,Retinoic acid receptor beta ,Biology ,Benzoates ,Retinoblastoma Protein ,Adenoviridae ,Retinoic acid-inducible orphan G protein-coupled receptor ,Retinoids ,chemistry.chemical_compound ,Genetics ,Humans ,Myeloid Cells ,Molecular Biology ,Cells, Cultured ,Genetics (clinical) ,Nuclear Proteins ,Cell Differentiation ,Zinc Fingers ,Histone-Lysine N-Methyltransferase ,Retinoic acid receptor gamma ,Retinoid X receptor gamma ,Immunohistochemistry ,Molecular biology ,Adenosine Monophosphate ,DNA-Binding Proteins ,Retinoic acid receptor ,P19 cell ,chemistry ,Retinoic acid receptor alpha ,Molecular Medicine ,Research Article ,Transcription Factors - Abstract
BACKGROUND: The retinoblastoma-interacting zinc-finger gene RIZ is expressed in two forms (RIZ1 and RIZ2) that differ for the presence near the N-terminus of RIZ1 of a conserved domain, defined PR (PRDI-BF1-RIZ homology), homologous to a similar domain present in other proteins recognized as tumor suppressor gene products. The RIZ1 form is usually absent or expressed at low levels in tumor cells, whereas RIZ2 is frequently expressed. We investigated a possible involvement of RIZ1 in differentiation control using a myeloid cell maturation model that is easily modulated by retinoids and other agents. MATERIALS AND METHODS: HL60 or NB4 cell lines or patients' leukemic promyelocytes were treated with all- trans -retinoic acid or other agents to induce differentiation. RIZ gene expression was determined with reverse transcriptase polymerase chain reaction (RT-PCR) and RNase protection assay. Immunocytochemistry was performed to assess variation of the intracellular distribution of RIZ protein on all- trans-retinoic acid treatment. Forced expression of RIZ1 protein was obtained with a recombinant adenovirus containing RIZ1 cDNA. RESULTS: Treatment with retinoic acid induced a selective expression of RIZ1 in HL60 cell line. Retinoic acid effect was maximal at 7 days and correlated to the granulocytic differentiation of cells. A similar effect was obtained in retinoic acid-sensitive NB4 cell line or in patients' leukemic promyelocytes, but not in the retinoic acid-resistant cell line NB4.007/6 or in the U937 cell line. Selective expression of RIZ1 was also induced by 12-O-tetradecanoyl-phorbol-13-acetate in the U937 and HL60 cell lines and by 1,25-dihydroxyvitamin D(3) only in HL60 cells. In HL60 cells, RIZ1 was also induced by activation of a retinoid alpha receptor-independent maturation pathway based on retinoid X receptor agonist and protein kinase A synergism. In addition, retinoic acid produced a redistribution of the antigen within the nucleus in these cells. Forced expression of RIZ1 protein induced growth arrest and death of HL60 cells. CONCLUSIONS: The correlation between the selective expression of RIZ1 induced by retinoic acid, 12-O-tetradecanoyl-phorbol-13-acetate, or 1,25-dihydroxyvitamin D(3) and differentiation suggested that RIZ protein was involved in myeloid cell differentiation induced by these agents.
- Published
- 2001
50. Prostaglandin E2 signalling pathway in human T lymphocytes from healthy and conjunctiva basal cell carcinoma-bearing subjects
- Author
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G Ceci, G Piraino, Nicola Medici, L Giordano, Isabella Venza, Diana Teti, Venza, I, Giordano, L, Piraino, G, Medici, Nicola, Ceci, G, and Teti, D.
- Subjects
Adult ,Transcriptional Activation ,Immunoprecipitation ,conjunctival neoplasms ,Recombinant Fusion Proteins ,T-Lymphocytes ,Immunology ,Immunoblotting ,T lymphocytes ,Biology ,CREB ,environment and public health ,Jurkat cells ,Retinoblastoma Protein ,Dinoprostone ,Jurkat Cells ,cyclic AMP responsive element-binding protein ,Genes, Reporter ,Immunology and Allergy ,Humans ,Phosphorylation ,Protein kinase A ,Cyclic AMP Response Element-Binding Protein ,Activating Transcription Factor 2 ,Kinase ,prostaglandin ,Cell Biology ,Molecular biology ,Precipitin Tests ,Carcinoma, Basal Cell ,biology.protein ,Signal transduction ,CREB1 ,Signal Transduction ,Transcription Factors - Abstract
Prostaglandin E-induced signal transduction pathways in human T cells from healthy and uveal melanoma-bearing subjects were studied. Transfection experiments showed that PGE2 was able to phosphorylate and activate the fusion trans-activator of the cAMP responsive element-binding protein (CREB). Phosphorylation was at least partially mediated by protein kinase A, as evidenced by the effects of specific kinase inhibitors. Western blotting experiments, which were performed to identify the CREB/ATF2 family members involved in the response to PGE2, revealed a modulation of proteins CREB1, CREB2 and ATF2 and phosphorylation of the 43 kDa form of CREB. Experiments of immunoprecipitation with CREB-binding protein (CBP) demonstrated that, after PGE2 treatment, all of the CREB/ATF isoforms studied, as well as the phosphorylated form of CREB (p-CREB), interacted with CBP. In basal conditions, T cells from patients with conjunctiva basal cell carcinoma showed the presence of p-CREB, which coimmunoprecipitated with CBP. CREB phosphorylation did not modify after PGE2 treatment whereas the p-CREB fraction bound to CBP increased in a delayed manner compared to normal subjects.
- Published
- 2001
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