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2. IMPORTÂNCIA DO ACOMPANHAMENTO CONTÍNUO DE PACIENTES FALCIFORMES COM A ATENÇÃO MULTIDISCIPLINAR. RELATO DE CASO DE IRMÃS ATENDIDAS NO HEMONORTE-RN
- Author
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Silva, LKF, primary, Gadelha, FP, additional, Meireles, EESB, additional, Bahia, IAF, additional, Medeiros, TC, additional, Fernandes, TAAM, additional, Spinelli, MDSD, additional, Moreir-Júnior, VF, additional, Moura, ZMA, additional, and Cavalcant-Júnior, GB, additional
- Published
- 2023
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3. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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Klionsky, DJ, Abdel-Aziz, AK, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, MH, Abudu, YP, Acevedo-Arozena, A, Adamopoulos, IE, Adeli, K, Adolph, TE, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, BB, Agnello, M, Agostinis, P, Agrewala, JN, Agrotis, A, Aguilar, PV, Ahmad, ST, Ahmed, ZM, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, HA, Al-Abd, AM, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, MA, Alberti, S, Alcocer-Gómez, E, Alessandri, C, Ali, M, Alim Al-Bari, MA, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, GD, Altan-Bonnet, N, Altieri, DC, Álvarez, ÉMC, Alves, S, Alves da Costa, C, Alzaharna, MM, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, AO, Ammanathan, V, An, Z, Andersen, SU, Andrabi, SA, Andrade-Silva, M, Andres, AM, Angelini, S, Ann, D, Anozie, UC, Ansari, MY, Antas, P, Antebi, A, Antón, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araújo, WL, Araya, J, Arden, C, Arévalo, M-A, Arguelles, S, Arias, E, Arikkath, J, Arimoto, H, Ariosa, AR, Armstrong-James, D, Arnauné-Pelloquin, L, Aroca, A, Arroyo, DS, Arsov, I, Artero, R, Asaro, DML, Aschner, M, Ashrafizadeh, M, Ashur-Fabian, O, Atanasov, AG, Au, AK, Auberger, P, Auner, HW, Aurelian, L, Autelli, R, Avagliano, L, Ávalos, Y, Aveic, S, Aveleira, CA, Avin-Wittenberg, T, Aydin, Y, Ayton, S, Ayyadevara, S, Azzopardi, M, Baba, M, Backer, JM, Backues, SK, Bae, D-H, Bae, O-N, Bae, SH, Baehrecke, EH, Baek, A, Baek, S-H, Baek, SH, Bagetta, G, Bagniewska-Zadworna, A, Bai, H, Bai, J, Bai, X, Bai, Y, Bairagi, N, Baksi, S, Balbi, T, Baldari, CT, Balduini, W, Ballabio, A, Ballester, M, Balazadeh, S, Balzan, R, Bandopadhyay, R, Banerjee, S, Bánréti, Á, Bao, Y, Baptista, MS, Baracca, A, Barbati, C, Bargiela, A, Barilà, D, Barlow, PG, Barmada, SJ, Barreiro, E, Barreto, GE, Bartek, J, Bartel, B, Bartolome, A, Barve, GR, Basagoudanavar, SH, Bassham, DC, Bast, RC, Basu, A, Batoko, H, Batten, I, Baulieu, EE, Baumgarner, BL, Bayry, J, Beale, R, Beau, I, Beaumatin, F, Bechara, LRG, Beck, GR, Beers, MF, Begun, J, Behrends, C, Behrens, GMN, Bei, R, Bejarano, E, Bel, S, Behl, C, Belaid, A, Belgareh-Touzé, N, Bellarosa, C, Belleudi, F, Belló Pérez, M, Bello-Morales, R, Beltran, JSDO, Beltran, S, Benbrook, DM, Bendorius, M, Benitez, BA, Benito-Cuesta, I, Bensalem, J, Berchtold, MW, Berezowska, S, Bergamaschi, D, Bergami, M, Bergmann, A, Berliocchi, L, Berlioz-Torrent, C, Bernard, A, Berthoux, L, Besirli, CG, Besteiro, S, Betin, VM, Beyaert, R, Bezbradica, JS, Bhaskar, K, Bhatia-Kissova, I, Bhattacharya, R, Bhattacharya, S, Bhattacharyya, S, Bhuiyan, MS, Bhutia, SK, Bi, L, Bi, X, Biden, TJ, Bijian, K, Billes, VA, Binart, N, Bincoletto, C, Birgisdottir, AB, Bjorkoy, G, Blanco, G, Blas-Garcia, A, Blasiak, J, Blomgran, R, Blomgren, K, Blum, JS, Boada-Romero, E, Boban, M, Boesze-Battaglia, K, Boeuf, P, Boland, B, Bomont, P, Bonaldo, P, Bonam, SR, Bonfili, L, Bonifacino, JS, Boone, BA, Bootman, MD, 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ED, Chan, HYE, Chan, HH, Chan, H, Chan, MTV, Chan, YS, Chandra, PK, Chang, C-P, Chang, C, Chang, H-C, Chang, K, Chao, J, Chapman, T, Charlet-Berguerand, N, Chatterjee, S, Chaube, SK, Chaudhary, A, Chauhan, S, Chaum, E, Checler, F, Cheetham, ME, Chen, C-S, Chen, G-C, Chen, J-F, Chen, LL, Chen, L, Chen, M, Chen, M-K, Chen, N, Chen, Q, Chen, R-H, Chen, S, Chen, W, Chen, X-M, Chen, X-W, Chen, X, Chen, Y, Chen, Y-G, Chen, Y-J, Chen, Y-Q, Chen, ZS, Chen, Z, Chen, Z-H, Chen, ZJ, Cheng, H, Cheng, J, Cheng, S-Y, Cheng, W, Cheng, X, Cheng, X-T, Cheng, Y, Cheng, Z, Cheong, H, Cheong, JK, Chernyak, BV, Cherry, S, Cheung, CFR, Cheung, CHA, Cheung, K-H, Chevet, E, Chi, RJ, Chiang, AKS, Chiaradonna, F, Chiarelli, R, Chiariello, M, Chica, N, Chiocca, S, Chiong, M, Chiou, S-H, Chiramel, AI, Chiurchiù, V, Cho, D-H, Choe, S-K, Choi, AMK, Choi, ME, Choudhury, KR, Chow, NS, Chu, CT, Chua, JP, Chua, JJE, Chung, H, Chung, KP, Chung, S, Chung, S-H, Chung, Y-L, Cianfanelli, V, Ciechomska, IA, Cifuentes, M, 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Davis, T, Dayalan Naidu, S, De Amicis, F, De Bosscher, K, De Felice, F, De Franceschi, L, De Leonibus, C, de Mattos Barbosa, MG, De Meyer, GRY, De Milito, A, De Nunzio, C, De Palma, C, De Santi, M, De Virgilio, C, De Zio, D, Debnath, J, DeBosch, BJ, Decuypere, J-P, Deehan, MA, Deflorian, G, DeGregori, J, Dehay, B, Del Rio, G, Delaney, JR, Delbridge, LMD, Delorme-Axford, E, Delpino, MV, Demarchi, F, Dembitz, V, Demers, ND, Deng, H, Deng, Z, Dengjel, J, Dent, P, Denton, D, DePamphilis, ML, Der, CJ, Deretic, V, Descoteaux, A, Devis, L, Devkota, S, Devuyst, O, Dewson, G, Dharmasivam, M, Dhiman, R, di Bernardo, D, Di Cristina, M, Di Domenico, F, Di Fazio, P, Di Fonzo, A, Di Guardo, G, Di Guglielmo, GM, Di Leo, L, Di Malta, C, Di Nardo, A, Di Rienzo, M, Di Sano, F, Diallinas, G, Diao, J, Diaz-Araya, G, Díaz-Laviada, I, Dickinson, JM, Diederich, M, Dieudé, M, Dikic, I, Ding, S, Ding, W-X, Dini, L, Dinić, J, Dinic, M, Dinkova-Kostova, AT, Dionne, MS, Distler, JHW, Diwan, A, Dixon, IMC, Djavaheri-Mergny, M, Dobrinski, I, Dobrovinskaya, O, Dobrowolski, R, Dobson, RCJ, Đokić, J, Dokmeci Emre, S, Donadelli, M, Dong, B, Dong, X, Dong, Z, Dorn Ii, GW, Dotsch, V, Dou, H, Dou, J, Dowaidar, M, Dridi, S, Drucker, L, Du, A, Du, C, Du, G, Du, H-N, Du, L-L, du Toit, A, Duan, S-B, Duan, X, Duarte, SP, Dubrovska, A, Dunlop, EA, Dupont, N, Durán, RV, Dwarakanath, BS, Dyshlovoy, SA, Ebrahimi-Fakhari, D, Eckhart, L, Edelstein, CL, Efferth, T, Eftekharpour, E, Eichinger, L, Eid, N, Eisenberg, T, Eissa, NT, Eissa, S, Ejarque, M, El Andaloussi, A, El-Hage, N, El-Naggar, S, Eleuteri, AM, El-Shafey, ES, Elgendy, M, Eliopoulos, AG, Elizalde, MM, Elks, PM, Elsasser, H-P, Elsherbiny, ES, Emerling, BM, Emre, NCT, Eng, CH, Engedal, N, Engelbrecht, A-M, Engelsen, AST, Enserink, JM, Escalante, R, Esclatine, A, Escobar-Henriques, M, Eskelinen, E-L, Espert, L, Eusebio, M-O, Fabrias, G, Fabrizi, C, Facchiano, A, Facchiano, F, Fadeel, B, Fader, C, Faesen, AC, Fairlie, WD, Falcó, A, Falkenburger, BH, 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E-K, Tan, Y-Q, Tanaka, M, Tang, D, Tang, J, Tang, T-S, Tanida, I, Tao, Z, Taouis, M, Tatenhorst, L, Tavernarakis, N, Taylor, A, Taylor, GA, Taylor, JM, Tchetina, E, Tee, AR, Tegeder, I, Teis, D, Teixeira, N, Teixeira-Clerc, F, Tekirdag, KA, Tencomnao, T, Tenreiro, S, Tepikin, AV, Testillano, PS, Tettamanti, G, Tharaux, P-L, Thedieck, K, Thekkinghat, AA, Thellung, S, Thinwa, JW, Thirumalaikumar, VP, Thomas, SM, Thomes, PG, Thorburn, A, Thukral, L, Thum, T, Thumm, M, Tian, L, Tichy, A, Till, A, Timmerman, V, Titorenko, VI, Todi, SV, Todorova, K, Toivonen, JM, Tomaipitinca, L, Tomar, D, Tomas-Zapico, C, Tomić, S, Tong, BC-K, Tong, C, Tong, X, Tooze, SA, Torgersen, ML, Torii, S, Torres-López, L, Torriglia, A, Towers, CG, Towns, R, Toyokuni, S, Trajkovic, V, Tramontano, D, Tran, Q-G, Travassos, LH, Trelford, CB, Tremel, S, Trougakos, IP, Tsao, BP, Tschan, MP, Tse, H-F, Tse, TF, Tsugawa, H, Tsvetkov, AS, Tumbarello, DA, Tumtas, Y, Tuñón, MJ, Turcotte, S, Turk, B, Turk, V, Turner, BJ, 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C, Viret, C, Viscomi, MT, Visnjic, D, Vitale, I, Vocadlo, DJ, Voitsekhovskaja, OV, Volonté, C, Volta, M, Vomero, M, Von Haefen, C, Vooijs, MA, Voos, W, Vucicevic, L, Wade-Martins, R, Waguri, S, Waite, KA, Wakatsuki, S, Walker, DW, Walker, MJ, Walker, SA, Walter, J, Wandosell, FG, Wang, B, Wang, C-Y, Wang, C, Wang, D, Wang, F, Wang, G, Wang, H, Wang, H-G, Wang, J, Wang, K, Wang, L, Wang, MH, Wang, M, Wang, N, Wang, P, Wang, QJ, Wang, Q, Wang, QK, Wang, QA, Wang, W-T, Wang, W, Wang, X, Wang, Y, Wang, Y-Y, Wang, Z, Warnes, G, Warnsmann, V, Watada, H, Watanabe, E, Watchon, M, Wawrzyńska, A, Weaver, TE, Wegrzyn, G, Wehman, AM, Wei, H, Wei, L, Wei, T, Wei, Y, Weiergräber, OH, Weihl, CC, Weindl, G, Weiskirchen, R, Wells, A, Wen, RH, Wen, X, Werner, A, Weykopf, B, Wheatley, SP, Whitton, JL, Whitworth, AJ, Wiktorska, K, Wildenberg, ME, Wileman, T, Wilkinson, S, Willbold, D, Williams, B, Williams, RSB, Williams, RL, Williamson, PR, Wilson, RA, Winner, B, Winsor, NJ, Witkin, SS, Wodrich, H, Woehlbier, U, Wollert, T, Wong, E, Wong, JH, Wong, RW, Wong, VKW, Wong, WW-L, Wu, A-G, Wu, C, Wu, J, Wu, KK, Wu, M, Wu, S-Y, Wu, S, Wu, WKK, Wu, X, Wu, Y-W, Wu, Y, Xavier, RJ, Xia, H, Xia, L, Xia, Z, Xiang, G, Xiang, J, Xiang, M, Xiang, W, Xiao, B, Xiao, G, Xiao, H, Xiao, H-T, Xiao, J, Xiao, L, Xiao, S, Xiao, Y, Xie, B, Xie, C-M, Xie, M, Xie, Y, Xie, Z, Xilouri, M, Xu, C, Xu, E, Xu, H, Xu, J, Xu, L, Xu, WW, Xu, X, Xue, Y, Yakhine-Diop, SMS, Yamaguchi, M, Yamaguchi, O, Yamamoto, A, Yamashina, S, Yan, S, Yan, S-J, Yan, Z, Yanagi, Y, Yang, C, Yang, D-S, Yang, H, Yang, H-T, Yang, J-M, Yang, J, Yang, L, Yang, M, Yang, P-M, Yang, Q, Yang, S, Yang, S-F, Yang, W, Yang, WY, Yang, X, Yang, Y, Yao, H, Yao, S, Yao, X, Yao, Y-G, Yao, Y-M, Yasui, T, Yazdankhah, M, Yen, PM, Yi, C, Yin, X-M, Yin, Y, Yin, Z, Ying, M, Ying, Z, Yip, CK, Yiu, SPT, Yoo, YH, Yoshida, K, Yoshii, SR, Yoshimori, T, Yousefi, B, Yu, B, Yu, H, Yu, J, Yu, L, Yu, M-L, Yu, S-W, Yu, VC, Yu, WH, Yu, Z, Yuan, J, Yuan, L-Q, Yuan, S, Yuan, S-SF, Yuan, Y, Yuan, Z, Yue, J, Yue, Z, Yun, J, Yung, RL, Zacks, DN, Zaffagnini, G, Zambelli, VO, Zanella, I, Zang, QS, Zanivan, S, Zappavigna, S, Zaragoza, P, Zarbalis, KS, Zarebkohan, A, Zarrouk, A, Zeitlin, SO, Zeng, J, Zeng, J-D, Žerovnik, E, Zhan, L, Zhang, B, Zhang, DD, Zhang, H, Zhang, H-L, Zhang, J, Zhang, J-P, Zhang, KYB, Zhang, LW, Zhang, L, Zhang, M, Zhang, P, Zhang, S, Zhang, W, Zhang, X, Zhang, X-W, Zhang, XD, Zhang, Y, Zhang, Y-D, Zhang, Y-Y, Zhang, Z, Zhao, H, Zhao, L, Zhao, S, Zhao, T, Zhao, X-F, Zhao, Y, Zheng, G, Zheng, K, Zheng, L, Zheng, S, Zheng, X-L, Zheng, Y, Zheng, Z-G, Zhivotovsky, B, Zhong, Q, Zhou, A, Zhou, B, Zhou, C, Zhou, G, Zhou, H, Zhou, J, Zhou, K, Zhou, R, Zhou, X-J, Zhou, Y, Zhou, Z-Y, Zhou, Z, Zhu, B, Zhu, C, Zhu, G-Q, Zhu, H, Zhu, W-G, Zhu, Y, Zhuang, H, Zhuang, X, Zientara-Rytter, K, Zimmermann, CM, Ziviani, E, Zoladek, T, Zong, W-X, Zorov, DB, Zorzano, A, Zou, W, Zou, Z, Zuryn, S, Zwerschke, W, Brand-Saberi, B, Dong, XC, Kenchappa, CS, Lin, Y, Oshima, S, Rong, Y, Sluimer, JC, Stallings, CL, and Tong, C-K
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
- Published
- 2021
4. Repair bond strength of bulk fill composites after different adhesion protocols
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de Medeiros, TC, primary, de Lima, MR, additional, Bessa, SCF, additional, de Araujo, DFG, additional, and Galvao, MR, additional
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- 2019
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5. Twelve months of emicizumab prophylaxis in a severe hemophilia A man with inhibitor who failed immune tolerance induction: effectiveness, economic, and safety outcomes.
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Camelo RM, de Medeiros TC, de Albuquerque DGB, and Álvares-Teodoro J
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- 2023
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6. Microbial Metabolites Annotation by Mass Spectrometry-Based Metabolomics.
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Gomes PWP, de Tralia Medeiros TC, Maimone NM, Leão TF, de Moraes LAB, and Bauermeister A
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- Humans, Metabolomics methods, Mass Spectrometry methods, Computational Biology, Microbiota, Biological Products metabolism
- Abstract
Since the discovery of penicillin, microbial metabolites have been extensively investigated for drug discovery purposes. In the last decades, microbial derived compounds have gained increasing attention in different fields from pharmacognosy to industry and agriculture. Microbial metabolites in microbiomes present specific functions and can be associated with the maintenance of the natural ecosystems. These metabolites may exhibit a broad range of biological activities of great interest to human purposes. Samples from either microbial isolated cultures or microbiomes consist of complex mixtures of metabolites and their analysis are not a simple process. Mass spectrometry-based metabolomics encompass a set of analytical methods that have brought several improvements to the microbial natural products field. This analytical tool allows the comprehensively detection of metabolites, and therefore, the access of the chemical profile from those biological samples. These analyses generate thousands of mass spectra which is challenging to analyse. In this context, bioinformatic metabolomics tools have been successfully employed to accelerate and facilitate the investigation of specialized microbial metabolites. Herein, we describe metabolomics tools used to provide chemical information for the metabolites, and furthermore, we discuss how they can improve investigation of microbial cultures and interactions., (© 2023. The Author(s), under exclusive license to Springer Nature Switzerland AG.)
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- 2023
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7. Root canal morphology of 1316 premolars from Brazilian individuals: an in vivo analysis using cone-beam computed tomography.
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Lemos MC, Coutinho TM, Perez AR, Medeiros TC, Marceliano-Alves MF, and Alves FR
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- Male, Female, Humans, Bicuspid diagnostic imaging, Bicuspid anatomy & histology, Brazil, Cone-Beam Computed Tomography methods, Dental Pulp Cavity diagnostic imaging, Tooth Root
- Abstract
The aim of this study was to investigate the internal root canal anatomy of maxillary and mandibular premolars in a Brazilian subpopulation, in order to establish the prevalence of the different configurations proposed by Vertucci. Three hundred and ninety-eight cone-beam computed tomography scans were collected from a private imaging clinic database in Rio de Janeiro, including 217 maxillary and 226 mandibular scans. A total 1316 premolars (594 maxillary and 722 mandibular) were evaluated using an image viewer, and classified according to Vertucci. Two calibrated examiners determined the frequency of each morphological Type. A third examiner reviewed discordant cases. The Kappa test was applied to verify inter-rater agreement, and Fisher's Exact Test to verify gender-related differences. The most frequent root canal configurations of maxillary first and second premolars were Type IV (73.86%) and Type I (47.18%), respectively. Type I was the most prevalent in mandibular first and second premolars (80.59% and 95.86%, respectively). Only Types I and VIII presented a statistically significant difference between sexes. Type I was more frequent in females and Type VIII in males. A highly significant frequency of Type I was found in both mandibular first and second premolars, whereas the most frequent maxillary premolar root canal configuration was Type IV for first premolars and Type I for second premolars., Competing Interests: The authors declare no potential conflicts of interest regarding the research, authorship, and/or publication of this article., (Sociedad Argentina de Investigación Odontológica.)
- Published
- 2022
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8. Mitochondria shed their outer membrane in response to infection-induced stress.
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Li X, Straub J, Medeiros TC, Mehra C, den Brave F, Peker E, Atanassov I, Stillger K, Michaelis JB, Burbridge E, Adrain C, Münch C, Riemer J, Becker T, and Pernas LF
- Subjects
- Animals, Cell Line, GTP Phosphohydrolases metabolism, Humans, Intracellular Membranes physiology, Intracellular Membranes ultrastructure, Mice, Mitochondrial Membrane Transport Proteins metabolism, Mitochondrial Membranes ultrastructure, Mitochondrial Proteins metabolism, Protein Binding, Stress, Physiological, Toxoplasma growth & development, Toxoplasma ultrastructure, Toxoplasmosis parasitology, Vacuoles physiology, Vacuoles ultrastructure, Mitochondrial Membranes physiology, Mitochondrial Precursor Protein Import Complex Proteins metabolism, Protozoan Proteins metabolism, Toxoplasma physiology
- Abstract
The outer mitochondrial membrane (OMM) is essential for cellular homeostasis. Yet little is known of the mechanisms that remodel it during natural stresses. We found that large “SPOTs” (structures positive for OMM) emerge during Toxoplasma gondii infection in mammalian cells. SPOTs mediated the depletion of the OMM proteins mitofusin 1 and 2, which restrict parasite growth. The formation of SPOTs depended on the parasite effector TgMAF1 and the host mitochondrial import receptor TOM70, which is required for optimal parasite proliferation. TOM70 enabled TgMAF1 to interact with the host OMM translocase SAM50. The ablation of SAM50 or the overexpression of an OMM-targeted protein promoted OMM remodeling independently of infection. Thus, Toxoplasma hijacks the formation of SPOTs, a cellular response to OMM stress, to promote its growth.
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- 2022
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9. Shaping ability of reciprocating and rotary systems in oval-shaped root canals: a microcomputed tomography study.
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Medeiros TC, Lima CO, Barbosa AFA, Augusto CM, Bruno AMV, Lopes RT, Amoroso-Silva PA, and Marceliano-Alves MF
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- Equipment Design, Root Canal Preparation, X-Ray Microtomography, Dental Pulp Cavity diagnostic imaging, Molar
- Abstract
This study compared the shaping ability of single-file reciprocating (WaveOne Gold) and multifile rotary (Mtwo) systems on mandibular oval-shaped canine root canals, using microcomputed tomography (micro-CT). Thirty mandibular canines were scanned by micro-CT and assigned to one of two groups (n=15) according to the system used for root canal preparation: WaveOne Gold or Mtwo. After preparation, the teeth were rescanned, and the percentage of untouched canal area, apical transportation and centering ability were analyzed. The data was evaluated using Kruskal and Mann- Whitney tests (p<0.05). No difference was found in percentage of unprepared canal area between groups in the entire root canal or the apical third, or in centering ability (p>0.05). WaveOne gold had less canal transportation than MTwo at the 5 mm section (p<0.05). WOG and Mtwo systems presented similar shaping ability and centering ability in oval-shaped canals. However, WOG presented less transportation than Mtwo at 5 mm from the apex., Competing Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article, (Sociedad Argentina de Investigación Odontológica.)
- Published
- 2021
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10. Contact and competition between mitochondria and microbes.
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Medeiros TC, Mehra C, and Pernas L
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- Homeostasis, Host-Pathogen Interactions, Mitochondria
- Abstract
Invading microbes occupy the host cytosol and take up nutrients on which host organelles are also dependent. Thus, host organelles are poised to interact with intracellular microbes. Despite the essential role of host mitochondria in cellular metabolic homeostasis and in mediating cellular responses to microbial infection, we know little of how these organelles interact with intracellular pathogens, and how such interactions affect disease pathogenesis. Here, we give an overview of the different classes of physical and metabolic interactions reported to occur between mitochondria and eukaryotic pathogens. Investigating the underlying molecular mechanisms and functions of such interactions will reveal novel aspects of infection biology., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
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- 2021
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11. Botryosphaeran, a (1 → 3)(1 → 6)-β-D-glucan, reduces tumor development and cachexia syndrome in obese male rats by increasing insulin sensitivity and FOXO3a activity.
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Geraldelli D, Ribeiro MC, Medeiros TC, Comiran PK, Martins KO, Oliveira MF, Oliveira GA, Dekker RFH, Barbosa-Dekker AM, Alegranci P, and Queiroz EAIF
- Subjects
- Animals, Ascomycota chemistry, Cachexia etiology, Cachexia genetics, Cachexia pathology, Cell Proliferation drug effects, Disease Models, Animal, Forkhead Box Protein O3 genetics, Gene Expression Regulation, Neoplastic drug effects, Glucans chemistry, Glucose metabolism, Humans, Insulin genetics, Insulin Resistance genetics, Male, Neoplasms etiology, Neoplasms genetics, Neoplasms pathology, Obesity complications, Obesity genetics, Obesity pathology, Rats, Cachexia drug therapy, Glucans pharmacology, Neoplasms drug therapy, Obesity drug therapy
- Abstract
Obesity is an important risk factor in tumor development. Botryosphaeran, a (1 → 3)(1 → 6)-β-D-glucan, produced by the fungus Botryosphaeria rhodina (MAMB-05), is a high molecular mass, water-soluble exopolysaccharide. It consists of a main chain of (1 → 3)-linked β-d-glucose units, with a degree of branching of ~22% at carbon-6 with glucose and gentiobiose residues linked through β-(1 → 6)-bonds, and presents a triple helix conformation. Botryosphaeran presents anticlastogenic, antiproliferative, pro-apoptotic and anti-obesogenic activities. This study evaluated the effects of botryosphaeran on tumor development in obesity and analyzed its mechanism of action. Obesity was induced in male Wistar rats by a high-fat/high-sugar diet. After 9 weeks, rats were divided into two groups: Obese Tumor (OT) and Obese Tumor Botryosphaeran (OTB), and inoculated with 1 × 10
7 Walker-256 tumor cells, and treatment with botryosphaeran (30 mg/kg b.w./day via gavage for 15 days) commenced. On the 11th week, biological parameters, tumor development, metabolic profile, erythrogram and protein expression were evaluated. Botryosphaeran significantly reduced tumor growth, body-weight loss and cachexia. Furthermore, botryosphaeran decreased mesenteric fat and insulin resistance, corrected macrocytic anemia, and increased Forkhead transcription factor-3a (FOXO3a) activity. Our study demonstrated the potential role of botryosphaeran in the management of cancer in tumor-bearing obese rats by increasing insulin sensitivity and FOXO3a activity., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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12. Tumor development in rats and cancer cachexia are reduced by treatment with botryosphaeran by increasing apoptosis and improving the metabolic profile.
- Author
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Geraldelli D, Ribeiro MC, Medeiros TC, Comiran PK, Martins KO, Oliveira MF, Oliveira GA, Dekker RFH, Barbosa-Dekker AM, Alegranci P, and Queiroz EAIF
- Subjects
- Animals, Cachexia etiology, Carcinoma 256, Walker pathology, Glucans pharmacology, Glucose metabolism, Lipid Metabolism drug effects, Male, Rats, Rats, Wistar, Xenograft Model Antitumor Assays, Apoptosis drug effects, Cachexia drug therapy, Carcinoma 256, Walker drug therapy, Glucans administration & dosage
- Abstract
Aims: Cancer is a multifactorial disease characterized by an uncontrolled growth of cells that can lead to cachexia-anorexia syndrome. Botryosphaeran, a fungal (1 → 3)(1 → 6)-β-D-glucan produced by Botryosphaeria rhodina MAMB-05, has presented antimutagenic, antiproliferative, pro-apoptotic, hypoglycemic and hypocholesterolemic effects. This study evaluated the effects of botryosphaeran (30 mg/kg b.w./day) on tumor development and cachexia syndrome in Walker-256 tumor-bearing rats, and also the metabolic and hematological profiles of these animals., Materials and Methods: Male Wistar rats were divided into 3 groups: control (C), control tumor (CT) and control tumor botryosphaeran (CTB). On the first day, 1 × 10
7 Walker-256 tumor cells were inoculated subcutaneously into the right flank of the CT and CTB rats, and concomitantly treatment with botryosphaeran (30 mg/kg b.w./day) started. After the 15th day of treatment, biological parameters, tumor development, cachexia, glucose and lipid profiles, hemogram and protein expression were analyzed., Key Findings: Botryosphaeran significantly reduced tumor development (p = 0.0024) and cancer cachexia, modulated the levels of glucose, triglycerides and HDL-cholesterol, and corrected macrocytic anemia. Botryosphaeran also increased significantly the bax expression in the tumor tissue (p = 0.038) demonstrating that this (1 → 3)(1 → 6)-β-D-glucan is increasing the apoptosis of tumor cells. p53, p27, bcl-2, caspase-3 and Forkhead transcription factor 3a (FOXO3a) protein expression were similar among the groups., Significance: This study demonstrated that botryosphaeran was effective in decreasing tumor development and cachexia by direct and indirect mechanisms increasing apoptosis and improving the metabolic and hematological profiles., Competing Interests: Declaration of competing interest The authors declare no conflicts of interest., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2020
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13. North-eastern Brazilian recommendations for the nurse professional role on the pharmacokinetic-assisted prophylaxis individualization for haemophilia A.
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de Morais Costa NC, Costa ÍM, Guimarães TMR, de Souza HP, Casaretto ES, de Medeiros TC, Barbosa SM, Matos AIEL, Oliveira EG, and Camelo RM
- Subjects
- Brazil, Factor VIII pharmacokinetics, Female, Humans, Nurses, Professional Role, Factor VIII therapeutic use, Hemophilia A drug therapy
- Published
- 2020
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14. Quality of life and work improvements according to community health agents.
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da Silva Pinheiro L, Souza de Medeiros TC, Valença CN, Ferreira Dantas DK, and Paulo Dos Santos MA
- Abstract
Background: For acting at the level of the community and mediating between it and health services, the role of community health agents (CHA) is essential. For this reason, this occupational group needs satisfactory quality of life and working conditions to ensure their well-being and improve the quality of their work. On these grounds, the present study is justified as a means to reflect on the work process of this occupational group and enable suggestions for improvements., Objective: To discuss strategies to improve CHA's quality of life and work., Methods: Exploratory study with qualitative analysis conducted with CHA in Currais Novos, Rio Grande do Norte, Brazil., Results: Strategies suggested to improve quality of life and work focused on mental and physical aspects and the essential resources needed for work., Conclusion: Discussions and sound grounds are needed for initiatives to improve the living and working conditions of CHA and thus achieve a positive impact on this population of workers, and consequently also on their daily actions.
- Published
- 2020
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15. Autophagy determines mtDNA copy number dynamics during starvation.
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Medeiros TC and Graef M
- Subjects
- DNA Copy Number Variations, DNA-Directed DNA Polymerase, Mitochondria, Autophagy, DNA, Mitochondrial
- Abstract
Derived from bacterial ancestors, mitochondria have maintained their own albeit strongly reduced genome, mitochondrial DNA (mtDNA), which encodes for a small and highly specialized set of genes. MtDNA exists in tens to thousands of copies packaged in numerous nucleoprotein complexes, termed nucleoids, distributed throughout the dynamic mitochondrial network. Our understanding of the mechanisms of how cells regulate the copy number of mitochondrial genomes has been limited. Here, we summarize and discuss our recent findings that Mip1/POLG (mitochondrial DNA polymerase gamma) critically controls mtDNA copy number by operating in 2 opposing modes, synthesis and, unexpectedly, degradation of mtDNA, when yeast cells face nutrient starvation. The balance of the 2 modes of Mip1/POLG and thus mtDNA copy number dynamics depends on the integrity of macroautophagy/autophagy, which sustains continuous synthesis and maintenance of mtDNA. In autophagy-deficient cells, a combination of nucleotide insufficiency and elevated mitochondrial ROS production impairs mtDNA synthesis and drives mtDNA degradation by the 3'-5'-exonuclease activity of Mip1/POLG resulting in mitochondrial genome depletion and irreversible respiratory deficiency. Abbrivations: mtDNA: mitochondrial DNA; mtDCN: mitochondrial DNA copy number.
- Published
- 2019
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16. Autophagy balances mtDNA synthesis and degradation by DNA polymerase POLG during starvation.
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Medeiros TC, Thomas RL, Ghillebert R, and Graef M
- Subjects
- DNA Polymerase gamma genetics, Gene Dosage, Models, Biological, Mutation genetics, Nucleotides metabolism, Reactive Oxygen Species metabolism, Saccharomyces cerevisiae, Autophagy, DNA Polymerase gamma metabolism, DNA, Mitochondrial biosynthesis
- Abstract
Mitochondria contain tens to thousands of copies of their own genome (mitochondrial DNA [mtDNA]), creating genetic redundancy capable of buffering mutations in mitochondrial genes essential for cellular function. However, the mechanisms regulating mtDNA copy number have been elusive. Here we found that DNA synthesis and degradation by mtDNA polymerase γ (POLG) dynamically controlled mtDNA copy number in starving yeast cells dependent on metabolic homeostasis provided by autophagy. Specifically, the continuous mtDNA synthesis by POLG in starving wild-type cells was inhibited by nucleotide insufficiency and elevated mitochondria-derived reactive oxygen species in the presence of autophagy dysfunction. Moreover, after prolonged starvation, 3'-5' exonuclease-dependent mtDNA degradation by POLG adjusted the initially increasing mtDNA copy number in wild-type cells, but caused quantitative mtDNA instability and irreversible respiratory dysfunction in autophagy-deficient cells as a result of nucleotide limitations. In summary, our study reveals that mitochondria rely on the homeostatic functions of autophagy to balance synthetic and degradative modes of POLG, which control copy number dynamics and stability of the mitochondrial genome., (© 2018 Medeiros et al.)
- Published
- 2018
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17. Biosorption of metal ions using a low cost modified adsorbent (Mauritia flexuosa): experimental design and mathematical modeling.
- Author
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Melo Dde Q, Vidal CB, Medeiros TC, Raulino GS, Dervanoski A, Pinheiro Mdo C, and Nascimento RF
- Subjects
- Adsorption, Kinetics, Metals analysis, Metals chemistry, Research Design, Water Pollutants, Chemical analysis, Water Pollutants, Chemical chemistry, Water Purification, Arecaceae chemistry, Computer Simulation, Metals metabolism, Water Pollutants, Chemical metabolism
- Abstract
Buriti fibers were subjected to an alkaline pre-treatment and tested as an adsorbent to investigate the adsorption of copper, cadmium, lead and nickel in mono- and multi-element aqueous solutions, the results showed an increase in the adsorption capacity compared to the unmodified Buriti fiber. The effects of pH, adsorbent mass, agitation rate and initial metal ions concentration on the efficiency of the adsorption process were studied using a fractional 2(4-1) factorial design, and the results showed that all four parameters influenced metal adsorption differently. Fourier transform infrared spectrometry and X-ray fluorescence analysis were used to identify the groups that participated in the adsorption process and suggest its mechanisms and they indicated the probable mechanisms involved in the adsorption process are mainly ion exchange. Kinetic and thermodynamic equilibrium parameters were determined. The adsorption kinetics were adjusted to the homogeneous diffusion model. The adsorption equilibrium was reached in 30 min for Cu(2+) and Pb(2+), 20 min for Ni(2+) and instantaneously for Cd(2+). The results showed a significant difference was found in the competitiveness for the adsorption sites. A mathematical model was used to simulate the breakthrough curves in multi-element column adsorption considering the influences of external mass transfer and intraparticle diffusion resistance.
- Published
- 2016
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18. Ceramide signaling targets the PP2A-like protein phosphatase Sit4p to impair vacuolar function, vesicular trafficking and autophagy in Isc1p deficient cells.
- Author
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Teixeira V, Medeiros TC, Vilaça R, Ferreira J, Moradas-Ferreira P, and Costa V
- Subjects
- Signal Transduction, Transport Vesicles physiology, Unfolded Protein Response, Autophagy physiology, Ceramides physiology, Protein Phosphatase 2 physiology, Saccharomyces cerevisiae Proteins physiology, Type C Phospholipases physiology, Vacuoles physiology
- Abstract
The vacuoles play important roles in cellular homeostasis and their functions include the digestion of cytoplasmic material and organelles derived from autophagy. Conserved nutrient signaling pathways regulate vacuolar function and autophagy, ensuring normal cell and organismal development and aging. Recent evidence implicates sphingolipids in the modulation of these processes, but the impact of ceramide signaling on vacuolar dynamics and autophagy remains largely unknown. Here, we show that yeast cells lacking Isc1p, an orthologue of mammalian neutral sphingomyelinase type 2, exhibit vacuolar fragmentation and dysfunctions, namely decreased Pep4p-mediated proteolysis and V-ATPase activity, which impairs vacuolar acidification. Moreover, these phenotypes are suppressed by downregulation of the ceramide-activated protein phosphatase Sit4p. The isc1Δ cells also exhibit defective Cvt and vesicular trafficking in a Sit4p-dependent manner, ultimately contributing to a reduced autophagic flux. Importantly, these phenotypes are also suppressed by downregulation of the nutrient signaling kinase TORC1, which is known to inhibit Sit4p and autophagy, or Sch9p. These results support a model in which Sit4p functions downstream of Isc1p in a TORC1-independent, ceramide-dependent signaling branch that impairs vacuolar function and vesicular trafficking, leading to autophagic defects in yeast., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2016
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19. Antibacterial activity of commercially available plant-derived essential oils against oral pathogenic bacteria.
- Author
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Bardají DK, Reis EB, Medeiros TC, Lucarini R, Crotti AE, and Martins CH
- Subjects
- Actinomyces drug effects, Fusobacterium nucleatum drug effects, Microbial Sensitivity Tests, Mouth microbiology, Prevotella nigrescens drug effects, Streptococcus mutans drug effects, Anti-Bacterial Agents pharmacology, Cinnamomum zeylanicum chemistry, Oils, Volatile pharmacology, Plant Oils pharmacology
- Abstract
This work investigated the antibacterial activity of 15 commercially available plant-derived essential oils (EOs) against a panel of oral pathogens. The broth microdilution method afforded the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the assayed EOs. The EO obtained from Cinnamomum zeylanicum (Lauraceae) (CZ-EO) displayed moderate activity against Fusobacterium nucleatum (MIC and MBC = 125 μg/mL), Actinomyces naeslundii (MIC and MBC = 125 μg/mL), Prevotella nigrescens (MIC and MBC = 125 μg/mL) and Streptococcus mutans (MIC = 200 μg/mL; MBC = 400 μg/mL). (Z)-isosafrole (85.3%) was the main chemical component of this oil. We did not detect cinnamaldehyde, previously described as the major constituent of CZ-EO, in specimens collected in other countries.
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- 2016
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20. Ceramide signalling impinges on Sit4p and Hog1p to promote mitochondrial fission and mitophagy in Isc1p-deficient cells.
- Author
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Teixeira V, Medeiros TC, Vilaça R, Pereira AT, Chaves SR, Côrte-Real M, Moradas-Ferreira P, and Costa V
- Subjects
- Humans, Mitogen-Activated Protein Kinases genetics, Protein Phosphatase 2 genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Type C Phospholipases metabolism, Ceramides metabolism, Mitochondrial Dynamics physiology, Mitogen-Activated Protein Kinases metabolism, Mitophagy physiology, Protein Phosphatase 2 metabolism, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism, Signal Transduction physiology, Type C Phospholipases deficiency
- Abstract
Mitochondria function as the powerhouses of the cell for energy conversion through the oxidative phosphorylation process. Accumulation of dysfunctional mitochondria promotes a bioenergetic crisis and cell death by apoptosis. Yeast cells lacking Isc1p, an orthologue of mammalian neutral sphingomyelinase type 2, exhibit mitochondrial dysfunction and shortened lifespan associated with the accumulation of specific ceramide species and activation of the PP2A-like protein phosphatase Sit4p and of the Hog1p kinase. Here, we show that isc1Δ cells display hyperactivation of mitophagy that is suppressed by downregulating Sit4p, Hog1p or the TORC1-Sch9p pathway. Notably, isc1Δ cells also have high levels of Dnm1p associated with unbalanced mitochondrial fission, leading to mitochondrial fragmentation, and DNM1 deletion suppressed the oxidative stress sensitivity and shortened lifespan of isc1Δ cells. Moreover, Isc1p and Dnm1p physically interact, suggesting a possible regulatory role for Isc1p in mitochondrial dynamics. Overall, our work demonstrates that Isc1p-mediated ceramide signalling regulates mitophagy and mitochondrial dynamics in yeast with impact on mitochondrial function and lifespan. Since ceramides have been implicated in ageing and diseases associated with mitochondrial dysfunction, our findings suggest that therapeutic strategies targeting ceramide signalling may improve mitochondrial function and human healthspan., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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21. Accuracy and repeatability of 3 apex locators in locating root canal perforations: an ex vivo study.
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D'Assunção FL, Sousa JC, Felinto KC, de Medeiros TC, Leite DT, de Lucena RB, and de Oliveira Lima J
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- Composite Resins chemistry, Dental Pulp Cavity diagnostic imaging, Humans, Image Processing, Computer-Assisted methods, Materials Testing, Odontometry statistics & numerical data, Photography methods, Radiography, Bitewing methods, Reproducibility of Results, Root Canal Preparation statistics & numerical data, Tooth Apex diagnostic imaging, Dental Pulp Cavity injuries, Electrical Equipment and Supplies statistics & numerical data, Odontometry instrumentation, Root Canal Preparation instrumentation, Tooth Apex anatomy & histology
- Abstract
Introduction: This study was an ex vivo evaluation of the accuracy and repeatability of 3 electronic apex locators (EALs) in locating simulated root canal perforations., Methods: Thirty-five human teeth were used. Perforations were placed 5 mm from the apex of each specimen. A #15 K-file was attached to a micrometer, and the specimens were fixed in a mounting model. The EALs were connected to the files, and electronic measurements of the simulated perforations were obtained. This procedure was repeated, the files were cemented in place, and x-rays were obtained to evaluate the distances between the measurement files and the simulated perforations. The obtained results were compared with the corresponding control lengths. Three investigators evaluated the distance of the file position in relation to the perforation for each specimen. The 3 investigators reached consensus regarding these measurements., Results: Statistical analyses of the results revealed that the EAL reliabilities in the exact detections of the root canal perforations were 97.1% for the Mini Apex Locator, 100% for the Root SW, and 91.4% for the Root ZX II. Analysis of variance revealed that the accuracies of the 3 devices were not different (P > .05). The results also indicated high levels of repeatability for each of the 3 EALs (intraclass correlation coefficients: 0998, 0.999, and 0.999 for the Mini Apex Locator, Root SW, and Root ZX II, respectively)., Conclusions: It was concluded that each of these 3 EALs provided excellent ex vivo accuracy in locating root perforations and that all tested devices possess excellent ex vivo repeatability., (Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
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- 2014
- Full Text
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22. Reduced TORC1 signaling abolishes mitochondrial dysfunctions and shortened chronological lifespan of Isc1p-deficient cells.
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Teixeira V, Medeiros TC, Vilaça R, Moradas-Ferreira P, and Costa V
- Abstract
The target of rapamycin (TOR) is an important signaling pathway on a hierarchical network of interacting pathways regulating central biological processes, such as cell growth, stress response and aging. Several lines of evidence suggest a functional link between TOR signaling and sphingolipid metabolism. Here, we report that the TORC1-Sch9p pathway is activated in cells lacking Isc1p, the yeast orthologue of mammalian neutral sphingomyelinase 2. The deletion of TOR1 or SCH9 abolishes the premature aging, oxidative stress sensitivity and mitochondrial dysfunctions displayed by isc1 Δ cells and this is correlated with the suppression of the autophagic flux defect exhibited by the mutant strain. The protective effect of TOR1 deletion, as opposed to that of SCH9 deletion, is not associated with the attenuation of Hog1p hyperphosphorylation, which was previously implicated in isc1 Δ phenotypes. Our data support a model in which Isc1p regulates mitochondrial function and chronological lifespan in yeast through the TORC1-Sch9p pathway although Isc1p and TORC1 also seem to act through independent pathways, as isc1 Δ tor1 Δ phenotypes are intermediate to those displayed by isc1 Δ and tor1 Δ cells. We also provide evidence that TORC1 downstream effectors, the type 2A protein phosphatase Sit4p and the AGC protein kinase Sch9p, integrate nutrient and stress signals from TORC1 with ceramide signaling derived from Isc1p to regulate mitochondrial function and lifespan in yeast. Overall, our results show that TORC1-Sch9p axis is deregulated in Isc1p-deficient cells, contributing to mitochondrial dysfunction, enhanced oxidative stress sensitivity and premature aging of isc1 Δ cells., Competing Interests: Conflict of interest: The authors declare no conflict of interest.
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- 2014
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23. Essential oil of Croton argyrophylloides: toxicological aspects and vasorelaxant activity in rats.
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de França-Neto A, Cardoso-Teixeira AC, Medeiros TC, Quinto-Farias Mdo S, Sampaio CM, Coelho-de-Souza AN, Lahlou S, and Leal-Cardoso JH
- Subjects
- Animals, Aorta, Thoracic drug effects, Artemia, Brazil, Croton Oil chemistry, Female, Lethal Dose 50, Male, Mice, Muscle Contraction drug effects, Oils, Volatile chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Plant Extracts toxicity, Rats, Rats, Wistar, Splanchnic Circulation drug effects, Vasodilator Agents chemistry, Croton chemistry, Croton Oil pharmacology, Croton Oil toxicity, Oils, Volatile pharmacology, Oils, Volatile toxicity, Vasodilator Agents pharmacology
- Abstract
Croton argyrophylloides Muell. Arg. is widely used in Brazilian folk medicine to treat diabetes and venereal diseases. This study examined the acute toxicity and cytotoxicity of the essential oil of C. argyrophylloides (EOCA). In addition, vascular effects of the EOCA have been examined. In mice, an oral acute toxicity test revealed that EOCA could be considered as a non toxic essential oil since it showed a very high LD50 (9.84 +/- 0.01 g/kg). In the brine shrimp (Artemia salina) cytotoxic assay, the LC50 value of EOCA was 275 [165-534] microg/mL. EOCA (1-1000 microg/mL) relaxed isolated endothelium-intact aortic rings precontracted with phenylephrine with an IC50 value of 126.7 [89.8-163.7] microg/mL. In rat mesenteric bed preparations precontracted with phenylephrine, EOCA (1-300 microg/mL) also induced a reversible, vasodilator effect with an IC50 value of 46.0 [33.3-58.7] micro/mL. It is concluded that EOCA is a very interesting agent from the point of view of the possibility of therapeutic application. This is because, whilst showing a very small acute toxicity, EOCA also showed maximal efficacy as a vascular antispasmodic agent with a pharmacological potency similar to that of other Croton species essential oils.
- Published
- 2012
24. Prospective study in periodontal maintenance therapy: comparative analysis between academic and private practices.
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Costa FO, Santuchi CC, Lages EJ, Cota LO, Cortelli SC, Cortelli JR, Lorentz TC, and Costa JE
- Subjects
- Adolescent, Adult, Aged, Cohort Studies, Dental Clinics, Dental Plaque Index, Diabetes Complications, Disease Progression, Educational Status, Female, Follow-Up Studies, Furcation Defects prevention & control, Gingival Hemorrhage prevention & control, Humans, Income, Male, Middle Aged, Patient Compliance, Periodontal Attachment Loss prevention & control, Periodontal Pocket prevention & control, Prospective Studies, Recurrence, Risk Assessment, Smoking, Tooth Loss etiology, Young Adult, Academic Medical Centers, Chronic Periodontitis prevention & control, Private Practice
- Abstract
Background: This prospective study aims to evaluate and compare the periodontal status, periodontitis progression, tooth loss, and influence of predictable risk variables of two periodontal maintenance therapy programs over a 12-month period., Methods: A total of 288 individuals diagnosed with chronic moderate-to-advanced periodontitis, who had finished active periodontal treatment, were evaluated in a public academic environment (AG) (n = 138), as well as in a private clinic (PG) (n = 150). A full-mouth periodontal examination was performed at baseline and at quadrimestral recalls, evaluating plaque index, probing depth, clinical attachment level, furcation involvement, bleeding on probing (BOP), and suppuration. Individuals' social, demographic, and biologic data, as well as compliance with recalls, were recorded. The effect of variables of interest and confounders were tested by univariate and multivariate analysis., Results: The PG demonstrated lower rates of periodontitis progression and tooth loss than did the AG. After adjusting for confounders, the risk variables of BOP (P = 0.047), smoking (P = 0.003), and diabetes (P = 0.028) for the PG and smoking (P = 0.047) for the AG showed a negative influence on periodontal status., Conclusions: In both groups, the periodontal maintenance therapy minimized the negative effect of the risk variables. However, PG showed significantly less progression of periodontitis and tooth loss compared to AG.
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- 2012
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25. Periodontal risk assessment model in a sample of regular and irregular compliers under maintenance therapy: a 3-year prospective study.
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Costa FO, Cota LO, Lages EJ, Lima Oliveira AP, Cortelli SC, Cortelli JR, Lorentz TC, and Costa JE
- Subjects
- Adolescent, Adult, Age Factors, Alveolar Bone Loss prevention & control, Cohort Studies, Dental Plaque Index, Diabetes Complications, Disease Progression, Female, Follow-Up Studies, Gingival Hemorrhage prevention & control, Humans, Logistic Models, Longitudinal Studies, Male, Middle Aged, Periodontal Attachment Loss prevention & control, Periodontal Pocket prevention & control, Prospective Studies, Recurrence, Risk Assessment, Smoking, Tooth Loss etiology, Young Adult, Chronic Periodontitis prevention & control, Patient Compliance
- Abstract
Background: The purpose of this study is to investigate the association of the periodontal risk assessment (PRA) model with the recurrence of periodontitis and tooth loss during periodontal maintenance therapy (PMT)., Methods: In a prospective PMT program, 75 regular complier (RC) and 89 erratic complier (EC) patients were selected. A periodontal examination and PRA were performed after active periodontal therapy and after 3 years of PMT. Risk profiles (low, moderate, or high) of participants were evaluated, and the recurrence of periodontitis and tooth loss were analyzed using univariate and multivariate analyses., Results: RCs showed less recurrence of periodontitis and tooth loss than ECs (P <0.05). Rates of periodontitis recurrence in RCs and ECs were 2.7% and 3.4%, respectively, for the moderate-risk profile and 6.7% and 11.2%, respectively, for the high-risk profile. During PMT, 49 teeth (0.65 ± 1.4 teeth per participant) were lost in the RC group, and 70 teeth (0.78 ± 2.1 teeth per participant) were lost in the EC group. High-risk profile participants showed more recurrence of periodontitis and lost significantly more teeth than did participants with moderate- or low-risk profiles in RC and EC groups (P <0.05)., Conclusion: The risk profile influenced the recurrence of periodontitis and tooth loss. RCs had less recurrence of periodontitis and less tooth loss. The PRA model can be useful in particularizing the risk of patients and adjusting recall intervals.
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- 2012
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26. Clinical Evidence of the Efficacy of a Mouthwash Containing Propolis for the Control of Plaque and Gingivitis: A Phase II Study.
- Author
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Pereira EM, da Silva JL, Silva FF, De Luca MP, Ferreira EF, Lorentz TC, and Santos VR
- Abstract
The aim of this study was to evidence the clinical efficacy of an alcohol-free mouthwash containing 5.0% (W/V) Brazilian green propolis (MGP 5%) for the control of plaque and gingivitis. Twenty five subjects, men and women aging between 18 and 60 years old (35 ± 9), were included in a clinical trials phase II study who had a minimum of 20 sound natural teeth, a mean plaque index of at least 1.5 (PI), and a mean gingival index of at least 1.0 (GI). They were instructed to rinse with 10 mL of mouthwash test for 1 minute, immediately after brushing in the morning and at night. After 45 and 90 days using mouthwash, the results showed a significant reduction in plaque and in gingival index when compared to samples obtained in baseline. These reductions were at 24% and 40%, respectively (P < .5). There were no important side effects in soft and hard tissues of the mouth. In this study, the MGP 5% showed evidence of its efficacy in reducing PI and GI. However, it is necessary to perform a clinical trial, double-blind, randomized to validate such effectiveness.
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- 2011
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27. Tooth loss in individuals under periodontal maintenance therapy: prospective study.
- Author
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Lorentz TC, Cota LO, Cortelli JR, Vargas AM, and Costa FO
- Subjects
- Adolescent, Adult, Age Factors, Aged, Brazil epidemiology, Epidemiologic Methods, Female, Follow-Up Studies, Humans, Male, Middle Aged, Patient Compliance, Risk Factors, Sex Factors, Socioeconomic Factors, Tooth Loss classification, Tooth Loss prevention & control, Treatment Outcome, Young Adult, Periodontics, Periodontitis therapy, Tooth Loss epidemiology
- Abstract
This prospective study aimed to evaluate the incidence, the underlying reasons, and the influence of predictors of risk for the occurrence of tooth loss (TL) in a program of Periodontal Maintenance Therapy (PMT). The sample was composed of 150 complier individuals diagnosed with chronic moderate-severe periodontitis who had finished active periodontal treatment and were incorporated in a program of PMT. Social, demographic, behavioral and biological variables were collected at quarterly recalls, over a 12-month period. The effect of predictors of risk of and confounding for the dependent variable TL was tested by univariate and multivariate analysis, as well as the underlying reasons and the types of teeth lost. During the monitoring period, there was a considerable improvement in periodontal clinical parameters, with a stability of periodontal status in the majority of individuals. Twenty-eight subjects (18.66%) had TL, totaling 47 lost teeth (1.4%). The underlying reasons for TL were: periodontal disease (n = 34, 72.3%), caries (n = 3, 6.4%), prosthetic reasons (n = 9, 19.2%), and endodontic reasons (n = 1, 2.1%). Additionally, subjects with 10% of sites with probing depth between 4 and 6 mm were 5 times more likely to present TL (OR = 5.13, 95% CI 2.04-12.09). In this study, the incidence of TL was small and limited to few individuals. Additionally, gender and severity of periodontitis were significantly associated with TL during the monitoring period.
- Published
- 2010
- Full Text
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28. Prospective study of complier individuals under periodontal maintenance therapy: analysis of clinical periodontal parameters, risk predictors and the progression of periodontitis.
- Author
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Lorentz TC, Cota LO, Cortelli JR, Vargas AM, and Costa FO
- Subjects
- Adolescent, Adult, Aged, Cohort Studies, Disease Progression, Female, Follow-Up Studies, Humans, Logistic Models, Male, Middle Aged, Periodontal Index, Periodontitis pathology, Periodontitis prevention & control, Prospective Studies, Risk Factors, Statistics, Nonparametric, Treatment Outcome, Young Adult, Oral Hygiene, Patient Compliance, Periodontitis therapy
- Abstract
Aim: This prospective study aimed to evaluate the progression of periodontitis and the influence of risk variables among individuals attending a programme of periodontal maintenance treatment in an academic environment., Material and Methods: A total of 150 individuals diagnosed with chronic moderate-advanced periodontitis, and who had finished active periodontal treatment, were incorporated into the periodontal maintenance therapy. Social, demographic and biological variables of interest from subjects were collected at quarterly recalls, over a 12-month period. The effect of variables of interest and confounding on the periodontal status and progression of periodontitis was tested by univariate and multivariate logistic analysis., Results: A total of 130 subjects (86.7%) showed stable periodontal status, whereas 20 subjects (13.3%) presented periodontitis progression. Twenty-eight subjects (18.66%) presented tooth loss that resulted in a total of 47 lost teeth (1.38%). Diabetes was not found to be associated with periodontitis progression (p=0.67). Smoking was significantly associated with a greater progression of periodontitis (OR=2.7, 95% CI 1.01-7.22)., Conclusions: Periodontal maintenance programmes in academic environment can stabilize the periodontal condition obtained after active periodontal therapy as well as control the action of risk variables for the progression of periodontitis.
- Published
- 2009
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29. Association between maternal periodontitis and an increased risk of preeclampsia.
- Author
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Cota LO, Guimarães AN, Costa JE, Lorentz TC, and Costa FO
- Subjects
- Adolescent, Adult, Brazil epidemiology, Case-Control Studies, Comorbidity, Female, Humans, Middle Aged, Periodontal Diseases epidemiology, Periodontal Index, Pregnancy, Prevalence, Risk Factors, Periodontitis epidemiology, Pre-Eclampsia epidemiology, Pregnancy Complications, Infectious epidemiology
- Abstract
Background: Periodontal disease has been considered a systemic exposure implicated in a higher risk of adverse pregnancy outcomes. The aim of the present study was to determine whether maternal periodontitis is associated with an increased risk of preeclampsia., Methods: A case-control study was conducted in a public hospital in Belo Horizonte, Brazil. During the study period, 588 women, aged 14 to 46 years, were deemed eligible and had data available for analysis. Maternal demographic and medical data were collected from medical records. Preeclampsia was defined as blood pressure >140/90 mm Hg and > or =1+ proteinuria after 20 weeks of gestation. A periodontal examination was performed postpartum. Maternal periodontitis was defined as the presence of four or more teeth with one or more sites with a probing depth > or =4 mm and clinical attachment loss > or =3 mm at the same site. The effects of maternal age, chronic hypertension, primiparity, smoking, alcohol use, and number of prenatal visits were analyzed. Adjusted odds ratios (ORs) for preeclampsia were calculated using multivariate logistic regression., Results: The prevalence of periodontitis was 63.9% and preeclampsia was 18.5%. Variables associated with preeclampsia were chronic hypertension (OR = 4.10; 95% confidence interval [CI] = 2.0 to 8.4; P = 0.001), primiparity (OR = 2.40; 95% CI = 1.5 to 3.9; P = 0.004), maternal age (OR = 1.07; 95% CI = 1.0 to 1.1; P = 0.001), and maternal periodontitis (OR = 1.88; 95% CI = 1.1 to 3.0; P = 0.001)., Conclusion: Maternal periodontitis was determined to be associated with an increased risk of preeclampsia.
- Published
- 2006
- Full Text
- View/download PDF
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