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Introduction: Chronic venous disease (CVD) is mostly underdiagnosed diseasewhich may progress to chronic venous insufficiency and venous ulcer thus extensive treatment. CVD may affect negatively into patient’sgood well-beingwhich can create burdens on their life and also healthcare resources. Bangladesh has very minimum awareness on CVD. Materials and Methods: This was a cross-sectional study was carried out in department of vascular surgery of National Institute of Cardiovascular disease. we randomly choose 60 patients with chonic venous disease (CVD) and evaluate their characteristics and prevalence of several types along with the management pattern. Data collection started from May 2021 for next 6 months. Results: The patients of this survey were aged between 24 to 70 years and the Mean±SD age was 43.6±12.2. 78.3% of total study population were male, 33.3% were businessman and 10% were housewife. 66.6% patients were having low socio-economic condition. C2 varicose veins were highly prevalent among the study population ( 35%) & lowest prevalence of C4b lipodermatosclerosis or atrophie blanche (11.7%). Male were predominantly more prevalent to having CVD, 78.33% (n47), (p

Introduction: Arterial catheters include radial, brachial, femoral, dorsalis pedis and axillary artery are used in the intensive care unit for continuous blood pressure monitoring, repeated blood sampling. Normal saline solution can maintain patency of arterial and central venous pressure monitoring catheters. Heparinized solutions had no effect on prolonging patency and improving function of catheters while the normal saline solution increases accuracy of coagulatory tests. Aim of the Study: The aim of this study was to examine the efficacy of Normal saline as flushing solution to maintain indwelling arterial catheter patency among patients after cardiac single valve surgery. Methods: This was a prospective cross-sectional study conducted in the Department of Cardiac surgery of National Institute of Cardiovascular Disease (NICVD), Sher-E-Bangla Nagar, Dhaka, Bangladesh during the period from July 2016 to June 2017. This study included sixty patients who underwent single valve replacement surgery and shifted to the ICU with arterial line catheter. The patients who fulfilled the inclusion criteria were divided into two groups- Group 1 (with heparin solution) and Group 2 (with normal saline). Result: In our study we found the mean ± SD of age among Group 1 & 2 was (36.3±6.7) & (35.7±10.3) respectively. We found 100% patients had arterial line patent in 1st POD in both groups. In 2nd POD 86.8% & 76.7% and at 3rd POD 50.0% & 43.3% patients had patency in Group 1 & Group 2 respectively. We found the mean± SD of platelet count at 1st POD was (206530±64441) & (212543±48768); at 3rd POD was (226517±60185) & (245957±52826); at 7th POD was (240517±57379) & (257713±53655); at 14th POD was (245850±52680) & (244337±56796) in Group 1 & 2 respectively which was significantly higher in Group 2. Conclusion: The use of heparin in normal saline for arterial line flushing does not significantly reduce platelet counts in cardiac valve surgery patients in comparison to use of normal ....

Introduction: Arterial catheters are widely used in intensive care units for continuous blood pressure monitoring and blood sampling. The patency of these lines is maintained by continuous flushing, usually with the addition of the anticoagulant heparin to the flushing solution. Normal saline solution can maintain patency of arterial and central venous pressure monitoring catheters. Aim of the Study: The aim of this study was to evaluate the effect between normal saline and heparin solution in arterial line flushing on platelet count. Methods: This was a prospective cross- sectional study conducted in the Department of Cardiac surgery of National Institute of Cardiovascular Disease (NICVD), Sher-E-Bangla Nagar, Dhaka, Bangladesh during the period from July 2016 to June 2017. This study included sixty patients who underwent single valve replacement surgery and shifted to the ICU with arterial line catheter. The patients who fulfilled the inclusion criteria were divided into two groups- Group A (with heparin solution) and Group B (with normal saline). Result: In total 60 patients from both the groups completed the study. In our study we found the mean ± SD of age among group A & B was (36.3±6.7) & (35.7±10.3) respectively. We found the mean± SD of platelet count at 1st POD was (206530±64441) & (212543±48768); at 3rd POD was (226517±60185) & (245957±52826); at 7th POD was (240517±57379) & (257713±53655); at 14th POD was (245850±52680) & (244337±56796) in Group A & B respectively. Conclusion: In our study we didn’t find any statistically significant difference regarding the patients’ demographic characteristics and there were no statistically significant difference of platelet counts between Group A and Group B at preoperative period, post pump, 1st POD, 2nd POD, 3rd POD, 4th POD, 7th POD and 14th POD.

The NanoLuc luciferase (NLuc) and its furimazine (FRZ) substrate have revolutionized bioluminescence (BL) assays and imaging. However, the use of the NLuc-FRZ luciferase-luciferin pair for mammalian tissue imaging is hindered by the low tissue penetration of the emitting blue photons. Here, we present the development of an NLuc mutant, QLuc, which catalyzes the oxidation of a synthetic QTZ luciferin for bright and red-shifted emission peaking at ∼585 nm. Compared to other small single-domain NLuc mutants, this amber-light-emitting luciferase exhibited improved performance for imaging deep-tissue targets in live mice. Leveraging this novel bioluminescent reporter, we further pursued

This paper proposes a fast and robust technique for instantaneous phase angle estimation of three-phase voltage systems under unbalanced and distorted conditions. It mainly relies on the Clarke transformation, Teager energy operator and delayed signal cancellation (DSC) approach. Unlike an adaptive DSC-based synchronous-reference frame phase-locked loop (PLL), the proposed technique avoids interdependent loops, thus increasing the overall stability and easing the tuning process. Moreover, it removes the phase loop of the PLL, hence eliminates the requirement of in-loop filter (low-pass filter) and/or loop filter (proportional plus integral controller). As a result, the tuning of these filters is not required and a quicker response is produced. The proposed technique also avoids inverse trigonometric function operation. In addition, it shows harmonics immunity at steady-state and can generate fast dynamic response with a settling time less than one nominal fundamental cycle. Both simulation and experimental results are presented to reveal the benefits of the technique.

This paper proposes an open-loop based demodulation technique for the instantaneous phase angle estimation of three phase voltage systems. The proposed demodulation-based method relies on the Clarke transform, finite-impulse-response filters and algorithms for phase angle and frequency deviation estimation. When compared to a frequency-locked loop (FLL) based on dual second-order generalized integrators and a phase-locked loop (PLL) relying on adaptive delayed signal cancellation operator, the proposed one does not use any integrators, recursive signals and feedback of frequency or phase angle, thus unconditionally stable and relatively simple tuning is achieved. In addition, the proposed method is computationally efficient due to avoiding interpolation, square-root, trigonometric functions or their inverse operations. Moreover, under equal dynamic condition, the proposed one generates improved accuracy at steady-state in a range of the frequency deviation specified by standard and grid code. Simulated and experimental results are presented to verify the efficacy of the proposed method.

This letter proposes a three-phase phase-locked loop (PLL) algorithm relying on an adaptive Clarke transform (CT) under amplitude and/or phase angle unbalanced condition. Analytical expressions for coefficients of matrix associated with the adaptive CT are derived and used to generate orthogonal signals. An auxiliary algorithm is also proposed to track the actual amplitudes and phase angle deviations. Unlike the conventional CT relying on constant coefficients, the adaptive CT can generate accurate orthogonal signals from the three-phase voltages including phase angle and/or amplitude imbalances. As a result, the proposed PLL is not affected by the unbalanced phase angles with/without amplitude imbalances. The advantages of the proposed adaptive CT based PLL method is illustrated by both simulation and experimental results.

Liver disease indicates inflammatory condition of the liver, liver cirrhosis, cancer, or an overload of toxic substances. A liver transplant may reinstate and extend life if a patient has severe liver disease. In the last few years, machine learning (ML) based diagnosis systems have played a vital role in assessing liver patients which eventually leads to proper treatment and saves human life. In this study, we try to predict liver patients by adopting a hybrid feature extraction method to enhance the performance of the ML algorithm. Medical data frequently exhibits non-linear patterns and class imbalances. This is undesirable for the majority of ML algorithms and degrades performance. Here, we present a hybrid feature space that combines t-SNE, Isomap nonlinear features, and kernel principal components that can explain 90% of the variation in the data as a solution to this issue. Before feeding the ML model, data preprocessing techniques including class balancing, identifying outliers, and impute missing values are used. A simulation study and ensemble learning also conducted to justify the proposed prediction performances. Our suggested hybrid non-linear feature exhibits a 2-20 % improvement over existing studies and the ensemble classifier achieved an ideal and outstanding accuracy of 91.33 %.

This letter proposes a method for fast and accurate estimation of three-phase grid frequency under unbalanced and distorted conditions. It includes a recursive discrete Fourier transform-based frequency adaptive bandpass filter for α – β signals generated by the Clarke transform and a two-point-based algorithm for frequency estimation. The proposed method is relatively simple to implement and can eliminate adverse influences created by the imbalances, dc offset and both odd and even harmonics. It can also provide fast estimation with a response time of almost one fundamental cycle under grid disturbances. Moreover, it can present improved performance when compared to several methods reported in the technical literature. The advantages of the proposed method are confirmed by both simulated and real-time experimental results.

Revised Supplementary Materials and Methods and Supplementary Figure Legends

Supplementary Figures S1-S9. Figure S1. Functional evaluation of the GpNLuc LumiFluor. Figure S2. In vitro characterization of FACS sorted mouse Em-Myc lymphoma and human non-small cell lung cancer (NSCLC) cells. Figure S3. Temporal development of A549 NSCLC xenograft tumors expressing GpNLuc or GpNLuc and the tumor suppressor LKB1. Figure S4. Detection of spontaneous lymph node metastasis from primary lung tumors. Figure S5. In vitro and in vivo characterization of independently derived E?-Myc lymphoma cells stably expressing GpNLuc. Figure S6. Bioluminescence imaging of E?-Myc lymphoma-GpNLuc allografts. Figure S7. A, in vivo dose-response kinetics of GpNLuc signal strength. Figure S8. Quantification of in vivo bioluminescence imaging and confirmation ex vivo. Figure S9. Flow cytometric analysis of inducibly expressed fluorescent proteins in stable lymphoma cell lines.

Living Image DLIT 3D reconstruction of Emu-Myc Lymphoma-GpNLuc orthotopic allograft transplant.

Background: Homocysteine is increasingly recognized as an independent risk factor for vascular disease specially peripheral arterial occlusive diseases. In the present study, an attempt was made to find out the association between the raised fasting plasma total homocysteine level and early onset atherosclerotic peripheral arterial occlusive diseases (PAOD). Methods: In this prospective observational study a total of 50 study subjects were included. All patients were clinically and angiographically documented for atherosclerotic PAOD. All patients underwent surgical intervention as well as estimation of serum total homocysteine level. Arterial segment was sent for histopathological examination to find out whether atherosclerosis was present or not. Patients were divided into two groups- Group-1 included patients of 20-40 years of age and group-II included those of 41-60 years of age. The groups were compared to see association between elevated level of plasma homocysteine and atherosclerotic peripheral arterial occlusive diseases in elderly as well as in early age. Results: 41 (82%) patients were male and 9 (18%) patients were female. Serum homocysteine level was higher in group I than group II (71.4% vs. 40.9%). Besides, the level of mean serum homocysteine level was significantly (p=0.02) higher in group I than group II (21.18±9.53 vs. 17.24±8.92 μmol/L). Conclusion: In conclusion, this study suggests that serum homocysteine has an association with early onset atherosclerotic PAOD. Therefore, a raised serum homocysteine level can be used as an independent biochemical predictor of early onset atherosclerotic PAOD. Cardiovasc. j. 2021; 13(2): 120-127

The NanoLuc luciferase (NLuc) and its furimazine (FRZ) substrate have revolutionized bioluminescence (BL) assays and imaging. However, the use of the NLuc-FRZ luciferase-luciferin pair for mammalian tissue imaging is hindered by the low tissue penetration of the emitting blue photons. Here, we present the development of an NLuc mutant, QLuc, which catalyzes the oxidation of a synthetic QTZ luciferin for bright and red-shifted emission peaking at ∼ 585 nm. This amber-light-emitting luciferase-luciferin pair exhibited improved performance for imaging deep-tissue targets in live mice. Leveraging this novel bioluminescent reporter, we further pursued in vivo immunobioluminescence imaging (immunoBLI), which used a fusion protein of a single-chain variable antibody fragment (scFv) and QLuc for molecular imaging of tumor-associated antigens in a xenograft mouse model. As one of the most red-shifted NLuc variants, we expect QLuc to find broad applications in noninvasive imaging in mammals. Moreover, the immunoBLI method complements immunofluorescence imaging and immuno-positron emission tomography (immunoPET), serving as a convenient and nonradioactive molecular imaging tool for animal models in basic and preclinical research.