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The condition of hyperlipidemia is found to be a great establisher for all the negative health consequences which may lead to cardiac complications. Continuous usage of medication alone is not permanent remedy but also need physical exercise. The same situation was established in animals to assess the performance of pharmacological and non-pharmacological profile by standardized screening using In-vivo methods. The high-cholesterol diet caused a significant increase in total lipids, total cholesterol (TC), total triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and the atherogenic index, whereas the level of high-density lipoprotein cholesterol (HDL-C) was significantly decreased for the treatment groups intended for physical exercise. This article enables the importance of health benefited by physical performance by depicting the biochemical parameters. Keywords: Hyperlipidemia, Triglycerides, low-density lipoprotein, activity wheel, Physical exercise, Cardiac performance.