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Your search keyword '"Mckay, Ronald D. G."' showing total 166 results
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166 results on '"Mckay, Ronald D. G."'

1. Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs

Author

Burke, Emily E., Chenoweth, Joshua G., Shin, Joo Heon, Collado-Torres, Leonardo, Kim, Suel-Kee, Micali, Nicola, Wang, Yanhong, Colantuoni, Carlo, Straub, Richard E., Hoeppner, Daniel J., Chen, Huei-Ying, Sellers, Alana, Shibbani, Kamel, Hamersky, Gregory R., Diaz Bustamante, Marcelo, Phan, BaDoi N., Ulrich, William S., Valencia, Cristian, Jaishankar, Amritha, Price, Amanda J., Rajpurohit, Anandita, Semick, Stephen A., Bürli, Roland W., Barrow, James C., Hiler, Daniel J., Page, Stephanie C., Martinowich, Keri, Hyde, Thomas M., Kleinman, Joel E., Berman, Karen F., Apud, Jose A., Cross, Alan J., Brandon, Nicholas J., Weinberger, Daniel R., Maher, Brady J., McKay, Ronald D. G., and Jaffe, Andrew E.

Published
2020
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2. Human ES cell lines—introduction

Author

Andrews, Peter W., Benvenisty, Nissim, Knowles, Barbara B., McKay, Ronald D. G., Oh, Steve K. W., Pera, Martin F., Rossant, Janet, and Stacey, Glyn N.

Published
2010

3. Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells

Author

Akopian, Veronika, Andrews, Peter W., Beil, Stephen, Benvenisty, Nissim, Brehm, Jennifer, Christie, Megan, Ford, Angela, Fox, Victoria, Gokhale, Paul J., Healy, Lyn, Holm, Frida, Hovatta, Outi, Knowles, Barbara B., Ludwig, Tenneille E., McKay, Ronald D. G., Miyazaki, Takamichi, Nakatsuji, Nono, Oh, Steve K. W., Pera, Martin F., Rossant, Janet, Stacey, Glyn N., and Suemori, Hirofumi

Published
2010

5. A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells

Author

Tsai, Robert Y. L. and McKay, Ronald D. G.

Subjects
Genetic research -- Analysis, Cell proliferation -- Genetic aspects, Cell proliferation -- Research, Stem cells -- Genetic aspects, Cancer cells -- Genetic aspects, Biological sciences
Abstract

Research has been conducted on stem and cancer cells. Results demonstrate that nucleostemin controls stem and cancer cell prolioferation.

Published
2002

17. A Role for Sox2 in the Adult Cerebellum

Author

Mandalos, Nikolaos Panagiotis Karampelas, Ioannis Saridaki, Marannia McKay, Ronald D. G. Cohen, Mark L. Remboutsika, Eumorphia

Subjects
nervous system, embryonic structures
Abstract

The cerebellum, a derivative of the hindbrain, plays a crucial role in balance and posture as well as in higher cognitive and locomotive processes. Cerebellar development is initiated during the segmental phase of hindbrain formation. Here, we describe the phenotype, of a single surviving adult conditional mouse mutant mouse, in which Sox2 function is ablated in embryonic radial glial cells by means of hGFAP-CRE. The single Sox2(RGINV/mosaic) adult mutant mouse displays motor disability, microsomia, reduced Central Nervous System (CNS) size and cerebellar defects associated with human genetically related congenital abnormalities.

Published
2018

19. A Role for Sox2 in the Adult Cerebellum

Author

Mandalos, Nikolaos Panagiotis, primary, Karampelas, Ioannis, additional, Saridaki, Marannia, additional, McKay, Ronald D G, additional, Cohen, Mark L, additional, and Remboutsika, Eumorphia, additional

Published
2018
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20. A 'Hit and Run' Approach to Inducible Direct Reprogramming of Astrocytes to Neural Stem Cells

Author

Poulou, Maria Mandalos, Nikolaos P. Karnavas, Theodoros and Saridaki, Marannia McKay, Ronald D. G. Remboutsika, Eumorphia

Subjects
embryonic structures, fungi
Abstract

Temporal and spatial control of gene expression can be achieved using an inducible system as a fundamental tool for regulated transcription in basic, applied and eventually in clinical research. We describe a novel ``hit and run' inducible direct reprogramming approach. In a single step, 2 days post-transfection, transiently transfected Sox2(FLAG) under the Leu3p-alpha IPM inducible control (iSox2) triggers the activation of endogenous Sox2, redirecting primary astrocytes into abundant distinct nestin-positive radial glia cells. This technique introduces a unique novel tool for safe, rapid and efficient reprogramming amendable to regenerative medicine.

Published
2016

22. Creating Patient-Specific Neural Cells for the in Vitro Study of Brain Disorders

Author

Brennand, Kristen Jennifer, McConnell, Michael M.J., McKay, Ronald D G R.D., Morrow, Eric E.M., Muotri, Alysson Renato, Panchision, David D.M., Rubin, Lee L.L., Sawa, Akira, Soldner, Frank, Song, Hongjun, Studer, Lorenz, Marchetto, Maria Carol Arolina M.C., Temple, Sally, Vaccarino, Flora F.M., Wu, Junxi, Vanderhaeghen, Pierre, Gage, Fred H F.H., Jaenisch, Rudolf, Benvenisty, Nissim, Brüstle, Oliver, Ebert, Allison, Izpisua Belmonte, Juan Carlos, Kaykas, Ajamete, Lancaster, Madeline M.A., Livesey, Frederick F.J., Brennand, Kristen Jennifer, McConnell, Michael M.J., McKay, Ronald D G R.D., Morrow, Eric E.M., Muotri, Alysson Renato, Panchision, David D.M., Rubin, Lee L.L., Sawa, Akira, Soldner, Frank, Song, Hongjun, Studer, Lorenz, Marchetto, Maria Carol Arolina M.C., Temple, Sally, Vaccarino, Flora F.M., Wu, Junxi, Vanderhaeghen, Pierre, Gage, Fred H F.H., Jaenisch, Rudolf, Benvenisty, Nissim, Brüstle, Oliver, Ebert, Allison, Izpisua Belmonte, Juan Carlos, Kaykas, Ajamete, Lancaster, Madeline M.A., and Livesey, Frederick F.J.

Abstract

As a group, we met to discuss the current challenges for creating meaningful patient-specific in vitro models to study brain disorders. Although the convergence of findings between laboratories and patient cohorts provided us confidence and optimism that hiPSC-based platforms will inform future drug discovery efforts, a number of critical technical challenges remain. This opinion piece outlines our collective views on the current state of hiPSC-based disease modeling and discusses what we see to be the critical objectives that must be addressed collectively as a field., SCOPUS: cp.j, info:eu-repo/semantics/published

Published
2015

23. Androutsellis-Theotokis, A. et al. Notch signalling regulates stem cell numbers in vitro and in vivo. Nature 442, 823-826

Author

Androutsellis-Theotokis, Andreas, Leker, Ronen R., Soldner, Frank, Hoeppner, Daniel J., Ravin, Rea, Poser, Steve W., Rueger, Maria A., Bae, Soo-Kyung, Kittappa, Raja, and McKay, Ronald D. G.

Abstract

The hope of developing new transplantation therapies for degenerative diseases is limited by inefficient stem cell growth and immunological incompatibility with the host. Here we show that Notch receptor activation induces the expression of the specific target genes hairy and enhancer of split 3 (Hes3) and Sonic hedgehog (Shh) through rapid activation of cytoplasmic signals, including the serine/threonine kinase Akt, the transcription factor STAT3 and mammalian target of rapamycin, and thereby promotes the survival of neural stem cells. In both murine somatic and human embryonic stem cells, these positive signals are opposed by a control mechanism that involves the p38 mitogen-activated protein kinase. Transient administration of Notch ligands to the brain of adult rats increases the numbers of newly generated precursor cells and improves motor skills after ischaemic injury. These data indicate that stem cell expansion in vitro and in vivo, two central goals of regenerative medicine, may be achieved by Notch ligands through a pathway that is fundamental to development and cancer.

Published
2006

24. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Author

Adewumi, Oluseun, Aflatoonian, Behrouz, Ahrlund-Richter, Lars, Amit, Michal, Andrews, Peter W., Beighton, Gemma, Bello, Paul A., Benvenisty, Nissim, Berry, Lorraine S., Bevan, Simon, Blum, Barak, Brooking, Justin, Chen, Kevin G., Choo, Andre B. H., Churchill, Gary A., Corbel, Marie, Damjanov, Ivan, Draper, Jon S., Dvorak, Petr, Emanuelsson, Katarina, Fleck, Roland A., Ford, Angela, Gertow, Karin, Gertsenstein, Marina, Gokhale, Paul J., Hamilton, Rebecca S., Hampl, Ales, Healy, Lyn E., Hovatta, Outi, Hyllner, Johan, Imreh, Marta P., Itskovitz-Eldor, Joseph, Jackson, Jamie, Johnson, Jacqueline L., Jones, Mark, Kee, Kehkooi, King, Benjamin L., Knowles, Barbara B., Lako, Majlinda, Lebrin, Franck, Mallon, Barbara S., Manning, Daisy, Mayshar, Yoav, Mckay, Ronald D. G., Michalska, Anna E., Mikkola, Milla, Mileikovsky, Masha, Minger, Stephen L., Moore, Harry D., Mummery, Christine L., Nagy, Andras, Nakatsuji, Norio, O'Brien, Carmel M., Oh, Steve K. W., Olsson, Cia, Otonkoski, Timo, Park, Kye-Yoon, Passier, Robert, Patel, Hema, Patel, Minal, Pedersen, Roger, Pera, Martin F., Piekarczyk, Marian S., Pera, Renee A. Reijo, Reubinoff, Benjamin E., Robins, Allan J., Rossant, Janet, Rugg-Gunn, Peter, Schulz, Thomas C., Semb, Henrik, Sherrer, Eric S., Siemen, Henrike, Stacey, Glyn N., Stojkovic, Miodrag, Suemori, Hirofumi, Szatkiewicz, Jin, Turetsky, Tikva, Tuuri, Timo, van den Brink, Steineke, Vintersten, Kristina, Vuoristo, Sanna, Ward, Dorien, Weaver, Thomas A., Young, Lesley A., Zhang, Weidong, Adewumi, Oluseun, Aflatoonian, Behrouz, Ahrlund-Richter, Lars, Amit, Michal, Andrews, Peter W., Beighton, Gemma, Bello, Paul A., Benvenisty, Nissim, Berry, Lorraine S., Bevan, Simon, Blum, Barak, Brooking, Justin, Chen, Kevin G., Choo, Andre B. H., Churchill, Gary A., Corbel, Marie, Damjanov, Ivan, Draper, Jon S., Dvorak, Petr, Emanuelsson, Katarina, Fleck, Roland A., Ford, Angela, Gertow, Karin, Gertsenstein, Marina, Gokhale, Paul J., Hamilton, Rebecca S., Hampl, Ales, Healy, Lyn E., Hovatta, Outi, Hyllner, Johan, Imreh, Marta P., Itskovitz-Eldor, Joseph, Jackson, Jamie, Johnson, Jacqueline L., Jones, Mark, Kee, Kehkooi, King, Benjamin L., Knowles, Barbara B., Lako, Majlinda, Lebrin, Franck, Mallon, Barbara S., Manning, Daisy, Mayshar, Yoav, Mckay, Ronald D. G., Michalska, Anna E., Mikkola, Milla, Mileikovsky, Masha, Minger, Stephen L., Moore, Harry D., Mummery, Christine L., Nagy, Andras, Nakatsuji, Norio, O'Brien, Carmel M., Oh, Steve K. W., Olsson, Cia, Otonkoski, Timo, Park, Kye-Yoon, Passier, Robert, Patel, Hema, Patel, Minal, Pedersen, Roger, Pera, Martin F., Piekarczyk, Marian S., Pera, Renee A. Reijo, Reubinoff, Benjamin E., Robins, Allan J., Rossant, Janet, Rugg-Gunn, Peter, Schulz, Thomas C., Semb, Henrik, Sherrer, Eric S., Siemen, Henrike, Stacey, Glyn N., Stojkovic, Miodrag, Suemori, Hirofumi, Szatkiewicz, Jin, Turetsky, Tikva, Tuuri, Timo, van den Brink, Steineke, Vintersten, Kristina, Vuoristo, Sanna, Ward, Dorien, Weaver, Thomas A., Young, Lesley A., and Zhang, Weidong

Abstract

The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

Published
2007
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25. Regulation and Expression of the ATP-Binding Cassette Transporter ABCG2 in Human Embryonic Stem Cells

Author

Padmanabhan, Raji, primary, Chen, Kevin G., additional, Gillet, Jean-Pierre, additional, Handley, Misty, additional, Mallon, Barbara S., additional, Hamilton, Rebecca S., additional, Park, Kyeyoon, additional, Varma, Sudhir, additional, Mehaffey, Michele G., additional, Robey, Pamela G., additional, McKay, Ronald D. G., additional, and Gottesman, Michael M., additional

Published
2012
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27. Angiogenic Factors Stimulate Growth of Adult Neural Stem Cells

Author

Androutsellis-Theotokis, Andreas, primary, Rueger, Maria A., additional, Park, Deric M., additional, Boyd, Justin D., additional, Padmanabhan, Raji, additional, Campanati, Loraine, additional, Stewart, Craig V., additional, LeFranc, Yann, additional, Plenz, Dietmar, additional, Walbridge, Stuart, additional, Lonser, Russell R., additional, and McKay, Ronald D. G., additional

Published
2010
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31. Identification and Characterization of Cell Type–Specific and Ubiquitous Chromatin Regulatory Structures in the Human Genome

Author

Xi, Hualin, primary, Shulha, Hennady P, additional, Lin, Jane M, additional, Vales, Teresa R, additional, Fu, Yutao, additional, Bodine, David M, additional, McKay, Ronald D. G, additional, Chenoweth, Josh G, additional, Tesar, Paul J, additional, Furey, Terrence S, additional, Ren, Bing, additional, Weng, Zhiping, additional, and Crawford, Gregory E, additional

Published
2007
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47. Isolation of Epiblast Stem Cells from Preimplantation Mouse Embryos.

Author

Najm, Fadi J., Chenoweth, Josh G., Anderson, Philip D., Nadeau, Joseph H., Redline, Raymond W., McKay, Ronald D. G., and Tesar, Paul J.

Subjects
STEM cells, CELL separation, PREIMPLANTATION genetic diagnosis, EMBRYONIC stem cells, MICE anatomy, DISEASE progression
Abstract

Pluripotent stem cells provide a platform to interrogate control elements that function to generate all cell types of the body. Despite their utility for modeling development and disease, the relationship of mouse and human pluripotent stem cell states to one another remains largely undefined. We have shown that mouse embryonic stem (ES) cells and epiblast stem cells (EpiSCs) are distinct, pluripotent states isolated from pre- and post-implantation embryos respectively. Human ES cells are different than mouse ES cells and share defining features with EpiSCs, yet are derived from pre-implantation human embryos. Here we show that EpiSCs can be routinely derived from pre-implantation mouse embryos. The preimplantation-derived EpiSCs exhibit molecular features and functional properties consistent with bona fide EpiSCs. These results provide a simple method for isolating EpiSCs and offer direct insight into the intrinsic and extrinsic mechanisms that regulate the acquisition of distinct pluripotent states. [ABSTRACT FROM AUTHOR]

Published
2011
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48. Oxygen Tension Regulates Survival and Fate of Mouse Central Nervous System Precursors at Multiple Levels.

Author

Hui-Ling Chen, Pistollato, Francesca, Hoeppner, Daniel J., Hsiao-Tzu Ni, McKay, Ronald D. G., and Panchision, David M.

Subjects
OXYGEN, CENTRAL nervous system, CELL differentiation, APOPTOSIS, ASTROCYTES, PLATELET-derived growth factor, CELLULAR therapy, ANIMAL models in research
Abstract

Despite evidence that oxygen regulates neural precursor fate, the effects of changing oxygen tensions on distinct stages in precursor differentiation are poorly understood. We found that 5% oxygen permitted clonal and long-term expansion of mouse fetal cortical precursors. In contrast, 20% oxygen caused a rapid decrease in hypoxia-inducible factor 1α and nucleophosmin, followed by the induction of p53 and apoptosis of cells. This led to a decrease in overall cell number and particularly a loss of astrocytes and oligodendrocytes. Clonal analysis revealed that apoptosis in 20% oxygen was due to a complete loss of CD133loCD24lo multipotent precursors, a substantial loss of CD133hiCD24lo multipotent precursors, and a failure of remaining CD133hiCD24lo cells to generate glia. In contrast, committed neuronal progenitors were not significantly affected. Switching clones from 5% to 20% oxygen only after mitogen withdrawal led to a decrease in total clone numbers but an even greater decrease in oligodendrocyte-containing clones. During this late exposure to 20% oxygen, bipotent glial (A2B5+) and early (platelet-derived growth factor receptor α) oligodendrocyte progenitors appeared and disappeared more quickly, relative to 5% oxygen, and late stage O4+ oligodendrocyte progenitors never appeared. These results indicate that multipotent cells and oligodendrocyte progenitors are more susceptible to apoptosis at 20% oxygen than committed neuronal progenitors. This has important implications for optimizing ex vivo production methods for cell replacement therapies. [ABSTRACT FROM AUTHOR]

Published
2007
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49. Identification and Characterization of Cell Type-Specific and Ubiquitous Chromatin Regulatory Structures in the Human Genome.

Author

Hualin Xi, Shulha, Hennady P., Lin, Jane M., Vales, Teresa R., Yutao Fu, Bodine, David M., McKay, Ronald D. G., Chenoweth, Josh G., Tesar, Paul J., Furey, Terrence S., Bing Ren, Zhiping Weng, and Crawford, Gregory E.

Subjects
CHROMATIN, HUMAN genome, GENOMES, TRANSCRIPTION factors, GENE expression
Abstract

The identification of regulatory elements from different cell types is necessary for understanding the mechanisms controlling cell type--specific and housekeeping gene expression. Mapping DNaseI hypersensitive (HS) sites is an accurate method for identifying the location of functional regulatory elements. We used a high throughput method called DNase-chip to identify 3,904 DNaseI HS sites from six cell types across 1% of the human genome. A significant number (22%) of DNaseI HS sites from each cell type are ubiquitously present among all cell types studied. Surprisingly, nearly all of these ubiquitous DNaseI HS sites correspond to either promoters or insulator elements: 86% of them are located near annotated transcription start sites and 10% are bound by CTCF, a protein with known enhancer-blocking insulator activity. We also identified a large number of DNaseI HS sites that are cell type specific (only present in one cell type); these regions are enriched for enhancer elements and correlate with cell type--specific gene expression as well as cell type--specific histone modifications. Finally, we found that approximately 8% of the genome overlaps a DNaseI HS site in at least one the six cell lines studied, indicating that a significant percentage of the genome is potentially functional. [ABSTRACT FROM AUTHOR]

Published
2007
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