Search

Your search keyword '"Mcguinness, Debra"' showing total 19 results
Start Over Author "Mcguinness, Debra"
19 results on '"Mcguinness, Debra"'

3. Preclinical evaluation of an investigational 21-valent pneumococcal conjugate vaccine, V116, in adult-rhesus monkey, rabbit, and mouse models

Author

Curry, Stephanie, primary, Kaufhold, Robin M., additional, Monslow, Morgan A., additional, Zhang, Yuhua, additional, McGuinness, Debra, additional, Kim, Ellie, additional, Nawrocki, Denise K., additional, McHugh, Patrick M., additional, Briggs, Marie L., additional, Smith, William J., additional, He, Jian, additional, Joyce, Joseph G., additional, and Skinner, Julie M., additional

Published
2023
Full Text
View/download PDF

7. Screening for Antiviral Inhibitors of the HIV Integrase--LEDGF/p75 Interaction Using the AlphaScreen Luminescent Proximity Assay

Author

Mcguinness, Debra E., Prongay, Andrew J., Feld, Boris, Ingravallo, Paul, Ogert, Robert A., Lunn, Charles A., and Howe, John A.

Subjects
HIV testing -- Methods -- Health aspects -- Physiological aspects -- Research, Protein-protein interactions -- Physiological aspects -- Research -- Methods -- Health aspects, Integrase inhibitors -- Health aspects -- Research, Biological sciences, Physiological aspects, Research, Methods, Health aspects
Abstract

Keywords: HIV integrase; LEDGF/p75; protein-protein interaction; AlphaScreen; orthogonal mixtures 406 Screening for Antiviral Inhibitors of the HIV Integrase--LEDGF/p75 Interaction Using the AlphaScreen Luminescent Proximity Assay SAGE Publications, Inc.200810.1177/1087057108317060 Yan Hou [...]

Published
2008
Full Text
View/download PDF

8. Assignment of opsonic values to pneumococcal reference serum 007sp and a second pneumococcal OPA calibration serum panel (Ewha QC sera panel B) for 11 serotypes

Author

Burton, Robert L., primary, Kim, Han Wool, additional, Lee, Soyoung, additional, Kim, Hun, additional, Seok, Jee-hyun, additional, Ku, Kun Young, additional, Seo, Jihye, additional, Kim, Sun Jin, additional, Xie, Jinfu, additional, McGuinness, Debra, additional, Skinner, Julie M., additional, Choi, Seuk Keun, additional, Baik, Yeong Ok, additional, Bae, Sejong, additional, Nahm, Moon H., additional, and Kim, Kyung-Hyo, additional

Published
2020
Full Text
View/download PDF

9. Additional file 1: of Human monoclonal antibodies isolated from a primary pneumococcal conjugate Vaccinee demonstrates the expansion of an antigen-driven Hypermutated memory B cell response

Author

Zhifeng Chen, Cox, Kara, Aimin Tang, Roman, Jeanette, Fink, Malorie, Kaufhold, Robin, Liming Guan, Xie, Andy, Boddicker, Melissa, Mcguinness, Debra, Xiao, Xiao, Hualin Li, Skinner, Julie, Verch, Thorsten, Retzlaff, Mary, and Kalpit Vora

Abstract

Figure S1. FACS sorting for single plasmablast cells. Freshly harvested peripheral blood mononuclear cells were stained with antibodies for flow cytometric detection of plasmablast cells. Plasmablasts were gated as shown, defined as lymphocytes/single cells/CD3-/CD19+ and low/CD27hiCD38hi/CD20 low, and sorted into single wells of a 96 well plate for RNA preservation and cloning. (PDF 1246â kb)

Published
2018
Full Text
View/download PDF

10. Additional file 2: of Human monoclonal antibodies isolated from a primary pneumococcal conjugate Vaccinee demonstrates the expansion of an antigen-driven Hypermutated memory B cell response

Author

Zhifeng Chen, Cox, Kara, Aimin Tang, Roman, Jeanette, Fink, Malorie, Kaufhold, Robin, Liming Guan, Xie, Andy, Boddicker, Melissa, Mcguinness, Debra, Xiao, Xiao, Hualin Li, Skinner, Julie, Verch, Thorsten, Retzlaff, Mary, and Kalpit Vora

Abstract

Figure S2. Crystal structure and model of two antibody Fabs with VH3/JH4 gernline usage bound to polysaccharides epitopes. Crystal structure of mAb 023.102 bound to RGP (PDB: 4HIJ) [32], shown in cartoon (A) and surface view (B). The model of mAb 1A6 bound to ManNAc-FucNAc-GalNAc, shown in cartoon (C) and surface view (D). The model of 1A6 fab was built using 023.102 crystal structure as the template (PDB: 4HIJ) [32] by MOE v2018.0101 (Chemical Computing Group). The fragment of serotype 4 polysaccharide (ManNAc-FucNAc-GalNAc) [33, 34] was built in builder and dock with the CDRs of 1A6 fab model (MOE v2018.0101). Cyan, heavy chains; green, light chains; magenta, RGP; dark yellow, ManNAc-FucNAc-GalNAc. The figure was generated by PyMol 1.7.0.5 (SchrĂśdinger). (PDF 2179â kb)

Published
2018
Full Text
View/download PDF

12. Can We Make Small Molecules Lean? Optimization of a Highly Lipophilic TarO Inhibitor

Author

Mandal, Mihirbaran, primary, Tan, Zheng, additional, Madsen-Duggan, Christina, additional, Buevich, Alexei V., additional, Caldwell, John P., additional, Dejesus, Reynalda, additional, Flattery, Amy, additional, Garlisi, Charles G., additional, Gill, Charles, additional, Ha, Sookhee Nicole, additional, Ho, Ginny, additional, Koseoglu, Sandra, additional, Labroli, Marc, additional, Basu, Kallol, additional, Lee, Sang Ho, additional, Liang, Lianzhu, additional, Liu, Jenny, additional, Mayhood, Todd, additional, McGuinness, Debra, additional, McLaren, David G., additional, Wen, Xiujuan, additional, Parmee, Emma, additional, Rindgen, Diane, additional, Roemer, Terry, additional, Sheth, Payal, additional, Tawa, Paul, additional, Tata, James, additional, Yang, Christine, additional, Yang, Shu-Wei, additional, Xiao, Li, additional, Wang, Hao, additional, Tan, Christopher, additional, Tang, Haifeng, additional, Walsh, Paul, additional, Walsh, Erika, additional, Wu, Jin, additional, and Su, Jing, additional

Published
2017
Full Text
View/download PDF

14. Isolation, structure elucidation and antibacterial activity of a new tetramic acid, ascosetin

Author

Ondeyka, John G, primary, Smith, Scott K, additional, Zink, Deborah L, additional, Vicente, Francisca, additional, Basilio, Angela, additional, Bills, Gerald F, additional, Polishook, Jon D, additional, Garlisi, Charles, additional, Mcguinness, Debra, additional, Smith, Elizabeth, additional, Qiu, Hongchen, additional, Gill, Charles J, additional, Donald, Robert GK, additional, Phillips, John W, additional, Goetz, Michael A, additional, and Singh, Sheo B, additional

Published
2014
Full Text
View/download PDF

17. Characterizing Cannabinoid CB2 Receptor Ligands Using DiscoveRx PathHunter™ β-Arrestin Assay.

Author

McGuinness, Debra, Malikzay, Asra, Visconti, Ricahrd, Lin, Karen, Bayne, Marvin, Monsma, Frederick, and Lunn, Charles A.

Subjects
CANNABINOIDS, CELL receptors, LIGANDS (Biochemistry), BIOCHEMISTRY, RADIOLIGAND assay, CELL suspensions
Abstract

The authors have characterized a set of cannabinoid CB[sub2] receptor ligands, including triaryl bis sulfone inverse agonists, in a cell-based receptor/β-arrestin interaction assay (DiscoveRx PathHunter™). The results were compared with results using a competitive ligand binding assay, and with effects on forskolin-stimulated cAMP levels (PerkinElmer LANCE™). The authors show good correlation between the 3 assay systems tested, with the β-arrestin protein complementation assay exhibiting a more robust signal than the cAMP assay for cannabinoid CB2 agonists. Further assay validation shows that DiscoveRx PathHunter™ HEK293 CB2 β-arrestin assay can be carried out from cryopreserved cell suspensions, eliminating variations caused by the need for multiple cell pools during live cell screening campaigns. These results, and the authors' results evaluating a test set of random library compounds, validate the use of ligand-induced interaction between the human cannabinoid CB2 receptor and β-arrestin as an appropriate and valuable screening platform for compounds specific for the cannabinoid CB[sub2] receptor. [ABSTRACT FROM AUTHOR]

Published
2009
Full Text
View/download PDF

18. Screening for Antiviral Inhibitors of the HIV Integrase-LEDGF/p75 Interaction Using the AlphaScreen™ Luminescent Proximity Assay.

Author

Yan Hou, McGuinness, Debra E., Prongay, Andrew J., Feld, Boris, Ingravallo, Paul, Ogert, Robert A., Lunn, Charles A., and Howe, John A.

Subjects
HIV, AIDS, AIDS treatment, PROTEIN-protein interactions, ANTIVIRAL agents
Abstract

Small-molecule inhibitors of HIV integrase (HIV IN) have emerged as a promising new class of antivirals for the treatment of HIV/AIDS. The compounds currently approved or in clinical development specifically target HIV DNA integration and were identified using strand-transfer assays targeting the HIV IN/viral DNA complex. The authors have developed a second biochemical assay for identification of HIV integrase inhibitors, targeting the interaction between HIV IN and the cellular cofactor LEDGF/p75. They developed a luminescent proximity assay (AlphaScreen™) designed to measure the association of the 80-amino-acid integrase binding domain of LEDGF/p75 with the 163-amino-acid catalytic core domain of HIV IN. This assay proved to be quite robust (with a Z' factor of 0.84 in screening libraries arrayed as orthogonal mixtures) and successfully identified several compounds specific for this protein-protein interaction. [ABSTRACT FROM AUTHOR]

Published
2008
Full Text
View/download PDF

19. Characterizing Cannabinoid CB2Receptor Ligands Using DiscoveRx PathHunter™ β-Arrestin Assay

Author

McGuinness, Debra, Malikzay, Asra, Visconti, Richard, Lin, Karen, Bayne, Marvin, Monsma, Frederick, and Lunn, Charles A.

Abstract

The authors have characterized a set of cannabinoid CB2receptor ligands, including triaryl bis sulfone inverse agonists, in a cell-based receptor/β-arrestin interaction assay (DiscoveRx PathHunter™). The results were compared with results using a competitive ligand binding assay, and with effects on forskolin-stimulated cAMP levels (PerkinElmer LANCE™). The authors show good correlation between the 3 assay systems tested, with the β-arrestin protein complementation assay exhibiting a more robust signal than the cAMP assay for cannabinoid CB2agonists. Further assay validation shows that DiscoveRx PathHunter™ HEK293 CB2β-arrestin assay can be carried out from cryopreserved cell suspensions, eliminating variations caused by the need for multiple cell pools during live cell screening campaigns. These results, and the authors’ results evaluating a test set of random library compounds, validate the use of ligand-induced interaction between the human cannabinoid CB2receptor and β-arrestin as an appropriate and valuable screening platform for compounds specific for the cannabinoid CB2receptor.

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results