352 results on '"McCulloch CA"'
Search Results
2. Development of a computer program for teaching periodontal diagnosis based on clinical epidemiological principles
- Author
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Fung, K, primary, Ellen, RP, additional, and McCulloch, CA, additional
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- 1995
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3. Can evidence‐based dental health care assure quality?
- Author
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McCulloch, CA, primary
- Published
- 1994
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4. Long‐term retention after self‐instructional methods
- Author
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Puskas, JC, primary, Lewis, DW, additional, and McCulloch, CA, additional
- Published
- 1992
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5. Dexamethasone recruitment of self-renewing osteoprogenitor cells in chick bone marrow stromal cell cultures
- Author
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Kamalia, N, primary, McCulloch, CA, additional, Tenebaum, HC, additional, and Limeback, H, additional
- Published
- 1992
- Full Text
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6. Osteogenic progenitor cells in rat bone marrow stromal populations exhibit self-renewal in culture
- Author
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McCulloch, CA, primary, Strugurescu, M, additional, Hughes, F, additional, Melcher, AH, additional, and Aubin, JE, additional
- Published
- 1991
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7. Comparison of self‐instruction methods for teaching diagnostic testing
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Puskas, JC, primary, Fung, K, additional, Anderson, JD, additional, Birek, P, additional, Dempster, L, additional, Heft, MW, additional, Torneck, C, additional, Lewis, DW, additional, and McCulloch, CA, additional
- Published
- 1991
- Full Text
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8. Bone sialoprotein does not interact with pro-gelatinase A (MMP-2) or mediate MMP-2 activation.
- Author
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Hwang Q, Cheifetz S, Overall CM, McCulloch CA, Sodek J, Hwang, Queena, Cheifetz, Sela, Overall, Christopher M, McCulloch, Christopher A, and Sodek, Jaro
- Abstract
Background: A recent model for activation of the zymogen form of matrix metalloproteinase 2 (MMP-2, also known as gelatinase A) has suggested that interactions between the SIBLING protein bone sialoprotein (BSP) and MMP-2 leads to conformational change in MMP-2 that initiates the conversion of the pro-enzyme into a catalytically active form. This model is particularly relevant to cancer cell metastasis to bone since BSP, bound to the alphavbeta3 integrin through its arginine-glycine-aspartic acid motif, could recruit MMP-2 to the cell surface.Methods: We critically assessed the relationship between BSP and proMMP-2 and its activation using various forms of recombinant and purified BSP and MMP-2. Gelatinase and collagenase assays, fluorescence binding assays, real-time PCR, cell culture and pull-down assays were employed to test the model.Results: Studies with a fluorogenic substrate for MMP-2 showed no activation of proMMP-2 by BSP. Binding and pull-down assays demonstrated no interaction between MMP-2 and BSP. While BSP-mediated invasiveness has been shown to depend on its integrin-binding RGD sequence, analysis of proMMP-2 activation and the level of membrane type 1 (MT1)-MMP in cells grown on a BSP substratum showed that the BSP-alphavbeta3 integrin interaction does not induce the expression of MT1-MMP.Conclusion: These studies do not support a role for BSP in promoting metastasis through interactions with pro-MMP-2. [ABSTRACT FROM AUTHOR]- Published
- 2009
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9. Critical Factors Affecting Outcomes of Endodontic Microsurgery: A Retrospective Japanese Study.
- Author
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Yamada M, Kasahara N, Matsunaga S, Fujii R, Miyayoshi N, Sekiya S, Ding I, and McCulloch CA
- Abstract
The critically important preoperative and intraoperative factors that affect the success of endodontic microsurgery (EMS) in Japanese patients are not defined. We conducted a retrospective study that analyzed treatment outcomes for 46 teeth in 46 Japanese patients. Treatment was provided between March 2013 and March 2015. All patients were evaluated after one year, the shortest time period over which treatment outcomes after apicoectomy could be evaluated and in which there were complete records for the recruited patient population. Healing was assessed on the basis of clinical symptoms and radiographs. With the use of a binary logistic regression model to quantify success, we estimated the effects of patient age, sex, dental arch, lesion size, lesion type, preoperative root canal treatment, the presence or absence of a post core, and the presence or absence of an isthmus on the surgically prepared dentine surface. The overall success for EMS was 93.5% after one year; failures comprised 6.5%. Successful outcomes were higher ( p = 0.04) for maxillary teeth than for mandibular teeth. Success was higher ( p = 0.019) for patients who received root canal instrumentation prior to EMS. Age, sex, lesion size, lesion type, the presence or absence of a post core, and the presence or absence of a root canal isthmus had no effect ( p > 0.2) on success. We conclude that the percentage of successful outcomes after EMS treatment for Japanese patients presenting with periapical periodontitis is very high after one year and that success is influenced strongly by the dental arch and preoperative root canal instrumentation.
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- 2024
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10. The DIAPH3 linker specifies a β-actin network that maintains RhoA and Myosin-II at the cytokinetic furrow.
- Author
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Shah R, Panagiotou TC, Cole GB, Moraes TF, Lavoie BD, McCulloch CA, and Wilde A
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- Humans, HeLa Cells, Animals, Protein Isoforms metabolism, Protein Isoforms genetics, rhoA GTP-Binding Protein metabolism, rhoA GTP-Binding Protein genetics, Cytokinesis, Formins metabolism, Formins genetics, Actins metabolism, Myosin Type II metabolism, Adaptor Proteins, Signal Transducing metabolism, Adaptor Proteins, Signal Transducing genetics
- Abstract
Cytokinesis is the final step of the cell division cycle that leads to the formation of two new cells. Successful cytokinesis requires significant remodelling of the plasma membrane by spatially distinct β- and γ-actin networks. These networks are generated by the formin family of actin nucleators, DIAPH3 and DIAPH1 respectively. Here we show that β- and γ-actin perform specialized and non-redundant roles in cytokinesis and cannot substitute for one another. Expression of hybrid DIAPH1 and DIAPH3 proteins with altered actin isoform specificity relocalized cytokinetic actin isoform networks within the cell, causing cytokinetic failure. Consistent with this we show that β-actin networks, but not γ-actin networks, are required for the maintenance of non-muscle myosin II and RhoA at the cytokinetic furrow. These data suggest that independent and spatially distinct actin isoform networks form scaffolds of unique interactors that facilitate localized biochemical activities to ensure successful cell division., (© 2024. The Author(s).)
- Published
- 2024
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11. TRPV4 mediates IL-1-induced Ca 2+ signaling, ERK activation and MMP expression.
- Author
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Wang Q, Ji C, Ali A, Ding I, Wang Y, and McCulloch CA
- Subjects
- Animals, Humans, Mice, Calcium metabolism, MAP Kinase Signaling System, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases metabolism, Interleukin-1 metabolism, Interleukin-1 pharmacology, Matrix Metalloproteinase 1 metabolism, Matrix Metalloproteinase 1 genetics, Interleukin-1beta metabolism, Interleukin-1beta pharmacology, TRPV Cation Channels metabolism, TRPV Cation Channels genetics, Fibroblasts metabolism, Calcium Signaling, Gingiva metabolism, Gingiva cytology
- Abstract
Ca
2+ permeation through TRPV4 in fibroblasts is associated with pathological matrix degradation. In human gingival fibroblasts, IL-1β binding to its signaling receptor (IL-1R1) induces activation of extracellular regulated kinase (ERK) and MMP1 expression, processes that require Ca2+ flux across the plasma membrane. It is not known how IL-1R1, which does not conduct Ca2+ , generates Ca2+ signals in response to IL-1. We examined whether TRPV4 mediates the Ca2+ fluxes required for ERK signaling in IL-1 stimulated gingival fibroblasts. TRPV4 was immunostained in fibroblasts of human gingival connective tissue and in focal adhesions of cultured mouse gingival fibroblasts. Human gingival fibroblasts treated with IL-1β showed no change of TRPV4 expression but there was increased MMP1 expression. In mouse, gingival fibroblasts expressing TRPV4, IL-1 strongly increased [Ca2+ ]i . Pre-incubation of cells with IL-1 Receptor Antagonist blocked Ca2+ entry induced by IL-1 or the TRPV4 agonist GSK101. Knockout of TRPV4 or expression of a non-Ca2+ -conducting TRPV4 pore-mutant or pre-incubation with the TRPV4 inhibitor RN1734, blocked IL-1-induced Ca2+ transients and expression of the mouse interstitial collagenase, MMP13. Treatment of mouse gingival fibroblasts with GSK101 phenocopied Ca2+ and ERK responses induced by IL-1; these responses were absent in TRPV4-null cells or cells expressing a non-conducting TRPV4 pore-mutant. Immunostained IL-1R1 localized with TRPV4 in adhesions within cell extensions. While TRPV4 immunoprecipitates analyzed by mass spectrometry showed no association with IL-1R1, TRPV4 associated with Src-related proteins and Src co-immunoprecipitated with TRPV4. Src inhibition reduced IL-1-induced Ca2+ responses. The functional linkage of TRPV4 with IL-1R1 expands its repertoire of innate immune signaling processes by mediating IL-1-driven Ca2+ responses that drive matrix remodeling in fibroblasts. Thus, inhibiting TRPV4 activity may provide a new pharmacological approach for blunting matrix degradation in inflammatory diseases., (© 2024 The Author(s). The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)- Published
- 2024
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12. Impact of TRP Channels on Extracellular Matrix Remodeling: Focus on TRPV4 and Collagen.
- Author
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Wang Q, Ji C, Smith P, and McCulloch CA
- Subjects
- Cell Membrane, Cell-Matrix Junctions, Collagen, Integrins, Humans, Extracellular Matrix, TRPV Cation Channels genetics
- Abstract
Disturbed remodeling of the extracellular matrix (ECM) is frequently observed in several high-prevalence pathologies that include fibrotic diseases of organs such as the heart, lung, periodontium, liver, and the stiffening of the ECM surrounding invasive cancers. In many of these lesions, matrix remodeling mediated by fibroblasts is dysregulated, in part by alterations to the regulatory and effector systems that synthesize and degrade collagen, and by alterations to the functions of the integrin-based adhesions that normally mediate mechanical remodeling of collagen fibrils. Cell-matrix adhesions containing collagen-binding integrins are enriched with regulatory and effector systems that initiate localized remodeling of pericellular collagen fibrils to maintain ECM homeostasis. A large cadre of regulatory molecules is enriched in cell-matrix adhesions that affect ECM remodeling through synthesis, degradation, and contraction of collagen fibrils. One of these regulatory molecules is Transient Receptor Potential Vanilloid-type 4 (TRPV4), a mechanically sensitive, Ca
2+ -permeable plasma membrane channel that regulates collagen remodeling. The gating of Ca2+ across the plasma membrane by TRPV4 and the consequent generation of intracellular Ca2+ signals affect several processes that determine the structural and mechanical properties of collagen-rich ECM. These processes include the synthesis of new collagen fibrils, tractional remodeling by contractile forces, and collagenolysis. While the specific mechanisms by which TRPV4 contributes to matrix remodeling are not well-defined, it is known that TRPV4 is activated by mechanical forces transmitted through collagen adhesion receptors. Here, we consider how TRPV4 expression and function contribute to physiological and pathological collagen remodeling and are associated with collagen adhesions. Over the long-term, an improved understanding of how TRPV4 regulates collagen remodeling could pave the way for new approaches to manage fibrotic lesions.- Published
- 2024
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13. Screening of functionalized collagen membranes with a porcine periodontal regeneration model.
- Author
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Quan BD, Sadeghi R, Ikeda Y, Ganss B, Hamilton DW, McCulloch CA, and Sone ED
- Subjects
- Animals, Swine, Bone Regeneration, Collagen, Dental Cementum, Calcium Phosphates pharmacology, Guided Tissue Regeneration, Periodontal methods, Periodontal Ligament, Insulin-Like Growth Factor I, Alveolar Bone Loss drug therapy
- Abstract
Objectives: Current methods for periodontal regeneration do not promote collagen fiber insertions into new bone and cementum. We used a pig wound model to screen different functionalized collagen membranes in promoting periodontal reattachment to root surfaces., Methods: Treatment groups included (1) control with no membranes, (2) collagen-coated membranes, (3) membranes with insulin-like growth factor-1 (IGF-1), (4) membranes with amelotin, or (5) membranes attached with calcium phosphate cement (CPC), or with CPC combined with IGF-1. Flap procedures were performed on mandibular and maxillary premolars of each pig., Results: Histomorphometric, micro-CT, and clinical measurements obtained at 4 and 12 weeks after surgery showed cementum formation on denuded roots and reformation of alveolar bone, indicating that the pig model can model healing responses in periodontal regeneration. Calcium phosphate cement simplified procedures by eliminating the need for sutures and improved regeneration of alveolar bone (p < 0.05) compared with other treatments. There was a reduction (p < 0.05) of PD only for the IGF group. Large observed variances between treatment groups indicated that a priori power analyses should be conducted to optimize statistical analysis., Conclusions: Pigs can model discrete elements of periodontal healing using collagen-based, functionalized membranes. Screening indicates that membrane anchorage with calcium phosphate cements improve regeneration of alveolar bone., (© 2022 Wiley Periodicals LLC.)
- Published
- 2023
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14. Vimentin-mediated myosin 10 aggregation at tips of cell extensions drives MT1-MMP-dependent collagen degradation in colorectal cancer.
- Author
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Ostrowska-Podhorodecka Z, Ali A, Norouzi M, Ding I, Abbasi S, Arora PD, Wong THF, Magalhaes M, and McCulloch CA
- Subjects
- Humans, Vimentin metabolism, Collagen, Myosins, Matrix Metalloproteinase 14 metabolism, Colorectal Neoplasms
- Abstract
Colorectal cancer (CRC) is a high prevalence adenocarcinoma with progressive increases in metastasis-related mortality, but the mechanisms governing the extracellular matrix (ECM) degradation important for metastasis in CRC are not well-defined. We investigated a functional relationship between vimentin (Vim) and myosin 10 (Myo10), and whether this relationship is associated with cancer progression. We tested the hypothesis that Vim regulates the aggregation of Myo10 at the tips of cell extensions, which increases membrane-type 1 matrix metalloproteinase (MT1-MMP)-associated local collagen proteolysis and ECM degradation. Analysis of CRC samples revealed colocalization of Vim with Myo10 and MT1-MMP in cell extensions adjacent to sites of collagen degradation, suggesting an association with local cell invasion. We analyzed cultured CRC cells and fibroblasts and found that Vim accelerates aggregation of Myo10 at cell tips, which increases the cell extension rate. Vim stabilizes the interaction of Myo10 with MT1-MMP, which in turn increases collagenolysis. Vim depletion reduced the aggregation of Myo10 at the cell extension tips and MT1-MMP-dependent collagenolysis. We propose that Vim interacts with Myo10, which in turn associates with MT1-MMP to facilitate the transport of these molecules to the termini of cell extensions and there enhance cancer invasion of soft connective tissues., (© 2023 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)
- Published
- 2023
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15. TRPV4 regulates β1 integrin-mediated cell-matrix adhesions and collagen remodeling.
- Author
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Ji C, Wang Y, Wang Q, Wang A, Ali A, and McCulloch CA
- Subjects
- Animals, Mice, Cell-Matrix Junctions, Collagen, Focal Adhesions, Integrin beta1, TRPV Cation Channels
- Abstract
Transient Receptor Potential Vanilloid-type 4 (TRPV4) is a mechanosensitive, Ca
2+ -permeable plasma membrane channel that associates with focal adhesions, influences collagen remodeling, and is associated with fibrotic processes through undefined mechanisms. While TRPV4 is known to be activated by mechanical forces transmitted through collagen adhesion receptors containing the β1 integrin, it is not understood whether TRPV4 affects matrix remodeling by altering β1 integrin expression and function. We tested the hypothesis that TRPV4 regulates collagen remodeling through its impact on the β1 integrin in cell-matrix adhesions. In cultured fibroblasts derived from mouse gingival connective tissues, which exhibit very rapid collagen turnover, we found that higher TRPV4 expression is associated with reduced β1 integrin abundance and adhesion to collagen, reduced focal adhesion size and total adhesion area, and reduced alignment and compaction of extracellular fibrillar collagen. The reduction of β1 integrin expression mediated by TRPV4 is associated with the upregulation of miRNAs that target β1 integrin mRNA. Our data suggest a novel mechanism by which TRPV4 modulates collagen remodeling through post-transcriptional downregulation of β1 integrin expression and function., (© 2023 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)- Published
- 2023
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16. DNA hydrogels for bone regeneration.
- Author
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Athanasiadou D, Meshry N, Monteiro NG, Ervolino-Silva AC, Chan RL, McCulloch CA, Okamoto R, and Carneiro KMM
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- Mice, Rats, Animals, X-Ray Microtomography, Bone Regeneration, Biocompatible Materials pharmacology, Biocompatible Materials chemistry, Durapatite pharmacology, Durapatite chemistry, Tissue Engineering, Tissue Scaffolds chemistry, Osteogenesis, Hydrogels chemistry
- Abstract
DNA-based biomaterials have been proposed for tissue engineering approaches due to their predictable assembly into complex morphologies and ease of functionalization. For bone tissue regeneration, the ability to bind Ca
2+ and promote hydroxyapatite (HAP) growth along the DNA backbone combined with their degradation and release of extracellular phosphate, a known promoter of osteogenic differentiation, make DNA-based biomaterials unlike other currently used materials. However, their use as biodegradable scaffolds for bone repair remains scarce. Here, we describe the design and synthesis of DNA hydrogels, gels composed of DNA that swell in water, their interactions in vitro with the osteogenic cell lines MC3T3-E1 and mouse calvarial osteoblast, and their promotion of new bone formation in rat calvarial wounds. We found that DNA hydrogels can be readily synthesized at room temperature, and they promote HAP growth in vitro, as characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. Osteogenic cells remain viable when seeded on DNA hydrogels in vitro, as characterized by fluorescence microscopy. In vivo, DNA hydrogels promote the formation of new bone in rat calvarial critical size defects, as characterized by micro-computed tomography and histology. This study uses DNA hydrogels as a potential therapeutic biomaterial for regenerating lost bone.- Published
- 2023
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17. FLNA- filaminopathy skeletal phenotypes are not due to an osteoblast autonomous loss-of-function.
- Author
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Wade EM, Goodin EA, Wang Y, Morgan T, Callon KE, Watson M, Daniel PB, Cornish J, McCulloch CA, and Robertson SP
- Abstract
Mutations in FLNA , which encodes the cytoskeletal protein FLNA, cause a spectrum of sclerosing skeletal dysplasias. Although many of these genetic variants are recurrent and cluster within the gene, the pathogenic mechanism that underpins the development of these skeletal phenotypes is unknown. To determine if the skeletal dysplasia in FLNA -related conditions is due to a cell-autonomous loss-of-function localising to osteoblasts and/or osteocytes, we utilised mouse models to conditionally remove Flna from this cellular lineage. Flna was conditionally knocked out from mature osteocytes using the Dmp1- promoter driven Cre-recombinase expressing mouse, as well as the committed osteoblast lineage using the Osx- Cre or Col1a1 -Cre expressing lines. We measured skeletal parameters with μCT and histological methods, as well as gene expression in the mineralised skeleton. We found no measureable differences between the conditional Flna knockout mice, and their control littermate counterparts. Moreover, all of the conditional Flna knockout mice, developed and aged normally. From this we concluded that the skeletal dysplasia phenotype associated with pathogenic variants in FLNA is not caused by a cell-autonomous loss-of-function in the osteoblast-osteocyte lineage, adding more evidence to the hypothesis that these phenotypes are due to gain-of-function in FLNA., Competing Interests: The authors have no conflicts of interest to declare., (Crown Copyright © 2023 Published by Elsevier Inc.)
- Published
- 2023
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18. TNF-α regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells.
- Author
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Mezawa M, Tsuruya Y, Yamaguchi A, Yamazaki-Takai M, Kono T, Okada H, McCulloch CA, and Ogata Y
- Subjects
- Basement Membrane, Cell Adhesion physiology, Epithelial Cells, Humans, Laminin, Cell-Matrix Junctions, Tumor Necrosis Factor-alpha
- Abstract
Laminin 5, type 4 collagen, and α6β4 integrin contribute to the formation of hemidesmosomes in the epithelia of periodontal tissues, which is critical for the development and maintenance of the dentogingival junction. As it is not known whether TNF-α alters the composition of the epithelial pericellular matrix, human gingival epithelial cells were cultured in the presence or absence of TNF-α. Treatment with TNF-α accelerated epithelial cell migration and closure of in vitro wounds. These data indicate unexpectedly, that TNF-α promotes the formation of the pericellular matrix around epithelial cells and enhances adhesion of epithelial cells to the underlying matrix, properties which are important for cell migration and the integrity of the dentogingival junction.
- Published
- 2022
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19. Streptococcus mutans Proteases Degrade Dentinal Collagen.
- Author
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Huang B, Stewart CA, McCulloch CA, Santerre JP, Cvitkovitch DG, and Finer Y
- Abstract
Here, we explored the role of S. mutans’s whole cell and discrete fractions in the degradation of type I collagen and dentinal collagen. Type I collagen gels and human demineralized dentin slabs (DS) were incubated in media alone or with one of the following: overnight (O/N) or newly inoculated (NEW) cultures of S. mutans UA159; intracellular proteins, supernatant or bacterial membranes of O/N cultures. Media from all groups were analyzed for protease-mediated release of the collagen-specific imino acid hydroxyproline. Images of type I collagen and DS were analyzed, respectively. Type I collagen degradation was highest for the supernatant (p < 0.05) fractions, followed by intracellular components and O/N cultures. Collagen degradation for DS samples was highest for O/N samples, followed by supernatant, and intracellular components (p < 0.05). There was lower detectable degradation for both type I collagen and DS from NEW culture samples (p < 0.05), and there was no type I collagen or DS degradation detected for bacterial membrane samples. Structural changes to type I collagen gel and dentinal collagen were observed, respectively, following incubation with S. mutans cultures (O/N and NEW), intracellular components, and supernatant. This study demonstrates that intracellular and extracellular proteolytic activities from S. mutans enable this cariogenic bacterium to degrade type I and dentinal collagen in a growth-phase dependent manner, potentially contributing to the progression of dental caries.
- Published
- 2022
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20. DDR1 associates with TRPV4 in cell-matrix adhesions to enable calcium-regulated myosin activity and collagen compaction.
- Author
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Wang AY, Coelho NM, Arora PD, Wang Y, Eymael D, Ji C, Wang Q, Lee W, Xu J, Kapus A, Carneiro KMM, and McCulloch CA
- Subjects
- Calcium, Dietary, Cell-Matrix Junctions metabolism, Collagen metabolism, Myosins metabolism, TRPV Cation Channels genetics, TRPV Cation Channels metabolism, Calcium metabolism, Discoidin Domain Receptor 1 genetics
- Abstract
Tissue fibrosis manifests as excessive deposition of compacted, highly aligned collagen fibrils, which interfere with organ structure and function. Cells in collagen-rich lesions often exhibit marked overexpression of discoidin domain receptor 1 (DDR1), which is linked to increased collagen compaction through the association of DDR1 with the Ca
2+ -dependent nonmuscle myosin IIA (NMIIA). We examined the functional relationship between DDR1 and the transient receptor potential vanilloid type 4 (TRPV4) channel, a Ca2+ -permeable ion channel that is implicated in collagen compaction. Fibroblasts expressing high levels of DDR1 were used to model cells in lesions with collagen compaction. In these cells, the expression of the β1 integrin was deleted to simplify studies of DDR1 function. Compared with DDR1 wild-type cells, high DDR1 expression was associated with increased Ca2+ influx through TRPV4, enrichment of TRPV4 in collagen adhesions, and enhanced contractile activity mediated by NMIIA. At cell adhesion sites to collagen, DDR1 associated with TRPV4, which enhanced DDR1-mediated collagen alignment and compaction. We conclude that DDR1 regulates Ca2+ influx through the TRPV4 channel to promote critical, DDR1-mediated processes that are important in lesions with collagen compaction and alignment., (© 2022 Wiley Periodicals LLC.)- Published
- 2022
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21. Impact of Vimentin on Regulation of Cell Signaling and Matrix Remodeling.
- Author
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Ostrowska-Podhorodecka Z, Ding I, Norouzi M, and McCulloch CA
- Abstract
Vimentin expression contributes to cellular mechanoprotection and is a widely recognized marker of fibroblasts and of epithelial-mesenchymal transition. But it is not understood how vimentin affects signaling that controls cell migration and extracellular matrix (ECM) remodeling. Recent data indicate that vimentin controls collagen deposition and ECM structure by regulating contractile force application to the ECM and through post-transcriptional regulation of ECM related genes. Binding of cells to the ECM promotes the association of vimentin with cytoplasmic domains of adhesion receptors such as integrins. After initial adhesion, cell-generated, myosin-dependent forces and signals that impact vimentin structure can affect cell migration. Post-translational modifications of vimentin determine its adaptor functions, including binding to cell adhesion proteins like paxillin and talin. Accordingly, vimentin regulates the growth, maturation and adhesive strength of integrin-dependent adhesions, which enables cells to tune their attachment to collagen, regulate the formation of cell extensions and control cell migration through connective tissues. Thus, vimentin tunes signaling cascades that regulate cell migration and ECM remodeling. Here we consider how specific properties of vimentin serve to control cell attachment to the underlying ECM and to regulate mesenchymal cell migration and remodeling of the ECM by resident fibroblasts., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Ostrowska-Podhorodecka, Ding, Norouzi and McCulloch.)
- Published
- 2022
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22. Suppression of the fibrotic encapsulation of silicone implants by inhibiting the mechanical activation of pro-fibrotic TGF-β.
- Author
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Noskovicova N, Schuster R, van Putten S, Ezzo M, Koehler A, Boo S, Coelho NM, Griggs D, Ruminski P, McCulloch CA, and Hinz B
- Subjects
- Animals, Fibroblasts, Fibrosis, Foreign-Body Reaction, Mice, Myofibroblasts pathology, Prostheses and Implants, Silicones, Transforming Growth Factor beta
- Abstract
The fibrotic encapsulation of implants involves the mechanical activation of myofibroblasts and of pro-fibrotic transforming growth factor beta 1 (TGF-β1). Here, we show that both softening of the implant surfaces and inhibition of the activation of TGF-β1 reduce the fibrotic encapsulation of subcutaneous silicone implants in mice. Conventionally stiff silicones (elastic modulus, ~2 MPa) coated with a soft silicone layer (elastic modulus, ~2 kPa) reduced collagen deposition as well as myofibroblast activation without affecting the numbers of macrophages and their polarization states. Instead, fibroblasts around stiff implants exhibited enhanced intracellular stress, increased the recruitment of α
v and β1 integrins, and activated TGF-β1 signalling. In vitro, the recruitment of αv integrin to focal adhesions and the activation of β1 integrin and of TGF-β were higher in myofibroblasts grown on latency-associated peptide (LAP)-coated stiff silicones than on soft silicones. Antagonizing αv integrin binding to LAP through the small-molecule inhibitor CWHM-12 suppressed active TGF-β signalling, myofibroblast activation and the fibrotic encapsulation of stiff subcutaneous implants in mice., (© 2021. The Author(s), under exclusive licence to Springer Nature Limited.)- Published
- 2021
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23. Physics and Physiology of Cell Spreading in Two and Three Dimensions.
- Author
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Janmey PA, Hinz B, and McCulloch CA
- Subjects
- Cell Adhesion, Cell Movement, Physics
- Abstract
Cells spread on surfaces and within three-dimensional (3-D) matrixes as they grow, divide, and move. Both chemical and physical signals orchestrate spreading during normal development, wound healing, and pathological states such as fibrosis and tumor growth. Diverse molecular mechanisms drive different forms of cell spreading. This article discusses mechanisms by which cells spread in 2-D and 3-D and illustrates new directions in studies of this aspect of cell function.
- Published
- 2021
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24. Gelsolin is an important mediator of Angiotensin II-induced activation of cardiac fibroblasts and fibrosis.
- Author
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Jana S, Aujla P, Hu M, Kilic T, Zhabyeyev P, McCulloch CA, Oudit GY, and Kassiri Z
- Subjects
- AMP-Activated Protein Kinases antagonists & inhibitors, AMP-Activated Protein Kinases chemistry, AMP-Activated Protein Kinases metabolism, Actins metabolism, Animals, Fibroblasts metabolism, Fibroblasts pathology, Fibrosis metabolism, Gelsolin deficiency, Gelsolin genetics, Homeostasis, Male, Mice, Myocardium metabolism, Myocardium pathology, Myofibroblasts drug effects, Myofibroblasts pathology, Phosphorylation, Ribosomal Protein S6 Kinases, 70-kDa metabolism, Signal Transduction, TOR Serine-Threonine Kinases metabolism, Transforming Growth Factor beta1 metabolism, Angiotensin II pharmacology, Fibroblasts drug effects, Fibrosis pathology, Gelsolin metabolism
- Abstract
Myocardial fibrosis is a characteristic of various cardiomyopathies, and myocardial fibroblasts play a central role in this process. Gelsolin (GSN) is an actin severing and capping protein that regulates actin assembly and may be involved in fibroblast activation. While the role of GSN in mechanical stress-mediated cardiac fibrosis has been explored, its role in myocardial fibrosis in the absence of mechanical stress is not defined. In this study, we investigated the role of GSN in myocardial fibrosis induced by Angiotensin II (Ang II), a profibrotic hormone that is elevated in cardiovascular disease. We utilized mice lacking GSN (Gsn
-/- ) and cultured primary adult cardiac fibroblasts (cFB). In vivo, Ang II infusion in mice resulted in significantly less severe myocardial fibrosis in Gsn-/- compared with Gsn+/+ mice, along with diminished activation of the TGFβ1-Smad2/3 pathway, and reduced expression of cardiac extracellular matrix proteins (collagen, fibronectin, periostin). Moreover, Gsn-deficient hearts exhibited suppressed activity of the AMPK pathway and its downstream effectors, mTOR and P70S6Kinase, which could contribute to the suppressed TGFβ1 activity. In vitro, the Ang II-induced activation of cFBs was reduced in Gsn-deficient fibroblasts evident from decreased expression of αSMA and periostin, diminished actin filament turnover; which also exhibited reduced activity of the AMPK-mTOR pathway, and P70S6K phosphorylation. AMPK inhibition compensated for the loss of GSN, restored the levels of G-actin in Gsn-/- cFBs and promoted activation to myofibroblasts by increasing αSMA and periostin levels. This study reveals a novel role for GSN in mediating myocardial fibrosis by regulating the AMPK-mTOR-P70S6K pathway in cFB activation independent from mechanical stress-induced factors., (© 2021 Federation of American Societies for Experimental Biology.)- Published
- 2021
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25. TRPV4 integrates matrix mechanosensing with Ca 2+ signaling to regulate extracellular matrix remodeling.
- Author
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Ji C and McCulloch CA
- Subjects
- Humans, Calcium metabolism, Cell Adhesion, Extracellular Matrix metabolism, Mechanotransduction, Cellular, TRPV Cation Channels metabolism
- Abstract
In healthy connective tissues, mechanosensors trigger the generation of Ca
2+ signals, which enable cells to maintain the structure of the fibrillar collagen matrix through actomyosin contractile forces. Transient receptor potential vanilloid type 4 (TRPV4) is a mechanosensitive Ca2+ -permeable channel that, when expressed in cell-matrix adhesions of the plasma membrane, regulates extracellular matrix (ECM) remodeling. In high prevalence disorders such as fibrosis and tumor metastasis, dysregulated matrix remodeling is associated with disruptions of Ca2+ homeostasis and TRPV4 function. Here, we consider that ECM polymers transmit cell-activating mechanical signals to TRPV4 in cell adhesions. When activated, TRPV4 regulates fibrillar collagen remodeling, thereby altering the mechanical properties of the ECM. In this review, we integrate functionally connected processes of matrix remodeling to highlight how TRPV4 in cell adhesions and matrix mechanics are reciprocally regulated through Ca2+ signaling., (© 2020 Federation of European Biochemical Societies.)- Published
- 2021
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26. Vimentin regulates the assembly and function of matrix adhesions.
- Author
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Ostrowska-Podhorodecka Z and McCulloch CA
- Subjects
- Cell Adhesion, Collagen, Fibroblasts, Focal Adhesions, Vimentin, Myofibroblasts, Wound Healing
- Abstract
The intermediate filament protein vimentin is a widely used phenotypic marker for identifying cells of the mesenchymal linkage such as fibroblasts and myofibroblasts, but the full repertoire of vimentin's functional attributes has not been fully explored. Here we consider how vimentin, in addition to its contributions to mechanical stabilization of cell structure, also helps to control the assembly of cell adhesions and migration through collagen matrices. While the assembly and function of matrix adhesions are critical for the differentiation of myofibroblasts and many other types of adherent cells, a potential mechanism that explains how vimentin affects the recruitment and abundance of centrally important proteins in cell adhesions has been elusive. Here we review recent data indicating that vimentin plays a central regulatory role in the assembly of focal adhesions which form in response to the attachment to collagen. We show that in particular, vimentin is a key organizer of the β1 integrin adhesive machinery, which affects cell migration through collagen. This review provides a comprehensive picture of the surprisingly broad array of processes and molecules with which vimentin interacts to affect cell function in the context of fibroblast and myofibroblast adhesion and migration on collagen., (© 2021 The Wound Healing Society.)
- Published
- 2021
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27. Vimentin tunes cell migration on collagen by controlling β1 integrin activation and clustering.
- Author
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Ostrowska-Podhorodecka Z, Ding I, Lee W, Tanic J, Abbasi S, Arora PD, Liu RS, Patteson AE, Janmey PA, and McCulloch CA
- Subjects
- Cell Adhesion, Cell Movement, Cluster Analysis, Paxillin genetics, Paxillin metabolism, Vimentin genetics, Vimentin metabolism, Collagen, Integrin beta1 genetics, Integrin beta1 metabolism
- Abstract
Vimentin is a structural protein that is required for mesenchymal cell migration and directly interacts with actin, β1 integrin and paxillin. We examined how these interactions enable vimentin to regulate cell migration on collagen. In fibroblasts, depletion of vimentin increased talin-dependent activation of β1 integrin by more than 2-fold. Loss of vimentin was associated with reduction of β1 integrin clustering by 50% and inhibition of paxillin recruitment to focal adhesions by more than 60%, which was restored by vimentin expression. This reduction of paxillin was associated with 65% lower Cdc42 activation, a 60% reduction of cell extension formation and a greater than 35% decrease in cell migration on collagen. The activation of PAK1, a downstream effector of Cdc42, was required for vimentin phosphorylation and filament maturation. We propose that vimentin tunes cell migration through collagen by acting as an adaptor protein for focal adhesion proteins, thereby regulating β1 integrin activation, resulting in well-organized, mature integrin clusters.This article has an associated First Person interview with the first author of the paper., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2021. Published by The Company of Biologists Ltd.)
- Published
- 2021
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28. Role of the small GTPase activating protein IQGAP1 in collagen phagocytosis.
- Author
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Nakajima K, Arora PD, Plaha A, and McCulloch CA
- Subjects
- Animals, Cell Adhesion genetics, Collagen metabolism, Fibroblasts metabolism, Gingiva metabolism, Gingiva pathology, Humans, Integrin beta1, Mice, Monomeric GTP-Binding Proteins genetics, Protein Binding genetics, Signal Transduction genetics, Collagen genetics, Microfilament Proteins genetics, Phagocytosis genetics, Trans-Activators genetics, cdc42 GTP-Binding Protein genetics, ras GTPase-Activating Proteins genetics
- Abstract
Many adult connective tissues undergo continuous remodeling to maintain matrix homeostasis. Physiological remodeling involves the degradation of collagen fibers by the intracellular cathepsin-dependent phagocytic pathway. We considered that a multidomain, small GTPase activating protein, IQGAP1, which is involved in the generation of cell extensions, is required for collagen phagocytosis, possibly arising from its interactions with cdc42 and the actin-binding protein Flightless I (FliI). We examined the role of IQGAP1 in collagen phagocytosis by human gingival fibroblasts (HGFs) and by IQGAP1+/+ and IQGAP1-/- mouse embryonic fibroblasts. IQGAP1 was strongly expressed by HGFs, localized to vinculin-stained cell adhesions and sites where cell extensions are initiated, and colocalized with FliI. Immunoprecipitation showed that IQGAP1 associated with FliI. HGFs showed 10-fold increases of collagen binding, 6-fold higher internalization, and 3-fold higher β1 integrin activation between 30 and 180 min after incubation with collagen. Compared with IQGAP1+/+ fibroblasts, deletion of IQGAP1 reduced collagen binding (1.4-fold), collagen internalization (3-fold), β1 integrin activation (2-fold), and collagen degradation (1.8-fold). We conclude that IQGAP1 affects collagen remodeling through its regulation of phagocytic degradation pathways, which may involve the interaction of IQGAP1 with FliI., (© 2020 Wiley Periodicals LLC.)
- Published
- 2021
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29. Myofibroblast Adhesome Analysis by Mass Spectrometry.
- Author
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McCulloch CA
- Subjects
- Cell Adhesion, Cells, Cultured, Humans, Mass Spectrometry, Focal Adhesions metabolism, Myofibroblasts metabolism, Proteomics methods
- Abstract
Myofibroblasts form adhesions to their underlying extracellular matrices, which is an essential step in their formation and differentiation. These adhesions comprise protein-rich aggregates of a wide variety of signaling, cytoskeletal, cell adhesion, and matrix proteins that interact with one another to enable bidirectional flow of information between the cell and the surrounding extracellular matrix. The concentrated repertoire of the proteins in matrix adhesions of myofibroblasts (i.e., over 450 different proteins) and their important role in regulating the metabolic activities of myofibroblasts, has motivated in-depth analysis of their protein complement and how this repertoire is influenced by experimental conditions.In this protocol I describe in detail: (1) the method for isolating focal adhesion-associated proteins using matrix ligand-bound magnetite beads; (2) the method for eluting the proteins from the beads and their preparation for mass spectrometry (Fig. 1). I also briefly consider the mass spectrometry methods including the use of isobaric tags to enable multifactorial experiments and the analysis of the identified proteins. I consider the advantages of these approaches, and the challenges and pitfalls that are encountered with these methods.
- Published
- 2021
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30. CD301 mediates fusion in IL-4-driven multinucleated giant cell formation.
- Author
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Brooks PJ, Wang Y, Magalhaes MA, Glogauer M, and McCulloch CA
- Subjects
- Animals, Antibodies, Macrophages, Mice, Asialoglycoproteins, Cell Fusion, Giant Cells, Interleukin-4 genetics, Lectins, C-Type, Membrane Proteins, Monocytes
- Abstract
Multinucleated giant cells (MGCs) are prominent in foreign body granulomas, infectious and inflammatory processes, and auto-immune, neoplastic and genetic disorders, but the molecular determinants that specify the formation and function of these cells are not defined. Here, using tandem mass tag-mass spectrometry, we identified a differentially upregulated protein, C-type lectin domain family 10 member (herein denoted CD301, also known as CLEC10A), that was strongly upregulated in mouse RAW264.7 macrophages and primary murine macrophages undergoing interleukin (IL-4)-induced MGC formation. CD301
+ MGCs were identified in biopsy specimens of human inflammatory lesions. Function-inhibiting CD301 antibodies or CRISPR/Cas9 deletion of the two mouse CD301 genes (Mgl1 and Mgl2) inhibited IL-4-induced binding of N-acetylgalactosamine-coated beads by 4-fold and reduced MGC formation by 2.3-fold ( P <0.05). IL-4-driven fusion and MGC formation were restored by re-expression of CD301 in the knockout cells. We conclude that in monocytes, IL-4 increases CD301 expression, which mediates intercellular adhesion and fusion processes that are required for the formation of MGCs.This article has an associated First Person interview with the first author of the paper., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2020. Published by The Company of Biologists Ltd.)- Published
- 2020
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31. How Tony Melcher advanced our understanding of periodontal biology and regeneration.
- Author
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Brunette DM, Ellen RP, and McCulloch CA
- Subjects
- Biology history, Connective Tissue, History, 20th Century, History, 21st Century, Humans, Periodontal Ligament, Periodontium, Regeneration
- Abstract
Tony Melcher, a highly influential and forward-thinking scientist and teacher, focussed on the origins, behaviour and regulation of cells in periodontal tissues. His recent death in April 2020, has motivated us to highlight his multi-level contributions to research in biology and the dental sciences. Tony was particularly adept at recognizing the inherent instructive power of the periodontium, most notably as a model system for studying the inter-relationships between the structure, development and functions of connective tissues. Further, his mentoring of dozens of students who subsequently went on to develop their own careers in research, and his leadership in promoting collaborations in dental sciences world-wide, engendered important advances in the importance and utility of research relating to oral tissues. Here, we reflect upon his development of a large, multi-disciplinary research enterprise, the MRC Group in Periodontal Physiology at the University of Toronto and brief commentaries of those who worked with him there. We examine his early career development and then go on to consider some of his most highly cited publications and their impact on subsequent research trends., (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2020
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32. The Neutrophil: Constant Defender and First Responder.
- Author
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Fine N, Tasevski N, McCulloch CA, Tenenbaum HC, and Glogauer M
- Subjects
- Animals, Homeostasis, Humans, Immunity, Mucosal, Dysbiosis immunology, Extracellular Traps metabolism, Inflammation immunology, Mucous Membrane immunology, Neutrophils immunology
- Abstract
The role of polymorphonuclear neutrophils (PMNs) in biology is often recognized during pathogenesis associated with PMN hyper- or hypo-functionality in various disease states. However, in the vast majority of cases, PMNs contribute to resilience and tissue homeostasis, with continuous PMN-mediated actions required for the maintenance of health, particularly in mucosal tissues. PMNs are extraordinarily well-adapted to respond to and diminish the damaging effects of a vast repertoire of infectious agents and injurious processes that are encountered throughout life. The commensal biofilm, a symbiotic polymicrobial ecosystem that lines the mucosal surfaces, is the first line of defense against pathogenic strains that might otherwise dominate, and is therefore of critical importance for health. PMNs regularly interact with the commensal flora at the mucosal tissues in health and limit their growth without developing an overt inflammatory reaction to them. These PMNs exhibit what is called a para-inflammatory phenotype, and have reduced inflammatory output. When biofilm growth and makeup are disrupted (i.e., dysbiosis), clinical symptoms associated with acute and chronic inflammatory responses to these changes may include pain, erythema and swelling. However, in most cases, these responses indicate that the immune system is functioning properly to re-establish homeostasis and protect the status quo. Defects in this healthy everyday function occur as a result of PMN subversion by pathological microbial strains, genetic defects or crosstalk with other chronic inflammatory conditions, including cancer and rheumatic disease, and this can provide some avenues for therapeutic targeting of PMN function. In other cases, targeting PMN functions could worsen the disease state. Certain PMN-mediated responses to pathogens, for example Neutrophil Extracellular Traps (NETs), might lead to undesirable symptoms such as pain or swelling and tissue damage/fibrosis. Despite collateral damage, these PMN responses limit pathogen dissemination and more severe damage that would otherwise occur. New data suggests the existence of unique PMN subsets, commonly associated with functional diversification in response to particular inflammatory challenges. PMN-directed therapeutic approaches depend on a greater understanding of this diversity. Here we outline the current understanding of PMNs in health and disease, with an emphasis on the positive manifestations of tissue and organ-protective PMN-mediated inflammation., (Copyright © 2020 Fine, Tasevski, McCulloch, Tenenbaum and Glogauer.)
- Published
- 2020
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33. Cooperative roles of PAK1 and filamin A in regulation of vimentin assembly and cell extension formation.
- Author
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Ding I, Ostrowska-Podhorodecka Z, Lee W, Liu RSC, Carneiro K, Janmey PA, and McCulloch CA
- Subjects
- Animals, Gene Knockdown Techniques, Mice, Phosphorylation, Protein Binding, p21-Activated Kinases genetics, Cell Surface Extensions metabolism, Filamins metabolism, Vimentin metabolism, p21-Activated Kinases metabolism
- Abstract
The formation of extensions in cell migration requires tightly coordinated reorganization of all three cytoskeletal polymers but the mechanisms by which intermediate filament networks interact with actin to generate extensions are not well-defined. We examined interactions of the actin binding protein filamin A (FLNA) with vimentin in extension formation by fibroblasts. Knockdown (KD) of vimentin in fibroblasts reduced the lengths of cell extensions by 50% (p < 0.001). After cell binding to fibronectin, there was a time-dependent increase of phosphorylation of serine 39, 56 and 72 in vimentin, which was associated with vimentin filament assembly. Of the FLNA-interacting kinases that could phosphorylate vimentin, we focused on PAK1, which we found by reciprocal immunoprecipitation associated with FLNA. Enzyme inhibitor studies and siRNA KD demonstrated that PAK1 was required for vimentin phosphorylation and formation of cell extensions. In sedimentation assays, vimentin was exclusively detected in the insoluble pellet fraction of cells expressing FLNA while in FLNA KD cells there was increased vimentin in the supernatants of FLN KD cells. Compared with wild type, FLNA KD cells showed loss of phosphorylation of serine 56 and 72 in vimentin and reduced numbers and lengths of cell extensions by >4-fold. We suggest that the association of PAK1 with FLNA enables vimentin phosphorylation and filament assembly, which are important in the development and stabilization of cell extensions during cell migration., (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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34. Flightless anchors IQGAP1 and R-ras to mediate cell extension formation and matrix remodeling.
- Author
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Arora PD, Nakajima K, Nanda A, Plaha A, Wilde A, Sacks DB, and McCulloch CA
- Subjects
- 3T3 Cells, Animals, Cell Adhesion, Collagen pharmacology, Mice, Models, Biological, Protein Binding drug effects, Protein Domains, cdc42 GTP-Binding Protein metabolism, ras GTPase-Activating Proteins chemistry, Cell Surface Extensions metabolism, Extracellular Matrix metabolism, Microfilament Proteins metabolism, Trans-Activators metabolism, ras GTPase-Activating Proteins metabolism, ras Proteins metabolism
- Abstract
Tractional remodeling of collagen fibrils by fibroblasts requires long cell extensions that mediate fibril alignment. The formation of these cell extensions involves flightless I (FliI), an actin-binding protein that contains a leucine-rich-repeat (LRR), which binds R-ras and may regulate cdc42. We considered that FliI interacts with small GTPases and their regulators to mediate assembly of cell extensions. Mass spectrometry analyses of FliI immunoprecipitates showed abundant Ras GTPase-activating-like protein (IQGAP1), which in immunostained samples colocalized with FliI at cell adhesions. Knockdown of IQGAP1 reduced the numbers of cell extensions and the alignment of collagen fibrils. In experiments using dominant negative mutants, cdc42 activity was required for the formation of short extensions while R-ras was required for the formation of long extensions. Immunoprecipitation of wild-type and mutant constructs showed that IQGAP1 associated with cdc42 and R-ras; this association required the GAP-related domain (1004-1237 aa) of IQGAP1. In cells transfected with FliI mutants, the LRR of FliI, but not its gelsolin-like domains, mediated association with cdc42, R-ras, and IQGAP1. We conclude that FliI interacts with IQGAP1 and co-ordinates with cdc42 and R-ras to control the formation of cell extensions that enable collagen tractional remodeling.
- Published
- 2020
- Full Text
- View/download PDF
35. MRIP Regulates the Myosin IIA Activity and DDR1 Function to Enable Collagen Tractional Remodeling.
- Author
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Coelho NM, Wang A, Petrovic P, Wang Y, Lee W, and McCulloch CA
- Subjects
- Animals, Cattle, Cell Adhesion, Cell Line, Cell Movement, Cell Proliferation, Mice, Knockout, Models, Biological, Protein Stability, Adaptor Proteins, Signal Transducing metabolism, Collagen metabolism, Discoidin Domain Receptor 1 metabolism, Microfilament Proteins metabolism, Nonmuscle Myosin Type IIA metabolism
- Abstract
DDR1 is a collagen adhesion-mechanoreceptor expressed in fibrotic lesions. DDR1 mediates non-muscle myosin IIA (NMIIA)-dependent collagen remodeling. We discovered that the myosin phosphatase Rho-interacting protein (MRIP), is enriched in DDR1-NMIIA adhesions on collagen. MRIP regulates RhoA- and myosin phosphatase-dependent myosin activity. We hypothesized that MRIP regulates DDR1-NMIIA interactions to enable cell migration and collagen tractional remodeling. After deletion of MRIP in β1-integrin null cells expressing DDR1, in vitro wound closure, collagen realignment, and contraction were reduced. Cells expressing DDR1 and MRIP formed larger and more abundant DDR1 clusters on collagen than cells cultured on fibronectin or cells expressing DDR1 but null for MRIP or cells expressing a non-activating DDR1 mutant. Deletion of MRIP reduced DDR1 autophosphorylation and blocked myosin light chain-dependent contraction. Deletion of MRIP did not disrupt the association of DDR1 with NMIIA. We conclude that MRIP regulates NMIIA-dependent DDR1 cluster growth and activation. Accordingly, MRIP may provide a novel drug target for dysfunctional DDR1-related collagen tractional remodeling in fibrosis.
- Published
- 2020
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- View/download PDF
36. The Leucine-Rich Repeat Region of CARMIL1 Regulates IL-1-Mediated ERK Activation, MMP Expression, and Collagen Degradation.
- Author
-
Wang Q, Notay K, Downey GP, and McCulloch CA
- Subjects
- Adult, Amino Acid Sequence, Animals, Cattle, Cell-Penetrating Peptides chemistry, Cell-Penetrating Peptides pharmacology, Enzyme Activation, Female, Humans, Interleukin-1 Receptor-Associated Kinases metabolism, Male, Middle Aged, Models, Biological, Phosphorylation, Protein Binding, Protein Domains, Receptors, Interleukin-1 Type I metabolism, Repetitive Sequences, Amino Acid, Signal Transduction, Structure-Activity Relationship, Collagen metabolism, Extracellular Signal-Regulated MAP Kinases metabolism, Interleukin-1 metabolism, Leucine chemistry, Matrix Metalloproteinase 3 metabolism, Microfilament Proteins chemistry, Microfilament Proteins metabolism, Proteolysis
- Abstract
CARMILs are large, multidomain, membrane-associated proteins that regulate actin assembly and Rho-family GTPases, but their role in inflammatory signaling is not defined. Tandem mass tag mass spectrometry indicated that, in fibroblasts, CARMIL1 associates with interleukin (IL)-1 signaling molecules. Immunoprecipitation of cells transfected with CARMIL1 mutants showed that the leucine-rich repeat (LRR) region of CARMIL1 associates with IL-1 receptor type 1 (IL-1R1) and IL-1 receptor-associated kinase (IRAK). Knockout of CARMIL1 by CRISPR-Cas9 reduced IL-1-induced ERK activation by 72% and MMP3 expression by 40%. Compared with CARMIL1 wild-type (WT), cells expressing mutant CARMIL1 lacking its LRR domain exhibited 45% lower ERK activation and 40% lower MMP3 expression. In fibroblasts transduced with a cell-permeable, TAT CARMIL1 peptide that competed with IL-1R1 and IRAK binding to the LRR of CARMIL1, collagen degradation was reduced by 43%. As the LRR of CARMIL1 evidently regulates IL-1 signaling, CARMIL1 could become a target for anti-inflammatory drug development., Competing Interests: Declaration of Interests The authors declare no competing interests., (Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2020
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37. The scaffold-protein IQGAP1 enhances and spatially restricts the actin-nucleating activity of Diaphanous-related formin 1 (DIAPH1).
- Author
-
Chen A, Arora PD, Lai CC, Copeland JW, Moraes TF, McCulloch CA, Lavoie BD, and Wilde A
- Subjects
- Actin Cytoskeleton metabolism, Cell Line, Tumor, Formins antagonists & inhibitors, Formins genetics, Humans, Microfilament Proteins antagonists & inhibitors, Microfilament Proteins genetics, Microfilament Proteins metabolism, Protein Binding, RNA Interference, RNA, Small Interfering metabolism, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins isolation & purification, Trans-Activators antagonists & inhibitors, Trans-Activators genetics, Trans-Activators metabolism, ras GTPase-Activating Proteins antagonists & inhibitors, ras GTPase-Activating Proteins genetics, rhoA GTP-Binding Protein metabolism, Actins metabolism, Formins metabolism, ras GTPase-Activating Proteins metabolism
- Abstract
The actin cytoskeleton is a dynamic array of filaments that undergoes rapid remodeling to drive many cellular processes. An essential feature of filament remodeling is the spatio-temporal regulation of actin filament nucleation. One family of actin filament nucleators, the Diaphanous-related formins, is activated by the binding of small G-proteins such as RhoA. However, RhoA only partially activates formins, suggesting that additional factors are required to fully activate the formin. Here we identify one such factor, IQ motif containing GTPase activating protein-1 (IQGAP1), which enhances RhoA-mediated activation of the Diaphanous-related formin (DIAPH1) and targets DIAPH1 to the plasma membrane. We find that the inhibitory intramolecular interaction within DIAPH1 is disrupted by the sequential binding of RhoA and IQGAP1. Binding of RhoA and IQGAP1 robustly stimulates DIAPH1-mediated actin filament nucleation in vitro In contrast, the actin capping protein Flightless-I, in conjunction with RhoA, only weakly stimulates DIAPH1 activity. IQGAP1, but not Flightless-I, is required to recruit DIAPH1 to the plasma membrane where actin filaments are generated. These results indicate that IQGAP1 enhances RhoA-mediated activation of DIAPH1 in vivo Collectively these data support a model where the combined action of RhoA and an enhancer ensures the spatio-temporal regulation of actin nucleation to stimulate robust and localized actin filament production in vivo ., (© 2020 Chen et al.)
- Published
- 2020
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38. Focal adhesion kinase regulates tractional collagen remodeling, matrix metalloproteinase expression, and collagen structure, which in turn affects matrix-induced signaling.
- Author
-
Rajshankar D, Wang B, Worndl E, Menezes S, Wang Y, and McCulloch CA
- Subjects
- Animals, Cell Adhesion physiology, Cells, Cultured, Collagen Type I metabolism, Fibroblasts metabolism, Focal Adhesion Protein-Tyrosine Kinases genetics, Mice, Signal Transduction genetics, Signal Transduction physiology, Collagen metabolism, Extracellular Signal-Regulated MAP Kinases metabolism, Focal Adhesion Protein-Tyrosine Kinases metabolism, Matrix Metalloproteinases metabolism
- Abstract
Focal adhesion kinase (FAK) is critical for collagen expression but its regulation of collagen remodeling is not defined. We examined the role of FAK in the degradation and reorganization of fibrillar collagen. Compared with wild-type (WT) mouse embryonic fibroblasts, FAK null (FAK
-/- ) fibroblasts generated twofold (p < .0001) higher levels of ¾ collagen I fragment and expressed up to fivefold more membrane-type matrix metalloproteinase (MMP). When plated on stiff collagen substrates, compared with WT, FAK-/- cells were smaller (threefold reduced cell surface area; p < .0001) and produced fivefold fewer cell extensions (p < .0001) that were 40% shorter (p < .001). When cultured on soft collagen gels (stiffness of ~100 Pa) for 6-48 hr, cell spreading and cell extension formation were reduced by greater than twofold (p < .05 and p < .0001, respectively) while collagen compaction and alignment were reduced by approximately 30% (p < .0001) in FAK-/- cells. Similar results were found after treatment with PF573228, a FAK inhibitor. Reconstitution of FAK-/- cells with FAK mutants showed that compared with WT, cell extension formation was reduced twofold (p < .0001) in the absence of the kinase domain and sixfold (p < .0001) with a Y397F mutant. Enhanced collagen degradation was exhibited by the mutants (~threefold increase; p < .0001 of ¾ collagen fragments without kinase domain or Y397F mutant; p < .01). Compared with FAK+/+ cells, matrices produced by FAK-/- cells generated higher levels of β1 integrin activation (p < 0.05), extracellular-signal-regulated kinase (ERK) phosphorylation, and production of ¾ collagen I fragment by human gingival fibroblasts. Collectively these data indicate that (a) the kinase activity of FAK enhances collagen remodeling by tractional forces but inhibits collagen degradation by MMPs; (b) FAK influences the biological activity of fibroblast-secreted extracellular matrices, which in turn impacts β1 integrin and ERK signaling, and collagen degradation., (© 2019 Wiley Periodicals, Inc.)- Published
- 2020
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39. Loss of Vimentin Enhances Cell Motility through Small Confining Spaces.
- Author
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Patteson AE, Pogoda K, Byfield FJ, Mandal K, Ostrowska-Podhorodecka Z, Charrier EE, Galie PA, Deptuła P, Bucki R, McCulloch CA, and Janmey PA
- Subjects
- Animals, Biomechanical Phenomena, Capillaries drug effects, Collagen pharmacology, Cytoskeleton metabolism, Hydrogels pharmacology, Mice, Myosin Type II metabolism, NIH 3T3 Cells, Vimentin metabolism, Cell Movement, Vimentin deficiency
- Abstract
The migration of cells through constricting spaces or along fibrous tracks in tissues is important for many biological processes and depends on the mechanical properties of a cytoskeleton made up of three different filaments: F-actin, microtubules, and intermediate filaments. The signaling pathways and cytoskeletal structures that control cell motility on 2D are often very different from those that control motility in 3D. Previous studies have shown that intermediate filaments can promote actin-driven protrusions at the cell edge, but have little effect on overall motility of cells on flat surfaces. They are however important for cells to maintain resistance to repeated compressive stresses that are expected to occur in vivo. Using mouse embryonic fibroblasts derived from wild-type and vimentin-null mice, it is found that loss of vimentin increases motility in 3D microchannels even though on flat surfaces it has the opposite effect. Atomic force microscopy and traction force microscopy experiments reveal that vimentin enhances perinuclear cell stiffness while maintaining the same level of acto-myosin contractility in cells. A minimal model in which a perinuclear vimentin cage constricts along with the nucleus during motility through confining spaces, providing mechanical resistance against large strains that could damage the structural integrity of cells, is proposed., (© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2019
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40. IL-1β enhances cell adhesion through laminin 5 and β4 integrin in gingival epithelial cells.
- Author
-
Mezawa M, Tsuruya Y, Yamazaki-Takai M, Takai H, Nakayama Y, McCulloch CA, and Ogata Y
- Subjects
- Cell Adhesion, Cell Adhesion Molecules, Interleukin-1beta, Kalinin, Epithelial Cells, Integrin beta4
- Abstract
The junctional epithelium and dental enamel adhere because of hemidesmosomes containing laminin 5 and α6β4 integrin, which are important adhesion molecules in the internal basal lamina. Interleukin (IL)-1 is important in the pathogenesis of periodontal disease. IL-1β induces bone resorption by activating osteoclasts; however, its effects on adhesion of epithelial cells remain to be clarified. Laminin β3, β4 integrin, and focal adhesion kinase mRNA levels were higher after 1 h and 3 h of stimulation with IL-1β (1 ng/mL), and IL-1β, type I α1, and type IV α1 collagen mRNA levels were higher after 1 h and lower after 3 h of stimulation with IL-1β. After IL-1β stimulation, colocalization of laminin 5 and β4 integrin was increased after 1 h, colocalization of β4 integrin and plectin was increased after 1 h and decreased after 3 h, and colocalization of β4 integrin and type IV collagen was decreased after 3 h. Wound healing assays showed that IL-1β treatment (3 h) delayed wound healing. These results suggest that IL-1β enhances cell adhesion by altering localization of epithelial adhesion molecules.
- Published
- 2019
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- View/download PDF
41. Discoidin domain receptor 1 interactions with myosin motors contribute to collagen remodeling and tissue fibrosis.
- Author
-
Coelho NM, Wang A, and McCulloch CA
- Subjects
- Animals, Cell Adhesion genetics, Collagen metabolism, Discoidin Domain Receptor 1 genetics, Extracellular Matrix metabolism, Humans, Myosins metabolism, Platelet Glycoprotein GPIb-IX Complex, Signal Transduction, Collagen chemistry, Discoidin Domain Receptor 1 chemistry, Discoidin Domain Receptor 1 metabolism, Fibrosis metabolism, Myosins chemistry, Protein Interaction Domains and Motifs
- Abstract
Discoidin Domain Receptor (DDR) genes and their homologues have been identified in sponges, worms and flies. These genes code for proteins that are implicated in cell adhesion to matrix proteins. DDRs are now recognized as playing central regulatory roles in several high prevalence human diseases, including invasive cancers, atherosclerosis, and organ fibrosis. While the mechanisms by which DDRs contribute to these diseases are just now being delineated, one of the common themes involves cell adhesion to collagen and the assembly and organization of collagen fibers in the extracellular matrix. In mammals, the multi-functional roles of DDRs in promoting cell adhesion to collagen fibers and in mediating collagen-dependent signaling, suggest that DDRs contribute to multiple pathways of extracellular matrix remodeling, which are centrally important processes in health and disease. In this review we consider that interactions of the cytoplasmic domains of DDR1 with cytoskeletal motor proteins may contribute to matrix remodeling by promoting collagen fiber alignment and compaction. Poorly controlled collagen remodeling with excessive compaction of matrix proteins is a hallmark of fibrotic lesions in many organs and tissues that are affected by infectious, traumatic or chemical-mediated injury. An improved understanding of the mechanisms by which DDRs mediate collagen remodeling and collagen-dependent signaling could suggest new drug targets for treatment of fibrotic diseases., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
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42. Adseverin modulates morphology and invasive function of MCF7 cells.
- Author
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Tanic J, Wang Y, Lee W, Coelho NM, Glogauer M, and McCulloch CA
- Subjects
- Actins metabolism, Breast Neoplasms metabolism, CRISPR-Cas Systems, Cell Movement, Collagen metabolism, Fibronectins metabolism, Gelsolin genetics, Humans, Phagocytosis, Gelsolin metabolism, MCF-7 Cells metabolism
- Abstract
Adseverin (Ads) is a Ca
2+ -dependent actin-capping and severing protein that is highly expressed in gastric, prostate and bladder cancer cells. Currently it is unknown whether Ads contributes to the subcortical actin remodeling associated with the formation of cell extensions and matrix invasion in cancer. We compared cell extension formation and matrix degradation in Ads wildtype and Ads-null MCF7 breast cancer cells generated by CRISPR/Cas9. Compared with wildtype, Ads-null cells plated on fibronectin or collagen exhibited a more circular morphology with shorter cell extensions (37% reduction on fibronectin; p < 0.001). Reconstitution of Ads in Ads-null cells restored the formation of cell extensions (p < 0.05). While cell migration on two-dimensional matrices was unchanged by Ads deletion, the formation of cell extensions across Transwell membranes was reduced (~40% reduction, p < 0.05). When plated on fibrillar collagen, compared with wildtype, Ads-null cells showed reduced expression of MT1-MMP, collagen degradation (p < 0.05) and phagocytosis of collagen-coated beads (25% reduction; p = 0.001). We conclude that Ads is involved in the formation of cell extensions and collagen degradation in MCF7 cells, which may in turn affect matrix invasion and metastasis., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
43. An Overview of the Derivation and Function of Multinucleated Giant Cells and Their Role in Pathologic Processes.
- Author
-
Brooks PJ, Glogauer M, and McCulloch CA
- Subjects
- Bone Cysts, Aneurysmal pathology, Bone Neoplasms pathology, Cell Adhesion Molecules physiology, Cell Differentiation physiology, Cell Fusion, Cherubism pathology, Giant Cell Arteritis pathology, Giant Cell Tumor of Bone pathology, Granuloma, Foreign-Body pathology, Humans, Macrophage Activation physiology, Monocytes physiology, Osteosarcoma pathology, Tuberculosis pathology, Giant Cells pathology
- Abstract
Monocyte lineage cells play important roles in health and disease. Their differentiation into macrophages is crucial for a broad array of immunologic processes that regulate inflammation, neoplasia, and infection. In certain pathologic conditions, such as foreign body reactions and peripheral inflammatory lesions, monocytes fuse to form large, multinucleated giant cells (MGCs). Currently, our knowledge of the fusion mechanisms of monocytes and the regulation of MGC formation and function in discrete pathologies is limited. Herein, we consider the types and function of MGCs in disease and assess the mechanisms by which monocyte fusion contributes to the formation of MGCs. An improved understanding of the cellular origins and metabolic functions of MGCs will facilitate their identification and ultimately the treatment of diseases and disorders that involve MGCs., (Copyright © 2019 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2019
- Full Text
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44. Mechanical regulation of myofibroblast phenoconversion and collagen contraction.
- Author
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Hinz B, McCulloch CA, and Coelho NM
- Subjects
- Animals, Extracellular Matrix metabolism, Extracellular Matrix pathology, Fibrosis metabolism, Fibrosis pathology, Humans, Phenotype, Wound Healing physiology, Collagen metabolism, Myofibroblasts metabolism, Myofibroblasts pathology
- Abstract
Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conversion into highly contractile myofibroblasts by developing muscle-like features, including formation of contractile actin-myosin bundles. The phenotype and function of fibroblasts and myofibroblasts are mechanically regulated by matrix stiffness using a feedback control system that is integrated with the progress of tissue remodelling. The actomyosin contraction machinery and cell-matrix adhesion receptors are critical elements that are needed for mechanosensing by fibroblasts and the translation of mechanical signals into biological responses. Here, we focus on mechanical and chemical regulation of collagen contraction by fibroblasts and the involvement of these factors in their phenotypic conversion to myofibroblasts., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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45. Author Correction: Dynamic fibroblast contractions attract remote macrophages in fibrillar collagen matrix.
- Author
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Pakshir P, Alizadehgiashi M, Wong B, Coelho NM, Chen X, Gong Z, Shenoy VB, McCulloch CA, and Hinz B
- Abstract
The original version of this Article contained an error in the spelling of the author Christopher A. McCulloch, which was incorrectly given as Christopher McCulloch. This has now been corrected in both the PDF and HTML versions of the Article.
- Published
- 2019
- Full Text
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46. Role of Fibroblast Populations in Periodontal Wound Healing and Tissue Remodeling.
- Author
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Smith PC, Martínez C, Martínez J, and McCulloch CA
- Abstract
After injury to periodontal tissues, a sequentially phased healing response is initiated that enables wound closure and partial restoration of tissue structure and function. Wound closure in periodontal tissues involves the tightly regulated coordination of resident cells in epithelial and connective tissue compartments. Multiple cell populations in these compartments synergize their metabolic activities to reestablish a mucosal seal that involves the underlying periodontal connective tissues and the attachment of these tissues to the tooth surface. The formation of an impermeable seal around the circumference of the tooth is of particular significance in oral health since colonization of tooth surfaces by pathogenic biofilms promotes inflammation, which can contribute to periodontal tissue degradation and tooth loss. The reformation of periodontal tissue structures in the healing response centrally involves fibroblasts, which synthesize and organize the collagen fibers that link alveolar bone and gingiva to the cementum covering the tooth root. The synthesis and remodeling of nascent collagen matrices are of fundamental importance for the reestablishment of a functional periodontium and are mediated by diverse, multi-functional fibroblast populations that reside within the connective tissues of gingiva and periodontal ligament. Notably, after gingival wounding, a fibroblast sub-type (myofibroblast) arises, which is centrally involved in collagen synthesis and fibrillar remodeling. While myofibroblasts are not usually seen in healthy, mature connective tissues, their formation is enhanced by wound-healing cytokines. The formation of myofibroblasts is also modulated by the stiffness of the extracellular matrix, which is mechanosensed by resident precursor cells in the gingival connective tissue microenvironment. Here, we consider the cellular origins and the factors that control the differentiation and matrix remodeling functions of periodontal fibroblasts. An improved understanding of the regulation and function of periodontal fibroblasts will be critical for the development of new therapies to optimize the restoration of periodontal structure and function after wounding.
- Published
- 2019
- Full Text
- View/download PDF
47. Dynamic fibroblast contractions attract remote macrophages in fibrillar collagen matrix.
- Author
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Pakshir P, Alizadehgiashi M, Wong B, Coelho NM, Chen X, Gong Z, Shenoy VB, McCulloch CA, and Hinz B
- Subjects
- Animals, Cell Adhesion immunology, Cells, Cultured, Fibroblasts metabolism, Intravital Microscopy, Macrophages metabolism, Mice, Mice, Inbred C57BL, Microscopy, Video, Primary Cell Culture, Cell Movement immunology, Extracellular Matrix metabolism, Fibrillar Collagens metabolism, Fibroblasts immunology, Macrophages immunology
- Abstract
Macrophage (Mϕ)-fibroblast interactions coordinate tissue repair after injury whereas miscommunications can result in pathological healing and fibrosis. We show that contracting fibroblasts generate deformation fields in fibrillar collagen matrix that provide far-reaching physical cues for Mϕ. Within collagen deformation fields created by fibroblasts or actuated microneedles, Mϕ migrate towards the force source from several hundreds of micrometers away. The presence of a dynamic force source in the matrix is critical to initiate and direct Mϕ migration. In contrast, collagen condensation and fiber alignment resulting from fibroblast remodelling activities or chemotactic signals are neither required nor sufficient to guide Mϕ migration. Binding of α2β1 integrin and stretch-activated channels mediate Mϕ migration and mechanosensing in fibrillar collagen ECM. We propose that Mϕ mechanosense the velocity of local displacements of their substrate, allowing contractile fibroblasts to attract Mϕ over distances that exceed the range of chemotactic gradients.
- Published
- 2019
- Full Text
- View/download PDF
48. PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease.
- Author
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Patel VB, Zhabyeyev P, Chen X, Wang F, Paul M, Fan D, McLean BA, Basu R, Zhang P, Shah S, Dawson JF, Pyle WG, Hazra M, Kassiri Z, Hazra S, Vanhaesebroeck B, McCulloch CA, and Oudit GY
- Subjects
- Animals, Dogs, Female, Gelsolin genetics, Humans, Male, Mice, Knockout, Middle Aged, Models, Cardiovascular, Phosphatidylinositol 3-Kinases metabolism, Phosphatidylinositol Phosphates metabolism, Ventricular Remodeling, Actin Cytoskeleton metabolism, Gelsolin metabolism, Heart Failure etiology, Mechanotransduction, Cellular, Myocardium metabolism
- Abstract
Biomechanical stress and cytoskeletal remodeling are key determinants of cellular homeostasis and tissue responses to mechanical stimuli and injury. Here we document the increased activity of gelsolin, an actin filament severing and capping protein, in failing human hearts. Deletion of gelsolin prevents biomechanical stress-induced adverse cytoskeletal remodeling and heart failure in mice. We show that phosphatidylinositol (3,4,5)-triphosphate (PIP3) lipid suppresses gelsolin actin-severing and capping activities. Accordingly, loss of PI3Kα, the key PIP3-producing enzyme in the heart, increases gelsolin-mediated actin-severing activities in the myocardium in vivo, resulting in dilated cardiomyopathy in response to pressure-overload. Mechanical stretching of adult PI3Kα-deficient cardiomyocytes disrupts the actin cytoskeleton, which is prevented by reconstituting cells with PIP3. The actin severing and capping activities of recombinant gelsolin are effectively suppressed by PIP3. Our data identify the role of gelsolin-driven cytoskeletal remodeling in heart failure in which PI3Kα/PIP3 act as negative regulators of gelsolin activity.
- Published
- 2018
- Full Text
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49. A novel, cell-permeable, collagen-based membrane promotes fibroblast migration.
- Author
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Sadeghi R, Mahdavi P, Lee WS, Quan B, Sone E, Ganss B, and McCulloch CA
- Subjects
- Adult, Animals, Biocompatible Materials pharmacology, Cell Proliferation drug effects, Cells, Cultured, Chemotaxis physiology, Fibronectins pharmacology, Humans, Insulin-Like Growth Factor I pharmacology, Male, Microscopy, Confocal, Permeability, Polyglactin 910 pharmacology, Rats, Rats, Sprague-Dawley, Cell Movement drug effects, Collagen pharmacology, Fibroblasts drug effects, Membranes, Artificial
- Abstract
Background and Objective: Growth factors are frequently incorporated into scaffolds to promote periodontal regeneration but many currently used scaffolds do not encourage cell migration towards the dentogingival junction. We examined the proliferation and migration of human gingival fibroblasts in a novel, physically robust, collagen-Vicryl™ membrane loaded with fibronectin (FN) and/or insulin-like growth factor (IGF-I). Biocompatibility of the membranes was evaluated in rat dorsal skin., Material and Methods: Chemotaxis was examined in Boyden chambers and cell migration by confocal imaging of membranes, which were fabricated from rat tail type I collagen with embedded Vicryl knitted mesh, IGF-I (50, 100 ng/mL) and FN (10 μg/mL). Membranes (Vicryl alone, collagen+Vicryl, collagen+Vicryl+IGF-I, collagen+Vicryl+FN') were implanted subcutaneously in 8 rats and were evaluated by histomorphometry after 7 and 14 days., Results: IGF-I (50 or 100 ng/mL) promoted chemotaxis compared with vehicle controls (P = .02, P = .001, respectively). IGF-I did not affect cell proliferation. Incorporation of FN retarded time-dependent release of IGF-I from collagen gels. Three dimensional confocal microscopy imaging of cell migration through collagen+Vicryl membranes showed enhanced migration in the IGF+FN group compared to all other groups at 8, 10 and 14 days (P < .05). In a rat skin model, implanted membranes were surrounded by thin collagen capsules and mild inflammatory infiltrates., Conclusion: Incorporation of FN into IGF-I-loaded collagen+Vicryl membranes reduced IGF release from collagen and increased the migration of human gingival fibroblasts. The new membrane may promote healing and reformation of the dentogingival junction., (© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2018
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50. The leucine-rich region of Flightless I interacts with R-ras to regulate cell extension formation.
- Author
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Arora PD, He T, Ng K, and McCulloch CA
- Subjects
- Animals, Carrier Proteins chemistry, Cattle, Collagen metabolism, DNA Helicases chemistry, Mice, Microfilament Proteins, Models, Biological, Poly-ADP-Ribose Binding Proteins chemistry, Protein Binding, RNA Helicases chemistry, RNA Recognition Motif Proteins chemistry, Signal Transduction, Trans-Activators, ras Proteins chemistry, src Homology Domains, Carrier Proteins metabolism, Cell Surface Extensions metabolism, Leucine metabolism, ras Proteins metabolism
- Abstract
Flightless I (FliI) is a calcium-dependent, actin severing and capping protein that localizes to cell matrix adhesions, contributes to the generation of cell extensions, and colocalizes with Ras. Currently, the mechanism by which FliI interacts with Ras to enable assembly of actin-based cell protrusions is not defined. R-Ras, but not K-ras, H-ras, or N-ras, associated with the leucine-rich region (LRR) of FliI. Mutations of the proline-rich region of R-ras (P202A, P203A) prevented this association. Knockdown of Ras GTPase-activating SH3 domain-binding protein (G3BP1) or Rasgap
120 by small interfering RNA inhibited the formation of cell extensions and prevented interaction of R-ras and G3BP1 in FliI wild-type (WT) cells. Pull-down assays using G3BP1 fusion proteins showed a strong association of R-ras with the C-terminus of G3BP1 (amino acids 236-466), which also required the LRR of FliI. In cells that expressed the truncated N-terminus or C-terminus of G3BP1, the formation of cell extensions was blocked. Endogenous Rasgap120 interacted with the N-terminus of G3BP1 (amino acids 1-230). We conclude that in cells plated on collagen FliI-LRR interacts with R-ras to promote cell extension formation and that FliI is required for the interaction of Rasgap120 with G3BP1 to regulate R-ras activity and growth of cell extensions.- Published
- 2018
- Full Text
- View/download PDF
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