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10. Spin trapping studies of alcohol-initiated radicals in rat liver: influence of dietary fat

Author

Reinke, Lester A. and McCay, Paul B.

Subjects
Rats -- Food and nutrition, Dietary fat -- Research, Liver diseases -- Research, Food/cooking/nutrition
Abstract

We conducted spin trapping experiments to test the effects of acute and chronic alcohol consumption in livers from rats that had been fed either high fat (35% of energy) or low fat (12% of energy) liquid diets. Rats were anesthetized with isoflurane, the spin trapping agent POBN [[Alpha]-(4-pyridyl-1-oxide)-N-t-butylnitrone] was administered by intravenous injection, and bile samples were collected for electron paramagnetic resonance (EPR) analyses. Two different types of EPR spectra were observed in bile from the animals in these studies. One set of spectral lines was from the 1-hydroxyethyl radical adduct of POBN, which was conclusively identified by injecting the rats with [1-13C]ethanol. The EPR signals of a second type of radical adduct in bile could be observed both before and after acute administration of ethanol. Although the radical(s) responsible for this second series of signals could not be conclusively identified, it is likely that lipid radicals were formed under these conditions and trapped by POBN. For both types of radical adducts, the most intense EPR signals were observed in rats that had been fed alcohol in combination with a high fat diet for 2 wk before the experiments. These results confirm and extend previous data indicating that high levels of dietary fat enhance alcohol-associated free radical formation in the liver. KEY WORDS: alcohol free radicals 1-hydroxyethyl radicals lipid radicals spin trapping rats

Published
1997

20. Glycosyltransferases (Transglycosylases.)

Author

Caputto, Ranwel, Trucco, Raul E., McCay, Paul B., Hoppe-Seyler, Thierfelder, Lang, Konrad, editor, Lehnartz, Emil, editor, Bruns, F. H., editor, Caputto, R., editor, Diekmann, H., editor, Ehrenreich, R., editor, Faillard, H., editor, Gibian, H., editor, Hardegg, W., editor, Heinz, F., editor, Hoffmann-Ostenhof, O., editor, Huennekens, F. M., editor, Kesselring, K., editor, Lamprecht, W., editor, Lang, K., editor, McCay, P. B., editor, Pfannemüller, B., editor, Ratner, S., editor, Reinauer, H., editor, Rohdewald, M., editor, Róka, L., editor, Schievelbein, H., editor, Scrimgeour, K. G., editor, Siebert, G., editor, Trautschold, I., editor, Trucco, R. E., editor, Turvey, J. R., editor, Wallenfels, K., editor, Weber, F., editor, Werle, E., editor, Wieland, O., editor, Williams, J. N., Jr., editor, and Wiss, O., editor

Published
1966
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21. Glycosyltransferases

Author

Caputto, Ranwel, Trucco, Raul E., McCay, Paul B., Lang, Konrad, editor, Lehnartz, Emil, editor, Hoffmann-Ostenhof, Otto, editor, Siebert, Günther, editor, Bruns, F. H., editor, Caputto, R., editor, Diekmann, H., editor, Ehrenreich, R., editor, Faillard, H., editor, Gibian, H., editor, Hardegg, W., editor, Heinz, F., editor, Hoffmann-Ostenhof, O., editor, Huennekens, F. M., editor, Kesselring, K., editor, Lamprecht, W., editor, Lang, K., editor, McCay, P. B., editor, Pfannemüller, B., editor, Ratner, S., editor, Reinauer, H., editor, Rohdewald, M., editor, Róka, L., editor, Schievelbein, H., editor, Scrimgeour, K. G., editor, Siebert, G., editor, Trautschold, I., editor, Trucco, R. E., editor, Turvey, J. R., editor, Wallenfels, K., editor, Weber, F., editor, Werle, E., editor, Wieland, O., editor, Williams, J. N., Jr., editor, and Wiss, O., editor

Published
1966
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47. Spin Trapping and Electron Paramagnetic Resonance Spectroscopy.

Author

Walker, John M., Armstrong, Donald, Brackett, Daniel J., Wallis, Gemma, Wilson, Michael F., and McCay, Paul B.

Abstract

Electron paramagnetic resonance (EPR) spectroscopy methodology is a highly selective and sensitive assay for detecting paramagnetic species. Owing to the unpaired electron in the outer orbit, free radicals are paramagnetic species and, when in sufficient quantity, are directly detectable and measurable using EPR spectroscopy. However, many free-radicals species are highly reactive, with relatively short half-lives, and the concentrations found in biochemical systems are usually inadequate for direct detection by EPR spectroscopy. Spin-trapping is a chemical reaction that provides an approach to help overcome this problem. Spin traps are compounds that react covalently with highly transient free radicals to form relatively stable, persistent spin adducts that also possess paramagnetic resonance spectra detectable by EPR spectroscopy. When a spin trap is added to a free radical-generating biochemical reaction, a growing pool of relatively long-lived spin adducts is created as the free radicals react with the spin trap. Detectable EPR spectra are generated by the reaction when the signal strength of the accumulation of adducts reaches the lower limit of sensitivity of the particular spectrometer being utilized. [ABSTRACT FROM AUTHOR]

Published
1998
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