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34 results on '"McCaustland, Karen"'

1. Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome

Author

Rota, Paul A., Oberste, M. Steven, Monroe, Stephan S., Nix, W. Allan, Campagnoli, Ray, Icenogle, Joseph P., Peñaranda, Silvia, Bankamp, Bettina, Maher, Kaija, Chen, Min-hsin, Tong, Suxiong, Tamin, Azaibi, Lowe, Luis, Frace, Michael, DeRisi, Joseph L., Chen, Qi, Wang, David, Erdman, Dean D., Burns, Cara, Ksiazek, Thomas G., Rollin, Pierre E., Sanchez, Anthony, Liffick, Stephanie, Holloway, Brian, Limor, Josef, McCaustland, Karen, Olsen-Rasmussen, Melissa, Fouchier, Ron, Günther, Stephan, Drosten, Christian, Pallansch, Mark A., Anderson, Larry J., and Bellini, William J.

Published
2003

4. Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus

Author

Emery, Shannon L., Erdman, Dean D., Bowen, Michael D., Newton, Bruce R., Winchell, Jonas M., Meyer, Richard F., Tong, Suxiang, Cook, Byron T., Holloway, Brian P., McCaustland, Karen A., Rota, Paul A., Bankamp, Bettina, Lowe, Luis E., Ksiazek, Tom G., Bellini, William J., and Anderson, Larry J.

Abstract

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located [...]

Published
2004

8. Real-Time PCR: an Essential Guide

Author

McCaustland, Karen

Subjects
Real-Time Polymerase Chain Reaction (Book) -- Book reviews, Books -- Book reviews
Abstract

Kirsten Edwards, Julie Logan, and Nick Saunders Horizon Bioscience, Norfolk, United Kingdom ISBN: 0-954232-7-X Pages: 346, Price: 95 [pounds sterling]; U.S. $180 Real-time polymerase chain reaction (PCR) technique has advanced [...]

Published
2005

10. Evaluation and Improvement of Real-Time PCR Assays Targeting lytA , ply , and psaA Genes for Detection of Pneumococcal DNA

Author

Carvalho, Maria da Gloria S., primary, Tondella, Maria Lucia, additional, McCaustland, Karen, additional, Weidlich, Luciana, additional, McGee, Lesley, additional, Mayer, Leonard W., additional, Steigerwalt, Arnold, additional, Whaley, Melissa, additional, Facklam, Richard R., additional, Fields, Barry, additional, Carlone, George, additional, Ades, Edwin W., additional, Dagan, Ron, additional, and Sampson, Jacquelyn S., additional

Published
2007
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29. Evaluation and Improvement of Real-Time PCR Assays Targeting lytA, ply, and psaAGenes for Detection of Pneumococcal DNA

Author

Carvalho, Maria da Gloria S., Tondella, Maria Lucia, McCaustland, Karen, Weidlich, Luciana, McGee, Lesley, Mayer, Leonard W., Steigerwalt, Arnold, Whaley, Melissa, Facklam, Richard R., Fields, Barry, Carlone, George, Ades, Edwin W., Dagan, Ron, and Sampson, Jacquelyn S.

Abstract

ABSTRACTThe accurate diagnosis of pneumococcal disease has frequently been hampered not only by the difficulties in obtaining isolates of the organism from patient specimens but also by the misidentification of pneumococcus-like viridans group streptococci (P-LVS) as Streptococcus pneumoniae. This is especially critical when the specimen comes from the respiratory tract. In this study, three novel real-time PCR assays designed for the detection of specific sequence regions of the lytA, ply, and psaAgenes were developed (lytA-CDC, ply-CDC, and psaA, respectively). These assays showed high sensitivity (<10 copies for lytA-CDC and ply-CDC and an approximately twofold less sensitivity for psaA). Two additional real-time PCR assays for lytAand plydescribed previously for pneumococcal DNA detection were also evaluated. A panel of isolates consisting of 67 S. pneumoniaeisolates (44 different serotypes and 3 nonencapsulated S. pneumoniaeisolates from conjunctivitis outbreaks) and 104 nonpneumococcal isolates was used. The 67 S. pneumoniaeisolates were reactive in all five assays. The new real-time detection assays targeting the lytAand psaAgenes were the most specific for the detection of isolates confirmed to be S. pneumoniae, with lytA-CDC showing the greatest specificity. Both plyPCRs were positive for all isolates of S. pseudopneumoniae, along with 13 other isolates of other P-LVS isolates confirmed to be non-S. pneumoniaeby DNA-DNA reassociation. Thus, the use of the plygene for the detection of pneumococci can lead to false-positive reactions in the presence of P-LVS. The five assays were applied to 15 culture-positive cerebrospinal fluid specimens with 100% sensitivity; and serum and ear fluid specimens were also evaluated. Both the lytA-CDC and psaAassays, particularly the lytA-CDC assay, have improved specificities compared with those of currently available assays and should therefore be considered the assays of choice for the detection of pneumococcal DNA, particularly when upper respiratory P-LVS might be present in the clinical specimen.

Published
2007
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30. Posttransfusion non-A, non-B hepatitis in chimpanzees

Author

Bradley, Daniel W., McCaustland, Karen A., Cook, E.H., Schable, Charles A., Ebert, James W., and Maynard, James E.

Abstract

Posttransfusion non-A, non-B hepatitis associated with the formation of hepatocyte cytoplasmic tubules was experimentally transmitted to chimpanzees by intravenous inoculation of a proven-infectious plasma that had been pelleted and microfiltrated, or purified by a combination of pelleting and rate-zonal banding. The results of these studies indicate that a factor VIII-derived non-A, non-B tubule-forming agent will pass through an 80-nm membrane filter and that it can be recovered from infected plasma by use of a purification procedure that assumes the non-A, non-B tubule-forming agent is a small, enveloped virus. Our findings, in combination with the known sensitivity of the non-A, non-B tubule-forming agent to chloroform and its apparent lack of nucleic acid homology with hepatitis B virus, further suggest that at least one etiologic agent of human posttransfusion non-A, non-B hepatitis may be a small, enveloped RNA virus.

Published
1985
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34. Real-Time PCR: An Essential Guide.

Author

Edwards, Kirsten, Logan, Julie, Saunders, Nick, and McCaustland, Karen

Subjects
POLYMERASE chain reaction, NONFICTION
Abstract

Reviews the book "Real-Time PCR: An Essential Guide," by Kirsten Edwards, Julie Logan and Nick Saunders.

Published
2005

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