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Your search keyword '"Mazurkova, Natalia A."' showing total 24 results
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24 results on '"Mazurkova, Natalia A."'

7. Cacalia hastata L.: anti-influenza activity and phytochemical characteristics of extracts

Author

Lobanova Irina, Filippova Ekaterina, Kotsupiy Olga, Protsenko Maria, Kharina Tatiana, and Mazurkova Natalia

Subjects
Microbiology, QR1-502, Physiology, QP1-981, Zoology, QL1-991
Abstract

This is the first study to assess antiviral activity against human influenza virus A/Aichi/2/68 and bird influenza virus A/chicken/Kurgan/05/2005 and phytochemical characteristics of an ethanol extract of Cacalia hastata L. from wild populations growing in the vicinity of Tomsk, Russia (mixed forest). A log10 neutralization index—an indicator of inhibition of virus replication by the extract from C. hastata leaves—was ≤2 lg for each influenza virus. The extract reduced the infectivity of the human influenza virus by 1.5-fold and that of the avian influenza virus by 1.7-fold. The phytochemical characterization of C. hastata (its various organs) showed that the largest amount of the tested biologically active substances is present in leaves and rhizomes.

Published
2021
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8. Roots of Rosa majalisHerrm. as a source of antioxidants and anti-influenza agents

Author

Karpova, Evgeniya A., Kostikova, Vera A., Khramova, Elena P., Shaldaeva, Tatiana M., Vasil’eva, Olga Yu., Mazurkova, Natalia A., Filippova, Ekaterina I., Mazurkov, Oleg Yu., and Makarevich, Elena V.

Abstract

Rosa majalisHerrm. (Rosaceae) is a shrub widespread throughout Eurasia. In West Siberia, it is cultivated as an ornamental plant and raw material for the manufacture of fruit multivitamin concentrates. Meanwhile, metabolites and biological activities of its roots are poorly studied. The aim of this investigation was to evaluate the potential of ethyl acetate (EtOAc), butanol (ButOH), aqueous ethanol (EtOH), and aqueous (H2O) extracts of R. majalisroots as sources of antioxidant and anti-influenza compounds. Spectrophotometry and HPLC were used for metabolite quantification. Antioxidant activities were determined via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The antiviral activities were assessed as half-maximal inhibitory concentrations (IC50). The EtOAc extract manifested the highest concentrations of phenolic compounds (53.79 mg g−1of dry extract) and saponins (48.92 mg g−1). Total gallic acid was the main phenolic compound in all extracts (24.98–38.68 mg g−1). Catechin, free ellagic acid, and total ellagic acid had the highest levels in the EtOAc, H2O, and EtOH extracts, respectively (30.83, 22.38, and 34.95 mg g−1). The EtOAc extract showed the highest free-radical scavenging activity (2.31 trolox equivalents) and anti-influenza effects (IC5061.8 and 50.3 µg mL−1) against A/Aichi/2/68 (H3N2) and A/Chicken/05/2005 (H5N1), respectively. Our findings indicate good potential of these extracts for drug design against free-radical damage and influenza virus.

Published
2024
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9. Safety and Pharmacokinetics of the Substance of the Anti-Smallpox Drug NIOCH-14 after Oral Administration to Laboratory Animals

Author

Shishkina, Larisa N., primary, Mazurkov, Oleg Yu., additional, Bormotov, Nikolai I., additional, Skarnovich, Maksim O., additional, Serova, Olga A., additional, Mazurkova, Natalia A., additional, Skarnovich, Maria A., additional, Chernonosov, Alexander A., additional, Selivanov, Boris A., additional, Tikhonov, Alexey Ya., additional, Gamaley, Svetlana G., additional, Shimina, Galina G., additional, Sysoyeva, Galina M., additional, Taranov, Oleg S., additional, Danilenko, Elena D., additional, Agafonov, Alexander P., additional, and Maksyutov, Rinat A., additional

Published
2023
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23. Nitraria schoberiL. hairy root culture as a source of compounds with antiviral activity against influenza virus subtypes А(H5N1) and А(H3N2)

Author

Zheleznichenko, Tatiana, Banaev, Evgenii, Asbaganov, Sergey, Voronkova, Maria, Kukushkina, Tatiana, Filippova, Ekaterina, Mazurkova, Natalia, Shishkina, Larisa, and Novikova, Tatyana

Abstract

The hairy root culture of halophyte Nitraria schoberiwas obtained by transforming seedlings’ primary leaves with a wild strain of Agrobacterium rhizogenes15834 SWISS (transformation frequency was 78.7%). The roots’ transgenic status was confirmed by PCR analysis using rolB-, rolC-, virC-, virD1-specific primers. There was a significant increase in the biosynthesis of flavonoids, hydroxycinnamic acids, pectins, protopectins and saponins in hairy roots as compared to intact plant roots. The сatechin content in ethanol extracts of transformed roots’ exceeded this factor 3.8 times in plant roots, but the compound composition of catechin nature in the latter was more diverse. The high antiviral activity of N. schoberihairy root ethanol extracts against influenza virus subtypes A(H5N1), A(H3N2) was revealed, which manifested itself in the effective inhibition of infectivity both in vitro—in MDCK cell culture—and in vivo increasing survival of mice infected with them.

Published
2018
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24. High antiviral effect of TiO 2 ·PL-DNA nanocomposites targeted to conservative regions of (-)RNA and (+)RNA of influenza A virus in cell culture.

Author

Levina AS, Repkova MN, Bessudnova EV, Filippova EI, Mazurkova NA, and Zarytova VF

Abstract

Background: The development of new antiviral drugs based on nucleic acids is under scrutiny. An important problem in this aspect is to find the most vulnerable conservative regions in the viral genome as targets for the action of these agents. Another challenge is the development of an efficient system for their delivery into cells. To solve this problem, we proposed a TiO 2 ·PL-DNA nanocomposite consisting of titanium dioxide nanoparticles and polylysine (PL)-containing oligonucleotides. Results: The TiO 2 ·PL-DNA nanocomposites bearing the DNA fragments targeted to different conservative regions of (-)RNA and (+)RNA of segment 5 of influenza A virus (IAV) were studied for their antiviral activity in MDCK cells infected with the H1N1, H5N1, and H3N2 virus subtypes. Within the negative strand of each of the studied strains, the efficiency of DNA fragments increased in the direction of its 3'-end. Thus, the DNA fragment aimed at the 3'-noncoding region of (-)RNA was the most efficient and inhibited the reproduction of different IAV subtypes by 3-4 orders of magnitude. Although to a lesser extent, the DNA fragments targeted at the AUG region of (+)RNA and the corresponding region of (-)RNA were also active. For all studied viral subtypes, the nanocomposites bearing the DNA fragments targeted to (-)RNA appeared to be more efficient than those containing fragments aimed at the corresponding (+)RNA regions. Conclusion: The proposed TiO 2 ·PL-DNA nanocomposites can be successfully used for highly efficient and site-specific inhibition of influenza A virus of different subtypes. Some patterns of localization of the most vulnerable regions in IAV segment 5 for the action of DNA-based drugs were found. The (-)RNA strand of IAV segment 5 appeared to be more sensitive as compared to (+)RNA.

Published
2016
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