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Gray mold, caused by Botrytis cinerea Pers. Fr., is one of the most vital plant diseases, causing extensive pre- and post-harvest losses in apple fruits. In the current study, we isolated and identified two potential endophytic bioagents, Bacillus subtilis and Streptomyces endus. Both bioagents exhibited a potent fungistatic effect against B. cinerea under both in vitro and in planta conditions. Moreover, two experiments were carried out; (i) the first experiment was conducted at room temperature after artificial inoculation with B. cinerea to monitor the progression of the infection and the corresponding biochemical responses of the apples. Our in vivo findings showed that the treated B. cinerea-infected apple fruits with the cell-free bacterial filtrate of B. subtilis and S. endus (dipping or wrapping) significantly reduced the rotten area of the treated apple at room temperature. Additionally, B. subtilis and S. endus enhanced the enzymatic (POX and PPO) and non-enzymatic (phenolics and flavonoids) antioxidant defense machinery in treated apples. (ii) The second experiment focused on the preventive effects of both bioagents over a 90-day storage period at 1 °C of healthy apples (no artificial inoculation). The application of both bacterial filtrates prolonged the storage period, reduced the relative weight loss, and maintained high-quality parameters including titratable acidity, firmness, and total soluble solids of apple fruits under cold storage at 1 °C. The Kaplan–Meier analysis of rotten apples over 90 days during cold storage showed that the treated apples lasted longer than the non-treated apples. Moreover, the lifespan of apple fruits dipped in the culture filtrate of B. subtilis, or a fungicide, was increased, with no significant differences, compared with the non-treated apples. The current results showed the possibility of using both bioagents as a safe and eco-friendly alternative to chemical fungicides to control gray mold disease in apples. [ABSTRACT FROM AUTHOR]

White mold, caused by the necrotrophic fungus Sclerotinia sclerotiorum, is a challenging disease to common bean cultivation worldwide. In the current study, two non-proteinogenic amino acids (NPAAs), γ-aminobutyric acid (GABA) and ß-alanine, were suggested as innovative environmentally acceptable alternatives for more sustainable management of white mold disease. In vitro, GABA and ß-alanine individually demonstrated potent dose-dependent fungistatic activity and effectively impeded the radial growth and development of S. sclerotiorum mycelium. Moreover, the application of GABA or ß-alanine as a seed treatment followed by three root drench applications efficiently decreased the disease severity, stimulated plant growth, and boosted the content of photosynthetic pigments of treated S. sclerotiorum-infected plants. Furthermore, although higher levels of hydrogen peroxide (H2O2), superoxide anion (O2•-), and malondialdehyde (MDA) indicated that S. sclerotiorum infection had markedly triggered oxidative stress in infected bean plants, the exogenous application of both NPAAs significantly reduced the levels of the three studied oxidative stress indicators. Additionally, the application of GABA and ß-alanine increased the levels of both non-enzymatic (total soluble phenolics and flavonoids), as well as enzymatic (catalase [CAT], peroxidases [POX], and polyphenol oxidase [PPO]) antioxidants in the leaves of S. sclerotioruminfected plants and improved their scavenging activity and antioxidant efficiency. Applications of GABA and ß-alanine also raised the proline and total amino acid content of infected bean plants. Lastly, the application of both NPAAs upregulated the three antioxidant-related genes PvCAT1, PvCuZnSOD1, and PvGR. Collectively, the fungistatic activity of NPAAs, coupled with their ability to alleviate oxidative stress, enhance antioxidant defenses, and stimulate plant growth, establishes them as promising ecofriendly alternatives for white mold disease management for sustainable bean production. [ABSTRACT FROM AUTHOR]

The current study aimed to design and test the accuracy of a variable-rate control metering mechanism (VRCMM) and a remote smart monitoring system (RSMS) for a precision sugarcane transplanter based on IoT technology. The VRCMM is used to operate the seedling metering device at different speeds using a stepper motor based on the travel speed, and the RSMS was employed to evaluate of the three basic parameters of seedling amount, optimum rate, and missed rate. Two types of sensors were used for detecting the sugarcane seedling (SS) and travel speed, including one ultrasonic sensor and one infrared RPM sensor. The study was performed at five travel speeds and four transplant spacings. The findings of laboratory tests showed that the mean record of the relative error between the desired stepper motor speed of the VRCMM and the real value was 3.39%, and it increased with increasing the travel speed. as Additionally, the speed regulation performance was in agreement with the transplanting index. The change in RSMS accuracy is obvious when the travel speed is high and the transplant spacing is small. The RSMS accuracy drops sharply, revealing a leaping change. In conclusion, the smart and intelligent designed sugarcane transplanter would be very useful in sugarcane production. [ABSTRACT FROM AUTHOR]

The Halayeb area is one of the most important Egyptian places, occupying a portion of the country's southeastern desert zone and serving as a strategic border with Sudan. The major goal of this study is to combine existing data on landforms, soil qualities, and climate data to define agro-ecological zones (AEZ) that are suitable for agricultural development. Thirty-two soil profiles were dug throughout the study region to represent physiographic units, and a digital soil map was generated based on an analysis of the digital elevation model and Landsat 8 satellite data, as well as climatic data from nine sites within the study area. Using the Model Builder in ArcGIS software, AEZ were created based on overlay maps of topography, soil chemical and physical properties, temperature, and precipitation maps. Zone (I) represents highly suitable areas (2.6 percent), zone (II) represents suitable areas (12.4 percent), zone (III) represents moderately suitable areas (26.5 percent), and zone (IV) represents marginally suitable areas (37.3 percent). highly suitable(S1), suitable (S2), and moderately suitable(S3) were the most appropriate classes for all of the crops studied. The findings will aid decision-makers in developing various development plans based on the research area's conditions. The study's methodology and findings can be used to evaluate which land is best suited for agricultural productivity growth. [ABSTRACT FROM AUTHOR]

Pot experiments were conducted during 2017 and 2018 seasons to study the effect of Actosol, Actosol+Zinc and Actosol+Silica nanoparticles on Morpho-physiological and yield characteristics of two rice varieties (Giza 177 salt-susceptible and Giza179 salt-tolerant) under salinity conditions. Application of Actosol, Actosol+Nano Zn and Actosol+Nano Si minimized the bad effect of salinity in salt stressed treated plants compared with control of two rice varieties in both seasons. Giza 179 gave the best results of root fresh weight, root length, plant height, number of tillers, panicle number, 1000-grain weight, number of filled grains and grain yield plant-1 of the treated plants with Actosol under salinity level at 4000 ppm, while salinity (6000 ppm) recorded the lowest values of the most studied characters compared with Actosol+Nano Zn and Actosol+Nano Si treatments. The highest levels of CAT and POX were obtained with Actosol+Nano Si compared with other treatments in Giza 179. [ABSTRACT FROM AUTHOR]

The current experiments were conducted during two successive seasons, (2017/2018 and 2018/2019) to study the susceptibility of some faba bean varieties to the main insect pests attacking faba bean. Resistant varieties are effective and possible alternative technique to control insect pests according to their safeness and eco-friendly characters, which is considered a protection method to reduce the use of harmful synthetic insecticides. Our results indicated that based on susceptibility degree, faba bean varieties, were categorized into: the susceptible group, which contains Sakha3. The low resistant one contains Sakha4 and Giza843. The moderate resistant group observed in Noubaria1. The susceptible one with the highest average number of Aphis craccivora (Koch.), Bemisia tahaci (Genn.), Nezara viridula (L.), Emposaca spp. and Liriomyza congesta (Becker) recorded the highest of total nitrogen, phosphorus, potassium, zinc, iron, total carbohydrates, crude protein, and total chlorophyll while received the lowest of manganese, total lipids, acidity, total phenol, silica, catalase and peroxidase en- zymes, as compared with the moderately resistance one which was almost the least infested with the aforementioned insects, as it had the least values of nitrogen, phosphorus, potassium, zinc, iron, carbohydrates, crude protein, and chlorophyll and the highest values of manganese, lipids, acidity, phenol, silica, catalase and peroxidase enzymes. Statistical analysis showed significant or highly significant positive relationship between varieties contents (total nitrogen, phosphorus, potassium, zinc, iron, total carbohydrates, crude protein and totalchlorophyll) with all tested insects, while was highly significant negative relationship with of manganese, lipids, acidity, phenol, silica, catalase and peroxidase en zymes. The yield and yield characters were negatively affected in susceptible varieties more than the low resistant and moderate resistant groups. [ABSTRACT FROM AUTHOR]

Phenotypic plasticity is frequently highlighted as a key factor in plant invasiveness, as it enables invasive species to adapt to diverse, complicated habitats. Trianthema portulacastrum is one of the most common aggressive species that threaten different crops around the world. Phenotypic plasticity in T. portulacastrum was investigated by comparing variation in germination, vegetative macromorphology, photosynthetic pigments, stomatal complexes, and seed micromorphological traits of 35 samples collected from 35 different localities. One-way cluster analysis and principal component analysis (PCA) were used to classify samples into homogeneous groups based on the measured traits. Pairwise statistical comparisons were conducted between the three resulting groups. The phenotypic plasticity index (PI) was calculated and compared among different groups of characters. Results showed that photosynthetic pigments and macromorphological characteristics had the highest PI, followed by seed micromorphology, and then stomatal complex traits, while germination parameters showed the lowest PI. We propose that soil moisture, salinity, and temperature are the most determinative and explanative variables of the variation between the three classified groups. We strongly believe that the phenotypic plasticity of T. portulacastrum will support species abundance and spread even under expected changes in climatic conditions, in contrast to the vulnerable traditional crops. [ABSTRACT FROM AUTHOR]

The phytopathogenic basidiomycetous fungus, Rhizoctonia solani, has a wide range of host plants including members of the family Poaceae, causing damping-off and root rot diseases. In this study, we biosynthesized spherical-shaped silicon dioxide nanoparticles (SiO2 NPs; sized between 9.92 and 19.8 nm) using saffron extract and introduced them as a potential alternative therapeutic solution to protect wheat seedlings against R. solani. SiO2 NPs showed strong dose-dependent fungistatic activity on R. solani, and significantly reduced mycelial radial growth (up to 100% growth reduction), mycelium fresh and dry weight, and pre-, post-emergence damping-off, and root rot severities. Moreover, the impact of SiO2 NPs on the growth of wheat seedlings and their potential mechanism (s) for disease suppression was deciphered. SiO2 NPs application also improved the germination, vegetative growth, and vigor indexes of infected wheat seedlings which indicates no phytotoxicity on treated wheat seedlings. Moreover, SiO2 NPs enhanced the content of the photosynthetic pigments (chlorophylls and carotenoids), induced the accumulation of defense-related compounds (particularly salicylic acid), and alleviated the oxidative stress via stimulation of both enzymatic (POD, SOD, APX, CAT, and PPO) and non-enzymatic (phenolics and flavonoids) antioxidant defense machinery. Collectively, our findings demonstrated the potential therapeutic role of SiO2 NPs against R. solani infection via the simultaneous activation of a multilayered defense system to suppress the pathogen, neutralize the destructive effect of ROS, lipid peroxidation, and methylglyoxal, and maintain their homeostasis within R. solani-infected plants. [ABSTRACT FROM AUTHOR]

Citrus is grown globally throughout the subtropics and semi-arid to humid tropics. Abiotic stresses such as soil water deficit negatively affect plant growth, physiology, biochemistry, and anatomy. Herein, we investigated the effect(s) of three water regimes (control, moderate drought, and severe drought) on the physiological and anatomical structure of 10 different citrus rootstocks with different degrees of tolerance to drought stress. Brazilian sour orange and Gadha dahi performed well by avoiding desiccation and maintaining plant growth, plant water status, and biochemical characters, while Rangpur Poona nucellar (C. limonia) and Sunki × bentake were the most sensitive rootstocks at all stress conditions. At severe water stress, the highest root length (24.33 ± 0.58), shoot length (17.00 ± 1.00), root moisture content (57.67 ± 1.53), shoot moisture content (64.59 ± 1.71), and plant water potential (−1.57 ± 0.03) was observed in tolerant genotype, Brazilian sour orange. Likewise, chlorophyll a (2.70 ± 0.06), chlorophyll b (0.87 ± 0.06) and carotenoids (0.69 ± 0.08) were higher in the same genotype. The lowest H2O2 content (77.00 ± 1.00) and highest proline content (0.51 ± 0.06) were also recorded by Brazilian sour orange. The tolerance mechanism of tolerant genotypes was elucidated by modification in anatomical structures. Stem anatomy at severe drought, 27.5% increase in epidermal cell thickness, 25.4% in vascular bundle length, 30.5% in xylem thickness, 27.7% in the phloem cell area, 8% in the pith cell area, and 43.4% in cortical thickness were also observed in tolerant genotypes. Likewise, leaf anatomy showed an increase of 27.9% in epidermal cell thickness, 11.4% in vascular bundle length, 21% in xylem thickness, and 15% in phloem cell area in tolerant genotypes compared with sensitive ones. These modifications in tolerant genotypes enabled them to maintain steady nutrient transport while reducing the risk of embolisms, increasing water-flow resistance, and constant transport of nutrients across. [ABSTRACT FROM AUTHOR]

This study analyzes the impact of environmental disclosure, board attributes, and firms' specifics on the levels of environmental and ESG performance in Europe and Asia. The study utilizes secondary data from Refinitiv Eikon database for 8094 firms for the period between 2016 and 2021. The study employs panel data analysis using fixed effect models to estimate the results. The findings suggest that disclosure on emissions, innovations, environmental controversies, environmentally friendly products, proactive environmental investments, environmental expenses, and fines charged by authorities have a positive and significant influence on the level of firms' environmental and ESG performance. Furthermore, the study identifies board tenure, independence, size, and meetings as being associated with greater levels of environmental disclosures, reporting, and sustainability score. However, board diversity is found to have a limited contribution to environmental disclosures, especially in Asian countries. Additionally, the results reveal that companies with higher revenue growth, larger size and market capitalization, and better performance have greater and better disclosure of environmental and sustainability issues. The study provides practical implications for policymakers to establish comprehensive guidelines for environmental and sustainability reporting based on the analysis of institutional, regulatory frameworks, legislation, and sustainability score enforcement status of the country., Competing Interests: The authors of the article “The Impact of Corporate Environmental Disclosure Practices and Board Attributes on Sustainability: Empirical evidence from Asia and Europe” declare the following interests statement: “The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.", (© 2023 The Authors.)

Silicon is one of the most significant elements in plants under abiotic stress, so we investigated the role of silicon in alleviation of the detrimental effects of salinity at two concentrations (1500 and 3000 ppm sodium chloride) in sweet pepper plants in two seasons (2018 and 2019). Our results indicated that relative water content, concentrations of chlorophyll a and b, nitrogen, phosphorus and potassium contents, number of fruits plant -1 , fruit fresh weight plant -1 (g) and fruit yield (ton hectare -1 ) significantly decreased in salt-stressed sweet pepper plants as compared to control plants. In addition, electrolyte leakage, proline, lipid peroxidation, superoxide (O 2 - ) and hydrogen peroxide (H 2 ) levels, soluble sugars, sucrose, and starch content as well as sodium content significantly increased under salinity conditions. Conversely, foliar application of silicon led to improvements in concentrations of chlorophyll a and b and mineral nutrients, water status, and fruit yield of sweet pepper plants. Furthermore, lipid peroxidation, electrolyte leakage, levels of superoxide, and hydrogen peroxide were decreased with silicon treatments.2 ) levels, soluble sugars, sucrose, and starch content as well as sodium content significantly increased under salinity conditions. Conversely, foliar application of silicon led to improvements in concentrations of chlorophyll a and b and mineral nutrients, water status, and fruit yield of sweet pepper plants. Furthermore, lipid peroxidation, electrolyte leakage, levels of superoxide, and hydrogen peroxide were decreased with silicon treatments.