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3 results on '"Mayu Imamura"'

1. Crosstalk between Cancer Cells and Fibroblasts for the Production of Monocyte Chemoattractant Protein-1 in the Murine 4T1 Breast Cancer

Author

Mayu Imamura, Tiantian Li, Chunning Li, Masayoshi Fujisawa, Naofumi Mukaida, Akihiro Matsukawa, and Teizo Yoshimura

Subjects
breast cancer, chemokine, lung metastasis, fibroblasts, macrophages, tumor microenvironment, Biology (General), QH301-705.5
Abstract

The chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2) is shown to promote the progression of breast cancer. We previously identified cancer cell-derived granulocyte-macrophage colony-stimulating factor (GM-CSF) as a potential regulator of MCP-1 production in the murine 4T1 breast cancer, but it played a minimum role in overall MCP-1 production. Here, we evaluated the crosstalk between 4T1 cells and fibroblasts. When fibroblasts were co-cultured with 4T1 cells or stimulated with the culture supernatants of 4T1 cells (4T1-sup), MCP-1 production by fibroblasts markedly increased. 4T1 cells expressed mRNA for platelet-derived growth factor (PDGF)-a, b and c, and the PDGF receptor inhibitor crenolanib almost completely inhibited 4T1-sup-induced MCP-1 production by fibroblasts. However, PDGF receptor antagonists failed to reduce MCP-1 production in tumor-bearing mice. Histologically, 4T1 tumors contained a small number of αSMA-positive fibroblasts, and Mcp-1 mRNA was mainly associated with macrophages, especially those surrounding necrotic lesions on day 14, by in situ hybridization. Thus, although cancer cells have the capacity to crosstalk with fibroblasts via PDGFs, this crosstalk does not play a major role in MCP-1 production or cancer progression in this model. Unraveling complex crosstalk between cancer cells and stromal cells will help us identify new targets to help treat breast cancer patients.

Published
2021
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2. pufC Gene Targeted PCR Primers for Identification and Classification of Marine Photosynthetic Bacterium Rhodovulum sulfidophilum

Author

Nao Yamauchi, Shuhei Hayashi, Tomomi Kurayama, Mayu Imamura, Shinjiro Yamamoto, Aoi Koga, Ranko Iwai, Mina Urata, and Hitoshi Miyasaka

Subjects
Genetics, Complementary and alternative medicine, biology, Pharmaceutical Science, Pharmacology (medical), Identification (biology), biology.organism_classification, Photosynthetic bacterium, Gene, Rhodovulum sulfidophilum
Published
2021
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3. Cancer Cell-Derived Granulocyte-Macrophage Colony-Stimulating Factor Is Dispensable for the Progression of 4T1 Murine Breast Cancer

Author

Chunning Li, Masayoshi Fujisawa, Tsuyoshi Shiina, Naofumi Mukaida, Kaoru Nakamura, Akihiro Matsukawa, Mayu Imamura, Tiantian Li, and Teizo Yoshimura

Subjects
Chemokine, Mammary Neoplasms, Animal, chemokines, Biology, Article, Catalysis, Inorganic Chemistry, breast cancer, Cell Line, Tumor, medicine, Animals, Macrophage, CCL17, tumor microenvironment, RNA, Messenger, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Cell Proliferation, Mice, Inbred BALB C, Tumor microenvironment, Base Sequence, Organic Chemistry, Granulocyte-Macrophage Colony-Stimulating Factor, Cancer, General Medicine, medicine.disease, cytokines, Clone Cells, Neoplasm Proteins, Computer Science Applications, macrophages, Gene Expression Regulation, Neoplastic, Granulocyte macrophage colony-stimulating factor, RANKL, inflammation, Splenomegaly, Cancer cell, Disease Progression, Cancer research, biology.protein, Female, CRISPR-Cas Systems, Granulocytes, medicine.drug
Abstract

We previously reported that 4T1 murine breast cancer cells produce GM-CSF that up-regulates macrophage expression of several cancer promoting genes, including Mcp-1/Ccl2, Ccl17 and Rankl, suggesting a critical role of cancer cell-derived GM-CSF in cancer progression. Here, we attempted to define whether 4T1 cell-derived GM-CSF contributes to the expression of these genes by 4T1tumors, and their subsequent progression. Intraperitoneal injection of anti-GM-CSF neutralizing antibody did not decrease the expression of Mcp-1, Ccl17 or Rankl mRNA by 4T1 tumors. To further examine the role of cancer cell-derived GM-CSF, we generated GM-CSF-deficient 4T1 cells by using the Crisper-Cas9 system. As previously demonstrated, 4T1 cells are a mixture of cells and cloning of cells by itself significantly reduced tumor growth and lung metastasis. By contrast, GM-CSF-deficiency did not affect tumor growth, lung metastasis or the expression of these chemokine and cytokine genes in tumor tissues. By in-situ hybridization, the expression of Mcp-1 mRNA was detected in both F4/80-expressing and non-expressing cells in tumors of GM-CSF-deficient cells. These results indicate that cancer cell-derived GM-CSF is dispensable for the tuning of the 4T1 tumor microenvironment and the production of MCP-1, CCL17 or RANKL in the 4T1 tumor microenvironment is likely regulated by redundant mechanisms.

Published
2019

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