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Innovative methods for effectively sequestering significant amounts of carbon dioxide (CO2) are required to address the ongoing problem of greenhouse gas emissions, which have increased in tandem with industrial development. Optimisation of the performance of the photobioreactor remains a challenge, despite the potential of using photobioreactors and cyanobacteria for bio sequestration. A potential solution lies in the development of a hybrid photobioreactor design. Thus, this study aims to develop a hybrid design of the photobioreactor equipped with tailor-made real-time monitoring system known as FCB2022 for cultivating Spirulina platensis. The performance is evaluated by monitoring real-time data such as pH, dissolved oxygen (DO), and temperature, alongside assessing the growth kinetic of cyanobacterium, CO2sequestration, oxygen release rate and mass carbon balance. These assessments are conducted under varying photon flux densities (PFD) of 100µmol/ (m2.s), 200µmol/ (m2.s), and 300µmol/ (m2.s). The result demonstrates that FCB2022 exhibited high hydrodynamic performance with parameters like mixing time, circulation time, Reynold number, and empty bed residence time showing favourable values. The optimal ranges for temperature, pH, and dissolved oxygen are determined to be between 23.56 to 29.11 ⁰C, 9.03 to 9.58, and 6.57 to 6.89 mgO2/L, respectively. Under the conditions of 15% CO2and PFD of 300µmol/ (m2.s), the specific growth rates reach 0.36±0.004 /day and maximum biomass concentration attains 1.52±0.03kg/m3. Notably, the PFD of 300µmol/ (m2.s) yields the highest conversion of carbon into biomass, ranging from 1.09×10-02kg to 1.26×10-02kg, representing the yield of 31 to 83% in each CO2injection treatment.