97 results on '"Mayakrishnan, Vijayakumar"'
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2. Serratia rubidaea SNAU02-mediated biosurfactant production from cashew apple bagasse: A promising biotechnological strategy for environmental sustainability
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Parthasarathi, Rengasamy, Mayakrishnan, Vijayakumar, Natesan, Vijayakumar, Akash, Krishnamoorthi, Poonguzhali, Pandurangan, Ramamoorthy, Kavitha, Kannappan, Priya, Soundharrajan, Ilavenil, Aljowaie, Reem M., Musaed Almutairi, Saeedah, and Ki Choon, Choi
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- 2024
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3. In silico identification of potential inhibitors against main protease of SARS-CoV-2 6LU7 from Andrographis panniculata via molecular docking, binding energy calculations and molecular dynamics simulation studies
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Mayakrishnan Vijayakumar, Balakarthikeyan Janani, Priya Kannappan, Senthil Renganathan, Sameer Al-Ghamdi, Mohammed Alsaidan, Mohamed A. Abdelaziz, Abubucker Peer Mohideen, Mohammad Shahid, and Thiyagarajan Ramesh
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Corona ,SARS-CoV-2 ,COVID-19 (6LU7) ,Natural compounds ,Diterpenoids ,Andrographolide ,Biology (General) ,QH301-705.5 - Abstract
Background: The ongoing global outbreak of new corona virus (SARS-CoV-2) has been recognized as global public health concern since it causes high morbidity and mortality every day. Due to the rapid spreading and re-emerging, we need to find a potent drug against SARS-CoV-2. Synthetic drugs, such as hydroxychloroquine, remdisivir have paid more attention and the effects of these drugs are still under investigation, due to their severe side effects. Therefore, the aim of the present study was performed to identify the potential inhibitor against main protease SARS-CoV-2 6LU7. Objective: In this study, RO5, ADME properties, molecular dynamic simulations and free binding energy prediction were mainly investigated. Results: The molecular docking study findings revealed that andrographolide had higher binding affinity among the selected natural diterpenoids compared to co-crystal native ligand inhibitor N3. The persistent inhibition of Ki for diterpenoids was analogous. Furthermore, the simulations of molecular dynamics and free binding energy findings have shown that andrographolide possesses a large amount of dynamic properties such as stability, flexibility and binding energy. Conclusion: In conclusion, findings of the current study suggest that selected diterpenoids were predicted to be the significant phytonutrient-based inhibitor against SARS-CoV-2 6LU7 (Mpro). However, preclinical and clinical trials are needed for the further scientific validation before use.
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- 2022
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4. Chemical Composition Analysis and Assessment of Antioxidant and Anti-Inflammatory Activities of Crude Extract of Flueggea leucopyrus on Carrageenan-Induced Paw Edema in Wistar Albino Rats.
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Mayakrishnan, Vijayakumar, Thirupathi, Anand, Ramamoorthy, Kavitha, Annadurai, Kaliappan, Prakasam, Radha, Gu, Yaodong, Kim, Choon Young, Ramasamy, Mahadevi, Karimpanchola, Habeebmon, Kannappan, Priya, Vijayakumar, Natesan, Venkatesan Kumari, Bhuvaneshwari, and Singaravelu, Anand
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FOURIER transform infrared spectroscopy ,CHEMICAL properties ,ANALYTICAL chemistry ,LABORATORY rats ,GAS chromatography/Mass spectrometry (GC-MS) ,ANTI-inflammatory agents - Abstract
A member of the Phyllanthaceae family, Flueggea leucopyrus is a well-known plant in the tribal areas of Sri Lanka, India's Shaurastra region, Australia, and Malaysia. This study provides information about Flueggea leucopyrus, a plant with a wide range of therapeutic uses in India. Different extracts from the leaves and roots of Flueggea leucopyrus were evaluated for their physical and chemical properties, preliminary phytochemical parameters, and pharmacological activities in the current study, followed by their fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC–MS), antioxidant, and anti-inflammatory properties. The aqueous extract of Flueggea leucopyrus leaves and roots have more different phytochemical elements than other solvent extracts, according to physico-chemical tests and phytochemical screening. As a result, the FT-IR, GC–MS, antioxidant, and anti-inflammatory activities of an aqueous extract were tested. Studies on hind paw edemas caused by carrageenan in albino rats examined the mean increase in paw volume and the percentage inhibition in paw volume at various time points following the injection of carrageenan (1% w/v). In comparison to the norm, these inhibitions were statistically significant (p < 0.001). The aqueous extract of Flueggea leucopyrus leaves and roots have both antioxidative and anti-inflammatory activities, indicating that it has the potential to be used in the formulation of antioxidant and anti-inflammatory medications in the future. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Investigation of phytochemical profile and in vivo anti-proliferative effect of Laetiporus versisporus (Lloyd) Imazeki mushroom against diethylnitrosamine-induced hepatocellular carcinoma
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Gunasekaran, Shoba, Mayakrishnan, Vijayakumar, Al-Ghamdi, Sameer, Alsaidan, Mohammed, Geddawy, Ayman, Abdelaziz, Mohamed A, Mohideen, Abubucker Peer, Bahakim, Nasraddin Othman, Ramesh, Thiyagarajan, and Ayyakannu, Usha Raja Nanthini
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- 2021
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6. A network-based pharmacological investigation to identify the mechanistic regulatory pathway of andrographolide against colorectal cancer
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Balakarthikeyan Janani, Mayakrishnan Vijayakumar, Kannappan Priya, Jin Hee Kim, Ayman Geddawy, Mohammad Shahid, Mahmoud H. El-Bidawy, Sameer Al-Ghamdi, Mohammed Alsaidan, Mohammad Hassan Abdelzaher, Abubucker Peer Mohideen, and Thiyagarajan Ramesh
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colorectal cancer ,molecular target ,network pharmacology ,protein-protein interaction ,gene ontology ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Traditional cancer treatments have posed numerous obstacles, including toxicity, multiple drug resistance, and financial cost. On the contrary, bioactive phytochemicals used in complementary alternative medicine have recently increased attention due to their potential to modulate a wide range of molecular mechanisms with a less toxic effect. Therefore, we investigated the potential regulatory mechanisms of andrographolide to treat colorectal cancer (CRC) using a network pharmacology approach. Target genes of andrographolide were retrieved from public databases (PharmMapper, Swiss target prediction, Targetnet, STITCH, and SuperPred), while targets related to CRC were retrieved from disease databases (Genecards and DisGeNet) and expression datasets (GSE32323 and GSE8671) were retrieved from gene expression omnibus (GEO). Protein-protein interaction networks (PPI) were generated using STRING and Cytoscape, and hub genes were identified by topology analysis and MCODE. Annotation of target proteins was performed using Gene Ontology (GO) database DAVID and signaling pathway enrichment analysis using the Kyoto Encyclopedia and Genome Database (KEGG). Survival and molecular docking analysis for the hub genes revealed three genes (PDGFRA, PTGS2, and MMP9) were involved in the overall survival of CRC patients, and the top three genes with the lowest binding energy include PDGFRA, MET, and MAPK1. MET gene upregulation and PDGFRA and PTGS2 gene downregulation are associated with the survival of CRC patients, as revealed by box plots and correlation analysis. In conclusion, this study has provided the first scientific evidence to support the use of andrographolide to inhibit cellular proliferation, migration, and growth, and induce apoptosis by targeting the hub genes (PDGFRA, PTGS2, MMP9, MAPK1, and MET) involved in CRC migration and invasion.
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- 2022
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7. Role of Corn Peptide Powder in Lipopolysaccharide-Induced Inflammatory Responses in 3T3-L1 Adipocytes.
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Mayakrishnan, Vijayakumar, Lee, Dae-Hee, Kim, Kee-Hong, and Kim, Choon Young
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Corn peptide (CP) is a short, naturally occurring, and physiologically active peptide generated from corn-protease-catalyzed hydrolysis. CP plays a role in preventing obesity-related disorders, but its impact on reducing inflammation is unknown. Hence, this study examined the possible protective effects of corn peptide powder (CPP) against the harmful effects of lipopolysaccharide (LPS), with a particular emphasis on reducing oxidative damage and inflammation in adipocytes. Hence, mature 3T3-L1 adipocytes underwent exposure to 10 ng/mL LPS, with or without CPP (10 and 20 μg/mL). LPS stimulation increased reactive oxygen species and superoxide anion generation. However, this effect was reduced in a dose-dependent manner by pretreatment with CPP. CPP treatment elevated the mRNA expressions of the antioxidant enzymes manganese superoxide dismutase (mnSOD) and glutathione peroxidase 1 (Gpx1) while reducing the mRNA expressions of the cytosolic reactive oxygen species indicators p40 and p67 (NADPH oxidase 2). In addition, CPP inhibited the monocyte chemoattractant protein-1, tumor necrosis factor-alpha, Toll-like receptor 4, and nuclear factor kappa B mRNA expressions induced by LPS. These findings demonstrate that CPP may ameliorate adipocyte dysfunction by suppressing oxidative damage and inflammatory responses through a new mechanism known as Toll-like receptor 4/nuclear factor kappa B-mediated signaling. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Effects of quercetin on ultrafine petrol exhaust nanoparticles induced DNA damage, oxidative stress and inflammation in different sections of rat brain
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Mohan Durga, Mayakrishnan Vijayakumar, Kannappan Priya, Shenbaga Vidhya Kanagarajan, Balakrishnan Brindha Banu, Victoriya Salomi Michael Abraham, Thiyagarajan Devasena, Mohamed A. Abdelaziz, Abubucker Peer Mohideen, Nasraddin Othman Bahakim, Mohammad Shahid, Shaban Ragab Ibrahim, and Thiyagarajan Ramesh
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Petrol exhaust particles ,Alzheimer’s disease ,Brain ,Neuroinflammation ,Phytochemicals ,Air pollution ,Science (General) ,Q1-390 - Abstract
The major constituent of air pollution is petrol exhaust a complex mixture of particles, gases and chemicals. The aim of the current research was to evaluate whether ultrafine petrol exhaust nanoparticles (PENPs), the particle component of exhaust from petrol engines can induce neurotoxicity in rats. We administered rats with repeated doses of PENPs (90 μg/rat and 180 μg/rat for 6 days (every second day) intratracheally (i.t.). This was followed by the evaluation of several neurotoxicity parameters in various sections of rat brain. PENP exposure caused surge in levels of inflammatory mediators such as reactive oxygen species (ROS) and neurodegenerative disorder indicators like amyloid beta 42 (Aβ42) levels in rat brain. Each section of the brain responded differently upon PENP exposure. Prior treatment with quercetin (60 mg/kg b.wt) inhibited elevation in the aforementioned parameters. Hence, PENP exposure was closely linked to neurotoxicity and the neuroprotective capacity of quercetin was also proved.
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- 2022
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9. EGFR-Based Targeted Therapy for Colorectal Cancer—Promises and Challenges
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Balakarthikeyan Janani, Mayakrishnan Vijayakumar, Kannappan Priya, Jin Hee Kim, D. S. Prabakaran, Mohammad Shahid, Sameer Al-Ghamdi, Mohammed Alsaidan, Nasraddin Othman Bahakim, Mohammad Hassan Abdelzaher, and Thiyagarajan Ramesh
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colorectal cancer ,EGFR ,nanocarriers ,nanomedicine ,cetuximab ,Medicine - Abstract
Colorectal carcinoma (CRC) is the most lethal and common form of cancer in the world. It was responsible for almost 881,000 cancer deaths in 2018. Approximately 25% of cases are diagnosed at advanced stages with metastasis—this poses challenges for effective surgical control and future tumor-related mortality. There are numerous diagnostic methods that can be used to reduce the risk of colorectal carcinoma. Among these, targeted nanotherapy aims to eliminate the tumor and any metastasis. Active targeting can increase the effectiveness and quantity of drugs delivered to the target site. Antibodies that target overexpressed receptors on cell surfaces and indicators are coupled with drug-loaded carriers. The major target receptors of chemotherapeutic drugs delivery include VEGFR, EGFR, FGFR, HER2, and TGF. On account of its major and diverse roles in cancer, it is important to target EGFR in particular for better tumor selection, as EGFR is overexpressed in 25 to 82% of colorectal carcinoma cases. The EGFR monoclonal immunoglobulins cetuximab/panitumumab can thus be used to treat colorectal cancer. This review examines carriers that contain cetuximab-conjugated therapeutic drugs as well as their efficacy in anticancer activities.
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- 2022
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10. Effects of supplementing Barodon, Bacillus subtilis, and Ampbio on growth performance, biochemical metabolites, and hormone levels in Korean native heifers
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Kim, Tae-Il, Lim, Dong-Hyun, Jang, Sun-Sik, Kim, Sang-Bum, Park, Seong-Min, Park, Ji-Hoo, Ki, Kwang-Seok, and Mayakrishnan, Vijayakumar
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- 2018
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11. The effect of lactation number, stage, length, and milking frequency on milk yield in Korean Holstein dairy cows using automatic milking system
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Mayakrishnan Vijayakumar, Ji Hoo Park, Kwang Seok Ki, Dong Hyun Lim, Sang Bum Kim, Seong Min Park, Ha Yeon Jeong, Beom Young Park, and Tae Il Kim
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Milk Yield ,Lactation Number ,Lactation Length ,Milking Frequency ,Automatic Milking System (AMS) ,Korean Holstein Dairy Cows ,Animal culture ,SF1-1100 ,Animal biochemistry ,QP501-801 - Abstract
Objective The aim of the current study was to describe the relationship between milk yield and lactation number, stage, length and milking frequency in Korean Holstein dairy cows using an automatic milking system (AMS). Methods The original data set consisted of observations from April to October 2016 of 780 Holstein cows, with a total of 10,751 milkings. Each time a cow was milked by an AMS during the 24 h, the AMS management system recorded identification numbers of the AMS unit, the cow being milking, date and time of the milking, and milk yield (kg) as measured by the milk meters installed on each AMS unit, date and time of the lactation, lactation stage, milking frequency (NoM). Lactation stage is defined as the number of days milking per cows per lactation. Milk yield was calculated per udder quarter in the AMS and was added to 1 record per cow and trait for each milking. Milking frequency was measured the number of milkings per cow per 24 hour. Results From the study results, a significant relationship was found between the milk yield and lactation number (p201 days) of cows. Also, milking frequency had a significant influence on milk yield (p
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- 2017
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12. In silico identification of potential inhibitors against main protease of SARS-CoV-2 6LU7 from Andrographis panniculata via molecular docking, binding energy calculations and molecular dynamics simulation studies
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Mohammed Alsaidan, Balakarthikeyan Janani, Mohammad Shahid, Mayakrishnan Vijayakumar, Priya Kannappan, Senthil Renganathan, Abubucker Peer Mohideen, Sameer Al-Ghamdi, Thiyagarajan Ramesh, and Mohamed A. Abdelaziz
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Drug ,food.ingredient ,QH301-705.5 ,medicine.medical_treatment ,In silico ,media_common.quotation_subject ,Andrographolide ,Binding energy ,Computational biology ,CNS, central nervous system ,ACE 2, Angiotensin Converting Enzyme ,chemistry.chemical_compound ,food ,natural compounds ,medicine ,Biology (General) ,COVID-19, coronavirus disease 2019 ,ADME ,media_common ,SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 ,Protease ,SARS-CoV-2 ,Chemistry ,andrographolide ,diterpenoids ,Ligand (biochemistry) ,molecular dynamic simulations ,Andrographis ,Mpro, Main protease ,COVID-19 (6LU7) ,Corona ,Original Article ,General Agricultural and Biological Sciences - Abstract
Background The ongoing global outbreak of new corona virus (SARS-CoV-2) has been recognized as global public health concern since it causes high morbidity and mortality every day. Due to the rapid spreading and re-emerging, we need to find a potent drug against SARS-CoV-2. Synthetic drugs, such as hydroxychloroquine, remdisivir have paid more attention and the effects of these drugs are still under investigation, due to their severe side effects. Therefore, the aim of the present study was performed to identify the potential inhibitor against main protease SARS-CoV-2 6LU7. Objective In this study, RO5, ADME properties, molecular dynamic simulations and free binding energy prediction were mainly investigated. Results The molecular docking study findings revealed that andrographolide had higher binding affinity among the selected natural diterpenoids compared to co-crystal native ligand inhibitor N3. The persistent inhibition of Ki for diterpenoids was analogous. Furthermore, the simulations of molecular dynamics and free binding energy findings have shown that andrographolide possesses a large amount of dynamic properties such as stability, flexibility and binding energy. Conclusion In conclusion, findings of the current study suggest that selected diterpenoids were predicted to be the significant phytonutrient-based inhibitor against SARS-CoV-2 6LU7 (Mpro). However, preclinical and clinical trials are needed for the further scientific validation before use.
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- 2022
13. Hepatoprotective mechanism of lycorine against carbon tetrachloride induced toxicity in swiss albino mice – A proteomic approach
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Soundarrajan Ilavenil, Dhanaraj Karthik, Mariadhas Valan Arasu, Mayakrishnan Vijayakumar, Srisesharam Srigopalram, Selvaraj Arokiyaraj, Sivanesan Ravikumar, and Ki Choon Choi
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Lycorine ,CCl4 ,Oxidative stress ,2D gel ,MALDI-TOF-MS ,ATP synthase ,Regucalcin ,HSP 60 ,Medicine - Abstract
Objective: To investigate the differential of protein expression in CCl4 induced mice treated with lycorine. Methods: The present study was carried out to identify the differentially expressed protein in carbon tetrachloride (CCl4) induced oxidative stress mice treated with lycorine (5 mg/kg. bw) using 2D gel and MALDI-TOF. Results: We observed many kinds of differentially expressed protein in experimental liver. Among these, three are much differently expressed protein which is identified as ATP synthase, regucalcin and HSP60; these proteins are involved in the ATP synthesis, calcium regulation and rescue the integrity cellular proteins respectively. Conclusions: This investigation provided a molecular mechanism of the lycorine during CCl4 induced oxidative stress in mice liver.
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- 2015
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14. Bacterial cancer therapy: A turning point for new paradigms
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Mayakrishnan, Vijayakumar, primary, Kannappan, Priya, additional, Tharmalingam, Nagendran, additional, Bose, Rajendran J C, additional, Madheswaran, Thiagarajan, additional, and Ramasamy, Mohankandhasamy, additional
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- 2022
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15. Trigonelline attenuates the adipocyte differentiation and lipid accumulation in 3T3-L1 cells
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Ilavenil, Soundharrajan, Arasu, Mariadhas Valan, Lee, Jeong-Chae, Kim, Da Hye, Roh, Sang Gun, Park, Hyung Su, Choi, Gi Jun, Mayakrishnan, Vijayakumar, and Choi, Ki Choon
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- 2014
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16. Preparation of Nanoemulsions of Vitamin A and C by Microfluidization: Efficacy on the Expression Pattern of Milk-Specific Proteins in MAC-T Cells
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Tae-Il Kim, Tae-Gyun Kim, Dong-Hyun Lim, Sang-Bum Kim, Seong-Min Park, Tai-Young Hur, Kwang-Seok Ki, Eung-Gi Kwon, Mayakrishnan Vijayakumar, and Young-Jun Kim
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nanoemulsion ,microfluidization ,vitamins ,casein ,Organic chemistry ,QD241-441 - Abstract
In this study, we prepared stabilized vitamin A and C nanoemulsions, and investigated their efficacy on milk-specific proteins in bovine mammary epithelial cells (MAC-T). Emulsions of vitamin A (vit-A) and C (vit-C) were prepared using Lipoid S 75 and microfluidization. The particle size and polydispersity index (PDI) of nanoemulsified vit-A and vit-C were studied. The cytotoxic effect of nanoemulsion-free and nanoemulsified vit-A and vit-C was determined by an MTT assay. In addition, the efficacy of nanoemulsified vit-A and vit-C on the in vitro expression pattern of milk-specific proteins in MAC-T cells was investigated by quantitative RT-PCR. The results showed that the efficacies of stabilized nanoemulsions of vit-A and vit-C were 100% and 92.7%, respectively. The particle sizes were around 475.7 and 225.4 nm, and the zeta potentials were around −33.5 and −21.3 mV, respectively. The expression changes of αs2-, β- and κ-casein were higher in the presence of a stabilized nanoemulsion of vit-A, compared with nanoemulsion-free vit-A. Furthermore, the expression changes of αs2- and β-casein were lower and that of κ-casein was higher in the presence of a stabilized nanoemulsion of vit-C, compared with nanoemulsion-free vit-C. Thus, our findings demonstrate the efficacy of nanoemulsified vit-A and vit-C in changing the expression of milk-specific proteins in MAC-T cells.
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- 2019
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17. Free radical scavenging potential of Lagenaria siceraria (Molina) Standl fruits extract
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Mayakrishnan, Vijayakumar, Veluswamy, Selvi, Sundaram, Krishnakumari Shanmuga, Kannappan, Priya, and Abdullah, Noorlidah
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- 2013
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18. Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells
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Mayakrishnan Vijayakumar, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Soundharrajan Ilavenil, Hak-Jae Kim, Kannappan Priya, Balakarthikeyan Janani, Thiyagarajan Ramesh, Young-Ock Kim, and Vadanasundari Vedarethinam
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Carcinoma, Hepatocellular ,Silver ,Poly ADP ribose polymerase ,Metal Nanoparticles ,Antineoplastic Agents ,Apoptosis ,Chitin ,02 engineering and technology ,Biochemistry ,Silver nanoparticle ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,Ultraviolet visible spectroscopy ,Animal Shells ,Structural Biology ,Crustacea ,medicine ,Animals ,Humans ,Cytotoxicity ,Molecular Biology ,Cell Proliferation ,030304 developmental biology ,0303 health sciences ,medicine.diagnostic_test ,Caspase 3 ,Chemistry ,Liver Neoplasms ,Hep G2 Cells ,General Medicine ,021001 nanoscience & nanotechnology ,Caspase 9 ,Gene Expression Regulation, Neoplastic ,Proto-Oncogene Proteins c-bcl-2 ,Cancer cell ,0210 nano-technology ,Nuclear chemistry - Abstract
In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions. Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells. Ultraviolet visible spectroscopy (UV–Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm. FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites. The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture. Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5 μg/ml. The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow cytometry. The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs. These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.
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- 2020
19. Models Describing Growth Characteristics of Holstein Dairy Cows Raised in Korea
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Dong Hyun Lim, Heechul Choi, M. Alam, Mayakrishnan Vijayakumar, Hyun-Jeong Lee, Seong-Min Park, Yunho Choy, Tae Il Kim, and Kwang Seok Ki
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Animal science ,Rump ,Withers ,Gompertz function ,Linear regression ,Covariate ,Growth curve (biology) ,Girth (geometry) ,Regression ,Mathematics - Abstract
The objective of the present study was to determine the best model to describe and quantify the changes in live body weight, height at withers, height at rump, body length and chest girth of Holstein cows raised under Korean feeding conditions for 50 months. The five standard growth models namely polynomial linear regression models, regression of growth variables on the first and second-order of ages in days (model 1) and regression of growth variables on age covariates from first to the third-order (model 2) as well as non-linear models were fitted and evaluated for representing growth pattern of Holstein cows raised in Korean feeding circumstances. Nonlinear models fitted were three exponential growth curve models; Brody, Gompertz, and von Bertalanffy functional models. For this purpose, a total of 22 Holstein cows raised in Korea used in the period from April 2016 to May 2020. Each model fitted to monthly growth curve records of dairy cows by using PROC NLIN procedure in SAS program. On the basis of the results, nonlinear models showed the lower root mean square of error (RMSE) for live body weight, height at withers, height at rump, body length and chest girth (12.22, 1.95, 1.55, 4.04, 2.06) with higher correlation coefficiency (R2) values for live body weight, height at withers, height at rump, body length and chest girth (0.99, 0.99, 0.99, 1.00, 1.00). Overall, the evaluation of the different growth models indicated that the Gompertz model used in the study seemed to be the most appropriate one for standard growth of Holstein cows raised under Korean feeding system.
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- 2020
20. Modulation of Renin-Angiotensin System by Aqueous Extract of Large Cardamom: In vitro and in silico Studies
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P Uma Devi, S.K. Kanthlal, Kavyanjana R. Nair, Mayakrishnan Vijayakumar, V.S. Arya, and Bindhu Paul Prasanth
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Pharmacology ,Aqueous extract ,High prevalence ,business.industry ,In silico ,Vasodilation ,Plant Science ,Toxicology ,complex mixtures ,Agricultural and Biological Sciences (miscellaneous) ,In vitro ,Complementary and alternative medicine ,Drug Discovery ,Renin–angiotensin system ,Medicine ,business - Abstract
Hypertension is a growing public health concern due to its high prevalence all around the globe. Spices and herbs are known to possess antihypertensive activities. Hence, we evaluated the therapeut...
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- 2020
21. Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells
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Balakarthikeyan Janani, Mayakrishnan Vijayakumar, and Kannappan Priya
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Carcinoma, Hepatocellular ,Silver ,Biocompatibility ,Cell Survival ,Metal Nanoparticles ,Nanoparticle ,Apoptosis ,DNA Fragmentation ,02 engineering and technology ,engineering.material ,Biochemistry ,Silver nanoparticle ,Chitosan ,03 medical and health sciences ,chemistry.chemical_compound ,Structural Biology ,Humans ,Cytotoxicity ,Molecular Biology ,030304 developmental biology ,Gel electrophoresis ,0303 health sciences ,Spectrum Analysis ,Liver Neoplasms ,Hep G2 Cells ,General Medicine ,021001 nanoscience & nanotechnology ,Anti-Bacterial Agents ,chemistry ,engineering ,Trypan blue ,Biopolymer ,0210 nano-technology ,Nuclear chemistry - Abstract
The biopolymer chitosan is currently in widespread use because of its nontoxicity, biocompatibility and biodegradability. Therefore, in this study, chitosan extracted from shrimp shells was used to synthesise biogenic silver nanoparticles (AgNPs). UV–visible spectrophotometry of reduced silver nanoparticles in the colloidal solution showed a single peak at 400 nm, confirming the formation of AgNPs. The presence of biomolecules responsible for reducing and capping the biogenic AgNPs was confirmed by FTIR. Surface morphology of the biosynthesised AgNPs was characterised using SEM, and TEM analysis showed the formation of spherical shapes 17–50 nm. The presence of elemental silver in the synthesised biogenic AgNPs was confirmed using EDX and the crystalline structure characterised by XRD. Cytotoxicity of biogenic AgNPs was determined using MTT and the trypan blue exclusion assay. Morphological changes in HepG2 cells were detected by analysis of the DNA ladder pattern via gel electrophoresis, and the IC50 of HepG2 cell inhibition by AgNPs was 48 μg/ml. The upregulated caspase 3 and 9 protein expression results confirmed cell death via apoptosis. In conclusion, chitosan has the ability to synthesise AgNPs with in vitro apoptotic activities.
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- 2020
22. Effect of Barn or Grazing on Biochemical Indices in Prepartum, and Milk Composition in Postpartum of Dairy Cows
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Kwang-Seok Ki, Sang Bum Kim, Hee-Chul Choi, Seong-Min Park, Ji-Hoo Park, Dong-Hyun Lim, Tae-Il Kim, Dong-Hyeon Kim, Jeong Sung Jung, Mayakrishnan Vijayakumar, and Hyun-Jeong Lee
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animal diseases ,food and beverages ,Biology ,Feed conversion ratio ,Palmitic acid ,chemistry.chemical_compound ,Oleic acid ,Animal science ,chemistry ,Saturated fatty acid ,Grazing ,Arachidonic acid ,Composition (visual arts) ,reproductive and urinary physiology ,Barn (unit) - Abstract
The present study was designed to determine the effect of barn or cycle of grazing on changes of biochemical metabolites in prepartum and changes of milk composition in postpartum of dairy cows. For this purpose, a total of sixteen 25 months old Holstein primiparous dairy cows were allocated in two groups (n=8) with an average body weight of 571.61 ± 35.30 kg (Barn) and 578.10 ± 39.20 kg (Grazing). The study was conducted from June 2018 to October 2018. Results revealed that barn raised dairy cows had a higher increase in their serum albumin and calcium level on day 14 prepartum. However, the level of palmitic acid, saturated fatty acid increased significantly, and the level of fat, oleic acid, γ-linoleic acid, arachidonic acid and unsaturated fatty acids decreased significantly in barn raised dairy cow’s milk on day 14 postpartum. There were no significant differences observed with respect to all other biochemical metabolites, fatty acids and minerals between barn raised and cycle grazing dairy cows during prepartum and postpartum. Our study results could serve to a better understanding of barn raised cow with respect to changes of biochemical metabolites in prepartum and changes of milk composition, fatty acids and minerals content in grazing dairy cows in postpartum for estimating their physiological status.
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- 2019
23. Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure
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Soundharrajan Ilavenil, Hyung Soo Park, Mayakrishnan Vijayakumar, Mariadhas Valan Arasu, Da Hye Kim, Sivanesan Ravikumar, and Ki Choon Choi
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Technology ,Medicine ,Science - Abstract
The aim of the study was to isolate and characterize the lactic acid bacteria (LAB) from animal manure. Among the thirty LAB strains, four strains, namely, KCC-25, KCC-26, KCC-27, and KCC-28, showed good cell growth and antifungal activity and were selected for further characterization. Biochemical and physiology properties of strains confirmed that the strains are related to the Lactobacillus sp.; further, the 16S rRNA sequencing confirmed 99.99% sequence similarity towards Lactobacillus plantarum. The strains exhibited susceptibility against commonly used antibiotics with negative hemolytic property. Strains KCC-25, KCC-26, KCC-27, and KCC-28 showed strong antifungal activity against Aspergillus fumigatus, Penicillium chrysogenum, Penicillium roqueforti, Botrytis elliptica, and Fusarium oxysporum, respectively. Fermentation studies noted that the strains were able to produce significant amount of lactic, acetic, and succinic acids. Further, the production of extracellular proteolytic and glycolytic enzymes, survival under low pH, bile salts, and gastric juice together with positive bile salt hydrolase (Bsh) activity, cholesterol lowering, cell surface hydrophobicity, and aggregation properties were the strains advantages. Thus, KCC-25, KCC-26, KCC-27, and KCC-28 could have the survival ability in the harsh condition of the digestive system in the gastrointestinal tract. In conclusion, novel L. plantarum KCC-25, KCC-26, KCC-27, and KCC-28 could be considered as potential antimicrobial probiotic strains.
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- 2015
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24. Effect of Short-distance Walking Activity on Milk Production and Metabolic Status of Lactating Dairy Cows
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Mayakrishnan Vijayakumar, Kim Sangbeom, Im Donghyeon, Jeong, Ha-Yeon, Kim Hyeonjong, Park Seong-Min, Lee Jihwan, Lim Hyeonju, Park Jihu, Kim Taeil, and Ha Seungmin
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Animal science ,Walking (activity) ,Biology ,Milk production ,Short distance - Published
- 2018
25. The effect of seasonal thermal stress on milk production and milk compositions of Korean Holstein and Jersey cows
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Lim, Dong-Hyun, primary, Mayakrishnan, Vijayakumar, additional, Ki, Kwang-Seok, additional, Kim, Younghoon, additional, and Kim, Tae-Il, additional
- Published
- 2021
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26. Effects of Replacing Corn with Hydroponically Sprouted Barley on the Growth Performance and Blood Metabolite Status of Holstein Dairy Heifers
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Kim, Tae-Il, primary, Lim, Dong-Hyun, additional, Lee, Hyun-Jeong, additional, Park, Seong-Min, additional, Kim, Yoon-Jeong, additional, Choi, Hee-Chul, additional, Park, Jong-Ho, additional, Kim, Sam-Churl, additional, Ki, Kwang-Seok, additional, and Mayakrishnan, Vijayakumar, additional
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- 2020
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27. A comparative study on milk composition of Jersey and Holstein dairy cows during the early lactation
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Lim, Dong-Hyun, primary, Mayakrishnan, Vijayakumar, additional, Lee, Hyun-Jeong, additional, Ki, Kwang-Seok, additional, Kim, Tae-Il, additional, and Kim, Younghoon, additional
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- 2020
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28. Effectiveness of using staircase as a lifestyle modification among sedentary workers of a municipal corporation in improving fitness level- an interventional study
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T Manivannan, S Vinothkumar, Mayakrishnan Vijayakumar, and K. Caroline Priya
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Lifestyle modification ,Municipal corporation ,Environmental health ,Business ,human activities - Abstract
Background: Sedentary lifestyle is one of the important risk factors for cardiovascular diseases which is the leading cause of death globally. Workplace initiatives like promotion of staircase use are one among many simple measures to overcome the harmful effect of a sedentary workplace. Hence this study was conducted to assess effectiveness of ‘using staircase’ as a lifestyle modification among sedentary workers of a municipal corporation in improving fitness level.Methods: An interventional study was conducted over a period of 8 weeks among workers of a municipal corporation office. A semi structured questionnaire including socio demographic and morbidity details was administered. sitting-rising test (SRT) was administered before and after 8 weeks staircase use intervention to assess fitness levels among study participants.Results: The mean SRT score of 172 study population at baseline was 8.79 and 115 (67%) recorded above it. 3.5-5. Younger age was associated with higher SRT score and those with history of smoking, alcohol consumption, hypertension, diabetes, hyperlipidemia and higher BMI had lower SRT scores than their counterparts. Mean value of SRT significantly improved to 9.015 following intervention.Conclusions: Fitness level assessed using SRT score among office staff showed that people with higher age and higher BMI have low SRT score than their counterparts. This study has shown that staircase usage can be prescribed as effective method to improve fitness level at sedentary workplace.
- Published
- 2021
29. Moringa concanensis Nimmo extracts ameliorates hyperglycemia-mediated oxidative stress and upregulates PPARγ and GLUT4 gene expression in liver and pancreas of streptozotocin-nicotinamide induced diabetic rats
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Balakrishnan, Brindha Banu, primary, Krishnasamy, Kalaivani, additional, Mayakrishnan, Vijayakumar, additional, and Selvaraj, Arokiyaraj, additional
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- 2019
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30. The effect of nanoemulsified methionine and cysteine on the in vitro expression of casein in bovine mammary epithelial cells
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Kim, Tae-Il, primary, Kim, Tae-Gyun, additional, Lim, Dong-Hyun, additional, Kim, Sang-Bum, additional, Park, Seong-Min, additional, Lim, Hyun-Joo, additional, Kim, Hyun-Jong, additional, Ki, Kwang-Seok, additional, Kwon, Eung-Gi, additional, Kim, Young-Jun, additional, and Mayakrishnan, Vijayakumar, additional
- Published
- 2019
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31. Effect of Lactic Acid Bacteria Inoculation on Fermentation Characteristics of Whole Crop Barley Silage
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Kyung Dong Lee, Soundharrajan Ilavenil, Mayakrishnan Vijayakumar, Ki Choon Choi, Hyung Soo Park, and Srisesharam Srigopalram
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biology ,Silage ,Chemistry ,food and beverages ,biology.organism_classification ,Lactic acid ,Butyric acid ,Acetic acid ,chemistry.chemical_compound ,Neutral Detergent Fiber ,Agronomy ,Dry matter ,Fermentation ,Food science ,Lactobacillus plantarum - Abstract
An experiment was carried out to determine the homofermentative activity of Lactobacillus plantarum KCC-10 and KCC-19 on the ensiling of whole crop barley (WCB). The crude protein in the silages was slightly higher in the KCC-10 and KCC-19 treatments compared to the control, but there was no significant difference between the two inoculant-treated silages. Nutrient parameters such as acid detergent fiber, neutral detergent fiber and in vitro dry matter digestibility in L. plantarum KCC-10 and KCC-19 treated silages did not differ from those in the control silage. The lactic acid content increased in KCC-10 and KCC-19 treated silage when compared with the control silage but the contents of acetic acid and butyric acid produced in KCC-10 and KCC-19 treated silages were similar with the control silage. Further, the number of lactic acid bacteria (LAB) in KCC-10 treated silage demonstrated a significant increase when compared to the control. Especially, KCC-19 treated silage showed greater lactic acid bacterial growth potential. Other microbes such as yeast and fungi were not detected in KCC-10 and KCC-19 treated WCB silages. Hence, this study suggests that the addition of L. Plantarum KCC-10 and KCC-19 to the WCB silage can improve fermentation quality for the production of high-quality silage.
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- 2015
32. Addition of Novel Lactobacillus plantarum KCC-10 and KCC-19 to Improve Fermentation Quality and Characterization of Italian Ryegrass Silage
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Mayakrishnan Vijayakumar, Hyung Soo Park, Ki Choon Choi, Srisesharam Srigopalram, Kyung Dong Lee, and Soundharrajan Ilavenil
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biology ,Silage ,Gram-positive bacteria ,food and beverages ,biology.organism_classification ,Lactic acid ,chemistry.chemical_compound ,Neutral Detergent Fiber ,Biochemistry ,chemistry ,Dry matter ,Fermentation ,Food science ,Bacteria ,Lactobacillus plantarum - Abstract
An investigation was carried out to evaluate the potential role of Lactobacillus Plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of Italian ryegrass (IRG) silages. The physiochemical properties of IRG silage such as crude protein content, neutral detergent fiber, acid detergent fiber, total digestible nutrient and in vitro dry matter digestibility were not affected by KCC-10 and KCC-19. The pH of IRG silage in KCC-10 and KCC-19 treatments decreased compared to the control (p
- Published
- 2015
33. In-vitro assessment of the probiotic potential of Lactobacillus plantarum KCC-24 isolated from Italian rye-grass ( Lolium multiflorum ) forage
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Soundharrajan Ilavenil, Da Hye Kim, Kannappan Priya, Mariadhas Valan Arasu, Ki Choon Choi, and Mayakrishnan Vijayakumar
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Microbial Sensitivity Tests ,Microbiology ,Bacterial Adhesion ,law.invention ,Probiotic ,chemistry.chemical_compound ,law ,RNA, Ribosomal, 16S ,Antibiosis ,Fusarium oxysporum ,Lolium ,Microbial Viability ,biology ,Probiotics ,food and beverages ,Penicillium roqueforti ,Lolium multiflorum ,biology.organism_classification ,Penicillium chrysogenum ,Anti-Bacterial Agents ,Lactic acid ,Infectious Diseases ,chemistry ,Bacteria ,Lactobacillus plantarum - Abstract
The aim of the present study was to determine the probiotic potential of the lactic acid bacteria Lactobacillus plantarum KCC-24 (L. plantarum KCC-24), that was isolated and characterized from Italian ryegrass (Lolium multiflorum) forage. The following experiments were performed to assess the probiotic characteristics such as antifungal activity, antibiotic susceptibility, resistance to low pH, stimulated gastric juice and bile salts, proteolytic activity, auto-aggregation, cell surface hydrophobicity, and in vitro antioxidant property. The isolated L. plantarum KCC-24 exhibited significant antifungal activity against the various fungal strains of Aspergillus fumigatus (73.43%), Penicillium chrysogenum (59.04%), Penicillium roqueforti (56.67%), Botrytis elliptica (40.23%), Fusarium oxysporum (52.47%) and it was susceptible to numerous antibiotics, survived in low pH, was resistant to stimulated gastric juices and bile salts (0.3% w/v). Moreover, L. plantarum KCC-24 exhibited good proteolytic activity. In addition L. plantarum KCC-24 showed potent antioxidant and hydrogen peroxide resistant property. In conclusion, the isolated L. plantarum KCC-24 exhibited several characteristics to prove it's excellent as a potential probiotic candidate for developing quality food for ruminant animals and human.
- Published
- 2015
34. Assessment of probiotic, antifungal and cholesterol lowering properties of Pediococcus pentosaceus KCC-23 isolated from Italian ryegrass
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S. Ravikumar, Da Hye Kim, Mariadhas Valan Arasu, Mayakrishnan Vijayakumar, Soundharrajan Ilavenil, Ki Choon Choi, and Hyung Su Park
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0301 basic medicine ,Nutrition and Dietetics ,biology ,Inulin ,food and beverages ,biology.organism_classification ,Microbiology ,Genetically modified organism ,law.invention ,Aspergillus fumigatus ,Lactic acid ,03 medical and health sciences ,chemistry.chemical_compound ,Probiotic ,030104 developmental biology ,chemistry ,law ,Fusarium oxysporum ,Raffinose ,Agronomy and Crop Science ,Bacteria ,Food Science ,Biotechnology - Abstract
BACKGROUND Lactic acid bacteria (LAB) are important for the processing of various food products. Although genetically modified organisms have contributed to improvements in various food products, there are some limitations. Thus, the discovery of wild strains from natural sources must be considered as the most suitable approach for identifying new LAB. Therefore, we planned to isolate and characterise the LAB from Italian ryegrass forage and evaluate their biological potential. RESULTS A total of 28 strains were isolated and screened for their anti-fungal and probiotic properties. A single strain was selected due to its antifungal and probiotic efficiency. The strain was identified as Pediococcus pentosaceus KCC-23. The strain KCC-23 showed effective inhibition against Aspergillus fumigatus, Pencillium chrysogenum, Pencillium roqueforti, Botrytis elliptica and Fusarium oxysporum. Further, it survived low pH, and the presence of bile salts and gastric juice. It exhibited significant aggregation and hydrophobicity properties. The KCC-23 effectively assimilated cholesterol and had the ability to utilise pre-biotics such as raffinose and inulin. Finally, KCC-23 exhibited significant free radical scavenging activity. CONCLUSION P. pentosaceus KCC-23 showed effective anti-fungal, probiotic and anti-oxidant properties and would be a promising isolate for exploitation in the formulation of food for ruminants and humans. © 2015 Society of Chemical Industry
- Published
- 2015
35. MOESM4 of Novel Acinetobacter parvus HANDI 309 microbial biomass for the production of N-acetyl-β-d-glucosamine (GlcNAc) using swollen chitin substrate in submerged fermentation
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Kim, Tae, Ki, Kwang, Lim, Dong, Mayakrishnan Vijayakumar, Park, Seong, Choi, Sun, Kim, Ki, Im, Seok, and Park, Beom
- Abstract
Additional file 4: Figure S4. Effect of KNO3 of the culture medium on the extracellular chitinase activity.
- Published
- 2017
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36. Lactobacillus plantarum Improves the Nutritional Quality of Italian Ryegrass with Alfalfa Mediated Silage
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Hyung Soo Park, Ki Choon Choi, Mariadhas Valan Arasu, Min-Woong Jung, Young Cheol Lim, Mayakrishnan Vijayakumar, and Soundarrajan Ilavenil
- Subjects
biology ,Silage ,food and beverages ,biology.organism_classification ,Yeast ,Microbiology ,Lactic acid ,Butyric acid ,chemistry.chemical_compound ,Neutral Detergent Fiber ,chemistry ,Dry matter ,Fermentation ,Food science ,Lactobacillus plantarum - Abstract
The present study was planned to analyze the nutritional quality, microbial counts and fermentative acids in Italian ryegrass (IRG) 80% and alfalfa 20% (IRG-HV) mediated silage inoculated with lactic acid bacteria (LAB) as a probiotic strain for 3 months. Crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), total digestible nutrient (TDN) and In- vitro dry matter digestibility (IVDMD), lactic acid bacteria (LAB), yeast and fungi counts and fermentation metabolites such as lactic acid, acetic acid and butyric acids were analyzed. The result shows that the nutritional quality and metabolite profiles of silage were significantly improved with LAB. For microbial counts, LAB showed dominant followed by yeast as compared with control silage. The pH of the silage also reduced significantly when silage inoculated with LAB. The result confirmed that silage preparation using different crops with L. plantarum inoculation is most beneficial for the farmers.(
- Published
- 2014
37. Enhancement of Nutritional Quality of Italian Ryegrass Mediated Silage by Supplemented with Lactic Acid Bacteria and Chlorella
- Author
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Hyung Soo Park, Min-Woong Jung, Mayakrishnan Vijayakumar, Soundarrajan Ilavenil, Young Cheol Lim, Ji Hea Kim, Ki Choon Choi, and Mariadhas Valan Arasu
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biology ,Silage ,biology.organism_classification ,Lactic acid ,Butyric acid ,chemistry.chemical_compound ,Chlorella ,Neutral Detergent Fiber ,Acetic acid ,chemistry ,Biochemistry ,Dry matter ,Food science ,Bacteria - Abstract
The aim of present study was to improve the quality of silage using lactic acid bacteria (LAB) and chlorella as a supplement. Italian ryegrass (IRG) mediated silage was prepared with lactic acid bacteria (L. plantarum) and different concentration of chlorella. We analyzed the nutritional profiles such as crude protein (CP), acid detergent fiber (ADF) neutral detergent fiber (NDF), total digestible nutrient (TDN) and in-vitro dry matter digestibility (IVDMD), microbial counts and fermentative acids such as lactic acid, acetic acid and butyric acid in the control and experimental silage after three months. It shows increased crude protein content and also maintains the rest of nutritional values as compared with control silage. LAB inoculation with chlorella as supplementation slightly reduced the pH of the silage. In addition, it increased the fermentative acids production as compared with control silage and inhibits the undesired microbial growth especially fungi in the silage. Therefore, we suggest that LAB inoculation and chlorella supplementation to the IRG mediated silage could be improved the nutritional quality of the silage which is an intrinsic feature for the application in the preparation of animal feeds and functional foods.
- Published
- 2014
38. Cyathula prostrata: A potent source of anticancer agent against daltons ascites in Swiss albino mice
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K Priya, Mayakrishnan Vijayakumar, and S. Krishnakumari
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MTT ,Cell Survival ,Cell ,DNA Fragmentation ,Pharmacology ,medicine.disease_cause ,Ehrlich ascites carcinoma ,Mice ,Cell Line, Tumor ,Ascites ,medicine ,Animals ,Humans ,Cyathula prostrata ,MTT assay ,Carcinoma, Ehrlich Tumor ,Cancer ,Medicine(all) ,Amaranthaceae ,Plant Extracts ,business.industry ,Antitumor ,General Medicine ,Antineoplastic Agents, Phytogenic ,medicine.anatomical_structure ,DNA fragmentation ,Female ,Methotrexate ,medicine.symptom ,Carcinogenesis ,business ,medicine.drug - Abstract
Objective To evaluate the anticancer activity of the methanolic extract of Cyathula prostrata in Ehrlich ascites carcinoma (EAC)-bearing mice with methotrexate as positive control in the advanced stage of tumorigenesis. Methods EAC was induced in swiss albino mice by injecting 106 cell/mL of tumor cell suspension intraperitoneal. The methanolic extract of Cyathula prostrata effect on the tumor cell viability, DNA fragmentation and MTT assay were carried out. Results Methanolic extract attenuated percentage increased in the cell survival time when compared to control group. However, the effect was less than that of methotrexat. Methotrexat and the extracts reversed the tumor-induced alterations in DNA fragmentation and MTT assay. Conclusions The present study suggests that the methanol extract of Cyathula prostrata has significant anticancer activity and that is comparable to that of methotrexate.
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- 2013
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39. Production of chitinase from Escherichia fergusonii, chitosanase from Chryseobacterium indologenes, Comamonas koreensis and its application in N-acetylglucosamine production
- Author
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Kim, Tae Il, primary, Lim, Dong Hyun, additional, Baek, Kwang Soo, additional, Jang, Sun Sik, additional, Park, Beom Young, additional, and Mayakrishnan, Vijayakumar, additional
- Published
- 2018
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40. Effect of fermented total mixed rations on the growth performance, carcass and meat quality characteristics of Hanwoo steers
- Author
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Kim, Tae-Il, primary, Mayakrishnan, Vijayakumar, additional, Lim, Dong-Hyun, additional, Yeon, Jeong-Ha, additional, and Baek, Kwang-Soo, additional
- Published
- 2017
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41. Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica
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Mayakrishnan Vijayakumar, F.T. Nancy, Kannappan Priya, A. Noorlidah, and Abdul Bakrudeen Ali Ahmed
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Silver nitrate ,chemistry.chemical_compound ,chemistry ,Scanning electron microscope ,Reducing agent ,Nanoparticle ,Organic chemistry ,Antibacterial activity ,Hydrate ,Agronomy and Crop Science ,Silver nanoparticle ,Catalysis ,Nuclear chemistry - Abstract
a b s t r a c t Currently, there is an increasing commercial demand for nanoparticles due to their wide applicability in various markets, such as medicine, catalysis, electronics, chemistry and energy. In this report, a simple and eco-friendly chemical reaction for the synthesis of silver nanoparticles (AgNPs) from Artemisia nilagirica (Asteraceae) has been developed. Silver nitrate was used as the metal precursor and hydrazine hydrate as a reducing agent. Scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDX) were used to characterise the nanoparticles obtained from A. nilagirica. The morphology of the AgNPs was determined by SEM and the average diameter of the particles was determined as 70-90 nm. The EDX analysis of the nanoparticles dispersion, using a range of 2-4 keV, confirmed the presence of elemental silver, with no other impurity peaks detected. In addition, the characterised AgNPs has the potential for various medical and industrial applications. The results showed that microbial susceptibility to AgNPs is different for each microorganism.
- Published
- 2013
42. Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
- Author
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Ki Choon Choi, Jong Suk Lee, Srisesharam Srigopalram, Mayakrishnan Vijayakumar, Mariadhas Valan Arasu, Soundharrajan Ilavenil, Da Hye Kim, and Sang Gun Roh
- Subjects
0301 basic medicine ,Time Factors ,Gene Expression ,AMP-Activated Protein Kinases ,Mice ,chemistry.chemical_compound ,0302 clinical medicine ,Adipocytes ,lcsh:QH301-705.5 ,Cells, Cultured ,Medicine(all) ,AMPK-a ,Glucose Transporter Type 4 ,medicine.diagnostic_test ,Agricultural and Biological Sciences(all) ,Reverse Transcriptase Polymerase Chain Reaction ,Cell Differentiation ,Up-Regulation ,Biochemistry ,Adipogenesis ,Chlorella vulgaris ,Chemical constituents ,Adipocyte differentiation ,PPAR-gamma 2 ,AMPK-alpha ,030220 oncology & carcinogenesis ,PPAR-γ2 ,Lipogenesis ,Adiponectin ,Research Article ,medicine.drug ,Cell Survival ,Down-Regulation ,Biology ,03 medical and health sciences ,Western blot ,3T3-L1 Cells ,medicine ,Animals ,Oil Red O ,Plant Extracts ,Biochemistry, Genetics and Molecular Biology(all) ,Troglitazone ,AMPK ,Seaweed ,PPAR gamma ,AMPK-α ,Glucose ,030104 developmental biology ,lcsh:Biology (General) ,Diabetes Mellitus, Type 2 ,chemistry - Abstract
Background: From ancient times, marine algae have emerged as alternative medicine and foods, contains the rich source of natural products like proteins, vitamins, and secondary metabolites, especially Chlorella vulgaris (C. vulgaris) contains numerous anti-inflammatory, antioxidants and wound healing substances. Type 2 diabetes mellitus is closely associated with adipogenesis and their factors. Hence, we aimed to investigate the chemical constituents and adipogenic modulatory properties of C. vulgaris in 3T3-L1 pre-adipocytes. Results: We analysed chemical constituents in ethanolic extract of C. vulgaris (EECV) by LC-MS. Results revealed that the EECV contains few triterpenoids and saponin compounds. Further, the effect of EECV on lipid accumulation along with genes and proteins expressions which are associated with adipogenesis and lipogenesis were evaluated using oil red O staining, qPCR and western blot techniques. The data indicated that that EECV treatment increased differentiation and lipid accumulation in 3T3-L1 cells, which indicates positive regulation of adipogenic and lipogenic activity. These increases were associated with up-regulation of PPAR-gamma 2, C/EBP-alpha, adiponectin, FAS, and leptin mRNA and protein expressions. Also, EECV treatments increased the concentration of glycerol releases as compared with control cells. Troglitazone is a PPAR-gamma agonist that stimulates the PPAR-gamma 2, adiponectin, and GLUT-4 expressions. Similarly, EECV treatments significantly upregulated PPAR-gamma 2, adiponectin, GLUT-4 expressions and glucose utilization. Further, EECV treatment decreased AMPK-alpha expression as compared with control and metformin treated cells. Conclusion: The present research findings confirmed that the EECV effectively modulates the lipid accumulation and differentiation in 3T3-L1 cells through AMPK-alpha mediated signalling pathway.
- Published
- 2016
43. Protective effect ofLagenaria siceraria(Mol) against membrane-bound enzyme alterations in isoproterenol-induced cardiac damage in rats
- Author
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Mayakrishnan Vijayakumar, V. Selvi, and S. Krishnakumari
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medicine.medical_specialty ,ATPase ,Sodium ,Adrenergic beta-Antagonists ,chemistry.chemical_element ,Plant Science ,Biochemistry ,Analytical Chemistry ,Internal medicine ,Mole ,medicine ,Animals ,chemistry.chemical_classification ,biology ,Cell Membrane ,Organic Chemistry ,Membrane bound enzyme ,Isoproterenol ,Heart ,Lagenaria ,biology.organism_classification ,Treatment period ,Rats ,Cucurbitaceae ,Enzyme ,Endocrinology ,chemistry ,Heart tissues ,biology.protein - Abstract
This study was aimed at evaluating the preventive role of the ethanolic extract of Lagenaria siceraria (Mol) fruit on membrane-bound enzymes, such as sodium potassium-dependent adenosine triphosphatase (Na(+)/K(+) ATPase), calcium-dependent adenosine triphosphatase (Ca(2+) ATPase) and magnesium-dependent adenosine triphosphatase (Mg(2+) ATPase) on isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Male albino Wistar rats were pretreated with the ethanolic extract of L. siceraria (Mol) fruit (125, 250 and 500 mg kg(-1) body weight) for a period of 30 days. After the treatment period, ISO (85mg kg(-1) body weight) was subcutaneously injected into rats at 24-h intervals for 2 days. ISO-induced rats showed a significant (p 0.05) decrease in the activity of Na(+)/K(+) ATPase and an increase in the activities of Ca(2+) and Mg(2+) ATPases in the heart tissues. Pre-treatment with the ethanolic extract of L. siceraria (Mol) fruit for a period of 30 days exhibited a significant (p 0.05) effect in ISO-induced rats. Thus, our study shows that the ethanolic extract of L. siceraria (Mol) fruit has membrane-stabilising role in ISO-induced MI in rats.
- Published
- 2012
44. Cardioprotective effect ofLagenaria siceraria(Mol) ameliorates isoproterenol-induced cardiac toxicity in rats by stabilizing cardiac mitochondrial and lysosomal enzymes
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Mayakrishnan Vijayakumar, S. Krishnakumari, and V. Selvi
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chemistry.chemical_classification ,medicine.medical_specialty ,biology ,Health, Toxicology and Mutagenesis ,Succinate dehydrogenase ,Acid phosphatase ,Cathepsin D ,Lagenaria ,biology.organism_classification ,Pollution ,Malate dehydrogenase ,Enzyme ,Isocitrate dehydrogenase ,Endocrinology ,chemistry ,Oral administration ,Internal medicine ,biology.protein ,medicine ,Environmental Chemistry - Abstract
The present study was designed to evaluate the cardioprotective potential of Lagenaria siceraria (Mol) fruit on isoproterenol (ISO) induced myocardial infarction (MI) in rat model. Treatment with ISO 85 mg kg−1 body weight subcutaneously for 2 days significantly decreased the activities of cardiac mitochondrial enzymes isocitrate dehydrogenase, succinate dehydrogenase, and malate dehydrogenase. The activities of lysosomal enzymes including acid phosphatase, cathepsin D, and β-D-N-acetyl glucosaminidase were increased significantly in heart and serum. Pretreatment with L. siceraria (Mol) fruits for 30 days significantly elevated activities of cardiac mitochondrial enzymes and significantly lowered the activities of lysosomal enzymes in heart tissues of ISO-induced rats, which demonstrates that L. siceraria (Mol) fruits inhibits the actions of ISO on rat cardiac tissue. Oral administration of EELSF ethanolic extract of L. siceraria (Mol) (125, 250, or 500 mg kg−1 body weight) to normal rats did not show any...
- Published
- 2010
45. Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure
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Ki Choon Choi, S. Ravikumar, Hyung Soo Park, Mariadhas Valan Arasu, Soundharrajan Ilavenil, Mayakrishnan Vijayakumar, and Da Hye Kim
- Subjects
Article Subject ,Adaptation, Biological ,lcsh:Medicine ,Microbial Sensitivity Tests ,lcsh:Technology ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,Microbiology ,Bile Acids and Salts ,Probiotic ,law ,Lactobacillus ,RNA, Ribosomal, 16S ,Fusarium oxysporum ,Antibiosis ,Animals ,lcsh:Science ,General Environmental Science ,biology ,lcsh:T ,Probiotics ,lcsh:R ,Fungi ,food and beverages ,Penicillium roqueforti ,General Medicine ,Hydrogen-Ion Concentration ,biology.organism_classification ,Penicillium chrysogenum ,Anti-Bacterial Agents ,Manure ,Fermentation ,Metabolome ,lcsh:Q ,Lactobacillus plantarum ,Bacteria ,Research Article - Abstract
The aim of the study was to isolate and characterize the lactic acid bacteria (LAB) from animal manure. Among the thirty LAB strains, four strains, namely, KCC-25, KCC-26, KCC-27, and KCC-28, showed good cell growth and antifungal activity and were selected for further characterization. Biochemical and physiology properties of strains confirmed that the strains are related to theLactobacillussp.; further, the 16S rRNA sequencing confirmed 99.99% sequence similarity towardsLactobacillus plantarum. The strains exhibited susceptibility against commonly used antibiotics with negative hemolytic property. Strains KCC-25, KCC-26, KCC-27, and KCC-28 showed strong antifungal activity againstAspergillus fumigatus, Penicillium chrysogenum, Penicillium roqueforti, Botrytis elliptica, andFusarium oxysporum, respectively. Fermentation studies noted that the strains were able to produce significant amount of lactic, acetic, and succinic acids. Further, the production of extracellular proteolytic and glycolytic enzymes, survival under low pH, bile salts, and gastric juice together with positive bile salt hydrolase (Bsh) activity, cholesterol lowering, cell surface hydrophobicity, and aggregation properties were the strains advantages. Thus, KCC-25, KCC-26, KCC-27, and KCC-28 could have the survival ability in the harsh condition of the digestive system in the gastrointestinal tract. In conclusion, novelL. plantarumKCC-25, KCC-26, KCC-27, and KCC-28 could be considered as potential antimicrobial probiotic strains.
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- 2015
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46. Assessment of probiotic, antifungal and cholesterol lowering properties of Pediococcus pentosaceus KCC-23 isolated from Italian ryegrass
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Soundharrajan, Ilavenil, Mayakrishnan, Vijayakumar, Da Hye, Kim, Mariadhas, Valan Arasu, Hyung Su, Park, Sivanesan, Ravikumar, and Ki Choon, Choi
- Subjects
Gastric Juice ,Anticholesteremic Agents ,Probiotics ,Inulin ,Hydrogen-Ion Concentration ,Antioxidants ,Fungicides, Industrial ,Bile Acids and Salts ,Prebiotics ,Raffinose ,Italy ,Fermentation ,Lolium ,Pediococcus - Abstract
Lactic acid bacteria (LAB) are important for the processing of various food products. Although genetically modified organisms have contributed to improvements in various food products, there are some limitations. Thus, the discovery of wild strains from natural sources must be considered as the most suitable approach for identifying new LAB. Therefore, we planned to isolate and characterise the LAB from Italian ryegrass forage and evaluate their biological potential.A total of 28 strains were isolated and screened for their anti-fungal and probiotic properties. A single strain was selected due to its antifungal and probiotic efficiency. The strain was identified as Pediococcus pentosaceus KCC-23. The strain KCC-23 showed effective inhibition against Aspergillus fumigatus, Pencillium chrysogenum, Pencillium roqueforti, Botrytis elliptica and Fusarium oxysporum. Further, it survived low pH, and the presence of bile salts and gastric juice. It exhibited significant aggregation and hydrophobicity properties. The KCC-23 effectively assimilated cholesterol and had the ability to utilise pre-biotics such as raffinose and inulin. Finally, KCC-23 exhibited significant free radical scavenging activity.P. pentosaceus KCC-23 showed effective anti-fungal, probiotic and anti-oxidant properties and would be a promising isolate for exploitation in the formulation of food for ruminants and humans.
- Published
- 2014
47. The effect of nanoemulsified methionine and cysteine on the in vitro expression of casein in bovine mammary epithelial cells.
- Author
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Tae-Il Kim, Tae-Gyun Kim, Dong-Hyun Lim, Sang-Bum Kim, Seong-Min Park, Hyun-Joo Lim, Hyun-Jong Kim, Kwang-Seok Ki, Eung-Gi Kwon, Young-Jun Kim, and Mayakrishnan, Vijayakumar
- Subjects
METHIONINE ,CYSTEINE ,CASEINS ,ENDOTHELIAL cells ,DAIRY cattle - Abstract
Objective: Dairy cattle nutrient requirement systems acknowledge amino acid (AAs) requirements in aggregate as metabolizable protein (MP) and assume fixed efficiencies of MP used for milk protein. Regulation of mammary protein synthesis may be associated with AA input and milk protein output. The aim of this study was to evaluate the effect of nanoemulsified methionine and cysteine on the in-vitro expression of milk protein (casein) in bovine mammary epithelial cells (MAC-T cells). Methods: Methionine and cysteine were nonionized using Lipoid S 75 by high-speed homogenizer. The nanoemulsified AA particle size and polydispersity index were determined by dynamic light scattering correlation spectroscopy using a high-performance particle sizer instrument. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to determine the cytotoxicity effect of AAs with and without nanoionization at various concentrations (100 to 500 µg/mL) in mammary epithelial cells. MAC-T cells were subjected to 100% of free AA and nanoemulsified AA concentration in Dulbecco's modified Eagle medium/nutrient mixture F-12 (DMEM/F12) for the analysis of milk protein (casein) expression by the quantitative reverse transcription polymerase chain reaction method. Results: The AA-treated cells showed that cell viability tended to decrease (80%) in proportion to the concentration before nanogenesis, but cell viability increased as much as 90% after nanogenesis. The analysis of the expression of genetic markers related to milk protein indicated that; as2-casein increased 2-fold, γ-casein increased 5-fold, and the amount of unchanged ß-casein expression was nearly doubled in the nanoemulsified methionine-treated group when compared with the free-nanoemulsified methionine-supplemented group. On the contrary, the non-emulsified cysteine-administered group showed higher expression of genetic markers related to milk protein as2-casein, γ-casein, and ß-casein, but all the genetic markers related to milk protein decreased significantly after nanoemulsification. Conclusion: Detailed knowledge of factors, such nanogenesis of methionine, associated with increasing cysteine and decreasing production of genetic markers related to milk protein (casein) will help guide future recommendations to producers for maximizing milk yield with a high level of milk protein casein. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
48. Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
- Author
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Soundharrajan Ilavenil, Da Hye Kim, Mayakrishnan Vijayakumar, Srisesharam Srigopalram, Sang Gun Roh, Mariadhas Valan Arasu, Jong Suk Lee, and Ki Choon Choi
- Subjects
Chlorella vulgaris ,Chemical constituents ,Adipocyte differentiation ,PPAR-γ2 ,AMPK-a ,Biology (General) ,QH301-705.5 - Abstract
BACKGROUND: From ancient times, marine algae have emerged as alternative medicine and foods, contains the rich source of natural products like proteins, vitamins, and secondary metabolites, especially Chlorella vulgaris (C. vulgaris) contains numerous anti-inflammatory, antioxidants and wound healing substances. Type 2 diabetes mellitus is closely associated with adipogenesis and their factors. Hence, we aimed to investigate the chemical constituents and adipo-genic modulatory properties of C. vulgaris in 3T3-L1 pre-adipocytes. RESULTS: We analysed chemical constituents in ethanolic extract of C. vulgaris (EECV) by LC-MS. Results revealed that the EECV contains few triterpenoids and saponin compounds. Further, the effect of EECV on lipid accumulation along with genes and proteins expressions which are associated with adipogenesis and lipogenesis were evaluated using oil red O staining, qPCR and western blot techniques. The data indicated that that EECV treatment increased differentiation and lipid accumulation in 3T3-L1 cells, which indicates positive regulation of adipogenic and lipogenic activity. These increases were associated with up-regulation of PPAR-γ2, C/EBP-α, adiponectin, FAS, and leptin mRNA and protein expressions. Also, EECV treatments increased the concentration of glycerol releases as compared with control cells. Troglitazone is a PPAR-γ agonist that stimulates the PPAR-y2, adiponectin, and GLUT-4 expressions. Similarly, EECV treatments significantly upregulated PPAR-γ2, adiponectin, GLUT-4 expressions and glucose utilization. Further, EECV treatment decreased AMPK-α expression as compared with control and metformin treated cells. CONCLUSION: The present research findings confirmed that the EECV effectively modulates the lipid accumulation and differentiation in 3T3-L1 cells through AMPK-α mediated signalling pathway.
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- View/download PDF
49. Marine-derived Bioactive Peptides: Their Cardioprotective Activities and Potential Applications
- Author
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Kannappan Priya, A. Noorlidah, Ali Ahmed, Abdul Bakrudeen, Mayakrishnan Vijayakumar, and M. T. Rosna
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Nutraceutical ,Biochemistry ,Chemistry ,medicine ,Lipid metabolism ,Pharmacology ,medicine.disease_cause ,Anticoagulant activity ,Oxidative stress - Published
- 2013
50. Properties, Biological Advantages and Industrial Significance of Marine Peptides
- Author
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Noorlidah Abdullah, Rosna Mat Taha, Abdul Bakrudeen Ali Ahmed, Mayakrishnan Vijayakumar, and Ramjee Pallela
- Subjects
chemistry.chemical_compound ,Biochemistry ,Myoglobin ,chemistry ,Biology - Published
- 2013
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