1. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts
- Author
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Yan, Nie, Maxime, Chaillet, Christian, Becke, Matthias, Haffke, Martin, Pelosse, Daniel, Fitzgerald, Ian, Collinson, Christiane, Schaffitzel, and Imre, Berger
- Subjects
Automation, Laboratory ,Protein Conformation ,Genetic Vectors ,Gene Transfer Techniques ,Protein Engineering ,Recombinant Proteins ,High-Throughput Screening Assays ,Protein Subunits ,Structure-Activity Relationship ,Eukaryotic Cells ,Gene Expression Regulation ,Prokaryotic Cells ,Multiprotein Complexes ,Animals ,Humans ,Protein Multimerization ,Plasmids - Abstract
Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states. A multitude of applications in contemporary molecular and synthetic biology rely on efficient, robust and flexible methods to assemble multicomponent DNA circuits as a prerequisite to recapitulate such biological systems in vitro and in vivo. Numerous functionalities need to be combined to allow for the controlled realization of information encoded in a defined DNA circuit. Much of biological function in cells is catalyzed by multiprotein machines typically made up of many subunits. Provision of these multiprotein complexes in the test-tube is a vital prerequisite to study their structure and function, to understand biology and to develop intervention strategies to correct malfunction in disease states. ACEMBL is a technology concept that specifically addresses the requirements of multicomponent DNA assembly into multigene constructs, for gene delivery and the production of multiprotein complexes in high-throughput. ACEMBL is applicable to prokaryotic and eukaryotic expression hosts, to accelerate basic and applied research and development. The ACEMBL concept, reagents, protocols and its potential are reviewed in this contribution.
- Published
- 2016