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In a 1-day old filly with a loud heart murmur, transthoracic echocardiography revealed right ventricular hypertrophy associated with severe pulmonary valvular stenosis and a transvalvular pressure gradient (between right ventricle and pulmonary artery) of 125 mmHg. Computed tomographic angiography confirmed the finding, with no evidence of other relevant concurrent abnormalities. Balloon valvuloplasty was performed using a single balloon technique. The foal recovered well from anaesthesia. Following the procedure, the right ventricle-pulmonary artery transvalvular gradient decreased to 38 mmHg. At follow up examinations after 1 month, 1 year and 2 years, the filly showed normal exercise capacity and echocardiography confirmed the persistent substantial improvement in the transvalvular outflow gradient.

INTRODUCTION This case report describes a 12-year-old female spayed mixed-breed dog referred for treatment of a large, inoperable hepatocellular carcinoma. A computed tomography (CT) scan confirmed the previous ultrasonographic and laparoscopic findings of a large, lobulated, poorly defined mass on the left and central aspect of the liver. Multiple biopsies confirmed the diagnosis of hepatocellular carcinoma. Due to the large extent of the tumor, the vascular association to the Vena cava caudalis and the associated high risk of intraoperative bleeding, a resection of the mass was refrained from and a radiotherapeutic treatment was chosen. The dog underwent radiation therapy (RT) with a 6MV linear accelerator with 5×6 Gy, total dose 30 Gy. In the follow up examinations three months and one year after therapy, the dog presented in normal condition and had normal Alanine-amino-transferase (ALT) and alkaline phosphatase (AP). The tumor size measured in the CT-examinations decreased by 61% and 90%, respectively. Two years after radiation therapy the dog has a normal general condition and liver enzymes are within the normal limits.

Cortical atrophy has been identified using magnetic resonance imaging (MRI) in humans and dogs with Lafora disease (LD). In humans, proton magnetic resonance spectroscopy (1HMRS) of the brain indicates decreased N‐acetyl‐aspartate (NAA) relative to other brain metabolites. Brain 1HMRS findings in dogs with LD are lacking. A 6‐year‐old female Beagle was presented with a history of a single generalized tonic‐clonic seizure and episodic reflex myoclonus. Clinical, hematological, and neurological examination findings and 3‐Tesla MRI of the brain were unremarkable. Brain 1HMRS with voxel positioning in the thalamus was performed in the affected Beagle. It identified decreased amounts of NAA, glutamate‐glutamine complex, and increased total choline and phosphoethanolamine relative to water and total creatine compared with the reference range in healthy control Beagles. A subsequent genetic test confirmed LD. Abnormalities in 1HMRS despite lack of changes with conventional MRI were identified in a dog with LD.

The prevalence of osteochondral changes in the metatarsal growth plates of fattening bulls was investigated. Plantarodorsal radiographic views of the metatarsal region of 204 hind legs from 102 slaughtered fattening bulls were taken. Radiographic lesions in metatarsal growth plates were confirmed in selected cases using computed tomography (n=14) and histology (n=6). Radiographic criteria for growth plate lesions were axis deviations between the metatarsal bones and the digit, radiolucencies in the growth plate, increased bone density in the metaphysis, irregularities of the growth plate and marginal osteophytes («lipping»). The individual lesions were categorised as present/absent or 1 = slight, 2 = distinct, and 3 = severe. A weighted sum of these radiographic changes was generated for each growth plate and for each animal, and used for statistical analysis of the associations between husbandry factors and the occurrence of lesions. Almost all of the metatarsal bones had signs of osteochondral changes on the radiographs of the growth plates. The most common lesions were lipping (99,5 %), increased bone density in the metaphysis (66,6 %) and radiolucencies in the growth plates (64,1 %). Computed tomography and histology revealed hyperplasia of the margins of the epiphysis and metaphysis, focal increase in bone deposits accompanied by incomplete ossification and irregular and widened cartilage columns of the growth plate. There were statistically significant associations between the weighted radiographic changes and slaughter weight, average daily weight gain, husbandry practices and production label. The prevalence of radiographic lesions indicative for osteochondrosis of the metatarsal growth plates of fattening bulls slaughtered at a local abattoir was high. The extent to which the distinct and severe lesions are associated with reduced production and obvious clinical lameness requires further study.Die Prävalenz von osteochondrotischen Veränderungen in den Epiphysenfugen von Maststieren sollte anhand der Ossa metatarsalia postmortal dokumentiert werden. Dazu wurden 204 Hinterfüsse von 102 Maststieren gesammelt und röntgenologisch im plantarodorsalen Strahlengang untersucht. Bei ausgewählten Ossa metatarsalia wurden die Veränderungen computertomographisch (n=14) und histologisch (n=6) detaillierter dargestellt. Radiologische Kriterien für die Befundung waren Weichteilschwellung, Achsenabweichungen zwischen Röhrbein- und Zehenachse, Aufhellungen in der Wachstumszone, vermehrte Dichte des an die Epiphysenfuge angrenzenden Knochens, Regelmässigkeit und Weite der Epiphysenfugenlinien sowie knöcherne Ausziehungen («Lippenbildung») am Rande der Epiphysenfuge. Die Veränderungen wurden je nach Kriterium in vorhanden/nicht vorhanden oder 1 = schwach, 2 = deutlich und 3 = stark gewichtet. Aus den Befunden wurden gewichtete Röntgensummen der Veränderungen pro Metatarsus und pro Tier für die statistische Auswertung erstellt. Mit Hilfe dieser Röntgensumme wurde statistisch untersucht, ob ein Zusammenhang der Röntgenveränderungen mit verschiedenen Faktoren der Haltung bestand. Von den 204 Ossa metatarsalia wiesen nahezu alle röntgenologische Veränderungen im Sinne einer Osteochondrose an den Epiphysenfugen auf. Die häufigsten Befunde waren eine «Lippenbildung» (99,5 %), erhöhte Dichte des Knochengewebes nahe den Fugenlinien (66,6 %) und Aufhellungen in den Fugen (64,1 %). Die entsprechenden computertomographisch und histologisch nachweisbaren Veränderungen waren eine Hyperplasie des Randbereichs der Epiphyse und der Metaphyse, vermehrte Knochendichte bei unvollständiger Verknöcherung und eine Unregelmässigkeit sowie Verbreiterung der Säulenknorpelzone. Ein signifikanter Zusammenhang der gewichteten röntgenologischen Veränderungen mit dem Schlachtgewicht, mit der durchschnittlichen täglichen Gewichtszunahme, mit dem Produzenten sowie mit dem Produktionslabel war statistisch nachzuweisen. Zusammenfassend konnte röntgenologisch eine hohe Prävalenz von osteochondrotischen Veränderungen der Epiphysenfugen der Ossa metatarsalia von Maststieren aus dem Einzugsbereich des lokalen Schlachthofs festgestellt werden. Der Zusammenhang der deutlichen und insbesondere der starken Schädigungen mit einer reduzierten Mastleistung und einer klinischen Lahmheit bedarf weiterer Untersuchungen.La prévalence des modifications ostéochondrales des plaques de croissance métatarsiennes des taureaux à l’engraissement a été étudiée. Des radiographies planto-dorsales de la région métatarsienne de 204 membres postérieurs de 102 taureaux d’engraissement abattus ont été réalisées. Les lésions radiographiques des plaques de croissance métatarsiennes ont été confirmées dans des cas sélectionnés par tomodensitométrie (n=14) et histologie (n=6). Les critères radiographiques des lésions du cartilage de conjugaison étaient les déviations de l’axe entre les os métatarsiens et les phalanges, les radiotransparences du cartilage de conjugaison, l’augmentation de la densité osseuse de la métaphyse, les irrégularités du cartilage de conjugaison et les ostéophytes marginaux (« lipping »). Les lésions individuelles ont été classées comme présentes/absentes ou 1 = légères, 2 = distinctes et 3 = sévères. Une somme pondérée de ces modifications radiographiques a été générée pour chaque plaque de croissance et pour chaque animal et utilisée pour l’analyse statistique des associations entre les facteurs d’élevage et l’apparition de lésions. Presque tous les os métatarsiens présentaient des signes de modifications ostéochondrales sur les radiographies des plaques de croissance. Les lésions les plus fréquentes étaient le « lipping » (99,5 %), l’augmentation de la densité osseuse au niveau de la métaphyse (66,6 %) et les radiotransparences des plaques de croissance (64,1 %). La tomodensitométrie et l’histologie ont révélé une hyperplasie des marges de l’épiphyse et de la métaphyse, une augmentation focale des dépôts osseux accompagnée d’une ossification incomplète et des colonnes cartilagineuses irrégulières et élargies sur le cartilage de conjugaison. Il y avait des associations statistiquement significatives entre les changements radiographiques pondérés et le poids d’abattage, le gain de poids quotidien moyen, les pratiques d’élevage et les labels de production. La prévalence des lésions radiographiques révélatrices d’une ostéochondrose des plaques de croissance métatarsiennes des taureaux d’engraissement abattus dans un abattoir local était élevée. La mesure dans laquelle ces lésions distinctes et sévères sont associées à une production réduite et à une boiterie clinique évidente nécessite une étude plus approfondie.La prevalenza dei cambiamenti osteocondrali nelle articolazioni epifisarie dei tori da ingrasso doveva essere documentata, per questo studio, post-mortem utilizzando le ossa metatarsali. Sono state quindi raccolti ed esaminati radiograficamente 204 piedi posteriori di 102 tori da ingrasso nel percorso fascio plantarodorsale. Le lesioni delle ossa metatarsali sono state confermate in casi selezionati utilizzando la tomografia computerizzata (n=14) e l’istologia (n=6). I criteri radiografici dei risultati delle lesioni erano il gonfiore dei tessuti molli, la deviazioni dell’asse tra il metatarso e il piede, schiarimenti nella zona di crescita, aumento della densità dell’osso adiacente all’articolazione epifisaria, regolarità e larghezza delle linee dell’articolazione epifisaria così come estrazioni ossee («lipping») al bordo dell’articolazione epifisaria. Le singole lesioni sono state classificate come presenti/assenti o 1 = lievi, 2 = evidenti e 3 = gravi. Una somma ponderata di questi cambiamenti radiografici è stata generata per ogni metatarso e per ogni animale, e utilizzata per l’analisi statistica delle associazioni tra i fattori di allevamento e la comparsa delle lesioni. Tra le 204 ossa metatarsali raccolte, quasi tutte evidenziavano cambiamenti radiografici nel senso di osteocondrosi alle articolazioni epifisarie. Le lesioni più comuni erano il lipping (99,5 %), l’aumento della densità ossea vicino alle linee articolari (66,6 %) e gli schiarimenti nella zona di crescita (64,1 %). La tomografia computerizzata e l’istologia hanno rivelato un’iperplasia dei margini dell’epifisi e della metafisi, e un aumento focale dei depositi ossei accompagnato da un’ossificazione incompleta e colonne cartilaginee irregolari e allargate della placca di crescita. Sono state rilevate associazioni statisticamente significative tra i cambiamenti radiografici ponderati e il peso di macellazione, l’aumento di peso medio giornaliero, le pratiche di allevamento e l’etichetta di produzione. La prevalenza di lesioni radiografiche indicative di osteocondrosi delle placche di accrescimento metatarsali dei tori da ingrasso macellati in un mattatoio locale era alta. La misura in cui queste lesioni distinte e gravi sono associate a una produzione ridotta e a una zoppia clinica evidente richiede ulteriori studi.

OBJECTIVE:To compare the quality of visualization of canine carpal ligaments by using computed tomography (CT), MRI, CT arthrography (CTA), and magnetic resonance arthrography (MRA). STUDY DESIGN: Prospective descriptive study. STUDY POPULATION: Cadavers from dogs weighing more than 20 kg. METHODS: A 16-slice CT scanner and a 3 Tesla MRI were used for the investigation. A dilute contrast medium was injected into the middle carpal and radiocarpal joints under fluoroscopic control, and CTA and MRA images were acquired. To evaluate the difference between imaging modalities, 3 observers graded carpal ligaments of clinical interest using a scale from 0 to 4 for their quality of visualization. Data were analyzed by using a random-effect ordinal logistic regression with Bonferroni adjustment. The interobserver agreement was calculated by using the weighted Cohen's κ. RESULTS: Normal carpal joints (n = 9) were investigated. Magnetic resonance arthrography improved visualization of the majority of carpal ligaments compared with MRI (P

Ten healthy Beagle dogs were used to compare selected echocardiographic parameters of right ventricular (RV) systolic function with high field cardiac magnetic resonance imaging (MRI). All dogs underwent complete transthoracic echocardiography before and during anesthesia, as well as cardiac MRI with determination of morphology-based ejection fraction, and phase contrast angiography-derived stroke volume. A correlation analysis was carried out between echocardiographic and MRI parameters. The values of fractional area change, tricuspid annular plane systolic excursion, tissue Doppler imaging derived peak velocity of the isovolumic contraction wave and systolic wave of the lateral tricuspid valve annulus showed lower values under general anesthesia. Of all echocardiographic parameters of RV systolic function, only fractional area change (r = 0.671, P = 0.034) and tissue Doppler imaging derived peak velocity of the systolic wave of the lateral tricuspid valve annulus (r = 0.768; P = 0.01), showed a significant correlation with MRI derived stroke volume. None of the echocardiographic parameters correlated with MRI derived ejection fraction. When evaluating the RV echocardiographically, fractional area change and peak velocity of the systolic wave of the lateral tricuspid valve annulus appear to be the most reliable predictors of RV systolic function when compared to MRI under anesthesia.

Background Cerebrospinal fluid (CSF) flow in disease has been investigated with two-dimensional (2D) phase-contrast magnetic resonance imaging (PC-MRI) in humans. Despite similar diseases occurring in dogs, PC-MRI is not routinely performed and CSF flow and its association with diseases is poorly understood. Objectives To adapt 2D and four-dimensional (4D) PC-MRI to dogs and to apply them in a group of neurologically healthy dogs. Animals Six adult Beagle dogs of a research colony. Methods Prospective, experimental study. Sequences were first optimized on a phantom mimicking small CSF spaces and low velocity flow. Then, 4D PC-MRI and 2D PC-MRI at the level of the mesencephalic aqueduct, foramen magnum (FM), and cervical spine were performed. Results CSF displayed a bidirectional flow pattern on 2D PC-MRI at each location. Mean peak velocity (and range) in cm/s was 0.92 (0.51-2.08) within the mesencephalic aqueduct, 1.84 (0.89-2.73) and 1.17 (0.75-1.8) in the ventral and dorsal subarachnoid space (SAS) at the FM, and 2.03 (range 1.1-3.0) and 1.27 (range 0.96-1.82) within the ventral and dorsal SAS of the cervical spine. With 4D PC-MRI, flow velocities of >3 cm/s were visualized in the phantom, but no flow data were obtained in dogs. Conclusion Peak flow velocities were measured with 2D PC-MRI at all 3 locations and slower velocities were recorded in healthy Beagle dogs compared to humans. These values serve as baseline for future applications. The current technical settings did not allow measurement of CSF flow in Beagle dogs by 4D PC-MRI.

Resting-state functional Magnetic Resonance Imaging (rs-fMRI) has become an established technique in humans and reliably determines several resting state networks (RSNs) simultaneously. Limited data exist about RSN in dogs. The aim of this study was to investigate the RSNs in 10 healthy beagle dogs using a 3 tesla MRI scanner and subsequently perform group-level independent component analysis (ICA) to identify functionally connected brain networks. Rs-fMRI sequences were performed under steady state sevoflurane inhalation anaesthesia. Anaesthetic depth was titrated to the minimum level needed for immobilisation and mechanical ventilation of the patient. This required a sevoflurane MAC between 0.8 to 1.2. Group-level ICA dimensionality of 20 components revealed distributed sensory, motor and higher-order networks in the dogs’ brain. We identified in total 7 RSNs (default mode, primary and higher order visual, auditory, two putative motor-somatosensory and one putative somatosensory), which are common to other mammals including humans. Identified RSN are remarkably similar to those identified in awake dogs. This study proves the feasibility of rs-fMRI in anesthetized dogs and describes several RSNs, which may set the basis for investigating pathophysiological characteristics of various canine brain diseases.

Public interest in animal tuberculosis is mainly focused on prevention and eradication of bovine tuberculosis in cattle and wildlife. In cattle, immunodiagnostic tests such as the tuberculin skin test or the interferon gamma (IFN-γ) assay have been established and are commercially available. Feline tuberculosis is rather unknown, and the available diagnostic tools are limited. However, infections with Mycobacterium tuberculosis complex members need to be considered an aetiological differential diagnosis in cats with granulomatous lymphadenopathy or skin nodules and, due to the zoonotic potential, a time-efficient and accurate diagnostic approach is required. The present study describes 11 independent cases of Mycobacterium microti infection in domestic cats in Switzerland. For three cases, clinical presentation, diagnostic imaging, bacteriological results, immunodiagnostic testing, and pathological features are reported. An adapted feline IFN-γ release assay was successfully applied in two cases and appears to be a promising tool for the ante mortem diagnosis of tuberculosis in cats. Direct contact with M. microti reservoir hosts was suspected to be the origin of infection in all three cases. However, there was no evidence of M. microti infection in 346 trapped wild mice from a presumptive endemic region. Therefore, the source and modalities of infection in cats in Switzerland remain to be further elucidated.

With the growing interest in cardiac magnetic resonance imaging (cMRI), veterinary radiologists will increasingly be asked to use this modality to answer complex cardiological questions. Plane alignment is crucial for reproducible assessment of the heart. Anesthesia time is a limiting factor in cMRI. Aims of this prospective experimental study were to introduce a flow chart for standardized cMRI-examination in dogs, to test it for reproducibility using a cardiac CT simulation and to estimate time requirements needed to complete the examination accurately. Six operators (3 radiologists, 1 cardiologist, 1 imaging-resident, 1 technician) simulated a cMRI examination on CT-scans of 6 healthy Beagle dogs twice within two to four weeks. Assessment included qualitative and quantitative scoring of plane quality and time requirements. The quality of planes was high for the left and moderate for the right side of the heart. The intraclass correlation coefficient (ICC) of linear measurements of structures on the left was good to excellent (ICC-range: 0.789-0.948) but dropped to moderate to poor levels for the right side (ICC-range: 0.429-0.738). The median time required to complete a full examination was 30 (range: 13-103) min in the first and 24 (range: 15-62) min in the second evaluation. It differed significantly between operators and was consistently shorter for the left than for the right side. In conclusion, a new standardized scheme for cMRI can be quickly adopted by radiologists with some expertise in cross sectional imaging. Qualitative and quantitative results were highly reproducible for the left but less for the right side.

A pet rabbit was found dead outside its enclosure; the head was missing. The investigation of cruelty to animals was commissioned, as decapitation might result from human mutilation, for example, due to occult rituals or neighbourhood disputes. A first external examination showed circular disruption rostral of the pectoral girdle with lack of the head and palpable bone fragments of both forelimbs. CT scan revealed extensive, irregular trauma to soft tissues and skeleton. The decapitation left a cut-like but irregular, partially wave-like wound margin with multiple small triangular, divergent defects more closely examined by postmortem examination. Swabs from the margin of the wound and tissue samples were collected for forensic genetic analysis and histology. Considering these interdisciplinary investigation results combined with experts’ knowledge, a predator attack was highly likely. Further evidence on geographical distribution and physiological behaviour of putative predator species led to consensus on the identity of the most likely perpetrator: a red fox.

Blood flow changes in cranial abdominal vessels are important contributing factors for canine hepatic disease. This prospective, experimental, pilot study aimed to evaluate cardiac-gated, phase contrast magnetic resonance angiography (PCMRA) as a method for characterizing blood flow in canine major cranial abdominal vessels. Eleven, healthy, adult beagle dogs were sampled. Cardiac-gated, phase contrast magnetic resonance angiography of the cranial abdomen was performed in each dog and blood flow was independently measured in each of the major cranial abdominal vessels by three observers, with two observers recording blood flow values once and one observer recording blood flow values three times. Each dog then underwent ultrasonographic examination of the liver with fine needle aspirations and biopsies submitted to cytologic and histologic examination. The mean absolute stroke volume and velocity were respectively 9.6 ± 1.9 ml and -11.1 ± 1.1 cm/s for the cranial abdominal aorta, 2.1 ± 0.6 ml and -6.6 ± 1.9 cm/s for the celiac artery, and 2.3 ± 1.0 ml and -7.9 ± 3.1 cm/s for the cranial mesenteric artery. The mean absolute stroke volume and velocity were respectively 6.7 ± 1.3 ml and 3.9 ± 0.9 cm/s for the caudal vena cava and 2.6 ± 0.9 ml and 3.2 ± 1.2 cm/s for the portal vein. Intraobserver reliability was excellent (intraclass correlation coefficient > 0.9). Interobserver reproducibility was also excellent (intraclass correlation coefficient 0.89-0.99). Results of liver ultrasonography, cytology, and histopathology were unremarkable. Findings indicated that cardiac-gated, phase contrast magnetic resonance angiography is a feasible technique for quantifying blood blow in canine major cranial abdominal vessels. Blood flow values from this sample of healthy beagles can be used as background for future studies on canine hepatic disease.

A 3-year-old French bulldog was presented to the ophthalmology service of the Vetsuisse Faculty, University of Zurich with a 3-day history of conjunctival swelling of the left eye (OS). Ophthalmologic examination revealed a moderate conjunctival hyperemia and chemosis. A migrating foreign body having entered the conjunctival fornix behind the nictitating membrane was suspected. Within the first 24 hours of medical management, OS developed a panuveitis and a scleral perforation was highly suspected. Ocular and orbital ultrasound as well as conventional magnetic resonance imaging (MRI) examinations failed to confirm the presence of a perforating foreign body. A High-Resolution MRI (HR-MRI) using a microscopy coil was then performed with findings consistent with a perforating and migrating foreign body. A grass awn of 12 mm length was surgically retrieved "ab externo" from its' point of entry into the sclera. To the best of our knowledge, HR-MRI has not yet been used to examine canine eyes. This case report supports the idea that orbital imaging can be greatly enhanced with the introduction of HR-MRI using microscopy coils with clinically relevant implications.

Delayed ischemic neurological deficit (DIND) is a major driver of adverse outcomes in patients with aneurysmal subarachnoid hemorrhage (aSAH), defining an unmet need for therapeutic development. Cell-free hemoglobin that is released from erythrocytes into the cerebrospinal fluid (CSF) is suggested to cause vasoconstriction and neuronal toxicity, and correlates with the occurrence of DIND. Cell-free hemoglobin in the CSF of patients with aSAH disrupted dilatory NO signaling ex vivo in cerebral arteries, which shifted vascular tone balance from dilation to constriction. We found that selective removal of hemoglobin from patient CSF with a haptoglobin-affinity column or its sequestration in a soluble hemoglobin-haptoglobin complex was sufficient to restore physiological vascular responses. In a sheep model, administration of haptoglobin into the CSF inhibited hemoglobin-induced cerebral vasospasm and preserved vascular NO signaling. We identified 2 pathways of hemoglobin delocalization from CSF into the brain parenchyma and into the NO-sensitive compartment of small cerebral arteries. Both pathways were critical for hemoglobin toxicity and were interrupted by the large hemoglobin-haptoglobin complex that inhibited spatial requirements for hemoglobin reactions with NO in tissues. Collectively, our data show that compartmentalization of hemoglobin by haptoglobin provides a novel framework for innovation aimed at reducing hemoglobin-driven neurological damage after subarachnoid bleeding.

Acromegaly due to a pituitary tumor has so far only been described in 3 dogs. The present case report describes a 7-year-old male-castrated Labrador Retriever which was referred because of difficult-to-control diabetes. Physical examination revealed markedly enlarged head, tongue and paws, widened interdental spaces and thickening of the skin in the head and neck area. IGF-1 and GH were increased and the latter continued to be abnormal after somatostatin application. Computed tomography demonstrated a space-occupying lesion in the pituitary gland and the diagnosis of acromegaly due to a GH-producing tumor of the pituitary was made. The dog underwent radiation therapy with a 6MV linear accelerator (3×8Gy) and improved substantially. Two and a half years after radiation therapy the dog developed lethargy and anorexia and was euthanized. Necropsy was not permitted. This case report represents the description of a dog suffering from pituitary-dependent acromegaly which was successfully treated and had a long-term survival.Eine Akromegalie aufgrund eines Hypophysentumors wurde bisher erst bei drei Hunden beschrieben. Der Fallbericht beschreibt einen 7-jährigen männlich-kas­trierten Labrador Retriever, der wegen eines schlecht einstellbaren Diabetes mellitus überwiesen wurde. Der Hund zeigte einen vergrösserten Kopfumfang, vergrösserte Zunge und Pfoten, erweiterte Interdentalspalten, sowie verdickte Haut im Kopf- und Halsbereich. Die IGF-1- und STH-Konzentrationen waren erhöht und die STH-Konzentration blieb nach Applikation von Soma­tostatin abnormal hoch. Mittels Computertomographie wurde eine Masse im Bereich der Hypophyse nachgewiesen und es wurde die Diagnose Akromegalie aufgrund eines STH-produzierenden Tumors in der Hypophyse gestellt. Der Hund erhielt eine Strahlentherapie mittels 6MV Linearbeschleuniger (3×8Gy) und sein Zustand verbesserte sich substantiell. Nach 2½ Jahren entwickelte er Anorexie und Apathie und wurde ­euthanasiert. Eine Obduktion konnte nicht durchgeführt werden. Es handelt sich um den Bericht eines Hundes mit einem STH-produzierenden Hypophysentumor, der erfolgreich behandelt wurde und eine lange Überlebenszeit hatte.L’acromégalie due à une tumeur hypophysaire n’a jusqu’à présent été décrite que chez 3 chiens. Le présent rapport de cas décrit un Labrador Retriever de 7 ans mâle castré, qui a été référé en raison d’un diabète difficile à contrôler. L’examen physique a révélé une tête, une langue et des pattes de taille nettement augmentée, des espaces interdentaires élargis et un épaississement de la peau dans la région de la tête et du cou. L’IGF-1 et la GH étaient augmentées et la seconde restait anormale après l’application de somatostatine. La tomodensitométrie a mis en évidence une masse dans la région de l’hypophyse et le diagnostic d’acromégalie due à une tumeur de l’hypophyse productrice de GH a été posé. Le chien a subi une radiothérapie avec un accélérateur linéaire de 6MV (3×8Gy) et son état s’est considérablement amélioré. Deux ans et demi après la radiothérapie, le chien développa une léthargie et une anorexie et fut euthanasié. L’autopsie n’a pas été autorisée. Ce rapport de cas représente la description d’un chien souffrant d’acromégalie dépendant de l’hypophyse, traité avec succès et ayant une survie à long terme.L’acromegalia a causa di un tumore ipofisario è stata finora descritta solo in 3 cani. Il presente studio descrive un Labrador Retriever maschio castrato di 7 anni che è stato ammesso a causa di un diabete difficile da controllare. L’esame fisico ha rivelato un marcato ingrandimento della testa, della lingua e delle zampe, spazi interdentali più ampi e un ispessimento della pelle nella zona della testa e del collo. I valori di IGF-1 e GH erano aumentati e il valore di GH è rimasto anormale anche dopo la somministrazione di somatostatina. La tomografia computerizzata ha mostrato una massa nella zona dell’ipofisi ed è stata diagnosticata una acromegalia causata da un tumore che produce GH nell’ipofisi. Il cane è stato sottoposto a radioterapia con un acceleratore lineare 6MV (3×8Gy) e il suo stato è migliorato sostanzialmente. Due anni e mezzo dopo la radioterapia il cane ha sviluppato letargia e anoressia ed è stato sottoposto a eutanasia. La necroscopia non è stata consentita. Questo caso mostra un cane affetto da acromegalia dipendente dall’ipofisi, che è stato trattato con successo e ha avuto una sopravvivenza a lungo termine.

OBJECTIVE To compare values of CT-derived glomerular filtration rate (GFR) determined by 3 contrast-medium injection protocols and 4 measurement techniques in healthy Beagles. ANIMALS 9 healthy Beagles (mean ± SD weight, 13.2 ± 1.6 kg). PROCEDURES Each dog underwent 3 iohexol-injection protocols (700 mg of iodine/kg administered at a constant rate over 20 seconds, 700 mg of iodine/kg administered following an exponentially decelerated injection over 20 seconds, and 350 mg of iodine/kg at a constant rate over 10 seconds) during dynamic, whole renal-volume CT in randomized order with an interval of ≥ 7 days between experiments. Values of GFR determined from Patlak plots derived by use of 4 measurement techniques (standard transverse section, optimized transverse section, dorsal reconstruction, and volume calculation techniques) were compared. RESULTS The measurement technique influenced the mean ± SD GFR results (standard transverse section technique, 2.49 ± 0.54 mL/kg/min; optimized transverse section technique, 2.72 ± 0.52 mL/kg/min; dorsal reconstruction technique, 3.00 ± 0.60 mL/kg/min, and volume calculation technique, 2.48 ± 0.51 mL/kg/min). The lower iodine dose resulted in a significantly higher GFR value (3.00 ± 0.65 mL/kg/min), compared with that achieved with either higher dose administration (constant rate injection, 2.54 ± 0.45 mL/kg/min and exponentially decelerated injection, 2.47 ± 0.48 mL/kg/min). CONCLUSIONS AND CLINICAL RELEVANCE In healthy Beagles, the CT-derived GFR measurements obtained after injection of a full dose of contrast medium were reduced, compared with measurements obtained after injection of a half dose. This finding is important with regard to potential nephrotoxicosis in dogs with impaired renal function and for GFR measurement with CT-contrast medium protocols.

This report describes a two-month-old Ouessant ram lamb with hind limb ataxia, knuckling and falling attributable to an abscess in the thoracic vertebral region. A swelling palpated adjacent to the spinous processes of the 9th to 12th thoracic vertebrae was identified via ultrasonography as an abscess located on the transverse processes of these vertebrae. Magnetic resonance imaging of the lamb postmortem revealed that the abscess had invaded the vertebral canal through a channel at the level of the 9th thoracic vertebra causing extradural spinal cord compression. Postmortem examination confirmed these findings, and histological examination showed acute spinal cord degeneration.Es wird ein 2 Monate altes, männliches Ouessant-Schaf mit Abszess im Bereich der thorakalen Wirbelsäule beschrieben. Die wichtigsten klinische Befunde waren Nachhandataxie, Überköten hinten beidseits, Schwanken der Nachhand und Umfallen. Bei der Adspektion und Palpation wurde neben den Dornfortsätzen der Brustwirbel 9 bis 12 eine Umfangsvermehrung festgestellt. Diese stellte sich bei der Ultraschalluntersuchung als kavitäre Läsion dar (Verdacht auf Abszessbildung), welche seitlich auf den Querfortsätzen der Brustwirbel lag. Das Schaf wurde euthanasiert. Bei der postmortem durchgeführten MRT-Untersuchung (Magnetresonanztomographie) konnten Ausmass und Lage des Abszesses genau charakterisiert werden. Auf Höhe von T9 war ein Kanal zu sehen, der vom Abszess ausging und sich in den Wirbelkanal erstreckte und das Rückenmark komprimierte. Die Sektion bestätigte die MRI-Befunde. Histologisch war an der Kompressionsstelle eine akute Degeneration des Rückenmarks zu sehen, welche für die klinischen Symptome verantwortlich war.

OBJECTIVE To investigate metabolite concentrations of the brains of dogs with intracranial neoplasia or noninfectious meningoencephalitis by use of short echo time, single voxel proton magnetic resonance spectroscopy (1H MRS) at 3.0 T. ANIMALS 29 dogs with intracranial lesions (14 with neoplasia [3 oligodendromas, 3 glioblastomas multiformes, 3 astrocytomas, 2 lymphomas, and 3 meningiomas] and 15 is with noninfectious meningoencephalitis) and 10 healthy control dogs. PROCEDURES Short echo time, single voxel 1H-MRS at 3.0 T was performed on neoplastic and noninfectious inflammatory intracranial lesions identified with conventional MRI. Metabolites of interest included N-acetyl aspartate (NAA), total choline, creatine, myoinositol, the glutamine-glutamate complex (Glx), glutathione, taurine, lactate, and lipids. Data were analyzed with postprocessing fitting algorithm software. Metabolite concentrations relative to brain water content were calculated and compared with results for the healthy control dogs, which had been previously evaluated with the same 1H MRS technique. RESULTS NAA, creatine, and Glx concentrations were reduced in the brains of dogs with neoplasia and noninfectious meningoencephalitis, whereas choline concentration was increased. Concentrations of these metabolites differed significantly between dogs with neoplasia and dogs with noninfectious meningoencephalitis. Concentrations of NAA, creatine, and Glx were significantly lower in dogs with neoplasia, whereas the concentration of choline was significantly higher in dogs with neoplasia. Lipids were predominantly found in dogs with high-grade intra-axial neoplasia, meningioma, and necrotizing meningoencephalitis. A high concentration of taurine was found in 10 of 15 dogs with noninfectious meningoencephalitis. CONCLUSIONS AND CLINICAL RELEVANCE 1H MRS provided additional metabolic information about intracranial neoplasia and noninfectious meningoencephalitis in dogs.

Corpus callosum abnormalities (CCA) rarely occur in dogs and are related to hypo/adypsic hypernatremia and seizures. Hypoplasia and dysplasia of the corpus callosum (CC) with concomitant lobar holoprosencephaly is the most common variant. It is currently uncertain using conventional MRI if canine CCA reflects the failure of commissural fibers to develop or the failure of the commissural fibers to cross hemispheres. Diffusion tensor imaging was performed in a 4-year-old Staffordshire mix breed dog with CCA and an age-matched healthy Beagle. In comparison to the control dog, CC tractography of the affected dog depicted only axonal tracts corresponding to the temporal CC fibers. The cingulum bundles appeared supernumerary with unorganized architecture, extending into the ipsilateral cerebral cortex, and therefore strongly suggested homology to Probst bundles reported in humans with CCA. The presence of Probst bundles in canine CCA could represent compensatory neuroplasticity-mediated networking and may contribute the fair prognosis reported in affected dogs.

A 2-year-old Airedale terrier was presented with exercise intolerance since birth and newly developed chylous pleural effusion. Imaging procedures including echocardiography, cardiac magnetic resonance imaging, computed tomography, and selective angiography revealed an aberrant connection of the azygos vein and the left atrium, a membrane in the right atrium consistent with cor triatriatum dexter, and a patent foramen ovale with right-to-left shunt. Balloon dilation of the membrane in the right atrium seemed to result in transient improvement of exercise tolerance compared with the previous 2 years. When chylothorax relapsed after three months, the dog was euthanized. Necropsy confirmed the azygos vein to left atrial connection, the patent foramen ovale, and the cor triatriatum dexter.

Objective: To assess the feasibility of myocardial perfusion analysis in healthy dogs using dynamic contrast-enhanced cardiac magnetic resonance (DCE-MR) imaging at rest and during simulated stress with two doses of adenosine. Animals: Ten healthy beagle dogs. Procedures: Dogs were anesthetized and positioned in dorsal recumbency in a 3.0 Tesla MR scanner. Electrocardiogram-triggered dynamic T1-weighted ultrafast gradient echo images of three slices in short-axis orientation of the heart were acquired during breath holds and the first pass of gadolinium contrast. Image acquisition was performed after 4 min infusion of 140 μg/kg/min and 280 μg/kg/min adenosine and, after a washout period, without adenosine, respectively. Images were processed by dividing each slice into 6 radial segments and perfusion analysis was performed from signal intensity-time data. Results: No differences in perfusion parameters were found between segments within any of the slices, but significant differences were found between slices for peak enhancement, accumulated enhancement, and the maximum upslope. In addition, significant differences were found within each slice between data at rest and during adenosine-induced stress for the relative and absolute maximum upslope, relative peak enhancement, time to peak, and accumulated enhancement although inter-individual variation was large and no difference was found between the two stress tests for some parameters. Conclusion and Clinical Relevance: Results of this study showed that rest and stress myocardial perfusion can be assessed using DCE-CMR in dogs using the methods described. Both, adenosine dose and slice appear to affect perfusion parameters in healthy dogs and individual response to adenosine was variable.

In cardiac magnetic resonance imaging (CMR), accurate flow measurements rely on perpendicular plane-alignment with flow direction. For 2D phase contrast (PC) cardiac magnetic resonance measurements, planes have to be defined during the examination of the heart, which is time consuming and error-prone. Collection of flow information of the entire volume of the heart by a 4D flow CMR postpones plane alignment to post-processing. Sampling of such a large amount of data requires acceleration of data acquisition with techniques such as SENSitivity Encoding (k-t SENSE) or Broad-use Linear Acquisition Speed-up Technique (k-t BLAST). Objectives of the study were to compare 4D flow CMR, accelerated with two different acceleration methods with the established 2D PC CMR based on assessment of stroke volume at all four cardiac valves. The values of stroke volume acquired with the 4D flow CMR SENSE did not differ significantly when compared to the 2D PC CMR SENSE at the left side of the heart (aortic and mitral valve). Significant differences between the techniques were seen at the pulmonic and tricuspid valves. Acceleration with k-t BLAST revealed significantly lower values of stroke volume at all cardiac valves, except at the mitral valve.Bei der Herzuntersuchung mittels Magnetresonanztomographie (CMR) muss die Ebene für akkurate Flussmessungen senkrecht zur Blutflussrichtung definiert werden. Im 2D Phasenkontrast (PC) CMR erfolgt die Wahl der Ebene schon während der Untersuchung, was zeitaufwendig und problemanfällig ist. Mit der Messung aller Blutflussinformationen des gesamten Herzens mittels einer 4D flow CMR, verschiebt sich die Wahl der Ebenen in die Nachbearbeitung. Das Sammeln solch grosser Datenmengen erfordert eine Beschleunigung der Messtechnik wie SENSitivity Encoding (k-t SENSE) oder Broad-use Linear Acquisition Speed-up Technique (k-t BLAST). Das Ziel dieser Studie war, die 4D flow CMR, beschleunigt mit zwei verschiedenen Methoden, mit der etablierten 2D PC CMR anhand der Bestimmung des Schlagvolumens über allen vier Herzklappen zu vergleichen. Die Werte der Schlagvolumenmessung für die linke Seite des Herzens (Aorten- und Mitralklappe), durchgeführt anhand der 4D flow CMR k-t SENSE, waren nicht signifikant verschieden im Vergleich zu den Messungen mittels der 2D PC CMR k-t SENSE. An der Pulmonal- und Trikuspidalklappe zeigten sich jedoch signifikante Unterschiede zwischen diesen beiden Techniken. Die Beschleunigung durch k-t-BLAST ergaben generalisiert tiefere Werte des Schlagvolumens an allen Herzklappen, ausser der Mitralklappe.Lors d’examens cardiaques par résonnance magnétique (CMR), le plan pour des mesures de flux précises doit être défini perpendiculairement à la direction de flux sanguin. Dans la CMR en contraste de phases 2D (PC), le choix de ce plan se fait durant l’examen, ce qui prend du temps et peut être sujet à des problèmes. Avec la mesure de toutes les données relatives au flux sanguin dans l’ensemble du coeur au moyen d’un 4D flow CMR, on déplace le moment de ce choix dans la phase de traitement des données. La collecte d’une quantité aussi élevée de données nécessite une accélération de la technique de mesures comme par exemple SENSitivity Encoding (k-t SENSE) ou Broad-use Linear Acquisition Speed-up Technique (k-t BLAST). Le but de cette étude était de comparer la CMR 4D, accélérée avec deux méthodes différentes avec la CMR 2D bien établie, ceci sur la base de la détermination du volume d’éjection systolique au niveau des quatre valvules cardiaques. Les valeurs du volume d’éjection pour le coeur gauche (valvules aortiques et mitrales) obtenues par 4D flow CMR k-t SENSE n’étaient pas significativement différentes de celles obtenues par 2D PC CMR k-t SENSE. Par contre, des différences significatives entre les deux techniques étaient constatées au niveau des valvules pulmonaires et tricuspid. L’accélération par k-t-BLAST donnait de façon générale des valeurs du volume d’élection plus basses au niveau de toutes les valvules, à l’exception de la valvule mitrale.Al momento dell’esame cardiaco mediante risonanza magnetica (CRM) il piano per delle misure accurate del flusso deve venire definito in quanto perpendicolare alla direzione del flusso sanguigno. Già durante la CRM a contrasto di fase 2D (PC), la scelta del piano durante l’esame ha richiesto tempo ed è stata soggetta a problemi. Con la misura di tutte le informazioni del flusso sanguigno in tutto il cuore via una CRM con flusso 4D, la scelta del piano è passata ad un’elaborazione seguente. La raccolta di tali grandi quantità di dati richiede un’accelerazione della tecnica di misura quale SENSitivity Encoding (k-t SENSE) oppure Broad-use Linear Acquisition Speed-up Technique (k-t BLAST). Scopo di questo studio era di paragonare il flusso 4D della CRM, accelerato con due diversi metodi, e con la riconosciuta 2D PC CRM sulla base della determinazione dei volumi di gittata su tutte e quattro le valvole cardiache. I valori della misurazione della gittata nella parte sinistra del cuore (valvola aortica e mitrale), misurati via la CRM a flusso 4D con k-t SENSE erano significativamente differenti rispetto alle misurazioni mediante il k-t SENSE per il 2D PC CRM. Per quel che riguarda la valvola polmonare e la tricuspide si sono riscontrate differenze significative tra queste due tecniche. L’accelerazione via k-t -BLAST ha rivelato in generale valori inferiori del volume di gittata per tutte le valvole cardiache eccetto la valvola mitralica.

Background In cattle, the prognosis of brain abscess is unfavourable and treatment is therefore not recommended. To the knowledge of the authors, there has been no report of successful treatment of a brain abscess in cattle.This report describes the clinical, computed tomographic and postmortem findings in a Holstein–Friesian bull with a hypophyseal abscess. Case report The main clinical findings were generalised ataxia, ptyalism, prolapse of the tongue, dropped jaw, dysphagia, head tilt and unilateral ptosis. Cerebrospinal fluid evaluation revealed 2437 leukocytes/µl and severe pleocytosis. CT examination of the head showed a cavitary lesion consistent with an abscess in the hypophysis. Treatment consisted of gentamicin and flunixin meglumine for 3 days and amoxicillin for 40 days. The neurological signs resolved within 8 days of the start of treatment. The bull was slaughtered 11 months later because of infertility, and a postmortem examination was carried out. Histologically, a mild chronic non suppurative meningoencephalitis restricted to the ventral diencephalon was diagnosed. In addition, there was mild to moderate multifocal chronic lymphoplasmacytic hypophysitis with mild multifocal fibrosis. Conclusions This case report stresses the significance of CT in confirming the clinical and laboratory diagnosis of central nervous system disorders in cattle and for localising brain lesions. Treatment of the brain abscess resulted, with respect to the central nervous disorder, in a successful outcome and was encouraging considering that most cases have an unfavourable prognosis.

Objective To compare the localization and distribution of two different anesthetic fluid volumes around equine cadaver eyes to determine an appropriate volume for a single sub-Tenon's injection in horses. Procedure A single sub-Tenon's injection of 2% lidocaine was performed in 10 equine cadaver heads (20 eyes) using two different volumes (7 mL on one side and 10 mL on the opposite side). The posterior circular distribution of the anesthetic was quantified in sagittal, dorsal, and transverse MRI (T2W-TSE) sequences and evaluated independently by three board-certified radiologists. The distribution of the two fluid volumes was compared via a paired Student's t-test. The interobserver reliability was evaluated via a Kruskal-Wallis test. Results Extension of the injection fluid was observed along the dorsal and temporal quadrants of the globe within the subconjunctival space, the anterior and posterior sub-Tenon's space, and into the muscle sheaths along the extraocular muscles. Accumulation of anesthetic fluid directly surrounding the optic nerve was detected in three of 20 cadaver eyes. Circular distribution of the 7 and 10 mL anesthetic volumes was not significantly different (P = 0.849). More retrograde leakage of the anesthetic was observed using the 10 mL volume. Evaluation of interobserver reliability revealed no significant differences between observers (P = 0.21-0.92). Conclusions Sub-Tenon's anesthesia can have potential as an alternative to retrobulbar anesthesia for ophthalmic surgeries in equines. A 7- to 10-mL injection volume should be appropriate based on the results of this study. The distribution of the anesthetic solution in live tissues, the clinical effects, and the potential for complications will have to be evaluated in vivo.

Aelurostrongylus abstrusus infection is common in endemic areas and may cause severe respiratory clinical signs. Computed tomography (CT) is an important tool to diagnose pulmonary disease, because it allows detection of small lesions and discrimination of superimposed structures. The purpose of this study was to characterize by CT and angiographic CT the pulmonary lesions in six cats before, and 48 and 81 days after inoculation with 100 or 800 A. abstrusus infective larvae. Histological examination of the accessory lung lobe was performed to determine the microscopic, pathomorphologic correlate of the CT findings. The predominant CT lesion consisted of multiple nodules of varying size distributed throughout the lungs, severity depending on infectious dose. The histological correlate of the nodular lesions was multifocal dense granulomatous to mixed inflammatory cell infiltrates, including eosinophils distributed in the parenchyma and obliterating the alveoli. Marked, multifocal, dose-dependent thickening of the bronchi and adjacent interstitial changes blurred the margins of the outer serosal surface of the bronchi and vessels. Histologically, this was due to peribronchial mixed cell inflammation. During the course of infection some of the nodular and peribronchial changes were replaced by areas of ground-glass opacity. In addition to providing detailed depiction of pulmonary lesions resulting from an infectious cause and clearly defining lesions with respect to time and severity of infection, CT allowed quantitative assessment of bronchial thickness and lymph node size during the course of disease. Findings indicated that CT characteristics of this disease are consistent with pathologic findings.

A 2-month-old female goat was presented for depressed mental status and multifocal central neurologic signs 3 weeks after hot-iron disbudding. Conventional magnetic resonance imaging (MRI) findings included a large intra axial mass in the left frontal lobe that was T2 hyperintense and T1 hypointense centrally with a contrast-enhancing peripheral capsule and perilesional T2 hyperintensity. A restrictive pattern was present in diffusion-weighted imaging. Magnetic resonance spectroscopy demonstrated an increased amount of succinate, acetate, amino acids, lipids; minimal amounts of lactate; and decreased amounts of N-acetyl aspartate and choline. A cerebral abscess due to Trueperella pyogenes was confirmed from necropsy and tissue culture.

A 2.5-year-old female guinea pig (Cavia porcellus) presented for reduced appetite, signs of abdominal pain, increased respiratory effort, and a distended abdomen. Cholelithiasis was diagnosed by radiography and ultrasonography. The cholelith was surgically removed using cholecystectomy. After surgery the animal recovered quickly and clinical signs resolved. Stone analysis revealed a composition of 20% weddellite (calcium oxalate dehydrate) and 80% apatite (calcium phosphate). Histologic examination of the gallbladder revealed no significant pathologic changes. Three months after the surgical procedure the patient continued to do well and diagnostic imaging revealed no abnormalities. Surgical therapy was straightforward and effective.

OBJECTIVE To assess the effects of 3 contrast medium injection techniques on attenuation values for canine adrenal glands during contrast-enhanced CT. ANIMALS 9 healthy Beagles. PROCEDURES 3 protocols were evaluated in a randomized cross-over design study: 700 mg of iodine/kg at a constant injection rate over 20 seconds (full-dose constant rate), the same dose at a rate following an exponential decay curve over 20 seconds (full-dose decelerated rate), and 350 mg of iodine/kg at a constant injection rate over 10 seconds (half-dose constant rate). Multislice CT images were obtained before and at predetermined time points after the start of contrast medium injection. RESULTS Median peak attenuation values were 129, 133, and 87 Hounsfield units with the full-dose constant rate, full-dose decelerated rate, and half-dose constant rate injection protocols, respectively. Peak attenuation differed significantly between the full-dose constant rate and half-dose constant rate injection protocols and between the full-dose decelerated rate and half-dose constant rate injection protocols. Median time to peak attenuation did not differ significantly among injection methods and was 30, 23, and 15 seconds for the full-dose constant rate, full-dose decelerated rate, and half-dose constant rate injections, respectively. CONCLUSIONS AND CLINICAL RELEVANCE The dose of contrast medium and the timing of postinjection CT scanning were main determinants of peak attenuation for adrenal glands in healthy dogs; effects of the 3 injection protocols on attenuation were minor. The exponentially decelerated injection method was subjectively complex. A constant injection protocol delivering 700 mg of iodine/kg over 20 seconds, with scans obtained approximately 30 seconds after starting contrast medium injection, provided images with maximum adrenal gland attenuation values. (Am J Vet Res 2016;77:144–150)

Objective—To determine angles of insertion for laminar vertebral fixation of L1 and L2 by use of a locking plate in dogs and to confirm screw placement by use of computed tomography (CT). Sample—Vertebral specimens harvested from 8 canine cadavers. Procedures—The point of insertion and minimum and maximum insertion angles for laminar and facet screws for laminar vertebral stabilization were determined by use of CT. A precontoured locking plate was then placed by use of 1 locking screw in the lamina of each lumbar vertebra and 1 nonlocking screw in the facet joint. The position and angle of the screws were examined by use of CT, and penetration into the vertebral canal was recorded. Results—Mean ± SD insertion angles for L1 and L2 were 18 ± 4° and 21 ± 5° toward the vertebral canal and 11 ± 4.4° and 10 ± 3° in a dorsal direction, respectively. Insertion angles for the facet joint were between 24 ± 4° ventrally and 12 ± 2° dorsally. Insertion of the screw did not penetrate the vertebral canal for 23 of 24 (96%) screws. For 23 of 24 inserted screws, the previously determined angle was maintained and purchase of bone and cortices was satisfactory. Conclusions and Clinical Relevance—Placement of laminar and facet screws in canine vertebrae was possible and can be performed safely if angles of insertion determined pre-operatively via CT are maintained.

Our goal was to investigate the effect of contrast medium injection duration on pulmonary artery peak enhancement and time to peak enhancement. Fourteen dogs were allocated into one of seven predefined weight categories, each category contained two dogs. Dogs in each weight category were assigned to group A or B. Animals in each group received a different contrast medium injection protocol. In group A, a fixed injection rate of 5 ml/s was used. In group B, the contrast injection rate was calculated as follows: flow rate= contrast volume/scan duration + 10s. Time to peak enhancement and peak enhancement of the main left and right pulmonary arteries were measured on single-level, dynamic CT images for a fixed time of 30s. Rank correlation (Spearman's) coefficients between injection duration and time to peak enhancement and between body weight and peak enhancement were calculated. For group A, there was a significant negative correlation between peak enhancement and weight (r = -0.94; P = 0.005), while for group B, there was no significant correlation (r = -0.64 and P = 0.18). There was a significant correlation between time to peak enhancement and injection duration in both groups (group A: r = 0.99; P = 0.006 and group B: r = 0.85; P = 0.02). In conclusion, injection duration is a key feature in a CT angiography injection protocol. A protocol with an injection duration adjusted to the scan duration seems to be particularly suitable for veterinary applications where a population with great weight variability is studied.

To characterize the computed tomography (CT) features of thoracic lesions caused by infection with Angiostrongylus vasorum, pre- and postcontrast CT was performed in six experimentally infected Beagles 13 weeks postinoculation and in four of these 9 weeks postchemotherapy. Findings were compared with survey radiographs and necropsy findings. A multicentric bronchoalveolar pattern more pronounced at the lung periphery was present radiographically. On CT, the predominant abnormality underlying this alveolar pattern was multiple large nodules merging to areas of consolidation, and containing air bronchograms of varying extent. These nodular changes corresponded to histopathologic granulomata, consisting mainly of macrophages, multinucleated giant cells, and lymphocytes that had accumulated around larvae and eggs. Morphologically, no bronchial changes were observed on CT or histologically. Quantitatively, however, on CT there was evidence of bronchial thickening at 13 weeks postinoculation and mild very peripheral bronchiectasia 9 weeks postchemotherapy. Regional lymph nodes were enlarged after infection, and smaller after treatment. On postcontrast CT, several suspicious intraluminal filling defects suggestive of thrombosis were found; however, the tortuosity of some pulmonary arteries seen radiographically was not present in CT images. After treatment, the consolidations and large nodules had almost completely disappeared. A remaining radiographic interstitial pattern was characterized on CT as ground-glass opacifications, subpleural interstitial thickening, subpleural lines, and interface signs. These interstitial changes reflected fibrosis as documented histopathologically. CT allowed very detailed and accurate characterization of pulmonary parenchymal lesions, bronchi, and lymphnodes and closely reflected histopathological changes.

Summary External lipoma is a benign subcutaneous and submucosal tumour and usually is of little clinical concern. Occasionally, the lipoma may invade muscles, the infiltrating lipoma. Complete surgical excision is mandatory because of the infiltrative nature and potentially high incidence of recurrence after inadequate surgery. In order to assess the extension of the tumour accurately, computed tomographic examination is indicated.

Background: Dogs experimentally inoculated with Angiostrongylus vasorum develop severe pulmonary parenchymal lesions and arterial thrombosis at the time of patency. Hypothesis: A. vasorum-induced thrombosis results in arterial hypoxemia, pulmonary hypertension (PH), and altered cardiac morphology and function. Animals: Six healthy Beagles experimentally inoculated with A. vasorum. Methods: Thoracic radiographs and arterial blood gas analyses were performed 8 and 13 weeks postinoculation (wpi) and 9 weeks posttherapy (wpt). Echocardiography was done before and 2, 5, 8, 13 wpi and 9 wpt. Invasive pulmonary artery pressure (PAP) measurements were obtained 8 wpi. Two untreated dogs were necropsied 13 wpi and 4 treated dogs 9 wpt. Results: All dogs had patent infections at 7 wpi and clinical respiratory signs at 8 wpi. Moderate hypoxemia (median PaO2 of 73 and 74 mmHg) present at 8 and 13 wpi had resolved by 9 wpt. Echocardiographically, no evidence of PH and no abnormalities in cardiac size and function were discernible at any time point. PAP invasively measured at 8 wpi was not different from that of control dogs. Severe radiographic pulmonary parenchymal and suspected thrombotic lesions at 13 wpi were corroborated by necropsy. Most histopathologic changes had resolved at 9 wpt, but focal inflammatory, thrombotic, and fibrotic changes still were present in all dogs. Conclusion: In experimentally infected Beagles, pulmonary and vascular changes induced by A. vasorum are reflected by marked radiographic changes and arterial hypoxemia. These did not result in PH and echocardiographic changes in cardiac size and function.

A 5-year-old, female golden eagle (Aquila chrysaetos) was admitted with tetraplegia that progressed to a nonambulatory, spastic tetraparesis after a few days of treatment. Clinical and radiologic examinations, including radiography, computed tomography scan, and myelography, were indicative of neoplasia involving a spinal nerve root. Postmortem magnetic resonance imaging and necropsy findings confirmed the diagnosis of a peripheral nerve sheath neoplasia, not, to our knowledge, previously reported in a raptor.

Case Description—A 3-year-old French Bulldog was evaluated because of acute signs of back pain and spastic paraparesis. Clinical Findings—Neuroanatomic localization indicated a lesion in the T3-L3 spinal cord segment. Magnetic resonance imaging revealed extradural spinal cord compression at the ventral right aspect of the intervertebral disk space L3–4. On the basis of these findings, a diagnosis of sequestrated Hansen type 1 disk extrusion without extradural hemorrhage was made. Treatment and Outcome—The dog was treated conservatively with cage rest, restricted exercise on a leash, and NSAIDs. Results of follow-up examination 5 weeks later indicated complete resolution of clinical signs, and results of repeated MRI indicated a 69% reduction in the volume of the herniated disk material. Clinical Relevance—Findings for the dog of this report indicated spinal cord compression attributable to extruded intervertebral disk material resolved. Functional improvements in dogs with such problems may be partly attributable to spontaneous regression of intervertebral disk extrusions.

Objective—To investigate clinical use of proton magnetic resonance spectroscopy (1H MRS) and to compare metabolic brain bioprofiles of dogs with and without hepatic encephalopathy. Animals—6 dogs with hepatic encephalopathy and 12 control dogs. Procedures—Conventional MRI and single-voxel 1H MRS were performed with a 3-T magnet. Images for routine MRI planes and sequences were obtained. Single-voxel 1H MRS was performed with a point-resolved sequence with a short echo time (35 milliseconds) and voxel of interest placement at the level of the basal ganglia. Metabolites of interest included the glutamine-glutamate complex (sum quantification of glutamate and glutamine), myoinositol, N-acetyl aspartate, total choline, and creatine. Data were analyzed with postprocessing fitting algorithm software, and metabolite concentration relative to water and ratios with creatine as the reference metabolite were calculated. Results—Compared with control dogs, dogs with hepatic encephalopathy had specific changes, which included significantly higher concentration relative to water of the glutamine-glutamate complex and significantly lower concentration of myoinositol. Choline and N-acetyl aspartate concentrations were also slightly lower in dogs with hepatic encephalopathy than in control dogs. No differences in creatine concentration were detected between groups. Conclusions and Clinical Relevance—1H MRS aided in the diagnosis of hepatic encephalopathy in dogs, and findings supported the assumption that ammonia is a neurotoxin that manifests via glutamine-glutamate complex derangements. Use of 1H MRS may provide clinically relevant information in patients with subclinical hepatic encephalopathy, equivocal results of bile acids tests, and equivocal ammonia concentrations or may be helpful in monitoring efficacy of medical management.

Border disease (BD) virus belongs to the genus Pestivirus together with bovine viral diarrhoea virus (BVDV) genotypes 1 and 2 and classical swine fever virus (Pletnev and others 2011). The pathogenicity of BD virus for bovine fetuses has been recognised early (Gibbons and others 1974), but only since 1994 has renewed attention been drawn to the mutual transmission between species (Carlsson and Belak 1994). Transmission of BD virus from persistently infected sheep to cattle was demonstrated (Krametter-Frotscher and others 2008, 2009, Reichle 2009, Braun and others 2013, 2014). Nevertheless, reports on clinical disease in cattle caused by BD virus are rare (Cranwell and others 2007, Krametter-Frotscher and others 2010, McFadden and others 2012). The present report describes the radiological and pathological findings in three calves (Table 1) persistently infected with BD virus. Persistent infection with pestivirus was suspected based on an initial positive ear notch sample taken within 1 week after birth on the three different farms of origin and tested in private laboratories by ELISA or RT-PCR. This was later confirmed in our lab using real-time RT-PCR and serology on blood samples collected independently on at least two occasions, a minimum of 17 days apart. Thus, all calves were strongly positive for viral RNA and negative for serum antibodies against pestiviruses at the latest time point analysed, that is, at the age of 2, 8 and 15 months after birth for animals 1, 2 and 3, respectively. This identifies all three calves as persistently infected with a pestivirus. Genotyping based on the sequence within the 5′ untranslated region of the pestiviral genome (Bachofen and others 2008) revealed that calves were persistently infected with BD virus ‘Switzerland’ (Peterhans and others 2010). This was corroborated by the fact that (i) the mother animals of all three calves possessed …