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1. Synthesis and Biodistribution of 99mTc-Labeled PLGA Nanoparticles by Microfluidic Technique

Author

Michela Varani, Giuseppe Campagna, Valeria Bentivoglio, Matteo Serafinelli, Maria Luisa Martini, Filippo Galli, and Alberto Signore

Subjects
radiolabeled nanoparticles, poly (lactic-co-glycolic acid) (PLGA), nuclear medicine, microfluidics, Pharmacy and materia medica, RS1-441
Abstract

The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch reproducibility and tailored-made size of NPs. Eighteen different formulations were tested and characterized for particle size, zeta potential, polydispersity index, labeling efficiency, and in vitro stability. Overall, physical characterization by dynamic light scattering (DLS) showed an increase in particle size after radiolabeling probably due to the incorporation of the isotope into the PLGA-NPs shell. NPs of 60 nm (obtained by 5:1 PVA:PLGA ratio and 15 mL/min TFR with 99mTc included in PVA) had high labeling efficiency (94.20 ± 5.83%) and >80% stability after 24 h and showed optimal biodistribution in BALB/c mice. In conclusion, we confirmed the possibility of radiolabeling NPs with 99mTc using the microfluidics and provide best formulation for tumor targeting studies.

Published
2021
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2. Synthesis and biodistribution of99mTc-labeled PLGA nanoparticles by microfluidic technique

Author

Matteo Serafinelli, Alberto Signore, Maria Luisa Martini, Filippo Galli, Valeria Bentivoglio, Michela Varani, and Giuseppe Campagna

Subjects
Biodistribution, Chromatography, microfluidics, nuclear medicine, poly (lactic-co-glycolic acid) (PLGA), radiolabeled nanoparticles, Dispersity, Microfluidics, Pharmaceutical Science, Fluorescence, RS1-441, PLGA, chemistry.chemical_compound, Pharmacy and materia medica, chemistry, Dynamic light scattering, Zeta potential, poly (lactic-co-glycolic acid) (plga), Particle size
Abstract

The aim of present study was to develop radiolabeled NPs to overcome the limitations of fluorescence with theranostic potential. Synthesis of PLGA-NPs loaded with technetium-99m was based on a Dean-Vortex-Bifurcation Mixer (DVBM) using an innovative microfluidic technique with high batch-to-batch reproducibility and tailored-made size of NPs. Eighteen different formulations were tested and characterized for particle size, zeta potential, polydispersity index, labeling efficiency, and in vitro stability. Overall, physical characterization by dynamic light scattering (DLS) showed an increase in particle size after radiolabeling probably due to the incorporation of the isotope into the PLGA-NPs shell. NPs of 60 nm (obtained by 5:1 PVA:PLGA ratio and 15 mL/min TFR with 99mTc included in PVA) had high labeling efficiency (94.20 ± 5.83%) and &gt, 80% stability after 24 h and showed optimal biodistribution in BALB/c mice. In conclusion, we confirmed the possibility of radiolabeling NPs with 99mTc using the microfluidics and provide best formulation for tumor targeting studies.

Published
2021

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