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244 results on '"Matsuzaki, Shigenobu"'

10. Bacteriophage-Conjugated Fluorescent Nanoemulsion as a Novel Optical Probe for Highly Selective Bacterial Detection

Author

Sekida, Shin-ya, Chisaka, Takatoshi, Uchiyama, Jumpei, Takemura-Uchiyama, Iyo, Matsuzaki, Shigenobu, Niko, Yosuke, Hadano, Shingo, and Watanabe, Shigeru

Abstract

Infectious disease mortality has decreased due to effective drugs and healthcare. However, global health remains threatened by infectious diseases. New methods of rapid and accurate bacterial detection have attracted considerable attention. Fluorescence detection of whole bacterial cells offers high sensitivity, quantitative analysis, and simple operation. A highly fluorescent bioconjugated probe improves sensitivity and selectivity. This study presents a novel, bright fluorescent probe comprising a bacteriophage and a fluorescent nanoemulsion (fNE) as biorecognition and signal transduction elements, respectively. We demonstrate that fluorescence microscopy imaging using the S. aureus-specific phage, S13′-fNE (phage-fNE), detects S. aureusin the presence of E. colior S. pseudintermedius, another closely related Staphylococci, in a highly selective manner. Furthermore, fNEs with high dye loadings exhibit considerably greater brightness compared to the fluorescent dye alone, making them suitable for sensitive fluorescence imaging. Phage-fNEs can quantitatively detect S. aureusat 104–108colony-forming units per milliliter (CFU mL−1), with a limit of detection of 8 × 104CFU mL−1. This result is comparable to the lowest value achieved by microscopic bacterial detection, with no preconcentration or enzymatic signal enhancement methods used. Bioconjugated fNEs open new avenues for highly selective and sensitive fluorescent detection of bacteria.This study presents a novel, bright fluorescent probe comprising a bacteriophage and a fluorescent nanoemulsion (fNE) as biorecognition and signal transduction elements, respectively. We demonstrate that fluorescence microscopy imaging using the S. aureus-specific phage, S13′-fNE (phage-fNE), detects S. aureusin the presence of E. colior S. pseudintermedius, another closely related Staphylococci, in a highly selective manner.

Published
2023
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13. Phylogenic analysis of new viral cluster of large phages with unusual DNA genomes containing uracil in place of thymine in gene-sharing network, using phages S6 and PBS1 and relevant uncultured phages derived from sewage metagenomics

Author

Uchiyama, Jumpei, primary, Takemura-Uchiyama, Iyo, additional, Gotoh, Kazuyoshi, additional, Kato, Shin-ichiro, additional, Sakaguchi, Yoshihiko, additional, Murakami, Hironobu, additional, Fukuyama, Tomoki, additional, Kaneki, Mao, additional, Matsushita, Osamu, additional, and Matsuzaki, Shigenobu, additional

Published
2022
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14. Phylogenic analysis of new viral cluster of large phages with unusual DNA genomes containing uracil in place of thymine in gene-sharing network, using phages S6 and PBS1 and relevant uncultured phages derived from sewage metagenomics

Author

Uchiyama, Jumpei, Takemura-Uchiyama, Iyo, Gotoh, Kazuyoshi, Kato, Shin-ichiro, Sakaguchi, Yoshihiko, Murakami, Hironobu, Fukuyama, Tomoki, Kaneki, Mao, Matsushita, Osamu, Matsuzaki, Shigenobu, Uchiyama, Jumpei, Takemura-Uchiyama, Iyo, Gotoh, Kazuyoshi, Kato, Shin-ichiro, Sakaguchi, Yoshihiko, Murakami, Hironobu, Fukuyama, Tomoki, Kaneki, Mao, Matsushita, Osamu, and Matsuzaki, Shigenobu

Abstract

Bacteriophages (phages) are the most diverse and abundant life-form on Earth. Jumbophages are phages with double-stranded DNA genomes longer than 200 kbp. Among these, some jumbophages with uracil in place of thymine as a nucleic acid base, which we have tentatively termed "dU jumbophages" in this study, have been reported. Because the dU jumbophages are considered to be a living fossil from the RNA world, the evolutionary traits of dU jumbophages are of interest. In this study, we examined the phylogeny of dU jumbophages. First, tBLASTx analysis of newly sequenced dU jumbophages such as Bacillus phage PBS1 and previously isolated Staphylococcus phage S6 showed similarity to the other dU jumbophages. Second, we detected the two partial genome sequences of uncultured phages possibly relevant to dU jumbophages, scaffold_002 and scaffold_007, from wastewater metagenomics. Third, according to the gene-sharing network analysis, the dU jumbophages, including phages PBS1 and S6, and uncultured phage scaffold_002 formed a cluster, which suggested a new viral subfamily/family. Finally, analyses of the phylogenetic relationship with other phages showed that the dU jumbophage cluster, which had two clades of phages infecting Gram-negative and Gram-positive bacteria, diverged from the single ancestral phage. These findings together with previous reports may imply that dU jumbophages evolved from the same origin before divergence of Gram-negative and Gram-positive bacteria.

Published
2022

29. In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis

Author

Kishimoto, Tatsuma, primary, Ishida, Waka, additional, Nasukawa, Tadahiro, additional, Ujihara, Takako, additional, Nakajima, Isana, additional, Suzuki, Takashi, additional, Uchiyama, Jumpei, additional, Todokoro, Daisuke, additional, Daibata, Masanori, additional, Fukushima, Atsuki, additional, Matsuzaki, Shigenobu, additional, and Fukuda, Ken, additional

Published
2021
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32. Therapeutic Effects of Intravitreously Administered Bacteriophage in a Mouse Model of Endophthalmitis Caused by Vancomycin-Sensitive or -Resistant Enterococcus faecalis

Author

Kishimoto, Tatsuma, primary, Ishida, Waka, additional, Fukuda, Ken, additional, Nakajima, Isana, additional, Suzuki, Takashi, additional, Uchiyama, Jumpei, additional, Matsuzaki, Shigenobu, additional, Todokoro, Daisuke, additional, Daibata, Masanori, additional, and Fukushima, Atsuki, additional

Published
2019
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34. Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage (phi)MR11

Author

Rashel, Mohammad, Uchiyama, Jumpei, Ujihara, Takako, Uehara, Yoshio, Kuramoto, Shu, Sugihara, Shigeyoshi, Yagyu, Ken-Ichi, Muraoka, Asako, Sugai, Motoyuki, Hiramatsu, Keiichi, Honke, Koichi, and Matsuzaki, Shigenobu

Subjects
Staphylococcus aureus -- Research, Clone cells -- Usage, Bacteriophages -- Health aspects, Bacteriophages -- Genetic aspects, Vancomycin -- Dosage and administration, Health
Published
2007

36. Experimental protection of mice against lethal Staphylococcus aureus infection by novel bacteriophage [phi]MR11. (Major Article)

Author

Matsuzaki, Shigenobu, Yasuda, Masaharu, Nishikawa, Hiroshi, Kuroda, Masayuki, Ujihara, Takako, Shuin, Taro, Shen, Yuan, Jin, Zhe, Fujimoto, Shigeyoshi, Nasimuzzaman, M.D., Wakiguchi, Hiroshi, Sugihara, Shigeyoshi, Sugiura, Tetsuro, Koda, Shigeki, Muraoka, Asako, and Imai, Shosuke

Subjects
Staphylococcus -- Research, Staphylococcus -- Evaluation, Infection -- Research, Infection -- Evaluation, Mice as laboratory animals -- Usage, Health
Published
2003

38. Highly specific and sensitive detection of bacteria by dark-field light-scattering imaging based on bacteriophage-modified magnetoplasmonic nanoparticles

Author

Imai, Masashi, Uchiyama, Jumpei, Takemura-Uchiyama, Iyo, Matsuzaki, Shigenobu, Niko, Yosuke, Hadano, Shingo, and Watanabe, Shigeru

Abstract

Bacterial infections pose significant global health concerns, necessitating precise and sensitive detection methods. This study introduces a multifunctional probe for bacterial detection. Bifunctional magnetoplasmonic nanoparticles (NPs) serve a dual purpose as carriers for magnetic separation (MS) and enhancers for light scattering of the target bacteria. We achieved exceptional selectivity at the bacterial species level by bioconjugating them with bacteriophages (phages). Optimal phage coverage was carefully determined to enhance the capture efficiency of the target bacterial cells. Additionally, we identified the ideal mixing ratios of the phage probe to bacteria, ensuring efficient MS and high light-scattering intensity. The use of the phage probe enabled the successful separation of captured target bacteria from other bacterial strains in the sample mixture. Bacterial detection was accomplished through dark-field light-scattering imaging, eliminating the need for additional labeling with other probes. This method showed a remarkable sensitivity, achieving a detection limit of ∼102colony-forming units ml−1. This value is 2 orders of magnitude lower than that achieved using our previously reported technique using monofunctional plasmonic NPs. These phage probes hold promise for healthcare, biotech, and environmental monitoring owing to their adaptability to diverse bacterial species.Graphical AbstractThis study introduces a novel multifunctional probe for selective bacterial detection using bioconjugated magnetoplasmonic nanoparticles (MagPlas NPs). Employing phage-modified NPs enhances bacterial species specificity through efficient magnetic separation and light scattering. The method achieves high sensitivity (limit of detection: 102colony-forming units ml−1), promising versatile applications in healthcare, biotech, and environmental monitoring.

Published
2024
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39. Potential Application of Bacteriophages in Enrichment Culture for Improved Prenatal Streptococcus agalactiae Screening

Author

Uchiyama, Jumpei, primary, Matsui, Hidehito, additional, Murakami, Hironobu, additional, Kato, Shin-ichiro, additional, Watanabe, Naoki, additional, Nasukawa, Tadahiro, additional, Mizukami, Keijiro, additional, Ogata, Masaya, additional, Sakaguchi, Masahiro, additional, Matsuzaki, Shigenobu, additional, and Hanaki, Hideaki, additional

Published
2018
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40. Potential Application of Bacteriophages in Enrichment Culture for Improved PrenatalStreptococcus agalactiaeScreening

Author

Uchiyama, Jumpei, primary, Matsui, Hidehito, additional, Murakami, Hironobu, additional, Kato, Shini-chiro, additional, Watanabe, Naoki, additional, Nasukawa, Tadahiro, additional, Mizukami, Keijiro, additional, Ogata, Masaya, additional, Sakaguchi, Masahiro, additional, Matsuzaki, Shigenobu, additional, and Hanaki, Hideaki, additional

Published
2018
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45. Adsorption of Staphylococcus viruses S13′ and S24-1 on Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids

Author

Uchiyama, Jumpei, primary, Taniguchi, Maya, additional, Kurokawa, Kenji, additional, Takemura-Uchiyama, Iyo, additional, Ujihara, Takako, additional, Shimakura, Hidekatsu, additional, Sakaguchi, Yoshihiko, additional, Murakami, Hironobu, additional, Sakaguchi, Masahiro, additional, and Matsuzaki, Shigenobu, additional

Published
2017
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48. A Primary Model of Commuting Travel Mode Choice by Taking Account of Disutility of Commutation

Author

Li, Qiang, Ooeda, Yoshinao, Sumi, Tomonori, and Matsuzaki, Shigenobu

Subjects
Person Trip (PT) survey, Commutation activity, Disutility analysis, Commuting travel mode choice, Mass transit systems
Abstract

To improve the services of mass transit systems so as to attract additional travelers switching from private cars, it is necessary to grasp the travel mode choice behaviors of the workers. This paper attempted to propose a travel mode choice model for the workers commuting by mass transit systems by taking account of disutility derived from both processes of going to work and returning home. First, commutation activity was defined as a complete process that starts on departure time from home in the morning and ends at home-returning time in the evening. Meanwhile, disutility involved in going to work process and returning home process were formulated based on the feature that commutation is conditional on temporal constraints of the appointed work start time and end time. Then, a travel mode choice model synthetically considering both processes of going to work and returning home was proposed and calibrated by applying Person Trip survey data in Fukuoka City. The result indicated that home-returning process actually affects the travel mode choices even if its influence is less than that of going to work process. The model has good fitness in representing travel mode choice behaviors of the workers using mass transit systems.

Published
2003

49. High load of Merkel cell polyomavirus DNA detected in the normal skin of Japanese patients with Merkel cell carcinoma

Author

Hashida, Yumiko, primary, Nakajima, Kimiko, additional, Nakajima, Hideki, additional, Shiga, Takeo, additional, Tanaka, Moe, additional, Murakami, Masanao, additional, Matsuzaki, Shigenobu, additional, Naganuma, Seiji, additional, Kuroda, Naoki, additional, Seki, Yasutaka, additional, Katano, Harutaka, additional, Sano, Shigetoshi, additional, and Daibata, Masanori, additional

Published
2016
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50. Analyses of Short-Term Antagonistic Evolution of Pseudomonas aeruginosa Strain PAO1 and Phage KPP22 (Myoviridae Family, PB1-Like Virus Genus)

Author

Uchiyama, Jumpei, primary, Suzuki, Masato, additional, Nishifuji, Koji, additional, Kato, Shin-ichiro, additional, Miyata, Reina, additional, Nasukawa, Tadahiro, additional, Yamaguchi, Kotoe, additional, Takemura-Uchiyama, Iyo, additional, Ujihara, Takako, additional, Shimakura, Hidekatsu, additional, Murakami, Hironobu, additional, Okamoto, Noriaki, additional, Sakaguchi, Yoshihiko, additional, Shibayama, Keigo, additional, Sakaguchi, Masahiro, additional, and Matsuzaki, Shigenobu, additional

Published
2016
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