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14 results on '"Matsumoto, Shoma"'

4. Wnt signaling activation confers a syncytiotrophoblast progenitor state on trophoblast stem cells of cynomolgus monkey†

Author

Matsumoto, Shoma and Tanaka, Satoshi

Abstract

Trophoblast stem cells, derived from the trophectoderm of the blastocyst, are used as an in vitro model to reveal the mechanisms underlying placentation in mammals. In humans, suitable culture conditions for trophoblast stem cell derivation have recently been established. The established human trophoblast stem cells differentiate efficiently toward two trophoblast subtypes: syncytiotrophoblasts and extravillous trophoblasts. However, the efficiency of differentiation is lower in macaque trophoblast stem cells than in human trophoblast stem cells. Here, we demonstrate that the activation of Wnt signaling downregulated the expression of inhibitory G protein and induced trophoblastic lineage switching to the syncytiotrophoblast progenitor state. The treatment of macaque trophoblast stem cells with a GSK-3 inhibitor, CHIR99021, upregulated syncytiotrophoblast progenitor markers and enhanced proliferation. Under the Wnt signaling–activated conditions, macaque trophoblast stem cells effectively differentiated to syncytiotrophoblasts upon dibutyryl cyclic AMP (dbcAMP) and forskolin treatment. RNA-seq analyses revealed the downregulation of inhibitory G protein, which may make macaque trophoblast stem cells responsive to forskolin. Interestingly, this lineage switching appeared to be reversible as the macaque trophoblast stem cells lost responsiveness to forskolin upon the removal of CHIR99021. The ability to regulate the direction of macaque trophoblast stem cell differentiation would be advantageous in elucidating the mechanisms underlying placentation in non-human primates.The activation of Wnt signaling in macaque trophoblast stem cell lines potentiated differentiation toward syncytiotrophoblast cell fate.Graphical Abstract

Published
2024
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5. The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins

Author

Jargalsaikhan, Bat-Erdene, primary, Muto, Masanaga, additional, Been, Youngeun, additional, Matsumoto, Shoma, additional, Okamura, Eiichi, additional, Takahashi, Tadanobu, additional, Narimichi, Yutaka, additional, Kurebayashi, Yuuki, additional, Takeuchi, Hideyuki, additional, Shinohara, Takashi, additional, Yamamoto, Ryo, additional, and Ema, Masatsugu, additional

Published
2024
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6. Null mutation of exocyst complex component 3-like does not affect vascular development in mice

Author

Takashima, Satsuki, primary, Okamura, Eiichi, additional, Ichiyama, Yusuke, additional, Nishi, Kiyoto, additional, Shimizu, Akio, additional, Watanabe, Chisato, additional, Muto, Masanaga, additional, Matsumoto, Shoma, additional, Tsukiyama-Fujii, Setsuko, additional, Tsukiyama, Tomoyuki, additional, Ogita, Hisakazu, additional, Nishi, Eiichiro, additional, Ohji, Masahito, additional, Sugiyama, Fumihiro, additional, Takahashi, Satoru, additional, Mizuno, Seiya, additional, Mizutani, Ken-ichi, additional, and Ema, Masatsugu, additional

Published
2024
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7. Highly efficient transgenic mouse production using piggyBac and its application to rapid phenotyping at the founder generation

Author

Okamura, Eiichi, primary, Mizuno, Seiya, additional, Matsumoto, Shoma, additional, Murata, Kazuya, additional, Tanimoto, Yoko, additional, Tra, Dinh Thi Huong, additional, Suzuki, Hayate, additional, Kang, Woojin, additional, Ema, Tomoka, additional, Morimoto, Kento, additional, Kato, Kanako, additional, Matsumoto, Tomoko, additional, Masuyama, Nanami, additional, Kijima, Yusuke, additional, Morimura, Toshifumi, additional, Sugiyama, Fumihiro, additional, Takahashi, Satoru, additional, Mizutani, Eiji, additional, Woltjen, Knut, additional, Yachie, Nozomu, additional, and Ema, Masatsugu, additional

Published
2023
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10. Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

Author

Watanabe, Chisato, primary, Shibuya, Hirotoshi, additional, Ichiyama, Yusuke, additional, Okamura, Eiichi, additional, Tsukiyama-Fujii, Setsuko, additional, Tsukiyama, Tomoyuki, additional, Matsumoto, Shoma, additional, Matsushita, Jun, additional, Azami, Takuya, additional, Kubota, Yoshiaki, additional, Ohji, Masahito, additional, Sugiyama, Fumihiro, additional, Takahashi, Satoru, additional, Mizuno, Seiya, additional, Tamura, Masaru, additional, Mizutani, Ken-ichi, additional, and Ema, Masatsugu, additional

Published
2022
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12. Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts

Author

40648424, Matsumoto, Shoma, Porter, Christopher J., Ogasawara, Naomi, Iwatani, Chizuru, Tsuchiya, Hideaki, Seita, Yasunari, Chang, Yu-Wei, Okamoto, Ikuhiro, Saitou, Mitinori, Ema, Masatsugu, Perkins, Theodore J., Stanford, William L., Tanaka, Satoshi, 40648424, Matsumoto, Shoma, Porter, Christopher J., Ogasawara, Naomi, Iwatani, Chizuru, Tsuchiya, Hideaki, Seita, Yasunari, Chang, Yu-Wei, Okamoto, Ikuhiro, Saitou, Mitinori, Ema, Masatsugu, Perkins, Theodore J., Stanford, William L., and Tanaka, Satoshi

Abstract

The placenta forms a maternal-fetal junction that supports many physiological functions such as the supply of nutrition and exchange of gases and wastes. Establishing an in vitro culture model of human and non-human primate trophoblast stem/progenitor cells is important for investigating the process of early placental development and trophoblast differentiation. In this study, we have established five trophoblast stem cell (TSC) lines from cynomolgus monkey blastocysts, named macTSC #1-5. Fibroblast growth factor 4 (FGF4) enhanced proliferation of macTSCs, while other exogenous factors were not required to maintain their undifferentiated state. macTSCs showed a trophoblastic gene expression profile and trophoblast-like DNA methylation status and also exhibited differentiation capacity towards invasive trophoblast cells and multinucleated syncytia. In a xenogeneic chimera assay, these stem cells contributed to trophectoderm (TE) development in the chimeric blastocysts. macTSC are the first primate trophoblast cell lines whose proliferation is promoted by FGF4. These cell lines provide a valuable in vitro culture model to analyze the similarities and differences in placental development between human and non-human primates.

Published
2020

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