1. Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: immunochemical characterization by fusion protein antibodies.
- Author
-
Sinha D, Bonner TI, Bhat NR, and Matsuda LA
- Subjects
- Animals, Animals, Newborn, Antibody Specificity, Brain cytology, Brain Chemistry immunology, CHO Cells, Cannabinoids genetics, Cannabinoids immunology, Cricetinae, Female, GTP-Binding Proteins immunology, Gene Expression, Macrophages chemistry, Macrophages immunology, Microglia immunology, Rabbits, Rats, Receptors, Cannabinoid, Receptors, Drug analysis, Recombinant Fusion Proteins immunology, Schistosoma japonicum, Brain immunology, Microglia chemistry, Receptors, Drug genetics, Receptors, Drug immunology
- Abstract
Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CB1 cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain.
- Published
- 1998
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