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The COVID-19 pandemic forced universities into emergency remote learning. For universities situated in a country where internet connectivity prices are high, network coverage is low, and most students live in rural areas, this presented a unique set of problems. One of the issues is that traditional orientations were face-to-face for first-time entering students (FTENs) at universities, and they had to be moved online due to lockdown restrictions and the desire to save the academic year. For the university concerned, this presented challenges in accessing students due to their low digital literacy skills and infrastructure limitations. The study employed a quantitative exploratory research approach to assess whether WhatsApp, a low-cost communication tool offering features like affordable data bundles, group creation, and accessibility, could facilitate student engagement and support during the COVID-19 lockdown at a rural university campus. The aim was to investigate if utilizing WhatsApp could foster a sense of belonging among students, potentially mitigating dropout rates and preventing students from falling behind. The hypothesis posited a positive correlation between using WhatsApp for academic support and student retention, ultimately leading to enhanced success rates. The study used the 'Academic Dropout Wheel, a theoretical framework developed by Naaman (2021), which identifies key factors contributing to academic dropout and provides strategies for intervention. The results of a quantitative questionnaire on 181 of the 2022 cohort of FTENS at the campus revealed that students felt more at ease, had a feeling of belonging and ownership of the university, and knew where to ask for help through the effective use of WhatsApp. This paper concludes that if professionally managed, WhatsApp can be an effective tool to facilitate continued adjustment and integration of FTENs into the university.

Abstract The ball bearing market is mature where there is a massive range of products available with new ones being developed all the time due to technological advancements. Additive Manufacturing (AM) provides a promising approach for developing oil-impregnated ball bearings. Oil impregnated bearings are critical for applications requiring smooth and low-friction motion. This study explores the feasibility of utilizing laser powder bed fusion (LPBF) technology to fabricate 304 stainless steel (304SS) samples with open pores, which can then be impregnated with a lubricant. To achieve this, 304SS powder was used, and optimum selective laser melting (SLM) printing parameters were altered to induce intentional pores. Initial screening of samples involved Archimedes density measurements and computed tomography (CT) scanning was conducted on a selected samples to assess their porosity levels. CT scan foam analysis results indicated a correlation between hatch spacing and porosity. Results revealed trends in cell volume and solidified scanning tracks thickness, indicating greater connectedness with larger pores. Synthesis of these findings could help in the development of efficient and reliable open pores that may find use in oil-impregnated self-lubricating ball bearings.

Abstract Haematocrit Levels is defined as the proportion, by volume, of the blood that consists of red blood cells. This study was conducted to model the progress of haematocrit levels over time in kidney patients after their transplant and to determine how the progress depends on the age and gender of the patient and other factors. This is a longitudinal study, and haematocrit level was considered as a response while the covariates were time, gender, age, cardio-vascular problem during the years preceding the transplantation, and rejection symptoms during the first three months after the transplantation took place of the patients. Different statistical methods such as, multivariate regression model, two stage analysis and linear mixed effects model were employed to explore the progress of haematocrit over time. Models revealed that haematocrit levels in kidney transplant patients evolve over time. Gender and Age of the patient have significant effect on the evolution of haematocrit levels. Males tend to have a high increase in haematocrit levels over time than females. With regards to age, haematocrit levels tend to increase with increasing age. Moreover, it was observed that experience of cardio-vascular problems before trandplant and rejection symptoms did not have a significant effect on the progress of haematicrit levels. The growth of Haematocrit levels evolve over time follows a quartic time effect. Change in haematocrit levels varies according to gender and age of the patient. Patients starting with low haematocrit levels tend to have a larger increase over time.

It is essential to ensure differentiation in curriculum delivery to give all learners access to learning while also meeting the diversity of learner needs in the classroom in line with the Republic of South Africa’s inclusive education policy. This mixed methods study sought to examine grade 6 teachers’ knowledge and understanding of differentiated instruction to respond to the diverse needs of learners in OR Tambo Education District in the Eastern Cape. Data was collected from three randomly selected primary schools using interviews, questionnaires, and classroom observation tools. Four grade 6 teachers for mathematics and reading-to-learn were selected from each school (N 12). Key findings revealed that teachers lacked knowledge and understanding of differentiated instruction, and how it should be implemented in the classroom. This implies that all teachers should be trained or in-serviced (during and after training respectively) on how to respond to learners’ diverse needs during teaching and learning. This study contributes to knowledge in the field of inclusive education. The results of this study may influence universities to design teacher education programmes, methods, and/or interventions, which will enhance responses to learner diversity through differentiated instruction. This study could help researchers uncover the critical areas of differentiated instruction, its importance and its application in the classroom, its benefits for teachers and learners, and its impact on student learning.

Abstract Background The escalation of antimicrobial resistance (AMR) in recent years has been of major public health concern globally. Escherichia coli are amongst the bacteria that have been targeted for AMR surveillance due to their ability to cause infection in both animals and humans. Their propensity to produce extended spectrum beta‐lactamases further complicates the choices of treatment regimens. Objectives To investigate the prevalence of antimicrobial‐resistance in E. coli strains isolated from faecal samples of dogs and cats from selected veterinary surgeries and animal shelters from Harare, Zimbabwe. Materials and Methods A cross‐sectional study was carried out to select animals by a systematic random procedure. Faecal samples were collected for culture and isolation of E. coli. Their susceptibility to antimicrobial drugs was assessed using the disc diffusion method. Results A total of 95% (133/140) of the samples from cats (n = 40) and dogs (n = 93) yielded E. coli. Resistance was recorded for ampicillin (45.9%), trimethoprim‐sulphamethoxazole (44.4%), nalidixic acid (29.3%), ceftazidime (15.8%) and azithromycin (12.8%), but not for gentamicin and imipenem. A total of 18% of the isolates were multi‐drug‐resistant where resistance to nalidixic acid, ampicillin and trimethoprim‐sulphamethoxazole predominated. Conclusion We observed relatively high AMR of E. coli strains against ampicillin. The isolation of multi‐drug‐resistant strains of E. coli may signal the dissemination of resistance genes in the ecosystem of these bacteria which may have a public health impact.

Education serves as a catalyst for achieving Sustainable Development Goals (SDGs), and it is essential for education to keep pace with societal dynamics. This paper argues for the reconceptualisation of initial teacher education in South Africa to promote an alternative transformative and sustainable pedagogy. Questions arose regarding the compatibility of the current teacher education program in South Africa with the needs of today's learners. Therefore, it is important to assess whether South African higher education institutions provide adequate training for future teachers. The current teacher education programme has remained unchanged for many years, failing to adapt to the evolving educational landscape and instead adhering to traditional methods that were relevant in the past. Incorporating flexible, transformative pedagogies and technologies can enhance student learning and engage learners of the 21st century. Using a typological methodological design, this conceptual explanatory paper identifies six themes: program content, pedagogy, technological advancements, preparing teachers for inclusion, school-based experiences, and addressing anxiety. This paper contributes to existing knowledge by advocating for the reconceptualisation of teacher education programs, aiming to produce graduates who are equipped to address global challenges. Furthermore, it emphasises the need for an alternative transformative sustainable initiative to overhaul teacher education programs to align with modern societies' complexities and dynamics. Therefore, this paper calls for the reformulation of South Africa's initial teacher training to align with SDG 4 (quality education) and Agenda 2023, which underpin Africa's transformational aspirations for a better future for all.

WhatsApp as the most popular instant messaging technology that has created opportunities for online cooperation and teamwork among students in the university context [1] and allows students to communicate even outside school hours [2]. Transitioning from high school to university can be a challenging experience for many students, particularly those who are entering a new academic atmosphere with greater autonomy and higher workload [3]. This data publication [4] presents a dataset capturing the perceptions of first-year university students on the utilization of WhatsApp as a means of continuous induction into university life. The dataset includes responses from participants on 7 background questions and 14 Likert scale questions, measuring agreeableness on the academic dimension of the academic dropout wheel [5].The questionnaire aimed to investigate the effectiveness of WhatsApp groups established by academic support staff in facilitating the integration of first-year students into both formal academic structures and informal social systems within the campus. The data may offer valuable insights into the potential benefits of WhatsApp as an educational tool and its impact on fostering a positive academic environment, ultimately helping to reduce dropout rates in institutions of higher learning.Researchers, educators, and policymakers can utilize this dataset to gain a deeper understanding of the role that WhatsApp can play in enhancing student engagement and promoting successful integration into university life. By harnessing the information from this dataset, institutions can develop targeted strategies to provide effective support systems for first-year students, thereby promoting academic success and retention.

This study sought to explore the effectiveness of using the Strategy Instructions Approach (SIA) to teach South African children reading comprehension. The majority of language teachers, especially those who teach English as a first or second language, were found to be lacking in the skills needed to impart reading comprehension effectively to their learners. Grade 8 learners were taught reading comprehension using SIA which was basically from the curriculum and assessment policy statement (CAPS) materials. A control group of 60 learners and an experimental group of 60 learners (sampled from three schools in Amathole East District in South Africa) were used to determine the effectiveness of SIA when compared to the traditional teaching approaches used by teachers to teach reading comprehension. The outcomes demonstrated that SIA enhanced performance in reading comprehension. The study recommends that schools especially, those in rural areas should have more reading resources and activities, longer hours on the school timetable for teaching reading comprehension, and higher institutions should train teachers to use SIA.

Abstract Barrier bednets (BBnets), regular bednets with a vertical insecticidal panel to target mosquitoes above the bednet roof, where they are most active, have the potential to improve existing Insecticidal Treated Bednets (ITNs), by reducing the quantity of insecticide required per net, reducing the toxic risks to those using the net, potentially increasing insecticide choice. We evaluated the performance of PermaNet 3.0 (P3) and untreated (Ut) bed nets with and without pyrethroid and piperonyl butoxide roof barriers in killing pyrethroid-resistant and susceptible Anopheles gambiae, simultaneously video-recording mosquito flight tracks. Bioassay results showed that treated roof barriers, particularly the longitudinal P3 barrier (P3L) could be an effective addition to a bed net: P3 + P3L were consistently significantly more effective than the reference P3 bednet while performance of untreated nets could be raised to equal that of the reference P3 following the addition of a P3 barrier. The BBnet’s potential to augment existing bednets and enhance their performance is considered.

Abstract Background Insecticide-treated nets (ITNs) using pyrethroids have been the main vector control tools deployed in malaria endemic countries and are responsible for the dramatic reduction in African malaria cases in the early 2000s. The World Health Organization (WHO) cone test was designed to assess the rapid toxicity effects of pyrethroid exposure on mosquito vectors but has yielded no insights beyond 60-min knockdown and 24-h mortality. As dual-active-ingredient (AI) ITNs become more widespread, bioassays that can provide realistic assessment of single- and dual-treated ITNs (i.e. nets with more than one active ingredient) are urgently needed. Methods We present an augmentation of the cone test that enables accurate quantification of vector behavioural responses (specifically movement, spatial and temporal occupancy) to ITNs using video recording and bespoke software that uses background segmentation methods to detect spatial changes in the movement of mosquitoes within the cone. Four strains of Anopheles gambiae sensu lato (s.l.) were exposed to four ITNs (PermaNet 2.0, PermaNet 3.0, Olyset Net, Interceptor G2) and untreated nets in these modified cone tests. Life history data (post-exposure blood-feeding, blood meal weight, longevity) for individual mosquitoes were recorded. Results All mosquitoes responded to the presence of ITNs, spending from 1.48 to 3.67 times more time in the upper region of the cone, depending on the ITN type. Of all ITNs, PermaNet 2.0 provoked the smallest change in behavioural response. Activity in the cone influenced observed post-exposure longevity, and in resistant strains exposed to Interceptor G2, the higher the activity, the greater the risk of dying, as long as the proportion of activity at the net surface was less than 50%. All ITNs inhibited blood-feeding, and smaller blood meals were taken when mosquitoes fed. Conclusions The additional mosquito behaviour data obtained by using this modification to the WHO cone test provides unique insight into the innate responses of different mosquito strains on untreated nets and the entomological mode of action of ITNs, important evidence when evaluating ITN characteristics. Graphical Abstract

Abstract Monitoring the presence of commensal and pathogenic respiratory microorganisms is of critical global importance. However, community-based surveillance is difficult because nasopharyngeal swabs are uncomfortable and painful for a wide age range of participants. We designed a methodology for minimally invasive self-sampling at home and assessed its use for longitudinal monitoring of the oral, nasal and hand microbiota of adults and children within families. Healthy families with two adults and up to three children, living in and near Liverpool, United Kingdom, self-collected saliva, nasal lining fluid using synthetic absorptive matrices and hand swabs at home every two weeks for six months. Questionnaires were used to collect demographic and epidemiological data and assess feasibility and acceptability. Participants were invited to take part in an exit interview. Thirty-three families completed the study. Sampling using our approach was acceptable to 25/33 (76%) families, as sampling was fast (76%), easy (76%) and painless (60%). Saliva and hand sampling was acceptable to all participants of any age, whereas nasal sampling was accepted mostly by adults and children older than 5 years. Multi-niche self-sampling at home can be used by adults and children for longitudinal surveillance of respiratory microorganisms, providing key data for design of future studies.

Chronic Kidney Disease (CKD) or renal failure is a public global health problem with an estimated prevalence of as 8 to 16% worldwide. This study was conducted in order to Specify Dropout Models the haematocrit levels over time in renal patients after their transplant. This is a longitudinal study that consisted of 1160 patients who received a renal transplant. Level of Haematocrit level as a response, time (log (years)), gender and age of the patients just to mention a few were as a covariate. Different statistical methods, such as dropout models were employed. Direct likelihood and MI based approach of Linear Mixed Model revealed that all the covariates included in the final models were significant. It was observed that the evolution of haematocrit levels follows a quartic time trend and the effect of time on the evolution varies according to gender and age. The effect of time followed a significant 5 th power time trend under MI GEE and quadratic time trend under WGEE. However, both models are valid under MAR. In addition, age, gender and rejection symptoms have a significant effect on the evolution of haematocrit levels in renal patients, however their effect varies with time.

The obligation to increase the standard of instruction and learning falls on academic developers. A crucial part of academic staff development at institutions of higher learning is the induction of academics who are primarily subject matter specialists without the pedagogical abilities to teach effectively in the higher education environment. This qualitative essay investigated how the Professional Excellence Programme (PEP) might be utilised as an induction Short Learning Programme (SLP) for new academics. After finishing the brief SLP, the inductees were given a questionnaire to evaluate the modules (Teaching and Learning, Curriculum Development, and Assessment in Higher Education) and qualitative data was gathered. The study was framed by Wegner’s social learning theory. The research showed that the PEP programme can be an efficient programme to develop academics into university teachers. Since the SLP was accepted by all participants in the study, the study recommended that the Directorate of Teaching and Learning should develop the PEP programme into a credit-bearing postgraduate diploma in higher education at NQF Level 8, which is equivalent to an honours degree.

Abstract Vector control interventions play a fundamental role in the control and elimination of vector-borne diseases. The evaluation of vector control products relies on bioassays, laboratory and semi-field tests using live insects to assess the product’s effectiveness. Bioassay method development requires a rigorous validation process to ensure that relevant methods are used to capture appropriate entomological endpoints which accurately and precisely describe likely efficacy against disease vectors as well as product characteristics within the manufacturing tolerance ranges for insecticide content specified by the World Health Organization. Currently, there are no standardized guidelines for bioassay method validation in vector control. This report presents a framework for bioassay validation that draws on accepted validation processes from the chemical and healthcare fields and which can be applied for evaluating bioassays and semi-field tests in vector control. The validation process has been categorized into four stages: preliminary development; feasibility experiments; internal validation, and external validation. A properly validated method combined with an appropriate experimental design and data analyses that account for both the variability of the method and the product is needed to generate reliable estimates of product efficacy to ensure that at-risk communities have timely access to safe and reliable vector control products.

Abstract Background Bovine brucellosis and tuberculosis are important zoonoses affecting both livestock and wildlife. Objectives The study aimed to investigate seroprevalence of brucellosis and to isolate Brucella spp. and Mycobacterium bovis in cattle from livestock–wildlife interface areas. Methods Three sites were selected from high, medium and low interface. The high interface was adjacent to the park and separated by a broken fence, while the medium and low interface were 15–20 and 50 km from the perimeter fence, respectively. Cattle aged ≥2 years were randomly selected and bled for serology. Culture for brucellae and Mycobacterium species was attempted on lymph nodes collected at the slaughter floor. Sera were screened for Brucella antibodies using the Rose Bengal test and confirmed by the Complement fixation test. Data were analysed using a multivariable logistic regression model. Results Overall, seroprevalence was 11.7% (125/1068; 95% CI: 9.8–13.6%). High interface areas recorded significantly higher (p < 0.05) seroprevalence of 20.9% (85/406; 95% CI: 17.0–24.9%), compared to low 8.9% (31/350; 95% CI: 5.9–11.8%) and medium interface 2.9% (9/312; 95% CI: 1.0–4.8%). Brucella seropositivity was approximately three times higher (OR = 3.3, 95% CI: 2.1–5.3) for Malipati compared to Chiredzi. Similarly, the odds were twice (OR = 2.0, 95% CI: 1.2–3.5) in cows with history of abortion compared to those without. Brucella and Mycobacterium species were not isolated from all samples. Conclusions The study highlighted the significance of high interface as a nexus for amplification of brucellosis in cattle. Thus, a brucellosis control programme that takes into consideration limiting livestock–wildlife interaction should be considered.

Astroviruses (AstVs) have been discovered in over 80 animal species including diverse bat species and avian species. A study on Astrovirus circulation and diversity in different insectivorous and frugivorous chiropteran species roosting in trees, caves and building basements was carried out at 11 different sites across Zimbabwe. Pooled and individual faecal sampling methods were used for this study, with collection dates ranging from June 2016 to July 2021. In two sites, Magweto and Chirundu, sampling was carried out at monthly intervals from August 2020 to July 2021. Astroviruses and bat mitochondrial genes were amplified using pan-AstVs and CytB /12S RNA PCR systems respectively. Phylogenetic analysis of the RdRp Astrovirus sequences revealed a high genetic diversity of astroviruses. All the bat astroviruses tested in this study clustered with the Mamastrovirus genus. Two distinct groups of amplified sequences were identified. One group was comprised of sequences isolated from Hipposideros, Rhinolophus and Eidolon helvum spp. clustered with Human Astrovirus strains: HuAstV types1-6, HuAstV-MLB1-3 and HuAstV-VA-1. The second group comprising the majority of the sequences clustered with different strains of Bat AstVs. Results from the longitudinal study at Magweto and Chirundu showed an overall AstV prevalence of 13.7% and 10.4% respectively. Peaks of AstV infection at Chirundu coincided with the period when juveniles are 4-6 months old. By combining data from our previous work on Coronaviruses, we also anaylzed co-infection of bats with Coronaviruses and Astroviruses at Magweto and Chirundu sites for which the prevalence of co-infection was 2.6% and 3.5% respectively.

Abstract Background The WHO cone test is one of three tests currently used to evaluate the efficacy of insecticide-treated bed nets (ITNs). It generates two test outputs, knockdown and 24-h mortality, both indicative of immediate toxicity but that reveal little about the nature of mosquito and ITN interaction or how results translate to real-world settings. Methods A human arm held 5 mm behind the net surface acted as a host attractant during cone tests and a smartphone was used to capture mosquito behaviour in the cone. Post-exposure blood feeding and survival for nine days were recorded; ingested blood meal size was determined by measuring excreted haematin. Four strains of Anopheles gambiae (insecticide susceptible: Kisumu and N’gousso; insecticide resistant: Banfora and VK7) were tested with and without the host attractant using untreated, Permanet 2.0 and Olyset nets. Video recordings were scan sampled every five seconds to record mosquito positions on either the net, in flight or in contact with the cone. Generalized estimating equations were used to analyse all data except survival within nine days which was analysed using Weighted Cox Regression. Results Net contact was the most frequently recorded behaviour in all Anopheles spp. strains on all nets. Adding the human host as attractant triggered excitatory behaviours: in all strains, the magnitude of net contact was significantly decreased compared to tests without a host. ITN exposure altered the observed behaviour of the two susceptible strains, which exhibited a decreased response to the host during ITN tests. The resistant strains did not alter their behaviour during ITN tests. Significantly less net contact was observed during Olyset Net tests compared to Permanet 2.0. The host presence affected survival after exposure: Banfora and VK7 mosquitoes exposed to Permanet 2.0 with a host lived longer compared to tests performed without a host. However, mosquitoes that blood-fed and survived long enough to digest the blood meal did not exhibit significantly reduced longevity regardless of the presence of the host attractant. Conclusions Simple modifications to the WHO cone test and extension of post-test monitoring beyond the current 24 h enable detailed behavioural characterizations of individual ITNs to be compiled. The effects observed from testing with a host and including blood feeding suggest that more representative estimates of true of ITN efficacy are gained with these modifications than when using the current testing protocol.

High temperatures and frequent heat waves raise concerns about heat stress in cattle in grass-based systems, especially in arid and semiarid areas. This study analysed the relationship between conformation traits and physiological parameters associated with heat stress in Mashona cattle. A total of 200 records from fifty cows were used to study the relationships between seven conformation traits and physiological parameters associated with heat stress. Body conformation traits were categorised into three principal components related to body capacity (body depth, flank circumference, chest girth), frame size (stature and body length), and loose skin fold (navel height and dewlap size). As the size of abdominopelvic and thoracic cavities increased, respiratory rate, heart rate, and rectal temperature decreased significantly, while blood triiodothyronine concentration increased. Cattle with deeper bodies, larger flanks, and larger chest girths had significantly lower heart rate, respiratory rate, and rectal temperature but higher blood triiodothyronine concentration than cattle with shallower bodies, smaller flanks, and smaller chest girths. Respiratory rate increased with increasing frame size. Large-framed cattle had significantly higher respiratory rate and lower blood thyroxine concentration. Small-framed cattle with larger chest girth, larger dewlap, and navel farther from the ground surface are better adapted to higher ambient temperatures.

The Covid-19 pandemic forced universities into emergency remote learning. For universities situated in a country where internet connectivity prices are high, network coverage is low, and most students live in rural areas, this presented a unique set of problems. One of the issues is that traditional orientations were face-to-face for first-time entering students (FTENs) at universities, and they had to be moved online due to lockdown restrictions and the desire to save the academic year. For the university concerned, this presented challenges in accessing students due to their low digital literacy skills and infrastructure limitations. The study employed a quantitative exploratory research approach to assess whether WhatsApp, a low-cost communication tool offering features like affordable data bundles, group creation, and accessibility, could facilitate student engagement and support during the COVID-19 lockdown at a rural university campus. The aim was to investigate if utilizing WhatsApp could foster a sense of belonging among students, potentially mitigating dropout rates and preventing students from falling behind. The hypothesis posited a positive correlation between using WhatsApp for academic support and student retention, ultimately leading to enhanced success rates. The study used the 'Academic Dropout Wheel, a theoretical framework developed by Naaman (2021), which identifies key factors contributing to academic dropout and provides strategies for intervention. The results of a quantitative questionnaire on 181 of the 2022 cohort of FTENS at the campus revealed that students felt more at ease, had a feeling of belonging and ownership of the university, and knew where to ask for help through the effective use of WhatsApp. This paper concludes that if professionally managed, WhatsApp can be an effective tool to facilitate continued adjustment and integration of FTENs into the university. [ABSTRACT FROM AUTHOR]

Longitudinal, community-based sampling is important for understanding prevalence and transmission of respiratory pathogens. Using a minimally invasive sampling method, the FAMILY Micro study monitored the oral, nasal and hand microbiota of families for 6 months. Here, we explore participant experiences and opinions. A mixed methods approach was utilised. A quantitative questionnaire was completed after every sampling timepoint to report levels of discomfort and pain, as well as time taken to collect samples. Participants were also invited to discuss their experiences in a qualitative structured exit interview. We received questionnaires from 36 families. Most adults and children >5y experienced no pain (94% and 70%) and little discomfort (73% and 47% no discomfort) regardless of sample type, whereas children ≤5y experienced variable levels of pain and discomfort (48% no pain but 14% hurts even more, whole lot or worst; 38% no discomfort but 33% moderate, severe, or extreme discomfort). The time taken for saliva and hand sampling decreased over the study. We conducted interviews with 24 families. Families found the sampling method straightforward, and adults and children >5y preferred nasal sampling using a synthetic absorptive matrix over nasopharyngeal swabs. It remained challenging for families to fit sampling into their busy schedules. Adequate fridge/freezer space and regular sample pick-ups were found to be important factors for feasibility. Messaging apps proved extremely effective for engaging with participants. Our findings provide key information to inform the design of future studies, specifically that self-sampling at home using minimally invasive procedures is feasible in a family context.

United Nations (UN) sustainable development goal 6 focuses on clean water and sanitation for all. To date, one-third of the world’s population lack access to clean, safe water. The traditional water purification methods cannot monitor and control water quality parameters from the early stages. In this paper, Internet of Things (IoT) is used to develop a real-time water quality monitoring and control system for a water treatment plant. A microcontroller is developed and interfaced with sensors to detect any deviations in the water quality parameters from the World Health Organization (WHO) standards. Every stage of the water treatment process is tested for quality, and deviations from the WHO standard triggers an autonomous control measure. A simulation model and a working prototype are developed. The research produces the expected results: accurate monitoring and control of water parameters.

The study sought to answer: How can participatory and visual methodology enable women teachers to lead change against intimate partner violence (IPV) at a state university in Zimbabwe? The study was qualitative and informed by the critical paradigm and employed visual participatory research methodology. Six women educators from a state university were selected by means of snowballing. The methods of data generation that were employed were drawings and focus group discussions. Data generated from the drawings depicted the participants’ positioning and how they are positioned in intimate relationships. The findings indicated that the participants understand intimate partner violence as a complex issue and through their voices can initiate change by breaking down barriers as they address the issue. The participants also indicated that gendered social structures affect intimate partner relationships. The women educators are actors who are aware of intimate partner violence and can participate and inform intervention programs. The study concludes that using participatory visual methodology (PVM) provided a safe space for the women to talk about intimate partner violence and propose solutions to a phenomenon that has always been a taboo.

Outsourcing manufacturing to a contract manufacturer is a growing practice that has gained global prominence since the turn of the century. This business practice is common in pharmaceutical and electronics industries, and has now also become common practice in consumer product supply chains. This practice has many challenges; in most cases it does not achieve its intended objectives, with a number of contract manufacturing companies (CMCs) in South Africa going out of business. Research and the literature on contract manufacturing is predominantly focused on the aspects of contract manufacturing and its role and impact in the supply chain. However, there are very few, if any, scholarly articles on the factors that make CMCs in the consumer products supply chain in South Africa succeed and become sustainable businesses and strategic partners to their brand owner customers. This study aimed to fill this gap by identifying, through empirical research of experts in contract manufacturing, the factors that are critical for contract manufacturers to succeed and to be sustainable businesses that can contribute to the success of their clients. Several critical success factors were identified using a Delphi survey of experts who had experience working with CMCs. [ABSTRACT FROM AUTHOR]

This paper presents a methodological approach to conducting a literature review on additive manufacturing (AM), which is a groundbreaking manufacturing technique that involves the successive layering of materials to create components. The use of AM not only reduces manufacturing waste but also saves time and simplifies assembly processes. The current research focused on exploring the advances of AM in fabricating self-lubricating components. By using specific keywords, relevant data sources were extracted from databases. The literature review highlights the extensive exploration of AM in tribological research, demonstrating its successful application in creating self-lubricating components that have yielded positive results. [ABSTRACT FROM AUTHOR]

Background: The escalation of antimicrobial resistance (AMR) in recent years has been of major public health concern globally. Escherichia coli are amongst the bacteria that have been targeted for AMR surveillance due to their ability to cause infection in both animals and humans. Their propensity to produce extended spectrum beta‐lactamases further complicates the choices of treatment regimens. Objectives: To investigate the prevalence of antimicrobial‐resistance in E. coli strains isolated from faecal samples of dogs and cats from selected veterinary surgeries and animal shelters from Harare, Zimbabwe. Materials and Methods: A cross‐sectional study was carried out to select animals by a systematic random procedure. Faecal samples were collected for culture and isolation of E. coli. Their susceptibility to antimicrobial drugs was assessed using the disc diffusion method. Results: A total of 95% (133/140) of the samples from cats (n = 40) and dogs (n = 93) yielded E. coli. Resistance was recorded for ampicillin (45.9%), trimethoprim‐sulphamethoxazole (44.4%), nalidixic acid (29.3%), ceftazidime (15.8%) and azithromycin (12.8%), but not for gentamicin and imipenem. A total of 18% of the isolates were multi‐drug‐resistant where resistance to nalidixic acid, ampicillin and trimethoprim‐sulphamethoxazole predominated. Conclusion: We observed relatively high AMR of E. coli strains against ampicillin. The isolation of multi‐drug‐resistant strains of E. coli may signal the dissemination of resistance genes in the ecosystem of these bacteria which may have a public health impact. [ABSTRACT FROM AUTHOR]

Abstract Background Malaria-endemic countries distribute long-lasting insecticidal nets (LLINs) through combined channels with ambitious, universal coverage (UC) targets. Kenya has used eight channels with variable results. To inform national decision-makers, this two-arm study compares coverage (effects), costs, cost-effectiveness, and equity of two combinations of LLIN distribution channels in Kenya. Methods Two combinations of five delivery channels were compared as ‘intervention’ and ‘control’ arms. The intervention arm comprised four channels: community health volunteer (CHV), antenatal and child health clinics (ANCC), social marketing (SM) and commercial outlets (CO). The control arm consisted of the intervention arm channels except mass campaign (MC) replaced CHV. Primary analysis used random sample household survey data, service-provider costs, and voucher or LLIN distribution data to compare between-arm effects, costs, cost-effectiveness, and equity. Secondary analyses compared costs and equity by channel. Results The multiple distribution channels used in both arms of the study achieved high LLIN ownership and use. The intervention arm had significantly lower reported LLIN use the night before the survey (84·8% [95% CI 83·0–86·4%] versus 89·2% [95% CI 87·8–90·5%], p

Abstract Background Insecticide-treated nets (ITNs) are losing efficacy against pyrethroid-resistant malaria vector populations throughout Africa. Safeguarding bed net efficacy, vital for effective malaria control, requires greater knowledge of mosquito–ITN interactions and how this impacts on the mosquito. Methods A purpose-built benchtop apparatus with a closed 10 cm cubic chamber (the ‘Baited-box’) was used to video record behaviour of individual free-flying female Anopheles gambiae during approach and blood-feeding on a human hand through untreated nets and ITNs at close range. Time and duration of defined behavioural events, and knockdown and mortality at 1- and 24-h post-exposure respectively, were recorded for pyrethroid susceptible and resistant mosquitoes. Results Using three human volunteers differing in relative attractiveness to mosquitoes, 328 mosquitoes were individually tested. There were no significant differences between response rates to ITNs and untreated nets (P > 0.1) or between resistant (Tiassalé) and susceptible (Kisumu) mosquito strains, at untreated nets (P = 0.39) or PermaNet 2.0 (P = 1). The sequence of behavioural events from host-seeking to completion of blood-feeding was consistent in all tests but duration and start time of events involving net contact were reduced or delayed respectively with ITNs. Blood-feeding durations at untreated nets (means from 4.25 to 8.47 min (95% confidence interval (CI) = 3.39–9.89) at 3 human volunteers) were reduced by 37–50% at PermaNet 2.0, in susceptible (mean 2.59–4.72 min, 95% CI = 1.54–5.5, P = 0.2), and neither ITN type showed detectable spatial repellency. After initial contact, blood-feeding commenced later at Olyset (mean 2.76 min, 95% CI = 1.74–3.76, P = 0.0009) and PermaNet (mean 2.4 min, 95% CI = 1.52–3.33, P = 0.0058) than untreated netting (mean 0.68 min, 95% CI = 0.42–0.94). Conclusions The baited box offers a simple method for detailed characterization of mosquito behavioural responses to insecticidal nets, for comparing entomological modes of action between nets and for defining the behavioural responses of particular mosquito strains or populations. The device has potential as a screening assay in the search for novel net treatments and for investigations into behavioural resistance mechanisms.

There has been burgeoning interest in plant-based feed additives following restrictions placed on the use of antibiotic feed additives in many countries. Phytogenic feed additives are recommended to have a range of useful properties to support the growth and development of poultry to a similar level as that obtained by supplementing feed with antibiotics. The aim of this study was to evaluate the antibacterial, anti-lipoxygenase and antioxidant activity, and in vitro safety of fractions and isolated compounds from leaves of Senna singueana. Antibacterial activities of the fractions and isolated compounds were determined against a panel of bacteria using a two-fold serial microdilution assay and qualitative bioautography assays. Anti-lipoxygenase activity was evaluated using the ferrous oxidation-xylenol orange (FOX) method. Antioxidant activity was assessed qualitatively and quantitatively using radical scavenging assays. Dichloromethane and ethyl acetate fractions from solvent-solvent partitioning had the best antibacterial activity with MIC values ranging from 156 to 313 μg/ml. Fractions obtained from column chromatography had significant to weak antibacterial activity with MIC values ranging from 50 to 1,250 μg/ml. Bioautography showed clear bands of bacterial inhibition, indicating the presence of a number of active compounds in several fractions. The ethyl acetate fraction and all the tested column fractions had potent anti-lipoxygenase activity with IC50 values of ≤2.5 μg/ml which were lower than that of quercetin (positive control), indicating anti-inflammatory potential. The ethyl acetate fraction and several column fractions had powerful antioxidant activity with IC50 values of ≤5 μg/ml in the ABTS assay. Cytotoxicity values against Vero kidney cells ranged from LC50 = 40.0–989.3 μg/ml. Bioassay-guided fractionation led to the isolation and identification of a known bioactive compound, luteolin. S. singueana is a promising candidate for the development of poultry phytogenic feed additives.

This article discusses the Quality Management (QM) implementation at South Africa’s Nuclear Energy Cooperation (Necsa) for the reconditioning of Ti6Al4V. The reconditioning serves to spheroidise Ti6Al4V from selective laser melting. The quality management tool was implemented to ensure the consistent output of the spheroidised Ti6Al4V. Steps taken to spheroidise Ti6Al4V were documented, and the tools and resources required to ensure the process flows smoothly were highlighted. Standard operating procedures were developed to provide a guide for operators of the spheroidisation facility on the operating parameters needed to consistently spheroidise Ti6Al4V. The main process parameter investigated was the relationship between power and spheroidisation and vaporisation. There are two types of process parameters for effective reconditioning of Ti6Al4V. There are the parameters that operate with the aim of ensuring consistent quality, and the second set ensures economic reconditioning of Ti6Al4V. QM would also ensure that there are standard operating procedures developed within an organisation. For the research, the operating parameters to economically spheroidise Ti6Al4V were determined-