Search

To evaluate and compare the effect and efficacy of linezolid, vancomycin and ciprofloxacin on enterococcal induced biofilm using scanning electron microscopy. Fifty mandibular premolars were selected for use in this study. Access cavity preparation was done. Working length was determined using periapical radiographs. Coronal third of the canals were flared using Gates Glidden drills. The canals were prepared up to size # 25 K file, 1mm short of apical foramen. Step back preparation was completed up to size # 50 K file. During instrumentation of the canals, canals were irrigated using sodium hypochlorite 5.25% and 17% EDTA. The teeth were then air-dried and steam autoclaved at 121°C for 30 minutes. Two longitudinal grooves were made along the entire length on opposite sides of the outer surface of the teeth to act as a guide for the subsequent splitting of the tooth into two halves. All the samples were divided into 5 groups (n=10). GROUP 1: Negative control. GROUP 2:Positive control. GROUP 3: Linezolid. GROUP 4: Vancomycin. GROUP 5: Ciprofloxacin. Residual aliquots were removed and spread on brain heart infusion broth and send for SEM to determine the percentage of negative culture obtained. Results were analysed using one-way ANOVA test and Duncan's test. A p-value less than 0.05was considered significant. The antimicrobial efficacy of linezolid was found to be higher than that of ciprofloxacin and vancomycin. [ABSTRACT FROM AUTHOR]

Aim: The aim is to test the antimicrobial activity of Andrographis Paniculata, Azadirachta indica (neem), and Curcuma Longa (curcumin) as a root canal irrigant, against Staphylococcus aureus and Candida albicans using agar diffusion test. Sodium hypochlorite (NaOCl) served as a standard control for comparisons. Materials and Methods: The bacterial strains of C. albicans and S. aureus culture were grown overnight (18–20 h) in the brain heart infusion broth at 37°C and inoculated in Mueller–Hinton agar plates. Antibacterial inhibition was assessed using agar well-diffusion method using the methanolic extracts of the three plants to be tested and NaOCl. Bacterial inhibition zone around each well was recorded. The results were tabulated and analyzed statistically for significance. Results: The novel A. paniculata showed significantly higher zone of inhibition against C. albicans (P < 0.0001) compared to the experimental irrigants. Against S. aureus, it exhibited similar results as that shown by NaOCl (P > 0.05). Conclusion: Zones of inhibition exhibited by novel herbal agent A. paniculata were higher against C. albicans and similar against S. aureus, when compared to NaOCl. [ABSTRACT FROM AUTHOR]

Background: The diagnosis of nondisplaced longitudinal fractures [vertical root fractures (VRFs)] is challenging in clinical practice. Radiographic techniques showed a difficulty in detection of VRFs. Cone-beam computed tomography (CBCT) is a new diagnostic imaging modality that provides high-quality three-dimensional (3D) images for dental diagnosis., Aims: The aim of this in vitro study is to compare accuracy of three different imaging modalities: conventional periapical radiographs, digital radiographs, and CBCT in detecting VRFs in teeth that are endodontically as well as nonendodontically treated., Materials and Methods: An in vitro model consisting of 60 recently extracted human mandibular lower premolars were used. Root canal treatment was carried out for 30 teeth. Root fractures were created in 30 teeth (15 root canal treated and 15 non-treated) by mechanical force. Other 30 teeth remain intact. The teeth were mounted and images were taken with a periapical, digital, and CBCT X-ray unit. Three endodontists separately evaluated the images., Results: Interobserver κ values showed a very good interobserver agreement (0.98 for CBCT, 0.88 for digital, and 0.93 for conventional periapical X-rays). There was an overall statistically significant difference ( p = 0.00) in detecting of root fracture among the three imaging modalities and the highest accuracy with CBCT images., Conclusions: In in vitro model, CBCT scan appears to give the highest accuracy in detecting VRFs when compared with the periapical systems in both endodontically and nonendodontically treated teeth., Clinical Significance: The CBCT scan shows higher sensitivity in detection of VRFs in comparison with periapical images.